CN114916496B - Method for detecting mouse cooperative behavior - Google Patents
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Abstract
The application provides a detection method of mouse cooperative behavior, which comprises a food limiting stage, a training stage and a cooperative behavior detection stage; feeding the experimental mice in a single cage, feeding the experimental mice without limiting water, recording the weight of the experimental mice every day, recording the weight of the experimental mice, dividing the experimental mice into a group A and a group B at random, respectively performing independent training and mastering the behavior test rules, and in a cooperative behavior detection stage, after the independent training stage, taking one experimental mouse from the group A and one experimental mouse from the group B, respectively pairing the experimental mice, starting detection of two different cooperative modes after the experimental mice are adapted to each other, and observing the mastering degree of the mice on the test rules and the cooperative behaviors among the experimental mice by counting the task success rate of the mice and the latency of each time of completing the tasks. The method realizes automatic tracking and automatic rewarding of the mouse cooperation test, detects the cooperation of multiple modes of the mouse, and provides support for exploring corresponding nerve loop mechanisms according to the mouse cooperation.
Description
Technical Field
The application belongs to the technical field of collaborative behavior pattern research based on mice, and particularly relates to a detection method of mouse collaborative behaviors.
Background
In nature, cooperation is a necessary choice adopted by species in the face of natural selection for achieving the purposes of survival development, reproduction, co-evolution and the like, and is specifically represented by social winter protection, natural enemies co-defense, nest co-building, combined hunting, even solving some complex problems and the like. In social life, collaboration refers to a kind of cooperative action between individuals and groups that cooperate with each other for the purpose of common purpose. Reports indicate that social communication disorders are commonly manifested when humans or animals are in certain disease states such as autism, depression, anxiety, social phobia, etc., severely affecting inter-individual communication, and thus exhibiting less or more difficult mutual assistance in a collaborative setting. The theory of group selection, theory of genetic selection, theory of reciprocity, and social punishment are mainly described in the mechanism of cooperative behavior, but no hypothesis is known to fully explain all the mechanisms behind the theory, and the key is that the corresponding neural loop mechanism of cooperative behavior is still very little known. Rodents are the most commonly used animal model in current behavioural neurobiological research. However, most of the current mouse behavioural researches are based on the task and detection of a single mouse, and the lack of more than two mouse cooperative detection methods brings a lot of inconveniences to the cooperative behavior researches and also influences the exploration of related diseases.
Disclosure of Invention
In order to solve the problems in the prior art, the application provides a mouse cooperative behavior detection device and method, which can quickly and simply study the cooperative behavior of a mouse in a behavior pattern and provide support for exploring a corresponding nerve loop mechanism according to the mouse cooperative behavior.
In order to achieve the above purpose, the technical scheme adopted by the application is as follows: a detection method of mouse cooperative behavior comprises a food limiting stage, a training stage and a cooperative behavior detection stage; feeding the experimental mice in a single cage in a food limiting stage, wherein the food limiting is not limited to water, recording the weight of the experimental mice every day and controlling the weight of the experimental mice to be 80-90% of a baseline;
in the training stage, experimental mice are randomly divided into a group A and a group B, and are respectively trained independently and mastered with behavior test rules, and the training stage comprises three steps: step one, adapting and mastering rules for triggering rewards by experimental mice; secondly, training the experimental mice repeatedly for a plurality of times every day by introducing specific rules to ensure that the experimental mice master the rules step by step; thirdly, strengthening the mastering of rules by the experimental mice by continuously shortening the duration of each test;
and in the cooperative behavior detection stage, after the independent training stage, taking and pairing one experimental mouse of the group A and one experimental mouse of the group B, and starting detection of two different cooperative modes after the two experimental mice are adapted to each other: one is that one mouse completes the task and the other mouse gets rewarded within a prescribed test time; after one mouse finishes the task, the other mouse finishes the task within the specified test time, and both sides obtain rewards, otherwise, both sides cannot obtain rewards; the mastery degree of the mice on the test rules and the mutual cooperative behavior are observed by counting the success rate of the tasks of the mice and the latency of completing the tasks each time.
