CN114868842B - Coated nutritional feed additive for preventing anemia and promoting immune function of piglets and preparation method and application thereof - Google Patents
Coated nutritional feed additive for preventing anemia and promoting immune function of piglets and preparation method and application thereof Download PDFInfo
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- CN114868842B CN114868842B CN202210498287.XA CN202210498287A CN114868842B CN 114868842 B CN114868842 B CN 114868842B CN 202210498287 A CN202210498287 A CN 202210498287A CN 114868842 B CN114868842 B CN 114868842B
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Abstract
A coated nutritional feed additive for preventing piglet anemia and promoting immunity, and its preparation method and application are provided. The application belongs to the field of feed additives. The application aims to solve the technical problems that the piglets have high requirements on trace elements and low absorptivity and diarrhea is easy to cause by adding too high iron. The feed additive is prepared from silicon dioxide, lactic acid, fumaric acid, aminolevulinic acid, citric acid, vitamin B group, phosphorylated vitamin C, fish meal, glycine chelated iron, L-arginine-alpha-ketoglutarate, oligosaccharide, pyruvic acid creatine and rice bran meal. The additive of the application improves the immune function of the organism mainly by interfering three physiological functions of organism defense, balance and immune system, can obviously improve the daily gain of piglets, reduce the feed conversion ratio, effectively prevent the diarrhea phenomenon of the piglets and improve the economic value.
Description
Technical Field
The application belongs to the field of feed additives, and particularly relates to a coated nutritional feed additive for preventing piglet anemia and promoting immune function, and a preparation method and application thereof.
Background
Although primary piglet digestive organs are formed, the volume and weight of the primary piglet digestive organs are smaller and less developed, and under the condition that the development of the early piglet digestive tracts is not complete, how to promote the optimal digestion and absorption capacity of the early piglet, improve the immunity of the piglet, ensure the nutritional needs of the piglet, smoothly pass weaning stress and dig the maximum growth potential is the current primary problem.
The weight and blood erythrocyte volume of the piglets are rapidly increased at this stage due to insufficient ingestion and nutrition deficiency, so that if the iron required in the body cannot be timely and effectively supplemented, slow growth, reduced feed efficiency and reduced immunity can be caused, and even death of the piglets can be caused when the iron is severe. In order to meet the iron demand of piglets in the current feed, an additional iron source is often added into daily ration. However, the piglets have high trace element requirements and low absorptivity, and the addition of too high iron increases the reproduction of harmful bacteria in the intestinal tracts of the piglets, so that the diarrhea rate is increased, and the additional iron source and the rational application are more required.
Disclosure of Invention
The application aims to solve the technical problems that the requirement of piglets on trace elements is large, the absorption rate is low and diarrhea is easy to cause by adding too high iron, and provides a coated nutritional feed additive for preventing anemia and promoting immune function of piglets, and a preparation method and application thereof.
The application relates to a coated nutritional feed additive for preventing piglet anemia and promoting immune function, which is prepared from, by mass, 19.0-19.3 parts of silicon dioxide, 27.0-27.5 parts of lactic acid, 1.50-2.50 parts of fumaric acid, 0.80-1.20 parts of aminolevulinic acid, 0.80-1.20 parts of citric acid, 0.04-0.05 part of vitamin B group, 0.40-0.60 part of phosphorylated vitamin C, 4.50-5.50 parts of fish meal, 1.50-2.50 parts of glycine chelated iron, 6.50-7.50 parts of L-arginine-alpha-ketoglutarate, 9.0-11.0 parts of oligosaccharide, 90-110 parts of pyruvic acid and 800-850 parts of rice bran meal.
Further defined, the vitamin B group is one or a mixture of several of vitamin B6, vitamin B1 and vitamin B12 according to any ratio.
Further defined, the oligosaccharide is one or a mixture of a plurality of chitosan oligosaccharide, fructooligosaccharide, mannooligosaccharide and xylooligosaccharide according to any ratio.
The preparation method of the coated nutritional feed additive for preventing piglet anemia and promoting immune function comprises the following steps:
step 1: mixing silicon dioxide with lactic acid, adding pulverized and sieved fumaric acid, aminolevulinic acid and citric acid, continuously mixing, adding povidone solution, sequentially extruding, rounding, granulating, drying and sieving to obtain pellets, and coating the pellets in a boiling fluidized bed to obtain coated particles;
step 2: after crushing rice bran meal, firstly putting a part of crushed rice bran meal into a mixer, then adding vitamin B group, phosphorylated vitamin C and fish meal after preliminary screening for mixing, after mixing uniformly, adding another part of rice bran meal, glycine chelated iron, L-arginine alpha-ketoglutarate and oligosaccharide for continuous mixing, after mixing uniformly, adding the rest rice bran meal, pyruvic acid creatine and the coated particles obtained in the step 1, and continuously mixing until uniformity, thus obtaining the coated nutritional feed additive for preventing piglet anemia and promoting immune function.
Further defined, the pellet size in step 1 is 35-45 mesh.
Further defined, the ratio of the sum of the masses of silica, lactic acid, fumaric acid, aminolevulinic acid, citric acid to the volume of povidone solution in step 1 is 7mg:3mL.
Further defined, the coating in step 1 is a coating with an ethanol solution of polyacrylic resin.
Further defined, the polyacrylic resin is one or two of polyacrylic resin II and polyacrylic resin IV.
Further defined, the mass concentration of the polyacrylic resin in the ethanol solution of the polyacrylic resin is 2-7%.
Further defined, the part of the crushed rice bran meal in the step 2 is 30% -50% of the total mass of the rice bran meal.
Further defined, the other part of the rice bran meal in the step 2 is 30% -50% of the total mass of the rice bran meal.
Further defined, the preliminary screening in step 2 means screening through a 40 mesh screen.
Further defined, the mixing in step 2 is performed by using a three-dimensional mixer.
Further limiting, adding vitamin B group, phospho-esterified vitamin C and the fish meal after preliminary screening in the step 2, mixing for 15-20 min, adding another part of rice bran meal, glycine chelated iron, L-arginine alpha-ketoglutarate and oligosaccharide, continuously mixing for 15-20 min, adding the rest rice bran meal, pyruvic acid creatine and the coated particles obtained in the step 1, and continuously mixing for 15-20 min.
The coated nutritional feed additive for preventing piglet anemia and promoting immune function is applied as a feed additive, and the additive amount of the coated nutritional feed additive in the feed is 0.8-1.2 per mill.
