CN114813612A - Multi-channel single plant photosynthetic rate measuring system and method - Google Patents

Multi-channel single plant photosynthetic rate measuring system and method Download PDF

Info

Publication number
CN114813612A
CN114813612A CN202210481919.1A CN202210481919A CN114813612A CN 114813612 A CN114813612 A CN 114813612A CN 202210481919 A CN202210481919 A CN 202210481919A CN 114813612 A CN114813612 A CN 114813612A
Authority
CN
China
Prior art keywords
gas
assimilation
boxes
sampling
gas circuit
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202210481919.1A
Other languages
Chinese (zh)
Inventor
熊栋梁
郑林娜
凌霄霞
王真梅
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Huazhong Agricultural University
Original Assignee
Huazhong Agricultural University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Huazhong Agricultural University filed Critical Huazhong Agricultural University
Priority to CN202210481919.1A priority Critical patent/CN114813612A/en
Publication of CN114813612A publication Critical patent/CN114813612A/en
Pending legal-status Critical Current

Links

Images

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
    • G01N21/35Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
    • G01N21/3504Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light for analysing gases, e.g. multi-gas analysis
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00584Control arrangements for automatic analysers

Landscapes

  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

The invention relates to a multichannel single plant photosynthetic rate measuring system, a multichannel single plant photosynthetic rate measuring method and a storage medium, wherein the measuring system comprises a gas flux measuring device and a signal acquisition device. The gas flux measuring device comprises a non-diffusion infrared gas detector, a gas path controller, a sampling gas path and at least two assimilation boxes, and the signal acquisition device comprises a data acquisition unit. The non-diffusion infrared gas detector is used for detecting the gas concentration entering and leaving the sampling gas circuit of at least two assimilation boxes; the data acquisition unit is used for outputting a control signal to the gas circuit controller at a first preset time interval so that the gas circuit controller controls the communication state of the at least two assimilation boxes and the sampling gas circuit; the data collector is also used for recording the gas concentration signal at a second preset time interval and determining the gas flux of the individual plant by using the relationship between the gas concentration signal and the gas flow rate. The measuring system can automatically measure the photosynthetic rate of the single plant in multiple channels.

