CN114790484A - MNP (MNP protein) marker site of xanthomonas oryzae, primer composition, kit and application of MNP marker site - Google Patents
MNP (MNP protein) marker site of xanthomonas oryzae, primer composition, kit and application of MNP marker site Download PDFInfo
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Abstract
The invention discloses a MNP marker locus of xanthomonas oryzae, a primer composition, a kit and application thereof, wherein the MNP marker locus comprises marker loci of MNP-1-MNP-212 on a plurality of genomes of the xanthomonas oryzae. The primer is shown as SEQ ID NO. 1-SEQ ID NO. 424. The MNP marker loci have the advantages of large number, high polymorphism and strong species distinguishing capability, and meet the requirements of accurate, sensitive and culture-free detection of xanthomonas oryzae, pathological type and microspecies distinguishing, heterozygosity detection, genetic variation detection, authentication and construction of a DNA fingerprint database; the primers are not interfered with each other, the detection efficiency is high, the accuracy of the identification result is high, the result reproducibility is strong, the requirements for ensuring the reproducibility of the scientific research result and the requirements for large-scale field sample detection of the xanthomonas oryzae are met, and the method has great application value in the aspects of epidemiological detection and identification of the xanthomonas oryzae.
Description
Technical Field
The embodiment of the invention relates to the technical field of biology, in particular to an MNP (MNP) marker locus of xanthomonas oryzae, a primer composition, a kit and application thereof.
Background
Xanthomonas oryzae, including Xanthomonas oryzae pv. oryzae (Xoo) and Xanthomonas oryzae pv. oryzae (Xoc), causes bacterial blight and rice streak, respectively. Xanthomonas occurs in each rice area in the world, the influence on the rice yield of China is large, blighted grains and broken rice are more, the yield is reduced by 20-30%, the weight can reach 50-60%, and even the grains are not harvested. Indica rice is heavier than japonica rice, late rice is heavier than early rice, and the indica rice is incorporated into plant quarantine type disease management in China, America and other countries. Different pathological types of xanthomonas are in honey yellow on an artificial culture medium, the colony is the same in shape, water-insoluble yellow pigment is generated, the aerobiotic and respiratory metabolism are realized, but strains in different regions are different in pathogenicity, but the phenotype and the genotype are extremely similar, and the traditional methods such as thallus shape observation, serotype identification and the like are difficult to distinguish.
Xanthomonas causes huge damage to rice planting in China every year, different species of Xanthomonas have different damage degrees to rice, and different coping strategies in agricultural control are different, so that the first step of agricultural control is to determine which species infect rice.
In addition, Xanthomonas is also an important experimental model for studying the interaction between plants and pathogenic bacteria. The genetic inconsistency of strains used for scientific research may be caused by wrong labeling, pollution and genetic variation of the strains in experimental operation, and the variation causing the pathogenicity change of the strains can cause inconsistency of interaction results with a host, so that genetic variation detection and identity authentication are necessary to be carried out on the strains for experiments, and the reproducibility of the experiment results is ensured.
Established methods for identifying phytopathogenic microorganisms fall into two categories: (1) length-based genotyping methods such as Pulsed Field Gel Electrophoresis (PFGE) and multi-locus variable tandem repeat analysis (MLVA), and (2) sequence-based genotyping methods including multi-locus sequence typing (MLST) and whole genome sequencing SNPs (WGS-SNPs). The length-based genotyping method can only distinguish significant sequence length differences, but cannot distinguish subtle nucleotide changes. In contrast, the MLST method detects a small number of species-specific genomic sites by using PCR and sequencing techniques, is not suitable for mixed samples, requires isolation and culture procedures of the target strain, is very low-throughput and time-consuming and laborious. Metagenomic multisite sequence typing (MG-MLST) combines high throughput sequencing with MLST, bypassing the time-consuming isolation and culture procedure, however, also only a small number of loci (typically 4 to 8 loci) are detected, which limits the resolution of typing. WGS-SNP is another technique widely used for identification of cultured strains by analyzing SNP information of the whole genome. SNPs are bi-allelic molecular markers that are insufficient to capture the underlying multi-allelic forms in a population of microorganisms. The error rate estimates for SNP genotyping by WGS are high, which also makes it difficult to accurately detect variations with frequencies below the technical error rate. The existing methods are applicable to samples of cultured microorganisms, and in fact, many microorganisms are uncultured or difficult to culture. Metagenomics is a widely used method for the identification of uncultured samples. However, at the early stages of microbial infection, metagenomics requires ultra-deep sequencing to detect low abundance pathogenic microorganisms, which makes the cost unacceptable and it is difficult to distinguish taxa under the species.
One of the main objectives of plant protection is to accurately and finely identify pathogenic microorganisms in the early stage of disease, and therefore, the development of an accurate, sensitive and culture-free plant pathogen identification technology is a technical problem to be solved urgently.
Disclosure of Invention
The invention aims to provide a MNP marker locus, a primer composition and a kit of xanthomonas oryzae and application thereof, which can not only detect and finely classify xanthomonas oryzae of various cultured and non-cultured samples, but also monitor variation, realize the identification and identity authentication of small seed homozygosis and heterozygosis, and also perform genetic analysis on xanthomonas oryzae and have the effects of strong discrimination, high flux, high accuracy, no culture and high efficiency.
In order to achieve the purpose, the invention adopts the following technical scheme:
in a first aspect of the invention, a MNP marker site of Xanthomonas oryzae is provided, wherein the MNP marker site is a genome region which is screened on a Xanthomonas oryzae genome and has a plurality of nucleotide polymorphisms in Xanthomonas oryzae species, and the MNP marker site comprises marker sites MNP-1-MNP-212 on the Xanthomonas oryzae genome; the marker site sequences of the MNP-1-MNP-212 are shown as SEQ ID NO. 425-SEQ ID NO. 636.
In a second aspect of the invention, a PCR primer composition for detecting the MNP marker site is provided, and the PCR primer composition comprises 212 pairs of primers, wherein the nucleotide sequences of the 212 pairs of primers are shown as SEQ ID NO. 1-SEQ ID NO. 424.
In a third aspect of the invention, there is provided a detection kit for detecting said MNP marker site, said kit comprising said primer composition.
Further, the kit also comprises a PCR premix.
In the fourth aspect of the present invention, there is provided the use of said MNP marker site or said PCR primer composition or said detection kit for detecting xanthomonas oryzae in cultured and non-cultured samples.
In the fifth aspect of the invention, the MNP marker locus or the PCR primer composition or the detection kit is provided for the application in the detection and the authentication of the genetic variation of Xanthomonas oryzae.
In the sixth aspect of the invention, the MNP marker locus of the xanthomonas oryzae, the PCR primer composition or the detection kit is provided for the application of the homozygosity and heterozygosity detection of the xanthomonas oryzae strain.
In the seventh aspect of the invention, the application of the MNP marker site of the Xanthomonas oryzae or the PCR primer composition or the detection kit in the construction of the DNA fingerprint database of the Xanthomonas oryzae is provided.
In the above application, the specific application steps are as follows:
firstly, obtaining the total DNA of a sample to be detected; carrying out first round PCR amplification on the sample DNA to be detected by using the kit, wherein the cycle number is 18; after purifying the amplification product, adding a sample label and a second-generation sequencing joint based on second-round PCR amplification; purifying and quantifying the second round amplification product;
when a plurality of Xanthomonas oryzae samples are detected, the second round amplification products are equivalently mixed and then subjected to high-throughput sequencing;
and performing data quality control and data analysis on sequencing data of a sample to be detected, comparing a sequencing result with the Xanthomonas oryzae reference sequence, and acquiring the number of detected sites of the sample to be detected on the MNP sites, the number of sequencing sequences covering each MNP site and the MNP site genotype data.
When the kit and the method are used for identifying xanthomonas from cultured and non-cultured samples, the number of MNP loci detected in the sample to be detected and the genotype data of the MNP loci are obtained by the kit and the method; comparing the obtained genotype with a reference genome database to obtain a genome with the closest genetic distance; and obtaining the typing information of the Xanthomonas in the detected sample according to the obtained pathological type and microspecies information of the genome.
When the kit and the method are used for analyzing genetic variation among Xanthomonas oryzae samples, genotype data of the samples to be compared at the MNP sites are obtained by the kit and the method; analyzing the sample to be compared on the MNP locus to jointly detect the MNP locus; obtaining the number of MNP sites with differences in the main genotypes among the samples through genotype comparison, and calculating the proportion of the number of the MNP sites with differences in the common detection sites, namely the difference proportion; and determining the genetic variation condition among the samples through the difference ratio.
When the kit and the method are used for analyzing the internal genetic variation of the Xanthomonas oryzae sample, the genotype data of the sample to be detected at the MNP site is obtained by using the kit and the method, and whether a secondary genotype other than a main genotype is detected at the MNP site of the sample to be detected is judged through a statistical model. If the sample to be detected has a minor genotype at least one MNP site, determining that genetic variation exists in the sample to be detected; and judging whether the sample to be detected is homozygous or heterozygous according to whether genetic variation exists in the sample to be detected.
When the method is used for authenticating the Xanthomonas oryzae sample, the genotype data of the sample to be tested at the MNP site is obtained, the comparison with the main genotype of the standard strain at the MNP site is carried out, and whether the authentication is passed or not is judged based on the consistency degree of the genotypes of the sample to be tested and the standard strain and the technical reproduction rate of the method.
When the kit is used for constructing the DNA fingerprint database of the Xanthomonas oryzae, the genotype data of the Xanthomonas oryzae to be detected at the MNP site is obtained by using the kit and the method; and recording a database file to construct a DNA fingerprint database of the xanthomonas oryzae.
One or more technical solutions in the embodiments of the present invention have at least the following technical effects or advantages:
the invention provides a MNP marker locus of xanthomonas oryzae, a primer composition, a kit and application thereof, wherein the 212 MNP loci of xanthomonas oryzae and the primer composition thereof can be used for PCR amplification, and a second-generation sequencing platform is combined for sequencing an amplification product, so that the detection of the xanthomonas oryzae has the characteristics of high flux, high discrimination, high accuracy, high sensitivity, culture-free and the like, experiments prove that the 212 MNP marker loci, the primer composition and the kit provided by the invention not only can meet the identification and typing of the xanthomonas oryzae in a sample cultured in a laboratory and a sample from natural scab leaves which is not cultured, but also can carry out genetic variation analysis, homozygous and heterozygous analysis and sample authentication, thereby realizing the high-efficiency, accurate, sensitive and culture-free identification, genetic variation detection and DNA fingerprint database construction of the xanthomonas oryzae, therefore, the 212 MNP marker sites and the primer combination provided by the invention have great significance for accurate prevention and control of xanthomonas oryzae in agriculture and guarantee of genetic consistency of strains in experimental research.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts.
FIG. 1 shows MNP site screening and multiplex PCR primer design;
FIG. 2 shows the results of detection of Xanthomonas oryzae samples that were separately cultured and uncultured;
FIG. 3 shows the results of the authentication of laboratory-preserved Xanthomonas campestris.
Detailed Description
The embodiments of the present invention will be described in detail below with reference to specific embodiments and examples, and the advantages and various effects of the embodiments of the present invention will be more clearly apparent therefrom. It will be understood by those skilled in the art that the present embodiments and examples are illustrative of the present invention and are not to be construed as limiting the present invention.
Throughout the specification, unless otherwise specifically noted, terms used herein should be understood as having meanings as commonly used in the art. Accordingly, unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which embodiments of the invention belong. If there is a conflict, the present specification will control.
Unless otherwise specifically stated, various raw materials, reagents, instruments, equipment and the like used in the examples of the present invention are commercially available or can be prepared by an existing method.
In order to solve the technical problems, the general idea of the embodiment of the application is as follows:
the invention screens a set of Xanthomonas oryzae MNP marker loci with high polymorphism and high species discrimination by a marker screening rule (see embodiment 1 specifically) developed by the inventor of the application based on Xanthomonas oryzae reference genome and combined with main Xanthomonas oryzae sequencing data, wherein the MNP marker loci are genome regions screened on Xanthomonas oryzae genome and having a plurality of nucleotide polymorphisms in Xanthomonas oryzae strains, and comprise marker loci of MNP-1-MNP-212 on Xanthomonas oryzae genome.
Then, the invention designs a PCR primer composition for amplifying the MNP marker sites, wherein the PCR primer composition comprises 212 pairs of primers, and the nucleotide sequences of the 212 pairs of primers are shown as SEQ ID NO. 1-SEQ ID NO. 424. The primers are not conflicted with each other, can carry out efficient amplification through PCR, and has high identification accuracy and strong result reproducibility.
The PCR primer composition can be used for a detection kit for detecting the MNP marker locus. The primer and the detection kit are applied to the identification of xanthomonas oryzae, genetic variation detection, authentication, database construction and other related fields.
The MNP marker site, primer composition and kit of Xanthomonas oryzae and the application thereof according to the present application will be described in detail below with reference to examples, comparative examples and experimental data.
Example 1 screening of MNP marker sites of Xanthomonas oryzae and design of PCR amplification primers
S1, screening of MNP marker sites of xanthomonas oryzae
In the embodiment, the assembled genome sequences of 458 Xanthomonas disclosed in NCBI genome database are used as a reference genome library, Bwotiie 2 is used for acquiring SNP information among different genome groups, and then 125bp is used as a window, 5bp is used for walking, and candidate MNP sites are screened; and (3) screening the MNP sites with highest polymorphism and specificity of the xanthomonas according to the polymorphism of the candidate MNP sites among strains.
S2 design of PCR amplification primers
In this example, Ion torrent S5 sequencing platform of life Technology was used for sequencing, so the sequence information of the MNP marker sites obtained above was submitted to the online design webpage http:// ampliseq. com/protected/help/pipeline details of multiplex PCR primers of life Technology for primer design. According to the design of primers, primers are not interfered with each other, all the primers can be combined into a primer pool for PCR amplification, namely all the designed primers can be normally amplified in one amplification reaction, and finally 212 MNP sites and 212 pairs of primer compositions disclosed in the table 1 of the invention are screened out.
TABLE 1-212 Xanthomonas oryzae MNP marker loci and 212 pairs of detection primers
Example 2 evaluation of the Performance of the MNP marker, primer composition and kit for the identification of Xanthomonas oryzae
1. Sample testing
After the primer design in example 1 was completed, 23 monoclonal samples and 16 artificial mixtures of two monoclonal samples were tested, and the effect of primer design was evaluated based on the site detection rate. Specifically, DNA of a sample was extracted using the Shanghai worker microorganism genomic DNA extraction kit, and then the kit 2.0 (Ion AmpliSeq, English name) was constructed according to the amplicon library of the American life technology corporation TM Library Kit 2.0, cat # 4475345) containing 5-fold concentration of high fidelity DNA polymerase mix (5 x hifix), excess primer digestion reagent (FuPa), adapter reagent (Ion P1 adapter), switch reagent (switch solution) and DNA Ligase (Ligase). Separately purchased labeled reagents (Ion Xpress) for distinguishing different samples TM Barcode Adapters 1-16Kit, cat # 4471250) was added to the amplification product of each sample to distinguish the samples.
The constructed library is mixed with a plurality of libraries depending on the chip to be used. This example uses an ABI Ion530 chip, which can support a volume of 25ul, 60pM concentration of mixed library. Since the same amount of data was required for each library, an equal amount of mixing was performed in this example. Mixed library according to Ion 520 TM &530 TM Kit-Chef Kit procedures Chef procedures were performed on Ion Chef systems, and after completion of the procedure, the pooled library samples were spread evenly onto the chip. And performing single-ended 300bp high-throughput sequencing on the paved chip on an Ion S5 sequencing instrument, setting the sequencing depth to be 1000 times of coverage of each amplicon, and obtaining sequencing data of all samples.
2. Data analysis
Obtaining effective MNP sites of each microorganism strain to be detected: sequencing data is first controlled, qualified data is distributed to each sample according to a sample label, and then Bowtie2 (version 2.1.0) is used for distributing the sequencing data of each sample to each site. Sites with 1 sequencing sequence are detected sites, and sites more than 100 are effective MNP sites.
Obtaining candidate genotypes of each MNP locus, including a major genotype and a minor allele genotype;
the genotype represented by each sequenced fragment is then determined based on the sequence alignment information. The number of sequencing fragments supported by each genotype was counted. Genotypes with the most sequencing fragments supporting and the number exceeding 50% of the total site-covering sequencing fragments are major genotypes, the remainder being minor genotypes. The presence or absence of the inferior genotype reflects the homozygosity and heterozygosity of the detected races.
Determining the authenticity of the sub-allelic genotype:
the authenticity assessment of the sub-allelic genotypes of this example was performed as follows: the following strand-biased alleles were first excluded: (1) a strand preference greater than 10-fold, or a difference from the strand preference of the dominant allele greater than 5-fold; (2) fisher tests p-value < 0.05.
Genotypes without strand preference reuse statistical models for their authenticity. The statistical model first assumes that the candidate minor allele is the product of the major allele due to PCR or sequencing errors. Under this assumption, the number of reads supported by the candidate sub-allelic genotype (c) fits a two-fold distribution based on the depth of site sequencing and the amplicon sequencing error rate e (n), where n refers to the number of SNPs that differ between the candidate sub-allelic genotype and the major genotype. If the number of supported reads for the candidate allele exceeds the expectation, i.e., P (X ≧ c) is significantly low, the assumption that the allele is the dominant allele due to PCR or sequencing errors is overridden. When there are multiple candidate sub-alleles, the P-value of each candidate allele is multiply corrected, and candidate alleles with FDR < 0.5% are considered true sub-alleles.
Two parameters in the statistical model were evaluated:
amplicon sequencing error rate e (n) refers to the highest proportion of the number of reads at the site that carry the wrong alleles of n SNPs. This example obtained the frequency of the detected sub-allelic genotypes, i.e., the error rates of these genotypes, in the expected homozygous MNP sites (i.e., sites where the genotypes between the two parents were identical and all were homozygous) in the rice hybrids by calculating max (n-1) and e max (n≥2)。
The calculation method is as follows:
the number of the difference bases between the ith sub-allelic genotype and the major genotype is ni, the number of the supported reads is ci, and the error rate ei is the ratio of the number of the reads of the sub-allelic genotype ci to the total number of the reads of the site N, i.e. ei is ci/N;
calculation of e at different sequencing depths max (n) of (a). Low sequencing depthIn the following, the amplicon sequencing error rate is too high. To be able to detect low frequency variations, the sequencing depth of each site was set to at least 1000 x for high throughput sequencing. This example summarizes the error rates of all the minor alleles of 930 homozygous MNP loci in all 16 rice F1 samples. Depth of sequencing>＝1000*,e max (n-1) and e max (n is more than or equal to 2) is respectively 1.03 percent and 0.0994 percent.
TABLE 2 evaluation of amplicon sequencing error Rate at different sequencing depths
Inv was then calculated based on binom. inv under the probability guarantee of α ═ 99.9999%, e max (n-1) and e max (n.gtoreq.2) are respectively 1.03% and 0.0994%, the critical value of the number of reads of each sub-allelic genotype, and the authenticity judgment can be carried out only when the number of reads of the sub-allelic genotype exceeds the critical value.
