CN113219076A - Preparation method of notopterygium root decoction standard decoction for eliminating dampness and fingerprint spectrum thereof - Google Patents
Preparation method of notopterygium root decoction standard decoction for eliminating dampness and fingerprint spectrum thereof Download PDFInfo
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- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
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- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/86—Signal analysis
- G01N30/8675—Evaluation, i.e. decoding of the signal into analytical information
- G01N30/8679—Target compound analysis, i.e. whereby a limited number of peaks is analysed
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/86—Signal analysis
- G01N30/8675—Evaluation, i.e. decoding of the signal into analytical information
- G01N30/8686—Fingerprinting, e.g. without prior knowledge of the sample components
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Abstract
The invention discloses a preparation method of a notopterygium root decoction standard decoction for eliminating dampness and a fingerprint spectrum thereof, belonging to the technical field of traditional Chinese medicine compound. The preparation of the standard decoction comprises the following steps: pulverizing the above materials respectively, mixing at a certain proportion, adding distilled water, soaking for half an hour, boiling with strong fire, decocting with slow fire, filtering when it is hot, and centrifuging. The standard decoction preparation method provided by the invention can obtain higher cream yield and extraction rate of active ingredients. The establishment method of the fingerprint comprises the following steps: preparing test solution of different batches, preparing reference solution, and measuring and constructing fingerprint. The fingerprint spectrum establishing method provided by the invention has high stability, repeatability and precision, is simple and feasible, has 20 common peaks including the linarin glycoside, the ferulic acid, the ammonium glycyrrhizinate, the vitexin, the notopterygium alcohol, the osthole and the isoimperatorin, and perfects the quality control method of the notopterygium root dampness removing decoction.
Description
Technical Field
The invention belongs to the field of traditional Chinese medicine compound, and particularly relates to a preparation method of a notopterygium root decoction standard decoction and a fingerprint spectrum thereof.
Background
The classical famous prescription is an outstanding representative of Chinese medicine prescriptions, is the essence of traditional Chinese medicine and is the summary of traditional Chinese medicine experience in all generations. Most of traditional medicinal methods are decoction methods, but the traditional decoction methods mainly consider factors such as a decoction apparatus, a pretreatment method, decoction time and the like, and the parameters are fuzzy, so that corresponding scientific basis is lacked as guidance. In addition, the components contained in the Chinese herbal compound are complex, especially the components change after compatible decoction, which brings a lot of difficulties for the quality control of the compound, and the characteristics of multi-component and multi-target administration of the compound cannot be comprehensively reflected only by using single or a plurality of components as indexes to carry out the quality control. The traditional Chinese medicine fingerprint is a comprehensive and quantifiable identification means, can comprehensively reflect the quality of medicinal materials, can simultaneously analyze various components, is suitable for the characteristics of compound fuzziness and integrity, and has wide application in the quality control of the compound.
Notopterygium root decoction for eliminating dampness is one of 100 ancient classic famous prescriptions published by the national traditional Chinese medicine administration in 2018, and is from 'Nei Wao Wai Bian Huo Bian Lun' of the four kingdoms of Jinyuan Li Dongyuan. The original text states: notopterygii rhizoma, radix Angelicae Pubescentis each with one money, rhizoma Ligustici, radix Saposhnikoviae, Glycyrrhrizae radix (preparata), rhizoma Ligustici Chuanxiong each with five parts, fructus Vitics Simplicifoliae three parts, and the mouth of the patient is filled with one part, and two parts of water are decocted to one part, and the decoction is removed, and is taken at warm temperature and hollow for oral administration. The notopterygium root decoction for treating rheumatism is prepared from seven medicinal materials of notopterygium root, radix angelicae pubescentis, ligusticum, divaricate saposhnikovia root, liquorice (roasted), ligusticum wallichii and fructus viticis, is mainly used for treating diseases caused by wind-damp evil and qi obstruction such as rheumatoid arthritis, migraine, scapulohumeral periarthritis and cold in clinic, and has obvious curative effect. In order to realize the standardization of the decoction process of the notopterygium root decoction for eliminating dampness and comprehensively control the quality of the decoction, a preparation method of a standard decoction of the notopterygium root decoction for eliminating dampness and a fingerprint spectrum thereof are provided.