The device comprises an experiment box body and a control system, wherein the top of the experiment box body is provided with a camera system and an acousto-optic system, the camera system is connected with the input end of the control system, and the control signal input end of the acousto-optic system is connected with the output end of the control system; the side surface of the experiment box body is provided with a reward device, the side surface of the experiment box body is semitransparent, and the control signal input end of the reward device is connected with the output end of the control system; the first observation area and the second observation area which are symmetrically distributed are marked on the bottom surface of the box body, the first observation area and the second observation area are arranged at the opposite angles of the bottom surface of the box body, and the output port of the rewarding device extends into the experimental box body.
The side plate of the experiment box body is made of semitransparent acrylic plates, and a side door is arranged on the experiment box body.
The rewarding device adopts an automatic feeder controlled by TTL signals, an output port of the automatic feeder is positioned in a first observation area and a second observation area, and the first observation area and the second observation area are both sector areas.
The experiment box includes roof, bottom plate and experiment box curb plate, and experiment box curb plate sets up between roof and bottom plate, and experiment box curb plate and bottom plate and roof all can dismantle the connection.
The roof and bottom plate all offer the recess that is used for packing into experimental box curb plate with experimental box curb plate junction, recess and experimental box curb plate interference fit.
During the feeding limiting stage of 3 days to 0 days before the start of the experiment, all experimental mice are fed in a single cage, initial weight is recorded, water is not limited by feeding, a small amount of mouse food and sugar beans are fed in the morning and evening every day, and the weight change of the experimental mice in the whole behavior test process is recorded.
Training phase first phase-establish rewards rules: dividing all experimental mice into A, B groups at random, and defining a sector area with the radius of 8 cm at any angle of the device as a first observation area and a sector area with the same radius at the opposite angle as a second observation area; when the group A mice enter and stay in the first observation area for more than or equal to 3 seconds, giving a sugar bean reward to the second observation area through the reward device, wherein a work prompt is placed in the first observation area; when the mice in the group B enter and stay in the second observation area for more than or equal to 3 seconds, giving a sugar bean reward to the first observation area through a reward device, training for 1 hour every day, wherein the whole training process is accompanied with illumination prompt, and the second observation area is provided with a working prompt different from the first observation area;
training phase second phase-set up task rules: repeating training for two consecutive days every day, wherein the experimental mice need to complete tasks and obtain rewards within a specified time, the stage is to give illumination prompts to the experimental mice at the beginning of each training, when the mice in the group A and the group B enter within 30 seconds and stay in the first observation area and the second observation area continuously for 3 seconds or more respectively, giving one-time sugar bean rewards to the first observation area and the second observation area through a reward device, closing the illumination prompts after the rewards are given through the reward device, and recording that the training is finished and successful once after 20 seconds of interval; if the mice in the group A and the group B do not enter or enter within 30 seconds and continuously stay in the first observation area and the second observation area for less than 3 seconds respectively, no rewarding is put in, illumination prompt is closed, training is finished after 20 seconds, the training is recorded as failure once, and the mastering degree of the mice on the rules can be observed by counting the success rate of tasks and the incubation period for completing the tasks of all times of the mice;
training phase third phase—strengthening task rules: repeating the reinforcement training every day for two continuous days, reducing the task time after the mice master the rules, and finishing the tasks and obtaining rewards in the time of the reinforcement training experiment mice; the step of setting program is that the experimental mice are given illumination prompt when each training is started, the mice in the A group and the B group respectively enter correspondingly within 20 seconds and 10 seconds and continuously stay in the first observation area and the second observation area for more than or equal to 3 seconds, a sugar bean reward is given to the first observation area and the second observation area through a reward device, the illumination prompt is closed after the reward is given through the reward device, and after 20 seconds, the training is finished and the training is recorded as successful one time; if the mice in the group A and the group B do not enter or enter within 20 seconds and 10 seconds respectively and continuously stay in the first observation area and the second observation area for less than 3 seconds, no rewarding is put in, illumination prompts are closed, training is finished after 20 seconds, the training is recorded as failure once, and the mastering degree of the mice on the rules can be observed by counting the success rate of tasks and the incubation period for completing the tasks of all times of the mice.
