CN114810761A - Fluorescent microscopic imaging system and installation method and bonding structure thereof - Google Patents
Fluorescent microscopic imaging system and installation method and bonding structure thereof Download PDFInfo
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- CN114810761A CN114810761A CN202110325855.1A CN202110325855A CN114810761A CN 114810761 A CN114810761 A CN 114810761A CN 202110325855 A CN202110325855 A CN 202110325855A CN 114810761 A CN114810761 A CN 114810761A
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- 238000000034 method Methods 0.000 title claims abstract description 35
- 238000003384 imaging method Methods 0.000 title claims description 28
- 238000009434 installation Methods 0.000 title abstract description 11
- 239000000853 adhesive Substances 0.000 claims abstract description 49
- 230000001070 adhesive effect Effects 0.000 claims abstract description 49
- 238000000799 fluorescence microscopy Methods 0.000 claims abstract description 47
- 239000003292 glue Substances 0.000 claims abstract description 24
- 238000004026 adhesive bonding Methods 0.000 claims abstract description 18
- 238000005286 illumination Methods 0.000 claims description 18
- 239000012790 adhesive layer Substances 0.000 claims description 16
- 238000010008 shearing Methods 0.000 claims description 3
- 230000007774 longterm Effects 0.000 abstract description 5
- 239000010410 layer Substances 0.000 description 14
- 230000008602 contraction Effects 0.000 description 8
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- 235000017166 Bambusa arundinacea Nutrition 0.000 description 6
- 235000017491 Bambusa tulda Nutrition 0.000 description 6
- 241001330002 Bambuseae Species 0.000 description 6
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 6
- 239000011425 bamboo Substances 0.000 description 6
- 238000001514 detection method Methods 0.000 description 6
- 230000000694 effects Effects 0.000 description 4
- 239000000084 colloidal system Substances 0.000 description 3
- 230000005693 optoelectronics Effects 0.000 description 3
- 238000013459 approach Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000006073 displacement reaction Methods 0.000 description 2
- 239000000428 dust Substances 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 238000010030 laminating Methods 0.000 description 2
- 238000012423 maintenance Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000000018 DNA microarray Methods 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000002929 anti-fatigue Effects 0.000 description 1
- 230000000712 assembly Effects 0.000 description 1
- 238000000429 assembly Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
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- 230000002349 favourable effect Effects 0.000 description 1
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
- F16B—DEVICES FOR FASTENING OR SECURING CONSTRUCTIONAL ELEMENTS OR MACHINE PARTS TOGETHER, e.g. NAILS, BOLTS, CIRCLIPS, CLAMPS, CLIPS OR WEDGES; JOINTS OR JOINTING
- F16B11/00—Connecting constructional elements or machine parts by sticking or pressing them together, e.g. cold pressure welding
- F16B11/006—Connecting constructional elements or machine parts by sticking or pressing them together, e.g. cold pressure welding by gluing
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0052—Optical details of the image generation
- G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
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- Physics & Mathematics (AREA)
- Mechanical Engineering (AREA)
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- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Mounting And Adjusting Of Optical Elements (AREA)
Abstract
The invention discloses a fluorescence microscopic imaging system, an installation method thereof and an adhesion structure. The bonding structure comprises a first component and a second component which are fixed by gluing; the adhesive surfaces of the first component and the second component comprise a plurality of tenon-and-mortise splicing surfaces which are mutually matched and nested; any pair of tenon-and-mortise splicing surfaces which are mutually glued and at least one pair of tenon-and-mortise splicing surfaces which are mutually glued are distributed oppositely and are not vertical. The first component and the second component of the bonding structure are connected in an occlusion manner to form a plurality of pairs of tenon-and-mortise splicing surfaces positioned at the joint, and any pair of tenon-and-mortise splicing surfaces which are mutually attached are fixed in an adhesive manner. The multiple pairs of mortise and tenon joint surfaces are distributed oppositely and are not perpendicular, so that the stress and the volume change generated by the glue layers between the multiple pairs of mortise and tenon joint surfaces are offset and compensated, the position drift of the first component and the second component is greatly weakened or even avoided, and the long-term position precision of the product with the first component and the second component is ensured.
Description
Technical Field
The invention relates to the field of tools, in particular to a bonding structure. Still relate to a fluorescence microscopic imaging system, include above-mentioned adhesive structure. Also relates to an installation method of the fluorescence microscopic imaging system, which is applied to the fluorescence microscopic imaging system.
Background
Fluorescence microscopy imaging systems typically require the use of various types of optical and electro-optical components, such as industrial cameras, tube lenses, objective lenses, filters of various bandwidths, diaphragms of various sizes, lenses for various purposes, focus drives, LD light sources, LED light sources, and the like.
The alignment accuracy of the above optical and optoelectronic components is high, and the direction and position accuracy cannot be guaranteed by directly using mechanical parts to support and assemble the optical and optoelectronic components, and generally, an adjustment compensation device needs to be added in the system to adjust the relative positions of the optical and optoelectronic components, so as to guarantee the performance of the system. Although the compensation device can improve the relative position precision of various optical and photoelectric elements, the arrangement of the compensation device can increase the difficulty in the adjustment of the system, reduce the assembly efficiency of the system and is inconvenient for system maintenance; on the other hand, the system is difficult to reduce in size, and various restrictions are imposed on the observation target of the system, for example, a biochip, thereby affecting the observation operation.
