CN114924008A - Decision method for extracting technological parameters of classical famous prescription Ling Gui shu gan Tang - Google Patents

Decision method for extracting technological parameters of classical famous prescription Ling Gui shu gan Tang Download PDF

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CN114924008A
CN114924008A CN202210610770.2A CN202210610770A CN114924008A CN 114924008 A CN114924008 A CN 114924008A CN 202210610770 A CN202210610770 A CN 202210610770A CN 114924008 A CN114924008 A CN 114924008A
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poria
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梁新丽
康兴东
廖正根
李小锋
温佳豪
胡吉忠
刘厚权
陈梁
夏淑英
周朝忠
罗祎明
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JIANGXI PUZHENG PHARMACEUTICAL CO Ltd
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Abstract

The invention belongs to the technical field of traditional Chinese medicine pharmacy, and particularly relates to a decision method for extracting technological parameters of a classical famous prescription of a poria, cassia, rhizoma atractylodis and rhizoma glycyrrhizae decoction. Determining key quality attributes and key factors of the extraction process of the Lianggui zhuyu decoction by methods such as literature investigation and research, designing and extracting the process by utilizing an orthogonal test, calculating the similarity of the key quality attributes of the ancient decoction and orthogonal test results of the Lianggui zhuyu decoction by adopting a reference correlation degree, determining subjective and objective weight coefficients of each index by combining a layer analysis method and an entropy weight method, further performing comprehensive scoring on the reference correlation degrees under different process parameters, and screening out modern extraction process parameters which are closer to the key quality attributes of the traditional decoction; taking 85-115% of the comprehensive value of the decocted sample of the classic famous formula tuckahoe, cinnamon, atractylodes and licorice decoction as a standard, and deciding technological parameters. The positive progress effects of the invention are as follows: the poria cocos, cassia twig and rhizoma atractylodis macrocephalae decoction prepared based on modern extraction process parameters decided by the method has high consistency of key quality attributes with the poria cocos, cassia twig and rhizoma atractylodis macrocephalae decoction prepared by an ancient method, can effectively and comprehensively ensure scientific inheritance of the poria cocos, cassia twig and rhizoma atractylodis macrocephalae decoction, and ensures the stability and controllability of product quality.

Description

Decision method for extracting technological parameters of classical famous prescription Ling Gui shu gan Tang
Technical Field
The invention belongs to the technical field of traditional Chinese medicine pharmacy, and particularly relates to a decision method for extracting process parameters of a classic famous formula Lingguizhugan decoction.
Background
The formula composition, preparation method and taking method of the tuckahoe four two cassia twig three two rhizoma atractylodis macrocephalae three two liquorice two and the right four flavors are that six liters of water are used to boil the tuckahoe four two cassia twig three two rhizoma atractylodis macrocephalae three two liquorice two and the right four flavors are used, three liters are taken separately, and urination is facilitated. ", is a typical prescription for treating spleen deficiency phlegm-fluid retention disease. In the directory of ancient classical famous parties (first lot) published by the State administration of traditional Chinese medicine in 2018, party 19 is shown. The basic source, the processing and the weighing conversion are listed in a key information table of an ancient classic name prescription, the preparation method of the decoction only comprises the amount and the boiling degree of water, the soaking time and the fire power for decocting are not clear, and the consistency of the quality of the tuckahoe, cinnamon, atractylodes and licorice decoction products is difficult to guarantee.
