CN114868738A - Stem cell storage system with temperature monitoring device - Google Patents
Stem cell storage system with temperature monitoring device Download PDFInfo
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- CN114868738A CN114868738A CN202210721567.2A CN202210721567A CN114868738A CN 114868738 A CN114868738 A CN 114868738A CN 202210721567 A CN202210721567 A CN 202210721567A CN 114868738 A CN114868738 A CN 114868738A
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- 210000000130 stem cell Anatomy 0.000 title claims abstract description 30
- 238000012806 monitoring device Methods 0.000 title claims abstract description 14
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 65
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 31
- 230000007246 mechanism Effects 0.000 claims abstract description 29
- 239000007788 liquid Substances 0.000 claims abstract description 27
- 238000004321 preservation Methods 0.000 claims description 26
- 238000007789 sealing Methods 0.000 claims description 14
- 238000005086 pumping Methods 0.000 claims description 9
- 238000003825 pressing Methods 0.000 claims description 7
- 238000009413 insulation Methods 0.000 claims 2
- 238000012544 monitoring process Methods 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 5
- 230000009471 action Effects 0.000 description 7
- 238000003780 insertion Methods 0.000 description 6
- 230000037431 insertion Effects 0.000 description 6
- 238000009434 installation Methods 0.000 description 6
- 239000007787 solid Substances 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 4
- 238000000605 extraction Methods 0.000 description 4
- 238000010030 laminating Methods 0.000 description 4
- 208000027418 Wounds and injury Diseases 0.000 description 3
- 229910001873 dinitrogen Inorganic materials 0.000 description 3
- 230000006698 induction Effects 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 230000002441 reversible effect Effects 0.000 description 3
- 230000006378 damage Effects 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 230000002035 prolonged effect Effects 0.000 description 2
- 239000000565 sealant Substances 0.000 description 2
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- WABPQHHGFIMREM-UHFFFAOYSA-N lead(0) Chemical compound [Pb] WABPQHHGFIMREM-UHFFFAOYSA-N 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 238000004804 winding Methods 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
- A01N1/0252—Temperature controlling refrigerating apparatus, i.e. devices used to actively control the temperature of a designated internal volume, e.g. refrigerators, freeze-drying apparatus or liquid nitrogen baths
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- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- Mechanical Engineering (AREA)
- Health & Medical Sciences (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
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Abstract
The invention discloses a stem cell storage system with a temperature monitoring device, which comprises a heat-insulating tank and a containing tank, wherein the containing tank is positioned inside the heat-insulating tank, a fixing ring is fixedly connected on the heat-insulating tank, a temperature sensor for controlling an electromagnetic valve is fixedly connected in the containing tank, and an air exhaust mechanism for maintaining the vacuum degree of a gap between the heat-insulating tank and the containing tank is arranged on a cover plate. The gap between the containing tank and the heat-insulating tank is vacuumized while the liquid nitrogen is added by arranging the air exhaust mechanism, so that the heat is not easy to transfer into the containing tank, make the apron to hug closely through setting up the loading system and hold the jar, avoid making after the time has long and hold the sealed effect variation of jar.
Description
Technical Field
The invention relates to the technical field of medical stem cells, in particular to a stem cell storage system with a temperature monitoring device.
Background
The stem cell is a kind of multipotential cell with self-replicating ability, under a certain condition, it can be differentiated into various functional cells, and the stem cell has the potential function of regenerating various tissues and organs and human body, and is called universal cell in medical field.
At present, stem cells need a storage device with certain conditions for storage, but in the actual use of the stem cell storage device, under the condition that a storage tank is stored for a long time, liquid nitrogen can volatilize, the temperature is increased, and the stem cell storage device is not suitable for stem cell storage.
Disclosure of Invention
The stem cell storage system with the temperature monitoring device has the advantage of automatically supplementing liquid nitrogen, and solves the problem that stem cells are invalid due to volatilization of liquid nitrogen.
In order to achieve the purpose, the invention provides the following technical scheme: the utility model provides a stem cell storage system with temperature monitoring device, includes the heat preservation jar and holds the jar, it is located to hold the jar the inside of heat preservation jar, the solid fixed ring of fixedly connected with on the heat preservation jar, gu fixed ring fixed connection is in the inner wall of heat preservation jar, but install detachable design's the shutoff that is used for on the heat preservation jar hold the apron of jar, fixedly connected with liquid nitrogen induction pipe on the apron, the liquid nitrogen induction pipe runs through the apron extends to hold the inside of jar, lie in on the liquid nitrogen induction pipe the upper portion of apron is equipped with the solenoid valve, hold jar internal fixedly connected with and be used for control the temperature sensor of solenoid valve, be equipped with on the apron and be used for maintaining the heat preservation jar with hold the air exhaust mechanism of clearance vacuum between the jar.