Compared with the prior art, the application has the following advantages:
1) The additive of the application improves the immune function of the organism mainly by interfering three physiological functions of organism defense, balance and immune system, firstly, thymus is the most important immune organ for early stage of piglet, and both aminolevulinic acid and arginine can increase the weight of thymus and promote the growth of thymus cells and T cells so as to play an excellent role in improving the immune function.
2) Creatine can obviously improve the normal performance of mitochondrial functions, pyruvic acid reduces the generation of free radicals of liver cells and reduces the oxidative damage of the liver cells, but independent pyruvic acid is relatively active and is not easy to be added independently, and the pyruvic acid creatine can have the dual physiological functions of pyruvic acid and creatine and is easy to be added and stored.
3) The alpha-ketoglutaric acid also has a certain antioxidation effect, and the alpha-ketoglutaric acid has a certain effect on scavenging oxygen free radicals, thereby being beneficial to enhancing the antioxidation capability of piglets. The alpha-ketoglutarate can relieve the increase of the content of malondialdehyde in the liver and the decrease of the activity of superoxide dismutase, and reduce the oxidation injury resistance of the liver of the piglet, and the addition of the alpha-ketoglutarate in the ration of the piglet can protect the normal function of the liver.
4) According to the application, the citric acid and the fumaric acid promote the propagation of beneficial intestinal bacteria, inhibit the growth of harmful bacteria, improve the quantity of lactobacillus in jejunum and cecum, reduce the quantity of escherichia coli, reduce the occurrence of diarrhea of piglets, effectively prevent diarrhea of piglets and improve the survival rate of piglets.
5) The amino levulinic acid, the vitamin C and the glycine chelated iron are combined in the additive, and through activating blood cell and myoglobin in an animal body, the oxygen transmission in the organism, the nutrient transmission and the metabolism efficiency are improved, the utilization rate of iron elements and other nutrient elements in the feed is further improved, the absorption rate and the metabolism speed are increased, and the additive has remarkable physiological effects. The fumaric acid in the additive can promote mineral element Ca 2+ And Zn 2+ Is absorbed and accumulated. The fumaric acid and the high-level copper sulfate in the feed have synergistic effect, and the utilization rate of the feed can be improved by using the fumaric acid and the high-level copper sulfate together.
6) The additive disclosed by the application is used by compounding acidic diet, vitamin C and other reducing substances to promote the absorption of iron, and the additive still keeps a soluble state in small intestine with reduced acidity, so that the iron is facilitated to enter plasma through the small intestine, in addition, the total iron retention rate of glycine chelate iron in the additive in an aqueous solution is kept at about 98%, and the catalytic oxidation effect of grease is very small.
7) The coated acidulant can be released in a segmented way, so that the defects that the common acidulant is excessively absorbed in the stomach and cannot reach the intestinal tract are overcome, and the coated product has more stable quality, no corrosiveness and no hygroscopicity; other components in the feed are not destroyed, and the feed is easy to flow and uniformly mixed.
Detailed Description
Example 1: the coated nutritional feed additive for preventing piglet anemia and promoting immune function is prepared from 19.12 parts of silicon dioxide, 27.36 parts of lactic acid, 2.00 parts of fumaric acid, 1.00 parts of aminolevulinic acid, 1.00 parts of citric acid, 60.02 parts of vitamin B, 10.025 parts of vitamin B120.0002 parts of vitamin B, 0.50 part of phosphorylated vitamin C, 5.00 parts of fish meal, 2.00 parts of glycine chelated iron, 7.00 parts of L-arginine-alpha-ketoglutarate, 2.00 parts of oligomeric chitosan, 2.00 parts of fructo-oligosaccharide, 1.00 parts of mannooligosaccharide, 5.00 parts of xylooligosaccharide, 100 parts of pyruvic acid and 825 parts of rice bran meal.
The preparation method of the coated nutritional feed additive for preventing piglet anemia and promoting immune function of the embodiment 1 comprises the following steps:
step 1: mixing silicon dioxide and lactic acid for 5min, adding pulverized and sieved fumaric acid (40 meshes), aminolevulinic acid (40 meshes) and citric acid (40 meshes) for continuous mixing, adding 2.15mL povidone k30 solution, sequentially extruding, rounding, granulating, drying, sieving with a 40-mesh sieve to obtain pellets, and coating the pellets in a boiling fluidized bed to obtain coated particles; the coating is carried out by adopting an ethanol solution of polyacrylic resin II with the concentration of 5%, wherein the mass concentration of the ethanol is 95%;
step 2: crushing rice bran meal, putting 40wt% of crushed rice bran meal into a three-dimensional mixer, adding vitamin B group, phosphorylated vitamin C and fish meal subjected to primary screening, mixing for 20min, adding 30wt% of rice bran meal, glycine chelated iron, L-arginine alpha-ketoglutarate and oligosaccharide, continuously mixing for 20min, adding the rest rice bran meal, pyruvic acid creatine and the coated particles obtained in the step 1 after uniformly mixing, and continuously mixing for 20min to be uniform, thus obtaining the coated nutritional feed additive for preventing piglet anemia and promoting immune function.
Example 2: the coated nutritional feed additive for preventing piglet anemia and promoting immune function is prepared from 19.12 parts of silicon dioxide, 27.36 parts of lactic acid, 2.00 parts of fumaric acid, 1.00 parts of aminolevulinic acid, 1.00 parts of citric acid, 60.02 parts of vitamin B, 10.025 parts of vitamin B120.0002 parts of vitamin B, 0.50 part of phosphorylated vitamin C, 5.00 parts of fish meal, 2.00 parts of glycine chelated iron, 7.00 parts of L-arginine-alpha-ketoglutarate, 2.00 parts of oligomeric chitosan, 2.00 parts of fructo-oligosaccharide, 1.00 parts of mannooligosaccharide, 5.00 parts of xylooligosaccharide, 100 parts of pyruvic acid and 825 parts of rice bran meal.
