CN114847304A - Method for improving drought resistance of plants by using insecticidal fungi - Google Patents
Method for improving drought resistance of plants by using insecticidal fungi Download PDFInfo
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- CN114847304A CN114847304A CN202210633169.5A CN202210633169A CN114847304A CN 114847304 A CN114847304 A CN 114847304A CN 202210633169 A CN202210633169 A CN 202210633169A CN 114847304 A CN114847304 A CN 114847304A
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- insecticidal
- drought resistance
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- drought
- fungi
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- 241000233866 Fungi Species 0.000 title claims abstract description 42
- 230000000749 insecticidal effect Effects 0.000 title claims abstract description 28
- 238000000034 method Methods 0.000 title claims abstract description 16
- 241000190633 Cordyceps Species 0.000 claims abstract description 13
- 244000299906 Cucumis sativus var. sativus Species 0.000 claims 1
- 240000008067 Cucumis sativus Species 0.000 abstract description 30
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 abstract description 22
- 241000196324 Embryophyta Species 0.000 abstract description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 7
- 239000002689 soil Substances 0.000 abstract description 3
- 238000012272 crop production Methods 0.000 abstract description 2
- 239000002917 insecticide Substances 0.000 abstract description 2
- 230000002538 fungal effect Effects 0.000 abstract 1
- 230000008641 drought stress Effects 0.000 description 8
- 230000000694 effects Effects 0.000 description 6
- 235000009849 Cucumis sativus Nutrition 0.000 description 5
- 241000607479 Yersinia pestis Species 0.000 description 4
- 241000235503 Glomus Species 0.000 description 3
- 244000269722 Thea sinensis Species 0.000 description 3
- 235000006468 Thea sinensis Nutrition 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 241000723347 Cinnamomum Species 0.000 description 2
- 208000005156 Dehydration Diseases 0.000 description 2
- 240000004658 Medicago sativa Species 0.000 description 2
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 description 2
- 230000000967 entomopathogenic effect Effects 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 240000002066 Amorpha fruticosa Species 0.000 description 1
- 235000004047 Amorpha fruticosa Nutrition 0.000 description 1
- 241001124076 Aphididae Species 0.000 description 1
- 241000254127 Bemisia tabaci Species 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 102000016938 Catalase Human genes 0.000 description 1
- 108010053835 Catalase Proteins 0.000 description 1
- 241001414720 Cicadellidae Species 0.000 description 1
- 241000250502 Claroideoglomus lamellosum Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241001123597 Funneliformis mosseae Species 0.000 description 1
- 239000008118 PEG 6000 Substances 0.000 description 1
- 240000002390 Pandanus odoratissimus Species 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- 229920002584 Polyethylene Glycol 6000 Polymers 0.000 description 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
- 102000019197 Superoxide Dismutase Human genes 0.000 description 1
- 108010012715 Superoxide dismutase Proteins 0.000 description 1
- 241000357401 Tolypocladium japonicum Species 0.000 description 1
- 244000042324 Trifolium repens Species 0.000 description 1
- 235000013540 Trifolium repens var repens Nutrition 0.000 description 1
- 240000001417 Vigna umbellata Species 0.000 description 1
- 235000011453 Vigna umbellata Nutrition 0.000 description 1
- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
- 235000012333 Vitis X labruscana Nutrition 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 235000014787 Vitis vinifera Nutrition 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000000065 osmolyte Effects 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 230000003032 phytopathogenic effect Effects 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/05—Fruit crops, e.g. strawberries, tomatoes or cucumbers
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P21/00—Plant growth regulators
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Botany (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- General Chemical & Material Sciences (AREA)
- Mycology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Agronomy & Crop Science (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Virology (AREA)
- Dentistry (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a method for improving drought resistance of plants by using insecticidal fungi. The crop production is always threatened by drought, the drought resistance of crops is improved, and the method is important for saving water resources and reducing the loss caused by drought. The insecticidal fungi widely exist in the nature, and the prior application of the insecticidal fungi mainly focuses on the aspect of insecticidal. Besides the insecticidal action, whether the fungal insecticide can play a role in improving the drought resistance of plants or not. The technology realizes the purpose of improving the drought resistance of cucumber plants by treating and cultivating cucumber soil by using the insecticidal fungus, i.e. the cordyceps javanicus. The invention has direct guidance function for further scientific utilization of the insecticidal fungi and has important significance for improving the drought resistance of crops.
Description
One, the technical field
The invention belongs to the technical field of agriculture, and particularly relates to a method for improving drought resistance of plants by using insecticidal fungi.