Preferably, the air exhaust mechanism includes the blast pipe, blast pipe fixed connection be in on the apron, just the blast pipe with it is linked together to hold the jar, lie in on the blast pipe the upper portion of apron is equipped with first check valve, lie in the blast pipe the upper portion fixedly connected with of first check valve keeps off the ring, sliding connection has the plectane in the blast pipe, the plectane with the inner wall laminating of blast pipe, keep off the ring with the plectane offsets, fixedly connected with rack on the plectane, seted up on the blast pipe with rack complex hole, the spring has been cup jointed on the rack, the both ends of spring respectively with the plectane with the blast pipe offsets.
Preferably, the air exhaust mechanism further comprises a support fixedly connected to the exhaust pipe, the support is rotatably connected with a gear meshed with the rack, the gear is provided with a disc coaxially and fixedly connected with, the disc is provided with an eccentric rod, the eccentric rod is slidably connected with a hollow bar, the hollow bar is provided with an installation rod fixedly connected with, and the installation rod is provided with a first piston plate fixedly connected with one end of the hollow bar.
Preferably, the air exhaust mechanism further comprises an air exhaust pipe, the air exhaust pipe is fixedly connected to the fixing ring, the bottom of the air exhaust pipe penetrates through the fixing ring and extends to the heat preservation tank and the gap between the holding tanks, a sealing ring is fixedly connected to the top of the air exhaust pipe, a through hole matched with the air exhaust pipe is formed in the cover plate, a vertical pipe is fixedly connected to the cover plate and sleeved on the air exhaust pipe, a second one-way valve and a third one-way valve are arranged on the vertical pipe, a transverse pipe is fixedly connected between the second one-way valve and the third one-way valve and is communicated with the vertical pipe, the first piston plate is slidably connected to the transverse pipe, and the first piston plate is attached to the inner wall of the transverse pipe.
Preferably, the air exhaust mechanism further comprises a spiral pipe wound on the horizontal pipe, the spiral pipe is fixedly connected to the exhaust pipe, and the joint of the spiral pipe and the exhaust pipe is located on the upper portion of the circular plate.
Preferably, the air exhaust mechanism is provided with a pressurizing mechanism which is used for ensuring the cover plate to be tightly attached to the containing tank for the pressure of the cover plate so as to ensure the sealing property of the containing tank, the pressurizing mechanism comprises a rectangular plate fixedly connected to the heat-preserving tank, the rectangular plate is connected with an installation frame in a sliding way, a clamping block matched with the rectangular plate is fixedly connected on the mounting frame, a piston cylinder is fixedly connected on the mounting frame, the top of the piston cylinder is fixedly connected with a pressure relief pipe, the pressure relief pipe is provided with a pressure valve, a second piston plate is connected in the piston cylinder in a sliding way, the bottom of the second piston plate is fixedly connected with a pressure lever, the bottom of the pressure lever is fixedly connected with a pressure plate, the pressing plate is abutted to the cover plate, the piston cylinder is fixedly connected with an air guide pipe, and one end of the air guide pipe, which is far away from the piston cylinder, is fixedly connected to the vertical pipe.
Preferably, fixedly connected with stopper on the apron, it is connected with the shifting block to rotate on the heat preservation jar, the symmetry rotates on the shifting block and is connected with two L shape poles, two equal fixedly connected with drum on the L shape pole, be equipped with n shape pole on the drum, the both ends of n shape pole correspond sliding connection respectively two in the drum, two equal sliding connection has the inserted bar on the drum, set up on the n shape pole a plurality of with inserted bar complex slot, keep away from on the inserted bar the one end fixedly connected with spacing ring of n shape pole, cup jointed first extension spring on the inserted bar, the both ends of first extension spring respectively fixed connection in the drum with on the spacing ring, be equipped with the second extension spring in the drum, the both ends of second extension spring respectively fixed connection in n shape pole with on the drum.
Compared with the prior art, the invention has the following beneficial effects:
1. according to the invention, the temperature sensor is arranged to monitor the temperature in the containing tank in real time, the liquid nitrogen is automatically added, the normal storage of stem cells is ensured, the air exhaust mechanism is arranged to vacuumize the gap between the containing tank and the heat preservation tank while the liquid nitrogen is added, the heat is ensured not to be easily transferred into the containing tank, the cover plate can be tightly attached to the containing tank by arranging the pressurizing mechanism, and the problem that the sealing effect of the containing tank is poor after a long time is avoided.
Drawings
FIG. 1 is a schematic external view of the present invention;
FIG. 2 is a schematic view of the internal structure of the present invention;
FIG. 3 is a schematic structural view of portion A of FIG. 2 according to the present invention;
FIG. 4 is a schematic view of the present invention at a gear;
FIG. 5 is a schematic view of the pressing mechanism according to the present invention;
FIG. 6 is a schematic view of the structure at the cylinder of the present invention;
fig. 7 is a schematic structural diagram of part B in fig. 6 according to the present invention.
