CN114916564A - Essential oil composition for inhibiting novel coronavirus - Google Patents

Essential oil composition for inhibiting novel coronavirus Download PDF

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CN114916564A
CN114916564A CN202210587685.9A CN202210587685A CN114916564A CN 114916564 A CN114916564 A CN 114916564A CN 202210587685 A CN202210587685 A CN 202210587685A CN 114916564 A CN114916564 A CN 114916564A
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essential oil
protein
inhibiting
novel coronavirus
beta
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唐健元
赵梓亦
谢春光
万李娜
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Teaching Hospital of Chengdu University of TCM
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    • A—HUMAN NECESSITIES
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    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A01N65/22—Lamiaceae or Labiatae [Mint family], e.g. thyme, rosemary, skullcap, selfheal, lavender, perilla, pennyroyal, peppermint or spearmint
    • A—HUMAN NECESSITIES
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    • A01N65/08—Magnoliopsida [dicotyledons]
    • A01N65/36—Rutaceae [Rue family], e.g. lime, orange, lemon, corktree or pricklyash
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    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
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Abstract

The application discloses an essential oil composition for inhibiting novel coronavirus, which consists of not less than 40Wt% of lemon essential oil, 18-25Wt% of lavender essential oil, 15-18Wt% of pogostemon cablin essential oil and the balance of peppermint essential oil, wherein the limonene content in the lemon essential oil is higher than 30Wt%, the sabinene content is higher than 14 Wt%, and the citronellal content is higher than 10 Wt%. The essential oil composition provided by the invention can be rapidly combined with the S protein on the surface of the novel coronavirus, so that the S protein of the novel coronavirus loses the opportunity of being combined with the ACE-2 protein on the surface of the receptor protein, and meanwhile, the essential oil carries out epitope modification on the S protein, changes the information transmission mode of the S protein, so that the virus cannot be retested, and the effect of inhibiting and killing the virus is realized.

Description

Essential oil composition for inhibiting novel coronavirus
Technical Field
The invention relates to the field of essential oil compositions, in particular to an antibacterial, bacteriostatic and antiviral plant essential oil composition, and specifically relates to an essential oil composition for inhibiting novel coronavirus.
Background
Bacteria and viruses are the main pathogens that infect humans and animals, but the ways of protection and killing differ based on their structural differences. Bacteria are unicellular organisms with intact cell walls, cell membranes, cytoplasm and nucleoplasm, whereas viruses belong to the class of non-cellular organisms with only internal nucleic acids (DNA or RNA), the protein shell that surrounds them.
In the viral classification, coronaviruses belong phylogenetically to the genus coronaviruses (Coronaviridae) of the order Nidovirales (Nidovirales) coronaviruses (Coronavirus). Viruses of the genus coronaviruses are enveloped (envelope) RNA viruses with a linear single-stranded positive strand genome, and are a large group of viruses that are widely found in nature.
2019 novel coronavirus (2019-nCoV, named after the world health organization in 2020, 1 month; SARS-CoV-2, named after the international committee for viral classification in 2020, 2 month, 11 months). Coronaviruses are a large family of viruses known to cause the common cold and more serious diseases such as Middle East Respiratory Syndrome (MERS) and Severe Acute Respiratory Syndrome (SARS). The epidemic situation caused by new coronary pneumonia has covered the world for over 3 years and is repeated and continuous in different countries and regions, although vaccines aiming at new coronary viruses are developed at present, because the virus strains have strong variability and rapid mutation, the vaccines aiming at the early new coronary viruses are not enough to completely protect the mutated viruses, so in daily protection, in order to avoid the diseases caused by the inhalation of the viruses into respiratory tracts, the viruses need to be filtered and inhibited in a protection stage so as to achieve effective protection and reduce or avoid the occurrence of infection.