TABLE 3 critical value for determination of sub-allelic genotypes at partial sequencing depth
3. The kit and the detection sensitivity evaluation of MNP site detection Xanthomonas
Sequencing data were analyzed for 22 monoclonal samples and 24 artificial mixture samples according to the parameters described above. The number of the detected sites is 203 on average, which indicates the high efficiency of the primer system. The true minor genotypes were not detected in the monoclonal samples developed from 6 single cells, whereas multiple expected minor genotypes were detected in all 24 artificial mixes prepared by proportionally mixing the single cells, with sample ratios as low as 1/1000 indicating the sensitivity of the developed MNP markers and kits for xanthomonas detection and authentication.
4. The kit and MNP marker detection accuracy and reproducibility evaluation of xanthomonas detection
Evaluating the accuracy and the reproducibility of the detection method, and selecting 12 small varieties from the evaluation method to perform the following steps: 1) technical repeats-3 technical repeats within the same batch, 2) sequencing batch-repeats-two sequencing performed by different people at different times. Technical repeats and sequencing batch-to-batch repeats are illustrated with the BJ-P1 sample in Table 4, BJ-P1 being the first sequencing batch, BJ-P1-rep1, BJ-P1-rep2 and BJ-P1-rep3 being the 3 technical repeats of the second sequencing batch. The results are shown in the table below, and the two strains with genotype locus differences can stably reappear between sequencing batches and technical repeats of the same sequencing batch, and the two strains with consistent genotypes can also stably reappear between sequencing batches and technical repeats of the same sequencing batch, which indicates that the reappearance rate Re of the main genotype of the MNP locus detected by the technology reaches 100%. Based on the low probability of occurrence of events with the same result but the same error result in two repetitions, the accuracy of detecting the major genotype of the MNP locus by the technique provided by the present invention is also considered to be 100%. Therefore, in this example, the threshold setting for the authentication of the strain to be tested is 100% of the similarity Si with the standard strain.
TABLE 4 100% reproducibility and 100% accuracy of the results of the sequencing batch and inter-technical repeat testing
Example 2 detection and authentication of 40 cultured Xanthomonas oryzae pv. oryzae (Xoo)
1. In this example, 10 pathological races of xanthomonas oryzae Xoo, which are most commonly used in BJ, WH, HN and IRRI 4 different laboratories, were detected and certified, specifically including:
step S201, acquiring an MNP fingerprint of a microorganism to be detected and authenticated by adopting the method in the first embodiment;
step S202, obtaining MNP fingerprint of the standard strain;
and S203, comparing the obtained MNP fingerprint of the strain to be detected with a reference genome of the species, so as to obtain the pathological type and the most similar race of the strain to be detected on taxonomy.
Step S204, comparing the MNP fingerprint of the microorganism to be authenticated with the MNP fingerprint of the standard strain to obtain main genotype and MNP site information, and obtaining the similarity Si of the microorganism strain to be authenticated and the standard strain, wherein the similarity Si is the proportion of MNP sites with the same main genotype of the to-be-authenticated strain and the standard strain in the common MNP site;
s205, comparing the similarity Si with the technical reproduction rate Re, and if the Si is more than or equal to the Re, judging that the to-be-authenticated microorganism passes the authentication; if Si is less than Re, the to-be-authenticated microorganism authentication is judged not to pass.
Specifically, the xanthomonas oryzae certification is to compare the MNP fingerprints of the microbial strains to be detected and the standard strains thereof, and the essence is to compare the MNP fingerprints of two samples. This example illustrates the effect of the kit and MNP marker on detection and identification of cultured microorganisms by comparing two identically named strains from 4 places, 60 sets of 10 races, and based on the difference in genotype between two samples.
(1) Detection of cultured Xanthomonas oryzae
Average number of sequenced fragments per microspecies 1,676,528 for 40 microspecies, and average number of sites 203, average sequenced fragment coverage per site 8245 (table 5) were detected. The genotypes of 40 strains and the genome sequence of 458 Xanthomonas oryzae strains are compared, and 39 strains are found to be Xoo pathological types, wherein one HN-P5 is Xoc pathological type and is closest to the BXOR1 race of Xoc pathological type, which indicates that the MNP marking method can finely identify the Xanthomonas oryzae and can distinguish the pathological type to the level of the race.
Table 5 shows the distribution map of the number of MNP-labeled detected loci of Xanthomonas oryzae
(2) Analysis of genetic variation among cultured xanthomonas oryzae strains and strain authentication
Two-by-two alignments based on major genotypes were first performed on 4 copies of the same race, with genetic similarity Se of group 43 being 100% and 23.8% -86.0% of group 17 in group 60 comparisons (table 6, fig. 3). Based on the method provided by the invention, the technical reproduction rate is 100%, and 17 strains with genetic similarity lower than 100% have genetic variation, and the authentication is judged to fail. The clustering analysis based on MNP locus genotype is carried out on 40 races, the clustering result can reflect the authentication condition of the laboratory-preserved Xanthomonas races which are separately cultured, and as shown in figure 2, the results show that 4 copies of the same race are not clustered together, but are clustered together with 4 copies of other races, which shows that the genetic difference between the copies of the same race is large and is larger than that between different races, and the research results generated by using the races have different risks of comparability and repeatability. The method provided by the invention can accurately and effectively carry out identity authentication on the cultured strain.
TABLE 6 fingerprint similarity of identically named races in four regions
Example 3 detection of genetic variation in Xanthomonas oryzae Strain and analysis of Homozygosity and heterozygosity
Genotype analysis for each locus detected a total of 254 true sub-allelic genotypes in 36 out of the 40 races detected, indicating that the 36 races were heterozygous. The number of minor alleles per race ranged from 1 to 37, with an average frequency of 2.8% minor alleles (as shown in Table 7). Of the 254 minor alleles, 84 were less than 0.9% frequent. 0.9% is the lower limit of the error rate of existing SNP analyses based on WGS, indicating that current methods based on SNPs are not identifiable for sub-allelic genotypes below 0.9%. This shows that the MNP labeling method has obvious advantages over traditional methods, and can detect genetic variation inside strains accurately and sensitively, and analyze homozygosity and heterozygosity of strains.
TABLE 7-40 location and frequency distribution of the detected minor alleles of genotypes
Example 4 detection of Xanthomonas oryzae on 4 uncultured specimens of naturally diseased macula leaves
In 2 consecutive years, rice leaves with similar rice leaf blight diseases are collected in the same paddy field to obtain 2 rice leaf samples, 2 commercial bacterial DNA extraction kits are respectively adopted for the 2 rice leaf samples to extract DNA of total bacteria in the leaves, and 4 DNA samples are obtained in total and are named as 2019_ kit1,2019_ kit2,2020_ kit1 and 2020_ kit2 respectively. And (4) carrying out MNP marking method detection on the 4 samples to obtain MNP fingerprint. Compared with reference genomes of 458 Xanthomonas oryzae, the main pathogenic bacteria of the samples in 2019 are PXO211 race with the pathological type of Xoo, and the main pathogenic bacteria in 2020 are L8 race with the pathological type of Xoc. The results of the clustering analysis performed on 40 Xanthomonas oryzae tested in example 3 were also consistent, with 2019 samples clustered with the Xoo pathotype race and 2020 samples clustered with Xoc (FIG. 2). The method can accurately detect and classify the xanthomonas oryzae in the sample obtained in nature under the condition of no culture.
Example 5 application of construction of Xanthomonas oryzae DNA fingerprint database
And inputting the MNP fingerprints of the 40 detected xanthomonas oryzae strains into a database file, wherein the MNP fingerprints with the same fingerprint spectrum are only input once to form a DNA fingerprint database of the xanthomonas oryzae. The genotype file of xanthomonas oryzae detected in any new sample is compared with the established MNP fingerprint database in genotype, and the MNP fingerprint spectrum of the sample with the difference of the main genotype is recorded into the established MNP fingerprint database, so that the database is continuously updated. Because the sequence information is recorded and is compatible with the existing sequencing-based method, the constructed database has the characteristics of sharability and co-construction.
Finally, it should also be noted that the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
While preferred embodiments of the present invention have been described, additional variations and modifications in those embodiments may occur to those skilled in the art once they learn of the basic inventive concepts. Therefore, it is intended that the appended claims be interpreted as including the preferred embodiment and all changes and modifications that fall within the true scope of the embodiments of the present invention.
It will be apparent to those skilled in the art that various modifications and variations can be made in the embodiments of the present invention without departing from the spirit or scope of the embodiments of the invention. Thus, if such modifications and variations of the embodiments of the present invention fall within the scope of the claims of the embodiments of the present invention and their equivalents, the embodiments of the present invention are also intended to encompass such modifications and variations.
Sequence listing
<110> university of Jianghan
<120> MNP (protein marker locus) marker site of xanthomonas oryzae, primer composition, kit and application of MNP marker site
<160> 636
<170> SIPOSequenceListing 1.0
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<400> 114
gaaaatgctg ctgatccagc aa 22
<210> 115
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 115
gagtaccgta agccgtgttc aa 22
<210> 116
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 116
ccagtgcgag atcatccagt tc 22
<210> 117
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 117
atcacgatca gcaccagcga aa 22
<210> 118
<211> 18
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 118
gctggcactg cagcatca 18
<210> 119
<211> 18
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 119
gcgcagctct tcgagcaa 18
<210> 120
<211> 19
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 120
ggcggtgttg cactatgtg 19
<210> 121
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 121
ctgtgtagtt gaccgaggca ta 22
<210> 122
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 122
caacttcgag cacaagcagt tc 22
<210> 123
<211> 19
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 123
atgagcaggg cttgcagat 19
<210> 124
<211> 19
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 124
cggccagata ctccagcaa 19
<210> 125
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 125
tcgtcgtttg ctggaagagt tt 22
<210> 126
<211> 23
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 126
aggatggtct tgttgagttt gct 23
<210> 127
<211> 25
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 127
tcgactgacc agttgatgaa tcatc 25
<210> 128
<211> 24
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 128
caacgttctt ttcttcgaca cgat 24
<210> 129
<211> 18
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 129
gcatcggctg caccaagt 18
<210> 130
<211> 18
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 130
ttcgcagcag gtgggact 18
<210> 131
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 131
<210> 132
<211> 21
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 132
gccggtgaag cgatacagaa a 21
<210> 133
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 133
<210> 134
<211> 21
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 134
cagaaggtcc tggcgatctt c 21
<210> 135
<211> 21
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 135
gcagaagacc tacaacggtg t 21
<210> 136
<211> 21
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 136
gccagccaac gcaatttgta g 21
<210> 137
<211> 18
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 137
gcacgcatgg ttgcaacg 18
<210> 138
<211> 19
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 138
aacgggtggt cgcggtaac 19
<210> 139
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 139
cgtcttccag tacctgggtg at 22
<210> 140
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 140
gcagcagcac aatgaaactc tc 22
<210> 141
<211> 19
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 141
gcatgtccgc atctccagt 19
<210> 142
<211> 19
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 142
acactggtgc atcggcaat 19
<210> 143
<211> 23
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 143
tatcaccatg catgccgtga taa 23
<210> 144
<211> 25
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 144
cggtgtgatg cattgttggt ttaac 25
<210> 145
<211> 18
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 145
gggcagacga atggtcga 18
<210> 146
<211> 18
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 146
ccgataccat ccgcagga 18
<210> 147
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 147
cgaccaagcc acccttcttc 20
<210> 148
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 148
<210> 149
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 149
gacgaattcc tctacggcaa ca 22
<210> 150
<211> 26
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 150
gatcagatca cttggagaga gagcta 26
<210> 151
<211> 27
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 151
tgatgattct gaaaactcgt catttgc 27
<210> 152
<211> 24
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 152
cgtcgcctat tttattctgg gcat 24
<210> 153
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 153
gcagcgaatt ttcgtgccat tt 22
<210> 154
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 154
ggtgtgatgt cgtgcagttc tt 22
<210> 155
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 155
<210> 156
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 156
cgcgcttaga acttcacatc ca 22
<210> 157
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 157
<210> 158
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 158
<210> 159
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 159
cggttttcgc catgtggatt tc 22
<210> 160
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 160
ggcccatacc gcattgaatt tc 22
<210> 161
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 161
ccttccaatt gctgacgttc at 22
<210> 162
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 162
acgtatcgaa aacaccgctc at 22
<210> 163
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 163
tcatcggcaa tcgctacatc at 22
<210> 164
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 164
cggcacctgt atctgttcga tg 22
<210> 165
<211> 19
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 165
gcgcagcaca ccaatgatg 19
<210> 166
<211> 19
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 166
ctggaacgcc agctacctt 19
<210> 167
<211> 18
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 167
agcaacctgg gcaagtcc 18
<210> 168
<211> 17
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 168
catggcgctg ctgcttg 17
<210> 169
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 169
gggtgctgcc gatatactca ac 22
<210> 170
<211> 23
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 170
ccagggagat atcggcaatc ttg 23
<210> 171
<211> 18
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 171
ggctgggagt tggtcgaa 18
<210> 172
<211> 26
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 172
gggtgaatcc gatctgaact ttttag 26
<210> 173
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 173
ccaccgcgag gacaaataga tt 22
<210> 174
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 174
gccgatagcc cattggtact ac 22
<210> 175