The preparation method of the coated nutritional feed additive for preventing piglet anemia and promoting immune function of example 2 is carried out according to the following steps:
step 1: mixing silicon dioxide and lactic acid for 5min, adding pulverized and sieved fumaric acid (40 meshes), aminolevulinic acid (40 meshes) and citric acid (40 meshes) for continuous mixing, adding 2.15mL povidone k30 solution, sequentially extruding, rounding, granulating, drying, sieving with a 40-mesh sieve to obtain pellets, and coating the pellets in a boiling fluidized bed to obtain coated particles; the coating is carried out by adopting an ethanol solution of polyacrylic resin IV with the concentration of 5%, wherein the mass concentration of the ethanol is 95%;
step 2: crushing rice bran meal, putting 40wt% of crushed rice bran meal into a three-dimensional mixer, adding vitamin B group, phosphorylated vitamin C and fish meal subjected to primary screening, mixing for 20min, adding 30wt% of rice bran meal, glycine chelated iron, L-arginine alpha-ketoglutarate and oligosaccharide, continuously mixing for 20min, adding the rest rice bran meal, pyruvic acid creatine and the coated particles obtained in the step 1 after uniformly mixing, and continuously mixing for 20min to be uniform, thus obtaining the coated nutritional feed additive for preventing piglet anemia and promoting immune function.
Example 3: the coated nutritional feed additive for preventing piglet anemia and promoting immune function is prepared from 19.12 parts of silicon dioxide, 27.36 parts of lactic acid, 2.00 parts of fumaric acid, 1.00 parts of aminolevulinic acid, 1.00 parts of citric acid, 60.02 parts of vitamin B, 10.025 parts of vitamin B120.0002 parts of vitamin B, 0.50 part of phosphorylated vitamin C, 5.00 parts of fish meal, 2.00 parts of glycine chelated iron, 7.00 parts of L-arginine-alpha-ketoglutarate, 2.00 parts of oligomeric chitosan, 2.00 parts of fructo-oligosaccharide, 1.00 parts of mannooligosaccharide, 5.00 parts of xylooligosaccharide, 100 parts of pyruvic acid and 825 parts of rice bran meal.
The preparation method of the coated nutritional feed additive for preventing piglet anemia and promoting immune function of example 3 is carried out according to the following steps:
step 1: mixing silicon dioxide and lactic acid for 5min, adding pulverized and sieved fumaric acid (40 meshes), aminolevulinic acid (40 meshes) and citric acid (40 meshes) for continuous mixing, adding 2.15mL povidone k30 solution, sequentially extruding, rounding, granulating, drying, sieving with a 40-mesh sieve to obtain pellets, and coating the pellets in a boiling fluidized bed to obtain coated particles; the specific process of the coating is as follows: wherein 40% of the pellets are coated by adopting an ethanol solution of polyacrylic resin II with the concentration of 2%, 60% of the pellets are coated by adopting an ethanol solution of polyacrylic resin IV with the concentration of 5%, and the mass concentration of the ethanol is 95%;
step 2: crushing rice bran meal, putting 40wt% of crushed rice bran meal into a three-dimensional mixer, adding vitamin B group, phosphorylated vitamin C and fish meal subjected to primary screening, mixing for 20min, adding 30wt% of rice bran meal, glycine chelated iron, L-arginine alpha-ketoglutarate and oligosaccharide, continuously mixing for 20min, adding the rest rice bran meal, pyruvic acid creatine and the coated particles obtained in the step 1 after uniformly mixing, and continuously mixing for 20min to be uniform, thus obtaining the coated nutritional feed additive for preventing piglet anemia and promoting immune function.
Verification test: the coated nutritional additive of the application has effects on the growth performance of piglets in different growth stages, diarrhea prevention, immunity enhancement and piglet anemia prevention
Test one: the test procedure for 21-25 day old weaned pigs was as follows:
1. feed composition and nutrient level for the test
The determination of the content and the energy of the nutrient substances in the feed is carried out by referring to the feed analysis and feed quality detection technology which is mainly compiled by Yang Sheng. The feed formulation was formulated according to the NRC standard in 1998 and the agricultural industry standard pig raising standard in the people's republic of China (NY/T65-2004), and the test ration composition and nutrition level are shown in Table 1.
Table 1: feed composition and nutrient level
Note that: the premix in Table 1 provides Cu 5g, fe 130mg, zn 5g, mn 1.8g, se 15g, I20 mg, co 25mg, ca 160-270g, fe 4.6g, P38 g, water 10% 253, vitamin A (KIU) and vitamin D for each kg of compound feed 3 (KIU) is more than or equal to 47, vitamin E (IU) is more than or equal to 662, vitamin K is more than or equal to 53mg, and vitamin B 1 More than or equal to 58mg, vitamin B 2 More than or equal to 75mg of vitamin B 6 More than or equal to 67mg, vitamin B 12 More than or equal to 0.8mg, folic acid more than or equal to 27mg, biotin more than or equal to 1.1mg, nicotinic acid more than or equal to 975mg, calcium pantothenate more than or equal to 750mg, and choline chloride more than or equal to 5g.
2. Test animals and test groups
The test selects 72 binary (long x big) piglets which are strong in physique and well developed and weaned at 21-25 days, the piglets are randomly divided into 4 groups, the weight difference among the groups is not obvious (P > 0.05), each group is provided with 3 repeats, 6 repeats are respectively fed with the feed added with the nutrition additive of the embodiment 1 (shown in table 1), the test group II is fed with the feed added with the nutrition additive of the embodiment 2 (shown in table 1), the test group III is fed with the feed added with the nutrition additive of the embodiment 3 (shown in table 1), each test group is fed with 1kg of the nutrition additive of the embodiment in each ton of compound feed, the control group is only fed with the feed of table 1, and the pig farm in the north of the large farm group Tangshan Yufield is used.
3. Test piglet feeding management
The test piglets are all fed in the same pig house and managed by special persons, weaning time is 21 days, the pig house is strictly cleaned and disinfected 3 days before the piglets enter the pig house, daily ration of each group is fed for 7 days in a pre-feeding period, free feeding and free drinking are carried out for 10 days in a test period, normal immunity and insect expelling are carried out on the piglets, proper treatment is carried out on the sick piglets, the test piglets are fed by a high bed sheet, sufficient and clean drinking water is provided by a nipple-type automatic drinking machine, the temperature of the pig house is controlled at 22-24 ℃, free feeding is carried out, excrement is cleaned for 2 times every day, the inside of the pig house is kept clean, the growth and development of the piglets, feeding, diarrhea and other conditions of the piglets are observed at any time, and records are made.
4. Statistical analysis of test data
Experimental data statistics analysis of variance was performed using the balanced experimental design ANOVA procedure in SAS9.0 software package, with multiple comparisons of means using the Duncan method. Statistical analysis of economic benefit data was performed using Microsoft Excel in Office applications.