Disclosure of Invention
In order to reduce the infection risk of viruses, in particular to reduce the infection risk problem of coronaviruses, such as new coronaviruses, on the respiratory tract of a human body, the essential oil composition for inhibiting the new coronaviruses is provided, and the essential oil composition is coated on a position for protecting or ventilating air, so that S protein on the surface of the new tubular viruses can be effectively combined with substances volatilized from the essential oil composition, the replication of the viruses is inhibited or destroyed, and the viruses are harmless. The principle is that the S protein (Spike protein) on the surface of the novel coronavirus can be combined with ACE-2 protein on the surface of receptor protein, so as to infect the receptor, cause the receptor to develop diseases and produce related symptoms; however, the essential oil volatile matter has excellent binding force with the S protein, and when the essential oil volatile matter is combined with the S protein, the essential oil volatile matter is blocked from being combined with the ACE-2 protein, so that the purpose of preventing infection is achieved; further, the essential oil volatile matter changes or modifies the epitope, namely changes the S protein structure, so that the information transmission mode of the virus is changed, and self-replication can not be realized, thereby blocking the possibility of infection, achieving the effect of effectively inhibiting the infection of the novel coronavirus, and ensuring that the probability of the infection of the receptor is greatly reduced and even the virus can not be infected as long as the virus contacts with the set essential oil volatile matter in the air flowing process from the breathing process and is inhaled into the body of the receptor and enters the respiratory epithelium of the receptor even if the current protective measures are not enough to block all viruses, so that the virus loses the replication capacity and is even killed.
In order to achieve the purpose, the technical scheme adopted by the application is as follows:
an essential oil composition for inhibiting new coronavirus comprises lemon essential oil not less than 40Wt%, lavender essential oil 18-25Wt%, herba Agastaches essential oil 15-18Wt%, and Mentha piperita essential oil in balance, wherein the limonene content is higher than 30Wt%, sabinene content is higher than 14 Wt%, and citronellal content is higher than 10 Wt%.
Limonene is a medium for inhibiting inflammation, can effectively accord with trend effect of leucocytes, simultaneously has the capability of interacting with 5-lipoxygenase, TNF-alpha and IL-6, and achieves the antibacterial and antiviral effects. One of the effective components aiming at the positive inhibition principle of the novel coronavirus is citronellal which is proved by demonstration and can generate an effective competitive mechanism in the process of combining SARS-CoV-2 and a receptor ACE-2 protein, namely, the effective component can greatly reduce the chance of combining the receptor ACE-2 protein and the S protein of SARS-CoV-2 by inhibiting the activity of the receptor ACE-2 protein, so that the S protein of SARS-CoV-2 is preferentially combined with citronellal under the same condition, thereby the epitope of the S protein is modified, and the effective self-replication can not be realized. In the present application, all essential oils including lemon essential oil, lavender essential oil, patchouli essential oil and peppermint essential oil which are referred to herein are plant extracts of natural origin, however, as the essential oil attribute content which has been disclosed in the present application, those skilled in the art can also find other natural or artificial essential oils with similar attribute and/or efficacy to the essential oil which has been disclosed in the present application, such as plant essential oils including atractylodes essential oil, bay leaf essential oil and artemisia apiacea essential oil which can be added to the present application individually or in combination, the effectiveness of the addition of other essential oils depends on the degree of coincidence between the added single essential oil or the effective components of the essential oil composition and the present application, and the plant essential oil has a plurality of effective components which can be effectively extracted from different plant essential oils, therefore, the reason for the antiviral effect of the essential oils is not the whole essential oil composition, but rather on the content of its active ingredients. Therefore, the preparation of the main effective components by using the other essential oils in equivalent proportion within the reasonable range of the application is the known suggestion given by the application and does not represent the originality; for this reason, the possible combinations are not listed individually in the present application.