The method comprises the steps of in a cooperative detection mode I, pairing groups A and B of experimental mice, placing the mice in a new mouse cage to adapt to the mice of the cooperative object for 2 hours, separating the mice by a partition plate with a vent hole in the middle of the new mouse cage, setting the groups A and B of mice in a detection device at the beginning of each training, giving a primary sugar bean rewarding in a second observation area by a reward device if the groups A of mice enter and stay in the first observation area for 3 seconds or more within 10 seconds, or giving the primary sugar bean rewarding in the first observation area by the reward device if the groups B of mice enter and stay in the second observation area for 3 seconds or more, and closing the light rewarding after the rewarding by the reward device; after 20 seconds of interval, training is finished and is recorded as successful training; if the mice in the A group and the B group do not enter, enter the wrong observation area or enter and stay in the correct observation area for less than 3 seconds within 10 seconds, no rewards are put in, the illumination prompt is closed, and after 20 seconds of interval, training is finished and recorded as failure of training.
And a cooperation behavior detection mode II: the method comprises the steps of adjusting a rewarding mode, setting a group A mouse and a group B mouse into a detection device at the same time, immediately giving a lighting prompt to one mouse when the other mouse enters a correct observation area and stays for 3 seconds or longer under the condition that no prompt exists, and giving a sugar bean rewarding to the first observation area and the second observation area through a rewarding device at the same time if the other mouse enters the correct observation area and stays for 3 seconds or longer within 10 seconds; if the mice do not enter, enter the wrong observation area or enter and stay in the correct observation area for less than 3 seconds within 10 seconds, two cooperative mice cannot obtain rewards, and the mastery degree of the mice on the test rules and the cooperative behavior of the mice are observed by counting the task success rate of the mice and the latency of completing the tasks each time.
Compared with the prior art, the application has at least the following beneficial effects:
the method can be used for researching cooperative behaviors of normal mice and abnormal mice with certain disease states such as autism, depression, anxiety, social phobia and the like, and regulating and controlling a nerve loop mechanism of brain areas related to the behaviors; the method can standardize the cooperative behavior training and testing process, reduce variation, and simultaneously is simpler, so that the cooperative behavior can be accurately quantized; the experimental device can realize automatic control, realize automatic tracking of cooperative behavior test, realize automatic rewarding, realize automatic calculation result, save analysis time and improve efficiency;
the cooperation behavior detection method provided by the application can be used for: (1) training the mice to complete cooperative behavior by learning reward rules and cooperation rules; (2) detecting the cooperative behavior of a plurality of modes of the mice, and researching an intra-brain loop regulation mechanism in the cooperative process of the mice on the basis; (3) recording the movement condition, feeding behavior and social behavior of the mice in the experimental device, and evaluating the physiological state of the mice according to the movement condition, feeding behavior and social behavior; (4) researching the behavior of the mice in the cooperative relationship regulated by a method of optical fiber recording, optogenetic or chemical genetic; (5) study abnormal change characteristics of cooperative behavior under relevant mental disease states are studied by evaluating the cooperative behavior of mice; (6) the study evaluates the effect of specific treatment means on improving the behavioural performance of mice models of relevant mental diseases by detecting the behavioural performance of the mice models.
Drawings
FIG. 1 is a schematic diagram of a behavior test of a device according to the present application.
Fig. 2 is a schematic diagram of a cooperative behavior detection mode based on the device of the present application.
FIG. 3 is a flow chart of a behavioral test.
In the figure, a side plate of a 1-experiment box body, a 2-top plate, a 3-bottom plate and a 4-rewarding device.
Detailed Description
The following description of the embodiments of the present application will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are some, but not all embodiments of the application. All other embodiments, which can be made by those skilled in the art based on the embodiments of the application without making any inventive effort, are intended to be within the scope of the application.
Referring to fig. 1, wherein (a) is a group a mouse behavior training pattern diagram based on the device of the present application, and (B) is a group B mouse behavior training pattern diagram based on the device of the present application; the device comprises an experiment box body, a shooting system, an acousto-optic system, a rewarding device 4 and a control system, wherein the shooting system, the acousto-optic system, the rewarding device 4 and the control system are arranged at the top of the experiment box body, the shooting system is connected with the input end of the control system, and the control signal input end of the acousto-optic system is connected with the output end of the control system; the side surface of the experiment box body is provided with a reward device 4, the side surface of the experiment box body is semitransparent, and the control signal input end of the reward device 4 is connected with the output end of the control system; the first observation area and the second observation area which are symmetrically distributed are marked on the bottom surface of the box body, the first observation area and the second observation area are arranged at the opposite angles of the bottom surface of the box body, the output port of the rewarding device 4 extends into the experimental box body, and the side door is arranged on the experimental box body.
