CN114774409A - Secondary sequencing detection system based on 224 InDel and 57 SNP sites - Google Patents
Secondary sequencing detection system based on 224 InDel and 57 SNP sites Download PDFInfo
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Abstract
The invention provides a second-generation sequencing detection system based on 224 InDel and 57 SNP sites, which comprises a primer combination, a kit and a matched DNA typing method. The primer combination can simultaneously amplify 224 InDel loci and 57 SNP loci, wherein the InDel loci comprise 178 autosome InDel loci, 38X chromosome InDel loci and 8Y chromosome InDel loci, and the SNP loci are phenotype information autosome SNP loci. The detection system of the invention applies the single-tube composite amplification and second-generation sequencing technology, can obtain the genetic marker information of candidate InDel and SNP sites on autosomal chromosomes, X chromosomes and Y chromosomes of a plurality of samples at one time, reduces the operation steps, shortens the experimental time, and can be used for the application research of forensic individual identification, assistance of complex genetic relationship identification, ancestral inference and phenotype inference.
Description
Technical Field
The invention relates to the technical field of gene detection, in particular to a second-generation sequencing detection system based on 224 InDel and 57 SNP loci.
Background
Insertion/Deletion Polymorphism (InDel) refers to a genetic marker of length Polymorphism formed by Insertion or Deletion of DNA fragments of different sizes in a genome. Single Nucleotide Polymorphism (SNP) refers to a DNA sequence Polymorphism caused by variation of a Single Nucleotide at the genome level. The two genetic markers are widely distributed in the human genome and mainly show genetic variation of two alleles, and the characteristic makes the typing result easy to analyze. The amplification product of a single InDel or SNP site can be controlled below 150bp, which is beneficial to the detection and typing of degraded test materials. These two genetic markers are relatively stable, and their mutation rate is significantly lower than Short Tandem Repeat (STR), but the genetic polymorphism is also lower than STR genetic markers. Therefore, in the actual application of forensics, more InDel and SNP loci are subjected to typing analysis simultaneously, and the forensics efficacy obtained by the conventional STR genetic marker combination can be achieved.
According to different practical effects, the InDel and SNP loci commonly used in forensic medicine can be divided into individual identification genetic markers, ancestral information genetic markers, phenotype information genetic markers and pedigree information genetic markers. The individual identification genetic marker has the same function as the selective STR locus and provides genetic information for individual identification. However, InDel and SNP loci have higher genetic stability compared with STR loci, so that the mutation probability is lower, and better individual recognition capability can be achieved. The ancestral information genetic marker refers to polymorphic sites with large gene frequency difference among different populations, and is mainly used for deducing individual biological geographic sources and ethnic sources. A group of ancestral information genetic markers can analyze the genetic component composition of a certain population and also can deduce the ancestral source of a certain individual. Phenotypic informative genetic markers may be used to infer specific phenotypic characteristics of an individual, such as skin color, hair color, or iris color, among others. Pedigree informative genetic markers are typically linked loci on the Y chromosome or mitochondrial genome that can be used to identify or exclude unknown individuals by family relativity analysis. Therefore, more genetic information can be obtained by typing multiple types of genetic markers simultaneously.
At present, Capillary Electrophoresis (CE) technology is mainly adopted for typing detection of InDel and SNP sites in the field of forensic medicine. However, the CE technology is used for typing detection of SNP sites, multi-step amplification and digestion are needed, and the experimental steps are complicated. Moreover, the CE technology can only detect tens of sites of a small number of samples at a time, and the obtained genetic information is extremely limited.
Next Generation Sequencing (NGS) is also called Massively Parallel Sequencing (MPS). Compared with the first generation sequencing technology, the method is not influenced by the type and the quantity of fluorescent substances. The high-throughput characteristic of the kit can realize simultaneous detection and synchronous analysis of STR, SNP, InDel and other multiple length polymorphisms and sequence polymorphism genetic markers of a large number of samples. Therefore, the NGS technology has certain advantages for the simultaneous detection and analysis of InDel and SNP sites for individual identification, ancestral inference and phenotype inference.
Disclosure of Invention
The invention aims to provide a next-generation sequencing detection system based on 224 InDel and 57 SNP sites, so as to solve one or more technical problems in the prior art and provide at least one beneficial selection or creation condition.
In a first aspect of the invention, a set of primer combinations is provided. The primer combination can simultaneously amplify 224 InDel sites and 57 SNP sites, wherein the InDel sites comprise 178 autosomal InDel sites, 38X chromosome InDel sites and 8Y chromosome InDel sites, and the SNP sites are phenotype information autosomal SNP sites.
Further, the nucleotide sequences of the primer fragments in the primer combinations and the information on the amplification sites corresponding thereto are shown in table 1.
TABLE 1
The PCR amplification usually adopts a pair of upstream and downstream primers to amplify a target fragment, and primer fragments of nucleotide sequences shown in SEQ ID No.1 to SEQ ID No.562 of the application are pairwise paired, namely the sequences shown in SEQ ID No.1 and SEQ ID No.2 are a pair of primers, the sequences shown in SEQ ID No.3 and SEQ ID No.4 are a pair of primers, and so on until the sequences shown in SEQ ID No.561 and SEQ ID No.562 are a pair of primers. Thus, the primer set of the present application actually includes 281 primer pairs covering the 224 InDel sites and 57 SNP sites defined herein.
The 224 InDel sites and 57 SNP sites which are specifically amplified by the primer combination cover the whole genome of a human except a plasmid, and the genetic marker information of candidate InDel and SNP sites on an autosome, an X chromosome and a Y chromosome can be simultaneously obtained only by one-time amplification, so that the operation steps are integrally reduced, the experimental time is shortened, and the primer combination can be used for the application research of forensic individual identification, assistance of complex genetic relationship identification, ancestral inference and phenotype inference.
A second aspect of the invention provides a kit. The kit comprises the primer combination.
Further, the kit may optionally include the following reagents: PCR buffer solution, DNA polymerase, DNA standard, DNA extraction reagent, PCR amplification reagent, sequencing library construction reagent, nucleic acid purification reagent and deionized water.
Further, the DNA standard comprises 9948 genomic DNA and/or 9947A genomic DNA.
The kit provided by the application comprises the DNA standard substance, can detect the typing result of the DNA standard substance while detecting a sample, and can effectively ensure the typing accuracy.
The length of the composite amplification product of the kit is only 125bp at the shortest and not more than 224bp at the longest, and the kit can also be used for analyzing and detecting common degradation detection materials in forensic medicine, and can realize the application in individual identification, genetic relationship identification, ancestral inference and phenotype inference.
A third aspect of the present invention is to provide a DNA typing method. By using the kit and matching with MGI-2000 sequencer (Chinesemedicine), the second generation DNA sequencing technology can be used for sequencing the sample to be tested. The MGI-2000 sequencer adopts microarray DNA nanosphere sequencing technology (DNBseq technology), and can realize high-precision gene sequencing by integrating an ultrahigh-density microarray chip and PCR-free DNA nanosphere technology (DNB, DNA Nanoballs). The MGISEQ-2000 sequencer is used as a full-function desktop sequencer, a brand-new slide glass system is adopted, various different sequencing modes are flexibly supported, and an optical and biochemical system with optimized design is adopted, so that a complete sequencing process can be completed in a short time. The single operation of the system can complete the off-line data of 600G at maximum, and the full-load operation in the PE100 read-long mode only needs less than 48 hours.
Drawings
FIG. 1 is a schematic diagram of the localization of 224 InDel sites and 57 SNP sites in the human genome;
FIG. 2 is a graph of individual ancestral component analysis results based on 120 ancestral source information InDel;
FIG. 3 is a graph of principal component analysis results based on 120 pieces of ancestral information InDel;
fig. 4 is a graph of the results of phylogenetic analysis based on 120 pieces of ancestral information InDel.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to specific embodiments, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.
The embodiment of the invention provides a detection system based on a second-generation sequencing technology, which is constructed by utilizing a second-generation sequencing platform which is rapidly developed at present based on 224 InDel sites and 57 SNP sites. The detection system comprises primer sequences designed aiming at 224 InDel sites and 57 SNP sites, and each pair of primer sequences corresponds to different sites, so that the detection system can simultaneously detect the 224 InDel sites and the 57 SNP sites. The detection system is finally composed of a composite amplification primer mixture, a DNA standard substance and a composite amplification reaction mixed solution.
The 224 InDel loci include 178 autosomal InDel loci, 38X chromosome InDel loci, and 8Y chromosome InDel loci; 57 SNP loci are phenotypic information autosomal SNP loci. The positioning of 224 InDel sites and 57 SNP sites in the human genome is shown in figure 1.
Specifically, 178 autosomal InDel sites and corresponding primer sequences are shown in table 1. 178 autosomal InDel sites are distributed on 22 pairs of autosomes respectively, and corresponding primer sequences are shown as SEQ ID NO.1 to SEQ ID NO. 356.
The primer sequences corresponding to the 38X chromosome InDel loci are shown in SEQ ID NO.357 to SEQ ID NO.432 in Table 1.
The primer sequences corresponding to the InDel sites of the 8Y chromosomes are shown in SEQ ID NO.433 to SEQ ID NO.448 in Table 1.
The primer sequences corresponding to the 57 SNP loci are shown in SEQ ID NO. 449-SEQ ID NO.562 of Table 1.
Example 1
The method adopts the detection system based on the second generation sequencing to detect the DNA by typing, and simultaneously amplifies 224 InDel sites and 57 SNP sites in the sample DNA in the same PCR amplification reaction system.
The PCR amplification reaction System for target region amplification is shown in the following table 2, and comprises 10. mu.l of PCR buffer solution containing DNA polymerase, 8. mu.l of primer mixture, 8. mu.l of 2 ng/. mu.l DNA template and 4. mu.l of deionized water, wherein the primer sequence in the primer mixture can simultaneously amplify 224 InDel sites and 57 SNP sites in the template DNA under the action of the DNA polymerase, and the PCR amplification System can obtain better results on various reaction thermal cyclers (such as ABI9700, ABI ProFlex 96Well PCR System, ABI Veriti and the like) by adopting the PCR amplification conditions shown in the table 3. Wherein, the PCR cycle comprises four cycles, the first cycle is 1 cycle under the condition that the temperature is 98 ℃ and the time is 3 min; the second cycle comprises 20 cycles of two stages, the two stages comprising a first stage at a temperature of 98 ℃ for 20s and a second stage at a temperature of 62 ℃ for 4 min; the third cycle is 1 cycle at 72 ℃ for 2 min; the fourth cycle was 1 cycle at a temperature of 10 ℃ for an unlimited time. DNA can be prepared from peripheral venous blood, blood stains, buccal swabs, hair with hair follicles, and the like.