Description

Multi-channel single plant photosynthetic rate measuring system and method
Technical Field
The invention relates to the technical field of plant physiology research equipment, in particular to a multichannel single plant photosynthesis rate measuring system and method.
Background
Photosynthesis is the most important chemical reaction on earth, and the energy converted by the process is the basis on which earth life depends to live. At present, the photosynthesis rate is mainly based on photosynthetic oxygen evolution and CO 2 Gas and three major approaches based on optical properties, among others, based on CO 2 The method for measuring the gas has the advantages of no damage in situ, high accuracy and the like, and is a measuring mode with wider application.
Based on CO 2 The gas measuring way has different measuring principles, and the method for measuring the chamber infrared gas is widely applied to the scales of blades, whole plants, canopies and the like. With the technological progress and intensive research on photosynthesis, techniques for measuring photosynthetic rate at the level of single leaf and canopy have been commercialized, but techniques for measuring photosynthetic rate at the level of single plant are not yet mature. Photosynthesis at the level of the individual plants serves as a bridge to communicate between single-leaf and canopy photosynthesis, and the lack of this partial determination technique results in the inability of photosynthesis at the level of the single leaf to establish a direct link with canopy photosynthesis. Therefore, how to establish a set of mature single plant photosynthetic assay system, solve the problems that the single plant photosynthetic assay device cannot realize multi-channel and automatic assay, has high operation cost and the like, and is a problem to be solved urgently.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention provides a multi-channel single plant photosynthetic rate measuring system and a multi-channel single plant photosynthetic rate measuring method, which can meet the requirement of multi-channel automatic measurement of the photosynthetic rate of a single plant and provide a new idea for reducing photosynthetic measurement cost.
In order to solve the above problems, in a first aspect, the present invention provides a multichannel single plant photosynthetic rate measuring system, including: a gas flux measuring device and a signal collecting device; the gas flux measuring device comprises a non-diffusion infrared gas detector, a gas path controller, a sampling gas path and at least two assimilation boxes; the signal acquisition device comprises a data acquisition unit.
Further, the non-diffusion infrared gas detector is used for detecting gas concentration signals of at least two assimilation boxes entering and leaving the sampling gas circuit; the data acquisition unit is used for outputting a control signal to the gas circuit controller at a first preset time interval so that the gas circuit controller controls the communication state of the at least two assimilation boxes and the sampling gas circuit; the data acquisition unit is further used for recording the gas concentration signal at a second preset time interval and determining the gas flux of the individual plant by using the relationship between the gas concentration signal and the gas flow rate.
Further, the gas flux measuring device further comprises an air pump and a gas flow meter. The air pump is used for supplying air to the at least two assimilation boxes; the gas flowmeter is used for detecting gas flow velocity signals in the at least two assimilation boxes.
Further, the gas circuit controller includes relay controller and two at least solenoid valves, relay controller is used for passing through opening and shutting of two at least solenoid valves is in order to control two at least assimilation casees with the connection status of sampling gas circuit.
Further, the assimilation box comprises an upper assimilation chamber and a lower pot placing chamber; the upper assimilation chamber comprises an air inlet pipeline, an air outlet pipeline and a fan; the fan is used for stirring the gas in the assimilation box.
Further, the air inlet pipeline is connected with the air pump through a gas flowmeter and used for pumping air into the assimilation box; the gas outlet pipeline is connected with the sampling gas circuit and used for outputting gas to the non-diffusion infrared gas detector;
further, the assimilation box also comprises a flange gasket with a hole and a sealing ring; the number of the flange gaskets is two, and the two flange gaskets are respectively used for connecting the upper assimilation chamber and the lower pot placing chamber; the sealing ring comprises two hollow semicircular acrylic plates so that the plant stem passes through the hollow position.
Further, the system also comprises an environmental factor sensor, specifically a temperature sensor, a pressure sensor and a photosynthetically active radiation sensor; the temperature sensor with pressure sensor installs assimilation incasement portion lateral wall, photosynthetic active radiation sensor installs the outside top of assimilation box.
Furthermore, the signal acquisition device also comprises an acquisition device expansion board which is used for acquiring temperature, pressure and photosynthetically active radiation data detected by the environmental factor sensor.
Further, the system also comprises a terminal device for recording and displaying the gas concentration signal, the gas flow rate signal and the gas flux of the individual plant in real time.
In a second aspect, the present invention also provides a method for determining the photosynthetic rate of a multichannel single plant, comprising:
detecting gas concentration signals of at least two assimilation boxes entering and leaving a sampling gas circuit through a non-diffusion infrared gas detector;
outputting a control signal to a gas circuit controller at a first preset time interval through a data acquisition unit so that the gas circuit controller controls the communication state of the at least two assimilation boxes and the sampling gas circuit;
and recording the gas concentration signal at a second preset time interval through the data acquisition unit, and determining the gas flux of the individual plant by using the relationship between the gas concentration signal and the gas flow rate.