In the figure: 1. a heat preservation tank; 11. a fixing ring; 12. an air exhaust pipe; 13. a seal ring; 14. vacuumizing a tube; 15. a valve; 2. a cover plate; 21. a liquid nitrogen inlet pipe; 22. an electromagnetic valve; 23. an exhaust pipe; 24. a first check valve; 25. a rack; 26. a circular plate; 27. a baffle ring; 28. a spring; 29. a support; 3. a mounting frame; 31. a piston cylinder; 32. a pressure relief pipe; 33. a pressure valve; 34. a pressure lever; 35. a second piston plate; 36. pressing a plate; 37. a clamping block; 38. a rectangular plate; 4. a holding tank; 41. a temperature sensor; 5. a vertical tube; 51. a second one-way valve; 52. a third check valve; 53. a transverse tube; 54. a gear; 55. an eccentric rod; 56. a hollow bar; 57. mounting a rod; 58. a first piston plate; 59. an air duct; 6. a spiral tube; 61. a disc; 7. shifting blocks; 71. an L-shaped rod; 72. a cylinder; 73. an n-shaped rod; 74. a limiting block; 75. inserting a rod; 76. a first tension spring; 77. a limiting ring; 78. the second extension spring.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example one
The invention provides a technical scheme that: the utility model provides a stem cell storage system with temperature monitoring device, include heat preservation tank 1 and hold jar 4, hold jar 4 and be located heat preservation tank 1's inside, the solid fixed ring 11 of fixedly connected with on the heat preservation tank 1, solid fixed ring 11 fixed connection is at heat preservation tank 1's inner wall, the apron 2 that is used for the shutoff to hold jar 4 of installing detachable design on the heat preservation tank 1, fixedly connected with liquid nitrogen inlet-tube 21 on the apron 2, liquid nitrogen inlet-tube 21 runs through apron 2 and extends to the inside that holds jar 4, the upper portion that lies in apron 2 on the liquid nitrogen inlet-tube 21 is equipped with solenoid valve 22, hold jar 4 internal fixation connected with and be used for controlling solenoid valve 22's temperature sensor 41, be equipped with on the apron 2 and be used for maintaining heat preservation tank 1 and hold the mechanism of bleeding of the clearance vacuum between the jar 4.
Referring to fig. 1 and 2, stem cells are placed in a holding tank 4, a vertical pipe 5 on a cover plate 2 is aligned with an air extraction pipe 12, the cover plate 2 is covered on the holding tank 4 and a heat preservation tank 1, the air extraction pipe 12 is inserted into the vertical pipe 5, a sealing ring 13 on the air extraction pipe 12 is attached to the inner wall of the vertical pipe 5, the sealing performance of the connection between the vertical pipe 5 and the air extraction pipe 12 is guaranteed, at the moment, a dial block 7 is moved upwards to enable an n-shaped rod 73 to be hung on a limiting block 74, then, an installation frame 3 is clamped on a rectangular plate 38 through a clamping block 37, the installation frame 3 is pushed to enable a piston cylinder 31 on the installation frame 3 to be located above the cover plate 2, the cover plate 2 is covered on the holding tank 4, then, a temperature sensor 41 is started to work, a lead out of a lead wire on the temperature sensor 41 through a perforation on the cover plate 2 under the premise that the holding tank 4 is sealed, then, sealant is coated at the perforation, the temperature of the temperature sensor 41 is set, there is not the liquid nitrogen in holding jar 4 when depositing stem cell for the first time, and temperature sensor 41 control solenoid valve 22 opens this moment, and then high-pressure nitrogen gas enters into through liquid nitrogen inlet tube 21 and holds jar 4 in for the temperature that holds in jar 4 reduces, and this process takes place after holding jar 4 and seal, and then can avoid the liquid nitrogen to leak and cause the injury to personnel.
Air exhaust mechanism includes blast pipe 23, blast pipe 23 fixed connection is on apron 2, and blast pipe 23 is linked together with holding jar 4, the upper portion that lies in apron 2 on blast pipe 23 is equipped with first check valve 24, the upper portion fixedly connected with who lies in first check valve 24 in blast pipe 23 keeps off the ring 27, sliding connection has plectane 26 in blast pipe 23, plectane 26 and the inner wall laminating of blast pipe 23, keep off ring 27 and plectane 26 and offset, fixedly connected with rack 25 on the plectane 26, seted up on blast pipe 23 with rack 25 complex hole, the spring 28 has been cup jointed on the rack 25, the both ends of spring 28 offset with plectane 26 and blast pipe 23 respectively.