The template DNA is amplified under the PCR amplification system according to a specified reaction program, and mixed amplification products of all the sites can be obtained.
After the first round of PCR reaction is completed, the PCR product is subjected to purification treatment.
Then, a second round of linker addition reaction was performed using the 281 purified DNA amplification product fragments as templates. The adapter reaction system is shown in Table 4 below, and the adapter reaction conditions are shown in Table 5 below. And (3) respectively adding a joint to each sample (when a plurality of samples are sequenced simultaneously, different samples are respectively added with different labels on the basis of joint addition to distinguish the samples).
And performing secondary purification on the PCR amplification product after the joint and the label are added, and recovering a final product.
And performing library mixing according to the required data amount of each sample. And performing machine-on operation such as machine cleaning, library cyclization, Make DNB and the like according to an SE400 sequencing reagent specification matched with the MGI-2000 sequencer, setting a Barcode file, starting sequencing and analyzing sequencing data.
TABLE 2
TABLE 3
TABLE 4
TABLE 5
The invention provides a kit, which is a PCR amplification reaction system and comprises a primer mixture, a PCR buffer solution containing DNA polymerase, a DNA standard substance and deionized water, wherein the ratio of each component is shown in Table 2.
Wherein, the primer mixture comprises primer sequences designed aiming at 224 InDel sites and 57 SNP sites in a second-generation sequencing detection system.
Under the condition that the number of detection sites of each sample is certain, in order to realize the detection of more samples at a time and achieve high site coverage, the balance of the amount of DNA of each sequencing template needs to be noticed, and the difference of amplification efficiency of different primer pairs needs to be noticed, both of which will affect the library construction result, so special attention needs to be paid when primer design and primer pool mixing are carried out.
The kit of the invention introduces a DNA standard substance positive control as quality control to evaluate whether a single sequencing result is reliable. The positive controls provided in the present invention include standard typing results for all 224 InDel sites and 57 SNP sites of male DNA standard 9948. When an unknown sample is detected, the DNA standard substance is amplified and sequenced, and the reliability of the sequencing result is evaluated by analyzing the typing result of the DNA standard substance, so that whether the subsequent analysis is carried out or not is determined.
In addition, we extracted the genotyping data of 120 ancestral information InDel of 26 global groups in the thousand human genome project, and performed Admixure, principal component analysis and phylogenetic analysis. The results of individual ancestral component analysis based on 120 ancestral information InDel (fig. 2) show that 26 global populations can be divided into four major clusters according to intercontinental distribution: african, east asian, european and south asian clusters, with the american dispersed among these clusters. In fig. 2, the optimal K is 5, and the proportion of each population is proportional to the sample size of the population. The results of the principle component analysis (FIG. 3) and phylogenetic analysis (FIG. 4) also revealed several intercontinental clusters described above. In summary, the progenitor information InDel contained in our constructed next generation sequencing InDel assay system can differentiate intercontinental populations.
Based on the screening of the sites and the design of the primers, the application of the kit in the aspect of forensic DNA typing detection comprises the following steps: (1) personal identification; (2) auxiliary genetic relationship identification; (3) acquiring genetic information of a highly degraded material to be tested; (4) a progenitor source inference; (5) phenotype inference (inference of skin color).
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
SEQUENCE LISTING
<110> southern medical university, Guangzhou institute of criminal science and technology, Guangdong Yifu Biotechnology GmbH
<120> second generation sequencing detection system based on 224 InDel and 57 SNP sites
<130> 2022
<160> 562
<170> PatentIn version 3.5
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<211> 29
<212> DNA
<213> Artificial sequence
<400> 71
tgtcattgaa gatgtatcac acattctgt 29
<210> 72
<211> 32
<212> DNA
<213> Artificial sequence
<400> 72
gattttgtta agacatctca aagtctacct ct 32
<210> 73
<211> 35
<212> DNA
<213> Artificial sequence
<400> 73
tttgaataag ctgtaactca gtgtaactaa atgat 35
<210> 74
<211> 32
<212> DNA
<213> Artificial sequence
<400> 74
aggattcagg tgtttaatta gtcactttaa ga 32
<210> 75
<211> 25
<212> DNA
<213> Artificial sequence
<400> 75
ttatgggaat ccatagagca gtgag 25
<210> 76
<211> 26
<212> DNA
<213> Artificial sequence
<400> 76
gatatatatc ttgcagccca tctcct 26
<210> 77
<211> 22
<212> DNA
<213> Artificial sequence
<400> 77
gtgcacgcat gtccttttac tc 22
<210> 78
<211> 27
<212> DNA
<213> Artificial sequence
<400> 78
ttaccaactc attcaaaacc aaactgt 27
<210> 79
<211> 31
<212> DNA
<213> Artificial sequence
<400> 79
gtacacctta agtatccctc cttttacctt a 31
<210> 80
<211> 31
<212> DNA
<213> Artificial sequence
<400> 80
tgtatatact gtacagagat gcttcacctg a 31
<210> 81
<211> 29
<212> DNA
<213> Artificial sequence
<400> 81
ataactttaa cataagaagc gtggaagct 29
<210> 82
<211> 28
<212> DNA
<213> Artificial sequence
<400> 82
cctctgcaca taaagagaaa gttctcac 28
<210> 83
<211> 21
<212> DNA
<213> Artificial sequence
<400> 83
agctctgcaa gaatgtggaa g 21
<210> 84
<211> 27
<212> DNA
<213> Artificial sequence
<400> 84
atttatccac cgttatggtg tcttaca 27
<210> 85
<211> 28
<212> DNA
<213> Artificial sequence
<400> 85
gcaaagaaag aagacataaa cctgagtg 28
<210> 86
<211> 25
<212> DNA
<213> Artificial sequence
<400> 86
caacagagaa gtgaacctgt ttctg 25
<210> 87
<211> 22
<212> DNA
<213> Artificial sequence
<400> 87
gctcatgcaa ttgatcaaac ca 22
<210> 88
<211> 27
<212> DNA
<213> Artificial sequence
<400> 88
gctaccagca tttccaccaa ctactat 27
<210> 89
<211> 28
<212> DNA
<213> Artificial sequence
<400> 89
ataaagtatt tttgtgtcct tgggtcac 28
<210> 90
<211> 27
<212> DNA
<213> Artificial sequence
<400> 90
atggtaggta cccattttca catcttt 27
<210> 91
<211> 31
<212> DNA
<213> Artificial sequence
<400> 91
attgcattat aaaagttgta gagattctgg a 31
<210> 92
<211> 26
<212> DNA
<213> Artificial sequence
<400> 92
agaagtgtgg aaattctgga atggta 26
<210> 93
<211> 26
<212> DNA
<213> Artificial sequence
<400> 93
cacccagcac agttctgatg atataa 26
<210> 94
<211> 24
<212> DNA
<213> Artificial sequence
<400> 94
ttcaagccag ttgtaatcct ggtt 24
<210> 95
<211> 24
<212> DNA
<213> Artificial sequence
<400> 95
tttcacagac ccctgaggta gttc 24
<210> 96
<211> 26
<212> DNA
<213> Artificial sequence
<400> 96
cagaccattg ttctgatctc ttgaaa 26
<210> 97
<211> 27
<212> DNA
<213> Artificial sequence
<400> 97
tgacagagat tagttaggca tgaggag 27
<210> 98
<211> 27
<212> DNA
<213> Artificial sequence
<400> 98
gatattcaac agcgtgtcct tctaaga 27
<210> 99
<211> 29
<212> DNA
<213> Artificial sequence
<400> 99
taaagcccat acttgtattc agtttcctt 29
<210> 100
<211> 21
<212> DNA
<213> Artificial sequence
<400> 100
ggaagctgcg gcaaaaatta c 21
<210> 101
<211> 23
<212> DNA
<213> Artificial sequence
<400> 101
gagtgtggtc tgaggtgcat aca 23
<210> 102
<211> 26
<212> DNA
<213> Artificial sequence
<400> 102
aaatatgccc ttgattatgt tgcaat 26
<210> 103
<211> 27
<212> DNA
<213> Artificial sequence
<400> 103
cagagactga aggatgaagt ttgaaag 27
<210> 104
<211> 23
<212> DNA
<213> Artificial sequence
<400> 104
aggtgacacg tcaaaccaaa aac 23
<210> 105
<211> 26
<212> DNA
<213> Artificial sequence
<400> 105
ttagtaggga gatgaggagt gactgc 26
<210> 106
<211> 28
<212> DNA
