CN114794311A - Preparation method of sheep small intestine mucous membrane protein powder - Google Patents
Preparation method of sheep small intestine mucous membrane protein powder Download PDFInfo
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- CN114794311A CN114794311A CN202210139043.2A CN202210139043A CN114794311A CN 114794311 A CN114794311 A CN 114794311A CN 202210139043 A CN202210139043 A CN 202210139043A CN 114794311 A CN114794311 A CN 114794311A
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- enzymolysis
- intestinal mucosa
- protein powder
- small intestine
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- 239000000843 powder Substances 0.000 title claims abstract description 34
- 241001494479 Pecora Species 0.000 title claims abstract description 29
- 210000000813 small intestine Anatomy 0.000 title claims abstract description 28
- 108010052285 Membrane Proteins Proteins 0.000 title claims abstract description 24
- 102000018697 Membrane Proteins Human genes 0.000 title claims abstract description 23
- 238000002360 preparation method Methods 0.000 title claims abstract description 18
- 210000004400 mucous membrane Anatomy 0.000 title claims abstract description 16
- 230000000968 intestinal effect Effects 0.000 claims abstract description 17
- 238000001694 spray drying Methods 0.000 claims abstract description 13
- 102000004190 Enzymes Human genes 0.000 claims abstract description 12
- 108090000790 Enzymes Proteins 0.000 claims abstract description 12
- 238000001728 nano-filtration Methods 0.000 claims abstract description 11
- 230000009849 deactivation Effects 0.000 claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 238000001914 filtration Methods 0.000 claims abstract description 4
- 238000000034 method Methods 0.000 claims description 36
- 210000004347 intestinal mucosa Anatomy 0.000 claims description 34
- 102000004169 proteins and genes Human genes 0.000 claims description 19
- 108090000623 proteins and genes Proteins 0.000 claims description 19
- 239000000706 filtrate Substances 0.000 claims description 18
- 238000003756 stirring Methods 0.000 claims description 18
- 239000000243 solution Substances 0.000 claims description 15
- 108091005804 Peptidases Proteins 0.000 claims description 10
- 238000004140 cleaning Methods 0.000 claims description 9
- 239000004365 Protease Substances 0.000 claims description 7
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 7
- 235000019419 proteases Nutrition 0.000 claims description 7
- 108091005658 Basic proteases Proteins 0.000 claims description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 6
- 238000011033 desalting Methods 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 6
- 229920000669 heparin Polymers 0.000 claims description 5
- ZFGMDIBRIDKWMY-PASTXAENSA-N heparin Chemical compound CC(O)=N[C@@H]1[C@@H](O)[C@H](O)[C@@H](COS(O)(=O)=O)O[C@@H]1O[C@@H]1[C@@H](C(O)=O)O[C@@H](O[C@H]2[C@@H]([C@@H](OS(O)(=O)=O)[C@@H](O[C@@H]3[C@@H](OC(O)[C@H](OS(O)(=O)=O)[C@H]3O)C(O)=O)O[C@@H]2O)CS(O)(=O)=O)[C@H](O)[C@H]1O ZFGMDIBRIDKWMY-PASTXAENSA-N 0.000 claims description 5
- 229960001008 heparin sodium Drugs 0.000 claims description 5
- 238000000605 extraction Methods 0.000 claims description 4
- 102000035195 Peptidases Human genes 0.000 claims description 3
- 239000007864 aqueous solution Substances 0.000 claims description 3
- 238000011049 filling Methods 0.000 claims description 3
- 235000019833 protease Nutrition 0.000 claims description 3
- 150000003839 salts Chemical class 0.000 claims description 3
- 239000011780 sodium chloride Substances 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims 2
- 235000019621 digestibility Nutrition 0.000 claims 1
- 210000000936 intestine Anatomy 0.000 claims 1
- 239000000047 product Substances 0.000 abstract description 10
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 239000012528 membrane Substances 0.000 abstract description 2
- 108090000765 processed proteins & peptides Proteins 0.000 abstract description 2
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 abstract 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 abstract 2
- 241000282887 Suidae Species 0.000 abstract 2
- 210000001691 amnion Anatomy 0.000 abstract 2
- 229960000583 acetic acid Drugs 0.000 abstract 1
- 230000033228 biological regulation Effects 0.000 abstract 1
- 239000006227 byproduct Substances 0.000 abstract 1
- 230000007613 environmental effect Effects 0.000 abstract 1
- 239000012362 glacial acetic acid Substances 0.000 abstract 1
- 239000004519 grease Substances 0.000 abstract 1
- 239000000413 hydrolysate Substances 0.000 abstract 1
- 239000012535 impurity Substances 0.000 abstract 1
- 238000004321 preservation Methods 0.000 abstract 1
- 230000029219 regulation of pH Effects 0.000 abstract 1