<211> 18
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 175
ccggcatgta ggtctgct 18
<210> 176
<211> 18
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 176
gaactggcca gcgcattg 18
<210> 177
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 177
<210> 178
<211> 21
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 178
gcgcattttc cagtccgatg a 21
<210> 179
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 179
cattggccga tttaaggcga at 22
<210> 180
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 180
gacgtgacgg cattagtaac ga 22
<210> 181
<211> 18
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 181
gcctgtccac gcgttttg 18
<210> 182
<211> 18
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 182
gcgctgtcct tgcacctt 18
<210> 183
<211> 17
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 183
gatggtttcc ggcagcg 17
<210> 184
<211> 17
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 184
tgctgatgcg cgtgcac 17
<210> 185
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 185
gaaacacacc ctgttcgaac tg 22
<210> 186
<211> 27
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 186
catcatgatc ggatagaaga aagcgaa 27
<210> 187
<211> 23
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 187
cccaaatcga tggaaaggtc gtt 23
<210> 188
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 188
cgtgaccggc acatgaaaat ag 22
<210> 189
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 189
cgaaccatct gttccagttg ct 22
<210> 190
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 190
cctgcagttt catcgccaca ta 22
<210> 191
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 191
gcgacctgtt cctgagattg ta 22
<210> 192
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 192
gcactgtcct ggtccatgta ac 22
<210> 193
<211> 23
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 193
gacaaagagc tgatcggtat cgt 23
<210> 194
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 194
tcgcgaatga tcttcttctg ca 22
<210> 195
<211> 19
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 195
tagcccagcg tacaggtct 19
<210> 196
<211> 24
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 196
tctcttctac gacctggaaa cctt 24
<210> 197
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 197
<210> 198
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 198
<210> 199
<211> 19
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 199
gggtcagctt gatgccgta 19
<210> 200
<211> 18
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 200
cgacgtggtg gtggtcaa 18
<210> 201
<211> 17
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 201
ggcttgcgag caatccg 17
<210> 202
<211> 17
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 202
ccgtttcggt gcgcaga 17
<210> 203
<211> 21
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 203
tgctactgtt tcatcggcaa c 21
<210> 204
<211> 18
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 204
accgtttgag gtggcgat 18
<210> 205
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 205
<210> 206
<211> 18
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 206
gacgcttgtg cgtcactt 18
<210> 207
<211> 18
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 207
ggcaccggac gtctatct 18
<210> 208
<211> 23
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 208
gccggtaaat cacgcatatt gtc 23
<210> 209
<211> 18
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 209
gaacgctacg cgctcaac 18
<210> 210
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 210
ccctggtata cggcttcgtc 20
<210> 211
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 211
cgctcaattc ttccggtgtc at 22
<210> 212
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 212
atctggagcc gaacgaaagc ct 22
<210> 213
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 213
ggccaagatc gtcaaggaga tc 22
<210> 214
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 214
gctcgtccag cacatacatc ac 22
<210> 215
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 215
<210> 216
<211> 21
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 216
tccatcaacg ggatccactc t 21
<210> 217
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 217
agctaggcat cagccagaaa ac 22
<210> 218
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 218
gcaggtctgc ttccagttga tc 22
<210> 219
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 219
<210> 220
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 220
<210> 221
<211> 24
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 221
agctatcaaa gatgtcctct agct 24
<210> 222
<211> 19
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 222
gacccgtgtt ctgagccaa 19
<210> 223
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 223
<210> 224
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 224
<210> 225
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 225
<210> 226
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 226
<210> 227
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 227
cgtttcagga acactggaag gt 22
<210> 228
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 228
gtttggtggc ccaaagttgt tt 22
<210> 229
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 229
<210> 230
<211> 21
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 230
ggcccaatcg aagaattcac c 21
<210> 231
<211> 23
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 231
tcgaatgtgt agttgaagcc gaa 23
<210> 232
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 232
cctgtgtctt accgctattc gt 22
<210> 233
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 233
<210> 234
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 234
<210> 235
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 235
<210> 236
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 236
<210> 237
<211> 18
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 237
gcgttggcgt tctggaat 18
<210> 238
<211> 18
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 238
gcagcgagtg gatgctgt 18
<210> 239
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 239
cgaggctgta attgaccgac tt 22
<210> 240
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 240
gctacgagca ttaccaggac tt 22
<210> 241
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 241
<210> 242
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 242
<210> 243
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 243
<210> 244
<211> 21
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 244
gctgatgatg ggcgtgtaca t 21
<210> 245
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 245
<210> 246
<211> 21
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 246
aaacagatag gcctgcggaa a 21
<210> 247
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 247
cgcatgggca ctcccattat ta 22
<210> 248
<211> 23
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 248
gacagcgttt gatctgcaag aag 23
<210> 249
<211> 18
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 249
cctgcgtgtg tcgcatca 18
<210> 250
<211> 18
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 250
gtccaggcgg tgaaggat 18
<210> 251
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 251
gcattgcagg aatacgtgca ag 22
<210> 252
<211> 24
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 252
cttatcagcc gcttgcaaca tatg 24
<210> 253
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 253
ctggtagcca cagggattga tg 22
<210> 254
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 254
gcgattacgt gtgcaagatc ga 22
<210> 255
<211> 17
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 255
tttaccttgc gctcgcc 17
<210> 256
<211> 17
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 256
catgtgctgc ggctgca 17
<210> 257
<211> 18
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 257
ctcgctgacc ctgtggat 18
<210> 258
<211> 19
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 258
gcgagcaact cgcggtaat 19
<210> 259
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 259
ttgcgacagt cacgggaaga aa 22
<210> 260
<211> 23
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 260
cgacgatcct tgcgttgtat ctt 23
<210> 261
<211> 18
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 261
caggtggcta cgactgca 18
<210> 262
<211> 18
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 262
agttgggacg cgacttcc 18
<210> 263
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 263
<210> 264
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 264
<210> 265
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 265
ggcgtgtcgt cgaacaattt tt 22
<210> 266
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 266
gcagtggagt gctaccaaga ac 22
<210> 267
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 267
<210> 268
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 268
<210> 269
<211> 19
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 269
ctgaccatgc ccaggatct 19
<210> 270
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 270
<210> 271
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 271
gctcgtcatc aatggcaaga ag 22
<210> 272
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 272
cgatgtggta cggcatgatc aa 22
<210> 273
<211> 23
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 273
caaattcact gcatggctac ctt 23
<210> 274
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 274
<210> 275
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 275
gccagacctg ctttgaactg at 22
<210> 276
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 276
tgtagtcgta ctcgccgatc tt 22
<210> 277
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 277
<210> 278
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 278
<210> 279
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 279
<210> 280
<211> 21
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 280
tagcttgtcc gcatgcttca t 21
<210> 281
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 281
<210> 282
<211> 23
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 282
gtgatgttgc ttagtgttca ggt 23
<210> 283
<211> 19
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 283
agcaatgggc agttgtgga 19
<210> 284
<211> 21
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 284
catcgatcgt acgtccacca t 21
<210> 285
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 285
cggtacaacc acacacaaca tg 22
<210> 286
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 286
<210> 287
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 287
cgtgatcgag aaactgcgtc aa 22
<210> 288
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 288
tgtgcttgtt gctcaccttc tt 22
<210> 289
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 289
<210> 290
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 290
<210> 291
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 291
<210> 292
<211> 21
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 292
gtactgacgc accaggaaga t 21
<210> 293
<211> 18
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 293
gcggcagcca tcgatgat 18
<210> 294
<211> 19
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 294
tggtagcgtt gctgctgat 19
<210> 295
<211> 18
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 295
ctgcgcgaac tggcagaa 18
<210> 296
<211> 19
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 296
gtcaacgcac tcaacgcca 19
<210> 297
<211> 18
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 297
gcccatcgga aagacgtc 18
<210> 298
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 298
<210> 299
<211> 18
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 299
ctggtgctgc tgctgttc 18
<210> 300
<211> 19
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 300
gcggcattca agtgccatg 19
<210> 301
<211> 18
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 301
gtaacgcgct gtcgcaat 18
<210> 302
<211> 19
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 302
gcgcttcaag caggcattc 19
<210> 303
<211> 25
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 303
aactggattc aagaatgtga gcgta 25
<210> 304
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 304
gcgattcatc acccggttca tt 22
<210> 305
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 305
ctgctgccgt tttgttcgta tt 22
<210> 306
<211> 23
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 306
gggcaaaccc ttctacctga atc 23
<210> 307
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 307
<210> 308
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 308
<210> 309
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 309
<210> 310
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 310
<210> 311
<211> 19
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 311
cgaagcggat ccgttcgta 19
<210> 312
<211> 19
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 312
ccgggtagcc gaatcgatg 19
<210> 313
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 313
<210> 314
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 314
ccaccggtgg gtatctgttc 20
<210> 315
<211> 21
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 315
gtggtgcaga cacgcagaca a 21
<210> 316
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 316
<210> 317
<211> 23
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 317
ggcaaagtcg ttcactgtat tgg 23
<210> 318
<211> 18
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 318
tcgctgatgg cactgcat 18
<210> 319
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 319
<210> 320
<211> 21
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 320
cgacatgtag tcaccggcaa t 21
<210> 321
<211> 18
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 321
gcagctggct gctgttga 18
<210> 322
<211> 19
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 322
cgagcaactc caccgcatt 19
<210> 323
<211> 24
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 323
cctcgtcctt cttgacattc atgt 24
<210> 324
<211> 29
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 324
tccaatttca atctggatat cgagttgtc 29
<210> 325
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 325
<210> 326
<211> 21
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 326
cccttcctct ggagtgctca t 21
<210> 327
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 327
ggtgccgcag caggataaat at 22
<210> 328
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 328
tggtgacgat ctggttcgag ta 22
<210> 329
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 329
gcaggcagtg acatcatcag tc 22
<210> 330
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 330
cgaggattcc ggctcgaaat ac 22
<210> 331
<211> 18
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 331
gtgccgtatg gcgacaat 18
<210> 332
<211> 18
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 332
caaggtgctc gaacgcat 18
<210> 333
<211> 19
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 333
gccaacgcat cgtcgatca 19
<210> 334
<211> 23
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 334
gacacgaagt cgtaatgctt gag 23
<210> 335
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 335
cgcgttcttc cagagtgtgt ac 22
<210> 336
<211> 23
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 336
gcaccggatt gcctttgata gat 23
<210> 337
<211> 18
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 337
gcgccttttc gatcgctt 18
<210> 338
<211> 19
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 338
tgctgcaggt gctgttctt 19
<210> 339
<211> 21
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 339
cgtcaaggac gatctgattg c 21
<210> 340
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 340
<210> 341
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 341
cgtattggga cgcggaaatc aa 22
<210> 342
<211> 23
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 342
gctggaatca cttcggcata cag 23
<210> 343
<211> 24
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 343
atgcatttca agagctcagt caac 24
<210> 344
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 344
gattgggata cagcgtggtg aa 22
<210> 345
<211> 19
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 345
gcggttgttg atcatcgct 19
<210> 346
<211> 17
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 346
gggcaccgat ccaactg 17
<210> 347
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 347
<210> 348
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 348
<210> 349
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 349
<210> 350
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 350
<210> 351
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 351
cgctggtcgt agatggtttt ga 22
<210> 352
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 352
accttcgaca acaccatcga ag 22
<210> 353
<211> 21
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 353
ggaggcgatc ttccagaaac c 21
<210> 354
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 354
<210> 355
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 355
gcgtgctggc agaaaacttc 20
<210> 356
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 356
<210> 357
<211> 23
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 357
gggaatgtag gtaatggcgt gat 23
<210> 358
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 358
