CN114874946A - Rahnella aquatilis and application thereof in sewage denitrification - Google Patents

Rahnella aquatilis and application thereof in sewage denitrification Download PDF

Info

Publication number
CN114874946A
CN114874946A CN202210599246.XA CN202210599246A CN114874946A CN 114874946 A CN114874946 A CN 114874946A CN 202210599246 A CN202210599246 A CN 202210599246A CN 114874946 A CN114874946 A CN 114874946A
Authority
CN
China
Prior art keywords
strain
sewage
nitrogen
denitrification
low
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202210599246.XA
Other languages
Chinese (zh)
Inventor
赵宇明
郑巍
刘志敏
张露
都晨
曹月
李钥玮
刘丹
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shenyang Fengjing Landscape Co ltd
Original Assignee
Shenyang Fengjing Landscape Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shenyang Fengjing Landscape Co ltd filed Critical Shenyang Fengjing Landscape Co ltd
Priority to CN202210599246.XA priority Critical patent/CN114874946A/en
Publication of CN114874946A publication Critical patent/CN114874946A/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/16—Nitrogen compounds, e.g. ammonia
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/16—Nitrogen compounds, e.g. ammonia
    • C02F2101/163—Nitrates
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/16—Nitrogen compounds, e.g. ammonia
    • C02F2101/166—Nitrites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00—Technologies for wastewater treatment
    • Y02W10/10—Biological treatment of water, waste water, or sewage

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Hydrology & Water Resources (AREA)
  • Environmental & Geological Engineering (AREA)
  • Water Supply & Treatment (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)

Abstract

The invention belongs to the technical field of biological sewage treatment and environmental microbiology, and particularly relates to Rahnella aquatilis WS33 and application thereof in removing inorganic nitrogen pollution in sewage under a medium-low temperature condition. The strain WS33 is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC No. 22997. The strain WS33 has good low-temperature resistance activity, and can be applied to the enhanced treatment process of denitrification of various sewage such as domestic sewage in cold regions or in low-temperature season periods. The strain WS33 is used for improving the sewage denitrification effect under the low-temperature condition, and has the advantages of wide application temperature range, low cost and environmental friendliness.

