CN114832699A - Cell culture is with shaking mixing arrangement - Google Patents
Cell culture is with shaking mixing arrangement Download PDFInfo
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- CN114832699A CN114832699A CN202210785149.XA CN202210785149A CN114832699A CN 114832699 A CN114832699 A CN 114832699A CN 202210785149 A CN202210785149 A CN 202210785149A CN 114832699 A CN114832699 A CN 114832699A
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- box body
- plate
- bearing seat
- cell culture
- rotating shaft
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- 238000004113 cell culture Methods 0.000 title claims abstract description 29
- 238000002156 mixing Methods 0.000 title claims description 23
- 238000013016 damping Methods 0.000 claims abstract description 17
- 230000000694 effects Effects 0.000 claims abstract description 16
- 230000005540 biological transmission Effects 0.000 claims description 28
- 230000001105 regulatory effect Effects 0.000 claims description 13
- 210000003437 trachea Anatomy 0.000 claims description 13
- 230000006835 compression Effects 0.000 claims description 8
- 238000007906 compression Methods 0.000 claims description 8
- 230000000149 penetrating effect Effects 0.000 claims description 4
- 238000003805 vibration mixing Methods 0.000 abstract description 4
- 230000010355 oscillation Effects 0.000 description 13
- 239000007788 liquid Substances 0.000 description 9
- 230000007246 mechanism Effects 0.000 description 8
- 230000035939 shock Effects 0.000 description 8
- 238000000034 method Methods 0.000 description 5
- 238000010521 absorption reaction Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 230000000903 blocking effect Effects 0.000 description 3
- 239000012531 culture fluid Substances 0.000 description 3
- 230000009471 action Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 1
- 235000017491 Bambusa tulda Nutrition 0.000 description 1
- 241001330002 Bambuseae Species 0.000 description 1
- 241001391944 Commicarpus scandens Species 0.000 description 1
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 239000011425 bamboo Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000012606 in vitro cell culture Methods 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
- B01F31/20—Mixing the contents of independent containers, e.g. test tubes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/40—Mounting or supporting mixing devices or receptacles; Clamping or holding arrangements therefor
- B01F35/42—Clamping or holding arrangements for mounting receptacles on mixing devices
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F2101/00—Mixing characterised by the nature of the mixed materials or by the application field
- B01F2101/44—Mixing of ingredients for microbiology, enzymology, in vitro culture or genetic manipulation
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- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Mixers With Rotating Receptacles And Mixers With Vibration Mechanisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention provides a vibration mixing device for cell culture, which comprises a main box body, wherein a placing hole is formed in a fixed table, an air bag is arranged in the placing hole and communicated with an inflation adjusting device, and an incomplete gear shaft and a first limiting nut are sleeved on a first rotating shaft in a threaded manner; the push plate can be intermittently pushed by a driving device; a second rotating shaft is arranged in the second bearing seat, and a driving gear is fixedly sleeved on the second rotating shaft; the bottom of the main box body is fixedly provided with a bearing seat, the bottom of a bearing groove formed by the damping plate and the bearing seat is connected with a damping spring, and the connecting rod is abutted against the sliding plate. The invention provides a vibration mixing device for cell culture, which can stably clamp test tubes of different specifications, can independently operate certain test tubes to prevent the test tubes from vibrating, can independently adjust the vibration amplitude of different test tubes, automatically stops vibrating when the test tubes are separated, and has better use effect.
Description
Technical Field
The invention relates to the technical field of cell culture, in particular to a shaking and mixing device for cell culture.
Background
At present, in the process of cell culture, a culture bottle or a culture plate is usually shaken manually, so that the aim of uniformly mixing is difficult to achieve, the experimental workload is increased, and the experimental time is prolonged.
In the prior art, a shaking and mixing device for cell culture with publication number "CN 111500450A" and classification number "B01F 11/00" comprises a fixed table, a shaking cylinder, a sample cylinder, a first shaking mechanism and a second shaking mechanism; a rotary table is rotatably arranged on the fixed table through a rotating shaft; the vibration cylinder is fixedly arranged on the rotary table, the sample cylinder is arranged in the vibration cylinder, and two opposite sides of the sample cylinder are provided with support shafts which are rotationally connected with the vibration cylinder; the first oscillating mechanism is arranged on the fixed table and used for driving the rotary table to rotate forward and backward alternately; the second oscillating mechanism is arranged in the oscillating cylinder and used for driving the sample cylinder to oscillate back and forth in the oscillating cylinder, and the oscillating and mixing device for cell culture enables the sample cylinder to rotate back and forth around the central axis of the oscillating cylinder and oscillate back and forth around the central axis of the supporting shaft by arranging the first oscillating mechanism and the second oscillating mechanism, so that the oscillating and mixing effect is improved; this cell culture is with shaking mixing arrangement can shake the mechanism through controlling first shaking mechanism and second, adjusts the sample section of thick bamboo and shakes even amplitude, is favorable to improving cell culture's effect.