ccgctatgtc gcggtgtatc 20
<210> 359
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 359
cggcgaccaa taacgacatt ct 22
<210> 360
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 360
tggcggtgac actccctata at 22
<210> 361
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 361
catttcgttg gcaagcaact ca 22
<210> 362
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 362
tgactgagaa ctggcacgta ga 22
<210> 363
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 363
<210> 364
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 364
gtgctgcatg cacaactttt tg 22
<210> 365
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 365
gctgaaggtg gtgccgtatt ac 22
<210> 366
<211> 23
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 366
ccattcagaa tgtggtcgac gtt 23
<210> 367
<211> 18
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 367
gcagtcgcgt tcgacaac 18
<210> 368
<211> 19
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 368
ggccgaaacg gacgtactt 19
<210> 369
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 369
gcggatgctt cttcttcgat ct 22
<210> 370
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 370
gcaatttgcc ctgatcgatc tc 22
<210> 371
<211> 21
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 371
cgtgcgagat cgtgttgcat a 21
<210> 372
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 372
<210> 373
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 373
gatgggagaa ggaatccacg tt 22
<210> 374
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 374
gccaggtctg cccattgatc 20
<210> 375
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 375
tttttggcct ggatcgactt ca 22
<210> 376
<211> 30
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 376
cggtttttca tttatcccta ttgatgttcc 30
<210> 377
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 377
tgttgaggaa caggttggca ta 22
<210> 378
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 378
gcttcgacat gtcgctgaat ct 22
<210> 379
<211> 18
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 379
gtcgcgttgc tccttgtc 18
<210> 380
<211> 21
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 380
ctgacccgca gcaacgagat c 21
<210> 381
<211> 18
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 381
gctgaccaag gcgcaatt 18
<210> 382
<211> 19
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 382
gccgaagatg cgcttcaac 19
<210> 383
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 383
acggtgcgtt gtttcgaaga ta 22
<210> 384
<211> 21
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 384
cgaggcaagc atctcgacat c 21
<210> 385
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 385
gcggctaaag aacgcaattt ct 22
<210> 386
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 386
gcatgctgca gaccttcatt tg 22
<210> 387
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 387
<210> 388
<211> 23
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 388
tggcgtaatc cagatgcttc ttc 23
<210> 389
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 389
<210> 390
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 390
ctgcgttgta gagcgaccaa ta 22
<210> 391
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 391
<210> 392
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 392
gcgttgcatc tcgtccgaat 20
<210> 393
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 393
gcgatgttgt tcgtcgaaca at 22
<210> 394
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 394
ccgatcatca gtacttcggc at 22
<210> 395
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 395
gcgctgatcg aataacacga tg 22
<210> 396
<211> 28
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 396
taacctgggt aaaatccaag tctttgag 28
<210> 397
<211> 17
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 397
gtacagacca gtggcgc 17
<210> 398
<211> 17
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 398
ccgctcacgc tggcaaa 17
<210> 399
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 399
gctcacgctg gacttctaca ag 22
<210> 400
<211> 23
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 400
caactccacg ttgatgctca aac 23
<210> 401
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 401
gggtgggctt gtcgaaatag at 22
<210> 402
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 402
cgacgtcgat acccaagtgt tg 22
<210> 403
<211> 18
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 403
cgacgtcgtc gcgcatat 18
<210> 404
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 404
catcaacgcc gatgccaatc 20
<210> 405
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 405
ggccagccca tagatgacaa ag 22
<210> 406
<211> 24
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 406
cactacgtgt atcccagcat cttc 24
<210> 407
<211> 21
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 407
atccgattcc accagcgaca a 21
<210> 408
<211> 21
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 408
agtggtgagc aggatccagt a 21
<210> 409
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 409
<210> 410
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 410
<210> 411
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 411
<210> 412
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 412
<210> 413
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 413
cggcgtagtc gaattgcttc tt 22
<210> 414
<211> 24
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 414
gattcagggt agccagacca tttt 24
<210> 415
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 415
<210> 416
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 416
gacgggcttc cgctagaatt 20
<210> 417
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 417
<210> 418
<211> 21
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 418
cgggatttcg cgacgaatga t 21
<210> 419
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 419
ccgtgtatcc gatcaaggtc aa 22
<210> 420
<211> 23
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 420
ccaagttcag ttcggaaggc ttt 23
<210> 421
<211> 19
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 421
aacctgcggc tgaggcatc 19
<210> 422
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 422
<210> 423
<211> 20
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 423
<210> 424
<211> 23
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 424
catcatggat ggcaacgtca aac 23
<210> 425
<211> 137
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 425
acgaaatgct tcaccgagga aatcacgtgg ctgccgtcca gaaactgcgg cgtgcccggt 60
gcgccctgga cgccttcgac catcttgccg gcgaagctgg ccaccacgtc cggcgactcg 120
gaatagcctt cgtaggt 137
<210> 426
<211> 170
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 426
gaagacgcct ttgtgattgg tgaatgccag cgcatcgcgc gtcaacgtaa acaccacgtc 60
ctggctctcg cccggctgca gcagcagctt gcgaaacccc ctgagttcgc gcaccgggcg 120
cacgcggctg gccacgcgat cgtgcacata caactgcacc acttcttcgc 170
<210> 427
<211> 132
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 427
cgactgctcg ctacgtgttg ttagccgctc ttagcgtgtg cgcctgttcg cgcacaccga 60
tgccagcgcc tgcagtcgcg ccgcaaccgg cgcctgctgc cgccgcgccg agcaagccga 120
acgtctccat cg 132
<210> 428
<211> 171
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 428
atcgccaacg cggtgaagaa ccacagctgc cagccgccgg catcgctcaa gaagctgaag 60
gccgcgccgg tgttataagt gcggtaccag ttccagaaac cgtcgatcac cggcaccggc 120
gtgtactcgg gcaggctgga cagcacccaa gccttgctcc actggtccag g 171
<210> 429
<211> 120
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 429
ccgttgccga agctttcttg caatcactgc aaacgtcgaa aaatcccttt gccgtcctgc 60
gcagcgtttc gcactccgcg acgcggctca ggtccagtcg gtcaggccgt cccggcgata 120
<210> 430
<211> 141
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 430
gtgcgttgaa cggtgcatcg gcgcggacga agcgcgccac gcgtgcgtag tcgcgtaagc 60
cggcattgac ctcggcgagc gccttggcga gcgcggcatc atcgctgtcg ggccggcgcg 120
gccatagcac cgcgacgttg t 141
<210> 431
<211> 174
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 431
agacacaaaa ccgtgcgaaa tcatctgcac catcgcgccc tgcagaccca gacgtgcagc 60
atcggtactg ccgtactcgc gcaccagggt gaaggcgatg aaggtgccca gcgtgacgaa 120
acccatgtgc gcgatggagg aatacgccac cagcttcttc atgtcgtcct gcac 174
<210> 432
<211> 154
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 432
ctgcagttca tcgaccagtg agggttgcct gacgctgcgc ggccagcgat ggccgttgca 60
gcgacaaacg ggaacaactg agcgctcacc gccacgcggg cgcggccagt cttcggaatc 120
ggcaggtgca actcgtacac tgcaattgct gcgc 154
<210> 433
<211> 192
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 433
caacgaagtg gaaacgctgt tcgtcgatga tcgcgaccgg gtgtgggttg gcgtcaatgg 60
ctcgccaccg agcatgctgg atgcgacgcg cacggtattt acgaggtttc ccaacgtcgg 120
tgcagcatgc gatgggcagg tctgggcgct ggcgcaggca caaggtgcga tctggatagg 180
aaccagtagc gg 192
<210> 434
<211> 273
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 434
ggaacgtcac cttcgtccta tctcagggac gcgttggccc acgcgtctcc tggagagcgg 60
acgtggctac gtctggattg gattgccgga atctctggtg cgttatgacc cgcgtacccg 120
acaaagcaag cgctggccgc tgtccacaga gcactatgtc gaggctgaca tggtgacacc 180
tgacctcatg gccttggatg cacgcagtca gctttggatt tccttgaata agatgggctt 240
ccagattcgt gatgaggaag gtcggcttat tcg 273
<210> 435
<211> 251
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 435
atcggccagg tccgagacat tgcgccgcag ggtgtgctcg gcatatgcgc cgccatcgat 60
gtaatgaaac aggaacggcg gcaagcgtgc ttgcgcggcg gcgcggtagt cggaagcggc 120
ggaaatgatc atcgaagaca ggacggcggc ggcggcggag ccaccgagtg taatgcgatc 180
ggtggggcag gtggagcgcc aagcccgctg agccggctgg cgagatcgcc gtcattcgtg 240
agagtgcctg c 251
<210> 436
<211> 215
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 436
gctgcttgga tggctgctcc tcacgctggc atctggctcc tgcggcagca ccctggccaa 60
tgacttcggc ggtgcggtgg cggtgacctc caggctgatc gaccgcggca tctccatcgc 120
acccaacaag gtcaccgtac aggcggccgg ttactggctg ccctcccctg gctggtcggc 180
ctctacctcg gtcgcgctgc agtcgaccga ctgga 215
<210> 437
<211> 201
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 437
ggtggataac gatgtgcacg atgggagtat caattccgct cttggccggc gcgctgcaga 60
tgcgcggtgt ccgccaacca atctggattg ccccagcggt tccattgcag ccaatgcagc 120
cagcgcgcga ctgatgtgct ggtgcctgat tggctgacgc aatcaccggc gcgtttgcgc 180
ttaagcaaga cgacctaggc g 201
<210> 438
<211> 264
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 438
ccgctaccca gattggaaaa catctggcgc aattgttggc ccatttcctc catgcccggc 60
ggggtcatga tgtccatgct ggcgttgacg gcgacttcca gttcgatctc gcgctcgtcc 120
agctctccgt tgcgcagcat gcggcggaac ttgatgcggg tgtcgttttc ctgcgacgac 180
ggctcgttgc gcgccgcttc cgggtcgaac ccaattccgg ttgcacgacg cggcaacagc 240
gcatccagaa tgcaatcttc ggca 264
<210> 439
<211> 217
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 439
cgcgacatgc cggtgataag ataggccaga ccgccgagca tcttcttcag gccttcgtcg 60
gtttccacgg tgacctgggg cccgaagccg ccgctggcca cattcgcgca ccagcgcggg 120
ccgtgttcgg cgtcgatgcg cagcaggtcg accggttgcg caacgcgttc ggcgatcagg 180
gtcagcgcgt ccagcggggt aatcgggacg ttggcgg 217
<210> 440
<211> 212
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 440
cgttggtgat ccacatcttg ctgccgttga gcacgtagcg gtcgccgcgc gcatcggcgc 60
gcagcttcat cgacaccacg tccgagccgg agccggcctc gctcatcgcc agtgcgccta 120
catgctcgcc gctgcatagt ttgggcaggt agcgctgttt ctgttcgggg ctgccgttct 180
tgcgcagttg attgaggcac aggttggaat gc 212
<210> 441
<211> 254
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 441
atcgaggtgg cgatgcaaca cgcggcggtg cgcgcttggc gtggcgcgcg tggcatcaaa 60
aaagcggccc cgggccgaca aggccagcgc gccgaatgca gcgcaagcag catgatcgaa 120
cccatcggcc gccatgcgtc atgcaactgc ccgctgtgca gttcgccgcg gttgtcggcg 180
ctggcgcgca gcaggtatcc aggcatcgcc ggcgtcgggt ggagcgcatg gacaactcac 240
caacagactg ccgc 254
<210> 442
<211> 259
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 442
atcgacgaca acggacagcg tatccgcgtg cagccgattg gcgaactgcg cgatctgcag 60
gaattgcgcg agttggtcct caatgccaag ggcgtacggc tgggcgatat cgccgaggtg 120
cggctcaaac cgatgcgcat gaattacggg cgccgcctgg atgggcgccc ggcgatcggc 180
ctggatgtct acaaagagcg cagcgccaat ctggtggacg tctccaaggc ggccttgaaa 240
gaagtcgaag acatccgcg 259
<210> 443
<211> 251
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 443
catgatcaag gtgtcgcagt tgagcatcgc gtggtagccg gaactgaaac cgatcaggcc 60
ggtcatgccg acatcgaacg ggttttccca ctccacgtat tccttaccgc gcagtgcatg 120
taccaccggt gcacccaagg tgtcggccaa ggacaccacc gcgtcatgcg caccggcgca 180
gccactgccg cataacaagg tcaccgcctt gctttgctgc aggagcgcgg ccaattgctg 240
cacatcctcc g 251
<210> 444
<211> 202
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 444
catcccgctc ttgacgttga attcgtcgcg cgcctggttc accgcctccg gcggcagcgt 60
atcgctgcac acgcgggtgc cgttctggtt ccagcaatac agcttcctgg ccgccgcctt 120
gtcccgcgca ctggccggtg gggccacggc gaatggtgca gcgacagccg ccgccacggc 180
ggccaataac gccaaacgtg tg 202
<210> 445
<211> 256
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 445
ctcaggcgct gcaaaaccag cagggcaaac tgttgttggg cggcagcggg cgcatcgccg 60
ccgccagcat cgacaaccag ggcggcaccc tgtacgcggc cggcacggcc ttgcaggtgc 120
aggtcgtcgg actgctggat aaccacgccg ccggcaccgt ctccagcgcc gggcagctga 180
gcgtgggcag tggatcgctc gacaacagcg ccggcacgct ggtggccggc accgatttga 240
acgtgaccac gacagc 256
<210> 446
<211> 273
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 446
attgactggc gtaacgagca tagaggccgg tatacactgc aataaagatg gcgcctatcc 60
aggctatgtt gtcgcgcaag gcaatctgcg cctcgcgtag cgacggatcc caagtgcgct 120
cgggatccac tacggccttc agcaccattg cgaatgtgaa aatcagcgca gtaaccagca 180
gggcacggag gaagatcaca ccgcctccgt tggggcgttc gcgcaatagc cactcgccgc 240
tgagccaagc aacaactttc aacactttgt cca 273
<210> 447
<211> 275
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 447
cgtcaggaaa tcggaccgaa tatgagcttc cgagaaagtc tcatgtatga ttctgatgcc 60
ttgttcaata gcaaacttgg ttattatcga ggcaatagag aacgttctga tcgcgactcg 120
ttaaatgaag aagcaattgc gaacaatgca tatgacgacg ccaatagagg tgccaaatat 180
aaattaggca acacacaacc tggtgacggc tggagatatc gagggcgagg attaaagcaa 240
ctaaccgggc gctcaaacta tcgaagcttt gcagc 275
<210> 448
<211> 275
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 448
ccaccaacca tgcatccttt gaaacctgat ctattgccgg cctagcattg aagatgcttt 60
gtagctcgaa aaaatcttcg ccagaataat ccacatacgg agagtcagcg taagaccatc 120
taacaaaatc aaaataatcc gccgcctcca aacccttatc cttttccctc tgcaaaacct 180
gctctgacca agcggaaatg aatggaggat gcccctcctc tgtagtaggt gcacctctag 240
ttggatcgtt ggatggtaac tttaggcttg gcagc 275
<210> 449
<211> 233
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 449
gtgggcgaag caagggtcat atgccgggcg ctcgccgcgc cccggtgctg ccaggcgtgg 60
tgcgtgagtc cgtccgcaag gccgacaagc accacatgac cggaaagccc acggccttga 120
tgcgacagct cgtacgcatt tgcgaggaag gtggccacat cctcgatccg ttcgctggta 180
gcggatcgac actcattgca gcacgcgacc agggttttgc ttggaccggc atc 233
<210> 450
<211> 275
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 450
agtatcacgt cccttgaaat acgaattgaa aaacgacttc gtaattccgg agtactcgct 60
ggtcatcgcc catagatctt tcggtctatg tgagatgcaa cgttcgacct cgaactcccc 120
tacaattttg cccacgggca tagtggcata gatgaccacg gtctttacac cgtgcgcttt 180
aggtagagcc ttccggaatt caaatcgctt tcgcccgtcg agaatctttt cagcatactc 240
aggcttgatt gacaataaga ctttcatcga tttcg 275
<210> 451
<211> 249
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 451
agcgacttga tcatcggctt cgggcgcttc gggcaggtcg ccagtcaatc gctgttggcg 60
cgcgacgtgg ctgtgaccat catcgacaac gacatcgaga agatgcaaag cgctgaagag 120
ttgggcttca agatctacta cggcgacggc cccggctgga cgtgttgcat gcctccggcg 180
cgcacagcgc acgcgcgatg tggtctgcgt cgacaaccag gaggcggcca atcgcatcgt 240
cgaactggc 249
<210> 452
<211> 256
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 452
cattggccac gtctactgga ttgagctcct tgcgtaaccc gggatcggcg gccagccgct 60
cggccatccc accggcagcg gcggcacaga cgggggtgtc aggccatttg ctcaaggcat 120
tcaacgcgtt ggccacgtcc agtgaattga aggtcttgcg taacaggcga tcgccgacca 180
ggcgccatgc caacaggcgg gcaacttccg cacagacctc ggtgcccggc catttactca 240
aggcgttcaa gacgtt 256
<210> 453
<211> 206
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 453
cagctggatg gtgtgcagat actgcctggc ttggtcgttg tactcggtga cgttgtagtc 60
attgatcatc aacttggtat gggggaaata cctgcgtgcc aggcggaatg cctcaagcac 120
ccaatcgacg ccagtggtgc cggtaccgcc cagtgcctgc atataattgc ccgtatcgct 180
gcggcgattg tccggctggt tgtgac 206
<210> 454
<211> 240
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 454
gcactgtggc gcacctatta cgcacatatc ttcaacccgg cgcggctcac ccagaccatg 60
atgcggcagg aaatgccgca gaaatattgg aagaacctgc ccgaagcagc gtcgctgccg 120
gaactgatcc gcgaagccgg tgtgcgcgtg cgcgagatgg ccgaacgcgc gcctgagcca 180
gtgcggccac gggtaccggc ggcgcctgcg ccacagccgg atacgacaac ccacagcctg 240
<210> 455
<211> 230
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 455
tccactcatc ccagtccagt tgcgacagtg ctgcgctatg cggatccggc acgccggcat 60
tgttgaccag gccgtcgatg cgtccgaaac gcttgagagc cgtggtgatc aggcgcgcgg 120
cctgcgcttc gcgcgcagca tcgcaacgca cgaaagcgct atgacgtggc agcgcccatt 180
ccctgcaggc aggccctgcc ggcgtcggca tccagatcgc cgatcatcac 230
<210> 456
<211> 275
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 456
caaaccataa tgcggtttgg acaagtcgat ccagacgcgg ccgaccggat tgttcgggcc 60
cggaggcaac gtggccttct gctcgccctt tttcgcgtcc cagaacaacg tggggttgta 120
