CN114921321A - Microbial fermentation equipment for propionic acid production and propionic acid fermentation process - Google Patents

Microbial fermentation equipment for propionic acid production and propionic acid fermentation process Download PDF

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CN114921321A
CN114921321A CN202210610514.3A CN202210610514A CN114921321A CN 114921321 A CN114921321 A CN 114921321A CN 202210610514 A CN202210610514 A CN 202210610514A CN 114921321 A CN114921321 A CN 114921321A
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fermentation
propionic acid
liquid
fixedly connected
microorganisms
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魏伟国
魏伟清
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Shenzhen Xiameng Biotechnology Co ltd
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Shenzhen Xiameng Biotechnology Co ltd
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    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/02Membranes; Filters
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    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02Stirrer or mobile mixing elements
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/16Vibrating; Shaking; Tilting
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    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/08Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by vibration
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/40Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/52Propionic acid; Butyric acids

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Abstract

The invention relates to the field of propionic acid production, and particularly relates to a microbial fermentation device for propionic acid production and a propionic acid fermentation process. The technical problem that the production efficiency of propionic acid is influenced because aged microorganisms not only occupy partial area of membrane materials but also have the production and fermentation efficiency far lower than that of normal microorganisms in the propionic acid fermentation process is solved. The invention provides a microbial fermentation device for propionic acid production, which comprises a lifting unit, a mixing vibration component and the like; the lifting unit is connected with the mixed vibration component. According to the invention, the bacteria carrying membrane carrying microorganisms is arranged into a spiral structure, so that not only is continuous multiplication of the microorganisms facilitated, but also replacement of the bacteria carrying membrane is facilitated, the aged microorganisms on the bacteria carrying membrane are easy to shake off from the surface of the bacteria carrying membrane and are discharged along with flowing liquid in the fermentation cabin, a multiplication space is provided for normal microorganisms on the bacteria carrying membrane, the acid production efficiency of flora on the bacteria carrying membrane is improved, and finally, detection liquid collected in the collection cylinder flows out through the electromagnetic valve at the lower end.

Description

Microbial fermentation equipment for propionic acid production and propionic acid fermentation process
Technical Field
The invention relates to the field of propionic acid production, and particularly relates to a microbial fermentation device for propionic acid production and a propionic acid fermentation process.
Background
In the process of producing propionic acid by using a biological fermentation technology, Chinese patent CN106244661B describes a method for producing propionic acid by fermenting a mixed bacteria system, and the production method controls the distribution of microorganisms in a fermentation device by regulating the concentration of ammonium ions, so that the fermentation environment is favorable for the proliferation of propionic acid-producing microorganisms, thereby producing high-concentration and high-purity propionic acid.
The production process effectively utilizes microorganisms to improve the production efficiency of propionic acid fermentation, however, as the microorganisms in the fermentation equipment are loaded on the membrane material, and the membrane material is fixed in the fermentation equipment in a discontinuous mode, the proliferation range of the microorganisms on the membrane material in different areas is effective, in addition, as the microorganisms are in the continuous proliferation period, even in the microorganisms with symmetrical division, the components among the sub-flora can have the phenomenon of differential distribution, the microorganisms with the possibility of generating senescence can be generated, the aged microorganisms not only occupy the membrane material in partial area, but also have the production and fermentation efficiency far lower than that of the normal microorganisms, thereby influencing the overall production efficiency of the propionic acid fermentation.
Disclosure of Invention
In order to overcome the defects that in a propionic acid fermentation process, aged microorganisms not only occupy partial area of membrane materials, but also have the production and fermentation efficiency far lower than that of normal microorganisms, so that the overall production efficiency of propionic acid fermentation is influenced, the invention provides a microbial fermentation device for propionic acid production and a propionic acid fermentation process.