And (2) testing II: the test procedure for 40-day-old nursery stage piglets is as follows:
1. feed composition and nutrient level for the test
The determination of the content and the energy of the nutrient substances in the feed is carried out by referring to the feed analysis and feed quality detection technology which is mainly compiled by Yang Sheng. The feed formulation was formulated according to the NRC standard in 1998 and the agricultural industry standard pig raising standard in the people's republic of China (NY/T65-2004), and the test ration composition and nutrition level are shown in Table 2.
Table 2: feed composition and nutrient level
Note that: the premix in Table 2 provides Cu 5g, fe 130mg, zn 5g, mn 1.8g, se 15g, I20 mg, co 25mg, ca 160-270g, fe 4.6g, P38 g, water 10% 253, vitamin A (KIU) and vitamin D for each kg of compound feed 3 (KIU) is more than or equal to 47, vitamin E (IU) is more than or equal to 662, vitamin K is more than or equal to 53mg, and vitamin B 1 More than or equal to 58mg, vitamin B 2 More than or equal to 75mg of vitamin B 6 More than or equal to 67mg, vitamin B 12 More than or equal to 0.8mg, folic acid more than or equal to 27mg, biotin more than or equal to 1.1mg, nicotinic acid more than or equal to 975mg, calcium pantothenate more than or equal to 750mg, and choline chloride more than or equal to5g。
2. Test animals and test groups
The experiment selects 72 piglets of 40 days of healthy and strong and well developed nursing stage, which are randomly divided into 4 groups, the weight difference among the groups is not obvious (P > 0.05), each group is provided with 3 repeats, 6 repeats are respectively fed with feed added with the nutrition additive of the embodiment 1 (shown in table 2), the experiment V group is fed with feed added with the nutrition additive of the embodiment 2 (shown in table 2), the experiment VI group is fed with feed added with the nutrition additive of the embodiment 3 (shown in table 2), each experiment group is fed with 1kg of the nutrition additive of the embodiment in each ton of compound feed, the comparison group is only fed with the feed of table 2, and the experiment is carried out in the pig farm mountain Yufield of the northern area of the large farm group.
3. Test piglet feeding management
The test piglets are all fed in the same pig house and managed by special persons, weaning time is 21 days, the pig house is strictly cleaned and disinfected 3 days before the piglets enter the pig house, daily ration of each group is fed for 7 days in a pre-feeding period, free feeding and free drinking are carried out for 10 days in a test period, normal immunity and insect expelling are carried out on the piglets, proper treatment is carried out on the sick piglets, the test piglets are fed by a high bed sheet, sufficient and clean drinking water is provided by a nipple-type automatic drinking machine, the temperature of the pig house is controlled at 20-22 ℃, free feeding is carried out, excrement is cleaned for 2 times every day, the inside of the pig house is kept clean, the growth and development of the piglets, feeding, diarrhea and other conditions of the piglets are observed at any time, and records are made.
4. Statistical analysis of test data
Experimental data statistics analysis of variance was performed using the balanced experimental design ANOVA procedure in SAS9.0 software package, with multiple comparisons of means using the Duncan method. Statistical analysis of economic benefit data was performed using Microsoft Excel in Office applications.
And (3) test III: the test procedure for the 65-70 day old piglets is as follows:
1. feed composition and nutrient level for the test
The determination of the content and the energy of the nutrient substances in the feed is carried out by referring to the feed analysis and feed quality detection technology which is mainly compiled by Yang Sheng. The feed formulation was formulated according to the NRC standard in 1998 and the agricultural industry standard pig raising standard in the people's republic of China (NY/T65-2004), and the test ration composition and nutrition level are shown in Table 3.
Table 3: feed composition and nutrient level
Note that: the premix in Table 3 provides 3 g/kg of compound feed with Cu, 100 mg/kg of Fe, 3 g/kg of Zn, 1.8 g/kg of Mn, 15 g/kg of Se, 20 mg/kg of I, 25 mg/kg of Co, 120-270 g/kg of Ca, 4.6 g/kg of Fe, 30 g/kg of P, 10 g/kg of water, 253/kg of vitamin A (KIU) and D 3 (KIU) is more than or equal to 47, vitamin E (IU) is more than or equal to 662, vitamin K is more than or equal to 53mg, and vitamin B 1 More than or equal to 58mg, vitamin B 2 More than or equal to 75mg of vitamin B 6 More than or equal to 67mg, vitamin B 12 More than or equal to 0.8mg, folic acid more than or equal to 27mg, biotin more than or equal to 1.1mg, nicotinic acid more than or equal to 975mg, calcium pantothenate more than or equal to 750mg, and choline chloride more than or equal to 5g.
2. Test animals and test groups
The test selects 72 binary (long x big) piglets which are strong in physique and well developed and are 65-70 days old and have a weight of 28-30kg, the piglets are randomly divided into 4 groups, the weight difference among the groups is not obvious (P > 0.05), 3 repeats are arranged in each group, 6 repeats are arranged in each group, the feed added with the nutrition additive of the embodiment 1 is fed to the group VII (shown in table 3), the feed added with the nutrition additive of the embodiment 2 is fed to the group VIII (shown in table 3), the nutrition additive of the embodiment 3 is fed to the group IX (shown in table 3), 1kg of the nutrition additive of the embodiment is added to each ton of compound feed, the feed of the table 3 is fed to the group VI, and the pig farm is located in the Dazhong mountain and Yutian pig farm.
3. Test piglet feeding management
The test piglets are all fed in the same pig house and managed by special persons, weaning time is 21 days, the pig house is strictly cleaned and disinfected 3 days before the piglets enter the pig house, daily ration of each group is fed for 7 days in a pre-feeding period, free feeding and free drinking are carried out on the piglets in a test period, normal immunity and insect expelling are carried out on the piglets, proper treatment is carried out on the sick piglets, the test piglets are fed by a high bed sheet, sufficient and clean drinking water is provided by a nipple-type automatic drinking machine, the temperature of the pig house is controlled to be 16-20 ℃, free feeding is carried out, excrement is cleaned for 2 times every day, the inside of the pig house is kept clean, the growth and development of the piglets, feeding, diarrhea and other conditions of the piglets are observed at any time, and records are made.
4. Statistical analysis of test data
Experimental data statistics analysis of variance was performed using the balanced experimental design ANOVA procedure in SAS9.0 software package, with multiple comparisons of means using the Duncan method. Statistical analysis of economic benefit data was performed using Microsoft Excel in Office applications.