Referring to fig. 2, 3 and 4, the space inside the holding tank 4 is in a sealed state after the cover plate 2 is installed, so that the pressure inside the holding tank 4 is increased when liquid nitrogen is introduced into the holding tank 4, and the high pressure in the holding tank 4 makes the circular plate 26 in the exhaust pipe 23 move upward, and further makes the rack 25 fixedly connected to the circular plate 26 move upward in synchronization with the circular plate 26, so that the gear 54 engaged with the rack 25 is rotated, and thus the disc 61 fixedly coupled to the gear 54 is rotated, and the eccentric rod 55 of the disc 61 is slid in the hollow bar 56, because the vertical pipe 5 is fixedly connected on the cover plate 2 and the horizontal pipe 53 is fixedly connected on the vertical pipe 5, the relative position of the horizontal pipe 53 and the cover plate 2 can not be changed, and the first piston plate 58 slides in the cross tube 53, the hollow bar 56 is fixedly connected with the first piston plate 58 through the mounting rod 57, and in turn, the first piston plate 58 can be caused to slide within the cross tube 53 as the eccentric rod 55 slides within the hollow bar 56.
The air exhaust mechanism further comprises a support 29 fixedly connected to the exhaust pipe 23, a gear 54 meshed with the rack 25 is rotatably connected to the support 29, a disc 61 is coaxially and fixedly connected to the gear 54, an eccentric rod 55 is fixedly connected to the disc 61, a hollow bar 56 is slidably connected to the eccentric rod 55, a mounting rod 57 is fixedly connected to the hollow bar 56, and a first piston plate 58 is fixedly connected to one end, far away from the hollow bar 56, of the mounting rod 57.
Referring to fig. 2, 3 and 4, when the circular plate 26 slides to the connection position of the spiral pipe 6 and the exhaust pipe 23, the high-pressure air in the tank 4 is exhausted from the spiral pipe 6, so that the thrust of the circular plate 26 by the exhausted air in the tank 4 is instantaneously reduced, the circular plate 26 moves downward under the action of the spring 28, the rack 25 fixedly connected to the circular plate 26 moves downward in synchronization with the circular plate 26, the gear 54 engaged with the rack 25 rotates in reverse, the circular plate 61 rotates in reverse, the first piston plate 58 slides in reverse, and the circular plate 26 moves upward under the thrust of the exhausted air in the tank 4, reciprocates, and the first piston plate 58 slides in a reciprocating manner in the horizontal pipe 53.
Air exhaust mechanism still includes exhaust tube 12, exhaust tube 12 fixed connection is on solid fixed ring 11, the bottom of exhaust tube 12 runs through solid fixed ring 11 and extends to heat preservation jar 1 and hold in the clearance between jar 4, the top fixedly connected with sealing washer 13 of exhaust tube 12, set up on the apron 2 with exhaust tube 12 complex through-hole, fixedly connected with standpipe 5 on the apron 2, standpipe 5 cup joints on exhaust tube 12, be equipped with second check valve 51 and third check valve 52 on the standpipe 5, lie in the horizontal pipe 53 of fixedly connected with between second check valve 51 and the third check valve 52 on the standpipe 5, horizontal pipe 53 is linked together with standpipe 5, first piston plate 58 sliding connection is in horizontal pipe 53, and the inner wall laminating of first piston plate 58 and horizontal pipe 53.
Referring to fig. 2, 3 and 4, since the horizontal tube 53 is communicated with the vertical tube 5, and thus air in the gap between the insulated tank 1 and the holding tank 4 is drawn out through the suction pipe 12 and the vertical tube 5 when the first piston plate 58 is reciprocally slid in the horizontal tube 53, the second check valve 51 and the third check valve 52 are provided such that the drawn air does not flow backward.
The air exhaust mechanism also comprises a spiral pipe 6 wound on the transverse pipe 53, the spiral pipe 6 is fixedly connected on the exhaust pipe 23, and the joint of the spiral pipe 6 and the exhaust pipe 23 is positioned at the upper part of the circular plate 26.
Referring to fig. 2 and 3, volatile low temperature nitrogen gas then can discharge through spiral pipe 6 when letting in the liquid nitrogen to holding jar 4, and spiral pipe 6 winding is violently managed 53, and then low temperature nitrogen gas makes violently managing 53 to receive the cold shrink for it is more intimate to violently manage the laminating between 53 and the first piston plate 58, strengthens the effect to holding jar 1 and hold the evacuation of space between jar 4.