Disclosure of Invention
The invention aims to provide a bonding structure which can realize the adhesive fixation of two parts and also can effectively ensure the relative position precision of the two parts. It is another object of the present invention to provide a fluorescence microscopy imaging system comprising the above bonding structure. It is still another object of the present invention to provide a method for installing a fluorescence microscopy imaging system, which is applied to the fluorescence microscopy imaging system.
In order to achieve the above object, the present invention provides a bonded structure comprising a first member and a second member for adhesive fixation; the adhesive surfaces of the first component and the second component comprise a plurality of tenon-and-mortise splicing surfaces which are mutually matched and nested; any pair of tenon-and-mortise splicing surfaces which are mutually glued and at least one pair of tenon-and-mortise splicing surfaces which are mutually glued are distributed oppositely and are not vertical.
Preferably, a normal of any one mortise and tenon joint surface is perpendicular to the joint direction of the first component and the second component.
Preferably, all the mortise and tenon splicing surfaces comprise a flat gluing surface and an inclined gluing surface; the flat gluing surface is parallel to the first reference direction or the second reference direction; the inclined gluing surface is not parallel to the first reference direction and the second reference direction; and any two of the first reference direction, the second reference direction and the splicing direction are mutually vertical.
Preferably, a normal of any one mortise-tenon joint surface is parallel to the joint direction of the first component and the second component.
Preferably, all the mortise and tenon joint surfaces are distributed in multiple layers at intervals along the joint direction, and the projections of all the mortise and tenon joint surfaces are overlapped and superposed in a reference plane; the reference plane is perpendicular to the splicing direction.
Preferably, the end of the first component and the end of the second component are provided with tenon heads and tenon eyes in the shape of zipper teeth.
The invention also provides a fluorescence microscopic imaging system which comprises the bonding structure.
The invention also provides an installation method of the fluorescence microscopic imaging system, which is applied to the fluorescence microscopic imaging system and comprises the following steps:
assembling all parts of the fluorescence microscopic imaging system in groups; the first packet includes a camera support; the second sub-group comprises a barrel mirror bracket and an illumination assembly; the third packet comprises a voice coil motor component;
and the camera support and the tube mirror support are fixed in an adhesive mode to form the bonding structure, and the lighting assembly and the voice coil motor assembly are fixed in an adhesive mode to form the bonding structure.
Preferably, the step of fixing the camera support and the telescope support by gluing specifically includes:
photosensitive glue with the linear shrinkage rate not more than 0.1% is arranged between any pair of mortise and tenon joint splicing surfaces on the outermost sides of the camera support and the barrel mirror support;
after the photosensitive adhesive layer is cured, setting the shearing strength between any pair of mortise and tenon joint surfaces at the inner sides of the camera support and the cylindrical mirror support to be not less than 10N/mm 2 The structural adhesive of (1).
Preferably, the step of assembling all the parts of the fluorescence microscopy imaging system in groups comprises:
the industrial cameras and the camera supports are summarized into a first group, and the tube mirrors, the tube mirror supports and the lighting assemblies are summarized into a second group;
positioning and mounting the industrial camera and the camera bracket, and positioning and mounting the tube lens, the tube lens bracket and the lighting assembly;
the step of adhesively fixing the camera mount and the tube lens mount includes:
the camera bracket and the barrel mirror bracket are fixed by gluing at angles distributed at intervals between the industrial camera and the barrel mirror; wherein, be equipped with the dust ring between the clearance of industry camera with the section of thick bamboo mirror.
Against the above background, the present invention provides a bonded structure including a first member and a second member for adhesive fixation; the adhesive surfaces of the first component and the second component comprise a plurality of tenon-and-mortise splicing surfaces which are mutually matched and nested; any pair of tenon-and-mortise splicing surfaces which are mutually glued and at least one pair of tenon-and-mortise splicing surfaces which are mutually glued are distributed oppositely and are not vertical.
The bonding structure realizes adhesive fixation by utilizing a plurality of pairs of mortise-tenon splicing surfaces formed by occlusion splicing of the first component and the second component. Because the tenon fourth of twelve earthly branches concatenation face between first part and the second part distributes in opposite directions and out of plumb, consequently, the shrink of a plurality of glue films between the tenon fourth of twelve earthly branches concatenation face of different pairs and the harmomegathus that the glue film received the temperature variation and produce in the product use can offset the compensation each other, and then effectively overcome first part and second part because of the position drift that the colloid harmomegathus caused.
Therefore, the bonding structure effectively improves the assembly precision of the first component and the second component in the assembly process, weakens the interference of temperature on the relative position relation of the first component and the second component in the use process, and ensures the long-term position assembly precision of the first component and the second component.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.