Disclosure of Invention
The invention provides a preparation method of a notopterygium root decoction standard decoction for eliminating dampness and a fingerprint spectrum thereof, and the preparation method comprises the following steps:
1. preparation of standard decoction: respectively pulverizing Notopterygii rhizoma, radix Angelicae Pubescentis, radix Glycyrrhizae Preparata, rhizoma Ligustici Chuanxiong, rhizoma Ligustici, radix Saposhnikoviae, and fructus Vitics Simplicifoliae into certain particle size, and mixing at a certain ratio. Adding a certain volume of distilled water, soaking for half an hour, boiling with strong fire, and decocting with slow fire for a certain time. After the decoction is finished, filtering while the decoction is hot, and centrifuging to obtain a standard decoction. Taking the comprehensive weighted score of the paste yield, the rubusoside extraction yield and the ammonium glycyrrhizinate extraction yield as evaluation indexes, adopting an orthogonal test to investigate the influence of three factors of water addition, decoction time and decoction times on the decoction process, obtaining an optimal decoction process, and carrying out process verification;
2. preparing a fingerprint sample solution: respectively decocting Notopterygii rhizoma decoction to obtain standard decoctions, lyophilizing to obtain powder, adding methanol water solution with certain concentration, dissolving to obtain test solution with certain concentration, and coating with membrane;
3. preparation of control solutions: accurately weighing appropriate amount of linarin, ferulic acid, ammonium glycyrrhizinate, vitexin, notopterygium alcohol, osthole, and isoimperatorin reference substances, adding methanol water solution with certain concentration for dissolving, diluting to 10mL, and filtering with microporous membrane to obtain reference substance mixture solution;
4. determination of fingerprint spectrum: precisely absorbing the test solution and the reference solution, respectively, injecting into a high performance liquid chromatograph, and measuring to obtain chromatograms of the Notopterygii rhizoma decoction for eliminating dampness and the reference solution;
5. constructing a fingerprint spectrum: importing chromatograms of different batches of notopterygium root herb wet-out soup into traditional Chinese medicine chromatogram fingerprint similarity evaluation software (2012 edition), selecting chromatographic peaks contained in each chromatogram as common peaks, generating notopterygium root wet-out soup comparison fingerprint, calculating relative retention time of each common peak, and evaluating similarity of notopterygium root wet-out soup in each batch.
In the step 1, the weight ratio of notopterygium root, pubescent angelica root, honey-fried licorice root, szechuan lovage rhizome, Chinese ligusticum rhizome, divaricate saposhnikovia root and chastetree fruit is 6: 6: 3: 3: 3: 3: 1.8, the crushing particle size of each medicinal material is coarse powder or coarse powder.
In the step 1, in the comprehensive weighted score, the extract amount accounts for 20%, the extract amount of the cimicifuga root glycosides accounts for 40%, and the extract amount of the ammonium glycyrrhizinate accounts for 40%.
In the step 1, in the orthogonal test, three levels of water addition are set as 1: 10. 1: 15 and 1: three levels of the decoction time were set to 5 minutes, 10 minutes and 15 minutes, and three levels of the number of times of decoction were set to 1 time, 2 times and 3 times, 20.
In the step 1, the optimal decocting process is to add 20 times of water, and to decoct for 3 times, 10 minutes each time.
In the step 2, the volume fraction of the used methanol aqueous solution is 30-80%, and the concentration of the test solution is 2-10 mg/mL.
In the step 3, the volume fraction of the methanol aqueous solution is 30-70%, and the concentration of each standard substance is 50-200 mug/mL.
In the step 4, the chromatographic conditions for establishing the fingerprint of the notopterygium root Shengshi decoction are as follows: the chromatographic column is an Agilent Zorbax extended C18 column (4.6X 250mm, 5 μm); the detection wavelength is 254 nm; the flow rate is 1.0 mL/min; the column temperature was 25 ℃; the sample injection amount is 20 mu L; the mobile phase is 0.1 percent phosphoric acid water (A) and acetonitrile (B) for gradient elution; the gradient elution procedure was: 0-10 min, 15-20% of B; 10-15 min, 20-35% B; 15-25 min, 35-40% B; 25-35 min, 40-65% B; 35-40 min, 65-70% B; 40-45 min, 70-100% B.