<213> Artificial sequence
<400> 106
aaacagtatt gttgttttgc tgtgtgtt 28
<210> 107
<211> 25
<212> DNA
<213> Artificial sequence
<400> 107
attgagttct tatcgtgcca gacac 25
<210> 108
<211> 22
<212> DNA
<213> Artificial sequence
<400> 108
cccattcatc tgcaaacaac tc 22
<210> 109
<211> 21
<212> DNA
<213> Artificial sequence
<400> 109
gcacccgacc actctcactt c 21
<210> 110
<211> 20
<212> DNA
<213> Artificial sequence
<400> 110
<210> 111
<211> 28
<212> DNA
<213> Artificial sequence
<400> 111
ccatatttgt gctcctattc tctttctc 28
<210> 112
<211> 26
<212> DNA
<213> Artificial sequence
<400> 112
caaccaggta tgcagtaaca ttcctt 26
<210> 113
<211> 23
<212> DNA
<213> Artificial sequence
<400> 113
ataggacact ctatgccatg ctc 23
<210> 114
<211> 28
<212> DNA
<213> Artificial sequence
<400> 114
ttgctacact tattatctgc aaagtcca 28
<210> 115
<211> 28
<212> DNA
<213> Artificial sequence
<400> 115
ggattgggtc tcaactttct gataaatc 28
<210> 116
<211> 21
<212> DNA
<213> Artificial sequence
<400> 116
tgaatgcatc gcgaaatgaa c 21
<210> 117
<211> 26
<212> DNA
<213> Artificial sequence
<400> 117
attttaattt ctctttctgc gctcaa 26
<210> 118
<211> 31
<212> DNA
<213> Artificial sequence
<400> 118
gatatcatgt tgtaacaaac ctgtaagaag c 31
<210> 119
<211> 28
<212> DNA
<213> Artificial sequence
<400> 119
gtaaccatag caacaaagta agatgggt 28
<210> 120
<211> 30
<212> DNA
<213> Artificial sequence
<400> 120
taaggattcc tgctatagag atgtaaatgg 30
<210> 121
<211> 25
<212> DNA
<213> Artificial sequence
<400> 121
tggtgtcttt tcttgactct tcttc 25
<210> 122
<211> 23
<212> DNA
<213> Artificial sequence
<400> 122
aggcagtgtg gatggaatga ata 23
<210> 123
<211> 24
<212> DNA
<213> Artificial sequence
<400> 123
tgctaaaata ggacatgcac caca 24
<210> 124
<211> 24
<212> DNA
<213> Artificial sequence
<400> 124
tgctcacttt aagcccttag cagt 24
<210> 125
<211> 26
<212> DNA
<213> Artificial sequence
<400> 125
ttgcctctga ttgcagttaa atgata 26
<210> 126
<211> 24
<212> DNA
<213> Artificial sequence
<400> 126
ctggtattca tgacgctcct gtac 24
<210> 127
<211> 32
<212> DNA
<213> Artificial sequence
<400> 127
tatgtgtgtg tgtctttagg tgtagagtca ta 32
<210> 128
<211> 28
<212> DNA
<213> Artificial sequence
<400> 128
actatgattc atcatcagcc tatggttt 28
<210> 129
<211> 29
<212> DNA
<213> Artificial sequence
<400> 129
gctacaccac atacacatgt atacacagc 29
<210> 130
<211> 29
<212> DNA
<213> Artificial sequence
<400> 130
cattcataat cctatcaaag aagcattgt 29
<210> 131
<211> 25
<212> DNA
<213> Artificial sequence
<400> 131
ttaacacatg gtgagtcggt tttct 25
<210> 132
<211> 26
<212> DNA
<213> Artificial sequence
<400> 132
ttcgtttgat aaaagtccct ctttga 26
<210> 133
<211> 27
<212> DNA
<213> Artificial sequence
<400> 133
caatatacaa gcacaaatga acaaggg 27
<210> 134
<211> 28
<212> DNA
<213> Artificial sequence
<400> 134
tttgactctg taagtctgtg acctgatc 28
<210> 135
<211> 25
<212> DNA
<213> Artificial sequence
<400> 135
ataaaatgca aaactcggat gtacg 25
<210> 136
<211> 24
<212> DNA
<213> Artificial sequence
<400> 136
ctacaaaact tgccagcatt tgag 24
<210> 137
<211> 25
<212> DNA
<213> Artificial sequence
<400> 137
tttgaagaat ccagaaatca tccct 25
<210> 138
<211> 27
<212> DNA
<213> Artificial sequence
<400> 138
catgcaccac cttctgtatt tttgtat 27
<210> 139
<211> 28
<212> DNA
<213> Artificial sequence
<400> 139
gcgtggtgat tggaagttta gtttatta 28
<210> 140
<211> 24
<212> DNA
<213> Artificial sequence
<400> 140
cccagatctt aaactttccc ctga 24
<210> 141
<211> 28
<212> DNA
<213> Artificial sequence
<400> 141
ccagccttct ccttatattt cttaacca 28
<210> 142
<211> 27
<212> DNA
<213> Artificial sequence
<400> 142
gttgaaaggg caggtattag tgaaatt 27
<210> 143
<211> 24
<212> DNA
<213> Artificial sequence
<400> 143
tccatgatgt gactgatcac aatg 24
<210> 144
<211> 23
<212> DNA
<213> Artificial sequence
<400> 144
gagtttccat ggaggatgct tct 23
<210> 145
<211> 28
<212> DNA
<213> Artificial sequence
<400> 145
ttcatgtcaa tacctccagt gctagtaa 28
<210> 146
<211> 23
<212> DNA
<213> Artificial sequence
<400> 146
ggaggcaaaa cctaataggg aca 23
<210> 147
<211> 25
<212> DNA
<213> Artificial sequence
<400> 147
gctctctaga ggtgagtaaa tggca 25
<210> 148
<211> 25
<212> DNA
<213> Artificial sequence
<400> 148
aggagcagag agtgattcac catta 25
<210> 149
<211> 27
<212> DNA
<213> Artificial sequence
<400> 149
cacacattaa aattctctcc cctttgt 27
<210> 150
<211> 26
<212> DNA
<213> Artificial sequence
<400> 150
tcattgtgtt aacttacttg ggaaag 26
<210> 151
<211> 32
<212> DNA
<213> Artificial sequence
<400> 151
acttttgtat gatcaacttg tgtctgaata ct 32
<210> 152
<211> 26
<212> DNA
<213> Artificial sequence
<400> 152
cctctgtgat caacagacaa tgatgt 26
<210> 153
<211> 22
<212> DNA
<213> Artificial sequence
<400> 153
ataaaacgca aatggtcctt gc 22
<210> 154
<211> 28
<212> DNA
<213> Artificial sequence
<400> 154
agaattgatt acactatagg agccctga 28
<210> 155
<211> 23
<212> DNA
<213> Artificial sequence
<400> 155
ggagtctgaa gatgtgacaa ccg 23
<210> 156
<211> 28
<212> DNA
<213> Artificial sequence
<400> 156
tcactgcact tcaagagata ttcattgt 28
<210> 157
<211> 24
<212> DNA
<213> Artificial sequence
<400> 157
caaagagaaa actggcaaat cagc 24
<210> 158
<211> 26
<212> DNA
<213> Artificial sequence
<400> 158
attataatgt cccacctgaa ctcttc 26
<210> 159
<211> 28
<212> DNA
<213> Artificial sequence
<400> 159
gggaatcact gaattacctg agaactac 28
<210> 160
<211> 32
<212> DNA
<213> Artificial sequence
<400> 160
taataagcag tatgaacaac aaaactaaag ca 32
<210> 161
<211> 22
<212> DNA
<213> Artificial sequence
<400> 161
atcaaagcag aatggctcca ac 22
<210> 162
<211> 24
<212> DNA
<213> Artificial sequence
<400> 162
acttcaggtc aaatgcctga ttgt 24
<210> 163
<211> 21
<212> DNA
<213> Artificial sequence
<400> 163
atggcaacct gcttcatgtg t 21
<210> 164
<211> 25
<212> DNA
<213> Artificial sequence
<400> 164
gtcaatcatg aaatcacttc aaggg 25
<210> 165
<211> 26
<212> DNA
<213> Artificial sequence
<400> 165
caatgtgtgt aggtgaacta actgcc 26
<210> 166
<211> 27
<212> DNA
<213> Artificial sequence
<400> 166
catgtacatc agaacagctg ctacttg 27
<210> 167
<211> 24
<212> DNA
<213> Artificial sequence
<400> 167
taatcagagg ctgaagtgaa acca 24
<210> 168
<211> 26
<212> DNA
<213> Artificial sequence
<400> 168
cacggattta ttgaaaccaa agtatg 26
<210> 169
<211> 24
<212> DNA
<213> Artificial sequence
<400> 169
gagcttcagg tatgccacat agtg 24
<210> 170
<211> 25
<212> DNA
<213> Artificial sequence
<400> 170
gagaaaacct tgtgagctga gaaag 25
<210> 171
<211> 27
<212> DNA
<213> Artificial sequence
<400> 171
atgaggatga caaaacctat ctcactg 27
<210> 172
<211> 29
<212> DNA
<213> Artificial sequence
<400> 172
atgtctccac accattactg aatattgat 29
<210> 173
<211> 30
<212> DNA
<213> Artificial sequence
<400> 173
taatattgtc agtagcactt gggtactcaa 30
<210> 174
<211> 20
<212> DNA
<213> Artificial sequence
<400> 174
<210> 175
<211> 29
<212> DNA
<213> Artificial sequence
<400> 175
cataagaaat ctggcttatc cactaccat 29
<210> 176
<211> 26
<212> DNA
<213> Artificial sequence
<400> 176
ttacagatta caagctgatt cagttg 26
<210> 177
<211> 28
<212> DNA
<213> Artificial sequence
<400> 177
ccagcttaga atgatgaaaa gttctctt 28
<210> 178
<211> 25
<212> DNA
<213> Artificial sequence
<400> 178
cacaggagaa ggatttcaga cctac 25
<210> 179
<211> 21
<212> DNA
<213> Artificial sequence
<400> 179
aagtgcagga atgcatatcg g 21
<210> 180
<211> 23
<212> DNA
<213> Artificial sequence
<400> 180
agaagtaaat gagggcacct tcc 23
<210> 181
<211> 26
<212> DNA
<213> Artificial sequence
<400> 181
agcttcctca caacaaaatg tgatag 26
<210> 182
<211> 28
<212> DNA
<213> Artificial sequence
<400> 182
attccaacta agaacacatc agtgtcag 28
<210> 183
<211> 23
<212> DNA
<213> Artificial sequence
<400> 183
cattgtgcat catttgccag taa 23
<210> 184
<211> 24
<212> DNA
<213> Artificial sequence
<400> 184
ccacactatg agcagccaca ctac 24
<210> 185
<211> 33
<212> DNA
<213> Artificial sequence
<400> 185
gcaagactcc atctcaaata atagtaataa tca 33
<210> 186
<211> 27
<212> DNA
<213> Artificial sequence
<400> 186
caagacacat atatggaaag agggtgt 27
<210> 187
<211> 28
<212> DNA
<213> Artificial sequence
<400> 187
atactggtgg agtcctattc ctaagtgt 28
<210> 188
<211> 24
<212> DNA
<213> Artificial sequence
<400> 188
gatggatgca gttggatgaa actt 24
<210> 189
<211> 28
<212> DNA
<213> Artificial sequence
<400> 189