The beneficial effects of adopting the above embodiment are:
in the invention, a part of gas fully and uniformly mixed in the assimilation box enters the non-diffusion infrared gas detector through the gas outlet hole at the top of the assimilation box and the sampling gas circuit, and CO in the assimilation box is detected by the non-diffusion infrared gas detector 2 And (5) detecting the concentration. The instrument measures CO 2 The concentration range is large, the resolution and the precision are high, and the accurate determination of the photosynthetic rate of the single plant can be realized. In addition, a control signal is output to the gas circuit controller through the data acquisition unit, so that the gas circuit controller controls the communication state of any assimilation box and a sampling gas circuit, the gas circuit controller is combined with a self-made plant assimilation box to form an open-circuit type measuring system, multi-channel and automatic measurement can be realized through a specific program, and manual intervention during operation is avoided. The invention has low operation cost and convenient use, and greatly improves the working efficiency.
Drawings
FIG. 1 is a schematic structural diagram of an embodiment of a multi-channel single plant photosynthetic rate measuring system provided by the present invention;
fig. 2 is a schematic structural diagram of a gas flux measuring apparatus according to an embodiment of the present invention;
fig. 3 is a schematic structural diagram of an assimilation box according to an embodiment of the present invention;
FIG. 4 is a schematic structural view of upper and lower seals of an assimilation box according to an embodiment of the present invention;
FIG. 5 is a flowchart of a method for determining a photosynthetic rate of a multichannel individual plant according to an embodiment of the present invention.
Detailed Description
The preferred embodiments of the present invention will now be described in detail with reference to the accompanying drawings, which form a part hereof, and which together with the embodiments of the invention serve to explain the principles of the invention and not to limit its scope.
In the description of the present invention, "a plurality" means two or more unless specifically defined otherwise. Reference herein to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment can be included in at least one embodiment of the invention. The appearances of the phrase in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments. It is explicitly and implicitly understood by one skilled in the art that the embodiments described herein can be combined with other embodiments.
The invention provides a multichannel single plant photosynthesis rate determination system, a multichannel single plant photosynthesis rate determination method and a storage medium, which are respectively explained below.
The invention discloses a multi-channel single plant photosynthetic rate measuring system, which comprises a gas flux measuring device and a signal acquisition device. The gas flux measuring device comprises a non-diffusion infrared gas detector, a gas path controller, a sampling gas path and at least two assimilation boxes; the signal acquisition device comprises a data acquisition unit.
The non-diffusion infrared gas detector is used for detecting gas concentration signals of at least two assimilation boxes entering and leaving the sampling gas circuit.
It should be noted that the infrared gas measuring mode of the chamber is widely applied to the level of the blade, the whole plant and the canopy, and the non-diffusion infrared gas detector in the embodiment of the invention has a strong detection function and mainly detects CO in the airflow of the assimilation box 2 The gas in the assimilation box can reach a gas detector through a sampling gas circuit, and the gas detector detects CO in the gas flow 2 Concentration, and storing. One end of the sampling gas circuit can be connected with the gas outlet holes at the upper parts of the assimilation boxes, and the other end of the sampling gas circuit is connected with the gas detector.
Wherein, the number of the assimilation boxes is at least two, and the CO of the whole plant in different assimilation boxes can be detected 2 And the concentration provides conditions for measuring the photosynthetic rate of the single plant through multiple channels.
The data acquisition unit is used for outputting a control signal to the gas circuit controller at a first preset time interval so that the gas circuit controller controls the communication state of the at least two assimilation boxes and the sampling gas circuit.
Specifically, the first preset time interval may be 3min, the data acquisition device may send a control signal to the controller every 3min, and the controller controls the communication state between the assimilation boxes and the sampling gas circuit after receiving the control signal, that is, the open time of each assimilation box is 3 min.
It can be understood that, because the number of the assimilation boxes is at least two, the multichannel gas circulation can be sequentially measured by controlling the communication state of the assimilation boxes and the sampling gas circuit. Furthermore, program-based timing design control provides conditions for achieving automated measurements.
The data collector is also used for recording the gas concentration signal at a second preset time interval and determining the gas flux of the individual plant by using the relationship between the gas concentration signal and the gas flow rate.
In the determination process of the photosynthetic rate of the individual plant, the gas detector is used for detecting and analyzing CO 2 After the gas concentration, CO is output 2 The gas concentration signal is sent to a signal acquisition device, specifically, output to a data acquisition deviceAccording to the collector. The data collector may record the CO at a second predetermined time interval 2 E.g. recording CO at time intervals of 0.1s to 3s 2 Data on gas concentration signals, and thus gas flux for individual plants, from sample and reference chamber gas concentrations and gas flow rates.