The technical scheme is as follows: a microbial fermentation device for propionic acid production comprises a lifting unit, a mixing vibration assembly, a rotary supporting unit, a main support, a fermentation cabin, a top cover, a liquid inlet pipe, a liquid outlet pipe, a collecting cylinder and a bacteria-carrying membrane; the interior of the main bracket is fixedly connected with a fermentation cabin through a flange; the top of the fermentation cabin is fixedly connected with a top cover through a flange; the lower side of the fermentation cabin is communicated with a liquid inlet pipe for filling liquid; the upper side of the fermentation chamber is communicated with a liquid outlet pipe which overflows liquid; the middle part of the fermentation cabin is fixedly connected with a collecting cylinder through an upper fixing frame and a lower fixing frame; the left side and the right side of the collecting cylinder are respectively provided with a vertical strip-shaped through groove structure; the interior of the collecting cylinder is connected with a lifting unit; the upper side of the lifting unit is connected with a top cover; a rotary supporting unit is connected between the upper fixing frame and the lower fixing frame; the rotary supporting unit is connected with the fermentation cabin; when the lifting unit drives the mixed vibration component to move upwards, the mixed vibration component vibrates and rotates a plurality of bacteria-carrying membranes on the supporting unit, so that the bacteria-carrying membranes in a spiral structure shake off aged microorganisms, and liquid in all areas in the fermentation chamber is uniformly mixed; during the lifting unit drives the mixed vibration assembly to move downwards, the mixed vibration assembly controls the lifting unit to seal the through groove structures on two sides of the collecting cylinder, so that the detection liquid collected in the collecting cylinder flows out through the electromagnetic valve at the lower end.
More preferably, the liquid inlet pipe is communicated with a ring pipe at the inner bottom of the fermentation chamber, and the ring pipe is positioned below all the bacterial membranes.
More preferably, a plurality of through hole structures are arranged on the upper surface of the surrounding annular pipe.
More preferably, the lifting unit comprises a motor, a screw rod, a first turntable, a baffle and a locking block; the upper side of the top cover is fixedly connected with a motor; a screw rod is rotatably connected inside the collecting cylinder; the screw rod is connected with the rotary supporting unit; an output shaft of the motor is fixedly connected with a screw rod; the upper end and the lower end of the collecting cylinder are respectively connected with a first rotating disc in a rotating way; a baffle is fixedly connected between the front sides and the rear sides of the two first turntables respectively; the right sides of the two baffles are respectively provided with a plurality of slot structures; the screw rod and the two baffles are both connected with the mixed vibration assembly; a plurality of locking blocks are fixedly connected to the inner right side of the collecting cylinder; the front side and the left side of each locking block are respectively provided with a lock head structure; the left lock head structure of each locking block is respectively inserted with a slot structure of a baffle.
More preferably, the mixing vibration assembly comprises a central slide block, a side slide block, a second rotary disc, a paddle, a liquid guide pipe and a liquid spray pipe; the inner sides of the two baffles are respectively provided with a sliding chute structure; the sliding groove structures of the two baffles are respectively connected with a side sliding block in a sliding way; a central sliding block is fixedly connected between the two side sliding blocks through bolts; the lower side of the central sliding block is rotatably connected with a second rotary table; a plurality of blades are fixedly connected around the outer surface of the second turntable; a plurality of liquid guide pipes are fixedly connected around the outer edge of the second rotating disc; the lower end of each liquid guide pipe is communicated with a liquid spraying pipe with an L-shaped structure.
More preferably, the liquid guiding pipes are all provided with a bucket-shaped structure with a wide upper side and a narrow lower side.
More preferably, the rotary supporting unit comprises a first spur gear, a second spur gear, a third rotary table, a third spur gear, a first mounting frame, an upper mounting rod, a lower mounting rod, a fourth rotary table and a second mounting frame; the upper end of a rotating shaft part of the screw rod is fixedly connected with a first straight gear; the upper side of the fermentation cabin is rotationally connected with a second straight gear through a rotating shaft; the second straight gear is meshed with the first straight gear; the outer surface of the upper fixing frame is rotationally connected with a third turntable; a third straight gear is fixedly connected to the upper side of the third rotary table; the second straight gear is meshed with the third straight gear; a plurality of first mounting frames are fixedly connected around the outer surface of the third turntable; the upper side of the lower fixing frame is rotatably connected with a fourth turntable; a plurality of second mounting frames are fixedly connected around the outer surface of the fourth turntable; an upper mounting rod is fixedly connected between the upper sides of each first mounting frame and one second mounting frame; a lower mounting rod is fixedly connected between the lower sides of each first mounting frame and one second mounting frame; each upper mounting rod and one lower mounting rod are connected with the same bacterium carrying membrane.
More preferably, each of the upper mounting rods and each of the lower mounting rods are provided in a spiral structure corresponding to the biofilm carrier.
More preferably, the upper end of each bacteria-carrying membrane is provided with two lug structures; the outer side of each first mounting frame is provided with two lug structures; the lug structure of each fungus carrying membrane is respectively inserted with the lug structure of the first mounting frame.