The development of ancient classical famous prescriptions still has many critical problems to be solved, such as the transformation from the traditional decoction mode to the modern extraction mode. At present, methods such as orthogonal experimental design, Box-Behnken response surface method, uniform design and the like are adopted to carry out experimental design on the extraction process of traditional Chinese medicines or traditional Chinese medicine compounds, key component content and cream yield are used as indexes, and methods such as a combination analytic hierarchy process, an entropy weight method, index correlation and the like are used for carrying out weight determination on each index and calculating a comprehensive score to optimize the extraction process, but the best extraction process is determined by using the better component content, which is greatly different from the core idea of ensuring that the clinically used medicinal substances are basically consistent with the medicinal substances of the traditional decoction in the declaration data requirement (finding of an opinion draft) of the material standard of the traditional Chinese medicine compound preparation of the ancient classical prescription published by the State drug administration. Therefore, the classical name-side extraction process cannot only aim at the maximum extraction rate of index components to decide parameters, and simultaneously, the consistency of the index components and the key quality attributes of the traditional decoction needs to be considered.
The traditional famous prescription is developed into granules, which is one of effective methods for solving the problem that the traditional decoction is not suitable for long-term storage. The extraction process is the source of the difference between the quality attributes of the granules and the reference substances. Factors influencing the extraction process mainly comprise medicinal decoction pieces, extraction equipment, production environment and extraction conditions, wherein the extraction conditions are key factors for process investigation. The quality is derived from the design concept that the relation among raw materials, process and finished product quality is emphasized, and the method has better guiding significance for controlling the extraction process.
Disclosure of Invention
The method aims to solve the problem that the extraction process parameter decision method cannot guarantee the consistency of key quality attributes of the classical famous formula Lingguizhugan decoction. The invention provides a decision method for extracting process parameters of a classical famous prescription Ling Gui shu gan Tang.
A decision method for extracting technological parameters of a classic famous prescription Ling Gui shu gan Tang is characterized by comprising the following steps:
determining key quality attributes of the extraction process of the Lingguizhugao decoction through literature research and fingerprint spectrum research, and determining key factors of the extraction process of the Lingguizhugao decoction through literature research and fishbone map analysis;
decocting by adopting an ancient method of classical famous formula poria cocos, cassia twig, bighead atractylodes rhizome and licorice decoction, detecting decoction liquid, and obtaining key quality attribute information of the ancient method decoction of the poria cocos, cassia twig, licorice decoction;
taking key factors of the extraction process of the poria and cassia twig decoction and the licorice root decoction as investigation objects, carrying out orthogonal test design, and obtaining key quality attribute information of the extraction process of the orthogonal test;
calculating the similarity of key quality attribute information of the ancient decoction and orthogonal test extraction processes of the poria, cassia, bighead atractylodes rhizome and licorice decoction by adopting the reference correlation degree;
combining an analytic hierarchy process and an entropy weight process to determine subjective weight coefficients and objective weight coefficients of all indexes, calculating combination weights through the subjective and objective weights to further comprehensively grade the reference association degrees under different process parameters, and screening out parameters of the modern extraction process which are closer to the key quality attributes of the traditional decoction method of the poria and cassia operculata and gantang;
taking 85-115% of the comprehensive value of the decocted sample of the classic famous formula tuckahoe, cinnamon, atractylodes and licorice decoction as a standard, and deciding technological parameters.
In the technical scheme, the determined key quality attributes are the contents of liquiritin, cinnamic acid and glycyrrhizic acid, and the cream yield and the fingerprint similarity.
In the technical scheme, the key factors of the determined extraction process are soaking time, extraction time and the amount of water added.
In the above technical solution, the reference relevance SR i，j The calculation formula of (2) is as follows: SR i，j ＝(1-|X i，j -S j |/S j )×100％。
Wherein: x i,j Represents the measurement at the jth index of the ith sample of the quadrature test,S j the measurement value at the j-th index of the reference sample is shown.
In the above technical solution, the subjective weight coefficient W i The calculation formula of (2) is as follows:
Figure BDA0003670778150000021
wherein: omega i ′＝(a i1 a i2 …a in ) 1/n ，a ij And establishing the ratio of the importance of the factor i to the importance of the factor j in the judgment matrix for the analytic hierarchy process.