FIG. 1 is a schematic diagram of a partial structure of a fluorescence microscopy imaging system according to an embodiment of the present invention;
FIG. 2 is a schematic structural view of a barrel lens holder and a camera holder provided in the present invention during an assembling process;
FIG. 3 is a schematic view of a first bonding structure provided by the present invention in a first direction;
FIG. 4 is a schematic view of a first bonding structure provided by the present invention in a second direction;
FIG. 5 is a cross-sectional view taken along line A-A of FIG. 4;
FIG. 6 is a schematic structural view of a second bonding structure provided in the present invention;
FIG. 7 is a cross-sectional view of FIG. 6;
FIG. 8 is a schematic flow chart of a method for installing a fluorescence microscopy imaging system according to an embodiment of the present invention;
fig. 9 is a partial schematic flow chart of a method for installing a fluorescence microscopy imaging system according to an embodiment of the present invention.
The system comprises an industrial camera 1, a camera 2, a camera support 3, a dustproof ring 4, a cylindrical lens 5, a cylindrical lens support 6, an illumination assembly 7, an objective lens seat 8, an objective lens 9, a voice coil motor assembly, a multi-degree-of-freedom adjusting device connecting piece 10, a detection device connecting piece 11, a mortise and tenon joint surface 20, a flat adhesive surface 201 and an inclined adhesive surface 202.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In order that those skilled in the art will better understand the disclosure, the invention will be described in further detail with reference to the accompanying drawings and specific embodiments.
Referring to fig. 1 to 9, fig. 1 is a schematic partial structure diagram of a fluorescence microscopy imaging system according to an embodiment of the present invention; FIG. 2 is a schematic structural view of a barrel lens holder and a camera holder provided in the present invention during an assembling process; FIG. 3 is a schematic view of a first bonding structure provided by the present invention in a first direction; FIG. 4 is a schematic view of a first bonding structure provided by the present invention in a second direction; FIG. 5 is a cross-sectional view taken along line A-A of FIG. 4; FIG. 6 is a schematic structural view of a second bonding structure provided in the present invention; FIG. 7 is a cross-sectional view of FIG. 6; FIG. 8 is a schematic flow chart of a method for installing a fluorescence microscopy imaging system according to an embodiment of the present invention; fig. 9 is a partial schematic flow chart of a method for installing a fluorescence microscopy imaging system according to an embodiment of the present invention.
The invention provides a bonding structure, which comprises a first component and a second component for adhesive fixation. The end part of the first component is provided with a plurality of bulges which are distributed at intervals, and the plurality of bulges enable the end part of the first component to form an inserting port with a mortise and a tenon; similarly, the end of the second component is provided with a plurality of bulges distributed at intervals, and the plurality of bulges enable the end of the second component to form a plug-in port with a mortise and a tenon. In the bonding structure, the plug port of the first component and the plug port of the second component can be nested in a matched mode.
Because the first component and the second component are fixed in an adhesive manner, the splicing position of the first component and the second component comprises a plurality of pairs of surfaces which are mutually attached, and an adhesive is arranged between the attaching gaps of the partial surfaces, the part surfaces which are fixed in an adhesive manner in the plurality of pairs of surfaces which are mutually attached are defined by the tenon-and-mortise splicing surfaces 20. Therefore, the mortise and tenon joint faces 20 mentioned herein need to satisfy both the condition of the first member and the second member being attached to each other and the condition of the adhesive connection.
In the bonding structure, the adhesive surfaces of the first component and the second component comprise a plurality of tenon-and-mortise splicing surfaces 20 which are mutually inosculated, nested and attached in pairs. Any pair of tenon-and-mortise splicing surfaces 20 which are mutually glued and at least another pair of tenon-and-mortise splicing surfaces 20 which are mutually glued are distributed oppositely and not vertical.
For example, the end of the first component is provided with a first surface and a second surface, the end of the second component is provided with a third surface and a fourth surface, and the first surface, the second surface, the third surface and the fourth surface are attached to each other in pairs when the insertion port of the first component and the insertion port of the second component are inserted into each other. Wherein, first face and the laminating of third face are in pairs and adhesive fixation, and the laminating of second face and fourth face is in pairs and adhesive fixation. For the above structure, the first surface, the third surface and the adhesive layer therebetween can be regarded as a plane, the second surface, the fourth surface and the adhesive layer therebetween can be regarded as another plane, and the two planes are distributed in opposite directions, for example, may be parallel to each other or inclined, but are not perpendicular to each other. Therefore, the shrinkage of the glue layers positioned on different planes in the curing process and the expansion and shrinkage of the glue layers caused by temperature change in the using process of a product can be mutually offset and compensated, so that the position drift of the first component and the second component caused by the expansion and shrinkage of the glue body can be effectively overcome, the assembling precision of the first component and the second component in the assembling process is effectively improved, the interference of the temperature on the relative position relation of the first component and the second component in the using process is weakened, and the long-term position assembling precision of the first component and the second component is ensured.