cttgaacacc gggttgcgcg cgacgccgag gatcttccag cgtctgatcg gcagcgggtc 180
atgcttgcta ccggacgaca ccggaaattg cgcatacacc ttgccgtcgg cgtcgaatag 240
acgcagcgtg gaatcggatt tatccaccac caact 275
<210> 457
<211> 265
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 457
agatgcagtc gttctgtcag atacttttag cgggtgacgc ggacatccgt cgcgttattg 60
cagctgcgtc acgatctcgc gcgccatcgc gcccagcgcc aggatacggt ctgcgccgcc 120
gcgcttgatc gcttctttgg gcatgccgaa cacgatgctg gtctgttcgt cctgcgccac 180
cgtgcgtgcg ccggcctggc gcatttccag caggccggcg gcgccatcgt cgcccatgcc 240
ggtcatgatg atgcctagcg cattg 265
<210> 458
<211> 267
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 458
caacactgcc aagaaccatc tggttgcgca taaccaccgc ccgcccagcg acgacatcga 60
gatcagcgat gccgaacact tcgatggagc gacccggttg cgcgacaccg acaccccgga 120
gcgcgaattg atgcgacagg agctggaaca aacggtggat gcgcgcggta caagcactgc 180
cggaagaact tcgatcgggc atcaccctgc gcgaggtgaa gggctgagct atgaggacat 240
cgcgcgaaag atggattgcc cgatcgg 267
<210> 459
<211> 204
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 459
ggcatcggcg atcaacgtat ccaggtaatc gccgatcagc tcggccgacg atggcgcgcg 60
ggtaccgatg gaaaacgtca ggcatgcgtc ttcggccaca ccgtggtgcg ggatcagcgg 120
cggcagatac agcatgtcgc ccggacccag cacccagtgg tgggtgggtt tgaacgtgcg 180
cagcagcttg agctccacat cctc 204
<210> 460
<211> 221
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 460
cgaggttcac ccgtattacg tggtcaccga attcgctgca ggcgacgagg tgcaggaggc 60
ggccgcgatg gcggccttcg agacctgcat tgagcaggac tgggtcagcg atggcgtgat 120
cagccagagc gacgcacagg ccgcgcaact gtggcggcta cgcgagggca tcaccgaagc 180
ggtggcacgt tatacgccgt acaagaacga tgtgtcggtg c 221
<210> 461
<211> 273
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 461
ggtttgcgtc acgtcgttcg ccagtccccc gacagagggc agcgcacgtt gcgccgacac 60
cggctggccg acggccagca ttgcaagcca gcaccagcgc atgcccgcgc attttcgcca 120
tcgtccaccc caccatggtt gcttgttgca cgccatgcca cactaatcac gcctcgcaga 180
cgcaccgtga acgctttgca gcgcgcgcgg aagaaaacca ccttgccaga cgtatagctg 240
atgaacgatc ttgatcctgt ggtaaccgtc agc 273
<210> 462
<211> 203
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 462
tgcgtatcca tgacgcgaaa gttgctgatc ttcatgtttg cctgggtctc accattgccc 60
tgcggggcgc cgaacaatcg ctgacaggcg catgcgcgcc tggcggacgc acatcacaac 120
gtatcgcgat gcttgccgcg ccacggccgg taccaggtgc gaatcgctgc aatatcggcc 180
gccatgtcct tgccggtcca gaa 203
<210> 463
<211> 247
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 463
cggatcgaag cgatagtctg tcgacagatc gatcaccagc gtctgcggcg ttgctgcgtc 60
gatcgccgcg acgaatggtg cagcattgcc attgggcagc gccaggatca ctacgtcggc 120
cgcctttgcg gcgactgcgt tggcgtccag gctttcgtag cgcaactcag cctggtaggc 180
ctggttgtgc tcggccacgc gctgcccggc cagctcgcgc gacgagacga agaccaattg 240
cagatgc 247
<210> 464
<211> 270
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 464
gggccacgtc attgatgctg atgctcactg gaatcgttcc cgctccattt gcgcaccttg 60
acccaggcgc cgtatcgact ttgatgctaa cccgaaagca atacaacggt tttgtgcagt 120
gcacattgac gtaaattgtg ggaacgttcc cactacactt tccagcccga actcatccgg 180
agcccgcctt gtcctctccg tcgctcgccc ccgtcaaccc cgaccttgtg cgcctgcgcg 240
cgtctgtccg cgacaaggac gacgccatcg 270
<210> 465
<211> 257
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 465
caccgaactc acggctgata ggcctcgctg acgatgcgct tgagctcgcc gacttcaaac 60
aactccaggg tgatgtcgca gccgctaatc agctcgccat gaatgaacaa ttgcggaaag 120
gtcggccagt tggaatagcg cggtagattc gcacgcacct cgggttcttc cagcacgttg 180
atggtgcgca gctggtcggc accggcggcc accagggcct gcacggcgcg gctggaaaac 240
tcgcacatcg ggaattg 257
<210> 466
<211> 190
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 466
ggctcgtcat cggcgattag cacccgcact tgcgtggccg tcatggactc cccctggctg 60
cggtgacttt cccctcacgg cagcggcaat gtgatctcac aggcatagta gccttcagcc 120
cagctggccg caatccgtgc cccggcaccg aactggaatg ccagccggtg cgagatgctg 180
gcctgcgcat 190
<210> 467
<211> 240
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 467
cgggcttcac cttgatcgaa gtgttgctgg ccacgatgtt gttggtcggt gggctggcgt 60
tggcgtttgc caccttgcgc tcggccagtg cggtgagtca gcgcggcgaa gcgatcgcac 120
aacgcagcga gcgggtgcgt gcggtggaag aattcctgcg gcagcgcttg gctgcggcat 180
tgccgatcgc catggtcatc gatacgcaaa gccagcagcc gatcctgttc atcggcgagc 240
<210> 468
<211> 262
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 468
agccatccat caccacaatc aacatgtgcg gagagtgcag atgagcgaac gtttcaggac 60
cgaacacgac agcatgggcc aattgcaggt gcccgccgat gcgttgtggg gcgcgcagac 120
ccagcgtgca gtgaagaatt tcccggtgtc cgggcagccg atgccacgcg gcttcattcg 180
cgcgctgggc ctgatcaagg cggcggcggc cggcgtcaat gccgaacttg gattgctgcc 240
gaaatcgatc ggcaagacag tg 262
<210> 469
<211> 260
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 469
cgcccaattg cggatgattc cagcgcagta gcgcttccac gccatacagc gtgtgcggcg 60
cggcgctgtg catttgcggc tggtaatgca gttgcagctg atcgcggccg atggcctggc 120
gcagctccgc ttccagcgca acgcgttctt gcgccatgcg attcatgtcg gcgctgaaga 180
agcggaaacg cccgccgcct tctttcttgg cgcgattcat cgccagatcg gcgtggcgca 240
acaggatgtt gatctcgcgg 260
<210> 470
<211> 186
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 470
gtcggcatgg ctgagtacaa tggcctccag ccggcgcact ccgagcgcac gtagcgccgg 60
cagcaccacg cgctcgccgg cgtcgaaccc atcgcgcacc gccgggccgg tatcgaacag 120
cagcgcatgt cggtgcgtgc gcaccagcac cgccaagcct tgccctacat ccagcacctg 180
cacttc 186
<210> 471
<211> 206
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 471
gcgatccgct cactctttta caacaccgca tggacctcaa aagcggctat ccgttctggt 60
cgatccgcaa tggattgatg cgcgcttacc ctcgcctgga gcaagacacc cattgcgagg 120
tcgccatcgt cggtggcggc gtcaccgccg cgctgattgc ccacgaactc ctgcgccacg 180
gccatgaagt ggtggtgatc gaacag 206
<210> 472
<211> 233
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 472
gcagagttat ggccgagtgt tgaaagtctg ggcctgccgc ccgagctggc cgccacgcat 60
gtcgcctggc cggtgcatcc gctggtgtgg gagcggttgg agcaggaggg cttcgcgctg 120
ccagaaggtg cgctgtgcgc gccgaacgcc tggctggatg tgcgcccgac gctgtcggtg 180
cgcacgttgg tgccgctgca acatccgcaa ctgcatctca agctgccgat ccc 233
<210> 473
<211> 204
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 473
cagatagctg cggtccagat ggcgtccgtc ccagtgcagc aaccgcgggg tgcgcggcaa 60
gccatccagc tggcgcaagg ccagcgcctc gcgccgcgcc agccaccagg cgatgccacg 120
caacccccac ggcgccacgc tcaggtcgcg acgcacgaac acgtgctcgc tatcgcggat 180
caacaaaatg cgtccgaacg tgtc 204
<210> 474
<211> 256
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 474
ggatgacgca gtgtcatgca gcgcctgcag cgcggcatgc tccagcgcgc cgattcctgc 60
gttgcccagc aggtcgacat aggcctccag tttcggcgtc agaccccact ggtccagcat 120
gcgcaagtcg aacaggcgct tggcgagatt ggaaacgttg gcgctgcgcg cgaattcggg 180
atgggtatga gcgagcgcct gcagacggtc gccgatgtgg ccgccggaat cgtcgcaatc 240
ctcatagatc gctgtc 256
<210> 475
<211> 196
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 475
cgctggtagc tccagtgtgc catcgcccag ttcaccagta ccacgaaccg attgcggaag 60
ccgatcaaaa agtacacgtg cgcagctagc cagaaccacc aggcgacgat gccggacagc 120
ttgagcttgc ccacatgcac gatggcggcc atgcggccga tcgtggccag gttgccgtag 180
tcctggtagc ggaacg 196
<210> 476
<211> 259
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 476
ctacccagct gcagcaggaa ggcaagaacg acgaagcgct ggcgctgctg aaaaacatcg 60
agccgaaggc ggtcaaaaac tccgaactgc gcggcgcgct ggctttcgcc tacgacgcca 120
tgggccagac cgaagccgcg gcacgcgtgc tttcgaccgg gccgcaggac acccagacct 180
acggcttgcg cgcctcgatg ctggccaagg aaaaagaccg cagttcgttg gaagcgctgt 240
atacggagct gaaaagcaa 259
<210> 477
<211> 222
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 477
cggatgcaag gtatcgctca cctgcggcag cgggcgctgc tgttgcagcc gcgtgataaa 60
cggcgccaga gtgtcgacga tggaggggcg gacgttgtgc gcgctgttgg gcagtacgcg 120
cagcgccttc atgcccggca gcgcgttgta gaagaactgg gtgttgtccg gcaggaagaa 180
atcgtcgccg ctggcattga cgatgtactt gggcactgcc ag 222
<210> 478
<211> 269
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 478
cgctgccgtt ggtcttgagc cacttgccga tcgccagcag gatgccgcgc tcggtctccg 60
gaatcgaacc atcggcgcgc gggccgatgt tgagcatcag gttgccgttc ttggccacca 120
catcggccag catgtggatg atgaaggtgg gcgacttgta ggtgtcgttt tcgatatagc 180
cccatgaggc gttgctgacc gaggtgtcgg tttgccaatg cgtgggatgg atgccggtga 240
gctggccacg ctcgatatcc agcgtgcct 269
<210> 479
<211> 261
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 479
gcagcgcatg cttattcgaa tcgaacccgc ccgtttcgac gtccaccacc acgggcagat 60
agccgcgaaa gcggcgcgac atcggcaaaa aagagaaggc aggctgtgca tcgaccggtt 120
cgctcatccg catagcctag caggtcgcgc cccgggctca gcaatgaacg caaggctgca 180
ggtgttccgg atggaagcgc catgcatcgc cggggccgtc atcccgtgcg cgcggctggc 240
atttcagagt cggcatgcat c 261
<210> 480
<211> 205
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 480
gcgtgagagc tgcgaagaaa tcgcgctggg cgcacagcag gtgcacgcct tggggtatct 60
ggacccgttt ctgaccgtca gtggattccg ggtgacgccg gtggtggcgg tgatcgaccc 120
ggcgttcgtg gcggtcccgc agccggatga ggtggcggac gtcttcgaag taccgctggc 180
ctacttgatg gacccggaca atctg 205
<210> 481
<211> 214
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 481
gcggtggatg agaacaatca ggccacgcag gagcagcaat ccgatcatct gcccaaggtc 60
gatgtcgcca cgcagaatct gcgcacggcg tattggcaac aggcattgac cgcactggat 120
gccattgcgc aggcgcagct gtccgccgaa gatcaggtga gttaccaggt gtatcggcaa 180
cagttgcagg tgttgctgga tcagcagcat tttc 214
<210> 482
<211> 206
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 482
gagtaccgta agccgtgttc aagtcctgca atgttccggt atcgccattg aaggtggcgt 60
aattcaccca gtcgaacacg ttgaggatat ccgcacgcac attgaatttg acaccgccgc 120
cggtgtccca ttccttattc gcggcaatac tgaattcctt gtcgcccagc gtgccatccg 180
gcttgaactg gatgatctcg cactgg 206
<210> 483
<211> 273
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 483
atcacgatca gcaccagcga aatgaagctc aaattgtctt cgcggaacgc ttcgacggtc 60
tggatggccg cggaaggcag gcctgccacg ataccggcaa agatgatcag cgagacgccg 120
ttaccgatgc cgcgctcggt gacctgctcg ccaacccaca tcaagaagat ggtgccggca 180
gtcaacgcga tgaccgcaat gagcacgaaa cccaggcccg gcgcatacac caccggcgcg 240
ccaccgggcg cggtctgatg ctgcagtgcc agc 273
<210> 484
<211> 261
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 484
gcgcagctct tcgagcaact cgggaacggg cgcgaaattc atgccgtacc ccgcgtgccg 60
agcaggcgtt caacatagcg cgccaccaga tcgatttcca gattcaccgt cgcacccact 120
gcagtctgcg caaacgcggt attggcaatc gtatgcggaa tcaacgccac ttcaaggcct 180
tcttcgtcca cttcattgac ggtgaggctg acgccgtcta cgcagatcga acccttcttg 240
gccacatagt gcaacaccgc c 261
<210> 485
<211> 266
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 485
ctgtgtagtt gaccgaggca tatcccacgt ttctcggtgc gttgaccagg gtcttgccgt 60
cggtggcata ggtggtggtg ccaagcgcga catcgctctt catctggctg ttgttgtacg 120
cataggaggc ataaacagac cacaggtcgc tgaccttgta gctcaactcg gtattgacgc 180
cgcgcatctg cacgctgccc aactggtagt agaccggagc gtgggtcatc ggatcggcgc 240
cactgaactg cttgtgctcg aagttg 266
<210> 486
<211> 207
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 486
atgagcaggg cttgcagatc gagccggcgt tctggttgcg catggtgggc accggcgatg 60
tggcatgccg gctgttgctg ggtgaacgca tcggcgacgc cgctgccgag gccatgctgg 120
cgcacgcgca acgcttgtat gccgctgcgg tcgaacgtgg catcccgcag cggcccggca 180
tcatcgtgtt gctggagtat ctggccg 207
<210> 487
<211> 225
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 487
tcgtcgtttg ctggaagagt ttcgccgcga actcgcccag ggcgacgaga aataagggcg 60
cacccctagc tcttgaagat ttgattgggg tacaatatgc ggcttgctgg ccagccttgc 120
gtctggccct cctacgaacc tcgagcaatc gccgtgcggc gactgccggt ccaatatcac 180
cagacacgcg gtgcccacca tgagcaaact caacaagacc atcct 225
<210> 488
<211> 275
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 488
tcgactgacc agttgatgaa tcatcgcgcc agattcagct gcgctttctt gtgttgcccg 60
agggcgcgcg tatgggacct gcaaaatgtg acgctctacc gcttacatcg cggcgatacg 120
gcgcgtgtgt gtcgtaagtc cctcgatcct ctggttttac agcgttttac gcgagctcct 180
tacgcgagca cattgcactg acaccgtcgc tgggagcatt gcaaacttgt tgaacacacg 240
gatggagctg catcgtgtcg aagaaaagaa cgttg 275
<210> 489
<211> 237
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 489
gcatcggctg caccaagtgc atccaggcct gccccgtgga tgccatcgtc ggcggcgcca 60
agcacatgca tacggtgatc gcgccgctgt gcaccggctg cgagctgtgt ctgccagcct 120
gcccggtgga ttgcatccaa ttgcatccag ccggttgagc gcggcggtca gcgcgatgcc 180
gtgcccagtc gatgcctgcg tggtcatgga ttggcgcgca gtcccacctg ctgcgaa 237
<210> 490
<211> 197
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 490
ataaggccaa ccctgccaat ggcggcaaga cctgccgcac cggtctatgg cggtactcgc 60
gccaccccaa ttactttttt gagttcgttc actggtttgc ctacctggcg ctggccgtgg 120
gtgctggccc gtggccggtg gcgctgtgtg cgctcggccc ggtggtgatg ttcgtgtttc 180
tgtatcgctt caccggc 197
<210> 491
<211> 269
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 491
gcggtcagaa aatgttcgct gtcgaccatg cgcgtggcaa cgggctgcgc cgcgctccag 60
tgctgcagcg cttcgtccag ccgccactcg tccggagcct ggtagtccaa cctgtcggcc 120
cggtaatgcg ccatcaacgc ctcgaggttg tgcgggatcg actggcggtt gctggcattg 180
tcgatcgtca catagcgccc ccgatgcccg tcccgccgca aacctgcagc gaaggcgcgc 240
atggccgcga agatcgccag gaccttctg 269
<210> 492
<211> 198
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 492
gcagaagacc tacaacggtg tcgcaatcct gtcgcgctcg ccggcgctgg acgtgcagat 60
ggggatcccc gacttcgacg acgtgcagca gcgggtgatc gctgccacgg tcgatggcgt 120
gcgcatcatc aatctgtatg tggtcaacgg ccaggacgtg ggcaccgaca agtacgccta 180
caaattgcgt tggctggc 198
<210> 493
<211> 222
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 493
gcacgcatgg ttgcaacgca tgagtgccga actcgatgcc gaaacgcaac agctgcatcg 60
cgcctatgcc gacgaagccg actgcgccgc tgcgcaccgt gggctgacgc ggcaggcata 120
ccacgcacag gcgccggcct tgagcaatgc cttgcagcac cccgacgccg ccgagcgcgc 180
gtaccgctgc gaccagctag ccagttaccg cgaccacccg tt 222
<210> 494
<211> 219
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 494
cgtcttccag tacctgggtg atgcggcttt ccaccacggc cgctgaggca ccggtgtatt 60
ccacattcac cgagacgatg ggcgggtcga tcgccggcaa ctcgcgcagc gtgaggcgag 120
tgaacgacat cacccccagc acgatcagca gcaaactcat caccaccgcc atcaccgggc 180
gggtaatcga caggtcggag agtttcattg tgctgctgc 219
<210> 495
<211> 188
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 495
gcatgtccgc atctccagtg catggtatta cgcgaactag atgcgccagg cacggcaatg 60
cgcgcagcca gcggaaaaac atgctacata gctcacgctt cgaaccgagc ccacccctcc 120
ccggatgtcc acgcctgtgt cttccctgcc ccacgcgctc agcggcccga ttgccgatgc 180
accagtgt 188
<210> 496
<211> 263
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 496
tatcaccatg catgccgtga taaccagtca atcaggcagg cactcggcat gccgattcca 60
cgcagccagg acccgatcag cggcccgggc tggcaacaat aacgcgcatg cacacctccc 120
gggcggcatc gcccgcggca gccgtcggcg cgtaccgcgc gcatgccggt gcccggcgcg 180
ctgtcccact ggcaagaacc gccgcgttgg ttgccgggcc gcccaatccg ccccgccggt 240
taaaccaaca atgcatcaca ccg 263
<210> 497
<211> 209
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 497
gggcagacga atggtcgacc cgcaccgcaa ctggcatgac gcgcgtgagc gccccacgac 60
ctgccgcagc gacggtcttc ctcgcgcccc tctgtccttt tgcctgagag tttaaaaacg 120
cgtcaaggcg cgtggcccgc ctgcgtttcg tgccccttcg gcgccggcat cggccggtct 180
ctccagagtt gtcctgcgga tggtatcgg 209
<210> 498
<211> 258
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 498
cgaccaagcc acccttcttc cttgactccc gacctgcgat gaccaccatg caactcgatt 60
cggacggacg cctgcgccac ctgctcaccc tggaaggact gccgcgtgcc acgctgctgc 120
aattgctcga ccgcgccggc caaatccgcg atgcggcggt ggggcgcgtg ggcaagcgca 180
gcgtgctggc gggcaccgcg gtgtgcacgc tgttcttcga accctccacg cgcacgcgca 240
gttccttcca tctggcgg 258
<210> 499
<211> 275
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 499
gacgaattcc tctacggcaa caggctgcgt gcgcatgaaa tcttcacgct caatgctcgt 60
gaccgggctg ctcgtggtca ggccaggcgt cgagatacgg ctaccggtga cggtgatcgt 120
gtctagtgtt gctgcttcgt cggctgcctt ggtctgcgcg accgcagtcg ctgacagaac 180
ggcattaccg gtaccgagca ccaacgcgaa cacaaccgcg tcgcgcagtt tgttggactt 240
aaaattcatt agctctctct ccaagtgatc tgatc 275
<210> 500
<211> 275
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 500
tgatgattct gaaaactcgt catttgcgtt tcaatcccaa catccatggc gccccacgca 60
ccagcaccgc gcgcatcggc acgctcatcg cgatcgcgaa ggcgcttagc cccaacgccc 120
aggcgggcgt accgattgcg ctgcgtgcga tcaggcccgt ccgtgccacc acgctggtgg 180
ctaccaggaa gaccaacgta tgcgtgcata aaatcagcag cgtattggta ccgatccact 240
gcacccagcg catgcccaga ataaaatagg cgacg 275
<210> 501
<211> 230
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 501
gcagcgaatt ttcgtgccat ttcgtcgctg cgcgcgtcgt gcagcgcgag ccatcgatgc 60
cactgtcacg ctcggcgtga tatgtagtca gtacgcgtcc agcgcggcgc gatggtcgcg 120
tcacgcgtgc gccgcatcgg ctgcgctgca gttgcgcggt gaccacgcac ataccccgtc 180
ttgatgatcg attccatggg ccacgccaaa gaactgcacg acatcacacc 230
<210> 502
<211> 230
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 502
ccgtcgtact ggctgttcaa tgccggcgcg cggtacgact tcggccgcgt cggcggcgtg 60
gcggatgtcg ccctggcgct caacgtcagt aacctcaccg acaagcacta cttcgccagc 120
accggcacca acggctatgt cgcctccgat ccggatggct acaaccagac catggtggtt 180
ggcgccccgc ggcagtactt catgagtctg gatgtgaagt tctaagcgcg 230
<210> 503
<211> 273
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 503
ctgctcgaaa ccgaaggtga ggctgccgcg ccggccaagg ccgagaccaa ggccgcaccg 60
gccgctgccg cgcctgctgc cgcgccgggc agcaagccgc cggtgacgcc gtcgcatcgc 120
gccccggccg aaccggcgcc gtccaagccc gcaatggcca gcggcaagcc ggccgatatc 180
