CN114849274A - Hydrogen protection extraction method and device for plants rich in easily-oxidized active ingredients - Google Patents
Hydrogen protection extraction method and device for plants rich in easily-oxidized active ingredients Download PDFInfo
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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- B01D11/00—Solvent extraction
- B01D11/02—Solvent extraction of solids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D11/00—Solvent extraction
- B01D11/02—Solvent extraction of solids
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- B—PERFORMING OPERATIONS; TRANSPORTING
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- B01D—SEPARATION
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- B01D29/01—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor with flat filtering elements
- B01D29/03—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor with flat filtering elements self-supporting
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/08—Processes employing the direct application of electric or wave energy, or particle radiation; Apparatus therefor
- B01J19/10—Processes employing the direct application of electric or wave energy, or particle radiation; Apparatus therefor employing sonic or ultrasonic vibrations
Abstract
A hydrogen protection extraction method and device for plants rich in easily-oxidizable active ingredients are disclosed, wherein the extraction method comprises introducing hydrogen during the extraction process; the extraction device comprises an extraction tank, and a liquid circulation pipeline and a gas circulation pipeline are arranged on the extraction tank; the extraction method under hydrogen protection improves the content of the active ingredients which are easy to oxidize in the plant extract, improves the biological activity of the extract, and greatly improves the quality, the raw material application rate and the extraction efficiency of the extract; the invention has wide range of extractable plants, covers most of herbaceous plants, such as tea, fruits, Chinese herbal medicines and the like; can be combined with various existing auxiliary extraction technologies to generate protection efficacy; the extraction method has the advantages of wide application range, strong flexibility, improved product quality, improved extraction rate, and reduced production cost.
Description
Technical Field
The invention relates to the field of plant extraction, in particular to a hydrogen protection extraction method and a hydrogen protection extraction device for plants rich in easily-oxidized active ingredients.
Background
The plant extract is a product formed by extracting effective components from plants (all or a part of the plants) by a physicochemical extraction and separation process by adopting a proper solvent or method according to the requirements of the application of the extracted final product without changing the structure of the effective components. The plant extract has wide market, and is widely applied to the fields of medicines, foods, health products, cosmetics, feeds and the like, so that the development of a large number of related industries is driven and supported. With the continuous development of plant extraction technology and the continuous emphasis of people on green, ecological and natural products, plant extracts, natural foods and the like become the current hot topics. In addition, as the living standard of people is gradually improved, the attention to health is not limited to the treatment of diseases, but the defense of diseases and the health care of the people are more important, so that the natural health care food with functions, which is prepared by taking various food-bearing plants (including fruits, vegetables and the like, particularly some varieties with small planting regions, strict condition requirements, strong seasonality, difficult storage or scarce resources) and extracts of the food-bearing plants as raw materials, is subjected to unprecedented attention in the world, and the market demand is continuously increased. In addition, the treasure traditional Chinese medicine (including proved recipes such as single recipe and compound recipe) in the traditional Chinese medicine needs to be internationalized and modernized, and also needs to rely on a plant extraction method and a plant extraction process of the Chinese herbal medicine to retain the active ingredients and the efficacy of the plants. With the continuous development of modern technology of plant extraction technology, certain achievements are obtained by optimizing the extraction and separation of plants by using modern scientific technology and method, but today's plant extracts still face the dilemma that the structure, content and efficacy of components in the plants cannot be completely reserved and reduced. In the process of plant extraction, the extraction method and technology are crucial to the activity and function of the final extract, the extraction time, temperature, solvent and environmental factors have a great influence on the extraction and effective rate of the plant active ingredients, and the success of extraction can directly influence the effect of the finally obtained plant extract raw material applied to health care products, cosmetics, medicines, feeds and the like. Therefore, there is a constant effort to improve and optimize extraction techniques. The existing plant extraction methods can be divided into traditional extraction methods and modern extraction methods, and the traditional extraction methods mainly comprise a decoction method, an immersion method, a reflux method, a percolation method and the like; the modern plant extraction method mainly comprises a supercritical fluid extraction process, an enzyme extraction process, a flash extraction process, an ultrafiltration extraction process, a microwave extraction process, an ultrasonic extraction process and the like. Modern extraction methods are superior to traditional extraction methods, but both have the defects of low efficiency, waste of raw materials and the like to a certain extent, and particularly, the oxidation of effective components in plants is serious, so that huge loss is caused. The method has the advantage of using nitrogen as inert gas to carry out anti-oxidation protection on the extraction process, but the actual effect is not ideal, and the plants and the extracts can be oxidized and browned. Therefore, research on better antioxidant protection technology is needed to improve the utilization rate of plant raw materials and the quality of extracts.