As one of the synergy modes, the lemon essential oil also preferably contains 1Wt% of alpha-thujene, 1.2 Wt% of alpha-pinene, 5-8 Wt% of beta-pinene, 1.3-2 Wt% of (E) -beta-ocimene, 0.1-0.2 Wt% of beta-caryophyllene, 0.2Wt% of gamma-terpineol, 0.2Wt% of delta-elemene and 2.5-4 Wt% of geranial. alpha-Pinene (alpha-Pinene) is an organic compound with the chemical formula of C 10 H 16 The essential oil composition is a colorless transparent liquid, is slightly soluble in water, is insoluble in propylene glycol and glycerol, is soluble in most organic solvents such as ethanol, diethyl ether, chloroform, glacial acetic acid and the like, is an important raw material for synthesizing spices, is mainly used for synthesizing terpineol, linalool and some sandalwood spices, can be used for perfuming daily chemicals and other industrial products, and can be used as a synthetic lubricant and a plasticizer, and can play an additional role in blending smell when being used in an essential oil composition, such as when the essential oil composition is used in protective articles and needs to be directly inhaled into a human body, the more natural and fresh smell is beneficial to improving the practicability. beta-Caryophyllene, also known as beta-Caryophyllene, having a molecular weight of 204.36, is known by the common name beta-CaryophylleneThe liquid is colorless to micro-butter liquid and has light clove-like fragrance. The boiling point is 119-121 ℃ (1466 Pa). Dissolved in ether and ethanol (1 mL in 6mL of 95% ethanol) and insoluble in water. The natural product is contained in essential oils such as oleum Caryophylli, clove stem oil, Cinnamomum zeylanicum bark oil, oleum Cinnamomi, oleum Lavandula Angustifolia, thyme oil, pepper oil, and Zanthoxylum piperitum oil. Therefore, when beta-caryophyllene is required to be obtained, the beta-caryophyllene can be obtained through a wide range of materials. Meanwhile, the gamma-terpineol is one isomer of terpineol, and also comprises alpha-terpineol and beta-terpineol with similar performances.
Preferably, the lavender essential oil contains 5-11Wt% of geraniol, 1-3Wt% of 4-terpineol or terpin-4-ol, 1-4Wt% of 1, 8-cineole, 4-12Wt% of monoterpene linalool, 2-6Wt% of linalyl acetate and 0.5-4Wt% of borneol. The lavender essential oil extracted naturally contains dozens of components, but not all the components of the lavender essential oil can generate a binding reaction with a novel tubular virus and have affinity, the active components of the lavender essential oil are analyzed one by one through a plasma resonance technology (SPR) to determine the affinity of the S protein and human angiotensin converting enzyme 2 (ACE-2), and the components of the lavender have obvious effect on preventing or reducing the affinity of the S protein and human angiotensin converting enzyme 2 (ACE-2) from the determination result.
In the same way, the applicant invention team also performs effectiveness verification on the corresponding components of the following essential oils for preventing or reducing the affinity of the S protein and human angiotensin-converting enzyme 2 (ACE-2), and learns that various effective components exist in the patchouli essential oil and the peppermint essential oil and can effectively form competitive inhibition. The method specifically comprises the following steps: the patchouli essential oil contains 15-25Wt% of patchouli alcohol, 5-7Wt% of alpha-guaiene, 8-10Wt% of beta-guaiene and no less than 5Wt% of eicosene.
Preferably, the essential oil of peppermint comprises 3-6Wt% menthol, 2-4Wt% menthone, 0.3-1Wt% menthyl acetate, 9-12Wt% 1, 8-eucalyptol and not less than 15Wt% menthofuran.
Has the advantages that:
the essential oil composition provided by the invention can be rapidly combined with the S protein on the surface of the novel coronavirus, so that the S protein of the novel coronavirus loses the opportunity of being combined with the ACE-2 protein on the surface of the receptor protein, and meanwhile, the essential oil carries out epitope modification on the S protein, changes the information transmission mode of the S protein, so that the virus cannot be retested, and the effect of inhibiting and killing the virus is realized.
Drawings
FIG. 1 is a graph of the binding affinity of the S protein of SARS-CoV-2 virus to ACE-2 in example 8.
Detailed Description
To make the objects, technical solutions and advantages of the embodiments of the present application clearer, the embodiments of the present application will be clearly and completely described below, and it is obvious that the described embodiments are a part of the embodiments of the present application, but not all of the embodiments of the present application.
Example 1:
an essential oil composition for inhibiting new coronavirus comprises lemon essential oil not less than 40Wt%, lavender essential oil 18-25Wt%, herba Agastaches essential oil 15-18Wt%, and Mentha piperita essential oil in balance, wherein the limonene content is higher than 30Wt%, sabinene content is higher than 14 Wt%, and citronellal content is higher than 10 Wt%.