(II) test results and analysis
1. Effect of additives on piglet ADFI, ADG and F: G (feed to meat ratio) and diarrhea Rate
The first and last morning of the formal test period are subjected to fasting weighing, the material consumption and the diarrhea frequency are recorded, and the economic benefits of daily feed intake (ADFI), daily weight gain (ADG), material weight ratio (F: G), diarrhea rate and piglet weight gain are calculated by the following calculation method:
the results of test one are shown in Table 4, and as can be seen from Table 4: the additive can improve the growth performance of piglets to a certain extent, reduce diarrhea of piglets, and the test I and II groups are obviously higher than the control group and the test III group (P is less than 0.05) in terms of ADFI; there was no significant difference (P > 0.05) between the remaining groups. In terms of ADG, the additive test groups were all higher than the control group, and test group i was the highest. In terms of F and G, the test group I performed best, with each test group being lower than the control group; the diarrhea rate is optimal in the test group II, the effect of the control group is extremely poor, and the effects of other test groups are better.
TABLE 4 Effect of additives on piglet growth performance and diarrhea Rate
Note that: the same row of lower-case letters have different meanings, which indicates that the difference is significant (P < 0.05);
the results of the second test are shown in Table 5, and as can be seen from Table 5: the additive used in the test can improve the growth performance of the nursing piglets to a certain extent and reduce diarrhea of the nursing piglets. In terms of ADFI, the test group V is significantly higher than the control, IV, VI (P < 0.05); the groups IV and VI were significantly higher than the control group, but the differences between the groups IV and VI were not significant (P > 0.05). In terms of ADG, the additive test groups were all higher than the control group, and the test iv group was the highest. In terms of F to G, test group IV performed best; the diarrhea rate is best represented by the test VI group, the control group has extremely poor effect, and other test groups have better effect.
TABLE 5 influence of additives on the growth performance and diarrhea rate of nursery piglets
Note that: the same row of lower-case letters have different meanings, which indicates that the difference is significant (P < 0.05);
the results of the test three are shown in Table 6, and as can be seen from Table 6: the additive used in the test can improve the growth performance of piglets to a certain extent and reduce diarrhea of piglets. In terms of ADFI, the test group VIII is significantly higher than the control group, test groups VII, IX (P < 0.05); the test VII, IX groups were significantly higher than the control group, but the differences between the test VII, IX groups were not significant (P > 0.05). In terms of ADG, the additive test group was higher than the control group, and the test vii group was highest. In terms of F to G, the test VII group performed best; the diarrhea rate is best represented by the test VII group, the control group has poor effect, and other test groups have good effects.
TABLE 6 Effect of additives on piglet growth performance and diarrhea Rate
Note that: the same row of lower-case letters have different meanings, which indicates that the difference is significant (P < 0.05);
2. effect of additives on piglet immunity
2.1 measurement of T-lymphocyte conversion
(1) Blood sample collection and preparation
On the last day of the formal test period, all piglets for test were collected 2ml in the early morning on an empty stomach, placed in a test tube with ACD anticoagulation, and kept at 4℃for further use.
(2) Measurement method
The lymphocyte transformation test adopts a morphological examination method, uses non-specific mitogen Phytohemagglutinin (PHA) as a stimulator, takes 0.1mL of anticoagulated blood to be directly added into a lymphocyte transformation tube filled with 1mL of PMI 1640 culture solution (RPMI 1640 culture medium, fetal calf serum 20%, penicillin 100U/mL and PHA 50 mug/mL), plugs a tube mouth by using a rubber plug, shakes uniformly, cultures at 37 ℃ for 72 hours, shakes for 2 times every day, after stopping the culture, centrifugates (1000 r/min, 5-10 min), removes supernatant to disperse cells, drops the cells on a glass slide, pushes the cells away by the front end of a dropper to be uniformly distributed, and is subjected to Rate staining after drying, 200 lymphocytes are counted by observing by an oil microscope, and the transformation percentage is calculated according to the following formula:
as shown in Table 7, it is clear from Table 7 that the additive used in the test can increase LTR by 4.23%, 4.79% and 1.38% respectively, and that the test groups I and II are significantly higher than the control group and III (P < 0.05).
TABLE 7 Effect of additives on piglet blood lymphocyte conversion
Note that: the same row of lower-case letters are different, indicating significant differences (P < 0.05).
The results of the second test are shown in Table 8, and it is clear from Table 8 that the additive used in the test can improve the LTR of the nursery pigs, and the test IV and V groups are significantly higher than the control group and the test VI group (P < 0.05).
TABLE 8 Effect of additives on the conversion of blood lymphocytes from nursery piglets
Note that: the same row of lower-case letters are different, indicating significant differences (P < 0.05).
The results of the test three are shown in Table 9, and it is clear from Table 9 that there was no significant difference between each of the test groups and the control group, but it was found that there was a significant tendency to increase in the test VII group.
TABLE 9 Effect of additives on piglet blood lymphocyte conversion
2.2, serum IgG, igM, igA and complementC 3 、C 4 Is (are) determined by
(1) Blood sample collection and preparation
On the last day of the formal test period, all test piglets are subjected to blood sampling of 5ml in the early morning on an empty stomach, kept stand at room temperature for half an hour, centrifuged for 10min at 2000-2500r/min after serum is separated out, and the prepared serum sample is preserved at-20 ℃ for later use.
(2) Measurement index and method
The measurement index mainly comprises IgG, igM, igA and complement C in serum 3 、C 4 The kit for testing is purchased from Harbin irinotecan biotechnology Co., ltd, and the immunoglobulin IgG, IGM, IGA, C, C4 testing kit, and specific test parameters and operation steps are referred to the kit specifications.
(3) Statistical analysis of test data
Experimental data statistics analysis of variance was performed using the balanced experimental design ANOVA procedure in SAS9.0 software package, with multiple comparisons of means using the Duncan method.
The results of test one are shown in Table 10 and Table 11, and can be seen from Table 10: the additive used in the test can improve the serum immunoglobulin content of the piglets in a certain range, and in terms of IgG, the test group is obviously higher than the control group (P is less than 0.05), so that the test group II has the best effect; in IgM, each test group was significantly higher than the control group (P < 0.05), with best results in test group II, and test groups I and II were significantly higher than test group III (P < 0.05); igA groups were significantly higher than the control group (P < 0.05) with best results in group I and group II being significantly higher than group III (P < 0.05); as can be seen from Table 11, the additives used in the test increased serum complement levels and the test groups I and II performed better in terms of complement C3. In terms of complement C4, runs I and II performed better (P < 0.05); there was no significant difference (P > 0.05) between the control group and the test III group.