Example two
On the basis of embodiment one, further, be equipped with on the air exhaust mechanism and be used for making 2 pressure of apron to hug closely the loading mechanism who holds jar 4 in order to guarantee to hold jar 4 leakproofness for 2 pressure of apron, loading mechanism includes rectangular plate 38 of fixed connection on heat preservation jar 1, sliding connection has mounting bracket 3 on the rectangular plate 38, fixedly connected with and rectangular plate 38 complex fixture block 37 on the mounting bracket 3, fixedly connected with piston cylinder 31 on the mounting bracket 3, the top fixedly connected with pressure release pipe 32 of piston cylinder 31, be equipped with pressure valve 33 on the pressure release pipe 32, sliding connection has second piston plate 35 in the piston cylinder 31, the bottom fixedly connected with depression bar 34 of second piston plate 35, the bottom fixedly connected with clamp plate 36 of depression bar 34, clamp plate 36 offsets with apron 2, fixedly connected with air duct 59 on the piston cylinder 31, the one end fixed connection who keeps away from piston cylinder 31 on the air duct 59 is on standpipe 5.
Referring to fig. 1 and 5, the air extracted through the standpipe 5 enters the piston cylinder 31 through the air duct 59, and then the pressure in the piston cylinder 31 increases, and then the second piston plate 35 in the piston cylinder 31 slides downwards, and then the second piston plate 35 drives the pressure lever 34 and the pressure plate 36 to move downwards, and then the pressure plate 36 moving downwards extrudes the cover plate 2, increase the cover plate 2 and hold the degree of closeness of jar 4, because the pressure valve 33 is arranged on the pressure relief pipe 32, and then when the pressure in the second piston cylinder 31 is too large, the inside high-pressure air of the second piston cylinder 31 can be discharged through the pressure relief pipe 32.
EXAMPLE III
On the basis of the first embodiment, further, a limit block 74 is fixedly connected to the cover plate 2, a shifting block 7 is rotatably connected to the heat preservation tank 1, two L-shaped rods 71 are symmetrically and rotatably connected to the shifting block 7, cylinders 72 are fixedly connected to the two L-shaped rods 71, n-shaped rods 73 are arranged on the cylinders 72, two ends of each n-shaped rod 73 are respectively connected to the two cylinders 72 in a corresponding sliding mode, inserting rods 75 are respectively and slidably connected to the two cylinders 72, a plurality of inserting grooves matched with the inserting rods 75 are formed in the n-shaped rods 73, a limit ring 77 is fixedly connected to one end, far away from the n-shaped rods 73, of each inserting rod 75, a first tension spring 76 is sleeved on each inserting rod 75, two ends of the first tension spring 76 are respectively and fixedly connected to the cylinders 72 and the limit ring 77, a second tension spring 78 is arranged in the cylinders 72, and two ends of the second tension spring 78 are respectively and fixedly connected to the n-shaped rods 73 and the cylinders 72.
Referring to fig. 1, 6 and 7, when the cover 2 is pressed by the pressing plate 36, the cover 2 is pressed more tightly against the can 4 (the cover 2 is designed as a conventional sealing cover with a sealing cushion, not shown), the pressing of the pressing plate 36 presses the sealing cushion on the cover 2 more flat, and the distance between the cover plate 2 and the holding tank 4 is closer, and the limit block 74 on the cover plate 2 is closer to the heat preservation tank 1, at this time, the n-shaped rod 73 slides towards the inside of the cylinder 72 under the action of the second tension spring 78, as shown in fig. 7, the end of the inserted rod 75 connected with the n-shaped rod 73 is provided with an inclination angle, when the n-shaped rod 73 moves downwards, the inserted rod 75 is pushed away from the n-shaped rod 73 along the inclination angle of the inserted rod 75, and as the n-shaped rod 73 continues to move downwards, the inserted rod 75 is inserted into the next insertion hole under the action of the first tension spring 76, so that the cover plate 2 is further locked.
Fixedly connected with is used for carrying out the evacuation tube 14 of evacuation for holding the clearance between jar 4 and the holding jar 1 on the holding jar 1, is equipped with valve 15 on the evacuation tube 14.
Referring to fig. 1 and 2, a vacuum pump is connected to the evacuation tube 14 and the valve 15 is opened to further evacuate the gap between the holding tank 4 and the heat-preserving tank 1, so that the external heat cannot be easily conducted into the holding tank 4, and the storage time of stem cells is prolonged.
The working principle is as follows: this stem cell storage system with temperature monitoring device, place the stem cell in holding jar 4 during the use, 2 lids jar 4 and heat preservation jar 1 with 2 lids of apron with 5 alignment exhaust tube 12 of standpipe on the apron again, make exhaust tube 12 insert in the standpipe 5, sealing washer 13 on the exhaust tube 12 pastes with 5 inner walls of standpipe, guarantee the leakproofness that standpipe 5 and exhaust tube 12 are connected, stir shifting block 7 upwards again this moment and make n shape pole 73 hang on stopper 74, pass through 37 cards of fixture block on rectangular plate 38 with mounting bracket 3 afterwards, promote mounting bracket 3 again and make the piston cylinder 31 on mounting bracket 3 be located on apron 2.
