CN114903038A

CN114903038A - Plant source mosquito-repellent incense and preparation method thereof

Info

Publication number: CN114903038A
Application number: CN202210383232.4A
Authority: CN
Inventors: 田厚军; 魏辉; 陈勇; 林硕; 杨风花; 陈艺欣; 郑月琴; 赵建伟
Original assignee: Institute of Plant Protection of FAAS
Current assignee: Institute of Plant Protection of FAAS
Priority date: 2022-04-12
Filing date: 2022-04-12
Publication date: 2022-08-16
Anticipated expiration: 2042-04-12
Also published as: CN114903038B

Abstract

The invention discloses a botanical mosquito-repellent incense which comprises the following raw materials in parts by weight: 3-8 parts of p-cymene, 1-3 parts of alpha-pinene, 4-8 parts of alpha-terpinene, 4-8 parts of isoascaridole, 1-3 parts of beta-ionone, 1-3 parts of D-limonene, 4-8 parts of 1- (1, 4-dimethyl-3-cyclohexene-1-yl) ethanone and 80-100 parts of starch. The invention also discloses a preparation method of the botanical mosquito-repellent incense, which comprises the following steps: (1) weighing the raw materials; (2) adding distilled water into starch for soaking, and then heating to obtain gelatinized starch; (3) uniformly mixing the raw materials except the starch to obtain plant source powder; (4) adding gelatinized starch into plant source powder, uniformly mixing to obtain a powder slurry, then stamping and forming the powder slurry, and finally drying to obtain the starch. The botanical mosquito-repellent incense is harmless to human bodies, and has the characteristics of ecological environmental protection, safety, health, simple use, convenience in carrying, rich raw materials, low cost and the like.

Description

Plant source mosquito-repellent incense and preparation method thereof

Technical Field

The invention relates to the technical field of mosquito and fly prevention and control, in particular to a botanical mosquito-repellent incense and a preparation method thereof.

Background

Mosquito-repellent incense as a daily necessity in summer has mosquito-repellent effect, and the mosquito can be driven away or fumigated by smoke emitted by ignited drugs, so that the use of the mosquito-repellent incense has a long history. The traditional mosquito-repellent incense in China mainly comprises combustion substrates such as carbon powder, coal powder, cellulose and the like and chemical insecticides (such as pyrethroid pesticides), smoke generated by combustion during use pollutes indoor air, mosquitoes can generate drug resistance to the mosquito-repellent incense after long-time use, the effect is seriously reduced, and more importantly, the traditional mosquito-repellent incense also has certain toxicity to human bodies.

Recently introduced electrically heated mosquito coils also contain an insecticide component. Although various electronic mosquito-repellent incense comes out endlessly, products for repelling mosquitoes by utilizing sound waves have not been reported to have definite effects. The mosquitoes have certain sensing capability on the ultrasonic wave, but because of the complexity of mosquito communication, no experiment proves which sound wave is effective for repelling the mosquitoes at all, and the effect is far less than that of the traditional mosquito repellent by simply relying on the sound wave.

The plant source substance is derived from plant secondary metabolites, so that the synergistic effect of the insect pheromone can be enhanced, and the synergistic effect is realized among different plant source substances. Some plant-derived substances can remarkably enhance the action efficiency of insect information substances or functional substances to further regulate and control the behavior of insects, and the synergistic action on the insect information substances or the functional substances is mediated by octopamine and receptors thereof in the insects. When certain plant-derived substances bind to the octopamine receptor, lowering the reaction threshold of the reporter or functional substance-receiving neurons to the substance and increasing the sensitivity of the substance-receiving neurons are also important mechanisms for the potentiation of plant-derived substances. In order to further produce the mosquito-repellent and mosquito-repellent incense which is ecological and environment-friendly and is friendly to human health, scientific researchers constantly change and optimize the components of the mosquito-repellent incense, plant source materials are added, and the mosquito-repellent effect of the mosquito-repellent incense is verified through insect ethology experiments. However, different mosquito incense manufacturing processes and the composition and the proportion of each material restrict the manufacturing cost and the mosquito repelling effect of the mosquito incense.

Therefore, the key to the function of the mosquito coil is to screen the plant source substances and adjust the proportion of the plant source substances.

Disclosure of Invention

In view of the above, the invention aims to provide a botanical mosquito-repellent incense and a preparation method thereof, which can achieve a good mosquito-repellent effect by selecting trace amounts of botanical substances and solve the problems of excessive pesticide components, easy generation of resistance and complex preparation in the existing mosquito-repellent incense. After the botanical mosquito-repellent incense is used for 2 hours, the indoor mosquito density is averagely reduced by over 97.5 percent, and good mosquito-repellent effect is achieved.