However, the method still has the obvious defects in the using process: 1. according to the device, the plurality of test tube slots which penetrate through the test tube rack are arranged on the test tube rack, when the device is used, test tubes can be placed in the test tube slots one by one and then are processed in a unified mode, however, if some test tubes are not matched in size specification or are not placed stably, the test tubes are shaken to be thrown out and broken or broken, and potential safety hazards exist; 2. above-mentioned device can only carry out unified shock to the test tube and handle, but, the volume of different in vitro cell culture liquid is probably different, to the big test tube of culture solution volume, if adopt great amplitude of shaking to vibrate, leads to cell culture liquid to be thrown away easily and cause the pollution, and above-mentioned device can not effectual solution this problem.
Disclosure of Invention
The present invention is directed to a shaking mixing device for cell culture, which solves the above problems of the prior art.
In order to achieve the purpose, the invention provides the following technical scheme:
a vibration mixing device for cell culture comprises a main box body, wherein an auxiliary box body is fixedly arranged on the side face of the main box body, a plurality of positioning blocks are fixedly arranged on the side wall of an inner cavity of the main box body, one sides of the positioning blocks, which are far away from the auxiliary box body, are respectively provided with a first bearing seat in an embedded manner, a first rotating shaft is arranged in the first bearing seats, and a fixing table is fixedly arranged on the side face of each first rotating shaft;
the fixing table is provided with a placing hole, an air bag is arranged in the placing hole and is communicated with the inflation adjusting device, the first rotating shaft is sleeved with an incomplete gear shaft and first limiting nuts in a threaded manner, and the number of the first limiting nuts in each group is two, and the first limiting nuts are respectively arranged on two sides of the incomplete gear shaft;
racks are movably arranged in the main box body and the auxiliary box body in a penetrating mode, a push plate is fixedly arranged at the end portion of the rack positioned in the auxiliary box body, a compression spring is connected between the push plate and the outer wall of the main box body, the push plate can be intermittently pushed by a driving device, one end of the rack positioned in the main box body is movably inserted into a groove of the concave block, and a connecting spring is connected between the rack and the bottom wall of the groove of the concave block;
a guide rail is fixedly arranged in the main box body, a sliding plate is movably arranged in the guide rail, a return spring is connected between the sliding plate and the inner wall of the main box body, a second bearing seat is embedded in one side, away from the return spring, of the sliding plate, a second rotating shaft is arranged in the second bearing seat, a driving gear is fixedly sleeved on the second rotating shaft, a driving bevel gear and a second limiting nut are sleeved on the second rotating shaft in a threaded manner, two second limiting nuts are arranged in each group and are respectively arranged on two sides of the driving bevel gear, a mounting rod is fixedly arranged on the positioning block, a third bearing seat is embedded in the side surface of the mounting rod, a central rod is arranged in the third bearing seat, and a driven bevel gear and a transmission gear are fixedly sleeved on the central rod;
the fixed bearing seat that is provided with in bottom of the main tank body, the embedded piston pipe that is provided with in the bearing seat, the activity is provided with the piston plate in the piston pipe, the fixed extension rod that is provided with on the piston plate, the fixed shock attenuation board that is provided with on the extension rod, the bearing tank bottom connection that shock attenuation board and bearing seat were seted up is provided with damping spring, the piston pipe communicates in the cavity ring, the intercommunication is provided with a plurality of protruding pipes on the cavity ring, the activity is provided with the fly leaf in the protruding pipe, the fixed connecting rod that is provided with on the fly leaf, connecting rod and slide looks butt.
Preferably, aerify adjusting device is including adjusting the trachea, adjust the embedded setting of trachea in the fixed station, just it is linked together with the gasbag to adjust the trachea, it is provided with the regulating plate to adjust the activity in the trachea, the fixed screw rod that is provided with on the regulating plate, the inside fixed shutoff board that is provided with of one end that the gasbag was kept away from to adjust the trachea, set up the screw hole in the shutoff board, the screw rod screw thread sets up in the screw hole, just the fixed handle that is provided with of one end that the regulating plate was kept away from to the screw rod.
Preferably, the driving device comprises a rotating motor, the rotating motor is arranged in the auxiliary box body, a transmission shaft is fixedly arranged on an output shaft of the rotating motor, a cam is fixedly arranged on the transmission shaft, and the cam can intermittently push the push plate when continuously rotating.
Preferably, a third rotating shaft is fixedly arranged on one side, away from the auxiliary box body, of the fixing table at the non-head end, the third rotating shaft is arranged in a fourth bearing seat, and the fourth bearing seat is embedded in the positioning block.
Preferably, an elastic pad is arranged in the supporting groove of the supporting seat.