A propionic acid fermentation process comprises the following working steps:
s1: fermentation, the microorganisms are uniformly distributed on the surface of the membrane material with a spiral structure, so that the area of a single membrane material is greatly amplified, and the continuous proliferation of the microorganisms and the quick replacement of the membrane material are facilitated;
s2: the aged microorganisms are removed, the aged microorganisms on the membrane material are shaken off from the surface of the membrane material and are discharged along with flowing liquid, a proliferation space is provided for normal microorganisms on the membrane material, and the acid production efficiency of microbial flora on the membrane material is improved;
s3: stirring, namely uniformly stirring and mixing the liquid in the fermentation equipment, so that the uniformity of components of the culture solution in each area of the fermentation equipment is improved, and the efficient proliferation of microorganisms in the membrane material is facilitated;
s4: sample detection, namely sampling and detecting liquid in the fermentation equipment at regular intervals, and analyzing components of the liquid in the fermentation equipment at different time intervals so as to adjust the components of the culture solution filled into the fermentation equipment in time;
s5: and (3) replacing the membrane material, namely replacing the membrane material in the fermentation equipment at regular intervals, so that the membrane material is ensured to provide a stable carrier for microbial proliferation, and the efficient proceeding of the microbial proliferation is ensured.
Has the beneficial effects that: the technical scheme has the advantages that the bacteria carrying membranes carrying microorganisms are arranged to be of a spiral structure, so that the area of each single bacteria carrying membrane is greatly amplified, each bacteria carrying membrane has continuity, the continuous proliferation of the microorganisms is facilitated, the workload is greatly reduced in the process of replacing the bacteria carrying membranes, the mixed vibration component vibrates each bacteria carrying membrane on the rotary supporting unit during the period that the arranged lifting unit drives the mixed vibration component to move upwards, the vibration is sequentially generated in each area of the bacteria carrying membranes, as the flora proliferation area of the aging microorganisms is far smaller than that of normal microorganisms, the aging microorganisms on the bacteria carrying membranes are easy to shake off from the surfaces of the bacteria carrying membranes and are discharged along with flowing liquid in the fermentation chamber, a proliferation space is reserved for the normal microorganisms on the bacteria carrying membranes, the acid production efficiency of the flora on the bacteria carrying membranes is improved, and meanwhile, the mixed vibration component enables the liquid in each area in the fermentation chamber to be uniformly mixed, during the period that the lifting unit drives the mixed vibration component to move downwards, the mixed vibration component controls the lifting unit to seal the through groove structures on the two sides of the collecting cylinder, so that the detection liquid collected in the collecting cylinder flows out through the electromagnetic valve at the lower end, and the collection effect of liquid samples in all areas in the fermentation cabin is improved; thereby solving the problems that the aged microorganisms occupy the area of the membrane material and influence the overall production efficiency of the propionic acid fermentation in the propionic acid fermentation process.
Drawings
FIG. 1 is a schematic perspective view of the present application;
FIG. 2 is a cross-sectional view of the fermentation tank and roof of the present application;
fig. 3 is a partial perspective view of the lifting unit and the rotation supporting unit of the present application;
fig. 4 is a partial perspective view of the lifting unit of the present application;
FIG. 5 is a perspective view of the baffle of the present application;
fig. 6 is a schematic perspective view of a lock block of the present application;
fig. 7 is a partial perspective view of the lifting unit and the hybrid vibration module of the present application;
FIG. 8 is a perspective view of the hybrid shock assembly of the present application;
FIG. 9 is a perspective view of a catheter and a spray tube according to the present application;
FIG. 10 is a first partial perspective view of the rotary support unit of the present application;
fig. 11 is a second partial perspective view of the rotation support unit of the present application.
Wherein the figures include the following reference numerals: 1-main support, 2-fermentation chamber, 21-upper fixed mount, 22-lower fixed mount, 3-top cover, 4-liquid inlet pipe, 41-annular pipe, 411-through hole, 5-liquid outlet pipe, 6-collection cylinder, 61-through groove, 62-electromagnetic valve, 7-bacteria carrying membrane, 71-lug, 101-motor, 102-screw rod, 103-first rotary disc, 104-baffle, 1041-slot, 1042-sliding groove, 105-locking block, 1051-lock head, 201-central sliding block, 202-side sliding block, 203-second rotary disc, 204-paddle, 205-liquid guide pipe, 206-liquid spray pipe, 301-first straight gear, 302-second straight gear, 303-third rotary disc, 304-third straight gear, 305-first mounting rack, 3051-lug, 306-upper mounting bar, 307-lower mounting bar, 308-fourth turntable, 309-second mounting frame.