Hydrogen is an important industrial raw material and a green energy carrier, people think that hydrogen is inert to organisms in the past, and recently newly discover that hydrogen has wide biological activity, is a biological gas signal molecule and can play physiological regulation activity and a signal transduction function in organisms. Recent researches find that hydrogen can prolong the storage period of picked fruits and vegetables, keep cut flowers fresh and keep raw meat or eggs fresh due to multiple effects of oxidation resistance and the like, and a patent (CN 107372779A) mixes hydrogen with carbon monoxide, carbon dioxide and oxygen for keeping fresh meat; in the patent (CN 107373279A), hydrogen is mixed with carbon dioxide, water vapor and oxygen for keeping the rhizoma gastrodiae fresh; the patent (CN111406789A) mixes hydrogen, nitrogen and carbon dioxide for preserving eggs. However, no report of applying hydrogen to plant extraction is found at present.
Disclosure of Invention
Aiming at the defect that the effective components of plants are seriously damaged by oxidation in the extraction process by the existing extraction method, and combining the recently recognized hydrogen biological effect, the invention aims to provide the hydrogen protection extraction method and the device for the plants rich in the easily-oxidized active components.
In order to achieve the purpose, the technical scheme of the invention is as follows:
the hydrogen protection extraction method of the plant rich in the easily oxidized active ingredient is characterized in that hydrogen is introduced in the extraction process.
The extraction process comprises microwave extraction, flash extraction and ultrasonic-assisted extraction.
The plants rich in the easily-oxidized active ingredients comprise herbaceous plants, tea, fruits and Chinese herbal medicines.
The plant rich in easily oxidizable active ingredients comprises green tea, black tea, oolong tea, white tea, yellow tea, dark tea, cherry, blueberry, grape, ginseng, ganoderma lucidum, polygonum multiflorum, medlar, chrysanthemum or gynostemma pentaphylla.
The invention also provides a hydrogen protection extraction device rich in plants with easily-oxidized active ingredients, which comprises an extraction tank 2, wherein the extraction tank 2 is provided with a liquid circulation pipeline 3 and a gas circulation pipeline 4;
the inlet of the liquid circulation pipeline 3 is connected with a liquid filter 65 arranged at the bottom of the extraction tank 2, a circulating liquid pump 31 is arranged on the liquid circulation pipeline 3, the outlet of the liquid circulation pipeline 3 is connected with an atomizing nozzle 11 arranged at the top of the extraction tank 2, and the nozzle of the atomizing nozzle 11 is positioned inside the extraction tank 2; the inlet of the liquid circulation pipeline 3 is also connected with a solvent adding pipeline 43 in parallel;
the inlet of the gas circulation pipeline 4 is communicated with a gas filter 62 above the interior of the extraction tank 2, a circulation gas pump 41 and a hydrogen cylinder 1 are arranged on the gas circulation pipeline 4 in parallel, the outlet of the gas circulation pipeline 4 is connected with an atomizing nozzle 11, the inlet of the gas circulation pipeline 4 is also connected with a vacuum pumping pipeline 5 in parallel, and a vacuum pump 51 is arranged on the vacuum pumping pipeline 5;
the top of the extraction tank 2 is provided with a vacuum pressure gauge 9 and a hydrogen pressure gauge 10 for monitoring the conditions in the tank.
And a hydrogen concentration detection device 39 is arranged in the extraction tank 2.
And a filter screen 61 is arranged below the inner part of the extracting tank 2.
The bottom of the extracting tank 2 is provided with a tap 8, the inlet of the tap 8 is arranged in the extracting tank 2, the outlet of the tap 8 is arranged outside the extracting tank 2, and the inlet and the outlet of the tap 8 are respectively provided with a filter 63 and a filter 64.
The outer surface of the bottom of the extraction tank 2 is provided with an ultrasonic device 7.