Table 10 effect of additives on serum immune proteins in piglets
Note that: the same row of lower-case letters have different meanings, which indicates that the difference is significant (P < 0.05);
table 11 Effect of additives on serum complement content in piglets
Note that: the same row of lower-case letters have different meanings, which indicates that the difference is significant (P < 0.05);
the results of test two are shown in Table 12 and Table 13, and can be seen from Table 12: the additive used in the test can improve the serum immunoglobulin content of the piglets in a certain range, and in terms of IgG, the test group is obviously higher than the control group (P is less than 0.05), so that the test VI has the best effect; in the aspect of IgM, each test group is higher than the control group (P is less than 0.05), the best effect is obtained in the test group IV, and the best effect is obtained in the test group IV; the IgG, igM, igA content in serum is the highest in test group IV, and the effect is best. As can be seen from table 13: the additive used in the test can improve the serum complement content, and in the aspect of complement C3, the test IV and V groups have better performance. In the aspect of complement C4, the test IV and V groups perform better (P < 0.05); there was no significant difference (P > 0.05) between the control and test VI groups.
Table 12 effect of additives on serum immune proteins of nursery piglets
Note that: the same row of lower-case letters have different meanings, which indicates that the difference is significant (P < 0.05);
TABLE 13 Effect of additives on serum complement content of nursery piglets
Note that: the same row of lower-case letters have different meanings, which indicates that the difference is significant (P < 0.05);
the results of test three are shown in tables 14 and 15, and can be seen from table 14: the additive used in the test can improve the serum immunoglobulin content of piglets in a certain range, and in terms of IgG, the test group VIII is obviously higher than the control group (P is less than 0.05), and the difference among the other groups is not obvious, so that the test group VIII has the best effect; the differences among groups are not significant in IgM, but the best effect is obtained in the test VII group; as can be seen from table 15: the additive used in the test can improve the serum complement content, and the test VII group has better performance in terms of complement C3 and the test VII and VIII groups have better performance in terms of complement C4; but there was no significant difference between the groups (P > 0.05).
Table 14 effect of additives on serum immune proteins in piglets
Note that: the same row of lower-case letters have different meanings, which indicates that the difference is significant (P < 0.05);
table 15 effect of additives on serum complement content in piglets
Note that: the same row of lower-case letters have different meanings, which indicates that the difference is significant (P < 0.05);
2.3 influence of additives on the effect of piglets on preventing anemia
(1) Test materials
Hydrogen peroxide, 0.1mol/LHN0 3 Centrifuge, 10mL centrifuge tube, blood cell analyzer, refrigerator, autoclave freezer, high-speed pulverizer, atomic absorption analyzer, microwave digester, microwave cleaner, acetylene gas, air compressor, electronic balance, blast drying oven, pulverizer, digester, volumetric flask, pipette, ceramic knife.
(2) Sample collection and processing
After the test is finished, selecting 10mL of anticoagulated blood (heparin sodium) from veins of 2 healthy piglets in each column, separating the rest blood for 15 minutes at 3000 rpm, and then sub-packaging the blood plasma into 1mL Eddindorf tubes for preservation in a refrigerator at the temperature of minus 80 ℃. And (3) tissue sampling and treatment, namely slaughtering 4 piglets with similar growth conditions after 10 days of test, putting the piglets into a plastic bag, freezing and preserving the piglets in a refrigerator at the temperature of minus 20 ℃, peeling off tissue skin, cutting out the cortex part of the tissue by a ceramic knife, putting the tissue into a culture dish, freeze-drying for 24 hours, crushing the tissue by a crusher, sieving the crushed tissue, and putting the crushed tissue into the plastic bag, and freezing and preserving the tissue in the refrigerator at the temperature of minus 20 ℃.
The feces collection and treatment comprises collecting about 100g of piglet feed, sealing in a sealing bag, returning to test, pulverizing, sieving, and freezing in a refrigerator at-20deg.C; collecting fresh manure sample in the unit of column at 7:30-8:00 am on test day 10, taking about 100g, placing into a sealing bag for sealing and preserving, returning to a laboratory, drying in a forced air drying oven at 65 ℃, pulverizing, sieving, and freezing in a refrigerator at-20deg.C. The preparation of anticoagulant, namely, preparing 1000IU/ml heparin sodium anticoagulant with 0.9% physiological saline, taking 10u1 for injection into a centrifuge tube one day before blood sampling, and drying at 100 ℃.
(3) Determination of iron content in piglet tissue and feces
Preparation of standard working solution by sucking 5mL of 1000ug/mL Fe standard solution into 50mL volumetric flask, and adding 0.1mol/LHNO 3 The standard intermediate solution with the concentration of 100mg/L is obtained by constant volume, and the standard intermediate solution with the concentration of 100mg/L is respectively absorbed into a 50mL volumetric flask by 0mL, 0.25mL, 0.5mL, 1.0mL, 1.5mL, 2mL, 2.5mL and 3mL, and the standard intermediate solution with the concentration of 0.lmol/mL HNO is respectively absorbed into the flask 3 To determine the iron content of the tissue, the standard solutions required by 0mL, 0.5mL, 1.0mL, 2.0mL, 3.0mL, 4.0mL, 5.0mL and 6.0mg/mL are prepared for constant volume. 1mL, 2mL, 3mL, 4mL, 5mL of standard intermediate was measured in a 50mL volumetric flask with 0.lmol/mL HNO 3 And (3) determining the iron content in the feces by fixing the volume and preparing standard solutions required by 2mg/mL, 4mg/mL, 6mg/mL, 8mg/mL and 10 mg/mL.
The sample weighing is that an analytical balance accurately weighs about 0.5g of feed, about 0.3g of spleen and about 0.2g of manure.
Sample digestion, namely weighing the sample, putting the sample into a digestion tube, respectively adding 4mL of nitric acid (industrial analytical grade), 1mL of hydrogen peroxide and 5mL of water, putting the mixture into a microwave digestion oven for digestion, and performing corresponding blank comparison when the sample is digested each time.