It is understood that the photosynthetic rate of an individual plant means that the photosynthetic rate of the individual plant can be CO for the individual plant placed in the assimilation box 2 When the CO of the individual plant is determined 2 The gas flux can also be regarded as determining the photosynthetic rate of the individual plant.
CO 2 Gas flux of Fco 2 Can use CO entering and leaving the tank body of the assimilation tank 2 Is expressed in units of. mu. mol. s -1 The calculation formula is as follows: fco 2 =C in *Flow in -C chamber *Flow chamber
Wherein, C in Indicating CO in the gas entering the tank 2 Mole fraction, Flow in Represents the molar flow rate of gas entering the tank, C chamber Indicating CO in the gas leaving the tank 2 Molarity, Flow chamber Representing the molar flow rate of the gas exiting the tank, the parameters in the above equation can all be determined by the present measurement system.
In the invention, a part of gas fully and uniformly mixed in the assimilation box enters the non-diffusion infrared gas detector through the gas outlet hole at the top of the assimilation box and the sampling gas circuit, and the CO2 concentration in the assimilation box is detected by using the non-diffusion infrared gas detector. The instrument has the advantages of wide range of CO2 concentration measurement, high resolution and high precision, and can realize accurate determination of the photosynthetic rate of a single plant. In addition, a control signal is output to the gas circuit controller through the data acquisition unit, so that the gas circuit controller controls the communication state of any assimilation box and a sampling gas circuit, the gas circuit controller is combined with a self-made plant assimilation box to form an open-circuit type measuring system, multi-channel and automatic measurement can be realized through a specific program, and manual intervention during operation is avoided. The invention has low operation cost and convenient use, and greatly improves the working efficiency.
For a more clear understanding of the present invention, please refer to fig. 1, fig. 1 is a schematic structural diagram of an embodiment of a multi-channel single plant photosynthetic rate measuring system provided by the present invention.
The multi-channel single plant photosynthesis rate measuring system comprises a gas flux measuring device, an environmental factor sensor, a signal acquisition device and a terminal (not shown in the figure). The gas flux measuring device comprises a non-diffusion infrared gas detector 3, a gas path controller (comprising a relay controller 4 and an electromagnetic valve 5), a gas flowmeter (not shown in the figure), a sampling gas path and at least two assimilation boxes; the environmental factor sensor comprises a temperature sensor, a pressure sensor and a photosynthetically active radiation sensor; the data acquisition device comprises a data acquisition unit 1 and an acquisition unit expansion board 2; the terminal is a terminal capable of recording and visualizing the data, and the software on the terminal can display and record the measured data in real time.
The air pump 6 is used for pumping air into at least two assimilation boxes, as shown in fig. 1, the air pumped by the air pump 6 enters from an air inlet at the lower part of the assimilation box and is output to a sampling air circuit from an air outlet at the upper part of the assimilation box, air flow enters the gas detector through the sampling air circuit, and the sampling air circuit is respectively communicated with the air outlet at the upper part of each assimilation box.
The gas circuit controller includes relay controller 4 and two at least solenoid valves 5, and relay controller 4 is used for opening and shutting through two at least solenoid valves 5 in order to control the connected state of two at least assimilation boxes and sampling gas circuit, and is concrete, and relay controller 4 realizes the break-make of sampling gas circuit in proper order through the switch of controlling solenoid valve 5 to realize multichannel's continuation survey.
In an embodiment of the present invention, before the gas flow enters the gas detector through the sampling gas path, the gas flow is monitored by a gas flow meter, and specifically, the gas flow meter is used for detecting gas flow rate signals in at least two assimilation boxes.
The environment factor sensor comprises a temperature sensor, a pressure sensor and a photosynthetically active radiation sensor, the temperature sensor and the pressure sensor are arranged on the side wall of the interior of the assimilation box, and the photosynthetically active radiation sensor is arranged at the top end of the exterior of the assimilation box; specifically, the temperature sensor is used for monitoring the temperature inside the assimilation box in real time; the pressure sensor is used for monitoring the pressure in the assimilation box in real time; the photosynthetically active radiation sensor is used to monitor photosynthetically active radiation impinging on the surface of the assimilation tank.
The data collector 1 of the signal acquisition part is connected with an acquisition expansion board 2, a relay controller 4 and a gas detector, and the acquisition expansion board 2 acquires temperature, pressure and photosynthetic effective radiation data detected by an environmental factor sensor in each assimilation box and transmits the data to the data collector 1; the data acquisition unit 1 can record and store data in real time, and specifically, the data acquisition unit can record the gas concentration and the data of other sensor signals at a time interval of 1 s;
the data collector 1 is also connected to the terminal, and the code for controlling the system is stored in the terminal, so that the automatic control and multi-channel measurement of the sampling gas circuit switching can be realized on the software of the terminal, and the automatic recording and data storage can be completed. In addition, the terminal also records and displays data such as gas concentration signals, gas flow rate signals, sensing signals in each assimilation box, gas flux of individual plants and the like in real time.
Referring to fig. 2, fig. 2 is a schematic structural diagram of a gas flux measuring apparatus according to an embodiment of the present invention.