In the above technical solution, the objective weight coefficient W i The calculation formula of (c) is:
Figure BDA0003670778150000022
wherein:
Figure BDA0003670778150000023
k is 1/lnm; while
Figure BDA0003670778150000024
In the above technical solution, the calculation formula of the combination weight coefficient W is:
Figure BDA0003670778150000025
in the above technical solution, the calculation formula of the composite score is:
composite score SR i, glycyrrhizin ×32.81％+SR i, cinnamic acid ×13.95％+SR i glycyrrhizic acid ×46.25％+SR i, extract yield ×6.98％+SR i, fingerprint similarity ×0.1％
The positive progress effects of the invention are as follows: the invention establishes a decision method for extracting process parameters of the tuckahoe, cassia twig, atractylodes rhizome and licorice decoction, the tuckahoe, cassia twig, atractylodes rhizome and licorice decoction prepared based on modern extraction process parameters decided by the method has high consistency of key quality attributes, scientific inheritance of the tuckahoe, cassia twig, atractylodes rhizome and licorice decoction prepared by an ancient method can be effectively and comprehensively ensured, and the stability and controllability of the product quality are ensured.
Drawings
FIG. 1 is an HPLC chart of each sample at 237nm in example 1.
FIG. 2 is a HPLC chart of each sample at 280nm in example 1.
FIG. 3 is a HPLC chart of each sample at 280nm in example 1.
FIG. 4 is a diagram of fishbone screening according to the extraction process parameters of the Ling Gui shu gan Tang of example 1.
Detailed Description
The invention is further illustrated by the following examples, which are not intended to limit the scope of the invention.
Example 1
1. Ancient decoction of Linggui-Gui-Zhu-gan decoction
Soaking 55.2g of poria cocos in 1200mL of water for 1h in a ceramic pot, adding 41.4g of cassia twig, 41.4g of bighead atractylodes rhizome and 27.6g of liquorice, soaking for 30min, boiling with 1800W of strong fire, decocting with 800W of slow fire to about 600mL, and filtering with 200-mesh nylon gauze to obtain the poria cocos-cassia twig-rhizoma atractylodis-sweet soup water decoction.
2. Determination of cream yield
Precisely sucking 50mL of the extract of the decoction of poria, cassia, atractylodes, rhizoma atractylodis and licorice, placing the extract in an evaporation dish with dried constant weight, evaporating the extract in a water bath to dryness, drying the extract at 105 ℃ to constant weight, and calculating the cream yield.
The paste yield is 50mL of the dry paste mass of the extracting solution multiplied by the total volume of the extracting solution/(50 multiplied by the total dosage) multiplied by 100 percent
3. Content determination of index component
3.1 chromatographic conditions
A Shim-pack VP-ODS C18 chromatographic column, 4.6mm × 250mm × 5 μm, acetonitrile as a mobile phase A, 0.1% phosphoric acid water as a mobile phase B, a gradient elution program shown in Table 1, a flow rate of 1mL/min, a detection wavelength, a fingerprint spectrum of 280nm, liquiritin and glycyrrhizic acid contents of 237nm, cinnamic acid contents of 290nm, a column temperature of 30 ℃, and a sample injection of 10 μ L.
TABLE 1 gradient elution procedure
Time A％ Time A％
0～10min 5～10 38～40min 22～30
10～20min 10～20 40～55min 30～43
20～30min 20 55～65min 43
30～38min 20～22 65～70min 43～100
3.2 preparation of control solutions
Precisely weighing liquiritin, cinnamic acid and ammonium glycyrrhizinate reference substances in a measuring flask, adding methanol to constant volume, respectively preparing 0.806mg/mL liquiritin, 1.22mg/mL cinnamic acid and 0.806mg/mL ammonium glycyrrhizinate reference substance solutions, respectively transferring the reference substance solutions in proper amounts, and uniformly mixing to obtain a mixed reference substance solution containing 0.1612mg/mL liquiritin, 0.1220mg/mL cinnamic acid and 0.5160mg/mL ammonium glycyrrhizinate.