After the cover plate 2 is covered on the containing tank 4, the temperature sensor 41 is started to work, a lead on the temperature sensor 41 can be led out through a through hole on the cover plate 2 on the premise that the containing tank 4 is guaranteed to be closed, then sealant is coated on the through hole, the working temperature is set for the temperature sensor 41, when stem cells are stored for the first time, liquid nitrogen does not exist in the containing tank 4, the electromagnetic valve 22 is controlled to be opened by matching with the temperature sensor 41, then high-pressure nitrogen enters the containing tank 4 through the liquid nitrogen inlet pipe 21, the temperature in the containing tank 4 is reduced, the process is carried out after the containing tank 4 is closed, and further, the damage to personnel caused by liquid nitrogen leakage can be avoided;
after the cover plate 2 is installed, the space in the holding tank 4 is in a closed state, when liquid nitrogen is introduced into the holding tank 4, the pressure in the holding tank 4 is increased, and then the high pressure in the holding tank 4 causes the circular plate 26 in the exhaust pipe 23 to move upwards, the arrangement of the first check valve 24 causes the gas in the holding tank 4 to only pass out from the exhaust pipe 23, and the external gas cannot enter the holding tank 4 from the exhaust pipe 23, and further causes the rack 25 fixedly connected to the circular plate 26 and the circular plate 26 to move upwards synchronously, so that the gear 54 engaged with the rack 25 rotates, and the disc 61 fixedly connected to the gear 54 rotates, and further the eccentric rod 55 on the disc 61 slides in the hollow bar 56, because the vertical pipe 5 is fixedly connected to the cover plate 2, the horizontal pipe 53 is fixedly connected to the vertical pipe 5, and further the relative position of the horizontal pipe 53 and the cover plate 2 is not changed, and the first piston plate 58 slides in the horizontal pipe 53, the hollow bar 56 is fixedly connected with the first piston plate 58 through the mounting rod 57, and further the first piston plate 58 can be driven to slide in the transverse pipe 53 when the eccentric rod 55 slides in the hollow bar 56;
when the circular plate 26 slides to the joint of the spiral pipe 6 and the exhaust pipe 23, the high-pressure air in the tank 4 is exhausted from the spiral pipe 6, so that the thrust of the circular plate 26 on the exhaust air in the tank 4 is instantaneously reduced, the circular plate 26 moves downwards under the action of the spring 28, the rack 25 fixedly connected to the circular plate 26 and the circular plate 26 synchronously move downwards, the gear 54 meshed with the rack 25 rotates reversely, the circular plate 61 rotates reversely, the first piston plate 58 slides reversely, and the circular plate 26 moves upwards under the action of the thrust of the exhaust air in the tank 4 to reciprocate, so that the first piston plate 58 slides in a reciprocating manner in the transverse pipe 53;
because the transverse pipe 53 is communicated with the vertical pipe 5, when the first piston plate 58 slides in the transverse pipe 53 in a reciprocating manner, air in a gap between the heat-insulating tank 1 and the containing tank 4 is pumped out through the air pumping pipe 12 and the vertical pipe 5, the air pumped out in the gap between the heat-insulating tank 1 and the containing tank 4 is led out through the air guide pipe 59, and the arrangement of the second check valve 51 and the third check valve 52 can prevent the pumped air from flowing back;
the air pumped through the standpipe 5 enters the piston cylinder 31 through the air duct 59, so that the pressure in the piston cylinder 31 is increased, the second piston plate 35 in the piston cylinder 31 slides downwards, the second piston plate 35 drives the pressure rod 34 and the pressure plate 36 to move downwards, the pressure plate 36 moving downwards extrudes the cover plate 2, the tightness between the cover plate 2 and the tank 4 is increased, when the cover plate 2 is extruded by the pressure plate 36, the cover plate 2 is more tightly attached to the tank 4, (the cover plate 2 is designed to be a conventional sealing cover with a sealing cushion not shown), the sealing cushion on the cover plate 2 is pressed to be flatter by the extrusion of the pressure plate 36, the distance between the cover plate 2 and the tank 4 is closer, the limit block 74 on the cover plate 2 is closer to the heat preservation tank 1, at this time, the n-shaped rod 73 slides towards the inside of the cylinder 72 under the action of the second tension spring 78, as shown in fig. 7, an inclination angle is provided at one end of the insertion rod 75 connected with the n-shaped rod 73, and then the insertion rod 75 is pushed to a direction away from the n-shaped rod 73 along the inclination angle on the insertion rod 75 when the n-shaped rod 73 moves downwards, the insertion rod 75 is inserted into the next insertion hole under the action of the first tension spring 76 as the n-shaped rod 73 continues to move downwards, so that the cover plate 2 is further locked, then the vacuum pump is connected to the vacuum-pumping tube 14 and the valve 15 is opened to further vacuum the gap between the holding tank 4 and the heat-preserving tank 1, so that the external heat cannot be easily conducted into the holding tank 4, and the storage time of the dry cells is prolonged;
since the pressure relief pipe 32 is provided with the pressure valve 33, when the pressure inside the second piston cylinder 31 becomes excessive, the high-pressure air inside the second piston cylinder 31 is discharged through the pressure relief pipe 32.