taaccagttc tttaagacga atttgttt 28
<210> 190
<211> 26
<212> DNA
<213> Artificial sequence
<400> 190
catgtcaatg cattacatat ctccca 26
<210> 191
<211> 28
<212> DNA
<213> Artificial sequence
<400> 191
tctgaacact tttctaccga agaaactt 28
<210> 192
<211> 24
<212> DNA
<213> Artificial sequence
<400> 192
cagggccata tggttaatct agca 24
<210> 193
<211> 25
<212> DNA
<213> Artificial sequence
<400> 193
ttcctaaagg actccaaaag ggatt 25
<210> 194
<211> 25
<212> DNA
<213> Artificial sequence
<400> 194
ggaaaagcaa tgcttctctg gtagt 25
<210> 195
<211> 26
<212> DNA
<213> Artificial sequence
<400> 195
cagcacactt tagacacatg cctatt 26
<210> 196
<211> 26
<212> DNA
<213> Artificial sequence
<400> 196
cagccctgtt tcattaattt ttgaat 26
<210> 197
<211> 27
<212> DNA
<213> Artificial sequence
<400> 197
ggaggcttga actacttgag atcagta 27
<210> 198
<211> 30
<212> DNA
<213> Artificial sequence
<400> 198
taccacttcg cagtgttgtt attattatgt 30
<210> 199
<211> 28
<212> DNA
<213> Artificial sequence
<400> 199
gtagatgtca ggtcttccct agtgaaaa 28
<210> 200
<211> 25
<212> DNA
<213> Artificial sequence
<400> 200
ttcagtgcat ttcatgagga aaatt 25
<210> 201
<211> 29
<212> DNA
<213> Artificial sequence
<400> 201
taactatcac tttttgaact gccagtacc 29
<210> 202
<211> 32
<212> DNA
<213> Artificial sequence
<400> 202
tactatccta tgcaagttgt ttacagtgtt tc 32
<210> 203
<211> 26
<212> DNA
<213> Artificial sequence
<400> 203
aagatcatac tttgaggttc tggcaa 26
<210> 204
<211> 23
<212> DNA
<213> Artificial sequence
<400> 204
gcatcgtgaa ttgattcttg agc 23
<210> 205
<211> 25
<212> DNA
<213> Artificial sequence
<400> 205
aatgacttaa accttggact gggac 25
<210> 206
<211> 25
<212> DNA
<213> Artificial sequence
<400> 206
accaaaattc ttccactcca aagac 25
<210> 207
<211> 30
<212> DNA
<213> Artificial sequence
<400> 207
gacacagcca aatcatgtca attataatac 30
<210> 208
<211> 29
<212> DNA
<213> Artificial sequence
<400> 208
ggcaatttta ttgtgcaaat atcagagta 29
<210> 209
<211> 27
<212> DNA
<213> Artificial sequence
<400> 209
ctcacatgtt atttttcttg gacagaa 27
<210> 210
<211> 29
<212> DNA
<213> Artificial sequence
<400> 210
aaccctacaa tagcacctaa tttatcagc 29
<210> 211
<211> 28
<212> DNA
<213> Artificial sequence
<400> 211
cgtctgaagg attttcttta gtgtttct 28
<210> 212
<211> 20
<212> DNA
<213> Artificial sequence
<400> 212
<210> 213
<211> 25
<212> DNA
<213> Artificial sequence
<400> 213
gctcagatgt tgaaacaacc ctagg 25
<210> 214
<211> 30
<212> DNA
<213> Artificial sequence
<400> 214
aattaaaagt ttatctagct ctcctgccat 30
<210> 215
<211> 28
<212> DNA
<213> Artificial sequence
<400> 215
tgaagaaacc tgcagataaa ctacacag 28
<210> 216
<211> 27
<212> DNA
<213> Artificial sequence
<400> 216
ctgtatcgtt atatttggtt gcatttc 27
<210> 217
<211> 29
<212> DNA
<213> Artificial sequence
<400> 217
cagtatatgc atgtctgtac aggtgaaat 29
<210> 218
<211> 30
<212> DNA
<213> Artificial sequence
<400> 218
taaaaaagag gatactagac tggaaagggt 30
<210> 219
<211> 23
<212> DNA
<213> Artificial sequence
<400> 219
ccatctggct atgcacgttt aca 23
<210> 220
<211> 26
<212> DNA
<213> Artificial sequence
<400> 220
attatgttct tctaccagca gttgcc 26
<210> 221
<211> 27
<212> DNA
<213> Artificial sequence
<400> 221
gctgtgtaag gacactgttt tgagatc 27
<210> 222
<211> 25
<212> DNA
<213> Artificial sequence
<400> 222
ctgcgtttcc ctaatgtttt acaat 25
<210> 223
<211> 24
<212> DNA
<213> Artificial sequence
<400> 223
gctcatgatg ttgcttaaag ctca 24
<210> 224
<211> 25
<212> DNA
<213> Artificial sequence
<400> 224
tgctttgttc attggtttgg tttta 25
<210> 225
<211> 29
<212> DNA
<213> Artificial sequence
<400> 225
acatacacac acaagtaaat gaaaaggaa 29
<210> 226
<211> 27
<212> DNA
<213> Artificial sequence
<400> 226
atatgttgta acatgtcacc tgtggtc 27
<210> 227
<211> 21
<212> DNA
<213> Artificial sequence
<400> 227
tcaactgtgt gagccaacga a 21
<210> 228
<211> 25
<212> DNA
<213> Artificial sequence
<400> 228
cgtgcctggc ctattttagt taggt 25
<210> 229
<211> 26
<212> DNA
<213> Artificial sequence
<400> 229
agcaaaatag agtggaattc accagt 26
<210> 230
<211> 28
<212> DNA
<213> Artificial sequence
<400> 230
tggtgcttac tacaagagca aaatcttt 28
<210> 231
<211> 22
<212> DNA
<213> Artificial sequence
<400> 231
ttgaggttgg gagttcgaca ac 22
<210> 232
<211> 29
<212> DNA
<213> Artificial sequence
<400> 232
gcagataaga aaatcaaagc aaaagagaa 29
<210> 233
<211> 23
<212> DNA
<213> Artificial sequence
<400> 233
ttagtgatgc acaaagccag tca 23
<210> 234
<211> 29
<212> DNA
<213> Artificial sequence
<400> 234
aagatcactg cataattcat gtctaggaa 29
<210> 235
<211> 28
<212> DNA
<213> Artificial sequence
<400> 235
gaaaagaggt ttaattgact cacgtctc 28
<210> 236
<211> 22
<212> DNA
<213> Artificial sequence
<400> 236
cactgcactc cagtgagatt cc 22
<210> 237
<211> 22
<212> DNA
<213> Artificial sequence
<400> 237
gggcattcca tttgaagata gc 22
<210> 238
<211> 25
<212> DNA
<213> Artificial sequence
<400> 238
cttgatttga agctgacaac cattt 25
<210> 239
<211> 26
<212> DNA
<213> Artificial sequence
<400> 239
attcaacatg ctaaaaacaa acccat 26
<210> 240
<211> 23
<212> DNA
<213> Artificial sequence
<400> 240
accaacagtg ggtatgtagc gtt 23
<210> 241
<211> 29
<212> DNA
<213> Artificial sequence
<400> 241
agagactatc ctttccctat tgctttctt 29
<210> 242
<211> 29
<212> DNA
<213> Artificial sequence
<400> 242
caatcctaat atatgtggaa ctgcaaaag 29
<210> 243
<211> 23
<212> DNA
<213> Artificial sequence
<400> 243
cctgggcaca aagttcattt tct 23
<210> 244
<211> 24
<212> DNA
<213> Artificial sequence
<400> 244
cctgcatcca aaatctttgc ttag 24
<210> 245
<211> 27
<212> DNA
<213> Artificial sequence
<400> 245
atagctaacc tccattgaga gaatgct 27
<210> 246
<211> 23
<212> DNA
<213> Artificial sequence
<400> 246
tcaagaatat gaaaagcgct cca 23
<210> 247
<211> 31
<212> DNA
<213> Artificial sequence
<400> 247
aatactaacc tcaaatgaaa atgggataaa t 31
<210> 248
<211> 26
<212> DNA
<213> Artificial sequence
<400> 248
ccattatgta atgcccttct tctttg 26
<210> 249
<211> 23
<212> DNA
<213> Artificial sequence
<400> 249
tcttggctga catttttggc taa 23
<210> 250
<211> 25
<212> DNA
<213> Artificial sequence
<400> 250
gagattcaaa gccagcttct cttaa 25
<210> 251
<211> 30
<212> DNA
<213> Artificial sequence
<400> 251
gtctgtacat aatctacatc ttccagtgct 30
<210> 252
<211> 28
<212> DNA
<213> Artificial sequence
<400> 252
tggggactac tatagtagcc agtgtacg 28
<210> 253
<211> 22
<212> DNA
<213> Artificial sequence
<400> 253
gggaccatat ttggactctg ca 22
<210> 254
<211> 22
<212> DNA
<213> Artificial sequence
<400> 254
tcagggagag ggtcttctgg at 22
<210> 255
<211> 21
<212> DNA
<213> Artificial sequence
<400> 255
atgggggaca gtgtgaggta c 21
<210> 256
<211> 19
<212> DNA
<213> Artificial sequence
<400> 256
ccacctgtcc cggacacta 19
<210> 257
<211> 22
<212> DNA
<213> Artificial sequence
<400> 257
gctggaacac agaatgcact gt 22
<210> 258
<211> 27
<212> DNA
<213> Artificial sequence
<400> 258
ctatctaagg agcatgttca gaaacca 27
<210> 259
<211> 23
<212> DNA
<213> Artificial sequence
<400> 259
aggcgctggg tatgtaaaaa gtc 23
<210> 260
<211> 24
<212> DNA
<213> Artificial sequence
<400> 260
tgatgccacc ttgacaacat aagt 24
<210> 261
<211> 27
<212> DNA
<213> Artificial sequence
<400> 261
tagacagaga tgacaggtag atggagg 27
<210> 262
<211> 32
<212> DNA
<213> Artificial sequence
<400> 262
ctaatctatc catctatcat ctctatccat cc 32
<210> 263
<211> 23
<212> DNA
<213> Artificial sequence
<400> 263
aaccttgaaa accatgcaag tga 23
<210> 264
<211> 23
<212> DNA
<213> Artificial sequence
<400> 264
cccatttaaa gggcacattc agt 23
<210> 265
<211> 25
<212> DNA
<213> Artificial sequence
<400> 265
tggcctatca gagacaatta tacca 25
<210> 266
<211> 28
<212> DNA
<213> Artificial sequence
<400> 266
tgctttaagt gttatgttga gacaccag 28
<210> 267
<211> 26
<212> DNA
<213> Artificial sequence
<400> 267
aacaactaga aaaatccaaa tgcctg 26
<210> 268
<211> 24
<212> DNA
<213> Artificial sequence
<400> 268
aaaatcctta tgccgaacac agag 24
<210> 269
<211> 24
<212> DNA
<213> Artificial sequence
<400> 269
tggctttgcc tttgtttgaa tatc 24
<210> 270
<211> 17
<212> DNA
<213> Artificial sequence
<400> 270
gccagtaatt ggagcag 17
<210> 271
<211> 23
<212> DNA
<213> Artificial sequence
<400> 271
agggttcact gcagcctcaa tat 23
<210> 272
<211> 28
<212> DNA
<213> Artificial sequence
<400> 272
ggaacctagt aaatgcttta ttagggtt 28