3.3 preparation of test solutions
Centrifuging at 20 deg.C and 15000r/min for 10min to obtain supernatant of 0.45 μ m Filtering with microporous membrane to obtain test solution.
3.4 specificity test
Negative water decoctions of Poria, ramulus Cinnamomi, Atractylodis rhizoma and Glycyrrhrizae radix under item "1" are respectively prepared, and negative test sample solutions under item "3.3" are prepared. The reference solution, the whole formula test solution and the negative test solution are tested according to the chromatographic condition under the item of 3.1, and the result is shown in figure 1.
3.5 Linear relationship investigation
Taking a reference substance solution under the item '3.2', adding methanol to dilute the reference substance solution by 2, 4, 8, 16 and 32 times respectively, measuring according to chromatographic conditions under the item '3.1', drawing a standard curve by taking the mass concentration of each index component in the reference substance solution as a horizontal coordinate (X) and the peak area as a vertical coordinate (Y), performing linear regression analysis, and calculating a linear regression equation of each index component to obtain a linear regression equation of liquiritin, wherein the linear regression equation of the liquiritin is that Y is 19.759X-3.5801, R2 is 0.9998, and the linear range is 5.04-161.20 mg/L; the linear regression equation of the cinnamic acid is that Y is 68.48x-13.592, R 2 0.9998, and the linear range is 3.81-122.00 mg/mL; the linear regression equation of glycyrrhizic acid is 5.3812x-4.0719, R 2 The linear range is 16.13-516.00 mg/mL, and the linear range is 0.9998.
3.6 precision test
Taking the same mixed reference substance solution, continuously injecting samples for 6 times according to the chromatographic condition under the item of 3.1, and respectively calculating the RSD values of the areas of liquiritin, cinnamic acid and glycyrrhizic acid to be 1.13%, 1.14% and 1.11%, which indicates that the precision of the instrument is good.
3.7 stability test
Taking 1 part of the test solution, respectively measuring 0, 2, 4, 8, 12 and 24 hours after preparation according to the chromatographic condition under the item of 3.1, respectively calculating the RSD values of the areas of the liquiritin, the cinnamic acid and the glycyrrhizic acid peaks to be 0.47%, 0.38% and 0.63%, respectively, and showing that the test solution has good stability within 24 hours.
3.8 repeatability test
6 parts of test solution are prepared in parallel by taking the same batch of prescription, the determination is carried out according to the chromatographic condition under the item 3.1, and the RSD values of the areas of the liquiritin, the cinnamic acid and the ammonium glycyrrhizinate are respectively calculated to be 1.43 percent, 1.55 percent and 0.42 percent, which shows that the method has good repeatability.
3.9 sample application recovery test
Taking 6 parts of water decoction with known index component content, respectively and precisely adding reference substance solution with the same content, operating according to the method of 3.3 to obtain sample-adding test solution, and respectively calculating average sample-adding recovery rates of the liquiritin, the cinnamic acid and the glycyrrhizic acid to be 100.23%, 100.53% and 99.30% according to the chromatographic condition of 3.1.
4. Determination of key quality attributes and key process parameters
4.1 determination of Key quality Attribute
In combination with research on clinical pharmacological activity literature of the Linggui shugan decoction, content determination in 2020 edition of Chinese pharmacopoeia and research on fingerprint, the content of liquiritin, cinnamic acid and glycyrrhizic acid, and the cream yield and fingerprint similarity are used as key quality attributes of the extraction process of the Linggui shugan decoction.
4.2 screening of Key Process parameters
The key technological parameters of the Linggui shugan decoction are primarily screened by adopting a fishbone picture, and the result is shown in the attached figure 3. And selecting soaking time, extraction time and the amount of water added as key factors of the extraction process by combining literature analysis.