In the step 5, the constructed fingerprint of the notopterygium root Shengshi decoction contains 20 common peaks, the peak 10 is taken as a reference peak, and the relative retention time of each common peak is as follows in sequence: peak 1: 0.288-0.292; peak 2: 0.318 to 0.321; peak 3: 0.463 to 0.466; peak 4: 0.496-0.500; peak 5: 0.553-0.559; peak 6: 0.575 to 0.579; peak 7: 0.634-0.637; peak 8: 0.742-0.747; peak 9: 0.874-0.883; peak 10: 1; peak 11: 1.059-1.063; peak 12: 1.112-1.117; peak 13: 1.250-1.255; peak 14: 1.341-1.351; peak 15: 1.382-1.391; peak 16: 1.415 to 1.426; peak 17: 1.468-1.480; peak 18: 1.628-1.636; peak 19: 1.693-1.707; peak 20: 1.757-1.772.
In the step 5, the common peaks include seven characteristic peaks, wherein the peak 2 is linarin, the peak 5 is ferulic acid, the peak 12 is ammonium glycyrrhizinate, the peak 15 is vitexin, the peak 18 is notopterygium alcohol, the peak 19 is osthole, and the peak 20 is isoimperatorin. Ammonium glycyrrhizinate, vitexin at 15, notopterygium alcohol at 18, osthole at 19, isoimperatorin at 20.
The invention has the beneficial effects that:
(1 the preparation method of the notopterygium root decoction for eliminating dampness provided by the invention can obtain higher cream yield and extraction rate of active ingredients in medicinal materials, and the method is stable and reliable.
(2) The HPLC fingerprint spectrum established based on the standard decoction of the notopterygium root decoction can comprehensively reflect the chemical component information and the integral characteristics of the notopterygium root decoction, contains most of pharmacological active components, and provides basis for the quality control of the notopterygium root decoction.
(3) The fingerprint method established by the invention is simple and convenient to operate, short in time consumption, high in accuracy, good in accuracy and good in repeatability, and is easy to realize.
Drawings
FIG. 1 is a chromatogram of a sample of Notopterygii rhizoma decoction for eliminating dampness (1-20: 20 common peaks; 2. linarin glycoside 5. ferulic acid 12. ammonium glycyrrhizinate 15. vitexin 18. notopterygium alcohol 19. cnidium lactone 20. isoimperatorin)
FIG. 2 is a fingerprint overlay of 13 batches of Notopterygii rhizoma decoction for treating rheumatism according to an embodiment of the present invention
Detailed Description
The present invention will be further described with reference to the following examples, but the present invention is not limited to the following embodiments.
Examples
1. Preparation process of notopterygium root decoction for treating rheumatism
Taking the comprehensive weighted score of the extract amount, the extract amount of the Shengma glycoside and the extract amount of the ammonium glycyrrhizinate as evaluation indexes, and adopting L9(34) And (4) performing orthogonal test, namely performing test by taking the water adding amount, the decoction time and the decoction times as investigation factors and performing process verification. The specific implementation process is as follows:
d, drawing a standard curve of the l-ephedrine glycoside and ammonium glycyrrhetate reference substance: accurately weighing appropriate amount of linarin and ammonium glycyrrhizinate as reference substances, adding 70% methanol solution, and making into a series of concentrated solutionsThe solution of the concentration is filtered through a 0.22 mu m microporous filter membrane and injected into a liquid chromatograph. Chromatographic conditions were as follows: the column was an Agilent Zorbax extended C18 column (4.6 × 250mm, 5 μm), the mobile phase was 0.02% phosphoric acid water (a) and acetonitrile (B), and the gradient elution procedure was as follows: 0-10 min, 20-30% of B; 10-25 min, 30-50% of B. The detection wavelength was 254nm, and the flow rate was 1.0mL/min-1The column temperature was 25 ℃ and the amount of sample was 20. mu.L. And (3) drawing a standard curve by taking the peak area (Y) as an ordinate and the concentration (X) as an abscissa: the l-ephedrine glycoside is Y-0.5271X-0.2214 (R)20.9999), ammonium glycyrrhizinate Y0.3067X-4.4822 (R)2＝0.9924)。