In order to achieve the purpose, the invention adopts the following technical scheme:

a botanical mosquito-repellent incense comprises the following raw materials in parts by weight: 3-8 parts of p-cymene, 1-3 parts of alpha-pinene, 4-8 parts of alpha-terpinene, 4-8 parts of isoascaridole, 1-3 parts of beta-ionone, 1-3 parts of D-limonene, 4-8 parts of 1- (1, 4-dimethyl-3-cyclohexene-1-yl) ethanone and 80-100 parts of starch.

Preferably: 8 parts of para-cymene, 3 parts of alpha-pinene, 8 parts of alpha-terpinene, 8 parts of isoascaridole, 3 parts of beta-ionone, 3 parts of D-limonene, 8 parts of 1- (1, 4-dimethyl-3-cyclohexene-1-yl) ethanone and 100 parts of starch.

Preferably: 7 parts of para-cymene, 2 parts of alpha-pinene, 7 parts of alpha-terpinene, 7 parts of isoascaridole, 2 parts of beta-ionone, 2 parts of D-limonene, 7 parts of 1- (1, 4-dimethyl-3-cyclohexene-1-yl) ethanone and 90 parts of starch.

Preferably: 6 parts of para-cymene, 3 parts of alpha-pinene, 6 parts of alpha-terpinene, 6 parts of isoascaridole, 3 parts of beta-ionone, 2 parts of D-limonene, 6 parts of 1- (1, 4-dimethyl-3-cyclohexene-1-yl) ethanone and 80 parts of starch.

In the invention, the functions and effects of the raw materials are as follows:

para-cymene is a mosquito repellent and is derived from plant extracts.

The alpha-pinene is a mosquito repellent and is derived from plant extracts.

The alpha-terpinene is a mosquito repellent and is derived from plant extracts.

Ascaridol is a mosquito repellent and is derived from plant extracts.

The beta-ionone is a mosquito repellent, is derived from plant essential oil, and has fragrance components.

The D-limonene is a mosquito repellent, is derived from plant essential oil, and has a fragrance component.

The 1- (1, 4-dimethyl-3-cyclohexene-1-yl) ethanone can be used as a perfume component and has a certain repellent effect on mosquitoes.

The addition of the starch can prolong the burning time of the mosquito incense, permanently play the repelling effect and have the functions of a combustion improver and an adhesive.

A preparation method of plant source mosquito-repellent incense specifically comprises the following steps:

(1) weighing the raw materials in parts by weight of the plant source mosquito-repellent incense;

(2) firstly, adding distilled water into starch for soaking, and then heating to obtain gelatinized starch for later use;

(3) uniformly mixing p-cymene, alpha-pinene, alpha-terpinene, isoascaridole, beta-ionone, D-limonene and 1- (1, 4-dimethyl-3-cyclohexene-1-yl) ethanone to obtain plant source powder;

(4) adding gelatinized starch into plant source powder, uniformly mixing to obtain a powder slurry, then stamping and forming the powder slurry, and finally drying to obtain the plant source mosquito-repellent incense.

Further, in the step (2), the adding amount of the distilled water is 2-3 times of the mass of the starch; soaking for 25 min; the heating time is 60-80 ℃.

The further technical scheme has the beneficial effects that the gelatinized starch is prepared by soaking and heating the starch, so that the dissolving speed is high and the adhesion is strong.

Further, in the step (4), the stamping device is a incense maker. Further, the powder slurry is put into a incense making machine to be punched into strips or sheets.

According to the technical scheme, compared with the prior art, the invention has the following beneficial effects:

1. the botanical mosquito-repellent incense is harmless to human bodies, and has the characteristics of ecological environmental protection, safety, health, simple use, convenience in carrying, rich raw materials, low cost and the like.

2. The plant source components in the plant source mosquito-repellent incense have the synergistic interaction effect, so that mosquitoes are not easy to generate resistance, the safety performance is high, the ignited mosquito-repellent incense contains perfume smell and also has the effect of repelling and killing insects, and meanwhile, the mosquito-repellent incense also has the obvious poisoning effect on epidemic germs and has the advantages of health care and sanitation.

3. The plant source substance is compounded, pesticide ingredients contained in the traditional mosquito-repellent incense are comprehensively replaced, all the components are uniformly and slowly released in starch, no smoke or dust is generated after ignition, no pollution is caused to the environment, and the safety performance is high.