5. Selection of extraction Process factor level
5.1 determination of the amount of Water to be added
Through investigation on the water absorption capacity of the whole formula, the water absorption capacity of the whole formula of the tuckahoe, cinnamon, atractylodes and licorice decoction is 300mL, and the decoction end point is combined with the ancient method: six liters of water is used, three liters are boiled, and the modern measurement is 600mL after the conversion of the three liters; the adding amount of the whole formula of medicinal materials is 165.6g, the water-decocting liquid volume at the end of decoction and the water absorption amount of the medicinal materials are obtained, and the amount of the water-adding times is 5.4 times of the adding amount of the whole formula of medicinal materials, so that the lowest level of the selected water-adding times is 5 times of the adding amount of the whole formula of medicinal materials. Finally, the water adding amount is set to three levels of 5 times, 7 times and 9 times.
5.2 determination of level of soaking time
According to the management specification of a traditional Chinese medicine decoction room of a medical institution and the combination of the early-stage research foundation, the soaking time is set to three levels of 30min, 45min and 60 min.
5.3 determination of extraction time level
Earlier researches show that after the decoction decocted in an ancient ceramic pot is heated and boiled with strong fire, the decoction is continuously decocted with slow fire for 30min to reach the decocting end point to obtain 600mL of decoction, so the lowest level of the extraction time is selected as extraction for 20 min; the extraction time is set at three levels of 20min, 40min and 60 min.
6 Quadrature experimental design
Weighing 9 parts of the same batch of medicinal decoction pieces according to the same prescription and quantity as the ancient method, taking 3 factors of soaking time (A), extraction time (B) and water addition quantity (C) as objects to be investigated, taking the content of liquiritin, cinnamic acid and glycyrrhizic acid in an extracting solution, the cream yield and the similarity of a fingerprint spectrum as evaluation indexes, and carrying out tests according to an orthogonal table L9(34), wherein the results are shown in a table 1.
TABLE 1 results of orthogonal experiments and ancient extraction
Figure BDA0003670778150000051
7. Determination of samples
Taking the extract obtained by each orthogonal test and the ancient method for decoction, preparing a test solution according to the method of item 3.3, measuring according to the chromatographic condition under the item 3.1, recording a chromatogram, and calculating the content of each index component, wherein the result is shown in Table 1.
8 feature picture similarity analysis
And (3) introducing the chromatogram under the wavelength of 280nm obtained in the item 7 into a traditional Chinese medicine chromatogram fingerprint similarity evaluation system for analysis, taking the chromatogram obtained by decocting the extracting solution by an ancient method as a comparison chromatogram, performing full-peak matching by adopting median emission with the time window degree of 0.1, and calculating the similarity, wherein the result is shown in table 1.
9 calculation of similarity from reference correlation
9.1 identification of evaluation object
The reference correlation is a key parameter for evaluating the similarity between a certain sample and a reference sample. Comparing the 9 results of the orthogonal test with a reference sample, taking the content of liquiritin, cinnamic acid, liquiritin, the similarity of fingerprint and 5 indexes of cream yield as evaluation indexes, and taking X as an index i,j 、S j As an evaluation object, X i,j Represents the measurement at the j index of the ith sample of the orthogonal test, S j The measurement value at the j-th index of the reference sample is shown.
9.2 calculating the relative deviation value RD i，j
Calculating X by equation (1) i,j Relative to S j RD of i,j The value is obtained. RD i,j The relative deviation at the jth index for the ith sample is shown. The calculation results are shown in Table 2, RD i,j The smaller, represents X i,j Relative to S j The smaller the deviation of (a), the higher the similarity of the sample to the reference sample.
Equation (1) RD i，j ＝|X i，j -S j |/S j
TABLE 2 RD of Quadrature experiments i，j
Figure BDA0003670778150000052
Calculating the reference relevance SR i，j
SR i,j And the reference relevance of the j index of the ith sample is shown. The reference correlation degree is defined as the value calculated according to the formula (2) by taking the mass of a reference sample prepared according to the ancient book record content as a standard value S, the mass of a sample prepared according to modern process parameters as a measured value X and different indexes as evaluation indexes, and is called as the reference correlation degree, and the closer the value is to 100%, the higher the similarity between the sample prepared according to the modern process parameters under the indexes and the reference sample is. The calculation results are shown in Table 3.