Obviously, the more the number of pairs of mortise and tenon joint surfaces 20 between the first component and the second component is, the different angles of the mortise and tenon joint surfaces 20 in different pairs are different, and the more probable the plurality of mortise and tenon joint surfaces 20 are close to an ideal value in the offset compensation of the position drift caused by the expansion and contraction of the colloid, that is, the better the offset compensation effect is. Of course, as the number of the mortise and tenon joint surfaces 20 between the first component and the second component increases, the processing difficulty and the assembling difficulty of the first component and the second component also increase, and therefore, the number and the angle of the mortise and tenon joint surfaces 20 between the first component and the second component can be designed according to the actual needs of different products on the first component and the second component.
In summary, the bonding structure provided by the present invention realizes the adhesive fixation by using the mortise-tenon joint surface 20 formed by the engagement type interface between the first component and the second component. Because the plurality of pairs of mortise-tenon joint surfaces 20 between the first component and the second component are distributed in opposite directions and have different angles, the stress and the volume change quantity generated by the glue layers between the plurality of pairs of mortise-tenon joint surfaces 20 can be mutually offset and compensated, the fixed connection between the first component and the second component is met, the position drift of the first component and the second component is greatly weakened or even avoided, and the long-term position precision of a product with the first component and the second component is ensured.
The following describes the bonding structure provided by the present invention with reference to the accompanying drawings and embodiments.
The following describes the bonding structure provided by the present invention in detail by two types of examples.
In a first example, the normal of any one mortise and tenon joint surface 20 is perpendicular to the joint direction of the first component and the second component, and reference can be made to fig. 3 to 5. The aforementioned splicing direction refers to the horizontal direction in fig. 3 and 4 and the direction perpendicular to the inside and outside of the paper in fig. 5. Therefore, for any mortise and tenon joint surface 20, it is parallel to the joint direction of the first component and the second component, i.e. perpendicular to the paper surface of fig. 5.
Wherein, all the mortise and tenon joint surfaces 20 can comprise a flat gluing surface 201 and an inclined gluing surface 202; the flat adhesive surface 201 refers to a horizontal or vertical surface in the paper surface of fig. 5, and the oblique adhesive surface 202 refers to a surface inclined in the paper surface of fig. 5.
For convenience of description, an XZY axis coordinate system may be established in the horizontal direction shown in fig. 5, the vertical direction, and the in-and-out direction of the paper of fig. 5. The Z-axis corresponds to the in-out direction of the paper of fig. 5, also referred to as the splice direction of the first and second members in this example; the X-axis and the Y-axis correspond to the horizontal direction and the vertical direction of fig. 5, respectively, also referred to as a first reference direction and a second reference direction in this example. Obviously, any two of the splicing direction, the first reference direction and the second reference direction are perpendicular to each other. Accordingly, the flat adhesion surface 201 refers to a surface parallel to the first reference direction or the second reference direction, and the oblique adhesion surface 202 refers to a surface not parallel to both the first reference direction and the second reference direction.
Taking as an example that all the shaded structures in fig. 5 correspond to the first component and all the blank frame bodies correspond to the second component, the first component and the second component both include 8 mortise and tenon joint surfaces 20, and the 16 mortise and tenon joint surfaces 20 are attached in pairs to form 8 pairs of mutually adhesive mortise and tenon joint surfaces 20. In the 8 pairs of mutually sticky mortise and tenon joint faces 20, any pair of mortise and tenon joint faces 20 is perpendicular to the paper surface of the picture 5, wherein 2 pairs of mortise and tenon joint faces 20 are horizontally distributed and are parallel to each other, 2 pairs of mortise and tenon joint faces 20 are vertically distributed and are parallel to each other, and in addition, 4 pairs of mortise and tenon joint faces 20 are obliquely distributed and are parallel to each other.
The displacement variation quantity of the 8 pairs of mortise and tenon joint surfaces 20 between the first component and the second component, which is generated by the expansion and contraction of the glue layer, can be decomposed to an X axis and a Y axis. Because the glue layer between any pair of mortise and tenon joint surfaces 20 may expand and contract, and the expansion and contraction amounts are different, the stresses in different directions generated by the 8 pairs of mortise and tenon joint surfaces 20 can be mutually offset and compensated. Compared with the method for achieving adhesive fixation of the first component and the second component by utilizing a single adhesive layer in a single direction, the scheme provided by the application is beneficial to improving the assembling precision of the first component and the second component in the X-axis direction and the Y-axis direction and the relative position precision during use by offsetting and compensating the expansion and contraction change of all the adhesive layers.
In a second example, the normal of any one mortise and tenon joint plane 20 is parallel to the jointing direction of the first component and the second component, and reference can be made to fig. 6 and 7. The aforementioned splicing direction corresponds to the direction of the oblique line from the upper left corner to the lower right corner in fig. 6, and also corresponds to the vertical direction in fig. 7. Therefore, for any mortise-tenon joint face 20, it is distributed along the horizontal direction of fig. 7.
Similar to the first example, for all the mortise and tenon joint surfaces 20 in the second example, it is possible to compensate for the mutual offset of the stress and the expansion and contraction changes by the plurality of glue layers between all the mortise and tenon joint surfaces 20, and avoid the position drift of the first component and the second component in the vertical direction of fig. 7.