- 241001465754 Metazoa Species 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 150000001413 amino acids Chemical group 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 235000019750 Crude protein Nutrition 0.000 description 2
- 241001673669 Porcine circovirus 2 Species 0.000 description 2
- 241001135549 Porcine epidemic diarrhea virus Species 0.000 description 2
- 241001135989 Porcine reproductive and respiratory syndrome virus Species 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 235000019784 crude fat Nutrition 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 210000004379 membrane Anatomy 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Birds (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a preparation method of sheep small intestine mucous membrane protein powder, which comprises the following steps: fresh amniotic membrane raw residues → water regulation of pH (glacial acetic acid and 400g/L sodium hydroxide) → enzyme addition → temperature regulation enzymolysis → enzyme deactivation (keeping at 90 ℃ for 20min) → gauze filtration (discarding filter residues) → enzyme hydrolysate → nanofiltration (100Da nanofiltration membrane) → reduced pressure concentration → spray drying → enzymolysis of finished products of the amniotic membrane proteins. The preparation method can efficiently utilize the sheep small intestine by-products in slaughterhouses to prepare the sheep small intestine mucous membrane into the intestinal membrane protein product, and the intestinal membrane protein product prepared from the sheep small intestine mucous membrane is used for feeding pigs, so that the potential safety hazard of homologous organisms is greatly reduced, the intestinal membrane protein product is free of impurities, the grease is removed, the preservation time is long, the small peptide content is high, the original odor is greatly eliminated, the sense of the product and the food calling performance of the pigs are improved, the production cost is low, and the preparation method is beneficial to environmental protection.
Description
Technical Field
The invention relates to the field of production of intestinal membrane protein powder, and particularly relates to a preparation method of sheep small intestine mucous membrane protein powder.
Background
The shortage of protein feed resources, especially high-quality protein feed resources, is a bottleneck which hinders the development of high-grade feed products in China, so that the development and production of high-quality protein feed raw materials have great practical significance. The mucous membrane of the small intestine of sheep is the leftover of the small intestine of sheep after extracting the heparin sodium, the protein content in dry matter exceeds 50 percent, the composition of amino acid is balanced, and the mucous membrane is an animal-derived protein raw material with higher nutritional potential, but due to structural reasons, animals cannot directly digest and absorb the mucous membrane, and the utilization rate is limited. The invention adopts the protease hydrolysis technology and the nano-scale membrane separation technology to solve the problems of poor product quality and high salt content, the product quality reaches the standard of imported like products, and the production cost is greatly lower than that of the imported like products.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide the preparation method of the sheep small intestine mucous membrane protein powder, which has the advantages of reasonable steps and convenient operation, and the prepared intestinal membrane protein powder has good quality and low salt content.
In order to achieve the purpose, the invention is realized by the following technical scheme:
(1) pretreatment of intestinal mucosa, namely collecting the intestinal mucosa by using fresh intestinal residues after heparin sodium extraction, adding a cleaning solution into a container to clean the intestinal mucosa, and filtering to obtain a clean intestinal mucosa after cleaning;
(2) performing enzymolysis, namely filling clean intestinal mucosa into an enzymolysis container, adding water into the enzymolysis container, then fully stirring by using a stirring device, adding protease into the enzymolysis container for enzymolysis, and performing enzyme deactivation after the enzymolysis is finished to obtain an enzymolysis solution;
(3) desalting, namely introducing the filtrate into a nanofiltration system for desalting to obtain desalted filtrate;
(4) concentrating, namely introducing the desalted filtrate into a double-effect reduced pressure concentrator for concentrating to obtain a concentrated solution;
(5) spray drying, namely adding terminal peptidase into the concentrated solution, and then carrying out spray drying to obtain the enzymolysis intestinal membrane protein powder.