Preferably, a blocking block is fixedly arranged on the inner wall of the opening end of the protruding pipe, which is far away from one side of the hollow ring.
Compared with the prior art, the invention has the beneficial effects that:
1. according to the test tube clamp, the fixing table is provided with the placing hole, the air bag is arranged in the placing hole and is communicated with the inflation adjusting device, so that even for test tubes with different thicknesses, the air bag can be independently expanded through the inflation adjusting device after being placed, the test tubes can be stably clamped, the test tubes are effectively protected, and the universality is higher;
2. according to the invention, the driving bevel gear and the second limit nuts are sleeved on the second rotating shaft in a threaded manner, two second limit nuts are arranged in each group and are respectively arranged on two sides of the driving bevel gear, when the driving bevel gear is not meshed with the corresponding driven bevel gear, the corresponding fixed table cannot rotate, so that test tubes in the fixed table cannot vibrate, therefore, after a plurality of test tubes are placed in the device, if one or some test tubes do not need to vibrate again, the test tubes do not need to be taken out at the moment, only the driving bevel gear is rotated and moved, the vibration of other test tubes is not influenced, and the taking is more convenient;
3. in the invention, a plurality of test tubes can be placed, under a general condition, the larger the oscillation amplitude is, the better the mixing effect is, but the components of cell culture solution in different test tubes are possibly different, for individual test tubes with large culture solution amount, because the liquid level is high, in order to avoid spilling the cell culture solution, the position of an incomplete gear shaft can be rotationally adjusted at the moment, so that the number of teeth which can be meshed with a transmission gear is reduced, the oscillation amplitude is further reduced, the problem that the cell culture solution is thrown out due to the adoption of larger oscillation amplitude is avoided, pollution is avoided, after the uniform starting, other test tubes with low liquid level can still be greatly oscillated, the integral oscillation mixing effect is better, and the adaptability is strong;
4. when a certain test tube is not clamped stably or loosened after clamping, if the vibration operation is started if the test tube is not clamped, the test tube is broken easily by the fixing table during vibration and swing, and potential safety hazards exist.
The invention provides a vibration mixing device for cell culture, which can stably clamp test tubes of different specifications, can independently operate certain test tubes to prevent the test tubes from vibrating, can independently adjust the vibration amplitude of different test tubes, automatically stops vibrating when the test tubes are separated, and has better use effect.
Drawings
FIG. 1 is a schematic top cross-sectional view of the overall structure of the present invention;
FIG. 2 is an enlarged view taken at A in FIG. 1 according to the present invention;
FIG. 3 is an enlarged view of the invention at B in FIG. 1;
FIG. 4 is a schematic view of a test tube of the present invention in a stationary station relative to a holder;
FIG. 5 is a schematic view of the test tube of the present invention in the holder after being detached from the fixing table;
FIG. 6 is a schematic top view of the raised tubes of the present invention on a hollow ring.
In the figure: 1 main box body, 2 auxiliary box bodies, 3 positioning blocks, 4 first bearing seats, 5 first rotating shafts, 6 fixing tables, 601 placing holes, 7 air bags, 8 adjusting air pipes, 9 adjusting plates, 10 screw rods, 11 plugging plates, 12 threaded holes, 13 handles, 14 incomplete gear shafts, 15 first limiting nuts, 16 racks, 17 push plates, 18 compression springs, 19 rotating motors, 20 transmission shafts, 21 cams, 22 concave blocks, 23 connecting springs, 24 guide rails, 25 sliding plates, 26 restoring springs, 27 second bearing seats, 28 second rotating shafts, 29 driving gear wheels, 30 driving bevel gears, 31 second limiting nuts, 32 mounting rods, 33 third shaft, 34 central rod, 35 driven bevel gears, 36 transmission gears, 37 third rotating shafts, 38 fourth bearing seats, 39 bearing seats, 40 elastic cushions, 41 piston pipes, 42 piston plates, 43 extension rods, 44 damping plates, 45 damping spring bearing seats, 46 hollow ring, 47 protruding tube, 48 movable plate, 49 connecting rod, 50 stop block and 51 test tube.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1 to 6, the present invention provides a technical solution:
the first embodiment is as follows:
the utility model provides a cell culture is with shaking mixing arrangement, including the main tank body 1, the fixed vice box 2 that is provided with in side of main tank body 1, the fixed a plurality of locating pieces 3 that are provided with on the inner chamber lateral wall of main tank body 1, locating piece 3 is kept away from one side of vice box body 2 and all is embedded to be provided with primary shaft bearing 4, be provided with first rotation axis 5 in the primary shaft bearing 4, primary shaft bearing 4 set up make the rotation of first rotation axis 5 more smooth and easy, and mutual independence each other, the side of first rotation axis 5 is all fixed and is provided with fixed station 6.