For the second example, further, all the mortise and tenon splicing faces 20 may be distributed at intervals in multiple layers along the splicing direction. As shown in fig. 7, the first component and the second component respectively form 10 tenon-and-mortise joint surfaces 20 at three glue applying points, and the 20 tenon-and-mortise joint surfaces 20 are attached in pairs to form 10 pairs of tenon-and-mortise joint surfaces 20.
Meanwhile, the projections of the 10 pairs of mortise and tenon joint surfaces 20 overlap and coincide on the horizontal plane of fig. 7. The horizontal plane of fig. 7 can be regarded as a reference plane of the second example, and the reference plane is perpendicular to the splicing direction between the first member and the second member. This arrangement is directed to the first and second components having a tendency to move away from and close to each other in the vertical direction of fig. 7, so that the projections of the 10 pairs of mortise and tenon joint surfaces 20 on the reference surface overlap each other on the premise that the 10 pairs of mortise and tenon joint surfaces 20 are fixed to each other by adhesion, thereby further reducing the risk of the first and second components breaking away from each other at the position where the adhesive is adhered.
The relative position arrangement of the multi-layer mortise-tenon joint surfaces 20 is equivalent to that safety redundancy is formed at the bonding position of the first component and the second component, so that risks which are difficult to control in the bonding process can be avoided, and adverse interference caused by factors such as glue amount, curing temperature and humidity, vacuoles formed during gluing and the like on the bonding effect can be avoided.
Of course, the above example only illustrates the case where the mortise and tenon joint surface 20 is provided 10 pairs between the first member and the second member. As for the number of the mortise and tenon joint surfaces 20 between the first component and the second component and other relative relations except the angle, the mortise and tenon joint surfaces can be designed specifically according to actual needs.
For example, in fig. 7, the end of the first member and the end of the second member are respectively provided with an element-shaped tenon portion and an element-shaped mortise portion, so that the end of the first member and the end of the second member are inserted and connected with each other.
On the basis of any one of the embodiments, the invention also provides a fluorescence microscopic imaging system. According to the number and the assembly relation of the parts of the fluorescence microscopic imaging system, a group of the bonding structures can be arranged in the fluorescence microscopic imaging system, and two groups or even multiple groups of bonding structures can be arranged in the fluorescence microscopic imaging system. As for the specific form of the bonding structure between different parts, the specific form can be set according to the assembly relationship, the motion characteristic and the assembly precision requirement of the specific parts.
Taking a fluorescence microscopic imaging system with a camera bracket 2, a tube mirror bracket 5, an illumination assembly 6 and a voice coil motor assembly 9 as an example, in the fluorescence microscopic imaging system, the end of the camera bracket 2 and the end of the tube mirror bracket 5 are mutually attached and fixed, and the end of the illumination assembly 6 and the end of the voice coil motor assembly 9 are mutually attached and fixed.
For the camera mount 2 and the tube lens mount 5, the camera mount 2 is usually connected with the industrial camera 1 in a positioning manner so as to be relatively fixed, the tube lens mount 5 is usually connected with the illumination assembly 6 and the tube lens 4 in a positioning manner so as to be relatively fixed, and the industrial camera 1 and the tube lens 4 have high requirements on the distance along the axial direction and the angle of the axial line, so that the requirements on the distance along the axial direction and the angle of the axial line of the camera and the tube lens mount 5 are high.
Therefore, when the above-mentioned bonding structure satisfies the joint fixation of the camera holder 2 and the barrel mirror holder 5, the visible camera holder 2 and the barrel mirror holder 5 are respectively a first component and a second component, and the normal of the mortise and tenon joint surface 20 between the first component and the second component is perpendicular to the joint direction of the first component and the second component. Thus, the adhesive layer between the camera holder 2 and the tube lens holder 5 hardly affects the details of the camera holder 2 and the tube lens holder 5 in the axial direction, and it is not necessary to consider the curing shrinkage in the axial direction and the expansion and shrinkage change due to the temperature change. As for the angles of the axes of the camera holder 2 and the tube mirror holder 5, in practice, measurement and examination are usually performed in the horizontal direction and the vertical direction as shown in fig. 5, so that compensation of stress and expansion and contraction changes can be realized by using the glue layer between the camera holder 2 and the tube mirror holder 5, in short, the stress and displacement of the glue layer with different angles are superimposed to approach an ideal value such as 0, and then the angles of the axes of the camera holder 2 and the tube mirror holder 5 approach the ideal value such as coincidence.
For the illumination assembly 6 and the voice coil motor assembly 9, the illumination assembly 6 is generally in positioning connection with the barrel mirror bracket 5 and the barrel mirror 4 to be relatively fixed, and the voice coil motor assembly 9 is generally in positioning connection with the objective lens holder 7 and the objective lens 8 to be relatively fixed. Therefore, when the illumination assembly 6 and the voice coil motor assembly 9 are assembled and connected, in addition to ensuring the relative angle of the axial direction of the cylindrical lens 4 and the objective lens 8, the adhesive strength and the vibration resistance and suppression effect of the connection part of the illumination assembly 6 and the voice coil motor assembly 9 when the objective lens 8 makes the linear reciprocating motion are also satisfied.