<210> 273
<211> 29
<212> DNA
<213> Artificial sequence
<400> 273
ccagaggtct tgataacatg taaagagtt 29
<210> 274
<211> 25
<212> DNA
<213> Artificial sequence
<400> 274
gaccaatgcc tcaaggagtt atagg 25
<210> 275
<211> 24
<212> DNA
<213> Artificial sequence
<400> 275
gagaaaagca gctcaggaac attc 24
<210> 276
<211> 24
<212> DNA
<213> Artificial sequence
<400> 276
gggtaggaag agtctcattt gggt 24
<210> 277
<211> 24
<212> DNA
<213> Artificial sequence
<400> 277
ggacaatgct ctgttcttac tgga 24
<210> 278
<211> 27
<212> DNA
<213> Artificial sequence
<400> 278
gatgacttgc atatttttgt cttcagc 27
<210> 279
<211> 25
<212> DNA
<213> Artificial sequence
<400> 279
tgccttctgg agataactga gtcct 25
<210> 280
<211> 23
<212> DNA
<213> Artificial sequence
<400> 280
tggaggattc caggaaaaca ctc 23
<210> 281
<211> 23
<212> DNA
<213> Artificial sequence
<400> 281
cctgcagcct tgatgaaata ctt 23
<210> 282
<211> 26
<212> DNA
<213> Artificial sequence
<400> 282
accttaggca atttgcatta atatgg 26
<210> 283
<211> 26
<212> DNA
<213> Artificial sequence
<400> 283
ctgaaaatga gagcctagag atcacc 26
<210> 284
<211> 25
<212> DNA
<213> Artificial sequence
<400> 284
ctccaatttc ttcctcaaaa ggtct 25
<210> 285
<211> 26
<212> DNA
<213> Artificial sequence
<400> 285
cttctgacaa aaacctagct ctggac 26
<210> 286
<211> 29
<212> DNA
<213> Artificial sequence
<400> 286
ccatagctaa cactcaacac tcctcttag 29
<210> 287
<211> 23
<212> DNA
<213> Artificial sequence
<400> 287
cttcgatttc acttatctcg tgg 23
<210> 288
<211> 25
<212> DNA
<213> Artificial sequence
<400> 288
tcatgccacc acttttctct ttaac 25
<210> 289
<211> 26
<212> DNA
<213> Artificial sequence
<400> 289
cacacttgaa tgtggatctg agtgac 26
<210> 290
<211> 23
<212> DNA
<213> Artificial sequence
<400> 290
aaggaggcac cacaggtcta tgt 23
<210> 291
<211> 28
<212> DNA
<213> Artificial sequence
<400> 291
tcattctccc tttcttatgc aaaaagta 28
<210> 292
<211> 23
<212> DNA
<213> Artificial sequence
<400> 292
gggatacaga gtccctccta ggc 23
<210> 293
<211> 27
<212> DNA
<213> Artificial sequence
<400> 293
aattaaagtg tagatttttc aaaagca 27
<210> 294
<211> 19
<212> DNA
<213> Artificial sequence
<400> 294
cccagcattt ggaagttgc 19
<210> 295
<211> 26
<212> DNA
<213> Artificial sequence
<400> 295
gggtagattt tagctattcg tcacca 26
<210> 296
<211> 25
<212> DNA
<213> Artificial sequence
<400> 296
gaggttgcca aactaaggct agttg 25
<210> 297
<211> 23
<212> DNA
<213> Artificial sequence
<400> 297
gggatgccat aggaattctt acc 23
<210> 298
<211> 25
<212> DNA
<213> Artificial sequence
<400> 298
gggccaattc tgtgtaagat ttgat 25
<210> 299
<211> 24
<212> DNA
<213> Artificial sequence
<400> 299
gggaagctga agggtgagaa ttac 24
<210> 300
<211> 25
<212> DNA
<213> Artificial sequence
<400> 300
cgtgaggata ctggtggaga tccta 25
<210> 301
<211> 23
<212> DNA
<213> Artificial sequence
<400> 301
ccctgagacc cagatatcag acc 23
<210> 302
<211> 24
<212> DNA
<213> Artificial sequence
<400> 302
tccctgctct atcattccaa agag 24
<210> 303
<211> 28
<212> DNA
<213> Artificial sequence
<400> 303
gagaagatgt gcaaatcatt cacaaata 28
<210> 304
<211> 25
<212> DNA
<213> Artificial sequence
<400> 304
ggtaaaggct gcagtgattg agact 25
<210> 305
<211> 29
<212> DNA
<213> Artificial sequence
<400> 305
tatcacatca ctgacacaca agaatacct 29
<210> 306
<211> 25
<212> DNA
<213> Artificial sequence
<400> 306
cagtggcagg tgttcaacaa ttaac 25
<210> 307
<211> 26
<212> DNA
<213> Artificial sequence
<400> 307
ataaaggtac agataaggcc caaagg 26
<210> 308
<211> 29
<212> DNA
<213> Artificial sequence
<400> 308
aagctctact atcttcagga caaggaaaa 29
<210> 309
<211> 22
<212> DNA
<213> Artificial sequence
<400> 309
cctgatacat gagcaatttg gg 22
<210> 310
<211> 26
<212> DNA
<213> Artificial sequence
<400> 310
tgttttgtaa gccatttgct gtaaga 26
<210> 311
<211> 31
<212> DNA
<213> Artificial sequence
<400> 311
attgattctt ctatctccca gggatatact a 31
<210> 312
<211> 26
<212> DNA
<213> Artificial sequence
<400> 312
gcatgtctat aattgtctac cccgaa 26
<210> 313
<211> 26
<212> DNA
<213> Artificial sequence
<400> 313
cctaggctgg tctctaagtc ctgaac 26
<210> 314
<211> 26
<212> DNA
<213> Artificial sequence
<400> 314
tgtgtgtgtg tgtattgctg taagga 26
<210> 315
<211> 24
<212> DNA
<213> Artificial sequence
<400> 315
ttcaggtcta catgtaggat cggg 24
<210> 316
<211> 28
<212> DNA
<213> Artificial sequence
<400> 316
gttcctgagc atgtctagta gagaaggt 28
<210> 317
<211> 17
<212> DNA
<213> Artificial sequence
<400> 317
ggcacgcgga ttgagag 17
<210> 318
<211> 22
<212> DNA
<213> Artificial sequence
<400> 318
ttctgctccg taagtggaaa gg 22
<210> 319
<211> 23
<212> DNA
<213> Artificial sequence
<400> 319
gtagtgggaa gatggccaca taa 23
<210> 320
<211> 26
<212> DNA
<213> Artificial sequence
<400> 320
aactggaatt atttcatggg acagtg 26
<210> 321
<211> 27
<212> DNA
<213> Artificial sequence
<400> 321
taggaccact cctcaataaa catacca 27
<210> 322
<211> 23
<212> DNA
<213> Artificial sequence
<400> 322
gtgggtaggc agtagtactt cca 23
<210> 323
<211> 21
<212> DNA
<213> Artificial sequence
<400> 323
aaagaggaag attggtttcc g 21
<210> 324
<211> 21
<212> DNA
<213> Artificial sequence
<400> 324
cccttccatc ctatactgac g 21
<210> 325
<211> 25
<212> DNA
<213> Artificial sequence
<400> 325
ccctctctct cactcggtct actct 25
<210> 326
<211> 27
<212> DNA
<213> Artificial sequence
<400> 326
agagcaagtc tctcattcat attcagg 27
<210> 327
<211> 25
<212> DNA
<213> Artificial sequence
<400> 327
ccaccagagc actacagcct tttat 25
<210> 328
<211> 26
<212> DNA
<213> Artificial sequence
<400> 328
ctcataccac cacctgcagt tagtta 26
<210> 329
<211> 27
<212> DNA
<213> Artificial sequence
<400> 329
caggttttaa aaatatcctg ctggttg 27
<210> 330
<211> 24
<212> DNA
<213> Artificial sequence
<400> 330
aaacaacaaa atccccagtc tgtc 24
<210> 331
<211> 28
<212> DNA
<213> Artificial sequence
<400> 331
gtaatagagt gagccttcat ccctacaa 28
<210> 332
<211> 26
<212> DNA
<213> Artificial sequence
<400> 332
tgcacctggc ctatattttc taactc 26
<210> 333
<211> 30
<212> DNA
<213> Artificial sequence
<400> 333
atattattct ggttgcatgt cctgtaagta 30
<210> 334
<211> 25
<212> DNA
<213> Artificial sequence
<400> 334
ctggtgcttt tatggttttc tttcc 25
<210> 335
<211> 20
<212> DNA
<213> Artificial sequence
<400> 335
catttgcttg gagcccaatc 20
<210> 336
<211> 22
<212> DNA
<213> Artificial sequence
<400> 336
ggttacataa acccatccaa gc 22
<210> 337
<211> 27
<212> DNA
<213> Artificial sequence
<400> 337
atagtatggg tcaatcttgt gttcctg 27
<210> 338
<211> 24
<212> DNA
<213> Artificial sequence
<400> 338
cactccagca tttcaggttc tgat 24
<210> 339
<211> 25
<212> DNA
<213> Artificial sequence
<400> 339
gtgtgctggt ccattctctt gtaac 25
<210> 340
<211> 24
<212> DNA
<213> Artificial sequence
<400> 340
attctctccc tctggtgatt tcct 24
<210> 341
<211> 25
<212> DNA
<213> Artificial sequence
<400> 341
ggtgccatac tttctgcatt tttct 25
<210> 342
<211> 26
<212> DNA
<213> Artificial sequence
<400> 342
ccgactaaac tttgacacaa cagaga 26
<210> 343
<211> 34
<212> DNA
<213> Artificial sequence
<400> 343
agtgacatta aatttgtata catttctcct gtta 34
<210> 344
<211> 26
<212> DNA
<213> Artificial sequence
<400> 344
tttttgcaga gacacaatct ccctat 26
<210> 345
<211> 22
<212> DNA
<213> Artificial sequence
<400> 345
gcctcttcgt ctcatcgaca ct 22
<210> 346
<211> 19
<212> DNA
<213> Artificial sequence
<400> 346
gccgtgatac ctggcgttt 19
<210> 347
<211> 28
<212> DNA
<213> Artificial sequence
<400> 347
ctcagattaa ccatctaata tcgcacat 28
<210> 348
<211> 25
<212> DNA
<213> Artificial sequence
<400> 348
ggggtccctt attacaagtg acaca 25
<210> 349
<211> 27
<212> DNA
<213> Artificial sequence
<400> 349
gtgtgtggtt acaggaataa gcagttc 27
<210> 350
<211> 22
<212> DNA
<213> Artificial sequence
<400> 350
ctggttcacg aagccacaag ta 22
<210> 351
<211> 26
<212> DNA
<213> Artificial sequence
<400> 351
atgcaggtaa ggttaggctg agatta 26
<210> 352
<211> 31
<212> DNA
<213> Artificial sequence
<400> 352
gaaactaaac tcagtaggtt aacttacttg c 31
<210> 353
<211> 25
<212> DNA
<213> Artificial sequence
<400> 353
gctttgcctg gaaacttgaa tctaa 25
<210> 354
<211> 27
<212> DNA
<213> Artificial sequence
<400> 354
gctctacaga aactccctga cttcatc 27
<210> 355
<211> 27
<212> DNA
<213> Artificial sequence
<400> 355
catggagact tagcattgaa tcacttt 27
<210> 356
<211> 29
<212> DNA