Formula (2) SR i，j ＝(1-RD i，j )×100％＝(1-|X i，j -S j |/S j )×100％
TABLE 3 SR of Quadrature experiments i，j
Figure BDA0003670778150000061
10. Calculation of combined weights of analytic hierarchy process and entropy weight method
10.1 analytic hierarchy Process subjective weighting
According to the importance degree of each index, the priority of each index is determined as liquiritin, cinnamic acid, glycyrrhizic acid and extract yield, fingerprint similarity, a hierarchical analysis method theoretical judgment matrix 1-9 scaling method (table 4) is adopted to compare every two of 5 indexes, and the judgment optimization matrix is shown in table 5.
TABLE 4 analytic hierarchy Process construction of judgment matrix Standard
Figure BDA0003670778150000062
TABLE 5 judge preference matrix for index pairwise comparison
Figure BDA0003670778150000063
Calculating and judging the geometric mean of the preferred matrix according to the square root method, and calculating according to a formula (3) to obtain an initial weight coefficient omega i ' then, a normalized weight coefficient W is calculated according to the formula (4) i And obtaining the normalized weight coefficients of liquiritin, cinnamic acid, glycyrrhizic acid, cream yield and fingerprint similarity which are 0.294, 0.059 and 0.059 in sequence. And (5) carrying out consistency test according to the formulas (5), (6) and (7) to obtain a consistency index CI of 0 and a consistency ratio CR of 0 which are both less than 0.1, thereby indicating that the matrix has consistency.
Formula (3) ω i ′＝(a i1 a i2 …a in ) 1/n
Formula (4)
Figure BDA0003670778150000071
Formula (5)
Figure BDA0003670778150000072
Formula (6) CI ═ λ max -n)/(n-1)
Formula (7) CR ═ CI/RI
Note: in the formula a in Representing the comparison result of the ith index factor to the nth index factor; lambda max The RI is the maximum feature root and the degree of freedom index.
10.2 entropy Objective weighting of information
Establishing an original data matrix R [ R ═ X (X) by taking 5 indexes of liquiritin, cinnamic acid and glycyrrhizic acid content, extract yield and fingerprint similarity as evaluation indexes i，j ) m×n ]. Converting the original data matrix R into a probability matrix P, P according to the formula (8) i,j Representing the probability of the ith sample under the jth index.
Formula (8)
Figure BDA0003670778150000073
10.3 calculating information entropy and weight coefficient of each index
Calculating the information entropy (H) of each index according to the formula (9) j ) 0.9957, 0.9982, 0.9939, 0.9954, 0.9999 and H in sequence j The smaller the data dispersion degree under the j index is, the larger the information amount provided by the j index is, and the weight coefficient (W) of each index is calculated according to the formula (10) j ') 0.2564, 0.1090, 0.3614, 0.2727, 0.0005 in order.
Formula (9)
Figure BDA0003670778150000074
Formula (10)
Figure BDA0003670778150000075
10.4 calculating the combining weight coefficients
The combined weight coefficient of each index was calculated according to the formula (11), and the results are shown in table 6.
Formula (11)
Figure BDA0003670778150000076
TABLE 6 Objective weight and Combined weight
Figure BDA0003670778150000077
11. Composite score
The combination weight coefficient is obtained by an analytic hierarchy process-entropy weight method, and the comprehensive score is calculated according to the formula (12), and the result is shown in table 1. According to the result, the test 9 has the highest comprehensive score, and the modern extraction process parameter which is most similar to the ancient method is A through combining the visual analysis result of the comprehensive score 2 B 1 C 1 Soaking in 5 times of water for 45min, and extracting for 20 min.