4. The behavior of insects can be further regulated by enhancing synergistic effect between different plant-derived substances, and when one plant-derived substance is combined with octopamine receptor, the reaction threshold of another plant-derived substance-receiving neuron on the substance is reduced, the sensitivity of the substance-receiving neuron is enhanced, and the action effect of the substance is improved. The components in the botanical mosquito-repellent incense are all derived from substances extracted from plants, are various in types, have a certain synergistic interaction effect, and enable mosquitoes not to easily generate drug resistance.

Drawings

FIG. 1 shows the mosquito repellent rate in response to the directional behavior of plant-derived mosquito coil mixtures and single components (Note: marked by differences).

Detailed Description

The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1

The botanical mosquito-repellent incense comprises the following raw materials by weight: 8g of para-cymene, 3g of alpha-pinene, 8g of alpha-terpinene, 8g of isoascaridole, 3g of beta-ionone, 3g of D-limonene, 8g of 1- (1, 4-dimethyl-3-cyclohexene-1-yl) ethanone and 100g of starch;

the preparation method of the plant source mosquito-repellent incense specifically comprises the following steps:

(1) weighing the raw materials according to the weight parts of the plant source mosquito-repellent incense;

(2) adding distilled water with the mass of 3 times into starch, soaking for 25min, and heating at 70 ℃ to obtain gelatinized starch for later use;

(4) adding gelatinized starch into plant source powder, uniformly mixing to obtain powder slurry, then putting the powder slurry into a fragrance making machine, punching the powder slurry into strips, and finally drying to obtain the plant source mosquito-repellent incense.

Example 2

The botanical mosquito-repellent incense comprises the following raw materials by weight: 7g of p-cymene, 2g of alpha-pinene, 7g of alpha-terpinene, 7g of isoascaridole, 2g of beta-ionone, 2g of D-limonene, 7g of 1- (1, 4-dimethyl-3-cyclohexene-1-yl) ethanone and 90g of starch;

(2) adding distilled water with the mass of 3 times into starch, soaking for 25min, and heating at 80 ℃ to obtain gelatinized starch for later use;

Example 3

The botanical mosquito-repellent incense comprises the following raw materials by weight: 6g of p-cymene, 3g of alpha-pinene, 6g of alpha-terpinene, 6g of isoascaridole, 3g of beta-ionone, 2g of D-limonene, 6g of 1- (1, 4-dimethyl-3-cyclohexene-1-yl) ethanone and 80g of starch;

(2) adding 2 times of distilled water into starch, soaking for 25min, and heating at 70 deg.C to obtain gelatinized starch;

(4) adding gelatinized starch into plant source powder, uniformly mixing to obtain powder slurry, then putting the powder slurry into a fragrance making machine, punching into sheets, and finally drying to obtain the plant source mosquito-repellent incense.

Comparative example 1

The only difference from example 1 is that the starting material does not contain p-cymene.

Comparative example 2

The only difference from example 1 is that the feed does not contain alpha-terpinene.

Comparative example 3

The only difference from example 1 is that the feed does not contain isoascaridol.

Comparative example 4

The only difference from example 1 is that the starting material does not contain beta-ionone and D-limonene.

Comparative example 5

The only difference from example 1 is that the feed does not contain alpha-terpinene and D-limonene.

Comparative example 6

The only difference from example 1 is that the starting material contains only p-cymene and starch.

Comparative example 7

The only difference from example 1 is that the starting material contains only alpha-pinene and starch.

Comparative example 8

The only difference from example 1 is that the feedstock contains only alpha-terpinene and starch.

Comparative example 9

The only difference from example 1 is that the feed contained exclusively isoascaridol and starch.

Comparative example 10

The only difference from example 1 is that the starting material contains only beta-ionone and starch.

Comparative example 11

The only difference from example 1 is that the starting material contains only D-limonene and starch.

Comparative example 12

The only difference from example 1 is that the starting material contains only 1- (1, 4-dimethyl-3-cyclohexen-1-yl) ethanone and starch.

Performance testing

1. Determination of Directional behavior response of mosquitoes to plant-derived mosquito incense mixtures and Single Components

The directional behavior reaction of the mosquito to the plant source mosquito incense mixture and the single component is measured by a Y-shaped olfactometer (improved according to the basic principle of plant protection research institute, grant publication No. CN201726758U and utility model name 'Y-shaped behavior measuring instrument' of the agricultural academy of sciences of Fujian province). The main arm of the Y-shaped pipe is 20cm, the lengths of the two arms are respectively 10cm, and the included angle between the two arms is 90 ℃. The two arm tubes are sequentially connected with a distilled water bottle and an active carbon bottle by a silica gel tube, and finally the active carbon bottle is opened in the atmosphere, and the insect release bottle is connected with an air extractor by gauze. The rubber head carrier was placed in the trap inlet glass tube.