<213> Artificial sequence
<400> 356
ggctgagaaa cacacactta actgataaa 29
<210> 357
<211> 25
<212> DNA
<213> Artificial sequence
<400> 357
ccatcaccac aatcattatt atcgc 25
<210> 358
<211> 25
<212> DNA
<213> Artificial sequence
<400> 358
gccaaagaac acaatcaatc aaaat 25
<210> 359
<211> 28
<212> DNA
<213> Artificial sequence
<400> 359
caggagaaat gcatgtgtta ggattatt 28
<210> 360
<211> 24
<212> DNA
<213> Artificial sequence
<400> 360
gcaggatttt gaactgaaat caca 24
<210> 361
<211> 25
<212> DNA
<213> Artificial sequence
<400> 361
gagggagatc agacgttttt gtttc 25
<210> 362
<211> 24
<212> DNA
<213> Artificial sequence
<400> 362
tgcaaaataa gagagctggg cata 24
<210> 363
<211> 26
<212> DNA
<213> Artificial sequence
<400> 363
gatgaatcat gctttggaaa gagaac 26
<210> 364
<211> 26
<212> DNA
<213> Artificial sequence
<400> 364
caccaggaaa atagcaaatg ttcata 26
<210> 365
<211> 27
<212> DNA
<213> Artificial sequence
<400> 365
ggtgcctaat ttatgaccca ctcataa 27
<210> 366
<211> 24
<212> DNA
<213> Artificial sequence
<400> 366
caaacatttt tcaagggcaa tgat 24
<210> 367
<211> 21
<212> DNA
<213> Artificial sequence
<400> 367
ttccccaaga tcacacacca a 21
<210> 368
<211> 25
<212> DNA
<213> Artificial sequence
<400> 368
agggaggaga atttagtgtg gaaaa 25
<210> 369
<211> 23
<212> DNA
<213> Artificial sequence
<400> 369
agttaactgg gtgaccgatc ctc 23
<210> 370
<211> 25
<212> DNA
<213> Artificial sequence
<400> 370
catgaactga cagtcaagta ggcaa 25
<210> 371
<211> 23
<212> DNA
<213> Artificial sequence
<400> 371
ccaacttagg tcaccacatt tcc 23
<210> 372
<211> 26
<212> DNA
<213> Artificial sequence
<400> 372
tgatggccta cttagtcaca aatcct 26
<210> 373
<211> 23
<212> DNA
<213> Artificial sequence
<400> 373
tgacgatggt cgtcctagat gac 23
<210> 374
<211> 28
<212> DNA
<213> Artificial sequence
<400> 374
taattggctg tttctgttga gatgtaga 28
<210> 375
<211> 26
<212> DNA
<213> Artificial sequence
<400> 375
ggctgggact tttgtaagtc aattag 26
<210> 376
<211> 26
<212> DNA
<213> Artificial sequence
<400> 376
atgcaactag gccaatacac ctaaac 26
<210> 377
<211> 29
<212> DNA
<213> Artificial sequence
<400> 377
gctcttagtg attgtgtcct tatgagatt 29
<210> 378
<211> 22
<212> DNA
<213> Artificial sequence
<400> 378
attatgcagc cccaccatct aa 22
<210> 379
<211> 27
<212> DNA
<213> Artificial sequence
<400> 379
ccaacaaaat ccattctgga ataagat 27
<210> 380
<211> 23
<212> DNA
<213> Artificial sequence
<400> 380
tgcaccttct accatgagtg gat 23
<210> 381
<211> 29
<212> DNA
<213> Artificial sequence
<400> 381
agcttgaaaa atacaccaaa gtctgagta 29
<210> 382
<211> 24
<212> DNA
<213> Artificial sequence
<400> 382
tttcctgctt gtatttcctg cagt 24
<210> 383
<211> 28
<212> DNA
<213> Artificial sequence
<400> 383
gtaaaactgt agttggagat gcaatgaa 28
<210> 384
<211> 22
<212> DNA
<213> Artificial sequence
<400> 384
gtaaatgagc cagccatggg ac 22
<210> 385
<211> 26
<212> DNA
<213> Artificial sequence
<400> 385
aaattgaatc agtggaagct tgctaa 26
<210> 386
<211> 32
<212> DNA
<213> Artificial sequence
<400> 386
tagaatagtg acttcctatg tacccaaaac tc 32
<210> 387
<211> 27
<212> DNA
<213> Artificial sequence
<400> 387
tccatgaggt caactcaaaa gagtaga 27
<210> 388
<211> 26
<212> DNA
<213> Artificial sequence
<400> 388
tgaaacctta tctccacgaa aaatca 26
<210> 389
<211> 28
<212> DNA
<213> Artificial sequence
<400> 389
ttgttccact ctacaaatta ggcacata 28
<210> 390
<211> 26
<212> DNA
<213> Artificial sequence
<400> 390
gatgtaactc catctgataa ccctag 26
<210> 391
<211> 24
<212> DNA
<213> Artificial sequence
<400> 391
cctcagcctc cagaacaatt aaga 24
<210> 392
<211> 24
<212> DNA
<213> Artificial sequence
<400> 392
ggaaacttca gacgctcaac agat 24
<210> 393
<211> 22
<212> DNA
<213> Artificial sequence
<400> 393
aacccaatac atgctttcac cc 22
<210> 394
<211> 24
<212> DNA
<213> Artificial sequence
<400> 394
ccttaatgct attagggcaa aaat 24
<210> 395
<211> 30
<212> DNA
<213> Artificial sequence
<400> 395
gagtgaggaa acatctgttt atactttgct 30
<210> 396
<211> 30
<212> DNA
<213> Artificial sequence
<400> 396
taatacatgt agcagaatct ctggaaacac 30
<210> 397
<211> 27
<212> DNA
<213> Artificial sequence
<400> 397
ctgccataaa cctatgagca ggtaata 27
<210> 398
<211> 26
<212> DNA
<213> Artificial sequence
<400> 398
ggttatcagc ctgacttgtc atatcc 26
<210> 399
<211> 28
<212> DNA
<213> Artificial sequence
<400> 399
tgaaataatg tggtataact tctctgga 28
<210> 400
<211> 27
<212> DNA
<213> Artificial sequence
<400> 400
aaaggcagca gtttgaatca ttataca 27
<210> 401
<211> 27
<212> DNA
<213> Artificial sequence
<400> 401
tctaagagtc tgtagaatgg agaggca 27
<210> 402
<211> 30
<212> DNA
<213> Artificial sequence
<400> 402
atttcactgt tttctttagt ctgttcactg 30
<210> 403
<211> 24
<212> DNA
<213> Artificial sequence
<400> 403
ttccaaaatt tgtgttgaag ctga 24
<210> 404
<211> 26
<212> DNA
<213> Artificial sequence
<400> 404
atttattttc tacagccctg aagcct 26
<210> 405
<211> 27
<212> DNA
<213> Artificial sequence
<400> 405
cccatttgtg ataatctgct ttactgt 27
<210> 406
<211> 25
<212> DNA
<213> Artificial sequence
<400> 406
ccagccaact gttgagtttt aatgt 25
<210> 407
<211> 34
<212> DNA
<213> Artificial sequence
<400> 407
aatatagtag gcagtattta cagggttatc caat 34
<210> 408
<211> 20
<212> DNA
<213> Artificial sequence
<400> 408
<210> 409
<211> 27
<212> DNA
<213> Artificial sequence
<400> 409
tggctgtgtc ttactcaaaa tcaactt 27
<210> 410
<211> 28
<212> DNA
<213> Artificial sequence
<400> 410
tatgacaata cattttcatc gcaacagt 28
<210> 411
<211> 22
<212> DNA
<213> Artificial sequence
<400> 411
ggccatgctt aaagcttatc gc 22
<210> 412
<211> 25
<212> DNA
<213> Artificial sequence
<400> 412
tttaaagagg aatgcttctg ttccc 25
<210> 413
<211> 27
<212> DNA
<213> Artificial sequence
<400> 413
acactttaat tttgagcatt tccagtg 27
<210> 414
<211> 22
<212> DNA
<213> Artificial sequence
<400> 414
aatgtgaatg cctgctaatg ca 22
<210> 415
<211> 28
<212> DNA
<213> Artificial sequence
<400> 415
gctcgactat ttagagatca caacaaaa 28
<210> 416
<211> 26
<212> DNA
<213> Artificial sequence
<400> 416
cctgctctaa ttctcccact ttacct 26
<210> 417
<211> 29
<212> DNA
<213> Artificial sequence
<400> 417
gccttataac tattggctac aggctatca 29
<210> 418
<211> 23
<212> DNA
<213> Artificial sequence
<400> 418
ctgtcattag ctcgctgttc atg 23
<210> 419
<211> 28
<212> DNA
<213> Artificial sequence
<400> 419
catctctaat gcaaacctct ctctctgt 28
<210> 420
<211> 27
<212> DNA
<213> Artificial sequence
<400> 420
ggtggcaaaa atgtagaatg agagtag 27
<210> 421
<211> 25
<212> DNA
<213> Artificial sequence
<400> 421
aacagcagta gcttaggcag attca 25
<210> 422
<211> 27
<212> DNA
<213> Artificial sequence
<400> 422
gcctgtcatc ttctcctcaa actactc 27
<210> 423
<211> 21
<212> DNA
<213> Artificial sequence
<400> 423
gggcaatctt gttcagcatc a 21
<210> 424
<211> 25
<212> DNA
<213> Artificial sequence
<400> 424
ggaattcaaa gctcagacaa ctggt 25
<210> 425
<211> 28
<212> DNA
<213> Artificial sequence
<400> 425
ggacacttga aactctgaaa agttatga 28
<210> 426
<211> 27
<212> DNA
<213> Artificial sequence
<400> 426
gctccgtaaa gagtcttgtg aaaagtc 27
<210> 427
<211> 24
<212> DNA
<213> Artificial sequence
<400> 427
aacacaaccc agactttatg caga 24
<210> 428
<211> 30
<212> DNA
<213> Artificial sequence
<400> 428
aagagagaat atggtgtagt ggttaagcat 30
<210> 429
<211> 26
<212> DNA
<213> Artificial sequence
<400> 429
gcctctgact caactctcct agtgac 26
<210> 430
<211> 21
<212> DNA
<213> Artificial sequence
<400> 430
gccaaattca atgagcacat g 21
<210> 431
<211> 26
<212> DNA
<213> Artificial sequence
<400> 431
gtcaatcata tgtctcttct catggc 26
<210> 432
<211> 29
<212> DNA
<213> Artificial sequence
<400> 432
caaagagata tctgcatcac catattcac 29
<210> 433
<211> 22
<212> DNA
<213> Artificial sequence
<400> 433
ggtagatgac atcctcacct tg 22
<210> 434
<211> 20
<212> DNA
<213> Artificial sequence
<400> 434
<210> 435
<211> 27
<212> DNA
<213> Artificial sequence
<400> 435
gtcagaggct gagactctta gatgatc 27
<210> 436
<211> 28
<212> DNA
<213> Artificial sequence
<400> 436
gagtgtgtgt tttctgctga tagacact 28
<210> 437
<211> 27
<212> DNA
<213> Artificial sequence
<400> 437
gaggcttgtc actaattttt gttgagt 27
<210> 438
<211> 28
<212> DNA
<213> Artificial sequence
<400> 438
tcccaatata agaattagga cctgtgtg 28
<210> 439
<211> 22
<212> DNA
<213> Artificial sequence