Detailed Description
Embodiments of the present invention will be described below with reference to the drawings.
A propionic acid fermentation process comprises the following working steps:
s1: fermentation, the microorganisms are uniformly distributed on the surface of the membrane material with a spiral structure, so that the area of a single membrane material is greatly amplified, and the continuous proliferation of the microorganisms and the quick replacement of the membrane material are facilitated;
s2: the aging microorganisms are removed, the aging microorganisms on the membrane material are shaken off from the surface of the membrane material and are discharged along with flowing liquid, a proliferation space is provided for normal microorganisms on the membrane material, and the acid production efficiency of microbial flora on the membrane material is improved;
s3: stirring, namely uniformly stirring and mixing the liquid in the fermentation equipment, so that the uniformity of components of the culture solution in each area of the fermentation equipment is improved, and the efficient proliferation of microorganisms in the membrane material is facilitated;
s4: sample detection, namely sampling and detecting liquid in the fermentation equipment at regular intervals, and analyzing components of the liquid in the fermentation equipment at different time intervals so as to adjust the components of the culture solution filled into the fermentation equipment in time;
s5: and (3) replacing the membrane material, namely replacing the membrane material in the fermentation equipment at regular intervals, so that the membrane material is ensured to provide a stable carrier for microbial proliferation, and the efficient proceeding of the microbial proliferation is ensured.
Examples
A microorganism fermentation device for propionic acid production is shown in figures 1-11, and comprises a lifting unit, a mixing vibration component, a rotary supporting unit, a main support 1, a fermentation cabin 2, a top cover 3, a liquid inlet pipe 4, a liquid outlet pipe 5, a collecting cylinder 6 and a bacteria-carrying membrane 7; the interior of the main bracket 1 is connected with a fermentation cabin 2 through a flange bolt; the top of the fermentation cabin 2 is connected with a top cover 3 through a flange bolt; the lower side of the fermentation cabin 2 is communicated with a liquid inlet pipe 4; the upper side of the fermentation chamber 2 is communicated with a liquid outlet pipe 5; the middle part of the fermentation cabin 2 is fixedly connected with a collecting cylinder 6 through an upper fixing frame 21 and a lower fixing frame 22; the left side and the right side of the collecting cylinder 6 are respectively provided with a vertical strip-shaped through groove 61 structure; the interior of the collecting cylinder 6 is connected with a lifting unit; the upper side of the lifting unit is connected with a top cover 3; the lower side of the lifting unit is connected with a mixed vibration component; a rotary supporting unit is connected between the upper fixing frame 21 and the lower fixing frame 22; the rotary supporting unit is connected with the fermentation cabin 2; the rotary supporting unit is connected with the lifting unit; the rotary supporting unit is connected with a plurality of bacteria-carrying membranes 7 with spiral structures.
As shown in fig. 2, at the bottom of the fermentation chamber 2, the liquid inlet pipe 4 is communicated with an annular pipe 41, and the annular pipe 41 is positioned below all the bacterial membranes 7; a plurality of through holes 411 are arranged around the upper surface of the annular tube 41.
As shown in fig. 3-6, the lifting unit comprises a motor 101, a screw rod 102, a first turntable 103, a baffle plate 104 and a locking block 105; the upper side of the top cover 3 is connected with a motor 101 through bolts; a screw rod 102 is rotatably connected inside the collecting cylinder 6; the screw rod 102 is connected with the rotary supporting unit; an output shaft of the motor 101 is fixedly connected with a screw rod 102; the upper end and the lower end of the collecting cylinder 6 are respectively connected with a first rotary disc 103 in a rotating way; a baffle plate 104 is welded between the front side and the rear side of each of the two first turntables 103; the right sides of the two baffles 104 are respectively provided with a plurality of slot 1041 structures; the screw rod 102 and the two baffles 104 are connected with the mixed vibration component; a plurality of locking blocks 105 are fixedly connected to the inner right side of the collecting cylinder 6; the front side and the left side of each locking block 105 are respectively provided with a lock head 1051 structure; the left locking head 1051 structure of each locking piece 105 is inserted into a slot 1041 structure of the baffle plate 104.