The stem cells can be stored after the operation is completed, the temperature in the holding tank 4 may be changed after long-term storage, the vacuum degree of the gap between the holding tank 4 and the heat-insulating tank 1 also changes, further, in the long-term storage process, when the temperature sensor 41 detects that the temperature in the holding tank 4 is higher than the set temperature, the electromagnetic valve 22 is controlled again to be opened to supplement liquid nitrogen to the holding tank 4, the gap between the holding tank 4 and the heat-insulating tank 1 is vacuumized while the nitrogen is supplemented to the holding tank 4, the vacuum degree of the gap between the heat-insulating tank 1 and the holding tank 4 is maintained, the heat-insulating effect is enhanced, the volatile low-temperature nitrogen while introducing the liquid nitrogen to the holding tank 4 can be discharged through the spiral pipe 6, the spiral pipe 6 is wound on the transverse pipe 53, and then the transverse pipe 53 is cooled and contracted by the low-temperature nitrogen, so that the transverse pipe 53 is more closely attached to the first piston plate 58, strengthen the effect of evacuation to the heat preservation jar 1 and hold the space between the jar 4.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. The utility model provides a stem cell storage system with temperature monitoring device, includes heat preservation jar (1) and holds jar (4), its characterized in that: the containing tank (4) is positioned inside the heat-insulating tank (1), the heat-insulating tank (1) is fixedly connected with a fixing ring (11), the fixing ring (11) is fixedly connected with the inner wall of the heat-insulating tank (1), the heat-insulating tank (1) is provided with a cover plate (2) which is detachably designed and used for plugging the containing tank (4), a liquid nitrogen lead-in pipe (21) is fixedly connected to the cover plate (2), the liquid nitrogen lead-in pipe (21) penetrates through the cover plate (2) and extends to the interior of the containing tank (4), an electromagnetic valve (22) is arranged on the upper part of the cover plate (2) on the liquid nitrogen leading-in pipe (21), a temperature sensor (41) for controlling the electromagnetic valve (22) is fixedly connected in the holding tank (4), and the cover plate (2) is provided with an air exhaust mechanism for maintaining the gap vacuum degree between the heat-insulating tank (1) and the containing tank (4).
2. The stem cell storage system with temperature monitoring device of claim 1, wherein: the air exhaust mechanism comprises an exhaust pipe (23), the exhaust pipe (23) is fixedly connected to the cover plate (2), and the exhaust pipe (23) is communicated with the containing tank (4).
3. The stem cell storage system with temperature monitoring device of claim 2, wherein: the exhaust pipe (23) is located, a first one-way valve (24) is arranged on the upper portion of the cover plate (2), and a retaining ring (27) is fixedly connected to the upper portion of the first one-way valve (24) in the exhaust pipe (23).
4. A stem cell storage system with temperature monitoring apparatus as claimed in claim 3, wherein: the exhaust pipe is characterized in that a circular plate (26) is connected to the exhaust pipe (23) in a sliding manner, the circular plate (26) is attached to the inner wall of the exhaust pipe (23), a baffle ring (27) is abutted to the circular plate (26), a rack (25) is fixedly connected to the circular plate (26), a hole matched with the rack (25) is formed in the exhaust pipe (23), a spring (28) is sleeved on the rack (25), two ends of the spring (28) are respectively abutted to the circular plate (26) and the exhaust pipe (23), the air suction mechanism further comprises a support (29) fixedly connected to the exhaust pipe (23), a gear (54) meshed with the rack (25) is rotatably connected to the support (29), a circular plate (61) is coaxially and fixedly connected to the gear (54), an eccentric rod (55) is fixedly connected to the circular plate (61), and a hollow bar (56) is rotatably connected to the eccentric rod (55), the vacuum pump is characterized in that a mounting rod (57) is fixedly connected to the hollow strip (56), one end, far away from the hollow strip (56), of the mounting rod (57) is fixedly connected with a first piston plate (58), the air pumping mechanism further comprises an air pumping pipe (12), the air pumping pipe (12) is fixedly connected to the fixing ring (11), the bottom of the air pumping pipe (12) penetrates through the fixing ring (11) and extends into a gap between the heat preservation tank (1) and the holding tank (4), a sealing ring (13) is fixedly connected to the top of the air pumping pipe (12), a through hole matched with the air pumping pipe (12) is formed in the cover plate (2), a vertical pipe (5) is fixedly connected to the cover plate (2), the vertical pipe (5) is sleeved on the air pumping pipe (12), a second one-way valve (51) and a third one-way valve (52) are arranged on the vertical pipe (5), a transverse pipe (53) is fixedly connected between the second check valve (51) and the third check valve (52) on the vertical pipe (5), the transverse pipe (53) is communicated with the vertical pipe (5), the first piston plate (58) is connected in the transverse pipe (53) in a sliding mode, and the first piston plate (58) is attached to the inner wall of the transverse pipe (53).