The size of the experiment box body is 30cm, 30cm and 40cm, and the experiment box body is made of semitransparent acrylic plates.
The camera system and the sound-light system are controlled by an upper computer, and the rewarding device 4 adopts an automatic feeder controlled by TTL signals. The automatic combination behavior detection can be realized through the control of the upper computer; the experiment box body comprises a top plate 2, a bottom plate 3 and an experiment box body side plate 1, wherein the experiment box body side plate 1 is arranged between the top plate 2 and the bottom plate 3, and the experiment box body side plate 1 is detachably connected with the bottom plate 3 and the top plate 2; the junction of roof 2 and bottom plate 3 and experiment box curb plate 1 all has seted up the recess that is used for packing into experiment box curb plate 1, recess and experiment box curb plate 1 interference fit.
The rewarding device 4 adopts an automatic feeder controlled by TTL signals, an output port of the automatic feeder is positioned in a first observation area and a second observation area, and the first observation area and the second observation area are all sector areas.
Referring to fig. 1 and 2, the behavior test flow is as follows:
and a feeding limiting stage, wherein the experimental mice are fed in a single cage, the feeding is started without limiting water, the weight of the experimental mice is recorded every day, and the weight of the experimental mice is controlled to be 80-90% of a baseline.
In the training stage, experimental mice are randomly divided into A, B groups, and are respectively and independently trained and mastered with behavior test rules. The stage is divided into three steps: step one, adapting and mastering rules for triggering rewards by experimental mice; secondly, training the experimental mice repeatedly for a plurality of times every day by introducing specific rules to ensure that the experimental mice master the rules step by step; and thirdly, strengthening the mastering of the rules by the experimental mice by continuously shortening the duration of each test.
And in the cooperative behavior detection stage, after the independent training stage, taking and pairing one experimental mouse of the group A and one experimental mouse of the group B, and starting detection of two different cooperative modes after the two experimental mice are adapted to each other: one is that one mouse completes the task and the other mouse gets rewarded within a prescribed test time; and the other is that after one mouse finishes the task, the other mouse finishes the task within the specified test time, and both sides obtain rewards, otherwise, both sides cannot obtain rewards.
Referring to fig. 3, specific test steps are as follows:
1. during the feeding limiting period of 3 days to 0 days before the start of the experiment, all experimental mice are fed in a single cage, initial weight is recorded, water is not limited by feeding, a small amount of mouse food and sugar beans are respectively fed in the morning and evening every day, the weight of the mice is controlled to be 80% -90% of a baseline level, and the weight change of the experimental mice in the whole behavior testing process is recorded.
2. Day 1 to day 2 are the first stage of the training phase-establish rewarding rules: all experimental mice were randomly aliquoted into A, B groups, defining a sector of 8 cm radius at any corner of the device as the first observation area and a sector of equal radius at the opposite corner as the second observation area. The stage is set up to program that when group A mice enter and stay in the first observation area (placing work prompts) for 3 seconds or more, the reward device 4 gives a sugar bean reward to the second observation area; when the group B mice enter and stay in the second observation area (the work prompt different from the group A is placed) for 3 seconds or more, the reward device 4 gives a sugar bean reward to the first observation area, the reward is trained for 1 hour every day, the reward acquisition times are not limited, and the whole training process is accompanied by illumination prompts.
