CN114808851A - Method for improving erosion resistance of embankment foundation soil body through microbial reaction solidification - Google Patents
Method for improving erosion resistance of embankment foundation soil body through microbial reaction solidification Download PDFInfo
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- 239000002689 soil Substances 0.000 title claims abstract description 110
- 230000000813 microbial effect Effects 0.000 title claims abstract description 72
- 238000000034 method Methods 0.000 title claims abstract description 38
- 238000006243 chemical reaction Methods 0.000 title claims abstract description 23
- 230000003628 erosive effect Effects 0.000 title claims abstract description 19
- 238000007711 solidification Methods 0.000 title claims description 18
- 230000008023 solidification Effects 0.000 title claims description 18
- 239000007788 liquid Substances 0.000 claims abstract description 49
- 238000005507 spraying Methods 0.000 claims abstract description 48
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims abstract description 19
- 239000004202 carbamide Substances 0.000 claims abstract description 19
- 239000001963 growth medium Substances 0.000 claims abstract description 16
- 230000035699 permeability Effects 0.000 claims abstract description 11
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims abstract description 10
- 229910001628 calcium chloride Inorganic materials 0.000 claims abstract description 10
- 239000001110 calcium chloride Substances 0.000 claims abstract description 10
- 238000012258 culturing Methods 0.000 claims abstract description 8
- 238000007789 sealing Methods 0.000 claims abstract description 8
- 241000589517 Pseudomonas aeruginosa Species 0.000 claims abstract description 4
- 238000007605 air drying Methods 0.000 claims abstract description 4
- 230000004913 activation Effects 0.000 claims abstract description 3
- 239000000243 solution Substances 0.000 claims description 43
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 230000001580 bacterial effect Effects 0.000 claims description 9
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 claims description 6
- 230000009471 action Effects 0.000 claims description 6
- 229910001424 calcium ion Inorganic materials 0.000 claims description 6
- 239000002245 particle Substances 0.000 claims description 6
- 230000008859 change Effects 0.000 claims description 5
- 238000012423 maintenance Methods 0.000 claims description 5
- 238000011088 calibration curve Methods 0.000 claims description 4
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 claims description 3
- 239000001888 Peptone Substances 0.000 claims description 3
- 108010080698 Peptones Proteins 0.000 claims description 3
- 238000009825 accumulation Methods 0.000 claims description 3
- 235000015278 beef Nutrition 0.000 claims description 3
- 238000004364 calculation method Methods 0.000 claims description 3
- 238000005094 computer simulation Methods 0.000 claims description 3
- 239000012153 distilled water Substances 0.000 claims description 3
- 239000011259 mixed solution Substances 0.000 claims description 3
- 235000019319 peptone Nutrition 0.000 claims description 3
- 239000002904 solvent Substances 0.000 claims description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 claims description 2
- 239000002609 medium Substances 0.000 claims description 2
- 244000005700 microbiome Species 0.000 claims 5
- 239000000126 substance Substances 0.000 description 6
- 239000012466 permeate Substances 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 238000010297 mechanical methods and process Methods 0.000 description 2
- 230000003204 osmotic effect Effects 0.000 description 2
- 238000000053 physical method Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000000087 stabilizing effect Effects 0.000 description 2
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 1
- 235000008733 Citrus aurantifolia Nutrition 0.000 description 1
- 235000011941 Tilia x europaea Nutrition 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 235000019270 ammonium chloride Nutrition 0.000 description 1
- -1 ammonium ions Chemical class 0.000 description 1
- 239000004568 cement Substances 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
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- 230000008595 infiltration Effects 0.000 description 1
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Classifications
-
- E—FIXED CONSTRUCTIONS
- E02—HYDRAULIC ENGINEERING; FOUNDATIONS; SOIL SHIFTING
- E02B—HYDRAULIC ENGINEERING
- E02B3/00—Engineering works in connection with control or use of streams, rivers, coasts, or other marine sites; Sealings or joints for engineering works in general
- E02B3/16—Sealings or joints
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- E—FIXED CONSTRUCTIONS
- E02—HYDRAULIC ENGINEERING; FOUNDATIONS; SOIL SHIFTING
- E02D—FOUNDATIONS; EXCAVATIONS; EMBANKMENTS; UNDERGROUND OR UNDERWATER STRUCTURES
- E02D3/00—Improving or preserving soil or rock, e.g. preserving permafrost soil
- E02D3/12—Consolidating by placing solidifying or pore-filling substances in the soil
Abstract
The invention belongs to the technical field of improving the erosion resistance of an embankment foundation soil body, and discloses a method for improving the erosion resistance of the embankment foundation soil body by solidifying a microbial reaction, which comprises the following steps of S1: selecting a basic culture medium to culture the pseudomonas aeruginosa; s2: firstly, performing activation culture on the strains, then inoculating the strains into a conical flask filled with a basic culture medium, and finally sealing and culturing; s3: preparing a cementing liquid by using calcium chloride and urea; s4: detecting microbial activity, namely detecting the microbial activity by using a conductivity meter; s5: spraying microbial liquid on the permeable embankment foundation soil, and spraying different modes according to different embankment foundation soil permeability coefficients; s6: slowly spraying a cementing solution on the surface of the embankment foundation soil body after the microbial solution is sprayed, and keeping for 6-12 hours after spraying; s7: repeating the steps S5 and S6 for 3-5 times, standing and air-drying; the method solves the problems of narrow application range, high economic cost and large influence on the environment of the method for improving the mechanical property of the soil body in the prior art, and is used for improving the erosion resistance of the soil body of the embankment foundation.
Description
Technical Field
The invention relates to the technical field of improving the erosion resistance of an embankment foundation soil body, in particular to a method for improving the erosion resistance of the embankment foundation soil body by curing through a microbial reaction.
Background
The infiltration damage is the main cause of instability damage of dikes, earth and rockfill dams, foundation pits and the like, and is one of the most harmful and serious disasters in the world. Piping is the main form of osmotic damage, and embankment piping damage is the most harmful natural disaster in osmotic damage. China belongs to continental monsoon climate, most rivers have the problem of safe flood crossing in summer due to non-uniform precipitation all the year round, and a plurality of river segment dikes or earth and rocky dams face huge piping threats when flood peaks cross the river, so that disasters caused by the piping are more frequent. Because piping damage has great and extensive harmfulness, how to improve the anti-erosion capability of the soil body is a problem which needs to be solved urgently.
The traditional soil improvement method can be mainly divided into a mechanical method, a physical method and a chemical method. The mechanical method is mainly characterized in that soil is compacted through mechanical rolling, so that the soil forms a compact whole, the compression, the shearing and the water resistance of the soil are enhanced, but the vibration is large in the construction process, and the soil is not suitable for being used in areas close to buildings and narrow areas; the physical method mainly refers to treating the soil by physical means such as heating, cooling and the like so as to achieve the purpose of stabilizing the soil, but the method has high economic cost and large influence on the surrounding environment; the chemical method is mainly characterized in that substances such as cement, lime and the like are added into soil to treat the soil, and the soil is improved through a series of chemical reactions or physicochemical reactions between chemical substances and soil particles, and between the chemical substances and the soil particles, so that the aim of stabilizing the soil is fulfilled.
The microbial geotechnical engineering is a new branch of geotechnical engineering, mainly uses the chemical combination reaction of microbes to solve the problems in the geotechnical engineering, and meets the engineering requirements by a method for improving the mechanical property of soil. Compared with the traditional method, the microbial reaction solidification technology increases cohesive force among soil body particles, has the advantages of controllable reaction process, low economic cost, environmental friendliness and the like, has great application potential and application value in the field of improving the anti-erosion characteristic of the soil body, and provides a new path for embankment base seepage-proofing treatment, emergency rescue of an embankment dam, operation maintenance and the like.
Disclosure of Invention
The invention aims to provide a method for improving the erosion resistance of an embankment foundation soil body by utilizing microbial reaction solidification, so as to solve the problems of narrow application range, high economic cost and large influence on the environment of the method for improving the mechanical property of the soil body in the prior art.
In order to achieve the above purpose, the invention provides the following technical scheme:
a method for improving the erosion resistance of an embankment foundation soil body by utilizing microbial reaction solidification comprises the following steps:
s1: culturing microbial liquid, namely selecting a basic culture medium to culture pseudomonas aeruginosa;
s2: performing activated culture on a strain slant, inoculating the strain to a conical flask filled with a basic culture medium by using an inoculating loop, sealing the conical flask, and finally culturing the conical flask in a constant-temperature shaking incubator;
s3: preparing a cementing liquid by using calcium chloride and urea;
s4: detecting microbial activity, namely measuring the microbial activity by using a conductivity meter, firstly calibrating a curve, adding NH4Cl solutions with different concentrations into a urea solution, recording conductivity values (mS/cm) when the read number is stable, and drawing a relation curve of the change value of ammonium ions and the change value of the conductivity; measuring and calculating microbial activity, adding the urea solution with the same concentration into a culture bacterial solution, recording conductivity values at different moments, and calculating the microbial activity through the calibration curve;
s5: spraying microbial liquid on the permeable embankment foundation soil body, and spraying the microbial liquid on the surface of the embankment foundation soil body in different modes according to different embankment foundation soil body permeability coefficients;
s6: slowly spraying a cementing solution on the surface of the embankment foundation soil body after the microbial solution is sprayed, maintaining the soil structure, and keeping for 6-12 hours after spraying to ensure that the microbial solution is fully contacted with the cementing solution;
s7: and repeating the steps S5 and S6 for 3-5 times, and then standing and air-drying to finish the solidification of the embankment foundation soil body.
Further, in S1 and S2, the basic medium formula used is: 3g of beef extract, 5g of peptone, 15 NaC 15 g, 1L of distilled water and 7.2 of pH value of a basic culture medium.
Furthermore, in S2, the activation culture time is 2h, the volume of the selected conical flask is 250mL, the basic culture medium in the conical flask is 100mL, when the conical flask is sealed in a sealing manner, the conical flask is firstly sealed by six layers of gauze and then sealed by newspaper, and finally the conical flask is placed in a constant-temperature shaking incubator at the temperature of 30 ℃ at 180r/min for culture for 24 h.
Further, in S3, the molar concentration ratio of calcium chloride to urea in the prepared cementitious solution was 2: 3.
Further, in S5, for the soil with a large permeability coefficient, since the embankment base soil is loose, the microbial solution permeates quickly, and the cultured microbial solution is directly and slowly sprayed on the surface of the embankment base soil with a leakage problem, for the embankment base soil with a small permeability coefficient, since the embankment base soil is dense and the microbial solution permeates slowly, the surface of the embankment base soil is drilled in advance, and then the microbial solution is sprayed on the surface of the embankment base soil, so that the microbial solution permeates from the surface of the embankment base soil and the inside of the embankment base soil.
Further, in S5, the specific method for spraying the microbial liquid on the surface of the embankment foundation soil body is as follows: and slowly and uniformly spraying the microbial liquid on the surface of the embankment foundation soil body at the maintenance environment temperature of 25 ℃ to enable the microbial liquid to slowly infiltrate downwards, fully contacting the microbial liquid with soil particles, and keeping for 6-12 hours to ensure that the microbes fully infiltrate the embankment foundation soil body.
Further, in S6, the volume of the sprayed cementitious solution is 0.5-2 times of the solvent amount of the sprayed microbial liquid, and the calculation model of the sprayed amount is as follows:
W＝S×F×T
in the formula: w is total amount sprayed, L; s is the surface area sprayed, m 2 (ii) a F is the spraying intensity, L/min. m 2 (ii) a T is the duration of spraying, s;
wherein, spraying intensity F is decided by the nozzle diameter, the action area and the natural moisture content of the soil body that use, and the computational model is:
in the formula: eta is the efficiency coefficient of the nozzle, and is generally 1.0-1.3; s is the surface area sprayed, m 2 (ii) a Omega is the water content of the soil body,%; d is the nozzle diameter, mm.
Further, in S6, the method of spraying the cementing liquid is: firstly, uniformly spraying a bacterial liquid or a mixed solution of calcium chloride and urea on the surface of an embankment base by using an adjustable spraying device; in order to improve the utilization rate of calcium ions, adding excessive urea solution so as to provide excessive carbonate ions to fully react the calcium ions; the surface of the dike base is dry at the beginning, the water content of the surface soil body rises along with the accumulation of the spraying amount of the cementing liquid, and the spraying amount is gradually reduced; in order to reduce the loss of the solution and ensure the utilization rate, the spraying is carried out according to the principle of a small amount of times and successive degressive action.