According to preliminary experiments, the plant source components have good repellent effect on Culex pipiens pallens (Culex pipiens) at the concentration of 0.01-0.5 ug/uL. Thus:

(1) paraffin oil is adopted to respectively prepare p-cymene, alpha-pinene, alpha-terpinene, isoascaridole, beta-ionone, D-limonene and 1- (1, 4-dimethyl-3-cyclohexene-1-yl) ethanone into single-component solutions with the concentration of 0.1ug/uL for behavior test.

(2) Para-cymene, alpha-pinene, alpha-terpinene, isoascaridole, beta-ionone, D-limonene and 1- (1, 4-dimethyl-3-cyclohexen-1-yl) ethanone were formulated in parts by weight of 8:3:8:8:3:3:8 (i.e., the example 1 component without starch) with paraffin oil to a mixture solution with a total concentration of 0.1ug/uL for behavioral testing.

Respectively weighing 5mL of each single-component solution and the mixture solution, dripping the single-component solution and the mixture solution into a flavor source bottle on one side, taking paraffin oil dripping 5mL as a control on the other side, controlling the flow rate of air flow of air extraction to be 1L/min, and respectively filtering the air flow entering the two tubes by active carbon and humidifying the air flow by distilled water. Placing 30 culex pipiens pallens into the insect release bottle, repeating for 3 times, dipping the inner wall and the outer wall of an absolute ethyl alcohol Y-shaped tube by using a cotton ball after each measurement, drying, and then replacing two arms of the Y-shaped tube with a taste source bottle and a contrast to eliminate the possible influence of geometric errors of the two arms on the behavior of culex pallens. And (3) placing the test insects into an insect release bottle of a Y-shaped pipe to start the test, counting the test result after 10min, and judging that all culex pipiens pallens staying on the main arm of the Y-shaped pipe are non-reactive. The results are shown in FIG. 1.

As can be seen from fig. 1, the avoidance rates of culex pipiens pallens on the plant-derived mosquito-repellent incense mixture and the individual components (p-cymene, α -pinene, α -terpinene, isoascaridole, β -ionone, D-limonene and 1- (1, 4-dimethyl-3-cyclohexen-1-yl) ethanone) are 100%, 84.44%, 73.33%, 80.00%, 81.11%, 71.11%, 74.44% and 56.67%, respectively, which are significantly higher than those of the control (paraffin oil) of each group.

2. Mosquito repellent effect of plant source mosquito repellent incense

The test is carried out according to the method for measuring the indoor drug effect of the mosquito-repellent incense and the evaluation standard specified in GB/T13917.4-2009. The specific method comprises the following steps:

3000 culex pipiens pallens collected and propagated outdoors in 6-7 months in summer are averagely divided into 15 groups of 200 heads each, and the 3 rd to 5 th day after emergence do not take blood. At 20m ² The test is carried out in a ventilated and air-permeable closed room with a screen window, the temperature is 20-30 ℃ at room temperature, and the relative humidity is 55-80%; the plant-derived mosquito incense prepared in examples 1 to 3 and comparative examples 1 to 12, respectively, was ignited at both corners and the center of the room in diagonal lines, and then prepared culex pipiens was housed in the room, and the knockdown rate of culex pipiens was counted after 1h and 2h, respectively, and calculated.

The results are shown in Table 1.

TABLE 1 mosquito repellent Effect of examples 1-3 and comparative examples 1-12 botanical mosquito incense

As can be seen from Table 1, the plant-derived mosquito incense obtained in examples 1 to 3 of the present invention has a knockdown rate of at least 95% for culex pipiens in 1 hour and a knockdown rate of at least 97.5% for 2 hours.

The comparative examples 1 to 5 show the mosquito repellent effect when 1 to 2 components are absent in the botanical mosquito repellent incense, the knockdown rate of 1h of culex pipiens pallens can reach 80 percent at the lowest, and the knockdown rate of 2h can reach 82.5 percent at the lowest, compared with the examples 1 to 3, the mosquito repellent effect of 1h can be reduced by 18 percent at the highest, and the mosquito repellent effect of 2h can be reduced by 17.5 percent at the highest.