The orthogonal test optimization preparation process comprises the following steps: considering water addition amount, decocting time and decocting times as 3 factors, setting 3 levels (see table 1 for factor level) for each factor according to L9 (3)4) Orthogonal experiments were performed. Respectively pulverizing Notopterygii rhizoma and radix Angelicae Pubescentis 6.00g, rhizoma Ligustici, radix Saposhnikoviae, radix Glycyrrhizae Preparata, rhizoma Ligustici Chuanxiong 3.00g, and fructus Vitics Simplicifoliae 1.80g into coarse powder, adding distilled water, decocting with strong fire to boil, maintaining slight boiling with slow fire for a period of time, stopping heating, filtering while hot, and centrifuging; taking 15ml of liquid medicine, freeze-drying to obtain notopterygium root wet-clearing decoction freeze-dried powder, weighing, and calculating the paste amount. Weighing appropriate amount of lyophilized powder of Notopterygii rhizoma decoction, accurately weighing, placing in 10mL volumetric flask, adding 70% methanol water solution for dissolving, ultrasonically dissolving, filtering with 0.22 μm microporous membrane, injecting into liquid chromatograph, calculating the concentration of L-ephedrine glycoside and ammonium glycyrrhizinate according to standard curve, and calculating the extraction amount. Taking the comprehensive weighted score of the cream yield, the extract amount of the L-ephedrine glycoside and the extract amount of the ammonium glycyrrhetate as evaluation indexes, and analyzing the result of the orthogonal test, wherein the comprehensive score is the cream yield (g) multiplied by 0.2+ the L-ephedrine glycoside content (mg) multiplied by 0.4+ the ammonium glycyrrhetate content (mg) multiplied by 0.4.
TABLE 1 factor level design Table
TABLE 2 orthogonal test Table
TABLE 3 analysis of variance
Note: f0.05(2,2)＝19.000
As can be seen from tables 2 and 3, the influence sequence of the three factors on the decoction efficiency of the Notopterygii rhizoma decoction for eliminating dampness is as follows: number of times of decoction>Amount of added water>The optimal decocting process obtained according to the comprehensive grading is A3B2C3The preparation method comprises decocting the above materials in 20 times of water for 3 times each for 10 min.
The best process verification test: decocting according to the optimal decocting process, and performing 3 times of parallel experiments to obtain 11.48g of paste, 9.85mg of L-ephedrine glycoside, 104.16mg of ammonium glycyrrhizinate and 47.90 of comprehensive score, which indicates that the decocting process is stable and reliable and has good repeatability.
2. Establishment of fingerprint of notopterygium root decoction standard decoction
Preparation of a test solution: preparing 13 batches of notopterygium root decoction samples for eliminating dampness according to an optimal decoction process, and freeze-drying the samples into powder. Precisely weighing 41.2mg of freeze-dried powder, placing the powder in a 10mL volumetric flask, adding 70% methanol water solution for dissolving, ultrasonically dissolving, and filtering through a 0.22 mu m microporous filter membrane to obtain the product.
Preparation of control solutions: weighing linarin, ferulic acid, ammonium glycyrrhizinate, vitexin, notopterygium alcohol, cnidium lactone and isoimperatorin reference substances, respectively 1.5mg, adding 70% methanol to constant volume of 10mL, and filtering with 0.22 μm microporous membrane.
Determination of fingerprint spectrum: precisely sucking 13 batches of notopterygium root dampness eliminating soup sample solution and mixed reference solution into a high performance liquid chromatograph, measuring, and recording chromatograms to obtain chromatograms of 13 batches of notopterygium root dampness eliminating soup and mixed reference. The chromatographic conditions are as follows: the chromatographic column is an Agilent Zorbax extended C18 column (4.6X 250mm, 5 μm); the wavelength is 254 nm; the elution flow rate is 1.0 mL/min; the column temperature was 25 ℃; the sample injection amount is 20 mu L; mobile phase elution was gradient with 0.1% phosphoric acid water (a) and acetonitrile (B): 0-10 min, 15-20% of B; 10-15 min, 20-35% B; 15-25 min, 35-40% B; 25-35 min, 40-65% B; 35-40 min, 65-70% B; 40-45 min, 70-100% B.