5. The stem cell storage system with temperature monitoring device of claim 4, wherein: the air exhaust mechanism further comprises a spiral pipe (6) wound on the transverse pipe (53), the spiral pipe (6) is fixedly connected to the exhaust pipe (23), and the connection position of the spiral pipe (6) and the exhaust pipe (23) is located on the upper portion of the circular plate (26).
6. The stem cell storage system with temperature monitoring device of claim 5, wherein: the air exhaust mechanism is provided with a pressurizing mechanism used for enabling the cover plate (2) to be tightly attached to the tank (4) to ensure that the tank (4) is hermetically arranged through the pressure of the cover plate (2), the pressurizing mechanism comprises a rectangular plate (38) fixedly connected to the heat-insulating tank (1), a mounting rack (3) is slidably connected to the rectangular plate (38), a clamping block (37) matched with the rectangular plate (38) is fixedly connected to the mounting rack (3), a piston cylinder (31) is fixedly connected to the mounting rack (3), a pressure relief pipe (32) is fixedly connected to the top of the piston cylinder (31), a pressure valve (33) is arranged on the pressure relief pipe (32), a second piston plate (35) is slidably connected to the piston cylinder (31), a pressure rod (34) is fixedly connected to the bottom of the second piston plate (35), and a pressure plate (36) is fixedly connected to the bottom of the pressure rod (34), the pressing plate (36) is abutted to the cover plate (2), the piston cylinder (31) is fixedly connected with an air duct (59), and one end, far away from the piston cylinder (31), of the air duct (59) is fixedly connected to the vertical pipe (5).
7. The stem cell storage system with temperature monitoring device of claim 1, wherein: the heat-insulation tank is characterized in that a limiting block (74) is fixedly connected to the cover plate (2), a shifting block (7) is rotatably connected to the heat-insulation tank (1), two L-shaped rods (71) are symmetrically and rotatably connected to the shifting block (7), two cylinders (72) are fixedly connected to the two L-shaped rods (71), an n-shaped rod (73) is arranged on each cylinder (72), two ends of the n-shaped rod (73) are respectively and correspondingly connected to the two cylinders (72) in a sliding manner, inserting rods (75) are respectively and slidably connected to the two cylinders (72), a plurality of inserting grooves matched with the inserting rods (75) are formed in the n-shaped rod (73), a limiting ring (77) is fixedly connected to one end of each inserting rod (75) far away from the n-shaped rod (73), a first tension spring (76) is sleeved on each inserting rod (75), two ends of the first tension spring (76) are respectively and fixedly connected to the cylinders (72) and the limiting ring (77), a second tension spring (78) is arranged in the cylinder (72), and two ends of the second tension spring (78) are fixedly connected to the n-shaped rod (73) and the cylinder (72) respectively.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN202210721567.2A CN114868738A (en) | 2022-06-24 | 2022-06-24 | Stem cell storage system with temperature monitoring device |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210721567.2A CN114868738A (en) | 2022-06-24 | 2022-06-24 | Stem cell storage system with temperature monitoring device |
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| CN202210721567.2A Pending CN114868738A (en) | 2022-06-24 | 2022-06-24 | Stem cell storage system with temperature monitoring device |
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| CN213503935U (en) * | 2020-09-10 | 2021-06-22 | 重庆贝纳吉液氮生物容器有限公司 | Biological product storage tank |
| CN214962230U (en) * | 2021-01-26 | 2021-12-03 | 上海明悦医疗科技有限公司 | Cold source device |
| CN114013827A (en) * | 2021-10-27 | 2022-02-08 | 冰山松洋生物科技(大连)有限公司 | Self-sustaining liquid nitrogen type biological preservation container |
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| CN109296788A (en) * | 2018-11-17 | 2019-02-01 | 大连悦迈科技服务有限公司 | Exhaust apparatus |
| CN110778717A (en) * | 2019-10-31 | 2020-02-11 | 合肥化机装备制造有限公司 | Cylinder cover locking mechanism for vertical pressure container |
| CN212416501U (en) * | 2020-03-19 | 2021-01-29 | 耿晴晴 | Respiratory tract negative pressure aspirator for field emergency nursing |
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Application publication date: 20220809 |