The fixing table 6 is provided with a placing hole 601, when in actual use, the test tube 51 is placed in the placing hole 601, the placing hole 601 is provided with the air bag 7, the air bag 7 is communicated with the inflation adjusting device, the inflation degree of the air bag 7 can be adjusted through the inflation adjusting device, so that the test tubes 51 with different thickness specifications can be stably clamped, the universality is stronger, the first rotating shaft 5 is sleeved with the incomplete gear shaft 14 and the first limit nut 15 through threads, the incomplete gear shaft 14 can be meshed with the transmission gear 36, and the incomplete gear shaft 14 has a certain length, so that even after the incomplete gear shaft 14 rotates and moves along the first rotating shaft 5, the incomplete gear shaft 14 can still be meshed with the transmission gear 36, the number of the first limit nuts 15 in each group is two, and the first limit nuts are respectively arranged on two sides of the incomplete gear shaft 14, namely, one first limit nut 15 is respectively arranged on two sides of each incomplete gear shaft 14, incomplete gear shaft 14 is threaded setting on first rotation axis 5, after the position is adjusted in the rotation, can all rotate the first stop nut 15 of both sides to hug closely incomplete gear shaft 14, from this, when transmission gear 36 meshing incomplete gear shaft 14, incomplete gear shaft 14 can not take place to move about, and only can be driven rotatoryly, finally makes first rotation axis 5 take place to rotate, and then drives fixed station 6 and test tube 51 wherein and take place to vibrate the rotation.
A rack 16 is movably arranged in the main box body 1 and the auxiliary box body 2 in a penetrating way, a push plate 17 is fixedly arranged at the end part of the rack 16 positioned in the auxiliary box body 2, a compression spring 18 is connected between the push plate 17 and the outer wall of the main box body 1, the compression spring 18 has good telescopic elasticity, when the push plate 17 loses the external force to push, the compression spring 18 can push the push plate 17 back to the initial position under the action of the stretching restoring force, the push plate 17 can be intermittently pushed by the driving device, further, the rack 16 drives the driving gear 29 to intermittently rotate to and fro to a certain degree, one end of the rack 16 positioned in the main box body 1 is movably inserted in the groove of the concave block 22, and connect between rack 16 and the concave type piece 22 recess diapire and be provided with connecting spring 23, connecting spring 23's main effect is ejecting the recovery when losing the driving force with rack 16, and then makes rack 16 can take place reciprocating motion.
A guide rail 24 is fixedly arranged inside the main box body 1, a sliding plate 25 is movably arranged in the guide rail 24, the sliding plate 25 can move along the inner wall of the guide rail 24, a return spring 26 is connected between the sliding plate 25 and the inner wall of the main box body 1, a second bearing block 27 is embedded in one side of the sliding plate 25 away from the return spring 26, a second rotating shaft 28 is arranged in the second bearing block 27, the second rotating shaft 28 can smoothly rotate due to the arrangement of the second bearing block 27, a driving gear 29 is fixedly sleeved on the second rotating shaft 28, when the rack 16 moves, the driving gear 29 can be driven to rotate, a driving bevel gear 30 and a second limiting nut 31 are sleeved on the second rotating shaft 28 in a threaded manner, two second limiting nuts 31 in each group are respectively arranged on two sides of the driving bevel gear 30, and the driving bevel gear 30 can rotate and move along the second rotating shaft 28, and then the position thereof is adjusted, after the adjustment is completed, the second limit nuts 31 at both sides are rotated to be closely adhered to the drive bevel gear 30, and at this time, the drive bevel gear 30 is inevitably moved if rotated, but the second limit nuts 31 at both sides are limited, therefore, the driving bevel gear 30 is locked, the positioning block 3 is fixedly provided with the mounting rod 32, the side surface of the mounting rod 32 is embedded with the third bearing seat 33, the third bearing seat 33 is provided with the central rod 34, the central rod 34 can rotate in the third bearing seat 33, the central rod 34 is fixedly sleeved with the driven bevel gear 35 and the transmission gear 36, the driving bevel gear 30 can be meshed to drive the driven bevel gear 35 to rotate, the transmission gear 36 can be meshed to drive the incomplete gear shaft 14 to rotate, and, depending on the position of the toothed portion of the incomplete gear shaft 14, the extent to which the incomplete gear shaft 14 can be rotated varies.
The bottom of the main box body 1 is fixedly provided with a bearing seat 39, a piston tube 41 is embedded in the bearing seat 39, a piston plate 42 is movably arranged in the piston tube 41, an extension rod 43 is fixedly arranged on the piston plate 42, a shock absorption plate 44 is fixedly arranged on the extension rod 43, the shock absorption plate 44 has certain flexibility, the test tube 51 is prevented from being damaged by hard collision after falling off from the fixed platform 6, a shock absorption spring 45 is connected and arranged at the bottom of a bearing groove formed by the shock absorption plate 44 and the bearing seat 39, the piston tube 41 is communicated with a hollow ring 46, the hollow ring 46 is communicated with a plurality of convex tubes 47, a movable plate 48 is movably arranged in each convex tube 47, the movable plate 48 can move up and down along the inner wall of each convex tube 47, media such as piston oil and piston liquid are filled between the piston plate 42 and the movable plate 48, a connecting rod 49 is fixedly arranged on the movable plate 48, and the connecting rod 49 is abutted against the sliding plate 25, therefore, when the connecting rod 49 is pushed up, it can push up the slide plate 25, so that the return spring 26 at this time is further compressed.
Example two:
the utility model provides a cell culture is with shaking mixing arrangement, includes the main tank body 1, and the fixed vice box 2 that is provided with in side of main tank body 1, the fixed a plurality of locating pieces 3 that are provided with on the inner chamber lateral wall of main tank body 1, and the equal embedded first bearing frame 4 that is provided with in one side of vice box 2 is kept away from to locating piece 3 is provided with first rotation axis 5 in the first bearing frame 4, and the side of first rotation axis 5 is all fixed station 6 that is provided with.
The fixing table 6 is provided with a placing hole 601, when in actual use, the test tube 51 is placed in the placing hole 601, the placing hole 601 is provided with the air bag 7, the air bag 7 is communicated with the inflation adjusting device, the inflation degree of the air bag 7 can be adjusted through the inflation adjusting device, so that the test tubes 51 with different thickness specifications are stably clamped, the first rotating shaft 5 is sleeved with the incomplete gear shaft 14 and the first limit nut 15 in a threaded manner, the incomplete gear shaft 14 can be meshed with the transmission gear 36, and the incomplete gear shaft 14 has a certain length, so that even after the incomplete gear shaft 14 rotates and moves along the first rotating shaft 5, the incomplete gear shaft 14 can still be meshed with the transmission gear 36, each group of the first limit nuts 15 has two parts and is respectively arranged at two sides of the incomplete gear shaft 14, namely, two sides of each incomplete gear shaft 14 are respectively provided with one first limit nut 15, incomplete gear shaft 14 is threaded setting on first rotation axis 5, after the position is adjusted in the rotation, can all rotate the first stop nut 15 of both sides to hug closely incomplete gear shaft 14, from this, when transmission gear 36 meshing incomplete gear shaft 14, incomplete gear shaft 14 can not take place to move about, and only can be driven rotatoryly, finally makes first rotation axis 5 take place to rotate, and then drives fixed station 6 and test tube 51 wherein and take place to vibrate the rotation.
The rack 16 is movably arranged in the main box body 1 and the auxiliary box body 2 in a penetrating mode, a push plate 17 is fixedly arranged at the end portion of the rack 16 inside the auxiliary box body 2, a compression spring 18 is connected and arranged between the push plate 17 and the outer wall of the main box body 1, the push plate 17 can be intermittently pushed by a driving device, and then the rack 16 drives a driving gear 29 to intermittently and repeatedly rotate to a certain degree, one end of the rack 16 inside the main box body 1 is movably inserted into a groove of the concave block 22, and a connecting spring 23 is connected and arranged between the rack 16 and the groove bottom wall of the concave block 22.
A guide rail 24 is fixedly arranged in the main box body 1, a sliding plate 25 is movably arranged in the guide rail 24, a return spring 26 is connected between the sliding plate 25 and the inner wall of the main box body 1, a second bearing seat 27 is embedded in one side of the sliding plate 25 away from the return spring 26, a second rotating shaft 28 is arranged in the second bearing seat 27, a driving gear 29 is fixedly sleeved on the second rotating shaft 28, when the rack 16 moves, the driving gear 29 can be driven to rotate, a driving bevel gear 30 and a second limit nut 31 are sleeved on the second rotating shaft 28 in a threaded manner, two second limit nuts 31 are arranged in each group and are respectively arranged on two sides of the driving bevel gear 30, a mounting rod 32 is fixedly arranged on the positioning block 3, a third bearing seat 33 is embedded in the side surface of the mounting rod 32, a central rod 34 is arranged in the third bearing seat 33, and the central rod 34 can rotate in the third bearing seat 33, the central rod 34 is fixedly sleeved with a driven bevel gear 35 and a transmission gear 36, the driving bevel gear 30 can be meshed with and drive the driven bevel gear 35 to rotate, the transmission gear 36 can be meshed with and drive the incomplete gear shaft 14 to rotate, the amplitude of rotation of the incomplete gear shaft 14 can be changed according to different positions of a toothed part of the incomplete gear shaft 14, a third rotating shaft 37 is fixedly arranged on one side, away from the auxiliary box body 2, of the fixed table 6 at the non-head end, the third rotating shaft 37 is arranged in a fourth bearing seat 38, the fourth bearing seat 38 is embedded in the positioning block 3, the third rotating shaft 37 plays a role of assisting in supporting the fixed table 6, and each fixed table 6 can independently rotate and swing.
The bottom of the main box body 1 is fixedly provided with a bearing seat 39, a bearing groove of the bearing seat 39 is internally provided with an elastic cushion 40, the elastic cushion 40 has certain flexibility and can avoid the problem that the bearing seat 39 is directly contacted with a test tube 51 to be broken, a piston tube 41 is embedded in the bearing seat 39, a piston plate 42 is movably arranged in the piston tube 41, an extension rod 43 is fixedly arranged on the piston plate 42, a damping plate 44 is fixedly arranged on the extension rod 43, the damping plate 44 has certain flexibility and can avoid the test tube 51 from being rigidly collided and damaged after being separated from a fixed table 6 and falling, a damping spring 45 is connected and arranged at the bottom of the bearing groove formed by the damping plate 44 and the bearing seat 39, the piston tube 41 is communicated with a hollow ring 46, the hollow ring 46 is communicated with a plurality of projecting tubes 47, a movable plate 48 is movably arranged in the projecting tubes 47, the movable plate 48 can move up and down along the inner walls of the projecting tubes 47, the piston oil, the piston liquid and other media are filled between the piston plate 42 and the movable plate 48, the movable plate 48 is fixedly provided with a connecting rod 49, the connecting rod 49 is abutted against the sliding plate 25, therefore, when the connecting rod 49 is ejected upwards, the sliding plate 25 can be pushed upwards, the restoring spring 26 at the moment is further compressed, the inner wall of the opening end of the protruding tube 47 on the side far away from the hollow ring 46 is fixedly provided with a blocking block 50, the blocking block 50 is arranged to prevent the movable plate 48 from separating from the protruding tube 47 in the moving process, and further the device can stably operate.
Example three:
in the present embodiment, based on the first and second embodiments, the specific structure of the inflation regulating device is further disclosed and defined, specifically: the inflation adjusting device comprises an adjusting air pipe 8, the adjusting air pipe 8 is embedded in a fixing table 6, the adjusting air pipe 8 is communicated with an air bag 7, an adjusting plate 9 is movably arranged in the adjusting air pipe 8, air is stored in the adjusting air pipe 8 between the adjusting plate 9 and the air bag 7, the adjusting plate 9 can rotate and move in the adjusting air pipe 8, then the air in the adjusting air pipe 8 is pushed into the air bag 7, or the air in the air bag 7 is pumped back to the adjusting air pipe 8, so that the expansion degree of the air bag 7 is changed, test tubes 51 with different thickness specifications can be stably clamped, universality is stronger, a screw rod 10 is fixedly arranged on the adjusting plate 9, a plugging plate 11 is fixedly arranged inside one end, far away from the air bag 7, of the adjusting air pipe 8, a threaded hole 12 is formed in the plugging plate 11, threads of the screw rod 10 are arranged in the threaded hole 12, the screw rod 10 can rotate and move in the threaded hole 12, and then change its position, and the fixed handle 13 that is provided with of one end that keeps away from regulating plate 9 of screw rod 10, when in actual use, accessible handle 13 conveniently drives screw rod 10 and takes place the rotation, and it is more convenient to operate.
Example four:
in the present embodiment, based on the first and second embodiments, specific structures of the driving device are further disclosed and defined, specifically: the driving device comprises a rotating motor 19, the rotating motor 19 is arranged in the auxiliary box body 2, a transmission shaft 20 is fixedly arranged on an output shaft of the rotating motor 19, a cam 21 is fixedly arranged on the transmission shaft 20, the rotating motor 19 can drive the cam 21 to rotate through the transmission shaft 20 after being started, and the cam 21 can intermittently push the push plate 17 when continuously rotating, so that the rack 16 moves in a reciprocating manner, and finally the driving gear 29 is driven to rotate in a reciprocating manner.
The working principle is as follows:
when using, at first place test tube 51 in the hole 601 of placing of fixed station 6, then, handheld handle 13 and rotatory, handle 13 at this moment can drive regulating plate 9 through screw rod 10 and take place rotatoryly, screw rod 10 can be rotatory in the screw hole 12 of closure plate 11, make regulating plate 9 adjust the inside rotation of trachea 8 and shift position, at this moment, according to the removal of regulating plate 9, can push into gasbag 7 with the gas in regulating trachea 8, or take out the gas in gasbag 7 to regulating trachea 8, change the inflation degree of gasbag 7 from this, make test tube 51 of different thickness specifications can both be by the centre gripping steadily, the universality is stronger.
Before starting oscillation, if a certain test tube or some test tubes 51 do not need to oscillate or have already oscillated and do not need to oscillate again, at this time, the test tubes 51 do not need to be taken out for additional placement, but the driving bevel gear 30 and the two second limit nuts 31 at corresponding positions are rotated upwards, so that the driving bevel gear 30 is not meshed with the driven bevel gear 35 any more, and the driven bevel gear 35 cannot rotate at this time, and the fixing table 6 and the test tubes 51 therein cannot be driven to oscillate through the incomplete gear shaft 14 and the first rotating shaft 5; for other test tubes 51 which need to be oscillated and mixed, the driving device is started, so that the rotating motor 19 drives the cam 21 to rotate through the transmission shaft 20, the cam 21 can intermittently push the push plate 17 when continuously rotating, and further the rack 16 moves in a reciprocating manner, at this time, the driving gear 29 meshed with the rack 16 rotates, and further the second rotating shaft 28 rotates in situ, the driving bevel gear 30 on the second rotating shaft 28 drives the driven bevel gear 35 to rotate, so that the central rod 34 and the transmission gear 36 thereon rotate, and finally the transmission gear 36 drives the first rotating shaft 5 to rotate with a certain program through the incomplete gear shaft 14, so that the corresponding fixed table 6 and the test tube 51 arranged therein swing with a certain angle, and when the cam 21 is far away from the push plate 17, the push plate 17 is pushed back by the compression spring 18 to recover, the rack 16 at this time drives the driving gear 29 to move reversely, which finally causes the test tube 51 to swing reversely, so that the test tube 51 can swing reciprocally along with the reciprocating motion of the push plate 17, thereby shaking and mixing the cell culture solution in the test tube 51.
In the oscillation mixing operation process, generally, the larger the oscillation amplitude is, the better the mixing effect is, but the cell culture fluids in different test tubes 51 may be different, for individual test tubes 51 with more culture fluids, because the liquid level is high, if the test tubes 51 with low liquid level are oscillated with large amplitude in a swinging manner, the culture fluids are very easy to throw out, causing pollution, at this time, the position of the incomplete gear shaft 14 can be rotationally adjusted, so that the number of teeth which can be continuously meshed with the transmission gear 36 for transmission is reduced, and further the oscillation amplitude of the incomplete gear shaft 14 is reduced, and the incomplete gear shaft 14 has a certain length, therefore, even after the incomplete gear shaft 14 rotates along the first rotating shaft 5, the incomplete gear shaft can still be meshed with the transmission gear 36, after the uniform starting, other test tubes with low liquid level can still oscillate with large amplitude, so that the overall oscillation mixing effect is better, and the adaptability is strong.
When a test tube 51 is not clamped stably or is loosened after clamping and if no oscillation operation is found to be started, the fixing table 6 is very easy to break the test tube 51 during oscillation and oscillation, which is a safety hazard, in the present apparatus, the test tube 51 without clamping stability is not swung before oscillation is started, and therefore is in a vertical state, so that when falling off because of no clamping stability, the test tube 51 is vertically dropped into the bearing seat 39, the test tube 51 presses the damping plate 44 downward, and in order to ensure sufficient downward pressure, the test tube 51 should have a certain self-weight, for example, the test tube 51 may be a thickened glass test tube having a weight of at least 200g, and the firmness of the test tube 51 is improved, and in accordance therewith, the damping spring 45 pressed down by the test tube 51 should be selected to have a smaller coefficient, that is the smaller coefficient indicates that the damping spring 45 needs to perform a deformation amount per unit length, the damping spring 45 has a stiffness coefficient of, for example, 100N/m, which is given above only as an example, without limitation, the test tube 51 has enough weight to deform the damping spring 45 after being filled with the cell culture solution, and has enough weight to allow the shock absorbing plate 44 to press down the piston plate 42 through the extension rod 43, under the action of the piston tube 41, the piston fluid enters the hollow ring 46 and finally pushes out the movable plate 48 in the protruding tube 47, the movable plate 48 pushes the sliding plate 25 upwards through the connecting rod 49, the sliding plate 25 finally drives the driving gear 29 through the second rotating shaft 28, so that the driving gear 29 slides upwards to be separated from the rack 16, and the corresponding fixed platform 6 can not oscillate and rotate, therefore, the test tube 51 in the separated state can be protected, the functions are diversified, and the overall using effect is better.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. The utility model provides a cell culture is with shaking mixing arrangement, includes the main tank body (1), its characterized in that: an auxiliary box body (2) is fixedly arranged on the side face of the main box body (1), a plurality of positioning blocks (3) are fixedly arranged on the side wall of an inner cavity of the main box body (1), first bearing seats (4) are embedded in one sides, far away from the auxiliary box body (2), of the positioning blocks (3), first rotating shafts (5) are arranged in the first bearing seats (4), and fixing tables (6) are fixedly arranged on the side faces of the first rotating shafts (5);
a placing hole (601) is formed in the fixed table (6), an air bag (7) is arranged in the placing hole (601), the air bag (7) is communicated with an inflation adjusting device, an incomplete gear shaft (14) and first limiting nuts (15) are sleeved on the first rotating shaft (5) in a threaded manner, and two first limiting nuts (15) are arranged on each group and are respectively arranged on two sides of the incomplete gear shaft (14);
a rack (16) is movably arranged in the main box body (1) and the auxiliary box body (2) in a penetrating manner, a push plate (17) is fixedly arranged at the end part of the rack (16) positioned in the auxiliary box body (2), a compression spring (18) is connected between the push plate (17) and the outer wall of the main box body (1), the push plate (17) can be intermittently pushed by a driving device, one end of the rack (16) positioned in the main box body (1) is movably inserted in a groove of the concave block (22), and a connecting spring (23) is connected between the rack (16) and the bottom wall of the groove of the concave block (22);
a guide rail (24) is fixedly arranged in the main box body (1), a sliding plate (25) is movably arranged in the guide rail (24), a return spring (26) is connected and arranged between the sliding plate (25) and the inner wall of the main box body (1), a second bearing seat (27) is embedded in one side, away from the return spring (26), of the sliding plate (25), a second rotating shaft (28) is arranged in the second bearing seat (27), a driving gear (29) is fixedly sleeved on the second rotating shaft (28), a driving bevel gear (30) and a second limiting nut (31) are sleeved on the second rotating shaft (28) in a threaded manner, two second limiting nuts (31) are arranged in each group and are respectively arranged on two sides of the driving bevel gear (30), a mounting rod (32) is fixedly arranged on the positioning block (3), and a third bearing seat shaft (33) is embedded in the side surface of the mounting rod (32), a central rod (34) is arranged in the third bearing seat (33), and a driven bevel gear (35) and a transmission gear (36) are fixedly sleeved on the central rod (34);
the fixed bearing seat (39) that is provided with in bottom of the main tank body (1), embedded piston pipe (41) that is provided with in bearing seat (39), the activity is provided with piston plate (42) in piston pipe (41), the fixed extension rod (43) that is provided with on piston plate (42), fixed damping plate (44) that is provided with on extension rod (43), the bearing tank bottom connection that damping plate (44) and bearing seat (39) were seted up is provided with damping spring (45), piston pipe (41) communicate in well core ring (46), the intercommunication is provided with a plurality of protruding pipes (47) on well core ring (46), the activity is provided with fly leaf (48) in protruding pipe (47), the fixed connecting rod (49) that is provided with on fly leaf (48), connecting rod (49) and slide (25) looks butt.
2. The shaking mixing device for cell culture according to claim 1, wherein: aerify adjusting device is including adjusting trachea (8), adjust the embedded setting in fixed station (6) of trachea (8), just it is linked together to adjust trachea (8) and gasbag (7), it is provided with regulating plate (9) to adjust the activity in trachea (8), the fixed screw rod (10) that is provided with on regulating plate (9), it is provided with closure plate (11) to adjust the inside fixed one end that gasbag (7) were kept away from in trachea (8), set up screw hole (12) in closure plate (11), screw rod (10) screw thread sets up in screw hole (12), just the fixed handle (13) that is provided with of one end that regulating plate (9) were kept away from in screw rod (10).
3. The shaking mixing device for cell culture according to claim 1, wherein: the driving device comprises a rotating motor (19), the rotating motor (19) is arranged in the auxiliary box body (2), a transmission shaft (20) is fixedly arranged on an output shaft of the rotating motor (19), a cam (21) is fixedly arranged on the transmission shaft (20), and the cam (21) can intermittently push the push plate (17) when continuously rotating.
4. The shaking mixing device for cell culture according to claim 1, wherein: the side, away from the auxiliary box body (2), of the fixing table (6) located at the non-head end is fixedly provided with a third rotating shaft (37), the third rotating shaft (37) is arranged in a fourth bearing seat (38), and the fourth bearing seat (38) is embedded in a positioning block (3).
5. The shaking mixing device for cell culture according to claim 1, wherein: an elastic pad (40) is arranged in the supporting groove of the supporting seat (39).
6. The shaking mixing device for cell culture according to claim 1, wherein: and a stop block (50) is fixedly arranged on the inner wall of the opening end of one side, far away from the hollow ring (46), of the bulge tube (47).
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Effective date of registration: 20230727 Address after: 650031 Floor 1, Building 1, No. 298, Xuefu Road, Wuhua District, Kunming City, Yunnan Province Patentee after: Yunnan Lianhua Medical Technology Co.,Ltd. Address before: 650000 No. 68, Wenchang Road, 121 Avenue, Kunming, Yunnan Patentee before: Kunming University of Science and Technology |