<400> 439
ccgggaggct aaggtgaatt ac 22
<210> 440
<211> 26
<212> DNA
<213> Artificial sequence
<400> 440
cactgtatta tgaacagggt tgcaaa 26
<210> 441
<211> 28
<212> DNA
<213> Artificial sequence
<400> 441
gctctgtgac ataccaatca tttctaca 28
<210> 442
<211> 28
<212> DNA
<213> Artificial sequence
<400> 442
ttagccttga ttgactgtaa tccatgta 28
<210> 443
<211> 27
<212> DNA
<213> Artificial sequence
<400> 443
tcatgtctct gttaagacca gaatgct 27
<210> 444
<211> 24
<212> DNA
<213> Artificial sequence
<400> 444
aatgccttcc tcgtttgaac tcta 24
<210> 445
<211> 25
<212> DNA
<213> Artificial sequence
<400> 445
ggtcaaaaga aggcgtagat accct 25
<210> 446
<211> 25
<212> DNA
<213> Artificial sequence
<400> 446
actgctattc ttgtttctcc aggct 25
<210> 447
<211> 28
<212> DNA
<213> Artificial sequence
<400> 447
gcaagtagat gaaaatagca tcttccct 28
<210> 448
<211> 26
<212> DNA
<213> Artificial sequence
<400> 448
agcaaaactt ttcctataga agcaaa 26
<210> 449
<211> 22
<212> DNA
<213> Artificial sequence
<400> 449
atgagtgttg gtgtgagcct ct 22
<210> 450
<211> 22
<212> DNA
<213> Artificial sequence
<400> 450
tcagagcact ggggagaaga at 22
<210> 451
<211> 23
<212> DNA
<213> Artificial sequence
<400> 451
aaaggcagat aagagagatg cct 23
<210> 452
<211> 20
<212> DNA
<213> Artificial sequence
<400> 452
<210> 453
<211> 22
<212> DNA
<213> Artificial sequence
<400> 453
agcaagggag aagaatgtct gg 22
<210> 454
<211> 22
<212> DNA
<213> Artificial sequence
<400> 454
gcccataaac acagcaagat gg 22
<210> 455
<211> 22
<212> DNA
<213> Artificial sequence
<400> 455
ctatgagggc atgggctaac aa 22
<210> 456
<211> 22
<212> DNA
<213> Artificial sequence
<400> 456
ccttcattcc cccacattct ca 22
<210> 457
<211> 22
<212> DNA
<213> Artificial sequence
<400> 457
gctcaatctg caccagtttt gt 22
<210> 458
<211> 22
<212> DNA
<213> Artificial sequence
<400> 458
gccattggtg tctgtaggtc tt 22
<210> 459
<211> 22
<212> DNA
<213> Artificial sequence
<400> 459
ctgcaccagt tttgtgagta gc 22
<210> 460
<211> 22
<212> DNA
<213> Artificial sequence
<400> 460
atctaactgt ggccattggt gt 22
<210> 461
<211> 20
<212> DNA
<213> Artificial sequence
<400> 461
<210> 462
<211> 20
<212> DNA
<213> Artificial sequence
<400> 462
<210> 463
<211> 20
<212> DNA
<213> Artificial sequence
<400> 463
<210> 464
<211> 20
<212> DNA
<213> Artificial sequence
<400> 464
<210> 465
<211> 22
<212> DNA
<213> Artificial sequence
<400> 465
tctaagtaga gaacgagccg gt 22
<210> 466
<211> 22
<212> DNA
<213> Artificial sequence
<400> 466
ggcttcatgg gatacaggga aa 22
<210> 467
<211> 22
<212> DNA
<213> Artificial sequence
<400> 467
agtgaggaaa acacggagtt ga 22
<210> 468
<211> 22
<212> DNA
<213> Artificial sequence
<400> 468
tgtacctcaa cagcctccaa tc 22
<210> 469
<211> 22
<212> DNA
<213> Artificial sequence
<400> 469
caacattttt catgtggccc ct 22
<210> 470
<211> 22
<212> DNA
<213> Artificial sequence
<400> 470
cctctttgat gtccccttcg at 22
<210> 471
<211> 20
<212> DNA
<213> Artificial sequence
<400> 471
<210> 472
<211> 23
<212> DNA
<213> Artificial sequence
<400> 472
ccttacaaat tgtctggaag cat 23
<210> 473
<211> 21
<212> DNA
<213> Artificial sequence
<400> 473
gctgagataa tcgacaagcc a 21
<210> 474
<211> 23
<212> DNA
<213> Artificial sequence
<400> 474
tgcttttgga ttcttggtca tga 23
<210> 475
<211> 22
<212> DNA
<213> Artificial sequence
<400> 475
gcttctcagg tcttctggga aa 22
<210> 476
<211> 22
<212> DNA
<213> Artificial sequence
<400> 476
atatggctaa acctggcacc aa 22
<210> 477
<211> 20
<212> DNA
<213> Artificial sequence
<400> 477
<210> 478
<211> 20
<212> DNA
<213> Artificial sequence
<400> 478
<210> 479
<211> 20
<212> DNA
<213> Artificial sequence
<400> 479
<210> 480
<211> 20
<212> DNA
<213> Artificial sequence
<400> 480
<210> 481
<211> 22
<212> DNA
<213> Artificial sequence
<400> 481
gcaacaaagg ccaaaataga ga 22
<210> 482
<211> 20
<212> DNA
<213> Artificial sequence
<400> 482
<210> 483
<211> 21
<212> DNA
<213> Artificial sequence
<400> 483
tctgtggtct aacaaatgcc c 21
<210> 484
<211> 22
<212> DNA
<213> Artificial sequence
<400> 484
tcatgccaaa gatcttcaca ct 22
<210> 485
<211> 22
<212> DNA
<213> Artificial sequence
<400> 485
tgccaggtaa actgtaaagc aa 22
<210> 486
<211> 22
<212> DNA
<213> Artificial sequence
<400> 486
cctcaaaagg aaagggggaa ga 22
<210> 487
<211> 22
<212> DNA
<213> Artificial sequence
<400> 487
tgtctagctt caaagccaag gt 22
<210> 488
<211> 22
<212> DNA
<213> Artificial sequence
<400> 488
tgagagctga ccaggtagat ga 22
<210> 489
<211> 20
<212> DNA
<213> Artificial sequence
<400> 489
<210> 490
<211> 20
<212> DNA
<213> Artificial sequence
<400> 490
<210> 491
<211> 20
<212> DNA
<213> Artificial sequence
<400> 491
<210> 492
<211> 20
<212> DNA
<213> Artificial sequence
<400> 492
<210> 493
<211> 22
<212> DNA
<213> Artificial sequence
<400> 493
gtatttttga gcagtggctc cg 22
<210> 494
<211> 24
<212> DNA
<213> Artificial sequence
<400> 494
acactagatt cagcaattcc tctg 24
<210> 495
<211> 22
<212> DNA
<213> Artificial sequence
<400> 495
actcctactc ttcctcagtc cc 22
<210> 496
<211> 22
<212> DNA
<213> Artificial sequence
<400> 496
aggtgaactg aggccaaatc at 22
<210> 497
<211> 20
<212> DNA
<213> Artificial sequence
<400> 497
<210> 498
<211> 20
<212> DNA
<213> Artificial sequence
<400> 498
ccaccgcaac aagaagagtc 20
<210> 499
<211> 22
<212> DNA
<213> Artificial sequence
<400> 499
gtgtgaagtt gtgtggcaga ag 22
<210> 500
<211> 22
<212> DNA
<213> Artificial sequence
<400> 500
ctcacacacc tgaaaccaaa gc 22
<210> 501
<211> 22
<212> DNA
<213> Artificial sequence
<400> 501
tgacaccaca tgctgtatgg aa 22
<210> 502
<211> 22
<212> DNA
<213> Artificial sequence
<400> 502
gaatagcgcc caacataggg ta 22
<210> 503
<211> 22
<212> DNA
<213> Artificial sequence
<400> 503
tgggtcactt tatgctgtcc tc 22
<210> 504
<211> 22
<212> DNA
<213> Artificial sequence
<400> 504
ctctttggac ccatcacctc tc 22
<210> 505
<211> 25
<212> DNA
<213> Artificial sequence
<400> 505
ctttaggtca gtatattttg gggtc 25
<210> 506
<211> 22
<212> DNA
<213> Artificial sequence
<400> 506
cttagccctg ggtcttgatg tt 22
<210> 507
<211> 22
<212> DNA
<213> Artificial sequence
<400> 507
atgtgaagct gtagctttga gt 22
<210> 508
<211> 22
<212> DNA
<213> Artificial sequence
<400> 508
taattcagga gctgaactgc cc 22
<210> 509
<211> 22
<212> DNA
<213> Artificial sequence
<400> 509
aatgccaggg acaaacgaat tg 22
<210> 510
<211> 22
<212> DNA
<213> Artificial sequence
<400> 510
tgcagagtaa atgagctgtg gt 22
<210> 511
<211> 20
<212> DNA
<213> Artificial sequence
<400> 511
<210> 512
<211> 20
<212> DNA
<213> Artificial sequence
<400> 512
<210> 513
<211> 22
<212> DNA
<213> Artificial sequence
<400> 513
agaggtgctt tgcgtacctt at 22
<210> 514
<211> 22
<212> DNA
<213> Artificial sequence
<400> 514
tgaaaggctg cctctgttct ac 22
<210> 515
<211> 20
<212> DNA
<213> Artificial sequence
<400> 515
<210> 516
<211> 20
<212> DNA
<213> Artificial sequence
<400> 516
<210> 517
<211> 20
<212> DNA
<213> Artificial sequence
<400> 517
<210> 518
<211> 20
<212> DNA
<213> Artificial sequence
<400> 518
<210> 519
<211> 22
<212> DNA
<213> Artificial sequence
<400> 519
agctgtccaa caaaggacaa ga 22
<210> 520
<211> 22
<212> DNA
<213> Artificial sequence
<400> 520
cctgatgatg atagcgtgca ga 22
<210> 521
<211> 22
<212> DNA
<213> Artificial sequence
<400> 521
ggaagagaca gctccatgtc aa 22
<210> 522
<211> 22
<212> DNA
<213> Artificial sequence
<400> 522
tggggaccag tcttttctga ag 22
<210> 523
<211> 22
<212> DNA
<213> Artificial sequence
<400> 523
accaaaaagt cacatgcagc aa 22
<210> 524
<211> 22
<212> DNA
<213> Artificial sequence
<400> 524
ctgtagctcc attggtgtcc tc 22
<210> 525
<211> 19
<212> DNA
<213> Artificial sequence
<400> 525
atgttgagtg ggaggtgga 19
<210> 526
<211> 20
<212> DNA
<213> Artificial sequence
<400> 526
<210> 527
<211> 20
<212> DNA
<213> Artificial sequence
<400> 527
tggaccgcta catctccatc 20
<210> 528
<211> 20
<212> DNA
<213> Artificial sequence
<400> 528
<210> 529
<211> 20
<212> DNA
<213> Artificial sequence
<400> 529
tggaccgcta catctccatc 20
<210> 530
<211> 20
<212> DNA
<213> Artificial sequence
<400> 530
<210> 531
<211> 21
<212> DNA
<213> Artificial sequence
<400> 531
cccatgtact gcttcatctg c 21
<210> 532
<211> 20
<212> DNA
<213> Artificial sequence
<400> 532
<210> 533
<211> 20
<212> DNA