Comparative examples 4 to 12 are the mosquito repellent effects of the plant-derived mosquito coil containing only a single component, and the knockdown rate for culex pipiens with light color for 1 hour is only 30.0% at the lowest, and is only 52.5% at the highest, and the knockdown rate for 2 hours is 31.5% at the lowest, and is 55% at the highest, compared with examples 1 to 3 of the present invention, the mosquito repellent effect is reduced by nearly 70% at the highest, and the single component mosquito repellent effect is far lower than that of the plant-derived mosquito coil of the present invention.

The tests show that the plant source mosquito-repellent incense has very good mosquito-repellent and mosquito-repellent effects. The comparative examples 1 to 5 lack 1 to 2 components respectively, the comparative examples 4 to 12 only contain a single component, the mosquito repellent effect shows that the components of the invention have certain synergistic effect, each component has certain effect, the volatile odor acts on the taxis of insects mainly by influencing the antennal of the insects and sensors of the whole body, namely the combination of the odor molecules and the odor receptors on the dendrites of olfactory neurons, the combination capability of different odor molecules and the neuron odor receptors of the olfactory sensors and the combination quantity in unit time are different, the types of the neurons and the odor receptors on the dendrites are many, the types of the odor molecules which can be combined with the neurons are also many, and therefore, the combination type and the quantity of the odor molecule compound with the same effect and the olfactory neuron receptors are higher than those of the single component. Moreover, the components of the invention are from evasion plants in nature, and the compound effect is the best after extraction identification and single-component and compound behavior determination.

The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. The botanical mosquito-repellent incense is characterized by comprising the following raw materials in parts by weight: 3-8 parts of p-cymene, 1-3 parts of alpha-pinene, 4-8 parts of alpha-terpinene, 4-8 parts of isoascaridole, 1-3 parts of beta-ionone, 1-3 parts of D-limonene, 4-8 parts of 1- (1, 4-dimethyl-3-cyclohexene-1-yl) ethanone and 80-100 parts of starch.

2. The botanical mosquito incense coil as claimed in claim 1, which is characterized by comprising the following raw materials in parts by weight: 8 parts of para-cymene, 3 parts of alpha-pinene, 8 parts of alpha-terpinene, 8 parts of isoascaridole, 3 parts of beta-ionone, 3 parts of D-limonene, 8 parts of 1- (1, 4-dimethyl-3-cyclohexene-1-yl) ethanone and 100 parts of starch.

3. The botanical mosquito incense coil as claimed in claim 1, which is characterized by comprising the following raw materials in parts by weight: 7 parts of para-cymene, 2 parts of alpha-pinene, 7 parts of alpha-terpinene, 7 parts of isoascaridole, 2 parts of beta-ionone, 2 parts of D-limonene, 7 parts of 1- (1, 4-dimethyl-3-cyclohexene-1-yl) ethanone and 90 parts of starch.

4. The botanical mosquito-repellent incense according to claim 1, comprising the following raw materials in parts by weight: 6 parts of para-cymene, 3 parts of alpha-pinene, 6 parts of alpha-terpinene, 6 parts of isoascaridole, 3 parts of beta-ionone, 2 parts of D-limonene, 6 parts of 1- (1, 4-dimethyl-3-cyclohexene-1-yl) ethanone and 80 parts of starch.

5. A preparation method of plant source mosquito-repellent incense is characterized by comprising the following steps:

(1) weighing the raw materials according to the parts by weight of the plant source mosquito-repellent incense of any one of claims 1 to 4;

(2) adding distilled water into starch for soaking, and then heating to obtain gelatinized starch for later use;

(4) adding gelatinized starch into plant source powder, uniformly mixing to form slurry, then stamping the slurry into shape, and finally drying to obtain the plant source mosquito-repellent incense.

6. The method for preparing the plant source mosquito-repellent incense according to claim 5, wherein in the step (2), the addition amount of the distilled water is 2-3 times of the mass of the starch.

7. The method for preparing the plant-derived mosquito incense according to claim 5, wherein in the step (2), the soaking time is 25 min.

8. The method for preparing mosquito incense made of plant source according to claim 5, wherein in the step (2), the heating time is 60-80 ℃.

9. The method for preparing the botanical mosquito-repellent incense according to claim 5, wherein in the step (4), the stamping device is a incense maker.

10. The method for preparing a plant source mosquito-repellent incense according to claim 5 or 9, wherein in the step (4), the slurry is put into a incense making machine and is punched into strips or sheets.