The beneficial effects of this technical scheme are:
1. according to the invention, a cementing and curing layer is formed by utilizing a microbial reaction, rapid anti-seepage treatment is carried out on the embankment foundation soil body, and a nutrient solution is adopted for maintenance after a bacterial colony is formed, so that the bacterial liquid dosage is reduced;
2. after the microbial reaction is reinforced, the strength, the rigidity and the water stability of the soil body can be improved, and the good air permeability of the soil body is maintained;
3. is beneficial to the growth of plants on the dike foundation, supplies water for the growth of the plants by improving the water retention capacity of soil, improves the quality of the soil and promotes the growth of the plants;
4. the whole process is convenient to control, wide in application working condition range, low in economic cost, free of influence on surrounding ecological environment and easy to popularize and apply.
Drawings
FIG. 1 is a flow chart of a method for improving the erosion resistance of an embankment foundation soil body by utilizing microbial reaction solidification;
Detailed Description
The invention is described in further detail below with reference to the following figures and embodiments:
as shown in fig. 1, a method for improving the erosion resistance of an embankment foundation soil body by using microbial reaction solidification comprises the following steps:
s1: culturing microbial liquid, and selecting a basic culture medium to culture pseudomonas aeruginosa, wherein the basic culture medium comprises the following formula: 3g of beef extract, 5g of peptone, 15 NaC 15 g, 1L of distilled water and 7.2 of pH value of a basic culture medium;
s2: activating and culturing the strain for 2 hours, inoculating the strain into a conical flask filled with a basic culture medium by using an inoculating loop, sealing the conical flask by using six layers of gauze, sealing the conical flask by using newspaper, and culturing the conical flask in a constant-temperature oscillation culture box at the temperature of 30 ℃ and at 180r/min for 24 hours, wherein the formula of the basic culture medium is the same as that of the basic culture medium in S1;
s3: preparing a cementing liquid by using calcium chloride and urea, wherein the molar concentration ratio of the calcium chloride to the urea is 2: 3;
s4: detecting microbial activity by using a conductivity meter, calibrating curves, and measuring NH with different concentrations 4 Adding Cl solution into urea solution, recording conductivity value (mS/cm) when reading number is stable, and drawing ammonium ion change value and conductivity changeTransforming a relation curve of values; measuring and calculating microbial activity, adding the urea solution with the same concentration into a culture bacterial solution, recording conductivity values at different moments, and calculating the microbial activity through the calibration curve;
s5: the microbial liquid is sprayed on the permeable embankment foundation soil body, and different modes are selected according to different embankment foundation soil body permeability coefficients to spray the microbial liquid on the surface of the embankment foundation soil body, and the specific method comprises the following steps: slowly and uniformly spraying microbial liquid to the surface of the embankment foundation soil body at the maintenance environment temperature of 25 ℃, enabling the microbial liquid to slowly infiltrate downwards, fully contacting the bacterial liquid with soil particles, and keeping for 6-12 h, wherein for the soil body with a larger permeability coefficient, because the embankment foundation soil body is loose, the microbial liquid infiltrates more quickly, the cultured microbial liquid is directly and slowly sprayed to the surface of the embankment foundation soil body with leakage problem, for the embankment foundation soil body with a smaller permeability coefficient, because the embankment foundation soil body is compact, the microbial liquid infiltrates more slowly, holes are drilled on the surface of the embankment foundation soil body in advance, and then the microbial liquid is sprayed to the surface of the embankment foundation soil body, so that the microbial liquid infiltrates from the surface of the embankment foundation soil body and the interior of the embankment foundation soil body;
s6: slowly spraying a cementing solution on the soil surface of the embankment base after the microbial solution is sprayed, keeping the soil structure, keeping for 6-12 hours after spraying, and enabling the microbial solution to be fully contacted with the cementing solution, wherein the volume of the sprayed cementing solution is 0.5-2 times of the solvent amount of the sprayed microbial solution, and the calculation model of the spraying amount is as follows:
W＝S×F×T
in the formula: w is total amount sprayed, L; s is the surface area sprayed, m 2 (ii) a F is the spraying intensity, L/min. m 2 (ii) a T is the duration of spraying, s;
wherein, spraying intensity F is decided by the nozzle diameter, the action area and the natural moisture content of the soil body that use, and the computational model is:
in the formula: eta is the nozzle efficiency coefficient, and is generally 1.0 to1.3; s is the surface area sprayed, m 2 (ii) a Omega is the water content of the soil body,%; d is the diameter of the nozzle, mm;
the method for spraying the cementing liquid comprises the following steps: firstly, uniformly spraying a bacterial liquid or a mixed solution of calcium chloride and urea on the surface of an embankment base by using an adjustable spraying device; in order to improve the utilization rate of calcium ions, adding excessive urea solution so as to provide excessive carbonate ions to fully react the calcium ions; the surface of the dike base is dry at the beginning, the water content of the surface soil body rises along with the accumulation of the spraying amount of the cementing liquid, and the spraying amount is gradually reduced; in order to reduce the loss of the solution and ensure the utilization rate, the spraying is carried out according to the principle of a small amount of times and successive degressive action.