As shown in fig. 7-9, the mixing and vibrating assembly comprises a central slide 201, a side slide 202, a second rotating disk 203, a paddle 204, a liquid guide tube 205 and a liquid spraying tube 206; the inner sides of the two baffles 104 are respectively provided with a chute 1042 structure; the sliding grooves 1042 of the two baffles 104 are respectively connected with a side sliding block 202 in a sliding way; a central slider 201 is fixedly connected between the two side sliders 202 through bolts; the lower side of the central sliding block 201 is rotatably connected with a second turntable 203; a plurality of paddles 204 are welded around the outer surface of the second rotating disc 203; a plurality of liquid guide pipes 205 are fixedly connected around the outer edge of the second rotating disc 203; the liquid guide pipes 205 are all arranged into a bucket-shaped structure with a wide upper side and a narrow lower side; the lower end of each liquid guide pipe 205 is communicated with a liquid spray pipe 206 with an L-shaped structure.
As shown in fig. 3, 10 and 11, the rotation support unit includes a first spur gear 301, a second spur gear 302, a third rotary table 303, a third spur gear 304, a first mounting bracket 305, an upper mounting rod 306, a lower mounting rod 307, a fourth rotary table 308 and a second mounting bracket 309; a first straight gear 301 is fixedly connected to the upper end of a rotating shaft part of the screw rod 102; the upper side of the fermentation cabin 2 is rotationally connected with a second straight gear 302 through a rotating shaft; the second spur gear 302 is meshed with the first spur gear 301; the outer surface of the upper fixing frame 21 is rotationally connected with a third turntable 303; a third spur gear 304 is fixedly connected to the upper side of the third rotating disc 303; the second spur gear 302 is meshed with the third spur gear 304; a plurality of first mounting brackets 305 are connected around the outer surface of the third turntable 303 through bolts; the upper side of the lower fixing frame 22 is rotatably connected with a fourth rotating disc 308; a plurality of second mounting brackets 309 are bolted around the outer surface of the fourth turntable 308; an upper mounting rod 306 is fixedly connected between the upper sides of each first mounting rack 305 and one second mounting rack 309; a lower mounting rod 307 is fixedly connected between the lower sides of each first mounting rack 305 and one second mounting rack 309; each upper mounting rod 306 is connected with one lower mounting rod 307 to form the same bacteria-carrying membrane 7; each upper mounting rod 306 and each lower mounting rod 307 are arranged in a spiral structure corresponding to the bacteria-carrying membrane 7; the upper end of each bacteria-carrying membrane 7 is provided with two lug plate 71 structures; the outer side of each first mounting frame 305 is provided with two lug 3051 structures; the lug 71 structure of each mycoderm 7 is inserted with the lug 3051 structure of the first mounting rack 305.
The outer surface of the bacteria carrying membrane 7 is distributed with microbial flora, an external pump machine injects culture solution into the fermentation chamber 2 through the liquid inlet pipe 4, the culture solution uniformly and upwards gushes from the through hole 411 of the annular pipe 41, so that the fermentation chamber 2 is filled with culture solution for helping microorganism proliferation, the microbial flora on the surface of the bacteria carrying membrane 7 continuously proliferates, the surface of the bacteria carrying membrane 7 is fully covered, simultaneously propionic acid generated by microorganism fermentation is dissolved into the culture solution, and overflows into external separation equipment along with the culture solution in the fermentation chamber 2 from the liquid outlet pipe 5, the fermentation and separation work of the propionic acid is continuously carried out, and the efficient and high-quality production of the propionic acid is ensured.
With the proliferation of microbial flora on the surface of the bacteria-carrying membrane 7, part of aged microorganisms in the microbial flora need to be removed from the aged microorganisms with low propionic acid fermentation efficiency on the surface of the bacteria-carrying membrane 7, firstly, the output shaft of the motor 101 drives the screw rod 102 to rotate in the forward direction, the screw rod 102 drives the central slider 201 and the side sliders 202 connected thereto to rise upwards along the sliding groove 1042 of the baffle 104, the central slider 201 drives the second rotary disk 203 and the paddles 204, the liquid guide tube 205 and the liquid spray tube 206 connected thereto to move upwards, the liquid above the liquid guide tube 205 is captured by the liquid guide tube 205 moving upwards and is sprayed out from the liquid spray tube 206 to the periphery, meanwhile, the paddles 204 moving upwards are blocked by the liquid to drive the second rotary disk 203 to rotate along the central slider 201, the liquid guide tube 205 and the liquid spray tube 206 are driven by the second rotary disk 203 to diffuse the sprayed out fluid to all directions, so that the fluid sprayed out from the liquid spray tube 206 is sprayed out to all areas on the surface of the bacteria-carrying membrane 7 from bottom to top in turn, the bacterium carrying membrane 7 is vibrated, so that the bacterium carrying membrane 7 is vibrated from bottom to top, and as the flora multiplication area of the aging microorganisms is far smaller than that of the normal microorganisms, the aging microorganisms on the bacterium carrying membrane 7 are easy to shake off from the surface of the bacterium carrying membrane 7 and are discharged along with flowing liquid in the fermentation chamber 2, so that a multiplication space is provided for the normal microorganisms on the bacterium carrying membrane 7, and the acid production efficiency of the flora on the bacterium carrying membrane 7 is improved.