Second, technical background
The crop production is always threatened by drought, the drought resistance of crops is improved, and the method is important for saving water resources and reducing the loss caused by drought. The cultivation of drought-resistant varieties is an important way for improving the drought resistance of crops, and besides, other effective methods exist, so that the cultivation method is worthy of research.
Is fungus a ubiquitous microorganism in nature that can play a role in improving drought resistance of crops? Arbuscular mycorrhizal fungi are reported as a type of microorganism capable of forming a mutual-benefiting symbiont with a host plant nutrient root system, namely arbuscular mycorrhizal fungi, and the arbuscular mycorrhizal fungi can improve the water absorption or water retention capacity of cells and improve the water utilization rate of plants by improving the permeability regulation capacity of the host plant, so that the drought resistance capacity of the host plant is improved. Arbuscular mycorrhizal fungi have the function of improving drought resistance on various plants including woody plants, herbaceous plants and the like, for example, Chen Jie and the like (2014) find that the arbuscular mycorrhizal fungi can improve the mass fractions of proline, soluble sugar and soluble protein of amorpha fruticosa leaves under the water stress and improve the drought resistance of the arbuscular mycorrhizal fungi; the xu Pinghui et al (2017) find that the Glomus versiforus, Glomus intraradiculosa and Glomus mosseae remarkably improve the biomass of tea plant, also enhance the activity of superoxide dismutase, peroxidase and catalase, and also can relieve the damage of water stress on the tea plant and promote the growth of the tea plant; liuhuan et al (2018) further reported that Glomus lamellosum and Glomus etuniticam, which belong to arbuscular mycorrhizal fungi, improve the enzyme activity and the content of penetrating substances in the Cinnamomum migao of trees, increase the water utilization efficiency and improve the drought resistance of the Cinnamomum migao seedlings; arbuscular mycorrhizal fungi can also improve the drought resistance of grape seedlings (pandan et al, 2018); in addition to woody plants, April et al (2014) found that arbuscular mycorrhizal fungi can improve the accumulation of alfalfa osmolyte regulators, and have the effect of improving drought resistance on 3 herbaceous plants including alfalfa, red bean grass and white clover.
The insecticidal fungi are an important microbial insecticidal resource, and the insecticidal function of the insecticidal fungi is widely regarded. In addition to insecticidal effects, entomopathogenic fungi have also been found to have other functions, such as inhibiting phytopathogenic fungi, promoting plant growth, etc. Is the insecticidal fungus capable of increasing drought resistance of plants? No research report is available. Cordyceps javanicus Cordyceps japonica is an important entomopathogenic fungus, can infect various piercing-sucking pests such as bemisia tabaci, aphids, leafhoppers and the like, can form epidemic diseases in pest populations and effectively control the pests, and is developed into a biological insecticide product. In order to further develop the function of the Cordyceps javanicus, the research evaluates the influence of the Cordyceps javanicus on the drought resistance of the cucumbers, and a method for improving the drought resistance of the crops by utilizing the insecticidal fungi is expected to be obtained, so that the effect of the insecticidal fungi is fully exerted, the pest control target is realized, and the drought resistance of the crops is improved. The invention has important guiding function for fully exploring and evaluating the functions of the insecticidal fungi.
Third, summary of the invention
1. Objects of the invention
Provides a method for improving the drought resistance of plants by using insecticidal fungi, in particular to a method for improving the drought resistance of cucumber plants by treating cucumber cultivation soil by using insecticidal fungi cordyceps javanicus.
2. Technical scheme
The invention relates to a method for improving drought resistance of plants, which is characterized in that the drought resistance of the plants is improved by utilizing insecticidal fungi.
3. Advantageous effects
Compared with the prior art, the method of the invention has the following beneficial effects: (1) compared with the conventional cultivation of drought-resistant varieties, the method provided by the invention has the advantages that the drought-resistant capability of the plants is improved through the treatment of the insecticidal fungi, and the method is more convenient and faster; (2) the insecticidal fungus has rich resources, and the invention is beneficial to further improving the utilization level of the insecticidal fungus.
Fourth, detailed description of the invention
An embodiment of the invention is a method using an indoor assay.
The measurement method is as follows:
1. cordyceps javanicus processing
Treating cucumber seeds according to the amount of 5 hundred million cordyceps javanica spores per cucumber seed, uniformly stirring the spore powder and nutrient soil, putting the mixture into a disposable plastic cup of 500 ml, planting 1 cucumber seed in each cup for 80 cups in total, simultaneously planting 1 seed in each cup for 80 cups in total, and respectively planting 1 seed in each cup for 1 group of 10 cups and 8 groups of the treatment and the comparison. And placing the planted cucumbers in a lighting incubator for cultivation.