gcatgcaaga tggtggtgct cggcgccggc cccggtggct acacggccgc attccgcgcc 240
gccgatctgg gcctagacac ggtgctgatc gag 273
<210> 504
<211> 206
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 504
cggttttcgc catgtggatt tcaacgacgt tacggcgctg gaagcggcga tggccagcgg 60
cgatgtagcc gcggtgatgc tggagccgat tcagggcgag ggcggggtaa tgccggccgc 120
gccgggcttc ttgagccggg tacgcgcgct ctgcgatcag cacgatgcgc tgctggtgct 180
ggacgaaatt caatgcggta tgggcc 206
<210> 505
<211> 275
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 505
ccttccaatt gctgacgttc atgtagtggc ggctgaggcc ttcctcagtc tgctcttcga 60
ctctgggggg tgggcctgcc atttcgctca cccgtttcag gaaatggcga tccctgcttt 120
ccctcagcat tttcttcaac accggaatct ggccgaaggt ggtagcgccg ggcgggcgct 180
tttctggaag ataggacggt gcaccttcca gaaagggatt tctttcctga tcaatgacag 240
cgctaagcat ggcatgagcg gtgttttcga tacgt 275
<210> 506
<211> 245
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 506
tcatcggcaa tcgctacatc atggtcggca tcggtgtggg tcaggcgcat caaccggcgg 60
ccatcgaaat ccacgctgta cagctgccga tagtacgggt ctttacccgc atccatcccg 120
ctggcggtgt accagatgcg gcgctgcata tcgtccacac gcagtacatc gcgtaccacc 180
cacggcccgc gggtgagctg gcgtttcagc tggccgttac gcccatcgaa cagatacagg 240
tgccg 245
<210> 507
<211> 211
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 507
gcgcagcaca ccaatgatgt gctgcagata cgggctgtga ccgccttgcg gcaagaacac 60
cgcctcgccc tcgcgacggc tgatgcgcgg atgctccagg tcgacatgaa tcaccggttc 120
ttgcaccaac gcgccgtcaa tgtgctgctc ctggaaaccg atcaagaacg gccccttggc 180
gacgatgccc ggaaggtagc tggcgttcca g 211
<210> 508
<211> 274
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 508
agcaacctgg gcaagtcctg cgacagcgca cacccgatcg agccagcgat gaacaacgcc 60
agcccgccca ggatcacccg ctcggcccca cgcatcggag agcggcccag gcgcgatgct 120
catcaggcca taggccagca gatagacgct gacggtctgc tggatcgcca cctggtccac 180
cgccaggctg cggctgagct gcgagaacgc cgggaagatg gggtcgatcg aaaacgggcc 240
gaacatcgcc agcccggcaa gcagcagcgc catg 274
<210> 509
<211> 233
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 509
gggtgctgcc gatatactca acgctctcgt cgaggggcgc tgcgaccgtt ggggcggact 60
gatccgccat ccgcaccaca tgtgcggaga cacacggcca ggctcgacca gcattcaccg 120
ttcaacggcg cccggaactg cagacattgc gtctgcctac cggagccatt gcatgaacgc 180
tgtcacaaag attacccccc acgccgatta caagattgcc gatatctccc tgg 233
<210> 510
<211> 240
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 510
ggctgggagt tggtcgaaca ggcgctgcag cgcaccgata cgcggtcctg gcgggtggtc 60
gcctagctgg cccactgctt gcgttcctga acgggctgct gggatatcga tggtttcgag 120
ggtttccagc agcgggcgcg tccgccacag cccacgccca tcatggcttt gagcactgac 180
gatattcatt tcgctgaatg ccgttacctc gaagctaaaa agttcagatc ggattcaccc 240
<210> 511
<211> 243
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 511
ccaccgcgag gacaaataga ttgaggcgca taggtatggt cttcggcagt gaaaaggggc 60
tgggcgtcct gccatggcgg tcgcaatgaa tgtcgtcagc aggcgcgcag ggtccgaacg 120
acggcagcac agaaatggtg acggtgtccg cgttaaacgg aattaacggc cggcggcggc 180
gcggctgaag tgtaagtcga cgttcagctg ctggatatgg ggtagtacca atgggctatc 240
ggc 243
<210> 512
<211> 245
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 512
ccggcatgta ggtctgctcc atgccgcctc gcgcaagggc catggtcgcc tggcaactgc 60
gacgtcatct ttacgtcgcg caaggccgca gttgcgcatc agccattgga catttccggc 120
agcggctcgg cgcattgtcg gcaatagcgt gcgtcggctg cgtggccttg cagcccgcag 180
cggacgcagt tgcggatctc gcgtttgccg gtgtggccgc tttcgcgcaa tgcgctggcc 240
agttc 245
<210> 513
<211> 241
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 513
gtagatcggc ttgccgaaga tttcggtggt gtgcttggcc ggcgtggcgg tcaggccgat 60
acggcaggca tcgaagtagt cgagcacgcg gcggtattgc gacaggtagt gcgcgtggtc 120
gcgcacggcc agctcgccgt cggtcatttc ctggtcgagc gtgtaaccac ggtgggcctc 180
gtcgacgacg atacagtcga aggtgccgat cggtggcggg tcatcggact ggaaaatgcg 240
c 241
<210> 514
<211> 238
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 514
cattggccga tttaaggcga atcgtcgaac gacagcacca tgccgggctt caggatggcg 60
cccttatcta ggccattgtt ggcgagcagg gtcgtcacgg tgacgccata gcggcgtgcg 120
attgcccagg ccgattcgcc attgcgcacg gtgtgcgtgc gggttggcga ggtcgacgct 180
ttgctggctg ccgtggcaac gacagtcggc gcggaatcgt tactaatgcc gtcacgtc 238
<210> 515
<211> 213
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 515
gcctgtccac gcgttttgca gcggcatgcc atgcctcttg aaggttcatc catgacgatc 60
tcaaagttcg catccagcag gttgcctcga cacggcgtgc tgctgttgtc catcagcgga 120
ttctgcgcgc cagcggcgga cgcacagacc agtgaacagc gcaccgatgc cgaggccacg 180
acgctggaca cactgaaggt gcaaggacag cgc 213
<210> 516
<211> 275
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 516
gatggtttcc ggcagcgccg cggcgtgcac cagatccatg ccggcaggaa tcggcagcac 60
gtgacgcgca tcgacggcgg cgtattgcgc atagccgccg ccgcccagca gtgcgcacac 120
gcgctcgccg actttccagc gaccagccgg ctccacgatc tcgccggcga tttccaatcc 180
cagcgtttcc ggtgcgccag gcggcggcgg gtagtgcccg gcgcgttgca gcagatcggg 240
gcgattgata ccggcggcgt gcacgcgcat cagca 275
<210> 517
<211> 275
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 517
gaaacacacc ctgttcgaac tgcagacggt ggactggcac acccagacgc cgatcagcgg 60
cgagcgcctg atcgcgcaga tccaccgcct gcagtcgcag ggcgtgcgcc attttgcgtg 120
gtatccggat gacttcatcg gcaacaggcc gtcgacggag gacgcacgcg cggcgatgtc 180
cgcacacaac ttcccttacc cggacaagtg acatggacac gtccgcatgg ctgtacggcc 240
tgttccagtt cgctttcttc tatccgatca tgatg 275
<210> 518
<211> 202
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 518
cccaaatcga tggaaaggtc gttggagaac atgccgcgga gtttcttgaa catcgaggga 60
gtcgttcctg ggtaagtcgc gtgcccattg ccagactggc gaaaatttgg gcaaaaaacg 120
aaggcgggta gcctagcaat cccccccggt acgggcaagg aaaaatcggt gaatgcgcat 180
ctattttcat gtgccggtca cg 202
<210> 519
<211> 246
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 519
cgaaccatct gttccagttg ctggcaatga ttgcgatgga accgccggcg gcatttacga 60
cggaagcaat gcatcgccgt cgcggcgaag tcatcgaagc ggtgcgcccg atcaaacccg 120
aagacgtggt acgtgggcaa tacgcctccg gtgcagtcag ccgcagcgca gtaccgggct 180
atcgcgaaga agacaccgtg ccggaggatt cggaaaccga aacctatgtg gcgatgaaac 240
tgcagg 246
<210> 520
<211> 254
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 520
gcgacctgtt cctgagattg taccggcttg gccggcctgc tgatcggcaa gacgctgggc 60
gtgggcagcg atgattggtc gcgctgattg ttcgccgcca gcgcgctggc ggtgcagagc 120
gaagcggtgg gcccgccggt gacctccacc cgttcgcgcg ccgatcaggc cggcgggatg 180
tggcgcattc cgctgcagca ccgggtgggg agcggcatcg tgtattccag ccgttacatg 240
gaccaggaca gtgc 254
<210> 521
<211> 275
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 521
gacaaagagc tgatcggtat cgttgccaag atcggcgtgg atttcatcgc cgtgtcgttc 60
tgtcgcaatg cgcaggacat gcaagacgcc cgtcagatcg ccaagcagca cagctgcgac 120
gcgcagctgg tgtcgaagat cgagcgcacc gaagcgatcg aaaacttggt cgagatcgtc 180
gaagcctccg atgtggtgat ggtggcgcgt ggcgaccttg gcgttgaaat cggcgatgcc 240
gaattgcccg ggctgcagaa gaagatcatt cgcga 275
<210> 522
<211> 273
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 522
tagcccagcg tacaggtctg cggccggctc atctgttcgg cgatgcgcgc gaacgcctcg 60
gcctcggtga cgccttcgcg cagcgcgtgc tgcggcgtga taccggtcac catggtggcg 120
tagggtgagg gcagcagatc gtcggccggc tgcacaaaga agctgatcgg ctcttcgatc 180
acgcgcagtt gcgcgtcggt ccgcaccgcg gcgaactgtg cgatgcgggt gcggcgcggg 240
tcctggccga aggtttccag gtcgtagaag aga 273
<210> 523
<211> 268
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 523
cgcatcggag accttctctt cgccttccgg ggcacgcacg cgcaccacgt attcgcgttc 60
gacctcgtag gaggggtgca tcatcgcgtt ggccagctca ccgtcggtgg tcaatagcaa 120
caggccggtg gtattgatgt ccaggcggcc gatggcgatc cagcgcgagc ccttcagcgc 180
gggcagcgac tcgaacacag tcggacggcc ttctggatct tcgcgcgtgg tcacttcgcc 240
ttcgggcttg ttgtagatca gcacgcgc 268
<210> 524
<211> 275
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 524
gggtcagctt gatgccgtag tccggcacca gatccacgaa ggggtcgtga cgcggcggca 60
aagcggcgtg caccgcctcc atcacgctct ggtagtcctg tgggccggac accgccagta 120
cctgcgggta cgcctcacga atctgctccg ggcgcttgcc caggcagccg gtgacgatga 180
ccttgccgtt ggcgctcatc gcctcgccga tcgcgtccag cgactcggtc actgccgaat 240
cgatgaagcc gcaggtgttg accaccacca cgtcg 275
<210> 525
<211> 275
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 525
ggcttgcgag caatccgggc gtgcacgtct tcccagcgtg gccacgccgc tgggtctgca 60
ggacgcggcg caggccagcg acacgcacgc gctacggctg accctggacc cgcagggcga 120
gcatcgcttg ggcacgttgg catcgagcat cccgcaacac attgtcgtgg cgatcgggcc 180
ggaaggtggc tggtcgccac gcgatcgcgc caccctcgcc gaggccggtt tcagcggcct 240
gcagctaggg ccgcgcattc tgcgcaccga aacgg 275
<210> 526
<211> 265
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 526
tgctactgtt tcatcggcaa cggaaccggg gacggggttg ggcaaccgtc ctttgcagct 60
gctggcagac acgcaaagag gagcgcaatt accgttgcag cagtgatttg ggcacaggtt 120
gcagggccga tgcccccccc ccccccctgg cgagcggcga catcgctgca gccaatcggc 180
tcgatgcgaa caaacaatga agcaatgatc gcaccggccg cctcggtaaa tctccccccc 240
ccggcgcatc gccacctcaa acggt 265
<210> 527
<211> 220
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 527
gtcggcgaaa tggccgagaa cgtgttccgc gtcggcctgg tccgcgtcga gcgtgcggtc 60
aaggagcgtc tgtcgatggc ggagtcggaa ggtctgaccc cgcaggaatt gatcaacgcc 120
aagccggtgg ctgcagcgat caaggaattc ttcggctcct cgcagctgtc gcggttcatg 180
gaccagaaca acccgctgtc ggaagtgacg cacaagcgtc 220
<210> 528
<211> 219
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 528
ggcaccggac gtctatctgc tggcggcacg cacagccgca gtagcgccgg agtgttgtct 60
ggtcatcgaa gacagcccca ccggtgcggc tgcggccgtt gcggcaggca tgcaggtact 120
ggggtttacc ggtgcgagcc acattccacc ggggcatggc gacaccttgc gccgcatcgg 180
tgtgctggag atgttcgaca atatgcgtga tttaccggc 219
<210> 529
<211> 266
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 529
gaacgctacg cgctcaaccg cttcgagcag cgcgtgctgg cgcagaaccc caacgccagc 60
gacgacggca atcccgccac gatgccgctg atgaccgaca aggacggggc gcaactacgc 120
gaaagcgccg gcgtggatgg atgggtcgtc aagaacatgc tgggcaccgc gacccggcgc 180
gaggccaacg gccccgggca ctaccgccac gtgctcgacc gtgaccacga aatgctgcag 240
ttcgtggacg aagccgtata ccaggg 266
<210> 530
<211> 231
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 530
cgctcaattc ttccggtgtc atccacaacg cgcgcaccac gccgacgtcc aggccgcgat 60
cggggtggtg cgtcagggca tcggcaacga aggcaaagcg caaaaagcac tgcccgttgg 120
gcgcaaccca ttggtaggta ccgataaact gggtcaggcg cacatcccag ccggtctcct 180
ccagggtttc gcgcacggcc gcctccagca ggctttcgtt cggctccaga t 231
<210> 531
<211> 176
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 531
ggccaagatc gtcaaggaga tcggcgagcg gctgggcttc ctggtcgatg tgggcctgga 60
ctacctcacc ctggagcgta aggccgacac cttgtccggc ggcgaagcgc aacgcattcg 120
cttggccagc cagatcgggg ccggcctggt cggggtgatg tatgtgctgg acgagc 176
<210> 532
<211> 248
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 532
gtcaaggcgc tggaggaata cggcatcggc cgtccctcca cctacgcctc gatcatccag 60
accctgcagt tccgcaagta cgtcgagatg gaaggccgca gcttccgccc caccgacgtc 120
ggccgtgcgg tgtccaagtt cctgtcaggg catttcaccc gctacgtgga ttacgacttc 180
accgccaagc tcgaagacga cctggatgcg gtctcgcgtg gcgaggcaga gtggatcccg 240
ttgatgga 248
<210> 533
<211> 260
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 533
agctaggcat cagccagaaa accggccaac gcatgggcca tatgagcatc gacggccctg 60
agggaacgat cgccgcattt gcgccgttga acgtggcgcg caaaatcttc atccatctca 120
acaccaccaa tcccgtgctc aacacccagt cgcctgagtt cgccaccgcg cgtgccagcg 180
gttgggaagt tgcccacgac ggcctggaga tcgctctgtg agtgcgctgc tcagccccga 240
tcaactggaa gcagacctgc 260
<210> 534
<211> 216
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 534
ccgcatgcgc attcttctac aacacgatcc cggcggtaac gcgccgctcc gctacgtcca 60
gctcaccctg caaccggatc tgttcggcgg ctgggagctt ctgcgcgaaa caggcgagat 120
cggtggacgc acacaactgc gacgcgacca gtacctgcaa caggatgaag cggaccgcgc 180
cttcgacaag gcgcgcgaga tccaactcaa acgcgg 216
<210> 535
<211> 275
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 535
agctatcaaa gatgtcctct agctgggcgt aattgcatga cactgcagcg atgcagcctg 60
caatccaggc gcctgcgctc acatgcttcc acgagaccgc cagtccattg agcaacagcc 120
aatgctcgaa gaataagggc tgcgtccgac cgttgccttc acggaagggg tgaaccatgt 180
tcagttcgcc atagagcttt gcgaccaacc gcatctgcgc ggagcgaggc agcgtggtca 240
gatcggcagc gtccagttgg ctcagaacac gggtc 275
<210> 536
<211> 255
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 536
agaagaccgt ctgcgcaaaa cgcctttgcc aagcccgcct ggggcggcag tggtctatca 60
actgccgatt tcctcatcgg caagccttga aaccccgctc ggcaaccgca tctgcacggt 120
atcgtcggcg ttgccggcca ccagttcaga ggatttcccc atgcggatgg acaagctcac 180
ctcgcggttc cagcaggcgc tggccgacgc gcagtcgctg gctgtgggcc gcgaccacaa 240
catcatcgag ccggt 255
<210> 537
<211> 238
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 537
gtcgagcgcg atcacccaat aatcggccca caccagcggt aaccagccca gccattctgg 60
cgcaaagcgc acttgcaact gccccggctg gccctcgacc gggcgcgcca cgccctcggc 120
ctgggtcagg ctgccatcgg cgatgcggca gccgttgcgc acgccgacca ggccatcgtc 180
gcgcaaggtg tagctggcgg tgatgtcgct gcggcatttt ttctggaacg acaccggc 238
<210> 538
<211> 208
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 538
cgtttcagga acactggaag gtgtttgtgg gctggaccgt gattggccta ttgatctact 60
tcggctacgg catccaccac agccgcctcg cgaaacaggc ctaaccttct ctcaagaccg 120
gcgcgcgacg ccggccttct tgttcaatga tcgcgcttag cgcaaagccg gaaccgcttc 180
atgttcaaac aactttgggc caccaaac 208
<210> 539
<211> 255
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 539
gtgaccatca gcgacctgat gggcgcggtg gtcggccgtt tgcaggcggt ggaaaacacc 60
gacgaagacg cgctggtgat cacgcgcgcc gacggctcgc tgctgatcga cggctcgctg 120
ccggtggaag aactccgcga agtgctgggc accgacttgc cggacgccga cgaaggcgat 180
taccacacgc ttgcgggcat gtgcatttat tacttcgggc gtattccgca ggccggtgaa 240
ttcttcgatt gggcc 255
<210> 540
<211> 275
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 540
tcgaatgtgt agttgaagcc gaagcgtggc tgccacacat cgttaccatc gatcgtagcg 60
tcattacgca cgccgaatgc gtcgaatgcg gtctgattga actgcgggct gttcggaacc 120
agctgtttat cataacgcag accgaaggtc aacgtcagat tactgttgac cgcccaagtg 180
tcctgcacaa acaggccaag attacgcaag ccccaattcg ctgcgatgtc atcgatgttc 240
ctggtattcg aggacgaata gcggtaagac acagg 275
<210> 541
<211> 242
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 541
ggcaccacga aatgctcaaa ggccacatcg tccagatcgg catcgccatc gggcgcttgc 60
gcggcctcaa gcgctgccca gcccggattg gcctgctcga gcaacagttg aaattcggtg 120
cgtggcatgc ggcgctcgca gcccatgcag cgcaccacat ccagccgccc atgcagatcg 180
atcaccgcct ggctgccagc ggcctgatgc aggcgatcca cgttctgggt gagcagcact 240
tc 242
<210> 542
<211> 220
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 542
ccgagtcgct caaccgtatt cctggcgtgc agctggcgcg cgacgtcaac ggcgaaggcc 60
tcaacatcgc catccgcggc ctgggcacca gcttcacaaa gaccacgctc aacggcgcca 120
gcatcgccac cgcctcgatc ggcctgaatg cgcagaacca gaaccgcgaa gtcgatctga 180
atctgtttcc gaccgaattc tttacccagc tcaccgtgag 220
<210> 543
<211> 235
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 543
gcgttggcgt tctggaattc ggccaccagc gtggtcaggt attcctcggc ggtctggcct 60
tcgtcggcgg ccaggtcgtc gatcatctgc tgcagctggg tcgaatattc cagcgagatg 120
gacttgcctt cggcttgctg caggttggcc aggtgcttga gcatggccag catcggcacc 180
gggttgtcgg cgctgcccat ggtctggccg ccgatcgaca gcatccactc gctgc 235
<210> 544
<211> 270
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 544
cgaggctgta attgaccgac ttttccggtg tcagatcggt ggcgcccagt ccacgcgcga 60
tgggattgtt gaccgacagc aagcggccct gggtcaggcg accgtcggcg tcgtaaccgg 120
tggaggttgc ttcgtaaccg atctggctga gcgagggtgc acgcacgttg ttggagatgg 180
cgccacgcag cgcaaaggcc ggggcgaacg cgtaacgtgc ggccagcttg ccggtccact 240
ggctgccgaa gtcctggtaa tgctcgtagc 270
<210> 545
<211> 221
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 545
cgcaaaggac gcagtttcag cgagcatatg cgtcgccagt tcggcatcga tggcgtcaag 60
gccgccctgc tctacgaaca tctcggcacg caggcgggcg acgatattgc gttggccgca 120
cgcacgctca agcagttgcc gctgcgcttg ctgcggacgc gcccgctcgc cgaagcgatc 180
agttcggcag gcggcgtgcg gcttgaagcg ctggacgatc a 221
<210> 546
<211> 265
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 546
gatggtgccg atcttgagga aggtggacag gtaatcgcgc ggtgccagca gcagccacac 60
cggcagcacc gaagcgacga agccatagcc gatcaacatc caggtgattt gcgtggcggt 120
gaaggtgaaa gccgggcccc aggtcgggtc cgccgccacc ttgccgccca gccagatcgc 180
gcccagcagc aggatcaacc ccaccaccga gatctcgccg atcttgccga cgcggatgta 240
gcgcatgtac acgcccatca tcagc 265
<210> 547
<211> 249
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 547
ccgcgtgtcc acatccatat cgacgacggt cgtcgctggt tgagggcaca agggggggcg 60
cgctacgatg cgatcatggt aaacagtacg ttccactggc gttctggtgc aacgacgctg 120
ctctcgcagg agttctttcg cctggttggc gcgcatctgt ccgattcagg cgtcgcgttg 180
gtgaatgcca ccgggtctcc agaggtcctc aagacggcag cttcggagtt tccgcaggcc 240
tatctgttt 249
<210> 548
<211> 221
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 548
cgcatgggca ctcccattat taacctctgg tgggagcagg tcggcggggc atcgaattag 60
cgcgtttcga cctgcaccct caacaggaag cgggaggccc tggcgcgaca tatccagatc 120
gatgtcgact gcgcagtaat ggttgccagc atctaaaaga tgccgccctg ccgcaaaaag 180
aaggccgcca acggcagcct tcttgcagat caaacgctgt c 221
<210> 549
<211> 220
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 549
cctgcgtgtg tcgcatcaac ccatcaatgt cgttctcgag taatgcctgc cgttcgccgg 60
ccagcgcatc gctgagacgt tgcaggaact cgttcacgtt caccctttca ggcggggatc 120