The gas flux measuring device comprises an air pump 6, an assimilation box 18, a sampling gas circuit 19, an electromagnetic valve 5, a relay controller 4, a gas flowmeter 21 and a (non-diffused infrared) gas detector 3.
The air pump 6 is respectively connected with each assimilation box 18 through a gas flowmeter 21 and is used for pumping air, and the flow rate of the air pump is 7.2L per minute; the gas flow meter 21 is used for detecting a gas flow velocity signal in each assimilation box; wherein, the assimilation box is made of high-hardness acrylic plates with the light transmittance of 90% -95%, the specification of the assimilation box is 65cm by 20cm, and the number of the assimilation boxes is preferably 12-16; the relay controller 4 is respectively connected with the air inlet of each assimilation box 18 to control the assimilation box to be closed or opened, specifically, the data acquisition unit 1 outputs control signals to the relay controller 4 at regular time, and the relay controller 4 drives the potential of the electromagnetic valve 5 to change through electric signals so as to control the sampling air inlet sequence of the assimilation boxes 18; the gas flowing through the air outlet of the assimilation box 18 is divided into a main path and a branch path, the branch path enters the air, and the main path enters a sampling gas path 19; the gas entering and exiting the assimilation box 18 is conveyed by a sampling gas circuit, the sampling gas circuit 19 comprises a gas inlet pipeline and a gas outlet pipeline, the gas inlet pipeline is installed at the lower part of the assimilation box 18, the gas outlet pipeline is installed at the upper part of the assimilation box 18, the pipeline is a hose (PE material) 1-3 m long, and the inner diameter of the hose is 4mm, and the outer diameter of the hose is 6 mm; the gas flow is then measured by the flow meter 21 and then enters the (non-diffused infrared) gas detector 3 for gas concentration measurement.
Referring to fig. 3, fig. 3 is a schematic structural diagram of an assimilation box according to an embodiment of the present invention.
Wherein, the assimilation box comprises an upper assimilation chamber 7 and a lower pot placing chamber 8, and the upper assimilation chamber 7 comprises an air inlet pipeline 11, an air outlet pipeline 10 and a (mixed air) fan 9; the air inlet pipeline 11 is connected with an air pump through an air flow meter and used for inputting air to the assimilation box; the gas outlet pipeline 10 is connected with the sampling gas circuit and is used for outputting gas to the non-diffusion infrared gas detector; the (gas mixing) fan 9 is positioned at the bottom of the upper assimilation chamber 7 and used for rapidly mixing gas in the assimilation box, and specifically, 2 fans which are vertically arranged relatively are arranged at the bottom of the upper assimilation chamber 7, the size of each fan is 8cm x 8cm, the rotating speed is 2500rpm, and 5v power is supplied; the photosynthetically active radiation sensor 12 is positioned at the upper part of the box body and is used for measuring photosynthetically active radiation irradiated to the surface of the box body; the pressure sensor 13 and the temperature sensor 14 are positioned inside the box body, and the change of the pressure and the temperature inside the box body can be monitored in real time.
It should be noted that the assimilation box in this embodiment is an open-circuit assimilation box, and the internal environment changes little, and this technique is simple in structure and easy to operate.
Referring to fig. 4, fig. 4 is a schematic structural diagram of upper and lower seals of an assimilation box according to an embodiment of the present invention.
Wherein, an upper flange gasket 15, a lower flange gasket 16 and a sealing ring 17 are arranged between the upper assimilation chamber 7 and the lower pot placing chamber 8 of the assimilation box; the flange washers 15 and 16 are used for connecting the upper assimilation chamber and the lower pot placing chamber, wherein the flange holes are fixed by butterfly-shaped hand-screwed screws and nuts; the sealing ring 17 is used for sealing the assimilation box, and the sealing ring 17 is a hollow sealing ring, specifically two hollow semicircular acrylic plates, so that the plant stem can penetrate through the hollow position. And a foam sealing ring is used for sealing during measurement so as to achieve the sealing performance of the upper part and the lower part of the assimilation box.
The embodiment of the present invention further provides a method for determining a photosynthetic rate of a multichannel individual plant, please refer to fig. 5, where fig. 5 is a flowchart of a method according to an embodiment of the method for determining a photosynthetic rate of a multichannel individual plant provided by the present invention, and the method includes:
step S501: detecting gas concentration signals of at least two assimilation boxes entering and leaving a sampling gas circuit through a non-diffusion infrared gas detector;
step S502: outputting a control signal to the gas circuit controller at a first preset time interval through the data acquisition unit so that the gas circuit controller controls the communication state of at least two assimilation boxes and the sampling gas circuit;
step S503: and recording the gas concentration signal at a second preset time interval through the data acquisition unit, and determining the gas flux of the individual plant by using the relationship between the gas concentration signal and the gas flow rate.
Here, it should be noted that: the method for determining the photosynthetic rate of the multichannel single plant provided by the embodiment can realize the technical scheme described in the embodiment of the system for determining the photosynthetic rate of the multichannel single plant, and details are not repeated here.
Those skilled in the art will appreciate that all or part of the flow of the method implementing the above embodiments may be implemented by a computer program, which is stored in a computer readable storage medium, to instruct related hardware. The computer readable storage medium is a magnetic disk, an optical disk, a read-only memory or a random access memory.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are included in the scope of the present invention.