The extraction method based on the hydrogen protection extraction device rich in the easily-oxidized active ingredient plants comprises the following steps:
firstly, placing plant raw materials on a filter screen 61 in an extraction tank 2;
step two, vacuumizing the interior of the extraction tank 2 through a vacuum pump 51;
step three, after the interior of the extraction tank 2 is in a vacuum state, turning on the ultrasonic device 7, simultaneously turning on the circulating liquid pump to enable the solvent to pass through the liquid circulating pipeline 3 from the filter 65 to the atomizing spray head 11 at the top end of the extraction tank 2, turning on the circulating air pump to enable the hydrogen to pass through the gas circulating pipeline 4 from the filter 62 to the atomizing spray head 11 at the top end of the extraction tank 2, at the moment, the solvent and the hydrogen are converged, the solvent and the hydrogen are sprayed into the extraction tank in an umbrella shape in an atomizing state through the porous atomizing spray head 11, and the solvent below the interior of the extraction tank 2 is continuously circulated and pumped to the atomizing spray head 11 at the top end of the extraction tank 2, so that the concentration of the solvent is more uniform; during the extraction process, the gas pressure and the gas volume ratio are controlled by a hydrogen pressure gauge 10, so that hydrogen can be supplemented, the pressure in the extraction tank 2 is always kept within 1-1.5 atmospheric pressures, and the volume fraction of the hydrogen is beyond the explosion limit;
and step four, when the extraction is finished, closing the ultrasonic device 7 and the circulating pump, unscrewing the tap 8, and taking the extracting solution through an external pipeline.
The solvent comprises water.
The invention has the advantages that:
(1) the hydrogen-protected extraction method improves the content of the active ingredients which are easy to oxidize in the plant extract, improves the biological activity of the extract, and greatly improves the quality, the raw material application rate and the extraction efficiency of the extract.
(2) The hydrogen-protected extraction method has a wide range of extractable plants, and covers most of herbaceous plants, such as tea (green tea, black tea, oolong tea, white tea, yellow tea, black tea and the like), fruits (cherry, blueberry, grape seeds and the like), Chinese herbal medicines (ginseng, lucid ganoderma, polygonum multiflorum, medlar) and the like. The present invention is not limited to the experimental examples (chrysanthemum, gynostemma pentaphyllum); the extraction method of hydrogen protection can be combined with various existing auxiliary extraction technologies to generate protection effects, such as microwave extraction technology, flash extraction technology and the like. Without limitation, the ultrasound-assisted extraction technique employed in the present invention.
(3) The atomization spray head 11 of the invention enables the solvent and the hydrogen to spray in an umbrella shape in an atomization state to the extraction tank, the liquid is in the atomization state, the dissolution amount of the hydrogen in the liquid can be enhanced, the hydrogen is saved in a circulation mode, the solvent and the hydrogen are continuously dissolved, and the antioxidation protection effect of the hydrogen on the plant extraction process is enhanced.
(4) The arrangement of the gas and liquid circulating pipelines ensures that the solvent and the gas below the extraction tank 2 are continuously circulated and pumped to the atomizing spray head 11 at the top end of the extraction tank 2, so that the concentration and the temperature of the solvent are more uniform.
(5) The ultrasonic device 7 is arranged, the formed shock waves and the micro-jet can form a cavitation effect, so that cells are broken, the reaction activity of reaction molecules is preserved and improved to the maximum extent, the penetrating power and the transmission capability of cell contents are improved, the movement frequency and the movement speed of substance molecules are increased, and the leaching rate of effective components is improved.
Drawings
Fig. 1 is a schematic structural view of the present invention.
Fig. 2 is a rear view of the extraction tank of the present invention.
Figure 3 is a schematic upper view of an extraction tank of the present invention.
Fig. 4 is a perspective view of an extraction tank of the present invention.
FIG. 5 is a schematic diagram comparing the quantitative analysis of luteolin from a chrysanthemum extract under different gas extraction conditions.
FIG. 6 is a diagram comparing the quantitative analysis of chlorogenic acid in chrysanthemum extract under different gas extraction conditions.
FIG. 7 is a graph showing the comparison of the quantitative analysis of rutin in Gynostemma pentaphyllum extract under different gas extraction conditions.
FIG. 8 is a graph showing the quantitative analysis of chlorogenic acid in Gynostemma pentaphyllum extract comparing different gas extraction conditions.
FIG. 9 is a graph comparing the toxic effect of chrysanthemum extract on HAEC cells under different gas extraction conditions.