aacccgccag ttgctgattc agatccatca tgcggctggc gatggcatcc gggttgatgg 180
aatagctgcc gttctgcaat gcatccttca ccgcctggac 220
<210> 550
<211> 189
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 550
gcattgcagg aatacgtgca agatctctgg agcgaaattc gcgatgcatt gcgcaacgac 60
ctggcgcgcg agcagtcgtc gatggccgcg cacatggaac gttccatgca atcgctcggc 120
caggccttgt cgcaagaccc ctcgctacgc gaagcactca atcaacatat gttgcaagcg 180
gctgataag 189
<210> 551
<211> 221
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 551
ctggtagcca cagggattga tgcggtggaa cggctccaga tccatgtcca cgttcaacga 60
caacccgtgg aaagtacagc cacggcgcac gcggatgccg agcgcagcga tcttggcccc 120
acccacataa acgccggggg cgccgtcgcg acgctcggcc acgatgttcc attcgtccag 180
catgtcgatc agtgcctgct cgatcttgca cacgtaatcg c 221
<210> 552
<211> 240
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 552
tttaccttgc gctcgccggg cgggcgctgg ttccaatcgg ccgaacgcgc cgacattcgt 60
gaggagcttg ccgaagacat cgcgctggga accttgttgc cgctgctgat ggcgctgcca 120
ttgatggggc tgctgatctg ggccgtggtg aattgggcca tgcgcgcgct ggtgcgtgtc 180
tcctatgaga tcggcgagcg cgaccccaag cgcttgcagc cgctgcagcc gcagcacatg 240
<210> 553
<211> 231
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 553
ctcgctgacc ctgtggatgg ccgagcgcca tccgggtgcc agcatcacgg cggtctccaa 60
ctcacgcccg cagcgcgcgc acatcctgga gcagtgccgc gtgcgtgggc tgagcaacgt 120
gcaggtgatc actgccgacg tcaacgcgct ggcattgccg ccgggcagct ttgatcgcgt 180
ggtgtcggtg gagatgttcg agcacatgcg caattaccgc gagttgctcg c 231
<210> 554
<211> 189
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 554
ttgcgacagt cacgggaaga aaaccgatcc cccaagaaaa caggaaaggg gcggcgatat 60
caaccagcgc ggacagcaag agcacgagcg acgcgcgacg cctgatgtcc aggacccggc 120
caacgcacag acgacgcccc agctgcgctc ctaccgcgat acccagaaga tacaacgcaa 180
ggatcgtcg 189
<210> 555
<211> 275
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 555
caggtggcta cgactgcacg gcagcacggt ggcgtcgcgc agatgcacgc gcgcatcgcc 60
ggaatcgacc ggttcgatcg cctgcacttg gcccagattg accagatagc tgcgatggat 120
gtgcactaac acggccgggt ccagtttggc ctcgatcgcg gccatggtgc tgcgcaacgg 180
gtaatcgtga ccgcgcagat gcaggttgac gtcattgccc gcggcctgga tccactcgat 240
atcggcggtg gcgaccagga agtcgcgtcc caact 275
<210> 556
<211> 224
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 556
cgctgattcg ggcattcaat cggcaccgct gcgtgctgcc atacccgtgc cgatcatgcg 60
ttcattcggc ggtgatccag acctcaacaa gatccagaga gcacaccacg tcatggcaat 120
ggatgcgtgc cttcgatgac agcgcactcc acatcgtcca gcacccggcc cacctgctcg 180
gccagtagct ccagacaaaa cgccaactcg tccatgcaga tggc 224
<210> 557
<211> 240
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 557
ggcgtgtcgt cgaacaattt ttccaacaga ctgggttcga gtcccttcat tgaccgctcc 60
tacgtgacgt cgtccttgcg ctttgctgcc gactgggcgc gaccagcgga tggcgcggcc 120
ccgctgtgcg tcggtcctgt gtctgcgcgc ggcgcggaaa tgcgatggcg cgcgaaggcg 180
ccatcgcaaa acaaacatca aacggtgtag ttgggaatgt tcttggtagc actccactgc 240
<210> 558
<211> 262
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 558
cggcgtcaga atttcgatgc gggtattcgg cgaactggtc cggatcgccg agatgcagtc 60
gacgaaatgt tgcgcgccac cgtcgcgcag atcgtcgcgg tccacgctgg tcaccaccac 120
gtatttcagg cccatgtcgg ccacggtggc ggccaggctg gccggctcgt ttgcgtcagg 180
cggcttcggc cggccatgcg ccacatcgca gaacgagcag cgccgtgtgc acacctcgcc 240
caagatcatg aaggtcgcgg tg 262
<210> 559
<211> 261
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 559
ctgaccatgc ccaggatctc gccggtgcgc gggtccattg cgatggccgc gccttcgtgc 60
tcgccgaatg ccgccaccat tgcgcgctgc agatcggcgt ctaccgacag gcgcaggtcc 120
gcacccgatt gcggcgcgac ccggccaacg gtgcggatcg cccgcccctg cacgttggtt 180
tcgacctgct cgtaaccgac ctgcccgcgc agatcctgct cgtaatagcg ctccagcccg 240
gatttgccca tgtgggtcag c 261
<210> 560
<211> 209
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 560
gctcgtcatc aatggcaaga agaccgtctt gcatctgatc ccgtccggca tcctgcgcga 60
cgacgcgctg tgcctgatcg gcaatggcgt ggtgatctcc ccggccgcgt tgatcaaaga 120
gatcagcgag ctggaagacg ctggcgtgga agtgcgctcg cgcctgaaga tttccccggc 180
cgcgccgttg atcatgccgt accacatcg 209
<210> 561
<211> 239
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 561
caaattcact gcatggctac cttcaggcag ctgctgcgcc agaccgtgca catgcgcgat 60
gaaggtggcc agatcgaccg actgccccgc gcgccaggcc agcggccgat cgagcgcacc 120
aacagccatc ggatgggaaa tagcggaagc aggctggctg gacatgaaaa ccgtcggtcg 180
caagagcacg ctagcttggc cgccacccgc gctgctggca agtcgggaag ttccctctg 239
<210> 562
<211> 204
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 562
gccagacctg ctttgaactg atctcgcgcc cgctcaatgc cagcggtcgg ccggaccgtg 60
atgcagtgga tgccagcgac ggtcgcttcc tggcctgccg ctcgggcttc tacgatccgg 120
cggtgttcga acctggccgc gaagtgacct tcatcggcaa gatcgaaggc tacgaaacca 180
ccaagatcgg cgagtacgac taca 204
<210> 563
<211> 194
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 563
gcagtgacgg cgtacttgta gcgcgggcca gcttccacgc gcggcagaaa cagttcccag 60
aagccgccga tacgctggcg catcgggtgc agccgcacgt cccagccatt gaagtcacca 120
accacggcca cgcgttgcgc atgcggtgcc cacactgcaa agcgcacgcc gggcacttcg 180
ccacattgca catg 194
<210> 564
<211> 261
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 564
gtcccgcaac tgatcacgat tcgcgttcct tcatgccctg cgccaacgcg ttagagtgcg 60
gtcggcttac tgtctcggag acatcgcatg aagggcgata ccaaagtcat cgagtacctc 120
aacaaggtgc tctacaacga actcactgcg atcaaccagt acttcctgca cgccaagatg 180
ctgaagaact ggggcctgaa agaactggcc gaacatgagt acaaggaatc catcgacgaa 240
atgaagcatg cggacaagct a 261
<210> 565
<211> 261
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 565
cgcggtttca tccggataca ccggcagttg caatgcaggt ttgccgcgcc aactgtccgg 60
cgcccacgct gcggcggcgg gatcggaagc gggagtaggg gcggaaagat tcatgaacgg 120
actcctaggc agtctgcgat gttggcagag ccggaccgtt acgcaaagca cctgcatgct 180
aggcgcaccg gattgccacc gcagcgcccg cattggcact agcgcgcaag cgcctgcgac 240
ctgaacacta agcaacatca c 261
<210> 566
<211> 274
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 566
agcaatgggc agttgtggag ccggcgcgcg gccgtagacc gtgaccgtct gcgtcgtgcc 60
gttaccggtg ccggacagcg tgtccacccc ggtgacggtg ccccaccgtg cactgcggcc 120
agcgttgctg tagagctcgt aggtaatgta atcggcgctg ctggcgtggc gcatgcgccg 180
gacgttgccg gtggcgttgc tgccgttgtc cagcccgata ttccaggcgg tacggttggt 240
gcaggccagg ctgatggtgg acgtacgatc gatg 274
<210> 567
<211> 202
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 567
cggtacaacc acacacaaca tggcgaccgg caggaacagg cccacatgca gcaacagcaa 60
cggcacccga tacaggtacc ggagcaaacg caacggagcg ccagcgtcgc gcgtggcgtc 120
cggggatgac tcgctcattc gacgaacgga accggtaacg gggggggaag gaagcgcgac 180
cattgtgaca gcaacgtgga cc 202
<210> 568
<211> 230
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 568
cgtgatcgag aaactgcgtc aacgcgttgg cgtcgaggta gtacttgaaa tagtagatgt 60
acatcccacc tttcatggcg agattgatga agacacgatg gtgagcgcca gcatcaccag 120
ccagggtttg ttgcggacca gatcggtcac gtcctgacgc acgctgttgc gttgttcgct 180
gatcggcagc acgcgctcgc gcgtggtgaa gaaggtgagc aacaagcaca 230
<210> 569
<211> 236
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 569
gactggcggt atctcggaat gttcgtgccg tacgtcaata cccattagtg cgcccacggg 60
aaacacccac gggcgcaatg gcgtcgcggt tgcaggggga gcggcttgga agctggaacg 120
gtctgcaagg gcgtgcgaac tgggcgtgaa acgggggggc gcgatggtga cctatgacgg 180
tgggcgagac cgccacggct gcggcgattc cgtgcgtata cgcgaaggca caacgt 236
<210> 570
<211> 225
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 570
tcgttgctgg tcaacaccat ggccggttat gcgttcgcca agctgcagtt cgtcggccgc 60
gagtgcatct tcaagatcct gatggcggca ttggtgattc cggcgcaggt ggcgatgctg 120
ccgctgttcc tgctgatgaa gcagttgcac ctggtcaaca acatcggcgg ggtggttgtg 180
ccggcgttgg ccacggtgtt cggcatcttc ctggtgcgtc agtac 225
<210> 571
<211> 275
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 571
gcggcagcca tcgatgatga cgccggcatc gcgaatcgcc ttgcccagat gggcgcgcag 60
accggtgtgc acggcgaatg caatgccagg gtccagcgcc aaggcatgcg catggcgctc 120
gttcatggcc tgcaccacca gtacgtacgg gtcgtcgtgt tcttcccaca ggtatttagc 180
caccgggatc ttcagcaccg catccagcga gccttcttcg aatgcggggc agatcaatac 240
cgggtcgccg tccagcatca gcagcaacgc tacca 275
<210> 572
<211> 273
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 572
ctgcgcgaac tggcagaagc acgcaagtcg gccgcgctgg ccgctgcacg cgacagcgag 60
ccgcaggcct gacggcgacc ggatgaccat ccaactcagc gtcaacgtca acaagatcgc 120
ggtgctgcgc aattcgcgcg gcggcaccga tccggatgtg atgcaggccg cacgcacctg 180
cattgccgcc ggtgcgcacg gcatcaccgt gcatccgcgc ccggaccagc ggcatatccg 240
tgccggcgac gtgctggcgt tgagtgcgtt gac 273
<210> 573
<211> 275
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 573
gcccatcgga aagacgtcca gcaggccgcc gcggacggcg aaatcgcccg ggtccatcac 60
ctgcgggacg ttgcggtagc cggcgctttc cagccggcgt ttttccgcac ccagatccag 120
ccgctggccg acgataaggt cgaaactgcc gccaacgatg tagctcagag gggccagctg 180
ctgcagcaag gtctgcaccg gcacgatcac cacgcccttg ctcaggccgg gcaggcggtg 240
cagggcggcc agccgttggc tgatgatttc cgggt 275
<210> 574
<211> 272
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 574
ctggtgctgc tgctgttcgc actgcgcatc gggttgcgcc tgcgtctgcg cacgctgccg 60
ccaggccctc ccgcgtcgct accgatgcgt ctggccgctg gcgccacgca tgtggcgatg 120
tacggattga tgcttgcgct gccgctgctg ggctggtcgc tgagcagcgc ctacggcaag 180
caggtgtatt tcttcggcat ccccttgccc gggctggtcg cgccggacgg cgacctcggc 240
gacaccctgg gcacatggca cttgaatgcc gc 272
<210> 575
<211> 236
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 575
gtaacgcgct gtcgcaatcc acagcgcatc acgcgcgagc ctgctgcagt gcactcgcgt 60
atcgacccag gatgcgaaga gccggattga ttgcaggcga acctttggcc acacgccagc 120
ctcacgcgta acggaaaaac gcctccatcg gatctgtgac tgcagacgca atagctggca 180
ccggcagcat gccatgctcg cctgccatca cctgcacgaa tgcctgcttg aagcgc 236
<210> 576
<211> 262
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 576
aactggattc aagaatgtga gcgtaacctc gtccccccac cgcagcggta gcgttgccgt 60
gatcggccgg cccaacgtcg gcaagtccac cctgaccaat gccctggtcg gcgcgaaggt 120
cagcatcgtc tccaaccggc ctcagaccac gcgccatcgg ttgctgggca tcgcgacctt 180
cccggaaggg caattggtgc tggtcgatac cccaggcctg caccgcgagc agaagcgcgc 240
aatgaaccgg gtgatgaatc gc 262
<210> 577
<211> 234
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 577
ctgctgccgt tttgttcgta ttcgcgaacc ggcacgcgcc ctgcagcgcg ggtaggttcc 60
atttgccgcg gcgcctgggt ggttgcattg cgtcgatggg gagattgttg ctgttgccat 120
ctgtcttcgt tgcggcttgg ccgcacctgc acgggctctc cagtacgtgc aggcgccacc 180
ggtgcctgat gccgtggtgg ggggcgcagc ggattcaggt agaagggttt gccc 234
<210> 578
<211> 198
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 578
ggtttgcagc agtgcagatt gcgcctcatc acaaccggca aaacgccacg cctcaggtcc 60
ggctgccagg ctctcaacgg ctgatctgaa cgagttcagc tatttttcgc cgcggtgggg 120
tgcggccacc tgccagcagc aggatcagga gtgcgcgtac gtcgggagcg atcagggcca 180
tgcattcagg ttaggccg 198
<210> 579
<211> 253
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 579
gcgttcctgc agaactccat cggtggcgtg caaggcccgt tcgacagctt cctcgcgcta 60
cgcggattga agaccttgcc gctgcgcatg cgcgcgcact gcgaaaatgc cctggcgcat 120
ggcgcaatgg ctggaaacgc accccgccat cgaaaaggtg atctaccccg gcctggcctc 180
gcacccgcag catgtgctcg cgaaacgcca gatgtccggc ttcggcggca tcgtctccat 240
cgtcctcaag ggc 253
<210> 580
<211> 269
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 580
cgaagcggat ccgttcgtag cgctcggcga actcggggaa cgcacgccgc atcagttctg 60
agttatcggc gtcgtcccag acgtaaacag gtccagcctg gcaatagacg ctgcaagcgg 120
tcgcaaatcc tgccctcttc ccatagcaac tgcttggtgg gagcggtcag gtgaataaga 180
tgggggatca tggcgggcgg ctcggggaac tgggggaact gggggaacca gcgccgccgc 240
cgtagcgccg catcgattcg gctacccgg 269
<210> 581
<211> 268
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 581
gccagcaagg tgttgaggaa ggccagcgcc gcaccggcat tggcttccag attggaaccg 60
gcgttgaaac cgaaccagcc cacccacagc agcgaggcac cgatgaaggt cagggtcacg 120
ttgtgcggct tgatcgcctc acgtcccaac ccggcacgct tgccgacgaa gnnnnnnnca 180
accagaccgg cgacaccggc gttgatatgc accacggtac cgcctgcgaa atccagcgcg 240
cccaacccga acagataccc accggtgg 268
<210> 582
<211> 229
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 582
gtggtgcaga cacgcagaca acctgcgaca cgggcgaccg aaccgccgcg cagcaacgcc 60
tgttgtcgga aaggaagtgg gtttcgcact ggatgcgccc gtgcgctcca gtttcagcag 120
ttggtcacaa tgtgcctgtg cgtagacgct gcgtcttgtc gcatttggcg acagcgcatg 180
cggaaaacag catgttttgc aactgaacga tcatgcgtgg tggtgtgac 229
<210> 583
<211> 275
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 583
ggcaaagtcg ttcactgtat tggacggaag accgtcggcc acagtgagtt ggaccggcac 60
cggagtggtt ggtaccgccg cggaggcaaa cacgggcacc gcggtgatca atagaaccaa 120
ccaccgtgca gcaaggttgc gaccgaaagc gggacaaaaa cgtaggcgtg acataaaaaa 180
tttcactgca ggtcgccgca tagtgcaggc cgcgccgtgc atgagcaagc acgcatcgcc 240
tcactcacgt acgcccgatg cagtgccatc agcga 275
<210> 584
<211> 206
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 584
gagattggcc aggtcgacaa gcagcccacc aactgggtgg cgatcggcat gttcggcttc 60
ttcgtgctgg gcaccttgtg gatcaccaag tgggcggcgg ccaagaccaa gtcggccgcc 120
gacttctatn nnnnnncttc tataccgccg gtggcagcat caccggtttc cagaatggtc 180
tggccattgc cggtgactac atgtcg 206
<210> 585
<211> 256
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 585
gcagctggct gctgttgatc ggcttcgtgc cggtggtcgg cctgccgctg tatctgctgt 60
tcggccaccc atggttgtcg cggctgcggg tggagcggca agccaccgcc tcgcaggtga 120
ttcgcgagca gcaaactgcc actgcacgcg ctgcgctgga ccccgcaggc agacaccagc 180
agtgccgaag tggtgccgct gatcgagcgc gaaggcgatt tcatgccgac gctgggcaat 240
gcggtggagt tgctcg 256
<210> 586
<211> 275
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 586
cctcgtcctt cttgacattc atgttaagaa agacccgatt gtcattggtg atggttggcg 60
tcaccttgag ttcaagcagc acttccttag aactgcacgt tggcctgcga accgccgccc 120
gcaccactgc cgctgatggt cacatagccg atctcccggc cctgcttgat cacgccctcg 180
cgctgattcg cggtaacgat gcgcgggttc gatacgactt cgccacgtcc ctcttgctgc 240
atcgccgaca actcgatatc cagattgaaa ttgga 275
<210> 587
<211> 198
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 587
cgctggtcac gaacacaaag cgcgccgggc cgctattgcg gccgcggtgg gccccacttc 60
accagctcat agagctcgcg cagtacctcc tggctgaccg gcgtgtcggc gaacgcgttc 120
tgcgtgcggg cggtgcgaaa cagctgatcc agtgcggcgg cgttgagcga gtcggacatg 180
agcactccag aggaaggg 198
<210> 588
<211> 265
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 588
ggtgccgcag caggataaat attggtacgt gcaagatcag cgcgaccgct acggcgtcac 60
cggcaaattc gagatgcggc ctagcgcagc gctcgaagca tatgccatgg ccggttacta 120
cttatttcaa ggtaacggac atggagcgcg aacgaggtca tcatcgaccc gcgcaaccgt 180
ggtaaggtct tcaatcaaac cgctacctct ggcagctatc ccggcggcga tatcgaggtg 240
ggttactcga accagatcgt cacca 265
<210> 589
<211> 239
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 589
gcaggcagtg acatcatcag tcgagaacgc ttcacctcgt acagccgttc gcgggattat 60
ccgcgcgagt ttcctggcgt gctgggctat ggctatatcc atcgcgtcgc cccggccgaa 120
cgaagacgcg ttttttgcag aatgcgcgtg ccgacggtgc gccggacatc cagcgccggc 180
cattggcgcc atgggatggc gagcgctaca tagtgttgta tttcgagccg gaatcctcg 239
<210> 590
<211> 272
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 590
gtgccgtatg gcgacaatct gcgcgccgcc tatgcggcga ccaaggccat tgccgagcgt 60
gcagtgctgg ccgccaacga cgcccagctg gcgacagtgg cactgcgccc gcgcctgatc 120
tgggggccgg ggcgacaatc acttgttgcc gcgcctggca gcgcgtgcgc gtgccgggcg 180
gctgcgcatg gtcggcgatg gcagcaatct ggtcgacagc acatacatcg acaacgcggc 240
gcaggcgcat ttcgatgcgt tcgagcacct tg 272
<210> 591
<211> 275
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 591
gccaacgcat cgtcgatcac gacggcaaca gcgccaccga tggcggcatc gcgcgctggc 60
tgcatctgac cgacgcgctg ggcctggacc gcacgctggt ggaatccggg cgcgcgctgc 120
tgcccggtac ccgctttgcg gtgcaggcgt atctgcagtt cgtaagcgaa aaaaagcctg 180
ctcgaagcga tcgcctcgtc gctgaccgaa ctcttcgcgc ccaacatcat cggccagcgc 240
gtggccggca tgctcaagca ttacgacttc gtgtc 275
<210> 592
<211> 246
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 592
cgcgttcttc cagagtgtgt acggacgcaa ttcgatcgat gccaagcgca tgccgctgat 60
cggcagcgtg cattacgagc gtggcgacga caacgcattc tggaacggcg agcaaatggt 120
gttcggcgat ggcgacggcg aagttttcaa ccgcttcacc atcgccatcg acgtggtcgg 180
ccatgagctc acccacggcg tcactgagcg caccaccaac ctgatctatc aaaggcaatc 240
cggtgc 246
<210> 593
<211> 227
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 593
gcgccttttc gatcgcttgc agatcgaaac cgtggtgcat cgggaattgc tgatcctccg 60
cagctaccac cgaaatcggc aggctcgcgg cgatcttgtc gtaatcacgc catcgctgat 120
gcagcgaaaa actccagtcg ccctcgcccc aggcttccac gataacgccc caccatccat 180
gctgctgatc ggtggatcga ccacccgcaa gaacagcacc tgcagca 227
<210> 594
<211> 273
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 594
cgtcaaggac gatctgattg ccgtgcccga cagcaccttg cactggttcg acatggggcc 60
ggaaccgcac ttcgtggcga ttcgcttctt caccgagccg gacggctggg ttggacattt 120
caccggcacc gaaatccgcc aaaacagttc ccccgctacg cccccgagaa accctcacaa 180
ggccagctga cgatgacacg accgcaagcg atcctcaccg atatcgaagg tacgaccagc 240
agcatttcgt tcgtcaagga cgtgttgttc ccg 273
<210> 595
<211> 264
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 595
cgtattggga cgcggaaatc aacgcgcgac aatcgaaggg catcccggct acccggtgtt 60
taccccggca agcaaaataa ccgacgtgtc gtatctggcc tgcgcacgtc gcatgttcgc 120
gcacaatgac gcgctctatc cgatgttcgc cacgcataac gcgcagacca ttgcggcggt 180
gcgtgcgatt gcagcaggca agacttacga gcatcagaaa ctgcacggca tgggcgatga 240
cctgtatgcc gaagtgattc cagc 264