The limonene is a medium for inhibiting inflammation, can effectively accord with the trend effect of white blood cells, simultaneously has the capability of interacting with 5-lipoxygenase, TNF-alpha and IL-6, and achieves the antibacterial and antiviral effects. One of the effective components aiming at the positive inhibition principle of the novel coronavirus is citronellal which is obtained through demonstration, the effective component can generate an effective competitive mechanism in the process of combining SARS-CoV-2 and a receptor ACE-2 protein, namely, the opportunity of combining the receptor ACE-2 protein and the S protein of SARS-CoV-2 is greatly reduced by inhibiting the activity of the receptor ACE-2 protein, so that the S protein of SARS-CoV-2 is preferentially combined with citronellal under the same condition, the epitope of the S protein is modified, and the effective self-replication cannot be realized. In the present application, all essential oils including lemon essential oil, lavender essential oil, patchouli essential oil and peppermint essential oil referred to herein are plant extracts of natural origin, however, as the content of the essential oil attributes already disclosed in the present application, those skilled in the art can also find other natural or synthetic essential oils having similar attributes and/or effects to the essential oils already disclosed in the present application, such as plant essential oils including atractylodes essential oil, bay leaf essential oil and artemisia apiacea essential oil, which can be added to the present application individually or in combination, the addition effectiveness of other essential oils depends on the coincidence degree of the added single essential oil or the effective components of the essential oil composition with the present application, and the plant essential oils have multiple effective components extracted from different plant essential oils, therefore, the reason for generating the antiviral effect of the essential oils is not the whole essential oil composition, but rather on the content of its active ingredients. Therefore, the preparation of the main effective components by using the other essential oils in equivalent proportion within the reasonable range of the application is the known suggestion given by the application and does not represent the originality; for this reason, the possible combinations are not listed individually in the present application.
As one of the synergy ways, preferably, the lemon essential oil also contains 1Wt% of alpha-thujene, 1.2 Wt% of alpha-pinene, 5-8 Wt% of beta-pinene, 1.3-2 Wt% of (E) -beta-ocimene, 0.1-0.2 Wt% of beta-caryophyllene, 0.2Wt% of gamma-terpineol, 0.2Wt% of delta-elemene and 2.5-4 Wt% of geranial. alpha-Pinene (alpha-Pinene) is an organic compound with the chemical formula of C 10 H 16 The essence is colorless transparent liquid, slightly soluble in water, insoluble in propylene glycol and glycerin, soluble in most organic solvents such as ethanol, diethyl ether, chloroform, glacial acetic acid and the like, is an important raw material for synthesizing spices, is mainly used for synthesizing terpineol, linalool and some sandalwood type spices, can be used for perfuming daily chemicals and other industrial products, and can be used as a synthetic lubricant and a plasticizer, and the synthetic lubricant and the plasticizer can play an additional role in blending smell when being used in an essential oil composition, for example, when being used in a protective product and needing to be directly inhaled into a human body, the more natural and fresh smell can help to improve the practicability. beta-Caryophyllene, also called beta-Caryophyllene olein, with the common name beta-caryophylelene, molecular weight 204.36, and its character is colorless to micro butterLiquid-like, with a light clove-like aroma. The boiling point is 119-121 ℃ (1466 Pa). Dissolved in ether and ethanol (1 mL in 6mL of 95% ethanol) and insoluble in water. The natural product is contained in essential oils such as oleum Caryophylli, clove stem oil, Cinnamomum zeylanicum bark oil, oleum Cinnamomi, oleum Lavandula Angustifolia, thyme oil, pepper oil, and Zanthoxylum piperitum oil. Therefore, when beta-caryophyllene is required to be obtained, the beta-caryophyllene can be obtained through a wide range of materials. Meanwhile, the gamma-terpineol is one isomer of terpineol, and also comprises alpha-terpineol and beta-terpineol with similar performances.
In this embodiment, the lavender essential oil contains 5-11Wt% of geraniol, 1-3Wt% of 4-terpineol or terpin-4-ol, 1-4Wt% of 1, 8-cineole, 4-12Wt% of monoterpene linalool, 2-6Wt% of linalyl acetate, and 0.5-4Wt% of borneol. The ingredients of the naturally extracted lavender essential oil are very complex, the ingredients are dozens of larger, but not all the ingredients of the lavender essential oil can generate a binding reaction with a novel tubular virus, the ingredients have affinity, the active ingredients of the lavender essential oil are analyzed one by one through a plasma resonance technology (SPR), the affinity of the S protein and human angiotensin converting enzyme 2 (ACE-2) is determined, and the ingredients of the lavender have an obvious effect on preventing or reducing the affinity of the S protein and the human angiotensin converting enzyme 2 (ACE-2) according to the determination result.