During the period that the screw rod 102 drives the central slider 201 and the second turntable 203 to rise upwards, the screw rod 102 drives the first straight gear 301 to rotate, the first straight gear 301 is meshed with the second straight gear 302 and drives the second straight gear 302 to rotate, the second straight gear 302 is meshed with the third straight gear 304 and drives the third turntable 303 to rotate, the third turntable 303 drives the upper mounting rod 306 and the lower mounting rod 307 on the first mounting rack 305, and the second mounting rack 309 and the fourth turntable 308 connected with the upper mounting rod 306 and the lower mounting rod 307 rotate around the axis of the screw rod 102, so that the upper mounting rod 306 and the lower mounting rod 307 drive each area of the bacteria-carrying membrane 7 to sequentially pass through the through groove 61 of the collecting cylinder 6, each area of the bacteria-carrying membrane 7 is sequentially vibrated by the fluid sprayed by the liquid spraying pipe 206 to perform the vibration removal work on the aged microorganisms, and simultaneously, the rotating paddle 204 performs the stirring work on the liquid in the fermentation cabin 2 from bottom to top, thereby improving the uniformity of each area component of the culture liquid in the fermentation cabin 2, is beneficial to the high-efficiency proliferation of the microorganisms in the bacteria-carrying membrane 7.
After the central slider 201 moves to the top of the collecting cylinder 6, the sampling operation of the liquid in the fermentation chamber 2 is started, firstly, the output shaft of the motor 101 drives the screw rod 102 to rotate reversely, the screw rod 102 drives the central slider 201 and the side slider 202 connected thereto, the baffle 104 is pushed to drive the first rotary disk 103 to rotate counterclockwise at an overlooking angle, the slot 1041 of the rear baffle 104 is separated from the left locking head 1051 of the locking block 105, until the baffle 104 at the front side rotates ninety degrees, the slot 1041 is locked on the outer surface of the front locking head 1051 of the locking block 105, at this time, the two baffles 104 respectively seal the through slots 61 at the left and right sides of the collecting cylinder 6, so that the collecting cylinder 6 forms a sealed pipeline, then, the output shaft of the motor 101 continues to drive the screw rod 102 to rotate, the baffle 104 is blocked by the locking block 105 and cannot rotate, so that the screw rod 102 drives the central slider 201 and the side slider 202 connected thereto to reset downwards along the sliding slot 1042 of the baffle 104, simultaneously the paddle 204 that moves down receives the blockking of liquid and drives second carousel 203 and rotate along central slider 201, homogenizes the processing by the liquid of paddle 204 from the top down collection in the collection cylinder 6, later solenoid valve 62 opens, and the liquid of collecting in the collection cylinder 6 flows through solenoid valve 62 downwards to in advancing the external analytical equipment of solenoid valve 62, carry out the composition analysis to the liquid of current period in fermentation cabin 2, in order in time to adjust the culture solution composition of pouring into in fermentation cabin 2.
After completing the fermentation work of a period, the fungus carrying membrane 7 needs to be replaced, after most of the liquid in the fermentation cabin 2 is evacuated through the liquid inlet pipe 4, the lug 71 at the upper end of the fungus carrying membrane 7 is structurally pulled out from the lug 3051, meanwhile, the lead screw 102 drives the first straight gear 301 to rotate, so that the rotating rotary supporting unit can tear the fungus carrying membrane 7 from the upper mounting rod 306 and the lower mounting rod 307 in one step in the rotating process, and then the new fungus carrying membrane 7 is attached to the upper mounting rod 306 and the lower mounting rod 307 according to the steps, so that the fungus carrying membrane 7 can be replaced quickly, the operation is simple and convenient, and the influence on the production of propionic acid is small.
The present application is described in detail above, and specific examples are applied herein to explain the principles and embodiments of the present application, and the descriptions of the above examples are only used to help understand the method and the core idea of the present application; meanwhile, for a person skilled in the art, according to the idea of the present application, there may be variations in the specific embodiments and the application scope, and in summary, the content of the present specification should not be construed as a limitation to the present application.

