IIII type recombinant collagen hydrogel preparation equipment and application method thereof
Technical Field
The invention relates to the technical field of collagen preparation equipment, in particular to III type recombinant collagen hydrogel preparation equipment and a use method thereof.
Background
Collagen is a biopolymer, the main component in animal connective tissue, and is also the functional protein with the greatest content and the greatest distribution in mammals, accounting for 25% -30% of the total protein, and some organisms even up to more than 80%, animal tissues of livestock and poultry are the main way for people to obtain natural collagen and collagen peptide, but the use of the collagen and products thereof for land mammals is limited by related livestock diseases and certain religious beliefs, and the development of the collagen and products thereof is gradually turned to marine organisms. The European Food Safety Agency (EFSA) has confirmed that even the collagen derived from animal bones is not likely to infect mad cow disease and other related diseases, and the abundant collagen contained in skin, bones and scales of aquatic animals, especially the processing wastes of aquatic animals, has the advantages not available in many livestock collagens due to the difference of amino acid composition, crosslinking degree and the like, and in addition, the collagen derived from marine animals is obviously superior to the collagen of terrestrial animals in some aspects, such as low antigenicity, low allergy and the like. Therefore, the marine collagen may gradually replace the collagen of terrestrial animals, and the collagen is of various types, such as type I, type II, type III, type V and type XI.
In the process of preparing collagen, a centrifuge is required to perform centrifugal separation of the collagen liquid.
However, in the use process of the centrifugal machine, the centrifugal tube may be broken, so that collagen liquid in the centrifugal tube is leaked, and the tube grooves on the existing rotor are long in depth so as to adapt to the centrifugal tube with different lengths, so that once the centrifugal tube is broken, a large amount of collagen liquid can be thrown to the bottom of the tube grooves under the action of centrifugation, the distance between the collagen liquid and the circle center of the rotor is changed, the rotor is in an unbalanced state, the rotor is caused to shake, and once a worker does not timely turn off a power supply, the service life of the rotor is greatly shortened, and serious problems such as rotor breakage and flying out are caused, so that potential safety hazards are caused.
Disclosure of Invention
1. Technical problem to be solved
Aiming at the problems in the prior art, the invention aims to provide the III type recombinant collagen hydrogel preparation equipment and the use method thereof, when a centrifuge tube breaks, the leaked collagen liquid can push the magnetic scraping disc by utilizing the centrifugal force generated when the rotor rotates at a high speed, the magnetic scraping disc is promoted to stir a plurality of sound stirring sheets to generate reminding sound, so that a worker is informed of the leakage of the current centrifuge tube, the worker is promoted to timely close the total power supply of the centrifuge, the shaking time of the rotor is reduced, the damage to the rotor is reduced, meanwhile, the distance between the collagen liquid and the center of the rotor is changed by matching with the arrangement of the electromagnet and the magnetic scraping disc, so that the collagen liquid can be discharged into an annular bag with the distance between the center of the rotor and the center of the centrifuge tube, the unbalanced state generated when the rotor rotates at a high speed can be relieved, and the damage caused by shaking of the rotor is reduced to a certain extent before the worker closes the power supply.
2. Technical proposal
In order to solve the problems, the invention adopts the following technical scheme.
The utility model provides a III type recombinant collagen hydrogel preparation equipment and application method thereof, includes centrifuge and installs the inside rotor at the centrifuge, the excavation has a plurality of evenly distributed's tube nest on the rotor, and a plurality of tube nest are the slope setting, the inside grafting of tube nest has the centrifuging tube, a plurality of the tube nest is inside all to be provided with the funnel that is located the centrifuging tube downside, funnel lower extreme fixedly connected with one-way flow tube, fixedly connected with storage bag between one-way flow tube and the tube nest downside inner wall, sliding connection has magnetism to scrape the dish on the storage bag, tube nest inside is provided with annular bag, and the centrifuging tube grafting is on annular bag, peg graft on the annular bag and have a plurality of infusion tubules that are linked together, and a plurality of infusion tubules run through the storage bag setting, the excavation has a plurality of row mouths that are located the storage bag inside on the infusion tubule, rotor internally mounted has the electro-magnet that is located the tube nest below, install shift knob on the tube downside inner wall, shift knob and electro-magnet electric connection.