In the same way, the applicant invention team also carries out effectiveness verification on the corresponding components of the following essential oils for preventing or reducing the affinity of the S protein and human angiotensin converting enzyme 2 (ACE-2), and learns that a plurality of effective components exist in the patchouli essential oil and the peppermint essential oil and can effectively form competitive inhibition. The method specifically comprises the following steps: the patchouli essential oil contains 15-25Wt% of patchouli alcohol, 5-7Wt% of alpha-guaiene, 8-10Wt% of beta-guaiene and not less than 5Wt% of eicosene. The peppermint essential oil comprises 3-6Wt% of menthol, 2-4Wt% of menthone, 0.3-1Wt% of menthyl acetate, 9-12Wt% of 1, 8-cineole and not less than 15Wt% of menthofuran.
Example 2:
an essential oil composition for inhibiting new coronavirus comprises 47Wt% of lemon essential oil, 18Wt% of lavender essential oil, 15Wt% of pogostemon cablin essential oil, and the balance of peppermint essential oil, wherein the lemon essential oil contains 35Wt% of limonene, 15Wt% of sabinene, and 11Wt% of citronellal.
Example 3:
an essential oil composition for inhibiting new coronavirus comprises lemon essential oil 40Wt%, lavender essential oil 25Wt%, herba Agastaches essential oil 18Wt%, and Mentha piperita essential oil in balance, wherein the lemon essential oil contains limonene 31Wt%, sabinene 15Wt%, and citronellal 20 Wt%.
Example 4:
an essential oil composition for inhibiting a novel coronavirus is composed of 40Wt% of lemon essential oil, 25Wt% of lavender essential oil, 18Wt% of pogostemon cablin essential oil and the balance of peppermint essential oil, wherein the lemon essential oil contains 31Wt% of limonene, 15Wt% of sabinene, 20 Wt% of citronellal, 1Wt% of alpha-thujene, 1.2 Wt% of alpha-pinene, 5Wt% of beta-pinene, 1.3 Wt% of (E) -beta-ocimene, 0.1 Wt% of beta-caryophyllene, 0.2Wt% of gamma-terpineol, 0.2Wt% of delta-elemene and 2.5 Wt% of geranial.
Example 5:
an essential oil composition for inhibiting novel coronavirus is composed of lemon essential oil 40Wt%, lavender essential oil 25Wt%, patchouli essential oil 18Wt%, peppermint essential oil in balance, wherein limonene accounts for 31Wt%, sabinene accounts for 15Wt%, citronellal accounts for 20 Wt%, alpha-thujene 1Wt%, alpha-pinene 1.2 Wt%, beta-pinene 8Wt%, (E) -beta-ocimene 2Wt%, beta-caryophyllene 0.2Wt%, gamma-terpineol 0.2Wt%, delta-elemene 0.2Wt%, and geranial 4 Wt%.
Example 6:
an essential oil composition for inhibiting novel coronavirus comprises 40Wt% of lemon essential oil, 25Wt% of lavender essential oil, 18Wt% of pogostemon cablin essential oil and the balance of peppermint essential oil, wherein the lavender essential oil comprises 5-11Wt% of geraniol, 1-3Wt% of 4-terpineol or terpineol-4-ol, 1-4Wt% of 1, 8-cineole, 4-12Wt% of monoterpene linalool, 2-6Wt% of linalyl acetate and 0.5-4Wt% of borneol.
Example 7:
an essential oil composition for inhibiting a novel coronavirus comprises 40Wt% of lemon essential oil, 25Wt% of lavender essential oil, 18Wt% of pogostemon cablin essential oil and the balance of peppermint essential oil, wherein the lavender essential oil comprises 5-11Wt% of geraniol, 1-3Wt% of 4-terpineol or terpineol-4-ol, 1-4Wt% of 1, 8-cineole, 4-12Wt% of monoterpene linalool, 2-6Wt% of linalyl acetate and 0.5-4Wt% of borneol, and the pogostemon cablin essential oil comprises 15-25Wt% of pogostenol, 5-7Wt% of alpha-guaiene, 8-10Wt% of beta-guaiene and not less than 5Wt% of eicosene.