Therefore, when the above-mentioned adhesive structure satisfies the attachment fixation of the illumination assembly 6 and the voice coil motor assembly 9, the visual illumination assembly 6 and the voice coil motor assembly 9 are the first member and the second member, respectively. The normal line of the tenon-and-mortise splicing surface 20 between the first component and the second component is parallel to the splicing direction of the second component and the second component; all the mortise and tenon joint surfaces 20 are distributed in multiple layers at intervals along the joint direction machine of the first component and the second component, and the projections of all the mortise and tenon joint surfaces 20 are overlapped and superposed in the horizontal plane shown in fig. 7, namely the reference plane. Thus, the stress of the glue layer between the lighting module 6 and the voice coil motor module 9 and the volume change amount caused by expansion and contraction are mainly reflected in the splicing direction of the lighting module 6 and the voice coil motor module 9, the relative angle of the lighting module 6 and the voice coil motor module 9 in the axial direction is hardly influenced, the vibration energy can be better absorbed, the vibration conduction can be inhibited, and the anti-fatigue property is good. Of course, because the lighting assembly 6 and the voice coil motor assembly 9 are provided with the plurality of layers of mortise-tenon joint surfaces 20 and the glue layers thereof in the splicing direction, the stress generated by the glue layers and the volume change amount generated by expansion and contraction can be mutually offset and compensated, so that the position drift of the glue body to the bonded piece in the curing process and the using process can be effectively overcome.
In addition, because of the overlapping coincidence of all mortise and tenon joint surfaces 20 in the reference plane, when the voice coil motor assembly 9 does high-frequency linear reciprocating motion along the vertical direction of fig. 7, all the mortise and tenon joint surfaces 20 can be restricted and blocked, so that higher bonding strength is ensured, and the phenomenon that any pair of mortise and tenon joint surfaces 20 is separated is avoided.
The fluorescence microscopic imaging system is described above only by taking the camera holder 2 and the tube mirror holder 5, the illumination assembly 6 and the voice coil motor assembly 9 as an example. The bonding structure provided by the application can also be adaptively adjusted according to the structural shape and the functional requirements of a product with specific application.
Further, the present invention also provides a method for installing a fluorescence microscopy imaging system, which is applied to the fluorescence microscopy imaging system and comprises:
s1: all parts of the fluorescence microscopic imaging system are assembled in groups; the first group comprises a camera support 2; the second sub-group comprises the barrel mirror holder 5 and the illumination assembly 6; the third packet comprises a voice coil motor assembly 9;
s2: the camera mount 2 and the barrel mirror mount 5 are adhesively fixed to form a bonded structure, and the illumination assembly 6 and the voice coil motor assembly 9 are adhesively fixed to form a bonded structure.
The installation method of the fluorescence microscopic imaging system is characterized in that a plurality of parts are assembled after being classified and grouped, and the grouping is based on that individual parts with higher assembly requirements in all the parts are classified into different groups. For example, the fluorescent microscopic imaging system has a high requirement for assembling the camera holder 2 and the tube lens holder 5, and has a high requirement for assembling the illumination assembly 6 and the voice coil motor assembly 9, so that on the premise of considering the assembling relationship of all the parts, the four parts can be divided into three groups, and then the rest parts of the fluorescent microscopic imaging system can be installed to the three groups of parts according to the actual installation requirement, and finally, the camera holder 2 and the tube lens holder 5 in the three groups are adhered to each other, and the illumination assembly 6 and the voice coil motor assembly 9 in the three groups are adhered to each other in an adhesive connection manner.
Wherein the camera support 2 and the tube mirror support 5, the lighting assembly 6 and the voice coil motor assembly 9 are adhesively connected according to the above-mentioned adhesive structure. The specific operations can be referred to the above description, and are not repeated herein.
On the basis of the above embodiment, for the installation method of the fluorescence microscopy imaging system provided by the present invention, the step of adhesively fixing the camera support 2 and the tube lens support 5 may specifically include:
s21: a photosensitive adhesive layer with the linear shrinkage rate not more than 0.1 percent is arranged between any pair of tenon-and-mortise splicing surfaces 20 on the outermost sides of the camera support 2 and the barrel mirror support 5;
s22: after the photosensitive adhesive layer is cured, the shearing strength is set to be not less than 10N/mm between any one pair of mortise-tenon joint splicing surfaces 20 at the inner sides of the camera bracket 2 and the cylindrical mirror bracket 5 2 The structural adhesive layer.
The steps are that the camera support 2 and the tube mirror support 5 are connected in a positioning mode through the photosensitive adhesive layer with the small shrinkage rate, and the fixed connection strength between the camera support 2 and the tube mirror support 5 is strengthened through the structural adhesive layer with the large adhesive strength.