<210> 596
<211> 211
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 596
atgcatttca agagctcagt caaccgtgcg ccgcgccaat ccaccaccgg cgaagagggt 60
gaggagcggc tgctgaagct ggaactcagg ctgcttgcgg atgtgggtct gctgggcttc 120
ccgaacgccg gtaagagcac gctgatccgc gcggtgtcct ccgcaacgcc gaaggtggcc 180
gactacccgt tcaccacgct gtatcccaat c 211
<210> 597
<211> 258
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 597
gcggttgttg atcatcgcta gcgtgttacg caaattgtcc tgacgccgct ggcccctggc 60
caggttggcg aagttcgtcg tgttgcgcgt cgtggccgag gggcggtaat cctcatcgaa 120
gcgcaggctc ttgatggtga agttcaagta ttgagtcatt gcgagctgat gcctggtgtc 180
taggatggga atgcgcgtct gtcgctgcgc aggtggtaat gccggcggca tgcagaccgg 240
gcagttggat cggtgccc 258
<210> 598
<211> 255
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 598
cacgtccaga tccaggtctt cgtgcacggt ctggcgaccg aaccggttgg tcagcccgcg 60
cacggaaatc gccaactgat cgtcgtgggc gtcagtctcc gggccgggat gggccagtgg 120
ggattcggga gtgatcacag cacttctctc ccaaataccg cgacaagcgt cgcgctgcgg 180
ttttgcacga atcccagatc ccgaatgtcg aatcccgcct ccatcaccac cccatcttca 240
tgaaccacaa cgccg 255
<210> 599
<211> 238
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 599
cgaggagcgt tgccgatatg gcctgtcgcg cctggatacg ctcctgcggg tgaggtcgat 60
gcctggccgc gtcggtgaga cgccgatcag agcgcaggtg cctgagcgcg cggtcaagac 120
tgccgcaagt ccagcgccgc catcacgatg ggcttagccc aatcgggcac ctgcgccaat 180
tcttccggcg tttgcgggta atgcagaccg ctgcggtcca gttccagtac cagcacgg 238
<210> 600
<211> 209
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 600
cgctggtcgt agatggtttt gacgcgtgcg aagagttggg aatcgagatt gatctcgtcc 60
tgatgcgcag ccagcttggg cgcaatctcc tcctggattt tctgacgtgc gtcgctggta 120
tcggcctgca ccagcccgaa gaagatgcgc gagacgcgcg tgagcgtttc gccaccgcgc 180
tccatcgctt cgatggtgtt gtcgaaggt 209
<210> 601
<211> 275
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 601
ggaggcgatc ttccagaaac ctgccttcca gttctccacg cgtttgcgcg tgccttcggg 60
cgtgatcacg taccacatct gctcggagtt gcggatcagg cgcaccgcct ggccgatagt 120
gccctgcggc aagctgcggt ccagcgggat ggcgccgagc ttgcgcagca gcgggcccag 180
cggccaccag aacaactgcg atttgccgag cacacgcacc tcgaagccaa gcgcgaactt 240
ggccgcgaac cccacaaagc cgtcccaatt ggacg 275
<210> 602
<211> 211
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 602
gcgtgctggc agaaaacttc aaggttggcg ccgagctcgg cttcaagttg cacaacgcca 60
atgtcgaggt gctgcgtgcc gagttgaacg acaagcacgg gcgtagcgcg cagctgcagc 120
agtggggggt gctcagcgac ggcacgcgca ccgggccgcg gctactggtg ctctcgccca 180
gcgatcagcg gtatcgcgag ctgttgaagc g 211
<210> 603
<211> 232
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 603
gggaatgtag gtaatggcgt gattgaagcg gcccagcacc ggaatgtccg gcagcgtcgg 60
gccgccctcg gcgccgatca gcactggcgt gctggcgatg cccttggccg caagcaaagc 120
ttccaggatc acggtgtggt ctttgcaatc accgtaatgg ttggccagga tgctgtcggc 180
acttggttgg gctccacgcc cgccattgcc cagatacacc gcgacatagc gg 232
<210> 604
<211> 187
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 604
cggcgaccaa taacgacatt ctgaactcgc gaaacattga cactcccagg cgggccgaga 60
cgacagccgg aacaacgggc cagggcgagg cccggcagat ccggcgtgcg atggaggatg 120
ggggaccgcg gcgtgacacg acgtcaggcc gggcgcggct cgcccattat agggagtgtc 180
accgcca 187
<210> 605
<211> 182
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 605
catttcgttg gcaagcaact caccgtcgcg cacgaacagt tccagcgcaa acacgccaac 60
atagtcgagc ctttccgaca cggcacgcgc gtgcgattcg gccgatgcct gcagcgccgc 120
atccacgcgc gccggcgcga gactggccga caacacgcca tctacgtgcc agttctcagt 180
ca 182
<210> 606
<211> 275
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 606
catctgctgc tggacctgtt caaccaggcg cgcgaaaagg ccgaggccga ggcgatcgcg 60
gtgttcggcg acaacctcaa agatctgatg ctggcggcgc cggccggccc gcgcgtgggt 120
tctggggctg gacccggggc atccgtaccg gttgcaagat cgccgtagtc gatgccacag 180
gcaagctcgt ggccaccgaa acgatttatc cgcacgagcc caagcggcag tgggatcagt 240
cgctgcagac catcaaaaag ttgtgcatgc agcac 275
<210> 607
<211> 201
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 607
gctgaaggtg gtgccgtatt acccgatcag tgacgacgtg ctggcgcaga tcatcgcgct 60
caagctcggc cgcatccgcg accgcgtggc caccaaccac aaggccgcct tccactggga 120
ccagagcctg gtggaggcgg tgttggcgcg ctgtacggaa gtggattcgg gtgcgcgcaa 180
cgtcgaccac attctgaatg g 201
<210> 608
<211> 264
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 608
gcagtcgcgt tcgacaactt gcgagagcgg cgcaatcagg cggcggactg gcttgaacat 60
gcgcacagga taccgctgca atgtgtcagg cggtgagacg ctgcgattga tgcaattcga 120
tgctggccac tatttgagcg ggcggcaaaa caaccagggc ctccgctagt tgggcgcggt 180
cgacatcggt agatctgcgg cccggctgca gggccagtgc ccgccaccat cgcaggacac 240
ggcgcaagta cgtccgtttc ggcc 264
<210> 609
<211> 239
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 609
gcggatgctt cttcttcgat ctcatggatg cccgcaggca atgccggcgc ggtgcgattg 60
agcggagtgg cggatgcagc aggtttcatc acatttctcc tggtgcttga tgcagtggcg 120
gcatcggtca atggccgctg ccgggtaatg caatcgtcaa ccagcctgga actgggcgta 180
ttgtccgtgg gggcgtggtg agccagtggc ggcagaggag atcgatcagg gcaaattgc 239
<210> 610
<211> 182
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 610
cgtgcgagat cgtgttgcat accgactcgc aatacgtgcg ccagggcatc accgagtgga 60
tgccgggctg ggtgcggcgc aaactggaaa accgccggcg gcgatccggt caagaatcgc 120
gagttgtggg agcggctgca cgcggccacg caacgccacc gcatcgattg gcgctgggtg 180
aa 182
<210> 611
<211> 253
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 611
gatgggagaa ggaatccacg ttaccgcgct gcaacctcgc ccactgccgc actacccaca 60
tacgtccgcg gcacgcgctt gagtccacac aacagctgat acgcgctgac attgcattgc 120
gtggccagtg ggccaacgcg tggtgcctgg ccccacagct gcacggtggc accaatggcg 180
gcctgcgggt ggtcggtaag gtccacggtg agcatgtcca tcgagacgcg accgatcaat 240
gggcagacct ggc 253
<210> 612
<211> 275
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 612
tttttggcct ggatcgactt caccaccgcc tcgtagcgcg tgttggcctc gaccaactcg 60
gtgttggtgt acttctgcca ccactgccca cccccacgga tcaggaccca gcccaaccca 120
ggacgccgcc caccagaccc gcgccattgt ttctcaacca ggaacgaacg cgttcgcctt 180
gttcgtgttc gtccagcaga tcgtcgatcg ccatgcgtac tctcgctccg tcatcccgaa 240
ggcgcggaac atcaataggg ataaatgaaa aaccg 275
<210> 613
<211> 177
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 613
tgttgaggaa caggttggca tacggcgtgg cgccgaccac cggggtgcca tccggggtgg 60
cagggcgcag gccggtccag aattcggcct gggccagatc gccactgccg gggaacaggt 120
cgttgaccac catttccagg gtggcgcggc gacgcagatt cagcgacatg tcgaagc 177
<210> 614
<211> 229
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 614
gtcgcgttgc tccttgtcgc ggcgcaaggt ggccagcgtg gccagttggt gctcgcgcag 60
cacatcgggt ttggggggcg gcgtcagggt cgcgctcggc cttgaccacc gccacctgcg 120
cggtgccccc atcggcgcgc cagtggtacc agttgttgcc cgcgcgccag atcgcaccgc 180
cgtcgctggc gaactgcgga cgcgattcga tctcgttgct gcgggtcag 229
<210> 615
<211> 191
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 615
gctgaccaag gcgcaattga atatcccggc acctccggca gtcacctcga tcaagccgag 60
ccagccggcg ccggtgcgtg aagagacccc cggccccggt ggcgcccgtc gccgctccgg 120
caccggtggt gactgtgccg attccggccc cggtcaccgg cgtggtcggc tggttgaagc 180
gcatcttcgg c 191
<210> 616
<211> 201
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 616
acggtgcgtt gtttcgaaga taactcgcgg atgcgcgagc tggccgccac gccgggcgat 60
gcccgggtgc tggtggtgga cggccagggc tcgctgaagc atgcgctgct gggcgaccag 120
atcgcgacca acgcggtggc gaacggctgg gccggcgtgc tgatccatgg cggcgtgcgc 180
gatgtcgaga tgcttgcctc g 201
<210> 617
<211> 206
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 617
gcggctaaag aacgcaattt ctttaggggt aattttcttc cccgcccctc cgaacagagg 60
cttcggcttt tgcttcacca tgctgggcaa gatgccctga cggcgcagct ctgctcgcaa 120
catattggcg ttgcgggctg tctgctctcc cttcatcttc acgccacgct tgtcagcccc 180
ttcccaaatg aaggtctgca gcatgc 206
<210> 618
<211> 275
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 618
cgcttacacg ctgatggtca agcagaccgc cgacgcgctg cacaaggccg gtttcaagat 60
gtcggtcgcc gtggtgccga atgcgccggg ctatgccgaa ggcggccagt tctccaagtg 120
gatgtgggaa tactggcgtg gtgcgtacga cctgaaggcg ctgggccagg cggtggatct 180
ggtcagcctg atgacctacg accagcacac ccgctggacc acgcccggcc cggtcgatgg 240
catggtgtgg atgaagaagc atctggatta cgcca 275
<210> 619
<211> 241
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 619
ggtgttcgga atcggcatgt gccacacgta cattccggct ctaggcacgc cgtccacgcc 60
ccgctgaaaa ctgcgcgcga cgtttaggtc gccgctccaa gtgagatgac gcaccaccgc 120
acgcagtcac gggcgcggct agaactggaa tcgagctgca tgtactgcac cgcggctcct 180
gcgctatcgg cgcccgtttt gcagcgaccg atgacgttgt attggtcgct ctacaacgca 240
g 241
<210> 620
<211> 229
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 620
cggcaatatg tgcgacacaa cccgcccggc gttggcgcgg cggtatcacc gcgaattcgg 60
cgtcccgatg ctgttgtacg tggtggtgat gctgttctgg cgttatctgc ttgcgtatgt 120
cggccctgac tgggcgcgtg tgctgatcgc cttgctgccg gcggtgctgg tcgtgctggt 180
gatccgtgcg gtggcgcgct atgtgcgcga ttcggacgag atgcaacgc 229
<210> 621
<211> 176
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 621
gcgatgttgt tcgtcgaaca attccgcgta ccgctgcagg cgcgcaccat cgaaatgccg 60
gctgggctgg tgggcggatg tgcacggccg aatgaatcga tcgaactgtc ggccattcgt 120
gaactggaag aagaaaccgg ctggactgcc gagcatgccg aagtactgat gatcgg 176
<210> 622
<211> 249
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 622
gcgctgatcg aataacacga tgcggtactt ggccgggtcg tggaagcgtc gcatcttgtc 60
gttgcagccc gccggccccg ggccgccatg cagcatcacc accggcttgc cctgcggatt 120
gccacattgc tcgaaataca gcgtgtggcg gtcgtcgacc ttcagcatgc tgctgtcgaa 180
cggttcgatc ttgggatata gcgcgcgcat ggtggctccg actcaaagac ttggatttta 240
cccaggtta 249
<210> 623
<211> 255
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 623
gtacagacca gtggcgcggg cgattcccag cccatcgcgc taccgccgca attgcccggc 60
aaccgtgcgc gcaatcggcg cgtcgagatc cctgttgact tcccgacgac ctgacccatg 120
cgctttgtgc tctgatttcc tgcgcgtccg ctacctcgcc agcgctgcca tcctcagtct 180
gttggccctc ctggtgtggg tcggcggacc ctatctcagc ctgggccgct ggcagccgtt 240
tgccagcgtg agcgg 255
<210> 624
<211> 235
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 624
gctcacgctg gacttctaca agcgcaatta catcgaaggt ctgttcctgt ccaggcggca 60
tcatccgcaa ctccgactac accatggagc agctgggtgg aagtagcgcg gcagttgcgc 120
gaggtgcacc gtttcgccgg ctatatccac ctcaagacca tcccggatgc aacgccggaa 180
ttacctggcc gctgccgggc gttacgccga ccgtttgagc atcaacgtgg agttg 235
<210> 625
<211> 265
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 625
gggtgggctt gtcgaaatag atcatcacat tgcgacagaa cagcgcggca tacgggccgc 60
cgacgtcgta acgcgacgcc aatagattca actggcggta ttcgatgagt tgacgcagcg 120
ccggaatgac tcggcacttg ccttcgttgg gaccgctgcc gcgctgaaaa atattttgcg 180
cttgatcgag gcgtccagcg aggcgacacg gtcgatggca tagacgccgc gcgacgcggt 240
ttccaacact tgggtatcga cgtcg 265
<210> 626
<211> 260
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 626
cgacgtcgtc gcgcatatgc gtgtacgccg gcgccagcat caccgggcca tacggcgtgt 60
gcagctgctc gcgcaccgcc tctagcagcg ccgcacgttg ctccgcatcg gcagtaccgg 120
ccagcagcgc gaaactctgc ggattgagat agatgcgccc ctcccacatc ctccgcgatg 180
cgaaacgcac gccgtcgtcg gtgatgccac gtgcgtacca gttgccatcc cacaactcac 240
gattggcatc ggcgttgatg 260
<210> 627
<211> 217
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 627
ggccagccca tagatgacaa agccgatcac cccgccagcc cagccccagc agcaaggcgc 60
ggggttcgcc gagccagcgc accaggcggc cgaccagcaa cacgttgacg atgatgctgc 120
acacgccgac gccggccagt acccaactca cttcgcgcgg gccccagtga tattggtagc 180
cggcgaacag cacgaagatg ctgggataca cgtagtg 217
<210> 628
<211> 211
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 628
atccgattcc accagcgaca acctcgacac ccggctgcgc gattcctcgc cgcatacctt 60
gcgcgaaatg ggcgccggct ggtgcagcaa ctcacgcccc ggctcggtgc tgttccgcca 120
cggcctgcgc atggccatcg cgctgctggt cggctacgcc atcatgcaat cgatccatgc 180
cgacaacggg tactggatcc tgctcaccac t 211
<210> 629
<211> 260
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 629
atcgagcaga gcgagctgaa cctggaagag cacgatgccg ccggcggtaa cgaggtcggc 60
aaacgcccga accaggagtt cgacaagatc gtgtcgttgt tcgtggccgc caaggcgggc 120
caggtgctgc gcggcgaaga tatggtggtt gctgccgaaa agaccggact ggtgttcggc 180
cacatgaatg tgttccaccg cctgatggaa gggcacccgg agcgcgggcc gattttcagc 240
atggcaagca tcctcaagcc 260
<210> 630
<211> 199
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 630
cacgttgttg agcactgcat atcgcgtcat cgaaaactcc agcagccgtc cgtcgcggag 60
gactgcagtc ataaagggat tcggtcgcac gcagtccccg ccgtgatggc gatgctgagc 120
attgccaaaa atgtgacgcg ccggcaagcc gcagggcagc aatgcgctgc gtcactgtga 180
aggtaagcgc gtacgtcat 199
<210> 631
<211> 220
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 631
cggcgtagtc gaattgcttc tttgctgccg cttcgttggt ctcggctgcc gccagcgact 60
cgatccccac ccagtacctg gaaatacagc cgccgaggta cgcgtcatca gatcgttgtt 120
ggccgatgcc aaagtgaaat ctgcagccct ggtcacttcg cgctgcgcgc gcaggttaga 180
gaactgcgcc caattgaaaa tggtctggct accctgaatc 220
<210> 632
<211> 185
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 632
ctgggcctcg atcacttcat gcgccaggcg attgatcacc ttcgggtcga tgccgtaatc 60
cccgtctccc catcagcgcc tccccggaaa gtttgagaag aatgcggcga taggaaagtt 120
cggacatggg ggaggcctcg gggtagggaa tctgcaaacc ccggcaattc tagcggaagc 180
ccgtc 185
<210> 633
<211> 215
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 633
gcgagctgga atacgtggat cgcggcaccc tgtcgcacgc cttcggcacc cgcagtgagg 60
tgctgtggag ggcggggatt ggggaggcgg gatttgggat tcgcaaagcc gtgctggcgc 120
gcacggtaag ctgttaccaa tcccgaatca cgaatttcca atcccgaacc atgaccgaca 180
ccatcttcgg caaaatcatt cgtcgcgaaa tcccg 215
<210> 634
<211> 253
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 634
ccgtgtatcc gatcaaggtc aaccaacacc gcggcgtggc cggcaccctg gccagccatc 60
atggcgacgg tttcggcctg gaagcgggca gcaagccgga gctgatggcg gtgctggcgc 120
tgtcgcgtcc gggcgggctg gatcgtctgc aacggctaca aggaccgtga atacatccgc 180
ctggcgctga tcgggcgcaa gctcggcctg cagaccttca tcgtcatcga aaagccttcc 240
gaactgaact tgg 253
<210> 635
<211> 259
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 635
aacctgcggc tgaggcatcg acccaggcgg ctgcagctgc ggtgtcttcg ccagcgcagg 60
cgggtgcatc ggcggcgaaa agcgaaccag cttcatcgcc gaaaccgaca ccgacaccga 120
ccaccgacgc agcaaggcca gctgccgagc tggtcgagcg ccccgacacg gaccaagcac 180
cggaaaatgc gccggaaccg gtgcaagctg ccagcgaacc cgtcaccgca gatgttgcgc 240
aagtgacggt acaggtgcc 259
<210> 636
<211> 275
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 636
tttaggcgta ggcagtgctt cgaccaggcc gtcgcggcgc gcgctgcaat cgtgctgcag 60
cgggcacagc acgcacgcgg gtttggcgct gcgtgcacag cgtggcgcca aaatccatct 120
gcgcttgtgt gtagtcggcc agccggccgg gcgggacgtg tgcgacatgc gcagcggcca 180
gttgccacaa ctgtttttcg atcaccggca ggccgggata gccgccgata ccgtgaaagc 240
gcgtgagcac gcgtttgacg ttgccatcca tgatg 275
Claims (8)
1. The MNP marker locus of the Xanthomonas oryzae is a genome region which is screened on a Xanthomonas oryzae genome and has a plurality of nucleotide polymorphisms in a Xanthomonas oryzae population, and the MNP marker locus comprises marker loci of MNP-1-MNP-212 on the rice genome; the marker site sequences of the MNP-1-MNP-212 are shown as SEQ ID NO. 425-SEQ ID NO. 636.
2. A PCR primer composition for detecting the MNP marker locus according to claim 1, wherein the PCR primer composition comprises 212 pairs of primers, and the nucleotide sequences of the 212 pairs of primers are shown in SEQ ID No.1 to SEQ ID No. 424.
3. A test kit for detecting the MNP marker site according to claim 1, characterized in that the kit comprises the primer composition according to claim 2.
4. The detection kit according to claim 3, wherein the kit further comprises a PCR premix.
5. Use of a MNP marker site according to claim 1 or a primer composition according to claim 2 or a test kit according to any of claims 3-4 for the detection of xanthomonas oryzae in cultured and non-cultured samples.
6. Use of the MNP marker site of claim 1 or the primer composition of claim 2 or the detection kit of any one of claims 3 to 4 for the detection and identification of genetic variation in xanthomonas oryzae.
7. Use of a MNP marker locus according to claim 1 or a primer composition according to claim 2 or a test kit according to any of claims 3 to 4 for the detection of homozygosity and heterozygosity in a xanthomonas oryzae strain.
8. Use of the MNP marker site according to claim 1 or the primer composition according to claim 2 or the detection kit according to any one of claims 3 to 4 for constructing DNA fingerprint database of xanthomonas oryzae.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116904620A (en) * | 2023-03-23 | 2023-10-20 | 三亚中国检科院生物安全中心 | Primer group, kit and method for rapidly detecting rice pathogenic bacteria based on MNP |
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| KR20120053792A (en) * | 2010-11-18 | 2012-05-29 | 대한민국(농촌진흥청장) | Primer set of polymerase chain reaction for detecting the genus xanthomonas and method for detecting the genus xanthomonas using the same |
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| CN116904620B (en) * | 2023-03-23 | 2024-04-30 | 三亚中国检科院生物安全中心 | Primer group, kit and method for rapidly detecting rice pathogenic bacteria based on MNP |
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