Claims (10)

1. A microbial fermentation device for propionic acid production comprises a main support (1), a fermentation chamber (2), a top cover (3), a liquid inlet pipe (4) and a liquid outlet pipe (5); a fermentation cabin (2) is fixedly connected inside the main bracket (1) through a flange; the top of the fermentation cabin (2) is fixedly connected with a top cover (3) through a flange; the lower side of the fermentation chamber (2) is communicated with a liquid inlet pipe (4) filled with liquid; the upper side of the fermentation chamber (2) is communicated with a liquid outlet pipe (5) which overflows liquid; the device is characterized by also comprising a lifting unit, a mixed vibration component, a rotary supporting unit, a collecting cylinder (6) and a bacteria-carrying membrane (7); the middle part of the fermentation cabin (2) is fixedly connected with a collecting cylinder (6) through an upper fixing frame (21) and a lower fixing frame (22); the left side and the right side of the collecting cylinder (6) are respectively provided with a vertical strip-shaped through groove (61) structure; the interior of the collecting cylinder (6) is connected with a lifting unit; the upper side of the lifting unit is connected with a top cover (3); a rotary supporting unit is connected between the upper fixing frame (21) and the lower fixing frame (22); the rotary supporting unit is connected with the fermentation cabin (2); during the period that the lifting unit drives the mixed vibration component to move upwards, the mixed vibration component vibrates and rotates a plurality of bacteria carrying membranes (7) on the supporting unit, so that the bacteria carrying membranes (7) in a spiral structure shake off the aged microorganisms, and liquid in each area in the fermentation cabin (2) is uniformly mixed; during the lifting unit drives the mixed vibration component to move downwards, the mixed vibration component controls the lifting unit to seal the through groove (61) structures on two sides of the collecting cylinder (6), so that the detection liquid collected in the collecting cylinder (6) flows out through the electromagnetic valve (62) at the lower end.
2. The microbial fermentation equipment for propionic acid production according to claim 1, wherein the liquid inlet pipe (4) is communicated with an annular pipe (41) at the inner bottom of the fermentation chamber (2), and the annular pipe (41) is positioned below all the bacterial membranes (7).
3. The microbial fermentation equipment for propionic acid production of claim 2, wherein the upper surface of the annular pipe (41) is provided with a plurality of through holes (411).
4. The microbial fermentation equipment for propionic acid production according to claim 1, wherein the lifting unit comprises a motor (101), a screw rod (102), a first rotating disc (103), a baffle plate (104) and a locking block (105); the upper side of the top cover (3) is fixedly connected with a motor (101); a screw rod (102) is rotatably connected inside the collecting cylinder (6); the screw rod (102) is connected with the rotary supporting unit; an output shaft of the motor (101) is fixedly connected with a screw rod (102); the upper end and the lower end of the collecting cylinder (6) are respectively and rotatably connected with a first rotating disc (103); a baffle (104) is fixedly connected between the front side and the rear side of each of the two first turntables (103); the right sides of the two baffles (104) are respectively provided with a plurality of slot (1041) structures; the screw rod (102) and the two baffles (104) are connected with the mixed vibration assembly; a plurality of locking blocks (105) are fixedly connected to the inner right side of the collecting cylinder (6); the front side and the left side of each locking block (105) are respectively provided with a lock head (1051) structure; the structure of the left lock head (1051) of each lock block (105) is respectively inserted with the structure of the slot (1041) of one baffle plate (104).
5. The microbial fermentation equipment for propionic acid production of claim 4, wherein the mixing and vibrating component comprises a central slide block (201), a side slide block (202), a second rotary disc (203), a paddle (204), a liquid guide pipe (205) and a liquid spraying pipe (206); the inner sides of the two baffles (104) are respectively provided with a sliding chute (1042) structure; the sliding grooves (1042) of the two baffles (104) are respectively connected with a side sliding block (202) in a sliding way; a central sliding block (201) is fixedly connected between the two side sliding blocks (202) through bolts; a second turntable (203) is rotatably connected to the lower side of the central slider (201); a plurality of paddles (204) are fixedly connected around the outer surface of the second rotating disc (203); a plurality of liquid guide pipes (205) are fixedly connected around the outer edge of the second rotating disc (203); the lower end of each liquid guide pipe (205) is communicated with a liquid spraying pipe (206) with an L-shaped structure.