Further, a plurality of sound pulling sheets positioned on the left side and the right side of the storage bag are fixedly connected in the pipe groove, the reminding effect can be achieved, the centrifuge tube is informed of breakage of a worker, and the worker is prompted to close the centrifuge main power supply in time.
Further, a plurality of the same spacing stick of fixedly connected with between infusion tubule and the tube duct lower side wall, magnetism scrape dish center department and open to have with spacing stick assorted scrape the hole, can play spacing effect when magnetism scrapes the dish and remove, be convenient for extrude the storage bag simultaneously to a certain extent.
Further, funnel internally mounted has the filter screen, can filter the broken sediment that produces when partly centrifuging tube breaks, avoids causing the damage to storage bag and unidirectional flow pipe.
Further, centrifuging tube lower extreme fixedly connected with seals the roof, it has a plurality of and infusion tubule assorted sockets to dig on the sealed roof to can be according to the centrifuging tube of different length, after the centrifuging tube breaks, the unbalanced state of rotor obtains the release of certain degree.
Further, the fixed bars are fixedly connected between the left side wall and the right side wall of the magnetic scraping plate and the inner wall of the pipe groove, the fixed bars are made of brittle materials, and the magnetic scraping plate cannot move due to centrifugal force generated by high-speed rotation of the rotor through the fixation of the fixed bars.
Furthermore, the upper ends of the transfusion tubules are close to the inner wall of the upper side of the annular bag, so that collagen liquid can be discharged from the upper part of the annular bag, and the transfusion tubules are suitable for centrifuge tubes with different lengths.
Furthermore, the annular bag is made of elastic materials, so that the storage capacity of the annular bag can be improved.
The application method of the III type recombinant collagen hydrogel preparation equipment comprises the following steps:
s1: pouring the unseparated collagen liquid into a plurality of centrifuge tubes, and symmetrically inserting the plurality of centrifuge tubes into tube slots respectively;
s2: starting a centrifugal machine to separate out collagen from the collagen liquid under the centrifugal action;
s3: after the staff hears the sound of the sound-poking sheet, the centrifuge tube is broken in the process, and the centrifuge main power supply is turned off immediately;
s4: and after the power supply is turned off, waiting for a period of time until the rotor stops rotating, opening the centrifugal machine, and cleaning a tube groove on the centrifugal tube inserted with the rupture.
3. Advantageous effects
Compared with the prior art, the invention has the advantages that:
(1) This scheme can take place when breaking at the centrifuging tube, utilize the centrifugal force that produces when rotor high-speed rotation, make the collagen liquid that leaks promote magnetism and scrape the dish, make magnetism scrape the dish and stir a plurality of plectrum, take place the sound of warning, thereby inform the staff, current centrifuging tube takes place to leak, make the staff in time close centrifuge's total power, reduce the time of rocking of rotor, thereby reduce the damage to the rotor, simultaneously, cooperation electro-magnet and magnetism scrape the setting of dish, can change the distance of collagen liquid and rotor centre of a circle, make it can be discharged to the centrifuging tube in the annular bag that rotor centre of a circle is close, and then can alleviate the unbalance state that takes place when rotor high-speed rotation, before the staff closes the power, the damage that produces because of rocking of the rotor has been reduced to a certain extent.
(2) The inside fixedly connected with of tube socket is located the plectrum of storage bag left and right sides, can play the effect of warning, informs the staff centrifuging tube and takes place to break, makes the staff in time close centrifuge total power.
(3) The same spacing stick of fixedly connected with between a plurality of infusion tubules and the tube chute lower lateral wall, magnetism scrape dish center department and open to have with spacing stick assorted scrape the hole, can play spacing effect when magnetism scrapes the dish and remove, be convenient for extrude the storage bag simultaneously to a certain extent.
(4) The filter screen is arranged in the funnel, so that the broken slag generated when a part of the centrifuge tube breaks can be filtered, and damage to the storage bag and the unidirectional flow tube is avoided.