FIG. 10 is a graph comparing the effect of chrysanthemum extract on IL-6 content under different gas extraction conditions.
FIG. 11 is a graph comparing the effect of chrysanthemum extract on MMP-1 content under different gas extraction conditions.
FIG. 12 is a graph taken through H 2 DCFDA staining compares the effect of chrysanthemum extract on ROS levels under different gas extraction conditions.
FIG. 13 is a comparison of the effect of chrysanthemum extract on ROS levels by DHE staining under different gas extraction conditions.
The icons in fig. 1, 2, 3 and 4: 1-hydrogen gas cylinder; 2-an extraction tank; 3-a liquid circulation line; 4-a gas circulation line; 5-vacuum pumping pipeline; 31-a circulating liquid pump; 41-circulating air pump; 51-a vacuum pump; 61-a filter screen; 62-a gas filter; 63-a filter; 64-a filter; 65-liquid filters; 7-an ultrasonic device; 8-tap; 9-vacuum pressure gauge; 10-hydrogen pressure gauge; 11-an atomizing spray head; 39-hydrogen concentration detection means; 43-the solubilizer line.
Detailed Description
The present invention will be described in detail below with reference to the accompanying drawings.
Referring to fig. 1-4, the extraction device for plants rich in easily oxidizable active ingredients under the protection of hydrogen comprises an extraction tank 2, wherein a liquid circulation pipeline 3 and a gas circulation pipeline 4 are arranged on the extraction tank 2;
the inlet of the liquid circulation pipeline 3 is connected with a liquid filter 65 arranged at the bottom of the extraction tank 2, a circulating liquid pump 31 is arranged on the liquid circulation pipeline 3, the outlet of the liquid circulation pipeline 3 is connected with an atomizing nozzle 11 arranged at the top of the extraction tank 2, and the nozzle of the atomizing nozzle 11 is positioned inside the extraction tank 2; the inlet of the liquid circulation pipeline 3 is also connected with a solvent adding pipeline 43 in parallel;
the inlet of the gas circulation pipeline 4 is communicated with a gas filter 62 above the interior of the extraction tank 2, a circulation gas pump 41 and a hydrogen cylinder 1 are arranged on the gas circulation pipeline 4 in parallel, the outlet of the gas circulation pipeline 4 is connected with an atomizing nozzle 11, the inlet of the gas circulation pipeline 4 is also connected with a vacuum pumping pipeline 5 in parallel, and a vacuum pump 51 is arranged on the vacuum pumping pipeline 5;
the top of the extraction tank 2 is provided with a vacuum pressure gauge 9 and a hydrogen pressure gauge 10 for monitoring the conditions in the tank.
And a hydrogen concentration detection device 39 is arranged in the extraction tank 2.
And a filter screen 61 is arranged below the inner part of the extracting tank 2.
The bottom of the extracting tank 2 is provided with a tap 8, the inlet of the tap 8 is arranged in the extracting tank 2, the outlet of the tap 8 is arranged outside the extracting tank 2, and the inlet and the outlet of the tap 8 are respectively provided with a filter 63 and a filter 64.
The outer surface of the bottom of the extraction tank 2 is provided with an ultrasonic device 7. The ultrasonic extraction has the advantages of high extraction efficiency, short extraction time, high activity of effective components and the like. Ultrasonic extraction is a separation method with extremely strong physical and acoustic chemical effects, and shock waves and micro-jet formed in a solution can form a cavitation effect to break cells and preserve and improve the reaction activity of reaction molecules to the maximum extent so as to improve the penetrating power and the transmission capability of cell contents, increase the motion frequency and the speed of substance molecules and improve the leaching rate of effective components.