6. The fermentation apparatus of claim 5, wherein the liquid guide pipes (205) are each configured as a bucket-shaped structure with a wide upper side and a narrow lower side.
7. The microbial fermentation equipment for propionic acid production of claim 4, wherein the rotary supporting unit comprises a first straight gear (301), a second straight gear (302), a third rotary disc (303), a third straight gear (304), a first mounting frame (305), an upper mounting rod (306), a lower mounting rod (307), a fourth rotary disc (308) and a second mounting frame (309); a first straight gear (301) is fixedly connected to the upper end of a rotating shaft part of the screw rod (102); the upper side of the fermentation cabin (2) is rotationally connected with a second straight gear (302) through a rotating shaft; the second straight gear (302) is meshed with the first straight gear (301); the outer surface of the upper fixing frame (21) is rotationally connected with a third turntable (303); a third spur gear (304) is fixedly connected to the upper side of the third rotating disc (303); the second spur gear (302) is meshed with the third spur gear (304); a plurality of first mounting frames (305) are fixedly connected around the outer surface of the third turntable (303); the upper side of the lower fixing frame (22) is rotationally connected with a fourth rotating disc (308); a plurality of second mounting racks (309) are fixedly connected around the outer surface of the fourth turntable (308); an upper mounting rod (306) is fixedly connected between the upper sides of each first mounting frame (305) and one second mounting frame (309); a lower mounting rod (307) is fixedly connected between the lower sides of each first mounting frame (305) and one second mounting frame (309); each upper mounting rod (306) is connected with a lower mounting rod (307) to the same bacteria-carrying membrane (7).
8. The apparatus of claim 7, wherein each of the upper mounting rods (306) and each of the lower mounting rods (307) are arranged in a spiral structure corresponding to the carrier film (7).
9. The microbial fermentation equipment for propionic acid production is characterized in that the upper end of each bacteria-carrying membrane (7) is provided with two lug (71) structures; the outer side of each first mounting rack (305) is provided with two lug boss (3051) structures; the lug (71) structure of each bacteria-carrying membrane (7) is inserted with the lug (3051) structure of the first mounting rack (305).
10. A propionic acid fermentation process is characterized in that: the processing technology uses a microorganism fermentation device for producing propionic acid, which is disclosed by any one of claims 1 to 9 and comprises the following working steps:
s1: fermentation, microorganisms are uniformly distributed on the surface of the membrane material with a spiral structure, so that the area of a single membrane material is greatly amplified, and the continuous proliferation of the microorganisms and the quick replacement of the membrane material are facilitated;
s2: the aging microorganisms are removed, the aging microorganisms on the membrane material are shaken off from the surface of the membrane material and are discharged along with flowing liquid, a proliferation space is provided for normal microorganisms on the membrane material, and the acid production efficiency of microbial flora on the membrane material is improved;
s3: stirring, namely uniformly stirring and mixing the liquid in the fermentation equipment, so that the uniformity of components of the culture solution in each area of the fermentation equipment is improved, and the efficient proliferation of microorganisms in the membrane material is facilitated;
s4: sample detection, namely sampling and detecting liquid in the fermentation equipment at regular intervals, and analyzing components of the liquid in the fermentation equipment at different time intervals so as to adjust the components of the culture solution filled into the fermentation equipment in time;
s5: and (3) replacing the membrane material, namely replacing the membrane material in the fermentation equipment at regular intervals, so that the membrane material is ensured to provide a stable carrier for microbial proliferation, and the efficient proceeding of the microbial proliferation is ensured.
CN202210610514.3A 2022-05-31 2022-05-31 Microbial fermentation equipment for propionic acid production and propionic acid fermentation process Pending CN114921321A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202210610514.3A CN114921321A (en) 2022-05-31 2022-05-31 Microbial fermentation equipment for propionic acid production and propionic acid fermentation process

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202210610514.3A CN114921321A (en) 2022-05-31 2022-05-31 Microbial fermentation equipment for propionic acid production and propionic acid fermentation process

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CN114921321A true CN114921321A (en) 2022-08-19

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116376669A (en) * 2023-04-04 2023-07-04 江苏润普食品科技股份有限公司 Self-cleaning type fermentation device for producing food-grade propionic acid

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116376669A (en) * 2023-04-04 2023-07-04 江苏润普食品科技股份有限公司 Self-cleaning type fermentation device for producing food-grade propionic acid
CN116376669B (en) * 2023-04-04 2023-10-17 江苏润普食品科技股份有限公司 Self-cleaning type fermentation device for producing food-grade propionic acid

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