Example 8:
preparation of essential oil dilution sample: an equal amount of the essential oil composition of example 1-example 7 was taken, and a sample with a concentration of 35mol/L was labeled as sample 1-7, and the samples 1-7 were diluted at a ratio of 1:80 and emulsified conventionally to obtain an equal amount of emulsified essential oil diluted samples 1-7 for use.
And (3) affinity determination: the affinity of the S (Spike protein) protein of SARS-CoV-2 virus and human angiotensin converting enzyme 2 (ACE-2) is determined by Surface Plasmon Resonance (SPR), the SARS-COV-2 pseudovirus stock solution used in this example was purchased from Nanjing Kingsry Biotech, Inc., and the receptor solution containing human angiotensin converting enzyme 2 (ACE-2) was prepared by the laboratory of the research center of the applicant and derived from the oral and nasal mucosa of human body.
The receptor solution containing human angiotensin converting enzyme 2 (ACE-2) is placed on the surface of the sensor chip, SARS-CoV-2 sample solution with the concentration of 1200nM and the equal volume of distilled water mixture are injected, and the natural affinity curve A is obtained after 600s of reaction.
The receptor solution containing human angiotensin converting enzyme 2 (ACE-2) is placed on the surface of a sensor chip, SARS-CoV-2 sample solution with the concentration of 1200nM and a 75% medical alcohol mixture with the same volume are injected, and a reverse affinity curve B is obtained after reaction for 600 s.
Placing a receptor solution containing human angiotensin converting enzyme 2 (ACE-2) on the surface of a sensor chip, injecting a SARS-CoV-2 sample solution with the concentration of 1200nM and an essential oil diluted sample 1-7 with the same volume, respectively mixing to obtain a mixture, and uniformly reacting for 600s to obtain an affinity curve C-curve I after the essential oil diluted sample 1-7 is added, which is shown in detail in figure 1.
As can be seen from FIG. 1, the binding affinity of the S (Spike protein) protein of SARS-CoV-2 virus and angiotensin-converting enzyme 2 (ACE-2) is very high without the intervention of other substances, as shown in curve A, the maximum resonance signal (RU) is more than 110, but after the sample is diluted by adding 75% medical alcohol and any oil of the invention, the resonance signal (RU) is greatly reduced to below 15, as shown in curve B; it can be seen that the addition of 75% medical alcohol can kill SARS-CoV-2 virus, and because of the problems of mixing time and uniformity, the virus can not be completely killed in a short time, and the SARS-CoV-2 which is not in contact with 75% medical alcohol in time can be continuously combined with human angiotensin converting enzyme 2 (ACE-2) in the solution, so that a very low resonance signal (RU) can be detected.
As can be seen from the graph A and the graph B in FIG. 1, the higher the resonance signal (RU) is at the same concentration, the stronger the binding affinity of the S (Spike protein) protein of SARS-CoV-2 virus to human angiotensin-converting enzyme 2 (ACE-2) is. After the essential oil diluted samples 1-7 are respectively added, because the mixture ratio of the essential oil diluted samples 1-7 is different, the components and the content are different, and the extreme value of the resonance signal (RU) of the generated resonance curve C-I is not uniform, and the reason is caused by the change of the content of the effective component capable of being combined with the S protein to cause the change of the concentration difference, but as can be seen from figure 1, the resonance signal (RU) of the curve C-I is always between 45-80, and is higher than the resonance signal value of the medical alcohol participating effect of 75 percent, but is lower than the resonance signal value of the samples 1-7 without the essential oil diluted samples, which shows that the SARS-CoV-2 virus has good inhibition under the effect of the essential oil with the current concentration.