Further, the cleaning solution in step 1 of the method is an aqueous solution containing 2.5% NaCl.
Further, in step 2 of the method, the mass ratio of the added substances to the intestinal mucosa in the enzymolysis container is 22-25: 100.
further, the temperature when the stirring device is used for fully stirring after water is added in the step 2 of the method is 40-50 ℃, the rotating speed during stirring is 300-600r/min, and the stirring time is 15-30 min.
Further, the protease added in step 2 of the method is alkaline protease (5000IU/g), and the mass ratio of the alkaline protease (5000IU/g) to the intestinal mucosa is 0.15: 100.
Further, in the method, in the step 2, the pH value is adjusted to 10 during enzymolysis, the temperature is adjusted to 52 ℃ during enzymolysis, and the temperature is kept for 4 hours at the temperature of 52 ℃.
Further, the enzyme deactivation in step 2 of the method is a high-temperature enzyme deactivation method.
Further, in the step 4 of the method, the filtrate is introduced into a nanofiltration system, and the nanofiltration operation conditions are that the operation pressure is 6bar, the feed liquid temperature is 55 ℃, and the pH of the feed liquid is 6.
Further, the negative pressure of the spray drying in the step 6 of the method is 0.25-0.40MPa, the inlet air temperature of the spray drying is 240-.
The intestinal membrane protein powder prepared by the method can effectively improve the biological safety and cannot cause secondary pollution. The prepared intestinal membrane protein powder can be completely dissolved in water.
The content of main nutritional indexes in the intestinal residue is shown in table 1.
TABLE 1 content of major nutritional indicators in intestinal dregs (on a dry matter basis)
| Composition (I) | Moisture content | Crude protein | Small peptides | Water-soluble protein |
| Content/% | 73.900 | 54.500 | 2.096 | 7.917 |
The hygiene indexes of the prepared sheep small intestine mucous membrane protein powder are shown in table 2.
TABLE 2 index of hygiene of protein powder of intestinal mucosa of sheep small intestine
| Total number of colonies | Mould fungus | Escherichia coli | Salmonella |
| ≦3000 | Not detected out | Not detected out | Not detected out |
100g of the prepared sheep small intestine mucous membrane protein powder is taken, and the detection result of the hydrolyzed amino acid is shown in table 3.
TABLE 3 detection results of hydrolyzed amino acids in intestinal mucosa protein powder of sheep small intestine
The analysis results of the main components of the prepared sheep small intestine mucous membrane protein powder are shown in table 4.
TABLE 4 analysis results of major components of sheep small intestine intestinal mucosa protein powder
| Crude protein | Crude fat | Moisture content | Coarse ash content | Lysine | Methionine |
| ≧70％ | ≦3％ | ≦8％ | ≦8％ | ≧3.85％ | ≧1.05％ |
And the obtained sheep small intestine mucous membrane protein powder is subjected to PRRSV, PCV2 and PEDV detection, and the detection result shows that the protein powder does not contain PRRSV, PCV2 and PEDV.
The sheep small intestine mucous membrane protein powder produced by the method has high protein solubility, is beneficial to digestion and absorption of animals, and is a high-quality animal feed, such as pig feed and the like. It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, it is intended that the present invention cover the modifications and variations of this invention provided they come within the scope of the appended claims and their equivalents.