Constructing a fingerprint spectrum: introducing chromatograms of 13 batches of notopterygium root sheng-shu decoction samples into a traditional Chinese medicine chromatogram fingerprint similarity evaluation system (2012 edition), selecting 20 chromatographic peaks of all batches of samples as common peaks, performing multipoint correction and automatic matching by using an average method to generate a reference chromatogram of the notopterygium root sheng-shu decoction, and respectively showing superposed graphs of the fingerprints of the samples and 13 batches of the notopterygium root sheng-shu decoction in figures 1 and 2. Seven chromatographic peaks are identified by the reference, wherein peak 2 is linarin, peak 5 is ferulic acid, peak 12 is ammonium glycyrrhizinate, peak 15 is vitexin, peak 18 is notopterygium alcohol, peak 19 is osthole, and peak 20 is isoimperatorin. Taking peak 10 as a reference peak, the relative retention time of each common peak is as follows: peak 1: 0.288-0.292; peak 2: 0.318 to 0.321; peak 3: 0.463 to 0.466; peak 4: 0.496-0.500; peak 5: 0.553-0.559; peak 6: 0.575 to 0.579; peak 7: 0.634-0.637; peak 8: 0.742-0.747; peak 9: 0.874-0.883; peak 10: 1; peak 11: 1.059-1.063; peak 12: 1.112-1.117; peak 13: 1.250-1.255; peak 14: 1.341-1.351; peak 15: 1.382-1.391; peak 16: 1.415-1.423; peak 17: 1.468-1.480; peak 18: 1.628-1.636; peak 19: 1.693-1.707; peak 20: 1.757-1.768. The relative retention time RSD of each common peak is between 0.10 and 0.44 percent, which indicates that the peak emergence time of the common peak is more stable.
And (3) similarity evaluation: similarity evaluation is carried out on 13 batches of notopterygium root Shengshi soup by using a traditional Chinese medicine chromatographic fingerprint similarity evaluation system (2012 edition), results are shown in table 4, the similarity is greater than 0.93, and the consistency of 13 batches of samples is better.
Fingerprint similarity evaluation results of Notopterygium incisum decoction of 413 batches in table
Claims (10)
1. A preparation method of a notopterygium root decoction standard decoction for eliminating dampness and a fingerprint spectrum thereof are characterized by comprising the following specific steps:
(1) preparation of standard decoction: respectively pulverizing Notopterygii rhizoma, radix Angelicae Pubescentis, radix Glycyrrhizae Preparata, rhizoma Ligustici Chuanxiong, rhizoma Ligustici, radix Saposhnikoviae, and fructus Vitics Simplicifoliae into certain particle size, mixing at a certain ratio, adding distilled water with certain volume, soaking for half an hour, boiling with strong fire, decocting with slow fire for a certain time, filtering when it is hot after the decoction is finished, and centrifuging to obtain standard decoction; taking the comprehensive weighted score of the paste yield, the rubusoside extraction yield and the ammonium glycyrrhizinate extraction yield as evaluation indexes, adopting an orthogonal test to investigate the influence of three factors of water addition, decoction time and decoction times on the decoction process, obtaining an optimal decoction process, and carrying out process verification;
(2) preparing a fingerprint sample solution: respectively decocting Notopterygii rhizoma decoction to obtain standard decoctions, lyophilizing to obtain powder, adding methanol water solution with certain concentration, dissolving to obtain test solution with certain concentration, and coating with membrane;
(3) preparation of control solutions: accurately weighing appropriate amount of linarin, ferulic acid, ammonium glycyrrhizinate, vitexin, notopterygium alcohol, osthole, and isoimperatorin reference substances, adding methanol water solution with certain concentration for dissolving, diluting to 10mL, and filtering with microporous membrane to obtain reference substance mixture solution;
(4) determination of fingerprint spectrum: precisely absorbing the test solution and the reference solution, respectively, injecting into a high performance liquid chromatograph, and measuring to obtain chromatograms of the Notopterygii rhizoma decoction for eliminating dampness and the reference solution;
(5) constructing a fingerprint spectrum: importing chromatograms of different batches of notopterygium root moisture-eliminating decoction into traditional Chinese medicine chromatogram fingerprint similarity evaluation software (2012 version), selecting chromatographic peaks contained in the chromatograms of the batches as common peaks, generating notopterygium root moisture-eliminating decoction comparison fingerprint, calculating relative peak area and relative retention time of the common peaks, and evaluating the similarity of the notopterygium root moisture-eliminating decoction in the batches.
2. The preparation method of the notopterygium root decoction for treating rheumatism and the fingerprint thereof as claimed in claim 1, wherein in the step (1), the weight ratio of notopterygium root, pubescent angelica root, honey-fried licorice root, chuanxiong rhizome, ligusticum, ledebouriella root and chastetree fruit is 6: 6: 3: 3: 3: 3: 1.8, the crushing particle size of each medicinal material is coarse powder or coarse powder.
3. The method for preparing Notopterygii rhizoma decoction for eliminating dampness and its fingerprint as claimed in claim 1, wherein in said step (1), the extract amount accounts for 20%, the extract amount of Shengma glycoside accounts for 40%, and the extract amount of ammonium glycyrrhizinate accounts for 40%.
4. The preparation method of the Notopterygium incisum decoction for eliminating dampness and the fingerprint thereof as claimed in claim 1, wherein in the step (1), three levels of water addition amount are set as 1: 10. 1: 15 and 1: three levels of the decoction time were set to 5 minutes, 10 minutes and 15 minutes, and three levels of the number of times of decoction were set to 1 time, 2 times and 3 times, 20.
5. The preparation method of the notopterygium root decoction for eliminating dampness and the fingerprint thereof as claimed in claim 1, wherein in the step (1), the optimal decocting process is to add 20 times of water and to decoct for 3 times, 10 minutes each time.
6. The preparation method of the Notopterygium incisum decoction for eliminating dampness and the fingerprint thereof as claimed in claim 1, wherein in the step (2), the volume fraction of the methanol aqueous solution is 30% -80%, and the concentration of the test solution is 2-10 mg/mL.
7. The preparation method of the notopterygium root decoction for eliminating dampness standard decoction and the fingerprint thereof as claimed in claim 1, wherein in the step (3), the volume fraction of the methanol aqueous solution is 30% -70%, and the concentration of each standard is 50-200 μ g/mL.
8. The preparation method of the notopterygium root decoction for eliminating dampness standard decoction and the fingerprint thereof according to claim 1 are characterized in that in the step (4), the chromatographic conditions for establishing the notopterygium root decoction for eliminating dampness fingerprint are as follows: the chromatographic column is an Agilent Zorbax extended C18 column (4.6X 250mm, 5 μm); the detection wavelength is 254 nm; the flow rate is 1.0 mL/min; the column temperature was 25 ℃; the sample injection amount is 20 mu L; the mobile phase is 0.1 percent phosphoric acid water (A) and acetonitrile (B) for gradient elution; the gradient elution procedure was: 0-10 min, 15-20% of B; 10-15 min, 20-35% B; 15-25 min, 35-40% B; 25-35 min, 40-65% B; 35-40 min, 65-70% B; 40-45 min, 70-100% B.
9. The preparation method of the Notopterygium incisum decoction and the fingerprint thereof according to claim 1, wherein the constructed Notopterygium incisum decoction fingerprint in the step (5) contains 20 common peaks, taking peak 10 as a reference peak, and the relative retention time of each common peak is as follows: peak 1: 0.288-0.292; peak 2: 0.318 to 0.321; peak 3: 0.463 to 0.466; peak 4: 0.496-0.500; peak 5: 0.553-0.559; peak 6: 0.575 to 0.579; peak 7: 0.634-0.637; peak 8: 0.742-0.747; peak 9: 0.874-0.883; peak 10: 1; peak 11: 1.059-1.063; peak 12: 1.112-1.117; peak 13: 1.250-1.255; peak 14: 1.341-1.351; peak 15: 1.382-1.391; peak 16: 1.415 to 1.426; peak 17: 1.468-1.480; peak 18: 1.628-1.636; peak 19: 1.693-1.707; peak 20: 1.757-1.772.
10. The method for preparing the Notopterygii rhizoma decoction for eliminating dampness and the fingerprint thereof as claimed in claim 1, wherein in the step (5), the common peaks include seven characteristic peaks, wherein peak 2 is linalooside, peak 5 is ferulic acid, peak 12 is ammonium glycyrrhizinate, peak 15 is vitexin, peak 18 is Notopterygii rhizoma alcohol, peak 19 is osthole, and peak 20 is isoimperatorin.
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