Because the shrinkage factor of photosensitive glue is generally lower than that of structural glue, and the solidification speed is fast, be favorable to realizing quick, accurate preliminary fixed connection at camera support 2 and a section of thick bamboo mirror support 5, avoided factors such as external vibration, noise to the influence of the relative position of industrial camera 1 and a section of thick bamboo mirror support 5 in follow-up in-process. Considering that the adhesive strength of the photosensitive adhesive used in the present invention hardly satisfies the requirement of the camera holder 2 and the barrel mirror holder 5 for the fixing connection strength, the above steps also utilize the shear strength of not less than 10N/mm 2 The structural adhesive further strengthens adhesive bonding to the positioned camera support 2 and the barrel mirror support 5, and ensures the fixed connection strength of the camera support 2 and the barrel mirror support 5.
Based on the structural configuration of current camera support 2 and a section of thick bamboo mirror support 5, preferentially adopt photosensitive glue to set up photosensitive glue layer in the outside of camera support 2 and the two junction of a section of thick bamboo mirror support 5 to carry out preliminary location connection to camera support 2 and a section of thick bamboo mirror support 5 better.
The above-mentioned concrete steps have synthesized the characteristic of different kinds of adhesive, have guaranteed the position precision of camera support 2 and tube lens support 5 as the bonded piece, have guaranteed the bonding strength of the aforesaid bonded piece again.
Referring to fig. 5, in fig. 5, all the mortise and tenon joint surfaces 20 of the camera support 2 and the barrel mirror support 5 are distributed along an L-shaped path, two pairs of mortise and tenon joint surfaces 20 on the outermost side in the mortise and tenon joint surfaces 20 respectively refer to a pair of mortise and tenon joint surfaces 20 on the upper right corner and a pair of mortise and tenon joint surfaces 20 on the lower left corner in fig. 5, and on the contrary, the mortise and tenon joint surfaces 20 on the inner side in the mortise and tenon joint surfaces 20 refer to 6 pairs of mortise and tenon joint surfaces 20 located in the middle in fig. 5. The photosensitive adhesive layer formed by curing the photosensitive adhesive is positioned between any pair of tenon-and-mortise splicing surfaces 20 on the outermost side in all the tenon-and-mortise splicing surfaces 20, and the structural adhesive layer formed by curing the structural adhesive is positioned between any pair of tenon-and-mortise splicing surfaces 20 on the inner side in all the tenon-and-mortise splicing surfaces 20.
The above steps can also be regarded as further limiting the adhesive construction between the camera holder 2 and the telescope holder 5. For example, for a bonding structure using the camera support 2 and the barrel mirror support 5 as the first component and the second component, a photosensitive adhesive layer formed by curing a photosensitive adhesive is disposed between any pair of tenon-and-mortise splicing surfaces 20 on the outermost side of all the tenon-and-mortise splicing surfaces 20, and a structural adhesive layer formed by curing a structural adhesive is disposed between any tenon-and-mortise splicing surface 20 on the inner side of all the tenon-and-mortise splicing surfaces 20. Similarly, this particular gluing step and the glued structure formed by this step can also be applied to the lighting assembly 6 and the voice coil motor assembly 9.
Further, the step of assembling all the components of the fluorescence microscopy imaging system in groups may include:
s11: the industrial camera 1 and the camera support 2 are grouped into a first group, and the barrel mirror 4, the barrel mirror support 5 and the lighting assembly 6 are grouped into a second group;
s12: positioning and mounting an industrial camera 1 and a camera bracket 2, and positioning and mounting a cylindrical mirror 4, a cylindrical mirror bracket 5 and an illumination assembly 6;
the step of adhesively fixing the camera holder 2 and the tube lens holder 5 includes:
the camera bracket 2 and the tube lens bracket 5 are fixed by gluing at angles distributed at intervals between the industrial camera 1 and the tube lens 4; wherein, a dustproof ring 3 is arranged between the gap between the industrial camera 1 and the tube lens 4.
In contrast to the previous embodiment, the second group in this example also includes the telescopic mirror 4 mounted to the telescopic mirror mount 5, and therefore, when the first and second groups are relatively fixed by the camera mount 2 and the telescopic mirror mount 5, there is a certain relative positional relationship between the telescopic mirror 4 and the industrial camera 1.
In consideration of the actual assembly and operation links of the fluorescence microscopic imaging system, the requirements on the relative direction and the position of the industrial camera 1 and the cylindrical mirror 4 are high, and the relative position of the industrial camera 1 and the cylindrical mirror 4 is often required to be continuously adjusted through subsequent assembly and adjustment operations, so that when the first group and the second group are relatively fixed, a certain gap is reserved between the industrial camera 1 and the cylindrical mirror 4, and the dustproof ring 3 is arranged at the gap, so that the subsequent assembly and adjustment operations of the industrial camera 1 and the cylindrical mirror 4 are ensured, and the phenomenon that dust enters the fluorescence microscopic imaging system to affect the imaging effect of the fluorescence microscopic imaging system can be avoided.
The installation method of the fluorescence microscopic imaging system reasonably distributes a plurality of optical and photoelectric elements of the fluorescence microscopic imaging system in a plurality of groups, then combines an external detection device and an external positioning tool to realize reasonable positioning and adhesive fixation of two adjacent groups, and then removes the external detection device and the external positioning tool after the adhesive fixation is finished. The external positioning tool may include a multi-degree-of-freedom adjustment device and a detection device, and as shown in fig. 2, only a multi-degree-of-freedom adjustment device connecting part 10 of the multi-degree-of-freedom adjustment device and a detection device connecting part 11 of the detection device are shown.