The solution is fixed in volume, the digested sample is transferred to a 50mL volumetric flask, and filtered and transferred to a 50mL test tube for preservation at-4 ℃.
And (3) measuring iron element in the sample, introducing an iron standard solution into an atomizer, drawing a standard curve, verifying the correlation coefficient of the standard curve, and sequentially introducing a blank solution and all test vessels of the sample according to prompts under the condition that the correlation coefficient is 99%, wherein the blank solution and all test vessels of the sample are soaked in 3:1 nitric acid for 12 hours before use, and drying in an oven at 65 ℃ for later use.
(4) Measurement of piglet blood biochemical index
Cytochrome P450 oxidoreductase (CPR) enzyme, cytochrome oxidase (CCO), ferritin (SF) and Myoglobin (MB) are measured by adopting a biotin double antibody sandwich enzyme-linked immunosorbent assay (ELISA), and a total plasma iron binding force (TIBC) kit is purchased from Shanghai Xinle company.
(5) Statistical analysis of data
The test data were initially processed using Excel and statistically processed using PASS 18.0 software.
The results of test one are shown in Table 16, and as can be seen from Table 16: the iron content in the feces of the piglets of the test group is not obviously different from that of the control group, but the iron content in the feces has a descending trend (P > 0.05) compared with the control group; compared with the control group, the iron content in the liver of each piglet is obviously improved (P < 0.05), and the difference between the groups I, II and III is obvious (P < 0.05); the difference between the test groups of piglet kidney iron content was not significant (P > 0.05), but there was an upward trend. The hemoglobin test I, II, III showed a significant increase and a significant difference (P < 0.05) from the control group.
Table 16 effect of additives on the prevention of anaemia in piglets
Note that: the same row of lower-case letters have different meanings, which indicates that the difference is significant (P < 0.05);
the results of the second test are shown in Table 17, and from Table 17, it can be seen that: the iron content in the test group piglet manure is obviously different from that in the control group (P is less than 0.05), and the iron content in the test group manure has a lower trend compared with that in the control group; compared with the control group, the iron content in the liver of each piglet in the test group is obviously improved (P < 0.05), and the difference between the IV, V and VI groups in the test is obvious (P < 0.05); the difference between the test groups of piglet kidney iron content in the nursing stage is not obvious (P > 0.05), but the piglet kidney iron content has an ascending trend. The hemoglobin test IV, V and VI groups are obviously increased compared with the control group, and the difference is obvious (P < 0.05).
Effect of additives of table 17 on the prevention of anaemia in nursery pigs
Note that: the same row of lower-case letters have different meanings, which indicates that the difference is significant (P < 0.05);
the results of the test three are shown in Table 18, and from Table 18, it can be seen that: the iron content in the piglet manure of the test group has obvious reduction trend and obvious difference (P is less than 0.05) compared with the control group, so that the test VII group has the best effect; the iron content in the liver of the piglet, the effect of the test VII is optimal, the difference is obvious (P is less than 0.05), and the test VIII, IX groups and the control group have an improvement trend but are not obvious; the difference between the test groups of piglet kidney iron content was not significant (P > 0.05), but there was an upward trend. The hemoglobin test VII, VIII, IX groups had a trend of increasing with the control group, but the difference was not significant (P > 0.05).
Table 18 effect of additives on the prevention of anaemia in piglets
Note that: the same row of lower-case letters have different meanings, which indicates that the difference is significant (P < 0.05);
2.4 influence of additives on economic benefits of piglets
(1) Measurement method
Piglet gain income = average weight gain x piglet live weight unit price;
feed cost = feed unit price x average consumption;
average piglet profit = piglet gain income-feed cost-additive cost.
(2) Effect of additives on economic benefits of piglets
As shown in Table 19, it is clear from Table 19 that the additive can promote the growth of piglets and increase the economic benefit under the same feeding conditions and without the difference in the price of feed. The additive II, III and IV used in the test has the best effect in the test II, the average income is increased by 21.48 yuan per head after 10 days of weaning, the net benefit income is increased by 13.9 percent, and for 1 pig farm with 500 basic sows, the annual benefit income can be increased by more than 20 ten thousand yuan only by using the additive in the piglet stage.
TABLE 19 Effect of different treatments on piglets
The results of the second test are shown in Table 20, and it is clear from Table 20 that the additive can promote the growth of the nursery pigs and improve the economic benefit under the condition of no difference of the same feeding conditions and the feed price. Among the additive IV, V and VI used in the test, the economic benefit of the test IV is the best, and the number of piglets in the conservation stage is increased by 10.6 yuan per piglet during 10 days of the test.
Table 20 effects of different treatments on piglets
The results of the test three are shown in Table 21, and it is clear from Table 21 that the additive can promote the growth of piglets and improve the economic benefit under the condition of no difference between the same feeding conditions and the feed price. The economic benefit of the test VII group is the best in all the control groups, VII, VIII and IX groups, and the income of piglets is increased by 11 yuan each time during 20 days of the test, so that the benefit of income increase is obvious.
TABLE 21 Effect of different treatments on piglets
According to the analysis of the test data, the additive can obviously improve the daily gain of piglets, reduce the feed conversion ratio, effectively prevent the occurrence of diarrhea of the piglets and improve the economic value.
Iron is a trace element necessary for piglets, and influences the growth, development, metabolic process and physiological defense function of piglets. Liver is its main storage site. The absorbed iron is first stored in the liver etc. and released from the storage into the plasma when the body needs it. Iron in plasma is hardly reversely transferred into the intestinal tract. The iron released when various iron-containing substances in the body are degraded is almost completely reused by the body. Most of the iron in the manure is unabsorbed iron in the feed. Small amounts of platelets degraded in the body are excreted from the body as bile and intestinal mucosal cells shed. The average hemoglobin concentration of the piglets is more than 100g/L, so that the iron requirement of the piglets can be met, and the average hemoglobin concentration of the piglets is 80g/L, which indicates that the piglets are close to anemia. The average hemoglobin concentration of piglets in each test group is higher than that in a control group (P < 0.05), and the analysis result of the test data shows that the additive can effectively improve the iron absorption capacity of piglets and effectively prevent the occurrence of piglet anemia.