The extraction method of the extraction device based on the plants rich in the easily-oxidized active ingredients under the protection of the hydrogen comprises the following steps:
firstly, placing plant raw materials on a filter screen 61 in an extraction tank 2;
step two, vacuumizing the extraction tank 2 through a vacuum pump 51 to prevent oxygen in the air from oxidizing the raw materials; the vacuum pressure gauge 9 can detect the vacuum degree;
step three, after the interior of the extraction tank 2 is in a vacuum state; the ultrasonic device 7 is started, the circulating liquid pump is started at the same time, so that the solvent passes through the liquid circulating pipeline 3 from the filter 65 to the atomizing spray head 11 at the top end of the extraction tank 2, the circulating air pump is started to enable the hydrogen to pass through the gas circulating pipeline 4 from the filter 62 to the atomizing spray head 11 at the top end of the extraction tank 2, at the moment, the solvent and the hydrogen are converged, the porous atomizing spray head 11 enables the solvent and the hydrogen to be sprayed into the extraction tank in an umbrella shape in an atomizing state, the dissolving amount of the hydrogen in the liquid can be enhanced, the hydrogen is saved and the solvent and the hydrogen are continuously dissolved in a circulating mode, the antioxidation protection effect of the hydrogen on the plant extraction process is enhanced, the solvent below the extraction tank 2 is continuously circulated and pumped to the atomizing spray head 11 at the top end of the extraction tank 2, and the concentration of the solvent is more uniform; during the extraction process, the gas pressure and the gas volume ratio are controlled by a hydrogen pressure gauge 10, so that hydrogen can be supplemented, the pressure in the extraction tank 2 is always kept within 1-1.5 atmospheric pressures, and the volume fraction of the hydrogen is beyond the explosion limit;
and step four, closing the ultrasonic device 7 and the circulating pump when the extraction is finished, and unscrewing the tap 8 to take the extracting solution. Draw 2 inside filter screens and the filter of being provided with of jar, can not prolong circulating liquid pump and circulating air pump life because of impurity blocks up the pipeline when making liquid and gas circulation, the both sides all set up the filter inside and outside tap 8, play multiple filtration impurity's effect when connecing and getting the extract.
The invention can improve the existing extraction device or design a new extraction device and add the extraction method of the invention for hydrogen protection, thereby playing a role of protection. The extraction device is not limited to be applied to the extraction device designed by the invention, and the structure and the mode of circulation of the solvent and the hydrogen gas adopted in the extraction device designed by the invention are not limited to realize the hydrogen protection extraction.
The technical scheme of the invention is applied to the extraction of chrysanthemum and gynostemma pentaphylla.
Taking chrysanthemum (chrysanthemum morifolium ramat) and gynostemma pentaphylla which are plants rich in oxidizable active ingredients as examples, respectively, extracting the two plants in air, nitrogen and hydrogen systems. The method comprises the steps of quantitatively detecting easily-oxidized active ingredients of an extract obtained under an air, nitrogen and hydrogen system by using a liquid chromatography-mass spectrometry method, detecting two easily-oxidized active ingredients, namely luteolin and chlorogenic acid, with abundant content in Hangzhou white chrysanthemum, and quantitatively detecting two easily-oxidized active ingredients, namely rutin and chlorogenic acid, with abundant content in gynostemma pentaphylla, so that the content of the easily-oxidized active ingredients in the extract can be improved by hydrogen protection extraction.
Taking chrysanthemum (chrysanthemum morifolium ramat) as an example, the protection effect of the plant extract on the palmitic acid induced endothelial function damage under the hydrogen protection condition is researched, and the biological activity of the extract obtained by hydrogen protection extraction is proved to be stronger.
Specific experimental operations:
extraction under air conditions: respectively grinding flos Chrysanthemi and herba Gynostemmatis into powder, and degassing water by ultrasonic method. Setting the material-liquid ratio of the Hangzhou white chrysanthemum to be 1 g: 30mL (namely the ratio of the raw material to the solvent), and carrying out ultrasonic extraction at normal temperature for 20 min; the material-liquid ratio of the gynostemma pentaphylla is set to be 1 g: 10mL (namely the ratio of the raw material to the solvent), and ultrasonic extraction is carried out for 30min at normal temperature.
Extracting under the condition of nitrogen protection: grinding Hangzhou white chrysanthemum and gynostemma pentaphylla into powder particles respectively, degassing water by an ultrasonic method, introducing nitrogen, setting the material-liquid ratio of the Hangzhou white chrysanthemum to be 1 g: 30mL (namely the ratio of raw materials to solvent), and performing ultrasonic extraction at normal temperature for 20 min; setting the material-liquid ratio of the gynostemma pentaphylla to be 1 g: 10mL (namely the ratio of the raw material to the solvent), and carrying out ultrasonic extraction at normal temperature for 30 min.