Because two adjacent groups are connected by adopting the bonding structure mentioned herein, the bonding strength can be ensured, and the position drift of the bonded piece caused by the shrinkage in the curing process of the colloid and the temperature change in the using process can be effectively overcome.
In conclusion, the fluorescent microscopic imaging system assembled by the installation method of the fluorescent microscopic imaging system has the characteristics of compact structure, small volume, convenience in integration, high assembly precision, good observation effect and the like. In addition, the fluorescence microscopic imaging system can effectively guarantee the long-term position accuracy of a plurality of optical and photoelectric elements, so the fluorescence microscopic imaging system also has the characteristic of no maintenance.
The fluorescence microscopic imaging system, the installation method and the bonding structure thereof provided by the invention are described in detail above. The principles and embodiments of the present invention are explained herein using specific examples, which are presented only to assist in understanding the method and its core concepts. It should be noted that, for those skilled in the art, without departing from the principle of the present invention, it is possible to make various improvements and modifications to the present invention, and those improvements and modifications also fall within the scope of the claims of the present invention.
Claims (10)
1. A bonded structure comprising a first member and a second member for adhesive attachment; the adhesive surfaces of the first component and the second component comprise a plurality of tenon-and-mortise splicing surfaces (20) which are mutually matched and nested; any pair of tenon-and-mortise splicing surfaces (20) which are mutually glued and at least another pair of tenon-and-mortise splicing surfaces (20) which are mutually glued are distributed in opposite directions and are not vertical.
2. A bonding structure according to claim 1, wherein the normal of any of said mortise and tenon joint faces (20) is perpendicular to the joint direction of said first member and said second member.
3. The bonding structure according to claim 2, wherein all of said mortise and tenon joint faces (20) comprise a flat gluing face (201) and an inclined gluing face (202); the flat gluing surface (201) is parallel to a first reference direction or a second reference direction; the oblique gluing surface (202) is not parallel to the first reference direction and the second reference direction; wherein any two of the first reference direction, the second reference direction and the splicing direction are perpendicular to each other.
4. A bonded structure according to claim 1, wherein the normal to any of said mortise and tenon joint faces (20) is parallel to the joining direction of said first and second members.
5. The bonding structure according to claim 4, wherein all the mortise and tenon splicing surfaces (20) are distributed at intervals in multiple layers along the splicing direction, and the projections of all the mortise and tenon splicing surfaces (20) are overlapped and superposed in a reference plane; the reference plane is perpendicular to the splicing direction.
6. The bonding structure according to claim 5, wherein each of the end portion of the first member and the end portion of the second member is provided with a tenon portion and a mortise portion in a shape of a fastener element.
7. A fluorescence microscopy imaging system comprising a bonded structure according to any of claims 1 to 6.
8. A method of installing a fluorescence microscopy imaging system for use in the fluorescence microscopy imaging system of claim 7, comprising:
assembling all parts of the fluorescence microscopic imaging system in groups; the first group comprises a camera support (2); the second sub-group comprises a cylindrical mirror bracket (5) and a lighting assembly (6); the third sub-group comprises a voice coil motor assembly (9);
the camera bracket (2) and the barrel mirror bracket (5) are adhesively fixed to form the adhesive structure, and the illumination assembly (6) and the voice coil motor assembly (9) are adhesively fixed to form the adhesive structure.
9. The method for mounting a fluorescence microscopy imaging system according to claim 8, wherein the step of adhesively fixing the camera holder (2) and the tube lens holder (5) comprises in particular:
photosensitive glue with the linear shrinkage rate not more than 0.1% is arranged between any pair of mortise and tenon joint splicing surfaces (20) on the outermost sides of the camera support (2) and the barrel mirror support (5);
after the photosensitive adhesive layer is cured, setting the shearing strength between any pair of mortise and tenon joint surfaces (20) at the inner sides of the camera bracket (2) and the cylindrical mirror bracket (5) to be not less than 10N/mm 2 The structural adhesive of (1).
10. The method of installing a fluorescence microscopy imaging system according to claim 8, wherein the step of assembling all the components of the fluorescence microscopy imaging system in groups comprises:
the industrial camera (1) and the camera bracket (2) are grouped into a first group, and the barrel mirror (4), the barrel mirror bracket (5) and the lighting assembly (6) are grouped into a second group;
positioning and mounting the industrial camera (1) and the camera bracket (2), and positioning and mounting the tube lens (4), the tube lens bracket (5) and the lighting assembly (6);
the step of adhesively fixing the camera holder (2) and the tube lens holder (5) comprises:
the camera bracket (2) and the tube lens bracket (5) are fixed by gluing at angles distributed at intervals on the industrial camera (1) and the tube lens (4); wherein a dustproof ring (3) is arranged between the industrial camera (1) and the barrel mirror (4).
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