Claims (8)

1. A multi-channel single plant photosynthetic rate measuring system is characterized in that: comprises a gas flux measuring device and a signal acquisition device; the gas flux measuring device comprises a non-diffusion infrared gas detector, a gas path controller, a sampling gas path and at least two assimilation boxes; the signal acquisition device comprises a data acquisition unit;
the non-diffusion infrared gas detector is used for detecting gas concentration signals of at least two assimilation boxes entering and leaving the sampling gas circuit;
the data acquisition unit is used for outputting a control signal to the gas circuit controller at a first preset time interval so that the gas circuit controller controls the communication state of the at least two assimilation boxes and the sampling gas circuit;
the data acquisition unit is further used for recording the gas concentration signal at a second preset time interval and determining the gas flux of the individual plant by using the relationship between the gas concentration signal and the gas flow rate.
2. The system of claim 1, wherein: the gas flux measuring device also comprises an air pump and a gas flowmeter;
the air pump is used for pumping air into the at least two assimilation boxes;
the gas flow meter is used for detecting gas flow rate signals in the at least two assimilation boxes;
the gas circuit controller comprises a relay controller and at least two electromagnetic valves, wherein the relay controller is used for controlling the opening and closing of the at least two electromagnetic valves so as to control the connection state of the at least two assimilation boxes and the sampling gas circuit.
3. The system of claim 1, wherein: the assimilation box comprises an upper assimilation chamber and a lower pot placing chamber, wherein the upper assimilation chamber comprises an air inlet pipeline, an air outlet pipeline and a fan;
the air inlet pipeline is connected with the air pump through a gas flowmeter and used for pumping air into the assimilation box;
the gas outlet pipeline is connected with the sampling gas circuit and used for outputting gas to the non-diffusion infrared gas detector;
the fan is used for stirring the gas in the assimilation box.
4. The system of claim 3, wherein: the assimilation box also comprises a flange gasket with a hole and a sealing ring;
the number of the flange gaskets is two, and the two flange gaskets are respectively used for connecting the upper assimilation chamber and the lower pot placing chamber;
the sealing ring comprises two hollow semicircular acrylic plates so that the plant stem passes through the hollow position.
5. The system of claim 1, wherein: the system also comprises an environmental factor sensor, wherein the environmental factor sensor comprises a temperature sensor, a pressure sensor and a photosynthetically active radiation sensor;
the temperature sensor and the pressure sensor are installed on the side wall of the inner side of the assimilation box, and the photosynthetically active radiation sensor is installed on the top end of the outer portion of the assimilation box.
6. The system of claim 1, wherein: the signal acquisition device further comprises an acquisition device expansion plate, and the acquisition device expansion plate is used for acquiring temperature, pressure and photosynthetic active radiation signals detected by the environmental factor sensor.
7. The system of claim 1, wherein: the system also includes a terminal device; and the terminal equipment is used for recording and displaying the gas concentration signal, the gas flow velocity signal and the gas flux of the single plant in real time.
8. A multichannel single plant photosynthesis rate measurement method according to any one of claims 1 to 7, comprising:
detecting the gas concentrations of at least two assimilation boxes entering and leaving a sampling gas circuit through a non-diffusion infrared gas detector;
outputting a control signal to the gas circuit controller at a first preset time interval through a data acquisition unit so that the gas circuit controller controls the communication state of the at least two assimilation boxes and the sampling gas circuit;
and recording the gas concentration signal at a second preset time interval through the data acquisition unit, and determining the gas flux of the individual plant by using the relationship between the gas concentration signal and the gas flow rate.
CN202210481919.1A 2022-05-05 2022-05-05 Multi-channel single plant photosynthetic rate measuring system and method Pending CN114813612A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202210481919.1A CN114813612A (en) 2022-05-05 2022-05-05 Multi-channel single plant photosynthetic rate measuring system and method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202210481919.1A CN114813612A (en) 2022-05-05 2022-05-05 Multi-channel single plant photosynthetic rate measuring system and method