S7: and repeating the steps S5 and S6 for 3-5 times, and then standing and air-drying to finish the solidification of the embankment foundation soil body.
The above description is only an example of the present invention, and the common general knowledge of the technical solutions or characteristics known in the solutions is not described herein too much. It should be noted that, for those skilled in the art, without departing from the technical solution of the present invention, several variations and modifications can be made, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicability of the patent. The scope of the claims of the present application shall be determined by the contents of the claims, and the description of the embodiments and the like in the specification shall be used to explain the contents of the claims.
Claims (8)
1. A method for improving the erosion resistance of an embankment foundation soil body by utilizing microbial reaction solidification is characterized by comprising the following steps:
s1: culturing microbial liquid, namely selecting a basic culture medium to culture pseudomonas aeruginosa;
s2: performing activated culture on a strain slant, inoculating the strain to a conical flask filled with a basic culture medium by using an inoculating loop, sealing the conical flask, and finally culturing the conical flask in a constant-temperature shaking incubator;
s3: preparing a cementing liquid by using calcium chloride and urea;
s4: detecting microbial activity by using conductivity meterActivity, first calibration curve, different NH concentrations 4 Adding a Cl solution into a urea solution, recording a conductivity value (mS/cm) when the reading number is stable, and drawing a relation curve of an ammonium ion change value and a conductivity change value; measuring and calculating microbial activity, adding the urea solution with the same concentration into a culture bacterial solution, recording conductivity values at different moments, and calculating the microbial activity through the calibration curve;
s5: spraying microbial liquid on the permeable embankment foundation soil body, and spraying the microbial liquid on the surface of the embankment foundation soil body in different modes according to different embankment foundation soil body permeability coefficients;
s6: slowly spraying a cementing solution on the soil surface of the embankment base after the microbial solution is sprayed, maintaining the soil structure, and keeping for 6-12 hours after spraying to ensure that the microbial solution is fully contacted with the cementing solution;
s7: and repeating the steps S5 and S6 for 3-5 times, and then standing and air-drying to finish the solidification of the embankment foundation soil body.
2. The method for improving the erosion resistance of an embankment foundation soil body by utilizing the microbial reaction solidification according to claim 1, wherein the method comprises the following steps: in S1 and S2, the basic medium recipe used was: 3g of beef extract, 5g of peptone, 15 NaC 15 g, 1L of distilled water and 7.2 of pH value of a basic culture medium.
3. The method for improving the erosion resistance of an embankment foundation soil body by utilizing the microbial reaction solidification according to claim 1, wherein the method comprises the following steps: in S2, the activation culture time is 2h, the volume of the selected conical flask is 250mL, the basic culture medium in the conical flask is 100mL, when the conical flask is sealed in a sealing manner, six layers of gauze are firstly used for sealing, then the conical flask is sealed by newspaper, and finally the conical flask is placed in a constant-temperature shaking incubator at the temperature of 30 ℃ for culture for 24h at 180 r/min.