Description

Rahnella aquatilis and application thereof in sewage denitrification
Technical Field
The invention belongs to the technical field of environmental microbiology and sewage biological treatment, and particularly relates to an application of Rahnella aquatica WS33 in sewage denitrification.
Background
With the development of human social industry and agriculture and the promotion of urbanization, the pollution of inorganic nitrogen in various water bodies such as surface water is aggravated, and the exceeding of the concentration of ammoniacal nitrogen and nitrate nitrogen seriously harms the quality and health of ecological environment. How to effectively remove the inorganic nitrogen pollution in the polluted water body has important practical significance.
The method for removing inorganic nitrogen pollutants in sewage by using microorganisms (biological denitrification for short) is an important technical method for reducing or eliminating nitrogen pollution of water bodies. The biological method mainly utilizes nitrification and denitrification metabolic pathways to remove ammonia nitrogen, nitrate nitrogen and nitrite nitrogen. Most of inorganic nitrogen pollutants in the sewage are converted into nitrogen gas to be removed from the water body through nitrification and denitrification. The biological denitrification method has the advantages of economy, feasibility, environmental friendliness and the like, and is the main process method for the denitrification treatment of the sewage at present.
Temperature is an important factor affecting the denitrification effect of microorganisms. The optimum temperature of the existing denitrifying microorganism strains such as nitrifying bacteria and denitrifying bacteria or the denitrifying process technology is mostly in the medium temperature range (25-35 ℃). When the temperature is reduced in autumn and winter, the activity and the quantity of the denitrification microorganisms are reduced, so that the denitrification efficiency and the denitrification effect of a sewage system are obviously reduced, and the quality of the effluent is unstable or does not reach the standard. Many researches in recent decades show that the application of the denitrification strain capable of tolerating low-temperature conditions has good potential in improving the denitrification effect of sewage. A large amount of active exploration is conducted by a plurality of research and development institutions and enterprise units at home and abroad around the contents of screening high-efficiency low-temperature-resistant denitrifying bacteria, improving the effect of a low-temperature denitrifying process and the like. However, many denitrogenating bacteria have poor denitrogenation capability and low denitrogenation efficiency under low temperature conditions (below 15 ℃). On the other hand, the existing low-temperature resistant denitrifying bacteria have few varieties and cannot better meet the requirement of high-efficiency denitrification treatment of complex polluted water bodies.
Disclosure of Invention
The invention aims to provide application of Rahnella aquatilis WS33 in sewage denitrification aiming at the current situations of poor effect at low temperature and insufficient low-temperature strain resources in the current sewage biological denitrification technology.
In order to achieve the purpose, the invention adopts the technical scheme that:
one Rahnella aquatilis WS33 strain which has been preserved in China general microbiological culture collection center in 2021, 8 months and 2 days, the preservation number of the strain is CGMCC No.22997, and the preservation address is as follows: xilu No. 1 Hospital No. 3, Beijing Chaoyang.
The 16S rRNA gene sequence of the strain WS33 has 99.5 percent of consistency with the 16S rRNA gene sequence (CP003244) of Rahnella aquatilis CIP 78.65 (T).
An application of Rahnella aquatica and an application of the strain WS33 in removing inorganic nitrogen in sewage.
The Rahnella aquatilis WS33 is applied to the removal of inorganic nitrogen in polluted water at the temperature of 6-30 ℃.
The strain is activated by using a liquid culture medium, and the culture conditions of shake flask fermentation are as follows: shaking at 80-180rpm at 20-35 deg.C for 24-48 hr with initial pH of the culture medium of 7.0-8.0, and adding into water containing inorganic nitrogen.
The activation medium comprises the following components: NaCl 1-10.0; peptone 0.5-10.0; 0.1-5.0 parts of yeast extract and 1000mL of distilled water, and the pH is adjusted to 7.0-8.0.
After the strain is activated, the strain is inoculated into the inorganic nitrogen polluted water body to be treated according to the inoculation amount of 0.5-10%.
The ammonia nitrogen concentration in the sewage is 0.5-100mg/L, the nitrate nitrogen concentration is 0.1-200mg/L, and the nitrite state concentration is 0.01-50 mg/L.
The salinity of the sewage (by NaCl concentration) is 0.1-30g/L, and the pH value of the sewage is 6.0-9.0. The invention has the beneficial effects that:
(1) the Rahnella aquatilis has good biological safety and high denitrification efficiency for low-concentration inorganic nitrogen pollution.
(2) The denitrification strain Rahnella aquatilis WS33 can be well adapted to low-temperature conditions, and can effectively remove ammoniacal nitrogen, nitrate nitrogen and nitrite nitrogen in a water body at 6 ℃;
(3) the strain of the invention keeps denitrification activity in a wide temperature range of 6-30 ℃, and can be used for denitrification treatment of sewage in geographical areas with large temperature difference change in northern China;
(4) the strain has good growth and reproduction capability under the condition of medium and low temperature, and the strain propagation and the seed liquid preparation are relatively easy, thereby being beneficial to industrial production and subsequent application.
Detailed Description
The present invention will be further described with reference to the following examples, but the present invention is not limited to the following examples.
The strain separating screen is selected from an activated sludge tank of a sewage treatment plant in Heilongjiang, is preliminarily identified as the laenna aquaticum, and has obvious degradation and removal effects on inorganic nitrogen in a water body under the low-temperature condition.
Materials required in the following examples:
1) activated sludge: collected from an activated sludge tank of a sewage treatment plant in Heilongjiang province.
2) Culture medium:
enrichment culture medium: 10.0 parts of NaCl; 10.0 parts of peptone; and 5.0 of yeast extract.
Denitrification Medium (g.L) -1 )：CH 3 COONa 1.54；NH 4 Cl 0.15、KNO 3 0.16、NaNO 2 0.14、KH 2 PO 4 0.044；CaCl 2 ·2H 2 O 0.2；MgSO 4 ·7H 2 O 0.1；FeSO 4 ·7H 2 O0.006; 1mL of trace element liquid; 1L of distilled water; the pH value is 7.0-7.5; solid medium was supplemented with 2% agar.
Microelement liquid (g/L): EDTA 0.35；ZnSO 4 ·7H 2 O 0.2；CuSO 4 ·5H 2 O 0.1； MnSO 4 ·7H 2 O 0.1；Co(NO 3 ) 2 ·7H 2 O 0.09；H 3 BO 3 0.1；Na 2 MoO 4 0.1。
Sterilizing the above culture medium in a sterilizing pot at 103.4kPa and 121 deg.C for 20min, and cooling.
3) An experimental instrument:
a constant temperature incubator, a constant temperature shaking incubator, an ultra-clean workbench, a vertical pressure steam sterilizer, a gel imaging system, a micro ultraviolet spectrophotometer, a PCR instrument, an electrophoresis apparatus, a vortex oscillator and the like.
Example 1
Screening, identifying and preserving strain WS33
Taking 15g of activated sludge, putting the activated sludge into a 250mL conical flask which is pre-filled with 100mL of enrichment medium, and then carrying out shake culture on a constant-temperature shaking table at 15 ℃ and 180rpm for 72 h;
after 72h, taking the upper layer bacteria liquid after standing, performing gradient dilution by 10 times, and coating the upper layer bacteria liquid on a denitrification culture medium flat plate;
after bacterial colonies on a screening culture medium grow, selecting single bacterial colonies by using an aseptic inoculating loop, inoculating the single bacterial colonies on a denitrification solid culture medium by adopting a plate streaking separation method, placing the single bacterial colonies in a 15 ℃ constant-temperature incubator for culturing for 24 hours, selecting the single bacterial colonies after the bacterial colonies grow out, repeating the streaking separation process until the bacterial colonies are single in shape, and measuring the denitrification effect to obtain the bacterial strain with the largest ammonia nitrogen removal rate, wherein the bacterial strain is numbered as WS 33.
Genomic DNA of the strain WS33 was extracted and sent to Beijing Liu-He Hua Dagen technology Co., Ltd for 16S rRNA gene sequencing. The sequence alignment preliminarily determines that the strain WS33 has the closest genetic relationship with Rahnella aquatilis. The 16S rRNA gene sequence of the strain is as follows:
TGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTC GAGCGG
CAGCGGAAAGTAGCTTGCTACTTTGCCGGCGAGCGGCGGACGGG TGAGTA
ATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAAC GGTAGC
TAATACCGCATGACCTCGAAAGAGCAAAGTGGGGGATCTTCGGA CCTCAC
GCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGAGGTAAT GGCTC
ACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCA CACTGG
AACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGG AATATTG
CACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTGTGAAG AAGGCC
TTAGGGTTGTAAAGCACTTTCAGCGAGGAGGAAGGCATCATACTT AATAC
GTGTGGTGATTGACGTTACTCGCAGAAGAAGCACCGGCTAACTC CGTGCC
AGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTA CTGGGC
GTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAATCCC CGCGCT
TAACGTGGGAACTGCATTTGAAACTGGCAAGCTAGAGTCTTGTA GAGGGG
GGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAG GAATAC
CGGTGGCGAAGGCGGCCCCCTGGACAAAGACTGACGCTCAGGT GCGAAAG
CGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCTGT AAACGA
TGTCGACTTGGAGGTTGTGCCCTTGAGGCGTGGCTTCCGGAGCT AACGCG
TTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTCA AATGAA
TTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCG ATGCAA
CGCGAAGAACCTTACCTACTCTTGACATCCACGGAATTCGCCAGA GATGG
CTTAGTGCCTTCGGGAACCGTGAGACAGGTGCTGCATGGCTGTC GTCAGC
TCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACC CTTATC
CTTTGTTGCCAGCACGTGATGGTGGGAACTCAAAGGAGACTGCC GGTGAT
AAACCGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTT ACGAGT
AGGGCTACACACGTGCTACAATGGCATATACAAAGAGAAGCGAA CTCGCG
AGAGCAAGCGGACCTCATAAAGTATGTCGTAGTCCGGATTGGAGT CTGCA
ACTCGACTCCATGAAGTCGGAATCGCTAGTAATCGTAGATCAGAA TGCTA
CGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCA TGGGA
GTGGGTTGCAA
the strain WS33 is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC No. 22997.
Example 2
WS33 strain was inoculated into a 250mL conical flask containing 100mL enriched medium and activated at 30 ℃ for 48h on a constant temperature shaker at 180 rpm. And after 48 hours, taking the bacterial liquid, inoculating the bacterial liquid into a denitrification culture medium according to the inoculation proportion of 1 wt%, and then culturing for 72 hours at constant temperature of 6 ℃ and 180 rpm. And inoculating WS33 bacterial liquid sterilized by high-temperature steam in the same volume as the inoculated group as a control group, wherein other culture conditions are the same as those of the inoculated group. And after the culture period is finished, taking a water sample to measure the concentrations of ammoniacal nitrogen, nitrate nitrogen and nitrite nitrogen. The results are shown in Table 1.
As can be seen from the measurement results in Table 1, after 72 hours, the degradation rates of ammoniacal nitrogen, nitrate nitrogen and nitrite nitrogen of the treatment group inoculated with the WS33 strain are respectively 51.5%, 48.3% and 32.4%; the degradation rates of ammoniacal nitrogen, nitrate nitrogen and nitrite nitrogen in a control water sample inoculated with the sterilized WS33 bacterial solution are respectively only 0.12%, 0.52% and 0.05%. The result shows that the denitrification effect of the inoculated WS33 is obvious under the low-temperature condition of 6 ℃.
Table 1 shows the denitrification effect of Rahnella aquatilis WS33 at 6 deg.C
Water sample numbering Ammonia nitrogen removal (%) Nitrate nitrogen removal (%) Nitrite nitrogen removal (%)
Inoculation ofWS33 51.5 48.3 32.4
Vaccination Sterilization WS33 0.12 0.52 0.05
Example 3
The activated WS33 bacterial liquid in example 2 was inoculated into denitrification medium at an inoculation ratio of 1 wt%, and then cultured at 10 ℃ and 180rpm for 72 h. And inoculating WS33 bacterial liquid sterilized by high-temperature steam in the same volume as the inoculated group as a control group, wherein other culture conditions are the same as those of the inoculated group. And after the culture period is finished, taking a water sample to measure the concentrations of ammoniacal nitrogen, nitrate nitrogen and nitrite nitrogen. The results are shown in Table 2.
As can be seen from the measurement results in Table 2, the degradation rates of ammoniacal nitrogen, nitrate nitrogen and nitrite nitrogen of the treatment group inoculated with the WS33 strain after 72 hours are 69.7%, 65.6% and 52.1% respectively; the degradation rates of ammoniacal nitrogen, nitrate nitrogen and nitrite nitrogen in a control water sample inoculated with the sterilized WS33 bacterial solution are respectively only 0.19%, 0.10% and 0.07%. The result shows that the denitrification effect of the inoculated WS33 is improved to a certain extent compared with that of the inoculated WS33 at the low temperature of 10 ℃, and the denitrification effect is obvious.
Table 2 shows the denitrification effect of Rahnella aquatilis WS33 at 10 deg.C
Water sample numbering Ammonia nitrogen removal (%) Nitrate nitrogen removal (%) Nitrite nitrogen removal (%)
Inoculation of WS33 69.7 65.6 52.1
Vaccination Sterilization WS33 0.19 0.10 0.07
Example 4
The activated WS33 bacterial liquid in example 2 was inoculated into denitrification medium at an inoculation ratio of 1 wt%, and then cultured at 15 ℃ and 180rpm for 72 h. And inoculating WS33 bacterial liquid sterilized by high-temperature steam in the same volume as the inoculated group as a control group, wherein other culture conditions are the same as those of the inoculated group. And after the culture period is finished, taking a water sample to measure the concentrations of ammoniacal nitrogen, nitrate nitrogen and nitrite nitrogen. The results are shown in Table 3.
As can be seen from the measurement results in Table 3, the degradation rates of ammoniacal nitrogen, nitrate nitrogen and nitrite nitrogen of the treatment group inoculated with the WS33 strain after 72 hours are 84.5%, 88.3% and 72.4% respectively; the degradation rates of ammoniacal nitrogen, nitrate nitrogen and nitrite nitrogen in a water sample of a control group inoculated with the sterilized WS33 bacterial liquid are respectively only 0.11%, 0.13% and 0.04%. The result shows that the denitrification effect of the inoculated WS33 is further improved and is obvious under the low-temperature condition of 15 ℃.
Table 3 shows the denitrification effect of Rahnella aquatilis WS33 at 15 ℃
Water sample numbering Ammonia nitrogen removal (%) Nitrate nitrogen removal (%) Nitrite nitrogen removal (%)
Inoculation of WS33 84.5 88.3 72.4
Vaccination Sterilization WS33 0.11 0.13 0.04
Example 5
The activated WS33 bacterial liquid in example 2 was inoculated into denitrification medium at an inoculation ratio of 1 wt%, and then cultured at 10 ℃ and 180rpm for 72 h. And inoculating WS33 bacterial liquid sterilized by high-temperature steam in the same volume as the inoculated group as a control group, wherein other culture conditions are the same as those of the inoculated group. And after the culture period is finished, taking a water sample to measure the concentrations of ammoniacal nitrogen, nitrate nitrogen and nitrite nitrogen. The results are shown in Table 4.
As can be seen from the measurement results in Table 4, the degradation rates of ammoniacal nitrogen, nitrate nitrogen and nitrite nitrogen of the treatment group inoculated with the WS33 strain after 72 hours are respectively 99.5%, 98.3% and 92.1%; the degradation rates of ammoniacal nitrogen, nitrate nitrogen and nitrite nitrogen in a control water sample inoculated with the sterilized WS33 bacterial solution are respectively only 0.23%, 0.06% and 0.01%. The results show that the WS33 inoculated with the strain has remarkable denitrification effect under the condition of 30 ℃.
Table 4 shows the denitrification effect of Rahnella aquatilis WS33 at 30 ℃
Water sample numbering Ammonia nitrogen removal (%) Nitrate nitrogen removal (%) Nitrite nitrogen removal (%)
Inoculation of WS33 99.5 98.3 92.1
Vaccination Sterilization WS33 0.23 0.06 0.01
Sequence listing
<110> Shenyang landscape architecture shares Ltd
<120> Laenhei aquatica and application thereof in sewage denitrification
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1411
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 1
tggctcagat tgaacgctgg cggcaggcct aacacatgca agtcgagcgg cagcggaaag 60
tagcttgcta ctttgccggc gagcggcgga cgggtgagta atgtctggga aactgcctga 120
tggaggggga taactactgg aaacggtagc taataccgca tgacctcgaa agagcaaagt 180
gggggatctt cggacctcac gccatcggat gtgcccagat gggattagct agtaggtgag 240
gtaatggctc acctaggcga cgatccctag ctggtctgag aggatgacca gccacactgg 300
aactgagaca cggtccagac tcctacggga ggcagcagtg gggaatattg cacaatgggc 360
gcaagcctga tgcagccatg ccgcgtgtgt gaagaaggcc ttagggttgt aaagcacttt 420
cagcgaggag gaaggcatca tacttaatac gtgtggtgat tgacgttact cgcagaagaa 480
gcaccggcta actccgtgcc agcagccgcg gtaatacgga gggtgcaagc gttaatcgga 540
attactgggc gtaaagcgca cgcaggcggt ttgttaagtc agatgtgaaa tccccgcgct 600
taacgtggga actgcatttg aaactggcaa gctagagtct tgtagagggg ggtagaattc 660
caggtgtagc ggtgaaatgc gtagagatct ggaggaatac cggtggcgaa ggcggccccc 720
tggacaaaga ctgacgctca ggtgcgaaag cgtggggagc aaacaggatt agataccctg 780
gtagtccacg ctgtaaacga tgtcgacttg gaggttgtgc ccttgaggcg tggcttccgg 840
agctaacgcg ttaagtcgac cgcctgggga gtacggccgc aaggttaaaa ctcaaatgaa 900
ttgacggggg cccgcacaag cggtggagca tgtggtttaa ttcgatgcaa cgcgaagaac 960
cttacctact cttgacatcc acggaattcg ccagagatgg cttagtgcct tcgggaaccg 1020
tgagacaggt gctgcatggc tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg 1080
caacgagcgc aacccttatc ctttgttgcc agcacgtgat ggtgggaact caaaggagac 1140
tgccggtgat aaaccggagg aaggtgggga tgacgtcaag tcatcatggc ccttacgagt 1200
agggctacac acgtgctaca atggcatata caaagagaag cgaactcgcg agagcaagcg 1260
gacctcataa agtatgtcgt agtccggatt ggagtctgca actcgactcc atgaagtcgg 1320
aatcgctagt aatcgtagat cagaatgcta cggtgaatac gttcccgggc cttgtacaca 1380
ccgcccgtca caccatggga gtgggttgca a 1411

Claims (6)

1. A Rahnella aquatilis is characterized in that: the Rahnella aquatilis WS33 strain has been deposited in China general microbiological culture Collection center (CGMCC) at 8/2/2021, and the preservation number of the strain is CGMCC No. 22997.
2. The use of the strain WS33 for removing inorganic nitrogen from sewage, as claimed in claim 1.
3. Use according to claim 2, wherein said Rahnella aquatilis WS33 is used for removing inorganic nitrogen from contaminated water bodies at 6-30 ℃.
4. The use according to claim 4, wherein said strain is activated using a liquid medium under the following conditions: shaking at 80-180rpm at 20-35 deg.C for 24-48 hr with initial pH of culture medium of 7.0-8.0, and adding the activated strain into the water body containing inorganic nitrogen.
5. The use according to claim 2, characterized in that the ammoniacal nitrogen concentration in the sewage is 0.5-100mg/L, the nitrate nitrogen concentration is 0.1-200mg/L, and the nitrite concentration is 0.01-50 mg/L.
6. Use according to claim 2, wherein the effluent has a salinity (in NaCl concentration) of 0.1-30g/L and a pH of 6.0-9.0.
CN202210599246.XA 2022-05-30 2022-05-30 Rahnella aquatilis and application thereof in sewage denitrification Pending CN114874946A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202210599246.XA CN114874946A (en) 2022-05-30 2022-05-30 Rahnella aquatilis and application thereof in sewage denitrification

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202210599246.XA CN114874946A (en) 2022-05-30 2022-05-30 Rahnella aquatilis and application thereof in sewage denitrification

Publications (1)

Publication Number Publication Date
CN114874946A true CN114874946A (en) 2022-08-09

Family

ID=82680160

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202210599246.XA Pending CN114874946A (en) 2022-05-30 2022-05-30 Rahnella aquatilis and application thereof in sewage denitrification

Country Status (1)

Country Link
CN (1) CN114874946A (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110964663A (en) * 2019-12-04 2020-04-07 北京化工大学 Heterotrophic nitrifying bacteria for low-temperature sewage denitrification and application thereof
CN111117923A (en) * 2020-01-13 2020-05-08 常州大学 New Laenibacillus aquaticus strain Gj-4 with pectin degrading function
AU2019303431B1 (en) * 2019-01-15 2020-05-14 Agro-environmental Protection Institute, Ministry Of Agriculture And Rural Affairs LAS-degrading and/or N-removing bacterium and application thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2019303431B1 (en) * 2019-01-15 2020-05-14 Agro-environmental Protection Institute, Ministry Of Agriculture And Rural Affairs LAS-degrading and/or N-removing bacterium and application thereof
CN110964663A (en) * 2019-12-04 2020-04-07 北京化工大学 Heterotrophic nitrifying bacteria for low-temperature sewage denitrification and application thereof
CN111117923A (en) * 2020-01-13 2020-05-08 常州大学 New Laenibacillus aquaticus strain Gj-4 with pectin degrading function

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
KIL YONG KIM ET AL.: ""Rahnella aquatilis, a bacterium isolated from soybean rhizosphere, can solubilize hydroxyapatite"", 《FEMS MICROBIOLOGY LETTERS》, vol. 153, pages 273 - 277 *
梁靖 等: ""水生拉恩菌研究进展"", 《水产科学》, vol. 39, no. 6, pages 972 - 978 *

Similar Documents

Publication Publication Date Title
CN111733113B (en) COD (chemical oxygen demand) degrading strain and application thereof
CN104357035B (en) Biological bactericide for preventing and controlling SRB (sulfate reducing bacteria) in high-temperature water body and SRB inhibition method of bactericide
CN109456926A (en) A kind of microbial bacterial agent and its application containing thermophilic salt denitrifying bacterium YL5-2
CN110283739A (en) The denitrifying bacteria of one plant of salt tolerant and its application
CN114890555B (en) Solid microbial preparation for treating rural black and odorous water body and preparation method and application thereof
CN102268387B (en) Ammonia oxidizing bacteria, and separation method and application thereof
CN110846254A (en) Compound microbial agent for denitrification and preparation method and application thereof
CN104312957A (en) Denitrifying bacterium and fermenting production method thereof
CN113462622A (en) Pseudomonas for efficiently degrading various aromatic pollutants and application thereof
CN104480045A (en) Efficient aerobic denitrifying strain having heterotrophic nitrification function and application of strain
CN114292798B (en) Anaerobic denitrifying strain and application thereof in riverway water body remediation
CN113583897B (en) Bacillus aryabhattai FL05 and application thereof
CN110157637A (en) Enterobacteria Z1 and klebsiella Z2 composite bacteria agent removal high nitrogen pollutant effluents and application
CN113583918B (en) River sediment degrading strain and application thereof
CN114874946A (en) Rahnella aquatilis and application thereof in sewage denitrification
CN113698047A (en) Method for purifying rural domestic sewage by microorganism-enhanced artificial wetland
CN110643554B (en) Strain capable of efficiently removing inorganic phosphorus in water body and application thereof
CN114836355B (en) Pseudomonas DT04 and application thereof in sewage denitrification
CN110734878A (en) bacterial strain separation method for high ammonia nitrogen resistant HN-AD
CN114854641A (en) Low-temperature-resistant composite denitrifying bacteria and application thereof in sewage treatment
CN112920958B (en) High-concentration wastewater biological pretreatment functional bacteria and application thereof
CN115851516B (en) High-temperature-resistant high-salt aerobic denitrifying bacterium, and culture method and application thereof
CN115558625B (en) Low-temperature denitrification revolve pseudomonas strain and application thereof
CN113684159B (en) Palyra barbadensis and application thereof in denitrification and dephosphorization of river water body
CN114317382B (en) Anaerobic strain applied to riverway water body COD degradation and application thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination