CN114813714A - Test paper analyzer and detection analysis method - Google Patents

Test paper analyzer and detection analysis method Download PDF

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CN114813714A
CN114813714A CN202210267275.6A CN202210267275A CN114813714A CN 114813714 A CN114813714 A CN 114813714A CN 202210267275 A CN202210267275 A CN 202210267275A CN 114813714 A CN114813714 A CN 114813714A
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test paper
color
pins
controller
test
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王奎子
郝代旭
王烁
赵青
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Jilin Tstcheck Biological Electronic Engineering Co ltd
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Jilin Tstcheck Biological Electronic Engineering Co ltd
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N21/78Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01Arrangements or apparatus for facilitating the optical investigation
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/251Colorimeters; Construction thereof
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/27Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N2021/7756Sensor type
    • G01N2021/7759Dipstick; Test strip

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Abstract

A test paper analyzer and a detection analysis method are provided, the analyzer comprises an instrument main body, a test paper objective table, a motor, a PET white film, a color acquisition board, a power module and a control unit; the test paper objective table is arranged on a sliding rail in the instrument main body, a test paper groove is processed on the upper surface of the test paper objective table, a PET white film is pasted at a position close to the end part of the test paper groove, and a rack is fixedly arranged on one side of the test paper objective table opposite to the test paper groove; the motor is used for driving the rack; the color collecting plate is arranged above the test paper object stage, the color collecting plate is provided with a light source and a color collecting sensor, the control unit is used for calculating the actual RGB value of the test paper after reaction according to the RGB value collected by the color collecting sensor, and calculating the content of a certain chemical component or a certain specific class of chemical components or mineral ion components in the liquid to be detected according to the actual RGB value of the test paper. The analyzer solves the technical problems of large error, easy external interference and complex operation of visual colorimetric method visual water quality detection results.

Description

Test paper analyzer and detection analysis method
Technical Field
The invention belongs to the technical field of water quality analysis and detection, and particularly relates to a test paper analyzer and a detection and analysis method.
Background
The existing market has more equipment for water quality analysis, but the adopted analysis method mainly comprises an electrode method and a colorimetric method; wherein, the electrode method is mainly used for measuring TDS (total dissolved solids) in water; colorimetric methods are often used to determine the content of one or a particular class of chemical or mineral ionic components in water, such as total chlorine, residual chlorine, total alkalinity, total hardness, and the like.
Two types of colorimetric methods are commonly used: visual colorimetry and photoelectric colorimetry. The visual colorimetry is mainly observed by eyes, the object of the colorimetry can be a liquid reagent which completely reacts and can also be a test strip after the reaction is finished, and the method for testing the water quality by using the test strip is portable and visual, is less influenced by the temperature of the external environment, and is the most common detection method at present. However, when the color of the test strip is determined by using a visual observation method after reaction, a large error is easily generated, whether the test strip reaches the optimal reaction time is not easy to determine, and the operation difficulty is increased by outdoor operation. The photoelectric colorimeter mainly uses a photoelectric colorimeter for measurement, the photoelectric colorimeter eliminates subjective errors of a visual colorimeter and improves the measurement accuracy, but the photoelectric colorimeter adopts a tungsten lamp light source and an optical filter, is only suitable for a visible spectrum region and can only obtain composite light in a certain wavelength range, and has certain limitations. Therefore, it is necessary to develop a detection analyzer which can not only solve the problem of large error of the visual colorimetry, but also solve the problem of limited detection range of the photoelectric colorimetry.
Disclosure of Invention
The invention aims to provide a test paper analyzer which is provided with a test paper object stage and can calculate the content of one or a certain specific chemical component or mineral substance ion component in a water sample according to the RGB value of the test paper which reacts.
In order to achieve the purpose, the invention adopts the following technical scheme:
a test paper analyzer comprises an instrument main body, a test paper object stage, a motor, a PET white film, a color collecting plate, a power supply module and a control unit, wherein the test paper object stage, the motor, the PET white film, the color collecting plate, the power supply module and the control unit are arranged in the instrument main body; the test paper loading platform is arranged in the instrument main body along the longitudinal direction of the instrument main body, and slides along the slide rail, one end of the test paper loading platform penetrates through the instrument main body and extends to the outside, a test paper groove is formed in the upper surface of the test paper loading platform, a PET white film is adhered to the position close to the end part of the test paper groove and used for adjusting the white balance of a light source, and a rack is fixedly arranged on one side, opposite to the test paper groove, of the test paper loading platform; the output shaft of the motor is provided with a gear which is meshed with the rack and used for driving the rack; the color acquisition board is arranged above the test paper object stage, a light source and a color acquisition sensor are arranged on the color acquisition board, the light source is used for irradiating the test paper in the PET white film or the test paper groove, and the color acquisition sensor is used for acquiring the color of the test paper in the PET white film or the test paper groove and sending the acquired data to the control unit; the power supply module is used for supplying power; the control unit is used for calculating the actual RGB value of the reacted test paper according to the RGB value collected by the color collecting sensor, and calculating the content of one or a certain specific chemical component or mineral ion component in the liquid to be detected according to the actual RGB value of the test paper.
Preferably, the instrument main body is further provided with a buzzer and a Bluetooth module, wherein the buzzer is used for providing prompt tones and key tones for the instrument; the magnetite is still installed in the position that is close to the white film of PET to the test paper objective table, install hall sensor on the board is gathered to the colour, through the signal of hall sensor monitoring magnetite, realizes the location to the test paper objective table, makes the test paper objective table all reset to fixed initial position when testing at every turn.
Preferably, the instrument main body is provided with a display screen installation part and an operation key installation part, the display screen installation part is provided with a display screen, the operation key installation part is provided with an operation key, and the side wall of the instrument main body is provided with a USB data interface and a program downloading interface; the magnetite is cylindrical, the motor is step motor.
Preferably, the light source is an ultra-high brightness white light LED light source, and the display screen is an LCD display screen and is used for displaying the test result; the operation keys comprise a soft on-off key, a reset key, a lower key, an upper key, a determination key and a return key.
Preferably, the control unit comprises a controller, a memory; wherein pins 36, 37, 38, 39 and 40 of the controller are connected with pins 1, 16, 15, 10 and 9 of the motor driving module, pins 2, 4, 5 and 7 of the motor driving module are connected with the motor, pins 14, 16, 17, 29, 30, 55 and 56 of the controller are connected with the power supply module, pins 20, 34, 41, 44 and 45 of the controller are connected with the light source white balance module, the light source white balance module is used for adjusting the light source to the set brightness before the detection is started, the light source white balance module is connected with the LED light source on the color collecting board through the color collecting interface, pins 50, 51, 52 and 53 of the controller are connected with the color collecting sensor on the color collecting board through the color collecting interface, pins 21, 22, 23, 24, 25 and 35 of the controller are connected with an LCD display screen, pins 15 of the controller are connected with a buzzer, pins 46 and 49 of the controller are connected with a program downloading interface, pins 57 and 62 of the controller are connected with a soft on-off module, and the soft on-off module is connected with an operation key; pins 16 and 17 of the controller are connected with a USB data interface, and pins 29, 30, 55 and 56 of the controller are connected with a Bluetooth module; the 7 pins of the controller are connected with the reset key, the 58 pins and 59 pins of the controller are connected with the lower key and the upper key, and the 8 pins and 9 pins of the controller are connected with the confirm key and the return key.
Preferably, the memory is used for storing data.
Another object of the present invention is to provide a method for detecting and analyzing a test strip analyzer, which comprises the following steps:
step S1, press the soft key start-up of opening and shutting down, start the test paper analysis appearance, the motor action, the test paper objective table begins the initialization, makes PET white film under the color acquisition sensor, and the rgb value of the white film of color acquisition sensor collection PET is marked as: wr, Wg, Wb;
step S2, the control unit calculates a scale factor of white balance according to the rgb value of the PET white film, and the calculation formula is as follows:
Krgb=Wr/255 Kr=1.00000 Kg=Wr/Wg Kb=Wr/Wb
step S3, when the water sample to be tested needs to be tested, pressing the menu key, moving the cursor to select the 'selection item', and adjusting the test type to the item to be tested; when a plurality of components in a water sample are detected simultaneously, all items to be detected can be selected simultaneously;
s4, placing test paper matched with the analyzer in a solution to be tested for several seconds, wherein the waiting time of different test paper is different, determining the waiting time according to the selected trial production, and placing the test paper on a test paper object stage of a test paper analyzer after the test paper is fully reflected; the test paper contains 1 to 6 test paper blocks according to the requirements of test items, and when a single item needs to be detected, the test paper only contains a single test paper block; when a plurality of items need to be detected simultaneously, the number of the test paper blocks is set according to the number of the detected items; each test paper block contains a substance which has color change reaction with different detection substances; if the test paper contains a plurality of test paper blocks, the test paper blocks are arranged at intervals along the length direction of the test paper, and the position relation between the detection substance contained in each test paper block and the test paper blocks is fixed;
s5, after the test paper is placed in a test paper groove of a test paper object stage, the test paper is pushed inwards until the test paper is pushed to the head, then a motor rotates at a constant speed to drive the test paper object stage to move at a constant speed, the test paper moves at a constant speed, the test paper passes through a color acquisition sensor at a constant speed, a controller receives data sent by the color acquisition sensor, the controller obtains the central position of a first test paper block through data processing and analysis according to the data acquisition time and the motor rotating speed, and then the central positions of second to Nth test paper blocks are determined according to the intervals among the test paper blocks, so that the RGB original value of the central position of each test paper block is obtained;
step S6, calculating the actual RGB value of the test paper by the controller according to the RGB original value of each test paper block in the test paper collected by the color collecting sensor and the white balance scale factor obtained in the step S2; the calculation formula is as follows:
Tr=TR*Kr/Krgb
Tg=TG*Kg/Krgb
Tb=TB*Kb/Krgb
the RGB original values of each test paper block in the test paper collected by the color collecting sensor are TR, TG and TB, and the actual RGB values of the test paper blocks are Tr, Tg and Tb;
and step S7, comparing and judging the R value or the G value or the B value of the single channel which can most indicate the color change in the obtained actual rgb values of each test paper block in the test paper with the sensitivity threshold value stored in the memory, thereby determining the detection concentrations of different detection items in the liquid to be detected, and displaying the concentration of the liquid to be detected tested by the current test paper on the display screen.
Preferably, the correspondence between the standard solutions with different concentrations stored in the memory and the RGB values and the sensitivity threshold are obtained as follows:
step S1, respectively configuring at least 7 standard solutions with the concentrations of C1, C2, C3, C4, C5, C6, C7 and Cn for each detection item, wherein the color of the standard solutions with different concentrations is gradually changed into a standard color gradation after the standard solutions with different concentrations react with the medicament on the detection test paper; and the standard solution contains the lowest concentration and the highest concentration of the detected substance;
step S2, starting the test paper analyzer, the motor acts, the test paper objective table starts initialization, the white PET film is under the color acquisition sensor, the rgb value of the white PET film acquired by the color acquisition sensor is recorded as: wr, Wg, Wb;
step S3, the control unit calculates a scale factor of white balance according to the rgb value of the PET white film, and the calculation formula is as follows:
Krgb=Wr/255 Kr=1.00000 Kg=Wr/Wg Kb=Wr/Wb
step S4, placing the test paper containing a single test paper block into a test tube filled with a standard solution with a certain concentration, completely immersing the test paper into the liquid to be tested, taking out the test paper after waiting for several seconds, throwing the test paper by hand, placing the test paper on a test paper objective table, enabling the test paper to advance at a constant speed under the color acquisition sensor by the action of a motor, and recording the rgb value of the test paper acquired by the color acquisition sensor as: TR, TG, TB; wherein, the test paper block contains a substance which has a color change reaction with a detection component in the liquid to be detected;
step S5, the control unit calculates the actual rgb value of the test paper according to the rgb value of the test paper collected by the color collecting sensor and the white balance scale factor, and records the calculated rgb value as: tr, Tg and Tb, and the calculation formula is as follows:
Tr=TR*Kr/Krgb
Tg=TG*Kg/Krgb
Tb=TB*Kb/Krgb
step S6, sequentially obtaining the corresponding relations between at least 7 standard solutions with different concentrations and RGB values according to the manner of steps 1 to 5, and then selecting a single channel R value or G value or B value that can most indicate color change from among the RGB values, taking reading the R values of the reaction of the 7 standard solutions with different concentrations and test paper as an example, establishing the corresponding relations between R1 and C1, between R2 and C2, between R3 and C3, between R4 and C4, between R5 and C5, between R6 and C6, and between R7 and C7;
step S7, the control unit reads the R channel values of the 7 standard solutions with different concentrations and gradually increasing concentrations: r1, R2, R3, R4, R5, R6, R7, respectively; the actual sensitivity setting parameters are: (R1+ R2)/2, (R2+ R3)/2, (R3+ R4)/2, (R4+ R5)/2, (R5+ R6)/2, (R6+ R7)/2; and (3) actually testing the liquid to be tested: the value is C1 when R actually > (R1+ R2)/2, C2 when (R2+ R3)/2< R actually ≦ (R1+ R2)/2, C3 when (R3+ R4)/2< R actually ≦ (R2+ R3)/2, C4 when (R4+ R5)/2< R actually ≦ (R3+ R4)/2, C5 when (R5+ R6)/2< R actually ≦ (R4+ R5)/2, C6 when (R6+ R7)/2< R actually ≦ (R5+ R6)/2, and C7 when R actually ≦ (R6+ R7)/2.
The invention has the advantages and beneficial effects that:
(1) the test paper analyzer provided by the invention has the advantages of small and simple structure, simple operation, convenient use, high detection speed and low cost, calculates the color of the test paper according to the reflected light component of the test paper obtained by the color acquisition sensor, calculates the content of a certain chemical component or a certain specific chemical component or mineral ion component in a water sample to be detected according to the color of the test paper, has accurate and reliable detection result, and solves the technical problems of large error, easy external interference, complex operation and the like when the water quality detection result is observed by naked eyes; and simultaneously, the problem of limitation of a photoelectric colorimetric method is also solved.
(2) The test paper analyzer provided by the invention is matched with corresponding analysis test paper, and can be used for quantitative detection of seven items of the swimming pool, six items of the aquatic animal, ammonia nitrogen (0-20mg/L), ammonia nitrogen (0-100mg/L), ammonia nitrogen (0-1000mg/L) and other items.
(3) The test paper analyzer provided by the invention adopts a quasi-32-bit singlechip, so that peripheral devices are reduced to the maximum extent, and the reliability of products is improved; the signal-to-noise ratio of the system is improved by adopting the latest ultrahigh-brightness white light LED light source; by adopting a high-performance photoelectric receiving device and an RGB (red, green and blue) three-component color measurement principle, the instrument has better anti-interference capability and adaptability.
(4) The test paper analyzer provided by the invention can store up to 100 test results for a user, is managed according to batch numbers, and is convenient for the user to look up afterwards.
Drawings
FIG. 1 is a front view of a strip analyzer of the present invention;
FIG. 2 is a side view of the strip analyzer of the present invention;
FIG. 3 is a rear view of the strip analyzer of the present invention;
FIG. 4 is a top view of the strip analyzer of the present invention;
FIG. 5 is a schematic view showing the internal structure of the strip analyzer of the present invention;
FIG. 6 is a schematic block diagram of the connection of the components of the strip analyzer of the present invention;
FIG. 7 is a circuit diagram of a controller;
FIG. 8 is a circuit diagram of the power module of the present invention;
FIG. 9 is a circuit diagram of the soft switching power module of the present invention;
FIG. 10 is a circuit diagram of an LCD display screen according to the present invention;
FIG. 11 is a circuit diagram of a color collection plate of the present invention;
FIG. 12 is a circuit diagram of the color acquisition interface of the present invention;
FIG. 13 is a circuit diagram of the motor drive module of the present invention;
FIG. 14 is a circuit diagram of a light source white balance module of the present invention;
FIG. 15 is a circuit diagram of a USB data interface of the present invention;
FIG. 16 is a circuit diagram of a Bluetooth module of the present invention;
fig. 17 is a circuit diagram of the buzzer of the present invention.
Reference numerals: the device comprises a test paper A, an instrument main body 1, a test paper objective table 2, a motor 3, a color acquisition board 4, a power module 5, a control unit 6, a buzzer 7, a Bluetooth module 8, a display screen 11, an operation key 12, a slide rail 13, a USB data interface 14, a rack 21, a light source 41 and a color acquisition sensor 42.
Detailed Description
The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all of the embodiments.
In the description of the present application, it should be noted that the terms "in", "under", and the like indicate the orientation or positional relationship: the particular arrangements or components shown in the drawings, or the orientations or positional relationships conventionally used in the manufacture of the applications, are for convenience only and to simplify the description, and are not intended to indicate or imply that the referenced devices or components must be in a particular orientation, constructed and operated in a particular orientation, and are not to be construed as limiting the application. Furthermore, the terms "first," "second," and the like are used merely to distinguish one description from another, and are not to be construed as indicating or implying relative importance.
In the description of the present application, it is also to be noted that, unless otherwise explicitly specified or limited, the terms "disposed" and "connected" are to be interpreted broadly, e.g., as being either fixedly connected, detachably connected, or integrally connected; they may be mechanically coupled, directly coupled, indirectly coupled through intervening media, or interconnected between two elements. The specific meaning of the above terms in the present application can be understood in a specific case by those of ordinary skill in the art.
Example 1
Referring to fig. 1 to 6, the test paper analyzer provided by the present invention includes an apparatus main body 1, a test paper stage 2 installed inside the apparatus main body, a motor 3, a PET white film, a color collecting plate 4, a power module 5, and a control unit 6; the instrument main body 1 is provided with a display screen mounting part and an operation key mounting part, a display screen 11 is mounted on the display screen mounting part, and the display screen 11 adopts an LCD display screen and is used for displaying a test result; an operation key 12 is installed on the operation key installation part, a slide rail 13 is arranged in the instrument main body 1 along the longitudinal direction of the instrument main body, the test paper object stage 2 is installed on the slide rail and slides along the slide rail, one end of the test paper object stage 2 penetrates through the instrument main body 1 and extends to the outside, a test paper groove is processed on the upper surface of the test paper object stage 2, a PET white film is adhered to the position close to the end part of the test paper groove, and a rack 21 is fixedly installed on one side of the test paper object stage opposite to the test paper groove; the output shaft of the motor 3 is provided with a gear which is meshed with the rack 21 and is used for driving the rack; the color acquisition board 4 is arranged above the test paper object stage 2, the color acquisition board 4 is provided with a light source 41 and a color acquisition sensor 42, the light source 41 is used for irradiating the test paper A in the PET white film or the test paper groove, the light source adopts an ultra-high brightness white light LED light source, and the color acquisition sensor 42 is used for acquiring the color of the test paper A in the PET white film or the test paper groove and sending the acquired data to the control unit; the power supply module 5 is used for supplying power; the control unit 6 is used for calculating the actual RGB value of the test paper after reaction according to the RGB value collected by the color collecting sensor, and calculating the content of a certain chemical component or a certain specific chemical component or mineral ion component in the liquid to be detected according to the actual RGB value of the test paper.
Further, the instrument main body 1 of this embodiment is further provided with a buzzer 7 and a bluetooth module 8, and the buzzer 7 is used for providing prompt tones and key tones for the instrument; the test paper objective table is also provided with a magnet at a position close to the PET white film, the color acquisition board is provided with a Hall sensor, and the Hall sensor is used for monitoring the signal of the magnet to realize the positioning of the test paper objective table, so that the test paper objective table is reset to a fixed initial position during each test; a USB data interface 14 and a program downloading interface are arranged on the side wall of the instrument main body 1; the operation keys comprise a soft on-off key, a menu key, a downward key, an upward key and a confirmation key.
Further, the magnetite is cylindrical, the motor is step motor.
Referring to fig. 7 to 17, the control unit includes a controller, a memory; wherein pins 36, 37, 38, 39 and 40 of the controller are connected with pins 1, 16, 15, 10 and 9 of the motor driving module, pins 2, 4, 5 and 7 of the motor driving module are connected with the motor, pins 14, 16, 17, 29, 30, 55 and 56 of the controller are connected with the power supply module, pins 20, 34, 41, 44 and 45 of the controller are connected with the light source white balance module, the light source white balance module is used for adjusting the light source to the set brightness before the detection is started, the light source white balance module is connected with the LED light source on the color collecting board through the color collecting interface, pins 50, 51, 52 and 53 of the controller are connected with the color collecting sensor on the color collecting board through the color collecting interface, pins 21, 22, 23, 24, 25 and 35 of the controller are connected with an LCD display screen, pins 15 of the controller are connected with a buzzer, pins 46 and 49 of the controller are connected with a program downloading interface, pins 57 and 62 of the controller are connected with a soft on-off module, the soft on-off module is connected with an operation key, pins 16 and 17 of the controller are connected with a USB data interface, and pins 29, 30, 55 and 56 of the controller are connected with a Bluetooth module; pins 7, 8, 9, 58 and 59 of the controller are connected with the operation keys.
In this embodiment, the memory stores RGB values of the standard solutions and the reactive test strips at different concentrations for each detection item, and a sensitivity threshold value for comparison in actual testing, which is calculated according to the RGB values of the standard solutions, and the controller calculates the actual RGB values of the reactive test strips according to the data of the color acquisition sensor, and then calculates the content of a specific chemical component or a specific class of chemical components or mineral ion components in the water sample according to the sensitivity threshold value in the memory and the corresponding relationship between the standard solutions and the RGB values at different concentrations; the corresponding relation between the standard solutions with different concentrations and the RGB values and the acquisition mode of the sensitivity threshold are as follows:
step S1, respectively configuring at least 7 standard solutions with the concentrations of C1, C2, C3, C4, C5, C6, C7 and Cn for each detection item, wherein the color of the standard solutions with different concentrations is gradually changed into a standard color gradation after the standard solutions with different concentrations react with the medicament on the detection test paper; and the standard solution contains the lowest concentration and the highest concentration of the detected substance;
step S2, starting the test paper analyzer, enabling the motor to act, initializing the test paper objective table, enabling the PET white film to be right below the color acquisition sensor, and recording the rgb value of the PET white film acquired by the color acquisition sensor as: wr, Wg, Wb;
step S3, the control unit calculates a scale factor of white balance according to the rgb value of the PET white film, and the calculation formula is as follows:
Krgb=Wr/255 Kr=1.00000 Kg=Wr/Wg Kb=Wr/Wb
step S4, placing the test paper containing a single test paper block into a test tube filled with a standard solution with a certain concentration, completely immersing the test paper into the liquid to be detected, taking out the test paper (corresponding to the detection item of ammonia nitrogen of 0-20 mg) after waiting for five seconds, throwing the test paper with hands, placing the test paper on a test paper objective table, enabling the test paper to advance at a constant speed under a color acquisition sensor by the action of a motor, and recording the rgb value of the test paper acquired by the color acquisition sensor as: TR, TG, TB; wherein, the test paper block contains a substance which has a color change reaction with a detection component in the liquid to be detected;
step S5, the control unit calculates the actual rgb value of the test paper according to the rgb value of the test paper collected by the color collecting sensor and the white balance scale factor, and records the calculated rgb value as: tr, Tg and Tb, and the calculation formula is as follows:
Tr=TR*Kr/Krgb
Tg=TG*Kg/Krgb
Tb=TB*Kb/Krgb
step S6, sequentially obtaining the corresponding relations between at least 7 standard solutions with different concentrations and RGB values according to the manner of steps 1 to 5, and then selecting a single channel R value or G value or B value that can most indicate color change from among the RGB values, taking reading the R values of the reaction of the 7 standard solutions with different concentrations and test paper as an example, establishing the corresponding relations between R1 and C1, between R2 and C2, between R3 and C3, between R4 and C4, between R5 and C5, between R6 and C6, and between R7 and C7;
step S7, the control unit reads the R channel values of the 7 standard solutions with different concentrations and gradually increasing concentrations: r1, R2, R3, R4, R5, R6, R7, respectively; the actual sensitivity setting parameters are: (R1+ R2)/2, (R2+ R3)/2, (R3+ R4)/2, (R4+ R5)/2, (R5+ R6)/2, (R6+ R7)/2; and (3) actually testing the liquid to be tested: the value is C1 when R actually > (R1+ R2)/2, C2 when (R2+ R3)/2< R actually ≦ (R1+ R2)/2, C3 when (R3+ R4)/2< R actually ≦ (R2+ R3)/2, C4 when (R4+ R5)/2< R actually ≦ (R3+ R4)/2, C5 when (R5+ R6)/2< R actually ≦ (R4+ R5)/2, C6 when (R6+ R7)/2< R actually ≦ (R5+ R6)/2, and C7 when R actually ≦ (R6+ R7)/2.
The detection principle of the invention is as follows:
after the test paper is put into a water sample to be detected, the medicine on the test paper reacts with a substance to be detected in the water sample, so that the test paper can display different colors. Treat in the water sample that the material concentration is different, the colour is also different. After the test strip is irradiated by incident light in the instrument and reflected, light with specific wavelength can be absorbed, the absorption degree is related to the color of the test strip, and the RGB value of the test strip is determined according to the absorption degree. For example: the color of the test paper block of the ammonia nitrogen detection test paper is yellow to green, and the deeper the color, the higher the analysis result.
Example 2
In order to make the test strip analyzer provided in the present invention clear to those skilled in the art, a method for detecting and analyzing a test strip analyzer is described in detail below, the method comprising the steps of:
step S1, pressing the soft on-off button to start up, starting the test paper analyzer, the motor acts, the test paper objective table starts initialization, so that the PET white film is under the color acquisition sensor, the rgb value of the PET white film acquired by the color acquisition sensor is recorded as: wr, Wg, Wb;
step S2, the control unit calculates a scale factor of white balance according to the rgb value of the PET white film, and the calculation formula is as follows:
Krgb=Wr/255 Kr=1.00000 Kg=Wr/Wg Kb=Wr/Wb
step S3, when the water sample to be tested needs to be tested, pressing the menu key, moving the cursor to select the 'selection item', and adjusting the test type to the item to be tested; when a plurality of components in a water sample are detected simultaneously, all items to be detected can be selected simultaneously, and 6 items can be selected at most under the limitation of the length of the test paper and the structure of an instrument;
s4, placing the test paper matched with the instrument in a solution to be tested for several seconds, wherein the waiting time of different test papers is different, determining the waiting time according to the selected test system, and placing the test paper on a test paper objective table of a test paper analyzer after the test paper is fully reflected; the test paper contains 1 to 6 test paper blocks according to the requirements of test items, and when a single item needs to be detected, the test paper only contains a single test paper block; when a plurality of items need to be detected simultaneously, the number of the test paper blocks is set according to the number of the detected items; each test paper block contains a substance which has color change reaction with different detection substances; if the test paper contains a plurality of test paper blocks, the test paper blocks are arranged at intervals along the length direction of the test paper, and the position relationship between the detection substance contained in each test paper block and the test paper blocks is fixed, so that subsequent data processing is facilitated;
step S5, placing the test paper into a test paper groove of a test paper object stage, pushing the test paper inwards until the test paper is pushed to the head, then driving the test paper object stage to move at a constant speed by rotating a motor at a constant speed, enabling the test paper to move at a constant speed, enabling the test paper to pass through a color acquisition sensor at a constant speed, receiving 600 groups of data sent by the color acquisition sensor by a controller, processing and analyzing the data by the controller according to the time of data acquisition, the rotating speed of the motor and the like to obtain the central position of a first test paper block, and determining the central positions of second to Nth test paper blocks according to the intervals among the test paper blocks so as to obtain the RGB original values of the central positions of all the test paper blocks;
step S6, calculating the actual RGB value of the test paper by the controller according to the RGB original value of each test paper block in the test paper collected by the color collection sensor and the white balance scale factor obtained in step S2; the calculation formula is as follows:
Tr=TR*Kr/Krgb
Tg=TG*Kg/Krgb
Tb=TB*Kb/Krgb
the RGB original values of each test paper block in the test paper collected by the color collecting sensor are TR, TG and TB, and the actual RGB values of the test paper blocks are Tr, Tg and Tb;
and step S7, comparing and judging the R value or the G value or the B value of the single channel which can most indicate the color change in the obtained actual rgb values of each test paper block in the test paper with the sensitivity threshold value stored in the memory, thereby determining the detection concentrations of different detection items in the liquid to be detected, and displaying the concentration of the liquid to be detected tested by the current test paper on the display screen.
The test paper analyzer provided by the invention is matched with corresponding analysis test paper, and can be used for quantitative detection of six items of the swimming pool, ammonia nitrogen (0-20mg/L), ammonia nitrogen (0-100mg/L), ammonia nitrogen (0-1000mg/L) and the like.
The foregoing is considered as illustrative of the preferred embodiments of the invention and is not to be construed as limiting the invention in any way. Various equivalent changes and modifications can be made by those skilled in the art based on the above embodiments, and all equivalent changes and modifications within the scope of the claims should fall within the protection scope of the present invention.

Claims (8)

1. A test paper analyzer is characterized by comprising an instrument main body, a test paper object stage, a motor, a PET white film, a color acquisition board, a power module and a control unit, wherein the test paper object stage, the motor, the PET white film, the color acquisition board, the power module and the control unit are arranged in the instrument main body; the test paper loading platform is arranged in the instrument main body along the longitudinal direction of the instrument main body, and slides along the slide rail, one end of the test paper loading platform penetrates through the instrument main body and extends to the outside, a test paper groove is formed in the upper surface of the test paper loading platform, a PET white film is adhered to the position close to the end part of the test paper groove and used for adjusting the white balance of a light source, and a rack is fixedly arranged on one side, opposite to the test paper groove, of the test paper loading platform; the output shaft of the motor is provided with a gear which is meshed with the rack and used for driving the rack; the color acquisition board is arranged above the test paper object stage, a light source and a color acquisition sensor are arranged on the color acquisition board, the light source is used for irradiating the test paper in the PET white film or the test paper groove, and the color acquisition sensor is used for acquiring the color of the test paper in the PET white film or the test paper groove and sending the acquired data to the control unit; the power supply module is used for supplying power; the control unit is used for calculating the actual RGB value of the reacted test paper according to the RGB value collected by the color collecting sensor, and calculating the content of one or a certain specific chemical component or mineral ion component in the liquid to be detected according to the actual RGB value of the test paper.
2. A test paper analyzer according to claim 1, wherein the main body of the analyzer is further provided with a buzzer and a bluetooth module, the buzzer is used for providing prompt tones and key tones for the analyzer; the magnetite is still installed in the position that is close to the white film of PET to the test paper objective table, install hall sensor on the board is gathered to the colour, through the signal of hall sensor monitoring magnetite, realizes the location to the test paper objective table, makes the test paper objective table all reset to fixed initial position when testing at every turn.
3. A test paper analyzer according to claim 2, wherein the analyzer body is provided with a display screen mounting portion on which a display screen is mounted and an operation key mounting portion on which an operation key is mounted; a USB data interface and a program downloading interface are arranged on the side wall of the instrument main body; the magnetite is cylindrical, the motor is step motor.
4. A test paper analyzer according to claim 2, wherein the light source is an ultra-high brightness white LED light source, and the display screen is an LCD display screen for displaying the test result; the operation keys comprise a soft on-off key, a reset key, a lower key, an upper key, a determination key and a return key.
5. A strip analyzer according to claim 4, wherein the control unit includes a controller, a memory; wherein pins 36, 37, 38, 39 and 40 of the controller are connected with pins 1, 16, 15, 10 and 9 of the motor driving module, pins 2, 4, 5 and 7 of the motor driving module are connected with the motor, pins 14, 16, 17, 29, 30, 55 and 56 of the controller are connected with the power supply module, pins 20, 34, 41, 44 and 45 of the controller are connected with the light source white balance module, the light source white balance module is used for adjusting the light source to the set brightness before the detection is started, the light source white balance module is connected with the LED light source on the color collecting board through the color collecting interface, pins 50, 51, 52 and 53 of the controller are connected with the color collecting sensor on the color collecting board through the color collecting interface, pins 21, 22, 23, 24, 25 and 35 of the controller are connected with an LCD display screen, pins 15 of the controller are connected with a buzzer, pins 46 and 49 of the controller are connected with a program downloading interface, pins 57 and 62 of the controller are connected with a soft on-off module, and the soft on-off module is connected with an operation key; pins 16 and 17 of the controller are connected with a USB data interface, and pins 29, 30, 55 and 56 of the controller are connected with a Bluetooth module; the 7 pins of the controller are connected with the reset key, the 58 pins and 59 pins of the controller are connected with the lower key and the upper key, and the 8 pins and 9 pins of the controller are connected with the confirm key and the return key.
6. A strip analyzer according to claim 5, wherein the memory is adapted to store data.
7. The method for detecting and analyzing a strip analyzer according to claim 1, comprising the steps of:
step S1, press the soft key start-up of opening and shutting down, start the test paper analysis appearance, the motor action, the test paper objective table begins the initialization, makes PET white film under the color acquisition sensor, and the rgb value of the white film of color acquisition sensor collection PET is marked as: wr, Wg, Wb;
step S2, the control unit calculates a scale factor of white balance according to the rgb value of the PET white film, and the calculation formula is as follows:
Krgb=Wr/255 Kr=1.00000 Kg=Wr/Wg Kb=Wr/Wb
step S3, when the water sample to be tested needs to be tested, pressing the menu key, moving the cursor to select the 'selection item', and adjusting the test type to the item to be tested; when a plurality of components in a water sample are detected simultaneously, all items to be detected can be selected simultaneously;
s4, placing test paper matched with the analyzer in a solution to be tested for several seconds, wherein the waiting time of different test paper is different, determining the waiting time according to the selected trial production, and placing the test paper on a test paper object stage of a test paper analyzer after the test paper is fully reflected; the test paper contains 1 to 6 test paper blocks according to the requirements of test items, and when a single item needs to be detected, the test paper only contains a single test paper block; when a plurality of items need to be detected simultaneously, the number of the test paper blocks is set according to the number of the detected items; each test paper block contains a substance which has color change reaction with different detection substances; if the test paper contains a plurality of test paper blocks, the test paper blocks are arranged at intervals along the length direction of the test paper, and the position relation between the detection substance contained in each test paper block and the test paper blocks is fixed;
s5, after the test paper is placed in a test paper groove of a test paper object stage, the test paper is pushed inwards until the test paper is pushed to the head, then a motor rotates at a constant speed to drive the test paper object stage to move at a constant speed, the test paper moves at a constant speed, the test paper passes through a color acquisition sensor at a constant speed, a controller receives data sent by the color acquisition sensor, the controller obtains the central position of a first test paper block through data processing and analysis according to the data acquisition time and the motor rotating speed, and then the central positions of second to Nth test paper blocks are determined according to the intervals among the test paper blocks, so that the RGB original value of the central position of each test paper block is obtained;
step S6, calculating the actual RGB value of the test paper by the controller according to the RGB original value of each test paper block in the test paper collected by the color collection sensor and the white balance scale factor obtained in step S2; the calculation formula is as follows:
Tr=TR*Kr/Krgb
Tg=TG*Kg/Krgb
Tb=TB*Kb/Krgb
the RGB original values of each test paper block in the test paper collected by the color collecting sensor are TR, TG and TB, and the actual RGB values of the test paper blocks are Tr, Tg and Tb;
and step S7, comparing and judging the R value or the G value or the B value of the single channel which can most indicate the color change in the obtained actual rgb values of each test paper block in the test paper with the sensitivity threshold value stored in the memory, thereby determining the detection concentrations of different detection items in the liquid to be detected, and displaying the concentration of the liquid to be detected tested by the current test paper on the display screen.
8. The test strip analyzer detection and analysis method of claim 7, wherein the correspondence between the standard solutions of different concentrations stored in the memory and the RGB values and the sensitivity threshold are obtained in the following manner:
step S1, respectively configuring at least 7 standard solutions with the concentrations of C1, C2, C3, C4, C5, C6, C7 and Cn for each detection item, wherein the color of the standard solutions with different concentrations is gradually changed into a standard color gradation after the standard solutions with different concentrations react with the medicament on the detection test paper; and the standard solution contains the lowest concentration and the highest concentration of the detected substance;
step S2, starting the test paper analyzer, the motor acts, the test paper objective table starts initialization, the white PET film is under the color acquisition sensor, the rgb value of the white PET film acquired by the color acquisition sensor is recorded as: wr, Wg, Wb;
step S3, the control unit calculates a scale factor of white balance according to the rgb value of the PET white film, and the calculation formula is as follows:
Krgb=Wr/255Kr=1.00000Kg=Wr/Wg Kb=Wr/Wb
step S4, placing the test paper containing a single test paper block into a test tube filled with a standard solution with a certain concentration, completely immersing the test paper into the liquid to be tested, taking out the test paper after waiting for several seconds, throwing the test paper by hand, placing the test paper on a test paper objective table, enabling the test paper to advance at a constant speed under the color acquisition sensor by the action of a motor, and recording the rgb value of the test paper acquired by the color acquisition sensor as: TR, TG, TB; wherein, the test paper block contains a substance which has a color change reaction with a detection component in the liquid to be detected;
step S5, the control unit calculates the actual rgb value of the test paper according to the rgb value of the test paper collected by the color collecting sensor and the white balance scale factor, and records the calculated rgb value as: tr, Tg and Tb, and the calculation formula is as follows:
Tr=TR*Kr/Krgb
Tg=TG*Kg/Krgb
Tb=TB*Kb/Krgb
step S6, sequentially obtaining the corresponding relations between at least 7 standard solutions with different concentrations and RGB values according to the manner of steps 1 to 5, then selecting a single channel R value or G value or B value that can most indicate color change from among the RGB values, and if the R values of the reaction of the 7 standard solutions with different concentrations and test paper are read, establishing the corresponding relations between R1 and C1, between R2 and C2, between R3 and C3, between R4 and C4, between R5 and C5, between R6 and C6, and between R7 and C7;
step S7, the control unit reads the R channel values of the 7 standard solutions with different concentrations and gradually increasing concentrations: r1, R2, R3, R4, R5, R6, R7, respectively; the actual sensitivity setting parameters are: (R1+ R2)/2, (R2+ R3)/2, (R3+ R4)/2, (R4+ R5)/2, (R5+ R6)/2, (R6+ R7)/2; and (3) actually testing the liquid to be tested: the value is C1 when R actually > (R1+ R2)/2, C2 when (R2+ R3)/2< R actually ≦ (R1+ R2)/2, C3 when (R3+ R4)/2< R actually ≦ (R2+ R3)/2, C4 when (R4+ R5)/2< R actually ≦ (R3+ R4)/2, C5 when (R5+ R6)/2< R actually ≦ (R4+ R5)/2, C6 when (R6+ R7)/2< R actually ≦ (R5+ R6)/2, and C7 when R actually ≦ (R6+ R7)/2.
CN202210267275.6A 2022-03-18 2022-03-18 Test paper analyzer and detection analysis method Pending CN114813714A (en)

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