Publications (1)

Publication Number Publication Date
CN114813612A true CN114813612A (en) 2022-07-29

Family

ID=82510934

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202210481919.1A Pending CN114813612A (en) 2022-05-05 2022-05-05 Multi-channel single plant photosynthetic rate measuring system and method

Country Status (1)

Country Link
CN (1) CN114813612A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117741055A (en) * 2023-11-30 2024-03-22 华中农业大学 Greenhouse gas emission monitoring device based on ESP32
CN118566444A (en) * 2024-08-01 2024-08-30 北京复兰环保科技有限公司 Photosynthetic rate detection apparatus and method

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117741055A (en) * 2023-11-30 2024-03-22 华中农业大学 Greenhouse gas emission monitoring device based on ESP32
CN118566444A (en) * 2024-08-01 2024-08-30 北京复兰环保科技有限公司 Photosynthetic rate detection apparatus and method

Similar Documents

Publication Publication Date Title
CN114813612A (en) Multi-channel single plant photosynthetic rate measuring system and method
US9982225B2 (en) Integrated bio-reactor monitor and control system
CN108663347A (en) Optical dissolved oxygen sensor multi-parameter interference compensation corrects system and method
US20050087027A1 (en) A configurable multi-point sampling method and system for representative gas composition measurements in a stratified gas flow stream
ES2906617T3 (en) Monitoring of deviations from a state in bioreactors
CN109855705A (en) A kind of the oil flowmeter calibrating installation and calibration method of real-time dynamic compensation temperature variation
CN207882233U (en) A kind of dynamic case and Flux measuring system
CN113063902A (en) Portable analysis system for field real-time determination of plant photosynthesis
CN109085316A (en) Measure the device of water body dissolved methane concentration
CN109338017A (en) PH value automatic testing method and device in haematococcus pluvialis incubation
CN109061214A (en) Porous sampling device
CN204008587U (en) The rising measuring instrument of a kind of canopy photosynthesis
McFarlane et al. Plant exposure chambers for study of toxic chemical‐plant interactions
CN116694465A (en) Cell culture device capable of monitoring cell culture liquid environment
CN207636473U (en) Natural gas water content rate detection device and natural gas gas recovery facility set group
CN105067505A (en) Automatic concentration control sulfur dioxide test box
CN113917091A (en) Aquaculture waste compost ammonia emission sampling device
CN213336325U (en) Gas yield detection equipment in fermentation test
CN217331793U (en) Intelligent smoke discharge continuous monitoring device
CN212433997U (en) Underground storage operation simulation experiment equipment
KR200168089Y1 (en) A reaction gas analysis ststem for fermentation tube
CN219434634U (en) Experimental device for simulating concrete corrosion of non-full-flow sewage pipeline
CN218099095U (en) Detection device for detecting environment occurrence
CN221078574U (en) Automatic titration flow path
CN216594979U (en) Portable analysis system for field real-time determination of plant photosynthesis

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination