CN114796048B - Preparation and application of traditional Chinese medicine extract for preparing antioxidant - Google Patents

Preparation and application of traditional Chinese medicine extract for preparing antioxidant Download PDF

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CN114796048B
CN114796048B CN202210430082.8A CN202210430082A CN114796048B CN 114796048 B CN114796048 B CN 114796048B CN 202210430082 A CN202210430082 A CN 202210430082A CN 114796048 B CN114796048 B CN 114796048B
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extract
chinese medicine
preparing
traditional chinese
petroleum ether
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CN114796048A (en
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赵小超
廖承谱
李学坚
柴玲
商勋
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Suqian Hospital Of Traditional Chinese Medicine
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Abstract

The invention discloses a preparation method and application of a traditional Chinese medicine extract for preparing an antioxidant, wherein the preparation method comprises the following steps: 1. extracting; 2. and (5) extracting. The application of the traditional Chinese medicine extract which can be used for preparing the antioxidant and is prepared by the method is aimed at. The ghost lamp light extract is prepared by taking single dried whole plant of the tender spot as a raw material and carrying out the steps of reflux, extraction, concentration and the like. The extraction process is simple and the cost is low. The GC-MS and antioxidant activity experiment results show that 22 chemical components are identified from the ghost lamp extract, mainly comprising fatty acids, fatty alcohols and vitamin compounds, and the half-removal concentration IC50 of the fatty acids, fatty alcohols and vitamin compounds on DPPH free radicals and ABTS+ free radicals is 208.69 mug/mL and 146.00 mug/mL respectively.

Description

Preparation and application of traditional Chinese medicine extract for preparing antioxidant
Technical Field
The invention belongs to the technical field of natural antioxidants, and particularly relates to preparation and application of a traditional Chinese medicine extract for preparing an antioxidant.
Background
The antioxidant is food additive capable of preventing or delaying food oxidation, improving food stability and prolonging storage period, and in the field of medicine, the antioxidant is a substance capable of helping to capture and neutralize free radicals so as to remove damage of the free radicals to human bodies. The history of antioxidant use by humans is long and people have been recorded in China as early as the first of the principals on green tea (containing natural antioxidant tea polyphenols). Antioxidants are widely used in a variety of fields such as atherosclerosis, diabetes, kidney disease, and the like. At present, the antioxidant is mainly used for industrial production, food processing and storage, cosmetic daily product production and storage, prevention and treatment of various diseases and the like, is almost ubiquitous, and has wide application and huge use amount. Antioxidants are mainly classified into artificial synthetic antioxidants and natural antioxidants according to the production process. Along with the development of technology and the continuous improvement of the life quality of people, people pay more and more attention to health, and natural antioxidants are more and more favored. Compared with artificial antioxidants, the natural antioxidants have the advantages of strong antioxidant activity, natural health, high safety, wide source range and the like. Therefore, there is a greater need to develop natural antioxidants, which bring better and cheaper natural antioxidants to human health.
The podophyllum [ B. Tennellum (Hormem.) Fisch. Et Mey ] is also called as herba Euphorbiae Humifusae, herba Saussureae Involueratae, small Ma Erduo, which is the whole herb of Arnebiae plant tender spot, and has slightly bitter and astringent taste, mild nature, small toxicity, and lung meridian tropism, and has effects of relieving cough and stopping bleeding. Has long history of folk application and definite curative effect. During the visit and collection of data, a plurality of people's old traditional Chinese medicines are found to be applied in clinical treatment, the curative effect is remarkable, and some experienced old people can pick themselves frequently to be used as the need at intervals. The Chinese herbal medicine assembly, the Chinese big dictionary and the Chinese materia medica are all well documented: cough, stir-fry Jiao Zhi hematemesis). However, modern researches on ghost lighting have not been carried out at a later time, and are regrettably. Therefore, the invention has profound meaning and wide prospect, and is worthy of research.
In addition, the wheat field weed harm investigation is carried out in Yangzhou city of Jiangsu province and in a plurality of areas in Guangzhou province, and the results show that the wheat field weed harm investigation has ghost lights, is not harmful and has a certain medicinal value, but is regarded as weed harm farmland, and the medicinal value of the ghost lights is seriously overturned. The ghost lamp is used as a recorded medicinal material of folk common herb assembly and Chinese herbal, but falls into weed lines and rows nowadays, and has lost its use. If the research on ghost lights can be carried out as soon as possible, the mechanism is clear, the pesticide effect is determined, the value is realized, weeds can be changed into treasures, the agricultural load is reduced, the environmental pollution caused by excessive use of pesticides is reduced, the resources of a Chinese herbal medicine drug library can be expanded, scientific basis is provided for clinical medication, multiple purposes are realized, and the significance is profound. According to the above wheat field weed hazard investigation, the quantity of ghost lights can be judged to be huge, the ghost lights are quite difficult in the farmland impurity removal process, the excessive use of herbicide not only has great damage to the wheat field, but also has great increase to the environmental pollution, and the problem is very serious. The patent relates to the field of 'weeds' which can be solved, is beneficial to environmental protection, can also drive local economic development, and provides a great amount of cheaper Chinese medicinal materials with obvious effect for clinic.
Disclosure of Invention
The invention aims to provide a preparation method and application of a traditional Chinese medicine extract for preparing an antioxidant, which are mainly used for food processing and storage, cosmetic daily product production and storage, prevention and treatment of various diseases and the like.
A method for preparing a traditional Chinese medicine extract for preparing an antioxidant, comprising the following steps:
1. extracting: collecting dried whole herb of the ghost lamp, cutting into sections with length of 1-3cm, sieving with No. 1 sieve (10 mesh) to remove soil residue, adding 8-12 times of solvent per kg of the whole herb of the ghost lamp, reflux extracting at constant temperature for 2-4 times, each time for 1-2.5 hr to obtain solvent extractive solution, mixing extractive solutions, concentrating under reduced pressure to obtain concentrate;
2. extraction: adding distilled water with the weight of 6-10 times of the concentrate obtained in the step one for suspension, suspending by an ultrasonic oscillator to obtain suspension, adding petroleum ether with the volume of 1-3 times of the suspension for extraction for 2-4 times, combining petroleum ether extract liquid, and recovering solvent under reduced pressure to obtain petroleum ether extract, wherein the petroleum ether extract is the traditional Chinese medicine extract.
Preferably, the solvent in the first step is one of 95% ethanol, 75% ethanol and methanol.
Preferably, the traditional Chinese medicine extract comprises the following compounds in percentage by weight: 58.55% of fatty acid, 7.5% of fatty alcohol, 10.03% of fat-soluble vitamin, 8.8% of coumarin, 3% of lignans, 9.32% of esters and 2.8% of amino acid.
Preferably, the fatty acid comprises palmitic acid, eicosapentaenoic acid, docosahexaenoic acid, N-formyl-L-tyrosine, linolenic acid, trans-13-octadecenoic acid, octadecadienoic acid and alpha-linolenic acid.
Preferably, the fatty alcohols include phytol, isophytol, phytol, inositol and tetrahydrolinalool.
Preferably, the fat-soluble vitamin is tocopherol, the coumarin is 5, 7-dimethoxy coumarin, the lignans comprise phyllanthin, and the amino acid comprises 8-azaxanthine.
Preferably, the esters include dimethyl oxalate, isopropyl acetate, gamma-dodecalactone, butyl 2-methylbutyrate and hexanoic anhydride.
Preferably, in the first step, 10 liters of extraction solvent is added into each kilogram of the all-herbal medicine material for lighting, and the extraction is performed under constant temperature reflux for 3 times, and each reflux is performed for 2 hours.
Preferably, the constant temperature in the first step is 80 ℃.
Preferably, in the second step, 8 times of distilled water is added to the concentrate obtained in the first step, the suspension is obtained by suspending with an ultrasonic oscillator, and petroleum ether with the volume being 2 times of that of the suspension is added to extract for 3 times.
The application of the traditional Chinese medicine extract for preparing the antioxidant, which is prepared by the method, is used for preparing the antioxidant, and the antioxidant is used for food processing storage, cosmetic daily product production storage, prevention and treatment of various diseases and the like.
The invention has the beneficial effects that: the ghost lamp extract is prepared by taking single dried whole plant of the tender spot as a raw material (the plant is identified as the tender spot plant of lithospermaceae by a research Huang Yunfeng assistant in Guangxi Chinese medicine research in Cao Jixiang field in the Suzhou Kogyo Yuan region of Jiangsu province) and carrying out the steps of reflux, extraction, concentration and the like. The extraction process is simple and the cost is low. The GC-MS and antioxidant activity experiment results show that 22 chemical components are identified from the ghost lamp extract, mainly comprising fatty acids, fatty alcohols and vitamin compounds, and the half-removal concentration IC50 of the fatty acids, fatty alcohols and vitamin compounds on DPPH free radicals and ABTS+ free radicals is 208.69 mug/mL and 146.00 mug/mL respectively.
Drawings
Fig. 1 is a total ion flow chromatogram of the traditional Chinese medicine extract of the present invention.
FIG. 2 is a graph showing the DPPH radical scavenging ability of the petroleum ether moiety.
FIG. 3 is petroleum ether site vs. ABTS + Free radical scavenging ability is shown.
Detailed Description
Further advantages and effects of the present invention will become apparent to those skilled in the art from the disclosure of the present invention, which is described by the following specific examples.
Example 1
1. Extracting: collecting dried whole herb of Podophyllum album, cutting off, sieving with No. 1 sieve to remove soil residue, adding 10 times of 95% ethanol extraction solvent per kg of medicinal materials, reflux extracting at 80deg.C for 3 times each for 2 hr to obtain 95% ethanol solvent extract; mixing the extractive solutions, and concentrating under reduced pressure to obtain concentrate.
2. Extraction: adding distilled water with the weight 8 times of the concentrate to suspend, suspending by an ultrasonic oscillator to obtain suspension, adding petroleum ether with the volume 1.5 times of the suspension to extract for 3 times, combining petroleum ether extract liquid, and recovering solvent under reduced pressure to obtain petroleum ether extract (namely the product of the invention).
Example 2
1. Extracting: collecting dried whole herb of the ghost lamp, cutting off, removing soil residues by a No. 1 sieve with a length of about 1cm, adding 8 times of 75% ethanol extraction solvent per kilogram of medicinal materials, and reflux-extracting at constant temperature for 2 times for 1 hour each time to obtain 75% ethanol solvent extract; mixing the extractive solutions, and concentrating under reduced pressure to obtain concentrate.
2. Extraction: adding distilled water with the weight of 6 times of the concentrate to suspend, suspending by an ultrasonic oscillator to obtain suspension, adding petroleum ether with the volume of 1 time of the suspension to extract for 2 times, combining petroleum ether extract liquid, and recovering solvent under reduced pressure to obtain petroleum ether extract (namely the product of the invention).
Example 3
1. Extracting: collecting dried whole herb of the ghost lamp, cutting off, removing soil residues by a No. 1 sieve with a length of about 2cm, adding 9 times of 95% ethanol extraction solvent per kilogram of medicinal materials, and reflux-extracting at constant temperature for 3 times for 2 hours each time to obtain 95% ethanol solvent extract; mixing the extractive solutions, and concentrating under reduced pressure to obtain concentrate.
2. Extraction: adding distilled water with the weight being 7 times of that of the concentrate to suspend, suspending by an ultrasonic oscillator to obtain suspension, adding petroleum ether with the volume being 2 times of that of the suspension to extract for 3 times, combining petroleum ether extract liquid, and recovering solvent under reduced pressure to obtain petroleum ether extract (namely the product of the invention).
Example 4
1. Extracting: collecting dried whole herb of the ghost lamp, cutting off, removing soil residues by a No. 1 sieve, adding 12 times of methanol extraction solvent into each kilogram of medicinal materials, and reflux-extracting at constant temperature for 4 times for 2.5 hours each time to obtain methanol solvent extract; mixing the extractive solutions, and concentrating under reduced pressure to obtain concentrate.