3. Day 3 to day 4 are training phase second phase-establish task rules: two consecutive days, training was repeated 50 times per day, and the experimental mice were required to complete the task and obtain rewards within a prescribed time. The program is set in this stage, the experimental mice are given illumination prompts at the beginning of each training, the mice in the A group and the B group enter within 30 seconds and stay in the first observation area and the second observation area for 3 seconds or more continuously, the reward device 4 gives a sugar bean reward to the second observation area and the first observation area, the illumination prompts are closed after the reward device 4 gives the reward, and after 20 seconds, the training is finished and the training is recorded as successful one time; if the mice in the A group and the B group do not enter or enter within 30 seconds and stay in the first observation area and the second observation area continuously for less than 3 seconds, no rewards are put in, the illumination prompts are closed, and after 20 seconds of interval, training is finished and the training is recorded as failure. The mastery degree of the mice on the rules can be observed by counting the success rate of 50 tasks and the incubation period for completing the tasks of the mice.
4. Day 5 to day 6 are training phase third phase—reinforcement task rules: the reinforcement training was repeated 50 times daily for two consecutive days. The time length of the task is shortened after the mice master the rules, and the training experiment mice are strengthened to finish the task and obtain rewards in a gradually shortened time. The program is set in this stage, the experimental mice are given illumination prompts at the beginning of each training, the mice in the A group and the B group enter within 20 seconds and 10 seconds and stay in the first observation area and the second observation area for more than or equal to 3 seconds, the reward device 4 gives a sugar bean reward in the second observation area and the first observation area, the illumination prompts are closed after the reward device 4 puts the reward, and after 20 seconds, the training is finished and the training is recorded as successful one time; if the mice in the A group and the B group do not enter or enter within 20 and 10 seconds and stay in the first observation area and the second observation area continuously for less than 3 seconds, no rewards are put in, the illumination prompt is closed, and after 20 seconds, training is finished and the training is recorded as failure. The mastery degree of the mice on the rules can be observed by counting the success rate of 50 tasks and the incubation period for completing the tasks of the mice.
And the 7 th to 8 th days are cooperative behavior detection stages, and whether two mice have cooperative ability is detected on the basis of the completion of independent training, wherein the mode of cooperative behavior detection is divided into two modes, and the task difficulty is shallow and deep. The training phase 7 is a cooperative detection mode one, the experimental mice of the group A and the experimental mice of the group B are paired in pairs, and are placed in a new mouse cage (the middle of the new mouse cage is separated by a baffle plate with a vent hole) to adapt to the mice of the respective cooperative objects for 2 hours. The program is set to give illumination prompt at the beginning of each training, the group A and the group B mice are simultaneously put into the detection device, and if the group A mice enter and stay in the first observation area for 3 seconds or more within 10 seconds, the reward device 4 gives a sugar bean reward in the second observation area. Or when the group B mice enter and stay in the second observation area for 3 seconds or more, the reward device 4 gives a sugar bean reward to the first observation area, and the illumination prompt is closed after the reward device 4 gives the reward. After 20 seconds of interval, training is finished and is recorded as successful training; if the mice in the A group and the B group do not enter, enter the wrong observation area or enter and stay in the correct observation area for less than 3 seconds within 10 seconds, no rewards are put in, the illumination prompt is closed, and after 20 seconds of interval, training is finished and recorded as failure of training. Day 8 is partnership behavior detection mode two: in this stage, the bonus mode is slightly adjusted, the program is set to put the mice in the A group and the B group into the detection device at the same time, when one of the mice in the A group or the B group enters the correct observation area without any prompt and stays for 3 seconds or longer, the other mouse is immediately given an illumination prompt, and if the other mouse enters the correct observation area within 10 seconds and stays for 3 seconds or longer, the bonus device 4 gives a sugar bean bonus at the same time in the first observation area and the B; if the mouse does not enter the wrong viewing zone within 10 seconds or enters and stays in the right viewing zone for less than 3 seconds, both cooperating mice cannot be rewarded. By counting the success rate of 50 tasks and the incubation period for completing the tasks of the mice, the mastering degree of the mice on the test rules and the cooperative behaviors of the mice can be observed.