4. The method for improving the erosion resistance of an embankment foundation soil body by utilizing the microbial reaction solidification according to claim 1, wherein the method comprises the following steps: in S3, the molar concentration ratio of calcium chloride to urea in the prepared cementing liquid is 2: 3.
5. The method for improving the erosion resistance of an embankment foundation soil body by utilizing the microbial reaction solidification according to claim 1, wherein the method comprises the following steps: in S5, for the soil with a large permeability coefficient, the embankment foundation soil is loose, and the microorganism liquid infiltrates quickly, and the cultured microorganism liquid is directly and slowly sprayed on the surface of the embankment foundation soil with leakage problem, for the embankment foundation soil with a small permeability coefficient, the microorganism liquid infiltrates slowly because the embankment foundation soil is dense, and the hole is drilled on the surface of the embankment foundation soil in advance, and then the microorganism liquid is sprayed on the surface of the embankment foundation soil, so that the microorganism liquid infiltrates from the surface of the embankment foundation soil and the inside of the embankment foundation soil.
6. The method for improving the erosion resistance of an embankment foundation soil body by utilizing the microbial reaction solidification according to claim 5, wherein: in S5, the specific method of spraying the microbial liquid on the surface of the embankment foundation soil body is as follows: and slowly and uniformly spraying the microbial liquid on the surface of the embankment foundation soil body at the maintenance environment temperature of 25 ℃ to enable the microbial liquid to slowly infiltrate downwards, fully contacting the microbial liquid with soil particles, and keeping for 6-12 hours to ensure that the microbes fully infiltrate the embankment foundation soil body.
7. The method for improving the erosion resistance of an embankment foundation soil body by utilizing the microbial reaction solidification according to claim 1, wherein the method comprises the following steps: in S6, the volume of the sprayed cementing liquid is 0.5-2 times of the solvent amount of the sprayed microbial liquid, and the calculation model of the spraying amount is as follows:
W＝S×F×T
in the formula: w is total amount sprayed, L; s is the surface area sprayed, m 2 (ii) a F is the spraying intensity, L/min. m 2 (ii) a T is the duration of spraying, s;
wherein, spraying intensity F is decided by the nozzle diameter, the action area and the natural moisture content of the soil body that use, and the computational model is:
in the formula: eta is the efficiency coefficient of the nozzle, and is generally 1.0-1.3; s is the surface area sprayed, m 2 (ii) a Omega is the water content of the soil body,%; d is the nozzle diameter, mm.
8. The method for improving the erosion resistance of an embankment foundation soil body by utilizing the microbial reaction solidification according to claim 7, wherein: in S6, the method of spraying the cementing liquid is as follows: firstly, uniformly spraying a bacterial liquid or a mixed solution of calcium chloride and urea on the surface of an embankment base by using an adjustable spraying device; in order to improve the utilization rate of calcium ions, adding excessive urea solution so as to provide excessive carbonate ions to fully react the calcium ions; the surface of the dike base is dry at the beginning, the water content of the surface soil body rises along with the accumulation of the spraying amount of the cementing liquid, and the spraying amount is gradually reduced; in order to reduce the loss of the solution and ensure the utilization rate, the spraying is carried out according to the principle of a small amount of times and successive degressive action.
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| US20080245272A1 (en) * | 2004-12-20 | 2008-10-09 | Kucharski Edward S | Microbial Biocementation |
| CN109797734A (en) * | 2019-01-14 | 2019-05-24 | 南京大学（苏州）高新技术研究院 | A kind of prevention and control of soil erosion method based on microbial mineralization effect |
| CN111254946A (en) * | 2020-01-16 | 2020-06-09 | 西北农林科技大学 | Method for reinforcing and protecting loess slope surface based on microbial mineralization |
| CN111501733A (en) * | 2020-04-21 | 2020-08-07 | 南京大学 | Method for solidifying soil body by utilizing in-situ microorganisms cultured in excitation mode |
| CN113882407A (en) * | 2021-11-11 | 2022-01-04 | 南京大学 | Landslide control method based on microbial mineralization |
| CN114345925A (en) * | 2021-12-20 | 2022-04-15 | 合肥工业大学 | Method for repairing desertified land and solidifying surface soil heavy metal by microorganisms |
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