Extracting under the hydrogen protection condition: respectively grinding Hangzhou white chrysanthemum and gynostemma pentaphylla into powder particles, degassing water by an ultrasonic method, and extracting by the method, wherein the Hangzhou white chrysanthemum is subjected to ultrasonic extraction at normal temperature for 20min, and the material-liquid ratio is 1g to 30mL (namely the ratio of raw materials to solvent); setting the material-liquid ratio of the gynostemma pentaphylla to be 1 g: 10mL (namely the ratio of the raw material to the solvent), and carrying out ultrasonic extraction at normal temperature for 30 min.
After repeated experiments, the extract was quantitatively determined using LC-MS technique. The analysis results in fig. 5, 6, 7 and 8 show that the content sequence of the easily oxidizable active ingredients under different extraction conditions is as follows: hydrogen > nitrogen > air. Although nitrogen is an inert gas which is not easy to generate chemical reaction with other substances, and the substances are not easy to oxidize at places with high nitrogen concentration, experimental data analysis finds that hydrogen extraction is superior to nitrogen extraction, and proves that hydrogen has stronger antioxidant protection effect on the extract than nitrogen, and the content of the easily-oxidized active ingredients extracted by hydrogen is the largest.
Next, taking chrysanthemum (chrysanthemum morifolium ramat) extract as an example, the biological activity of the extract obtained by hydrogen protection extraction is researched and verified.
Firstly, whether the chrysanthemum extract is non-toxic to HAEC cells is detected by an MTT method, and the HAEC cells are pretreated for 24h by the chrysanthemum extracts with different concentration grades extracted by an air group, a nitrogen group and a hydrogen group with concentration gradients respectively, as shown in figure 9, the chrysanthemum extracts of all groups have no cell damage effect and do not show toxicity.
Because the inflammatory response of endothelial cells is a typical feature of endothelial cell injury, and the development of the inflammatory response of endothelial cells is the pathological basis for the development of cardiovascular diseases. Comparing the pre-protection effect of chrysanthemum extract prepared under the three gas conditions of air, nitrogen and hydrogen on endothelial cells respectively, after HAEC cells are treated with palmitic acid for 24 hours, the results of fig. 10 and 11 show that the contents of two inflammation indexes of IL-6 level and MMP-1 level are increased, and the endothelial cells are pre-protected by chrysanthemum extract obtained under the three gas systems of air, nitrogen and hydrogen respectively with different concentrations, so that the increase of IL-6 and MMP-1 content caused by palmitic acid can be reduced, and the chrysanthemum extract under the hydrogen protection condition has better anti-inflammatory effect than the chrysanthemum extract obtained under the air or nitrogen condition.
In endothelial cells, the inflammatory response may occur as a result of oxidative stress, and we further examined changes in ROS. H of FIG. 12 2 DCFDA staining method shows that after HAEC cells are treated by palmitic acid for 24 hours, ROS levels in the cells are measured by three different detection methods, and the ROS levels in a model group are obviously increased compared with those in a control group, and after the HAEC cells are pre-protected by chrysanthemum extract, the chrysanthemum extract of nitrogen and hydrogen groups can obviously inhibit the generation of excessive ROS caused by the palmitic acid, but the effect of an air group is not achieved. FIG. 13 shows that the DHE staining method can be used to obtain that the hydrogenome can protect HAEC cells by reducing palmitic acid-induced ROSThe effect of (c) is most remarkable.
The experimental results prove that the plant extraction method under the hydrogen protection condition can effectively enhance the content of the active ingredients easy to oxidize and improve the biological activities of the extract, such as anti-inflammation, antioxidation and the like, and the substances extracted by the method are safe and nontoxic when applied to products, such as food, cosmetics and the like.
Claims (10)
1. The hydrogen protection extraction method of the plant rich in the easily oxidized active ingredient is characterized in that hydrogen is introduced in the extraction process.
2. The method for extracting the plants rich in the oxidizable active ingredient with the hydrogen protection effect according to claim 1, wherein the extraction process comprises microwave extraction, flash extraction and ultrasonic-assisted extraction.
3. The method for extracting hydrogen by protecting active ingredient plant rich in easy oxidation as claimed in claim 1, wherein the active ingredient plant rich in easy oxidation comprises herbs, tea, fruits, and Chinese herbal medicines.