The total protein comprises albumin, immunoglobulin, etc., and besides the immune function of the immunoglobulin, the albumin also has the functions of maintaining the osmotic pressure of plasma colloid, maintaining the normal PH value of plasma, transporting, catalyzing, nourishing, etc. in the organism. Immunoglobulins are a class of globulins produced in pigs after stimulation with antigens that interact specifically with antigens, also known as antibodies. Immunoglobulins have biological functions of binding antigens, activating complement and modulating action. Wherein IgG, igM, igA is three important classes of immunoglobulins, igG is the immunoglobulin with the highest content in serum, is a main antibody for mediating liquid immunity, plays the immunological activities of antibiosis, antivirus, antitoxin and the like in vivo, and is also indispensable in anti-tumor immunity; igM is the earliest immunoglobulin produced by the primary humoral immune reaction of the animal body, but has short duration, has the immune activities of resisting bacteria, resisting viruses, neutralizing toxins and the like, and also has the anti-tumor effect; igA has the same immunological activities as those of IgA and plays an important role in 'barrier' defense for local mucosal immunity of the respiratory tract, the digestive tract and the like of the organism. Therefore, the increase of piglet stage serum IgA, igM, igG can reduce the occurrence of piglet diseases to some extent. The above test results showed that: the IgA, igM, igG content of each treatment group was increased to a different extent than that of the control group. This demonstrates that the additive used in the above experiments has an effect of increasing the content of piglet serum IgA, igM, igG, and is characterized by reduced diarrhea rate and enhanced disease resistance in production.
Complement is a main component of specific immunity, is a group of proteins with similar enzyme activities, participates in the protective immune response and self-stabilization function of organisms, assists antibodies and phagocytes in killing pathogenic microorganisms and enhancing the cellular immune function, and plays a role in resisting non-specific infection of organisms. The above experiments showed that: the complement C3 and C4 of each group are higher than those of a control group, which indicates that the additive used in the test can improve the content of the complement C3 and C4 in the piglet blood. By blood immune index: the additive has the advantages that the levels of LTR, serum IgG, igM, igA and complement C3 and C4 are obviously improved, namely, the immunity and disease resistance of piglets are improved, wherein each immunity index is better in test I and II groups (IV, V groups and VII, VIII groups), the feeding characteristics and economic benefit analysis of each stage of piglets are integrated, and the additive is optimal in test I groups (IV and VII groups).
Claims (5)
1. The coated nutritional feed additive for preventing anemia and promoting immunity of piglets is characterized by being prepared from, by mass, 19.0-19.3 parts of silicon dioxide, 27.0-27.5 parts of lactic acid, 1.50-2.50 parts of fumaric acid, 0.80-1.20 parts of aminolevulinic acid, 0.80-1.20 parts of citric acid, 0.04-0.05 part of vitamin B group, 0.40-0.60 part of phosphorylated vitamin C, 4.50-5.50 parts of fish meal, 1.50-2.50 parts of glycine chelated iron, 6.50-7.50 parts of L-arginine-alpha-ketoglutarate, 9.0-11.0 parts of oligosaccharide, 90-110 parts of pyruvic acid and 800-850 parts of rice bran meal;
the preparation method of the coated nutritional feed additive comprises the following steps:
step 1: mixing silicon dioxide with lactic acid, adding pulverized and sieved fumaric acid, aminolevulinic acid and citric acid, continuously mixing, adding povidone solution, sequentially extruding, rounding, granulating, drying and sieving to obtain pellets, and coating the pellets in a boiling fluidized bed to obtain coated particles;
wherein the size of the pellets is 35-45 meshes; the coating is carried out by adopting an ethanol solution of polyacrylic resin, wherein the polyacrylic resin is one or two of polyacrylic resin II and polyacrylic resin IV, and the mass concentration of the polyacrylic resin in the ethanol solution of the polyacrylic resin is 2% -7%;
step 2: after crushing rice bran meal, firstly putting a part of crushed rice bran meal into a mixer, then adding vitamin B group, phosphorylated vitamin C and fish meal after preliminary screening for mixing, after mixing uniformly, adding another part of rice bran meal, glycine chelated iron, L-arginine alpha-ketoglutarate and oligosaccharide for continuous mixing, after mixing uniformly, adding the rest rice bran meal, pyruvic acid creatine and the coated particles obtained in the step 1, and continuously mixing until uniformity, thus obtaining the coated nutritional feed additive for preventing piglet anemia and promoting immune function.
2. The coated nutritional feed additive for preventing anemia and promoting immune function in piglets according to claim 1, wherein the vitamin B group is one or a mixture of several of vitamin B6, vitamin B1 and vitamin B12.
3. The coated nutritional feed additive for preventing anemia and promoting immune function of piglets according to claim 1, wherein the oligosaccharide is one or a mixture of several of chitosan oligosaccharide, fructooligosaccharide, mannooligosaccharide and xylooligosaccharide.
4. The coated nutritional feed additive for preventing anemia and promoting immune function in piglets according to claim 1, wherein the ratio of the sum of the mass of silica, lactic acid, fumaric acid, aminolevulinic acid, citric acid to the volume of povidone solution in step 1 is 7g:3mL.
5. The coated nutritional feed additive for preventing anemia and promoting immunity of piglets according to claim 1, wherein in the step 2, a part of crushed rice bran meal is 30% -50% of the total mass of the rice bran meal, in the step 2, another part of rice bran meal is 30% -50% of the total mass of the rice bran meal, in the step 2, the primary screening is screening with a 40-mesh sieve, in the step 2, mixing is carried out by adopting a three-dimensional mixer, in the step 2, vitamin B group, phosphorylated vitamin C and the primary screened fish meal are added, mixing is carried out for 15-20 min, another part of rice bran meal, glycine chelated iron, L-arginine alpha-ketoglutarate and oligosaccharide are added, mixing is continued for 15-20 min, and the rest rice bran meal, pyruvic acid and the coated particles obtained in the step 1 are added, and mixing is continued for 15-20 min.
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| CN1404746A (en) * | 2002-10-31 | 2003-03-26 | 俞祖勋 | Thin-meat type feed additive |
| CN102028102A (en) * | 2010-12-08 | 2011-04-27 | 江南大学 | Natural and safe growth-type feed additive |
| CN102578387A (en) * | 2012-02-03 | 2012-07-18 | 北京大北农科技集团股份有限公司 | Slowly-released compound acidifier for poultry and livestock feed, preparation method thereof and feed |
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| CN113170840A (en) * | 2021-05-20 | 2021-07-27 | 河南邑鸿善成生物技术有限公司 | Feed additive containing 5-aminolevulinic acid and ferrous glycinate as well as preparation method and application thereof |
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