2. Drought treatment
Performing normal watering management on all cucumbers within 14 days after planting, performing drought stress treatment when the cucumbers grow for 14 days, thoroughly watering the cucumbers with 10% PEG-6000 solution, subsequently not watering, performing drought treatment on 4 groups of a control group and a Cordyceps javanicus treatment group which are not treated by the Cordyceps javanicus, and performing normal water management on the other 4 groups. I.e. 4 replicates per treatment group.
3. Determination of drought resistance
Cucumber seedling survival observation is carried out 5 days and 10 days after drought stress treatment, the number of dead seedlings in each group is recorded, the death rate is calculated, and the height and stem thickness of each cucumber seedling are measured by using a ruler at the same time.
Fifthly, implementation effect:
research shows that the insecticidal fungus, Cordyceps javanicus, reduces the mortality rate of cucumber seedlings under drought stress. The mortality rates of the cucumber seedlings treated by the fungus and the control cucumber seedlings are 12.5 percent and 11.25 percent respectively after 5 days of drought stress, the mortality rates of the cucumber seedlings treated by the fungus and the control cucumber seedlings are not significantly different (t is 0.3974 and P is 0.7177), the mortality rate of the cucumber seedlings treated by the fungus is 66.5 percent after 10 days of drought stress, the mortality rate of the cucumber seedlings treated by the fungus and the control cucumber seedlings are 75 percent, and the mortality rates of the cucumber seedlings treated by the fungus and the control cucumber seedlings are significantly different (t is 3.833 and P is 0.0313).
Cordyceps javanicus treatment was also found to be beneficial to cucumber growth under drought stress conditions. The plant heights of the cucumber of the fungus treatment group and the cucumber of the control group are respectively 17cm and 16cm after 10 days of drought stress, the plant heights of the cucumber of the fungus treatment group and the cucumber of the control group which are subjected to normal water management at the same period are respectively 24cm and 21 cm; from the stem thickness, the stem thickness of the fungus-treated group and the control group after 10 days of drought stress was 3.4mm and 1.8mm, respectively, with a significant difference (t ═ 9.118, P < 0.0001).
Therefore, the Cordyceps javanicus can effectively improve the drought resistance of cucumber plants.
Claims (1)
1. A method for utilizing insecticidal fungus to resist high drought resistance of crops is characterized in that insecticidal fungus Cordyceps javanicus is utilized to improve drought resistance of cucumber plants.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210633169.5A CN114847304A (en) | 2022-06-07 | 2022-06-07 | Method for improving drought resistance of plants by using insecticidal fungi |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210633169.5A CN114847304A (en) | 2022-06-07 | 2022-06-07 | Method for improving drought resistance of plants by using insecticidal fungi |
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| CN114847304A true CN114847304A (en) | 2022-08-05 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202210633169.5A Pending CN114847304A (en) | 2022-06-07 | 2022-06-07 | Method for improving drought resistance of plants by using insecticidal fungi |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101514325A (en) * | 2008-11-25 | 2009-08-26 | 华南农业大学 | Isaria javanicanicus mould strain and application thereof in preventing and controlling prodenia litura |
| KR102063801B1 (en) * | 2018-11-01 | 2020-01-08 | 전남대학교 산학협력단 | Preparing method of dibutyl succinate |
| KR102090758B1 (en) * | 2018-11-01 | 2020-03-18 | 전남대학교 산학협력단 | Composition for promoting growth of plant and Method for promoting growth of plant |
| CN111961598A (en) * | 2020-09-18 | 2020-11-20 | 华南农业大学 | Isaria javanicus strain and application thereof in aspect of preventing and treating phyllotreta striolata |
-
2022
- 2022-06-07 CN CN202210633169.5A patent/CN114847304A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101514325A (en) * | 2008-11-25 | 2009-08-26 | 华南农业大学 | Isaria javanicanicus mould strain and application thereof in preventing and controlling prodenia litura |
| KR102063801B1 (en) * | 2018-11-01 | 2020-01-08 | 전남대학교 산학협력단 | Preparing method of dibutyl succinate |
| KR102090758B1 (en) * | 2018-11-01 | 2020-03-18 | 전남대학교 산학협력단 | Composition for promoting growth of plant and Method for promoting growth of plant |
| CN111961598A (en) * | 2020-09-18 | 2020-11-20 | 华南农业大学 | Isaria javanicus strain and application thereof in aspect of preventing and treating phyllotreta striolata |
Non-Patent Citations (1)
| Title |
|---|
| 张延威，韩燕峰，刘讯，梁宗琦: "土壤中产天然维生素 E 真菌新资源的筛选" * |
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