<213> Artificial sequence
<400> 533
tggaccgcta catctccatc 20
<210> 534
<211> 20
<212> DNA
<213> Artificial sequence
<400> 534
<210> 535
<211> 20
<212> DNA
<213> Artificial sequence
<400> 535
<210> 536
<211> 20
<212> DNA
<213> Artificial sequence
<400> 536
<210> 537
<211> 20
<212> DNA
<213> Artificial sequence
<400> 537
<210> 538
<211> 20
<212> DNA
<213> Artificial sequence
<400> 538
<210> 539
<211> 20
<212> DNA
<213> Artificial sequence
<400> 539
<210> 540
<211> 20
<212> DNA
<213> Artificial sequence
<400> 540
<210> 541
<211> 20
<212> DNA
<213> Artificial sequence
<400> 541
<210> 542
<211> 20
<212> DNA
<213> Artificial sequence
<400> 542
<210> 543
<211> 20
<212> DNA
<213> Artificial sequence
<400> 543
<210> 544
<211> 20
<212> DNA
<213> Artificial sequence
<400> 544
<210> 545
<211> 20
<212> DNA
<213> Artificial sequence
<400> 545
<210> 546
<211> 20
<212> DNA
<213> Artificial sequence
<400> 546
<210> 547
<211> 20
<212> DNA
<213> Artificial sequence
<400> 547
<210> 548
<211> 18
<212> DNA
<213> Artificial sequence
<400> 548
accagcaggt ccgacaag 18
<210> 549
<211> 20
<212> DNA
<213> Artificial sequence
<400> 549
<210> 550
<211> 20
<212> DNA
<213> Artificial sequence
<400> 550
<210> 551
<211> 20
<212> DNA
<213> Artificial sequence
<400> 551
<210> 552
<211> 20
<212> DNA
<213> Artificial sequence
<400> 552
<210> 553
<211> 21
<212> DNA
<213> Artificial sequence
<400> 553
tcctacaaaa ctgagggtcg c 21
<210> 554
<211> 24
<212> DNA
<213> Artificial sequence
<400> 554
cagaacctgt atttcataga ccgt 24
<210> 555
<211> 20
<212> DNA
<213> Artificial sequence
<400> 555
<210> 556
<211> 20
<212> DNA
<213> Artificial sequence
<400> 556
<210> 557
<211> 20
<212> DNA
<213> Artificial sequence
<400> 557
<210> 558
<211> 20
<212> DNA
<213> Artificial sequence
<400> 558
<210> 559
<211> 18
<212> DNA
<213> Artificial sequence
<400> 559
cggtgagcag agatcgtg 18
<210> 560
<211> 20
<212> DNA
<213> Artificial sequence
<400> 560
<210> 561
<211> 22
<212> DNA
<213> Artificial sequence
<400> 561
ccatgccgat tcttcatgtt gt 22
<210> 562
<211> 25
<212> DNA
<213> Artificial sequence
<400> 562
gtgtttccag gcatttatcc atttc 25
Claims (9)
1. The primer combination is characterized by comprising 562 primer fragments for simultaneously amplifying 224 InDel sites and 57 SNP sites, wherein RS numbers of the InDel sites are respectively as follows: rs 1407956, rs3067397, rs10607699, rs142717783, rs3054057, rs17878444, rs71852971, rs 1418539739, rs138331044, rs59841142, rs76941525, rs2307924, rs10572185, rs67487831, rs11277697, rs 355240, rs113011930, rs 383636186, rs28369942, rs35484769, rs 340006, rs2307959, rs3038275, rs146875868, rs2067235, rs 455836, rs 145145158, rs 106588 588, rs 66477476, rs35136650, rs 90825, rs147792696, rs 383434799, rs 3834799, rs 3556356735563567356735793, rs 35563556355635794, rs 4354776735563556355635679, rs 35729, rs 3556355635563556355635563556355635729, rs 3556355635563556355635729, rs 3556355635563556355635563556355635563556355635567, rs 355635563556355635563556355648, rs 355635567, rs 3556355635563556355635567, rs 3556355635563556355635563556355648, rs 355635563556355635567, rs 355635563556355635563556355635567, rs 35563556355635563556355648, rs 355635567, rs 3556355648, rs 355648, rs 3556355648, rs 3556355635563556355648, rs 355648, rs 35563556355648, rs 355648, rs 3556355648, rs 35563556355635563556355648, rs 35563556355635563556355635567, rs 355635567, rs 3556355648, rs 35563556355648, rs 355648, rs 3556355648, rs 35563556355635563556355648, rs 3556355648, rs 355648, rs 3556355648, rs 35563556355635563556355648, rs 35563556355648, rs 355635563556355635563556355635567, rs 3556355648, rs 355648, rs 3556355648, rs 355648, rs 3556355635563556355648, rs 355635567, rs 3556355635563556355648, rs 355648, rs 3556355635567, rs 355648, rs 355635567, rs 35567, rs 355648, rs 35567, rs 355648, rs 3556355648, rs 355648, rs 35563556355648, rs 355648, rs 35567, rs 355648, rs 35567, rs 3556355648, rs 355648, rs 3556355635563556355635563556355635563556355648, rs 35567, rs 355648, rs 3556355635563556355648, rs 355648, rs 355635563556355648, rs 35567, rs No, rs 355648, rs 3556, rs571755931, rs139762874, rs11444232, rs34419736, rs 7763535204, rs34421865, rs58269429, rs66739142, rs2307433, rs10626599, rs35193388, rs 10610210201, rs1610905, rs11282585, rs10641793, rs 145010051041, rs 306949460, rs 7581, rs1305047, rs 167830330333, rs 91333380, rs530345654, rs 72009, rs 38383818, rs 339673, rs77206391, rs5825653, rs 339783, rs 5436599, rs 72555595, rs34529638, rs5828358 358, rs 71448, rs16438, rs 00059476, rs35455, rs 5961616161577956795679567980, rs 3579577981798, rs 35798179817948, rs 35798, rs 35563579817948, rs 357981798, rs 3556355635798, rs 35567777777777777777798, rs 35563477777777777777567, rs 35798, rs 355634777777777777777781798, rs 35567, rs 35563477567, rs 355634798, rs 3556347356375637563756375637567, rs 3556375637567, rs 355634775634798, rs 3556375637567, rs 355637567, rs 3556347756375637567, rs 355637563756375637563756375637567, rs 355637567, rs 355637563756375637567, rs 355637563756375637563756375637567, rs 3556375637563756375637567, rs 355637567, rs 35567, rs 35563756375637563756375637563756375637567, rs 355637567, rs 355637563756375637567, rs 35563756375637563756375637567, rs 35567, rs 3556375637563756375637567, rs 355634798, rs 35563756375637567, rs 355637567, rs 35567, rs 355634798, rs 35567, rs 355637563756375637563756375637567, rs 355637563756375637563756375637563756375637563756375637563756375637567, rs 35567, rs 355637563756375637567, rs 3556375637563756375637567, rs 35567, rs 355637567, rs 3556375637563756375637563756375637563756375637563756375637563756375637563756375637567, rs 3556375637567, rs 35567, rs 355637567, rs 3556375637563756375637567, rs 35563756375637563756375637567, rs 35567, rs 355637567, rs 35567, rs 3556375637567, rs 35567, rs 35563756375637567, rs 35567, rs 355637563756375637567, rs 355637567, rs 3556375637563756375637563756375637563756375637563756375637563756375637563756375637567, rs 3556375637567, rs 35567, rs 355637567, rs 35563756375637; RS numbers of the SNP loci are respectively as follows: rs7516137, rs6669519, rs10923710, rs4915551, rs3827760, rs12473319, rs12632544, rs12633011, rs2045323, rs16891982, rs28777, rs1470608, rs147468294, rs12203592, rs4959270, rs111847181, rs11988731, rs683, rs10756819, rs4749259, rs12258832, rs3740550, rs1126809, rs1393350, rs1042602, rs12821256, rs 2402132130, rs17128291, rs 96399, rs1426654, rs1800414, rs1667394, 1801801801801801801807, rs2238289, rs12913832, rs 414197292, rs 5397, rs 1805005005007, rs1805008, rs 32464, rs 324747849, rs 18051675167435445, 5005632849, rs 18050050050050050050050005, and 50050050050050050055.
2. The primer combination of claim 1, wherein the nucleotide sequences of 562 primer fragments are shown in SEQ ID No. 1-562 respectively.
3. Use of a primer combination according to claim 1 or 2 for individual identification, genetic relationship identification, progenitor inference and phenotype inference.
4. A kit comprising the primer combination of claim 1 or 2.
5. The kit of claim 4, further comprising a PCR buffer and/or a DNA polymerase.
6. The kit of claim 5, further comprising a DNA standard comprising 9948 genomic DNA and/or 9947A genomic DNA.
7. The kit according to any one of claims 4 to 6, further comprising at least one of a DNA extraction reagent, a PCR amplification reagent, a sequencing library construction reagent, and a nucleic acid purification reagent.
8. Use of a kit according to any one of claims 4 to 7 for individual identification, genetic relationship identification, ancestral inference and phenotypic inference.
9. A DNA typing method, characterized in that a sample to be tested is subjected to PCR amplification by using the primer combination of any one of claims 1 to 2 or the kit of any one of claims 4 to 7, and the amplified product is sequenced by a second generation DNA sequencing technology to obtain the DNA typing of the sample to be tested.
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