(5) The centrifuge tube lower extreme fixedly connected with seals the roof, has a plurality of and infusion tubule assorted sockets on the sealed roof to can be according to the centrifuging tube of different length, after the centrifuging tube breaks, the unbalance state of rotor obtains the release of certain degree.
(6) The fixed bars are fixedly connected between the left side wall and the right side wall of the magnetic scraping plate and the inner wall of the pipe groove, the fixed bars are made of brittle materials, and the magnetic scraping plate cannot be moved due to centrifugal force generated by high-speed rotation of the rotor through the fixation of the fixed bars.
(7) The upper ends of the transfusion tubules are close to the inner wall of the upper side of the annular bag, so that collagen liquid can be discharged from the upper part of the annular bag, and the collagen liquid can be suitable for centrifuge tubes with different lengths.
(8) The annular bag is made of elastic materials, so that the storage capacity of the annular bag can be improved.
Drawings
FIG. 1 is a front cross-sectional view of the present invention;
FIG. 2 is a three-dimensional perspective view of the rotor of the present invention;
FIG. 3 is a schematic view of the structure of the inside of the pipe groove of the present invention;
FIG. 4 is a schematic view of the structure of the inside of the annular bladder of the present invention;
FIG. 5 is a schematic view of the funnel portion of the present invention;
FIG. 6 is a front cross-sectional view of a portion of a storage bladder according to the present invention;
FIG. 7 is a top cross-sectional view of a seal top plate portion of the present invention;
FIG. 8 is an enlarged view of FIG. 7 at A;
FIG. 9 is a top cross-sectional view of a magnetic wiper portion of the present invention;
fig. 10 is a diagram of the present invention.
The reference numerals in the figures illustrate:
1 a centrifugal machine, 2 a rotor, 3 a tube groove, 4 a centrifugal tube, 41 a sealing top plate, 5 a funnel, 6 a unidirectional flow tube, 7 a storage bag, 8 a magnetic scraping disc, 81 a scraping hole, 82 a fixing rod, 83 a sound-poking sheet, 9 an annular bag, 91 a transfusion thin tube, 92 a limiting rod, 10 an electromagnet and 101 a switch button.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention; it is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments, and that all other embodiments obtained by persons of ordinary skill in the art without making creative efforts based on the embodiments in the present invention are within the protection scope of the present invention.
In the description of the present invention, it should be noted that the positional or positional relationship indicated by the terms such as "upper", "lower", "inner", "outer", "top/bottom", etc. are based on the positional or positional relationship shown in the drawings, are merely for convenience of describing the present invention and simplifying the description, and do not indicate or imply that the apparatus or elements referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "configured to," "engaged with," "connected to," and the like are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.
Example 1:
referring to fig. 1-6, a preparation device of type iii recombinant collagen hydrogel and a method for using the same, comprising a centrifuge 1 and a rotor 2 installed inside the centrifuge 1, wherein a plurality of evenly distributed tube slots 3 are cut on the rotor 2, the tube slots 3 are obliquely arranged, a centrifuge tube 4 is inserted in the tube slots 3, a funnel 5 positioned at the lower side of the centrifuge tube 4 is arranged in the tube slots 3, the lower end of the funnel 5 is fixedly connected with a unidirectional flow tube 6, a storage bag 7 is fixedly connected between the unidirectional flow tube 6 and the inner wall at the lower side of the tube slots 3, a magnetic scraping disc 8 is slidingly connected on the storage bag 7, an annular bag 9 is arranged in the tube slots 3, the centrifuge tube 4 is inserted on the annular bag 9, a plurality of communicated transfusion tubules 91 are inserted on the annular bag 9, the transfusion tubules 91 penetrate through the storage bag 7, a plurality of discharge ports positioned in the storage bag 7 are cut on the transfusion tubules 91, the rotor 2 is internally provided with an electromagnet 10 positioned below the tube groove 3, the inner wall of the lower side of the tube groove 3 is provided with a switch button 101, the switch button 101 is electrically connected with the electromagnet 10, after the centrifuge 1 is started, collagen liquid is separated under the action of centrifugation, at the moment, the left and right side walls of the magnetic scraping disc 8 and the inner wall of the tube groove 3 are fixedly connected with fixing rods 82, the fixing rods 82 are made of brittle materials, the centrifugal force generated by the high-speed rotation of the rotor 2 is generated by the fixing of the fixing rods 82, the movement of the magnetic scraping disc 8 is not caused, when the centrifugal tube 4 breaks in the process, the collagen liquid in the centrifugal tube 4 is thrown into the funnel 5 under the action of the centrifugation and enters the storage bag 7 through the unidirectional flow tube 6, so that the storage bag 7 above the magnetic scraping disc 8 expands under the action of centrifugation, the thrust to magnetism scraping plate 8 has been increaseed, make fixed stick 82 take place to fracture, make magnetism scraping plate 8 take place to remove, the inside fixedly connected with of tube groove 3 is a plurality of plectrum 83 that are located the left and right sides of storage bag 7, at the in-process that magnetism scraping plate 8 removed, can constantly stir a plurality of plectrums 83, thereby take place the sound of warning, inform staff centrifuging tube 4 emergence leakage, make the staff in time close centrifuge 1's total power, reduce rotor 2's the length of rocking, thereby reduce the damage to rotor 2, follow-up removal of magnetism scraping plate 8 can press shift knob 101, thereby switch on electro-magnet 10 is opened, promote magnetism scraping plate 8 through the electro-magnet 10 and extrude the storage bag 7 of inflation, make the inside collagen liquid of storage bag 7 extrude to annular bag 9 inside through a plurality of spacing sticks 92, as shown in fig. 10.
Referring to fig. 7-9, the same limiting rod 92 is fixedly connected between the plurality of infusion tubules 91 and the lower side wall of the tube slot 3, a scraping hole 81 matched with the limiting rod 92 is cut at the center of the magnetic scraping plate 8, so that the limiting effect can be achieved when the magnetic scraping plate 8 moves, meanwhile, the storage bag 7 is convenient to extrude to a certain extent, the lower end of the centrifuge tube 4 is fixedly connected with a sealing top plate 41, a plurality of sockets matched with the infusion tubules 91 are cut on the sealing top plate 41, the sealing top plate 41 is utilized to extrude the annular bag 9, and accordingly collagen liquid can be discharged into the annular bag 9 with the center distance between the centrifuge tube 4 and the rotor 2 according to centrifuge tubes 4 with different lengths, and unbalance conditions occurring when the rotor 2 rotates at a high speed can be relieved to a certain extent, damage caused by shaking of the rotor 2 is further reduced.
Referring to fig. 5, a filter screen is installed in the funnel 5, which can play a role in filtering, and avoid damage to the storage bag 7 and the unidirectional flow tube 6 due to glass fragments generated when the centrifuge tube 4 is broken.
Referring to fig. 4, the upper ends of the plurality of infusion tubules 91 are disposed near the inner wall of the upper side of the annular sac 9, so that collagen liquid can be discharged from the upper side of the annular sac 9, thereby adapting to centrifuge tubes 4 with different lengths, the annular sac 9 is made of elastic materials, and the storage capacity of the annular sac 9 can be improved.
The application method of the III type recombinant collagen hydrogel preparation equipment comprises the following steps:
s1: pouring the unseparated collagen liquid into a plurality of centrifuge tubes 4, and symmetrically inserting the plurality of centrifuge tubes 4 into the tube slots 3 respectively;
s2: starting a centrifugal machine 1, and separating out collagen from the collagen liquid under the centrifugal action;
s3: after hearing the sound of the sound pulling sheet 83, the centrifuge tube 4 is broken in the process, and the centrifuge main power supply should be immediately turned off;
s4: after the power is turned off, a period of time is waited until the rotor 2 stops rotating, the centrifuge 1 is turned on, and the tube groove 3 on the broken centrifuge tube 4 is cleaned.
The above description is only of the preferred embodiments of the present invention; the scope of the invention is not limited in this respect. Any person skilled in the art, within the technical scope of the present disclosure, may apply to the present invention, and the technical solution and the improvement thereof are all covered by the protection scope of the present invention.