Claims (10)
1. A preparation method of sheep small intestine mucous membrane protein powder is characterized by comprising the following steps: the method comprises the following steps:
(1) pretreatment of intestinal mucosa, namely collecting the intestinal mucosa by using fresh intestinal residues after heparin sodium extraction, adding a cleaning solution into a container to clean the intestinal mucosa, and filtering to obtain a clean intestinal mucosa after cleaning;
(2) performing enzymolysis, namely filling clean intestinal mucosa into an enzymolysis container, adding water into the enzymolysis container, then fully stirring by using a stirring device, adding protease into the enzymolysis container for enzymolysis, and performing enzyme deactivation after the enzymolysis is finished to obtain an enzymolysis solution;
(3) desalting, namely introducing the filtrate into a nanofiltration system for desalting to obtain desalted filtrate;
(4) concentrating, namely introducing the desalted filtrate into a double-effect reduced pressure concentrator for concentrating to obtain a concentrated solution;
(5) spray drying, namely adding terminal peptidase into the concentrated solution, and then carrying out spray drying to obtain the enzymolysis intestinal membrane protein powder.
2. The preparation method of the sheep small intestine intestinal mucosa protein powder according to claim 1, which is characterized in that: the cleaning solution in step 1 of the method is an aqueous solution containing 2.5% NaCl.
3. The preparation method of the sheep small intestine intestinal mucosa protein powder according to claim 1, which is characterized in that: in step 2 of the method, the mass ratio of the water added into the enzymolysis container to the intestinal mucosa is 22-25: 100.
4. the preparation method of the sheep small intestine intestinal mucosa protein powder according to claim 1, which is characterized in that: in the step 2 of the method, after water is added, the stirring device is used for fully stirring at the temperature of 40-50 ℃, the rotating speed of stirring is 300-600r/min, and the stirring time is 20-40 min.
5. The preparation method of the sheep small intestine intestinal mucosa protein powder according to claim 1, which is characterized by comprising the following steps: the protease added in step 2 of the method is alkaline protease (5000IU/g), and the mass ratio of the alkaline protease (5000IU/g) to the intestinal mucosa is 0.15: 100.
6. The preparation method of the sheep small intestine intestinal mucosa protein powder according to claim 1, which is characterized in that: in the step 2 of the method, the pH value is adjusted to 10 during enzymolysis, the temperature is adjusted to 52 ℃ during enzymolysis, and the temperature is kept for 4 hours at 52 ℃.
7. The preparation method of the sheep small intestine intestinal mucosa protein powder according to claim 1, which is characterized in that: the enzyme deactivation in step 2 of the method adopts a high-temperature enzyme deactivation method.
8. The preparation method of the sheep small intestine intestinal mucosa protein powder according to claim 1, which is characterized in that: in the step 3 of the method, the filtrate is introduced into a nanofiltration system, and the nanofiltration operation condition is that the operation pressure is 6bar, the feed liquid temperature is 55 ℃, and the pH of the feed liquid is 6.
9. The preparation method of the sheep small intestine intestinal mucosa protein powder according to claim 1, which is characterized in that: in the step 4 of the method, the desalted filtrate is introduced into a double-effect vacuum concentrator, the operation negative pressure is 0.4-0.5MPa, the temperature of the filtrate is heated to 75 ℃, and the filtrate is concentrated under the condition until the solid content is 45-48 wt%.
10. The preparation method of the sheep small intestine intestinal mucosa protein powder according to claim 1, which is characterized in that: in the step 5 of the method, the negative pressure of spray drying is 0.25-0.40MPa, the air inlet temperature of spray drying is 240-260 ℃, and the air outlet temperature is 105-125 ℃.
In conclusion, the invention has the advantages that: the method has the advantages of reasonable steps and convenient operation, and the prepared dried intestine membrane protein powder has good quality, high digestibility and good bioavailability, the salt content is less than 5 percent, the ash content is less than 10 percent, the color is bright yellow, the smell is fragrant, the taste is salty and fragrant, and the biological safety is effectively improved.
Detailed Description
Preparing intestinal membrane protein powder by using fresh intestinal residues after heparin sodium extraction according to a preparation method of the sheep small intestinal mucosa protein powder, wherein the method comprises the following steps:
(1) pretreatment of intestinal mucosa, namely collecting the intestinal mucosa by using fresh intestinal residues after heparin sodium extraction, adding a cleaning solution into a container to clean the intestinal mucosa, and filtering after cleaning to obtain a clean intestinal mucosa;
(2) performing enzymolysis, namely filling clean intestinal mucosa into an enzymolysis container, adding water into the enzymolysis container, then fully stirring by using a stirring device, adding protease into the enzymolysis container for enzymolysis, and performing enzyme deactivation after the enzymolysis is finished to obtain an enzymolysis solution;
(3) desalting, namely introducing the filtrate into a nanofiltration system for desalting to obtain desalted filtrate;
(4) concentrating, namely introducing the desalted filtrate into a double-effect reduced pressure concentrator for concentrating to obtain a concentrated solution;
(5) spray drying, namely adding terminal peptidase into the concentrated solution, and then carrying out spray drying to obtain the enzymolysis intestinal membrane protein powder.
Further, in step 1 of the method, the cleaning solution is an aqueous solution containing 2.5% NaCl.
Further, the method comprises the following steps: in step 2 of the method, the mass ratio of the water added into the enzymolysis container to the intestinal mucosa is 22-25: 100.
further, the method comprises the following steps: in the step 2 of the method, after water is added, the stirring device is used for fully stirring at the temperature of 40-50 ℃, the rotating speed of stirring is 300-600r/min, and the stirring time is 20-40 min.
Further, the protease added in step 2 of the method is alkaline protease (5000IU/g), and the mass ratio of the alkaline protease (5000IU/g) to the intestinal mucosa is 0.15: 100.
Further, in the method, in the step 2, the pH value is adjusted to 10 during enzymolysis, the temperature is adjusted to 52 ℃ during enzymolysis, and the temperature is kept for 4 hours at the temperature of 52 ℃.
Further, the enzyme deactivation in step 2 of the method is a high-temperature enzyme deactivation method.
Further, in the step 3 of the method, the filtrate is introduced into a nanofiltration system, and the nanofiltration operation conditions are that the operation pressure is 6bar, the feed liquid temperature is 55 ℃, and the pH of the feed liquid is 6.
Further, in the step 4 of the method, the desalted filtrate is introduced into a double-effect vacuum concentrator, the operation negative pressure is 0.4-0.5MPa, the temperature of the filtrate is heated to 75 ℃, and the filtrate is concentrated under the condition until the solid content is 45-48 wt%.
Further, the negative pressure of the spray drying in the step 5 of the method is 0.25-0.40MPa, the inlet air temperature of the spray drying is 240-.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210139043.2A CN114794311A (en) | 2022-02-15 | 2022-02-15 | Preparation method of sheep small intestine mucous membrane protein powder |
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| CN202210139043.2A CN114794311A (en) | 2022-02-15 | 2022-02-15 | Preparation method of sheep small intestine mucous membrane protein powder |
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| CN202210139043.2A Pending CN114794311A (en) | 2022-02-15 | 2022-02-15 | Preparation method of sheep small intestine mucous membrane protein powder |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117044847B (en) * | 2023-08-31 | 2024-03-26 | 广东京基智农科技有限公司 | California perch feed and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106520881A (en) * | 2016-12-10 | 2017-03-22 | 广州市嘉仁高新科技有限公司 | Method for enzymatic hydrolysis of dried porcine solubles from animal small intestines |
| CN108260707A (en) * | 2016-12-30 | 2018-07-10 | 上海杰隆生物制品股份有限公司 | A kind of preparation method of animal dried porcine saluble |
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2022
- 2022-02-15 CN CN202210139043.2A patent/CN114794311A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106520881A (en) * | 2016-12-10 | 2017-03-22 | 广州市嘉仁高新科技有限公司 | Method for enzymatic hydrolysis of dried porcine solubles from animal small intestines |
| CN108260707A (en) * | 2016-12-30 | 2018-07-10 | 上海杰隆生物制品股份有限公司 | A kind of preparation method of animal dried porcine saluble |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117044847B (en) * | 2023-08-31 | 2024-03-26 | 广东京基智农科技有限公司 | California perch feed and preparation method thereof |
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Application publication date: 20220729 |