Claims (9)
1. The detection method of the mouse cooperative behavior is characterized by comprising a food limiting stage, a training stage and a cooperative behavior detection stage; feeding the experimental mice in a single cage in a food limiting stage, wherein the food limiting is not limited to water, recording the weight of the experimental mice every day and controlling the weight of the experimental mice to be 80-90% of a baseline;
in the training stage, experimental mice are randomly divided into a group A and a group B, and are respectively trained independently and mastered with behavior test rules, and the training stage comprises three steps: step one, adapting and mastering rules for triggering rewards by experimental mice; secondly, training the experimental mice repeatedly for a plurality of times every day by introducing specific rules to ensure that the experimental mice master the rules step by step; thirdly, strengthening the mastering of rules by the experimental mice by continuously shortening the duration of each test;
and in the cooperative behavior detection stage, after the independent training stage, taking and pairing one experimental mouse of the group A and one experimental mouse of the group B, and starting detection of two different cooperative modes after the two experimental mice are adapted to each other: one is that one mouse completes the task and the other mouse gets rewarded within a prescribed test time; after one mouse finishes the task, the other mouse finishes the task within the specified test time, and both sides obtain rewards, otherwise, both sides cannot obtain rewards; the mastering degree of the mice on the test rules and the cooperative behavior of the mice are observed by counting the success rate of the tasks of the mice and the latency of completing the tasks each time;
training phase first phase-establish rewards rules: dividing all experimental mice into A, B groups at random, and defining a sector area with the radius of 8 cm at any angle of the device as a first observation area and a sector area with the same radius at the opposite angle as a second observation area; when the group A mice enter and stay in the first observation area for more than or equal to 3 seconds, giving a sugar bean reward to the second observation area through the reward device (4), wherein a work prompt is placed in the first observation area; when the mice in the group B enter and stay in the second observation area for more than or equal to 3 seconds, giving a sugar bean reward to the first observation area through a reward device (4), training for 1 hour every day without limiting the acquisition times of the reward, wherein the whole training process is accompanied with illumination prompt, and the second observation area is provided with a working prompt different from the first observation area;
training phase second phase-set up task rules: repeating training for two consecutive days every day, wherein the experimental mice need to finish tasks and obtain rewards within a specified time, the stage is to give illumination prompts to the experimental mice at the beginning of each training, when the mice in the group A and the group B enter within 30 seconds and stay in the first observation area and the second observation area for 3 seconds or more respectively, a sugar bean rewards is given to the first observation area and the second observation area through a reward device (4), the illumination prompts are closed after the rewards are given through the reward device (4), and the training is finished and recorded as successful training once after 20 seconds; if the mice in the group A and the group B do not enter or enter within 30 seconds and continuously stay in the first observation area and the second observation area for less than 3 seconds respectively, no rewarding is put in, illumination prompt is closed, training is finished after 20 seconds, the training is recorded as failure once, and the mastering degree of the mice on the rules can be observed by counting the success rate of tasks and the incubation period for completing the tasks of all times of the mice;
training phase third phase—strengthening task rules: repeating the reinforcement training every day for two continuous days, reducing the task time after the mice master the rules, and finishing the tasks and obtaining rewards in the time of the reinforcement training experiment mice; the procedure is that the experimental mice are given illumination prompts at the beginning of each training, the mice in the A group and the B group respectively enter correspondingly within 20 seconds and 10 seconds and stay in the first observation area and the second observation area continuously for 3 seconds or more, a sugar bean reward is given to the first observation area and the second observation area through the reward device (4), the illumination prompts are closed after the reward is given through the reward device (4), and after 20 seconds of interval, the training is finished and recorded as successful training is carried out once; if the mice in the group A and the group B do not enter or enter within 20 seconds and 10 seconds respectively and continuously stay in the first observation area and the second observation area for less than 3 seconds, no rewarding is put in, illumination prompts are closed, training is finished after 20 seconds, the training is recorded as failure once, and the mastering degree of the mice on the rules can be observed by counting the success rate of tasks and the incubation period for completing the tasks of all times of the mice.
2. The method for detecting the cooperative behavior of the mouse according to claim 1, wherein the adopted device comprises an experiment box body and a control system, wherein the top of the experiment box body is provided with a camera system and an acousto-optic system, the camera system is connected with the input end of the control system, and the control signal input end of the acousto-optic system is connected with the output end of the control system; the side surface of the experiment box body is provided with a reward device (4), the side surface of the experiment box body is semitransparent, and the control signal input end of the reward device (4) is connected with the output end of the control system; the bottom surface of the box body is marked with a first observation area and a second observation area which are symmetrically distributed, the first observation area and the second observation area are arranged at the opposite angles of the bottom surface of the box body, and the output port of the rewarding device (4) extends into the experimental box body.