2. Extraction: adding distilled water 10 times of the weight of the concentrate to suspend, suspending by an ultrasonic oscillator to obtain suspension, adding petroleum ether 3 times of the volume of the suspension to extract for 4 times, combining petroleum ether extract liquid, and recovering solvent under reduced pressure to obtain petroleum ether extract (namely the product of the invention).
Example 5
Component analysis detection conditions:
GC conditions: capillary chromatographic column [ model DB-WAX UI, specification 30M (length). Times.0.25 mm (inner diameter). Times.0.25 μm (film thickness) ] with polyethylene glycol (PEG-20M) as stationary phase; column temperature: taking 40 ℃ as an initial temperature, selecting a programmed temperature (3 ℃/min to 250 ℃) and setting for 30 min; column flow rate: setting the speed to be 1.0 mL/min; sample inlet temperature: 250. the temperature is lower than the temperature; column front pressure: 100 kPa; sample injection amount: 1.0. Mu.L; split ratio: 10:1; carrier gas: high purity helium.
MS conditions: ionization mode: EI; electron energy: 70 eV; transmission line temperature: 250. the temperature is lower than the temperature; ion source temperature: 230. the temperature is lower than the temperature; four-stage bar temperature: 150. the temperature is lower than the temperature; the mass range is as follows: 35-500; the relative content of each main component is calculated by a chromatographic peak area normalization method. Qualitative was retrieved using a NIST11 standard mass spectrometry library computer.
The relative percentages of the components are calculated by an area normalization method through NIST11 standard mass spectrum library computer retrieval and comparison, and the results are shown in Table 1. 22 main chemical components are identified by total separation, wherein the main chemical components with higher content are palmitic acid (26.19%), trans-13-octadecenoic acid (22.92%), tocopherol (10.03%), 5, 7-dimethoxy coumarin (8.80%), inositol (4.55%), and fatty acid, fatty alcohol, fat-soluble vitamin and coumarin compounds are mainly used.
TABLE 1 GC-MS analysis results Table of Petroleum ether portion
Sequence number Retention time Names of Compounds Molecular formula Relative content (%)
1 38.98 Oxalic acid dimethyl ester C4H6O4 1.47
2 41.27 Maleic anhydride C12H22O3 1.29
3 48.96 Isopropyl acetate C5H10O2 1.95
4 48.99 Gamma-dodecalactone C12H22O2 2.59
5 50.72 2-Methylbutanoic acid butyl ester C9H16O2 2.02
6 55.09 Phyllanthus urin C14H12O3 3.00
7 55.53 Phytol (phyllanthus urines) C20H40O 0.40
8 55.55 Isophytol C20H40O 0.74
9 55.55 Plant alcohols C20H40O 1.06
10 61.43 Palmitic acid C16H32O2 26.19
11 61.46 Eicosapentaenoic acid C20H30O2 0.88
12 61.46 Docosahexaenoic acid C22H32O2 1.37
13 62.65 N-formyl-L-tyrosine C10H11NO4 1.54
14 63.10 Inositol (inositol) C6H12O6 4.55
15 63.15 Linoleic acid C18H30O2 0.99
16 66.05 Tetrahydrolinalool C10H22O 0.75
17 66.60 Trans-13-octadecenoic acid C18H34O2 22.92
18 67.68 Octadecadienoic acid C18H32O2 0.63
19 69.12 Alpha-linolenic acid C18H30O2 4.03
20 69.52 5, 7-Dimethoxycoumarin C11H10O4 8.80
21 75.32 8-azaxanthines C4H3N5O2 2.80
22 90.60 Tocopherols C29H50O2 10.03
Example 6
Antioxidant Activity assay:
6.1 DPPH free radical scavenging capacity experimental method
Preparing a test solution: the petroleum ether concentrate 100 mg is weighed, the absolute ethyl alcohol is fixed to a 10mg volumetric flask, the initial concentration is 1000 mug/mL, and the absolute ethyl alcohol is used for diluting the test solution with the concentration of 1000, 500, 250, 125, 62.5, 31.25, 15.26, 7.63 and 3.81 mug/mL by the multiple ratio before use for testing.