Formula (12) composite score SR i, glycyrrhizin ×32.81％+SR i, cinnamic acid ×13.95％+SR i glycyrrhizic acid ×46.25％+SR i, extract yield ×6.98％+SR i, fingerprint similarity ×0.1％
12. Proof test
And 3 verification tests are carried out according to the finally determined extraction process, comprehensive scores are calculated, the result is shown in table 7, the index values of the 3 verification tests are within +/-15% of the ancient cooking sample of the poria and cassia twig decoction, and the RSD value of the comprehensive scores is within 3%, so that the stability and feasibility of the selected extraction process are shown.
Table 7 verifies the test results
Figure BDA0003670778150000081

Claims (8)

1. A decision method for extracting process parameters of a classic famous formula Lingguishugan decoction is characterized by comprising the following steps:
determining key quality attributes of the extraction process of the poria, cassia twig and rhizoma atractylodis macrocephalae and licorice root decoction through literature research and fingerprint spectrum research, and determining key factors of the extraction process of the poria, cassia twig and rhizoma atractylodis macrocephalae and licorice root decoction through literature research and fishbone map analysis;
decocting by adopting an ancient method of classical famous formula poria cocos, cassia twig, bighead atractylodes rhizome and licorice decoction, detecting decoction liquid, and obtaining key quality attribute information of the ancient method decoction of the poria cocos, cassia twig, licorice decoction;
taking key factors of the extraction process of the poria and cassia twig-atractylodes rhizome-licorice root decoction as an investigation object, carrying out orthogonal test design, and obtaining key quality attribute information of the extraction process of the orthogonal test;
calculating the similarity of key quality attribute information of the ancient decoction and orthogonal test extraction processes of the poria, cassia, bighead atractylodes rhizome and licorice decoction by adopting the reference correlation degree;
combining an analytic hierarchy process and an entropy weight process to determine subjective weight coefficients and objective weight coefficients of all indexes, calculating combination weights through the subjective and objective weights to further comprehensively grade the reference association degrees under different process parameters, and screening out parameters of the modern extraction process which are closer to the key quality attributes of the traditional decoction method of the poria and cassia operculata and gantang;
taking 85-115% of the comprehensive value of the decocted sample of the classic famous formula tuckahoe, cinnamon, atractylodes and licorice decoction as a standard, and deciding technological parameters.
2. A decision method as claimed in claim 1, wherein the determined key quality attributes are the content of glycyrrhizin, cinnamic acid, glycyrrhizic acid, and cream yield and fingerprint similarity.
3. The decision method of claim 1, wherein the determined key factors of the extraction process are soaking time, extraction time, and the amount of water added.
4. The decision method of claim 1, wherein the reference correlation degree SR i，j The calculation formula of (c) is:
SR i，j ＝(1-|X i，j -S j |/S j )×100％
wherein: x i,j Represents the measurement at the j index of the i sample of the orthogonal test, S j The measurement at the jth index of the reference sample is indicated.
5. The decision method of claim 1 wherein the subjective weighting factor W i The calculation formula of (c) is:
Figure FDA0003670778140000011
wherein: omega i ′＝(a i1 a i2 …a in ) 1/n ，a ij And establishing the ratio of the importance of the factor i to the importance of the factor j in the judgment matrix for the analytic hierarchy process.
6. The decision method according to claim 1, wherein the objective weighting factor W j The formula for calculation of' is:
Figure FDA0003670778140000012
wherein:
Figure FDA0003670778140000013
k is 1/lnm; and then
Figure FDA0003670778140000014
7. The decision method of claim 1 wherein the weighting coefficients W are combined i The calculation formula of (c) is:
Figure FDA0003670778140000015
8. the decision-making method of claim 1, wherein the composite score is calculated by the formula:
composite score SR i, glycyrrhizin ×32.81％+SR i, cinnamic acid ×13.95％+SR i glycyrrhizic acid ×46.25％+SR i, extract yield ×6.98％+SR i, fingerprint similarity ×0.1％。
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