3. The method for detecting the cooperative behavior of the mice according to claim 2, wherein the side plate (1) of the experiment box body is made of a semitransparent acrylic plate, and the experiment box body is provided with a side door.
4. The method for detecting the cooperative behavior of a mouse according to claim 2, wherein the reward device (4) adopts an automatic feeder controlled by a TTL signal, and an output port of the automatic feeder is located in a first observation area and a second observation area, and the first observation area and the second observation area are both sector areas.
5. The method for detecting the cooperative behavior of the mice according to claim 2, wherein the experiment box comprises a top plate (2), a bottom plate (3) and an experiment box side plate (1), the experiment box side plate (1) is arranged between the top plate (2) and the bottom plate (3), and the experiment box side plate (1) is detachably connected with the bottom plate (3) and the top plate (2).
6. The method for detecting the cooperative behavior of the mice according to claim 5, wherein grooves for being filled in the side plate (1) of the experimental box body are formed in the connection parts of the top plate (2) and the bottom plate (3) and the side plate (1) of the experimental box body, and the grooves are in interference fit with the side plate (1) of the experimental box body.
7. The method for detecting cooperative behavior of mice according to claim 1, wherein all experimental mice are fed in a single cage and initial body weight is recorded in a feeding limiting stage of 3 to 0 days before the start of the experiment, a small amount of mouse food and sugar beans are fed in the morning and evening each day with unlimited water feeding, and the change of body weight of the experimental mice in the whole behavior test process is recorded.
8. The method for detecting cooperative behavior of mice according to claim 1, wherein the cooperative detection mode one, pairing group a and group B experimental mice two by two, placing the paired experimental mice in a new cage to adapt to respective cooperative object mice for 2 hours, separating the new cage by a partition plate with a vent hole, setting the stage to give illumination prompt at the beginning of each training, putting group a and group B mice into the detection device at the same time, and giving a sugar bean prize in a second observation area by a prize device (4) if the group a mice enter and stay in the first observation area for 3 seconds or more within 10 seconds, or giving a sugar bean prize in the first observation area by a prize device (4) if the group B mice enter and stay in the second observation area for 3 seconds or more, and turning off the illumination prompt after the prize device (4) is put in; after 20 seconds of interval, training is finished and is recorded as successful training; if the mice in the A group and the B group do not enter, enter the wrong observation area or enter and stay in the correct observation area for less than 3 seconds within 10 seconds, no rewards are put in, the illumination prompt is closed, and after 20 seconds of interval, training is finished and recorded as failure of training.
9. The method for detecting mouse cooperative behavior according to claim 1, wherein the cooperative behavior detection mode two: the method comprises the steps of adjusting a rewarding mode, setting a group A mouse and a group B mouse into a detection device at the same time, immediately giving a lighting prompt to one mouse when the other mouse enters a correct observation area and stays for 3 seconds or longer under the condition that no prompt exists, and giving a sugar bean rewarding to the other mouse at the same time in a first observation area and a second observation area through a rewarding device (4) if the other mouse enters the correct observation area and stays for 3 seconds or longer within 10 seconds; if the mice do not enter, enter the wrong observation area or enter and stay in the correct observation area for less than 3 seconds within 10 seconds, two cooperative mice cannot obtain rewards, and the mastery degree of the mice on the test rules and the cooperative behavior of the mice are observed by counting the task success rate of the mice and the latency of completing the tasks each time.
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| CN108094229A (en) * | 2017-12-25 | 2018-06-01 | 南京医科大学 | A kind of Behavior test platform and method for detecting animal cooperation behavior |
| WO2018155596A1 (en) * | 2017-02-24 | 2018-08-30 | 国立大学法人新潟大学 | Learning device for mice, learning system for mice, and leaning program for mice |
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| CN108094229A (en) * | 2017-12-25 | 2018-06-01 | 南京医科大学 | A kind of Behavior test platform and method for detecting animal cooperation behavior |
| CN108633771A (en) * | 2018-05-17 | 2018-10-12 | 郑州大学 | Learning training system is synchronized for animal population brain electricity |
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