4. The method for extracting a plant rich in an easily oxidizable active ingredient under hydrogen protection according to claim 1, wherein the plant rich in an easily oxidizable active ingredient comprises green tea, black tea, oolong tea, white tea, yellow tea, black tea, cherry, blueberry, grape, ginseng, ganoderma lucidum, polygonum multiflorum, lycium barbarum, chrysanthemum or gynostemma pentaphyllum.
5. The extraction device related to the hydrogen protection extraction method of the plant rich in the easily oxidizable active ingredient according to claim 1, characterized by comprising an extraction tank (2), wherein the extraction tank (2) is provided with a liquid circulation pipeline (3) and a gas circulation pipeline (4);
an inlet of the liquid circulation pipeline (3) is connected with a liquid filter (65) arranged at the bottom of the extraction tank (2), a circulating liquid pump (31) is arranged on the liquid circulation pipeline (3), an outlet of the liquid circulation pipeline (3) is connected with an atomizing nozzle (11) arranged at the top of the extraction tank (2), and a nozzle of the atomizing nozzle (11) is positioned inside the extraction tank (2); the inlet of the liquid circulation pipeline (3) is also connected with a solvent adding pipeline (43) in parallel;
the inlet of the gas circulation pipeline (4) is communicated with a gas filter (62) above the interior of the extraction tank (2), a circulation gas pump (41) and a hydrogen cylinder (1) are connected in parallel on the gas circulation pipeline (4), the outlet of the gas circulation pipeline (4) is connected with an atomizing nozzle (11), the inlet of the gas circulation pipeline (4) is also connected in parallel with a vacuum pumping pipeline (5), and a vacuum pump (51) is arranged on the vacuum pumping pipeline (5);
the top of the extraction tank (2) is provided with a vacuum pressure gauge (9) and a hydrogen pressure gauge (10) for monitoring the conditions in the tank.
6. The hydrogen protection extraction device for the plants rich in the oxidizable active ingredient according to claim 5, wherein a hydrogen concentration detection device (39) is arranged in the extraction tank (2).
7. The hydrogen protection extraction device for plants rich in oxidizable active ingredients of claim 5, characterized in that a filter screen (61) is arranged below the inside of the extraction tank (2).
8. The hydrogen protection extraction device for plants rich in oxidizable active ingredients according to claim 5, characterized in that the bottom of the extraction tank (2) is provided with a faucet (8), the inlet of the faucet (8) is arranged in the extraction tank (2), the outlet of the faucet (8) is arranged outside the extraction tank (2), and the inlet and outlet of the faucet (8) are respectively provided with filters (63, 64).
9. The hydrogen protection extraction device for the plants rich in the easily oxidizable active ingredient according to claim 5, characterized in that the outer surface of the bottom of the extraction tank (2) is provided with an ultrasonic device (7).
10. The extraction method of the hydrogen protection extraction device rich in the easily oxidizable active ingredient plant according to claim 5, is characterized by comprising the following steps:
firstly, placing plant raw materials on a filter screen (61) in an extraction tank (2);
step two, vacuumizing the extraction tank (2) through a vacuum pump (51);
step three, after the interior of the extraction tank (2) is in a vacuum state, turning on an ultrasonic device (7), turning on a circulating liquid pump at the same time, enabling a solvent to pass through a liquid circulating pipeline (3) from a filter (65) to an atomizing nozzle (11) at the top end of the extraction tank (2), turning on a circulating air pump, enabling hydrogen to pass through a gas circulating pipeline (4) from a filter (62) to the atomizing nozzle (11) at the top end of the extraction tank (2), enabling the solvent and the hydrogen to converge at the moment, enabling the solvent and the hydrogen to be sprayed into the extraction tank in an umbrella shape in an atomizing state through a porous atomizing nozzle (11), enabling the solvent to comprise water, and enabling the solvent below the interior of the extraction tank (2) to be continuously and circularly pumped to the atomizing nozzle (11) at the top end of the extraction tank (2) so that the concentration of the solvent is more uniform; in the extraction process, the gas pressure and the gas volume ratio are controlled by a hydrogen pressure gauge (10), so that hydrogen can be supplemented, the pressure in the extraction tank (2) is always kept within 1-1.5 atmospheric pressures, and the volume fraction of the hydrogen is beyond the explosion limit;
and step four, when the extraction is finished, closing the ultrasonic device (7) and the circulating pump, unscrewing the tap (8), and receiving the extracting solution through an external pipeline.
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