Thereby verifying the conclusion that the binding affinity of SARS-CoV-2 virus S protein and human angiotensin converting enzyme 2 (ACE-2) is obviously inhibited and hindered, and the reason for the rapid decrease of the binding affinity of SARS-CoV-2 virus S protein and human angiotensin converting enzyme 2 (ACE-2) is two: one is that the S protein epitope is effectively modified, so that effective combination and replication can not be carried out, and pathogenicity is lost; the second is that S protein has been killed and dies. Based on the above two possibilities, both of which are the expected results of the present invention, the goal of inhibiting the transmission/infection of new coronaviruses to new receptors has been fully achieved. The curve result of the Surface Plasmon Resonance (SPR) experiment is enough to confirm that the essential oil provided by the invention contains specific components and active ingredients in a content range, so that the binding affinity of SARS-CoV-2 virus S protein and human angiotensin converting enzyme 2 (ACE-2) can be effectively reduced, the infection capacity of SARS-CoV-2 on human bodies is reduced, and the effect of inhibiting or even killing SARS-CoV-2 is achieved.
It is worth to be noted that, in the qualitative judgment of the Surface Plasmon Resonance (SPR) experiment by adding the above essential oil, only the essential oil composition provided by the present invention has strong inhibitory effect on the binding affinity of the SARS-CoV-2S protein and human angiotensin converting enzyme 2 (ACE-2), but whether the SARS-CoV-2 can be effectively killed and the efficiency, time and proportion of killing can be effectively verified, and the applicant has not performed quantitative demonstration yet.
The above description is only a preferred embodiment of the present application and is not intended to limit the present application, and various modifications and changes may be made to the present application by those skilled in the art. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims (5)

1. An essential oil composition for inhibiting a novel coronavirus, comprising: the lemon essential oil comprises, by weight, not less than 40% of lemon essential oil, 18-25% of lavender essential oil, 15-18% of pogostemon cablin essential oil and the balance of peppermint essential oil, wherein the limonene content is higher than 30% by weight, the sabinene content is higher than 14% by weight and the citronellal content is higher than 10% by weight.
2. An essential oil composition for inhibiting a novel coronavirus according to claim 1, wherein: the lemon essential oil also comprises 1Wt% of alpha-thujene, 1.2 Wt% of alpha-pinene, 5-8 Wt% of beta-pinene, 1.3-2 Wt% of (E) -beta-ocimene, 0.1-0.2 Wt% of beta-caryophyllene, 0.2Wt% of gamma-terpineol, 0.2Wt% of delta-elemene and 2.5-4 Wt% of geranial.
3. An essential oil composition for inhibiting a novel coronavirus according to claim 1 or 2, wherein: the lavender essential oil contains 5-11Wt% of geraniol, 1-3Wt% of 4-terpineol or terpineol-4-ol, 1-4Wt% of 1, 8-cineole, 4-12Wt% of monoterpene linalool, 2-6Wt% of linalyl acetate and 0.5-4Wt% of borneol.
4. An essential oil composition for inhibiting a novel coronavirus according to claim 3, wherein: the patchouli essential oil contains 15-25Wt% of patchouli alcohol, 5-7Wt% of alpha-guaiene, 8-10Wt% of beta-guaiene and no less than 5Wt% of eicosene.
5. An essential oil composition for inhibiting a novel coronavirus according to claim 4, wherein: the peppermint essential oil comprises 3-6Wt% of menthol, 2-4Wt% of menthone, 0.3-1Wt% of menthyl acetate, 9-12Wt% of 1, 8-cineole and not less than 15Wt% of menthofuran.
CN202210587685.9A 2022-05-27 2022-05-27 Essential oil composition for inhibiting novel coronavirus Withdrawn CN114916564A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115839522A (en) * 2022-11-01 2023-03-24 光谷洁净(武汉)科技有限公司 High-efficient low resistance air cleaner
CN116058388A (en) * 2023-02-01 2023-05-05 徐宝华 Composition capable of sterilizing and inhibiting viruses and application thereof

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115839522A (en) * 2022-11-01 2023-03-24 光谷洁净(武汉)科技有限公司 High-efficient low resistance air cleaner
CN115839522B (en) * 2022-11-01 2023-08-25 光谷洁净(武汉)科技有限公司 High-efficient low resistance air cleaner
CN116058388A (en) * 2023-02-01 2023-05-05 徐宝华 Composition capable of sterilizing and inhibiting viruses and application thereof

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Application publication date: 20220819