Preparation of VC positive control drug solution: the method is the same as the preparation of the test solution.
Preparation of DPPH standard solution: the DPPH of 25.4 and mg is weighed, dissolved by absolute ethyl alcohol and fixed in a volumetric flask of 100 mL for standby.
The standard solution is diluted by 10 times when in use; the experiment is set with 9 gradients, 20 mu L of each concentration of sample solution is respectively taken and added into a 96-well plate, 180 mu L of DPPH solution is added into each well in parallel, 3 parallel concentrations are set, and a control group and a blank group are simultaneously set. Well mixing, and placing the 96-well plate in a light-proof environment for reaction for 30min. Measured at a wavelength of 515 nm.
Clearance = [1- (A) i -A j )/A 0 ]´100%
Wherein: a is that 0 Absorbance values determined after mixing with 180 μl DPPH and 20 μl sample solvent; a is that i Absorbance values measured after mixing 180 μl DPPH with 20 μl sample; a is that j The absorbance was measured after 180. Mu.L of the sample solvent and 20. Mu.L of the sample were mixed.
6.2 ABTS + Free radical scavenging capacity experimental method
ABTS + Preparation of radical stock solution: 3mL of 7 mmol.L −1 ABTS and 3mL 2.45 mmol.L −1 Mixing potassium persulfate solution, standing overnight at room temperature in the dark to form ABTS + Free radical stock solution.
ABTS + The absorbance of the free radical stock solution was adjusted to 0.700.+ -. 0.005 by adding 70% ethanol before use. 40. Mu.L of sample was added to each well of a 96-well plate, 9 concentrations were experimentally set, and 160. Mu.LABSS was added + The radical working solution was mixed with 200. Mu.L of 70% ethanol as a blank, 10. 10 s was allowed to stand for 6 minutes, and absorbance was measured at 734. 734 nm wavelength.
Clearance = [1- (A) i -A j )/A 0 ]´100%
Wherein: a is that 0 To add 160. Mu.L of ABTS + And 40. Mu.L of sample solvent, and the absorbance value measured after mixing uniformly; a is that i 160. Mu.L ABTS + And an absorbance value measured after mixing the 40. Mu.L of the sample; a is that j The absorbance was measured after mixing 160. Mu.L of the sample solvent with 50. Mu.L of the sample.
Detecting by GC-MS to obtain total ion flow chromatogram as shown in figure 1.
The antioxidant activity results are shown in FIG. 2 and FIG. 3, and the petroleum ether extract has antioxidant activity on DPPH and ABTS + The free radical has stronger scavenging capability, and the IC50 is 208.69 mug/mL and 146.00 mug/mL respectively.
The product of the invention is used for preparing the antioxidant, is an antioxidant active part extract of the ghost lamp of the traditional Chinese medicine, and provides a raw material substance for the development of natural antioxidants, which is a technical problem which is mainly solved. The product of the invention has strong antioxidant activity and strong scavenging ability to DPPH and ABTS+free radical, and is an important component of natural antioxidants which are widely researched by domestic and foreign application. The product of the invention is a natural active extract of traditional Chinese medicine, has strong oxidation resistance and activity, small side effect and huge secondary development potential.
The above examples merely illustrate specific embodiments of the disclosure, but the embodiments of the disclosure are not limited by the foregoing. Any changes, modifications, substitutions, combinations, and simplifications that may be made without materially departing from the spirit and principles of the inventive concepts of the present disclosure are intended to be equivalent substitutes and are intended to be included within the scope of protection as defined by the claims.

Claims (5)

1. A preparation method of a traditional Chinese medicine extract for preparing an antioxidant is characterized by comprising the following steps: the method comprises the following steps:
1. extracting: collecting dried whole herb of the ghost lamp, cutting the segments to 1-3cm long, removing soil residues by a No. 1 sieve, adding 8-12 times of solvent into each kilogram of the whole herb of the ghost lamp, extracting at constant temperature for 2-4 times, each time for 1-2.5 hours to obtain solvent extract, combining the extract, and concentrating under reduced pressure to obtain a concentrate, wherein the solvent is one of 95% ethanol, 75% ethanol and methanol;
2. extraction: adding distilled water with the weight of 6-10 times of the concentrate obtained in the step one for suspension, suspending by an ultrasonic oscillator to obtain suspension, adding petroleum ether with the volume of 1-3 times of the suspension for extraction for 2-4 times, combining petroleum ether extract liquid, and recovering solvent under reduced pressure to obtain petroleum ether extract, wherein the petroleum ether extract is the traditional Chinese medicine extract.
2. The method for preparing a traditional Chinese medicine extract for preparing an antioxidant according to claim 1, wherein: in the first step, 10 liters of extraction solvent is added into each kilogram of all herbal medicine material for lighting, reflux extraction is carried out for 3 times at constant temperature, each reflux time is 2 hours, and the constant temperature is 80 ℃.
3. The method for preparing a traditional Chinese medicine extract for preparing an antioxidant according to claim 1, wherein: and in the second step, adding distilled water 8 times of the weight of the concentrate obtained in the first step for suspension, suspending by an ultrasonic oscillator to obtain suspension, and adding petroleum ether 2 times of the volume of the suspension for extraction for 3 times.
4. The herbal extract prepared by the method of claim 1.
5. The use of the extract of chinese medicine as defined in claim 1 for preparing antioxidants.
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Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20020010999A (en) * 2000-07-31 2002-02-07 김석남 The Extracts of Rosa davurica Pall Containing High Antioxidative Activity and Preparation Process of Flavan 3-ol Derivatives Using Their Extracts
CN102000132A (en) * 2010-11-22 2011-04-06 山东大学威海分校 Preparation method of traditional Chinese medicine preparation and antioxidation application thereof
CN102000133A (en) * 2010-11-22 2011-04-06 山东大学威海分校 Preparation method of traditional Chinese medicine anti-oxidation preparation
CN103417585A (en) * 2013-08-19 2013-12-04 重庆工商大学 Method for preparing antioxidants from cycas revoluta thunb leaves
CN103463163A (en) * 2013-09-23 2013-12-25 王琪 Application of Italy anchusa active part in preparation of antioxidant drugs, cosmetics and health care products
CN106177662A (en) * 2016-08-16 2016-12-07 余昌仕 A kind of treat prominent Chinese medicine composition of hyperosteogeny, intercalated disc and preparation method thereof
CN110420276A (en) * 2019-09-13 2019-11-08 广西中医药大学 Liposoluble constituent and its application as antioxidant in strong medicine field green onion

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20020010999A (en) * 2000-07-31 2002-02-07 김석남 The Extracts of Rosa davurica Pall Containing High Antioxidative Activity and Preparation Process of Flavan 3-ol Derivatives Using Their Extracts
CN102000132A (en) * 2010-11-22 2011-04-06 山东大学威海分校 Preparation method of traditional Chinese medicine preparation and antioxidation application thereof
CN102000133A (en) * 2010-11-22 2011-04-06 山东大学威海分校 Preparation method of traditional Chinese medicine anti-oxidation preparation
CN103417585A (en) * 2013-08-19 2013-12-04 重庆工商大学 Method for preparing antioxidants from cycas revoluta thunb leaves
CN103463163A (en) * 2013-09-23 2013-12-25 王琪 Application of Italy anchusa active part in preparation of antioxidant drugs, cosmetics and health care products
CN106177662A (en) * 2016-08-16 2016-12-07 余昌仕 A kind of treat prominent Chinese medicine composition of hyperosteogeny, intercalated disc and preparation method thereof
CN110420276A (en) * 2019-09-13 2019-11-08 广西中医药大学 Liposoluble constituent and its application as antioxidant in strong medicine field green onion

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