CN114774250A - Microorganism regular sampling detection device - Google Patents

Microorganism regular sampling detection device Download PDF

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Publication number
CN114774250A
CN114774250A CN202210484830.0A CN202210484830A CN114774250A CN 114774250 A CN114774250 A CN 114774250A CN 202210484830 A CN202210484830 A CN 202210484830A CN 114774250 A CN114774250 A CN 114774250A
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sampling
culture dish
wall
pipe
incubator
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CN202210484830.0A
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Inventor
张慧
马连杰
卢文才
廖敦秀
唐荣莉
余端
李燕
胡留杰
梁涛
杭晓宁
张健
王琳
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Chongqing Academy of Agricultural Sciences
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Chongqing Academy of Agricultural Sciences
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Priority to CN202210484830.0A priority Critical patent/CN114774250A/en
Publication of CN114774250A publication Critical patent/CN114774250A/en
Withdrawn legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/10Petri dish
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/06Tubular
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/50Means for positioning or orientating the apparatus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
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  • Thermal Sciences (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention relates to the technical field of microorganism sampling, in particular to a microorganism periodic sampling detection device, which comprises an incubator, wherein a sampling box is arranged on one side of the incubator, an incubator fixing cover and an upper pressing plate are arranged above the incubator and the sampling box, a sampling tube mounting assembly is arranged on the outer wall of the sampling box, a culture dish storage assembly is arranged inside the incubator and comprises a central column, eight movable plate fixing frames are arranged on the outer wall of the central column, a sampling tube mounting assembly is arranged inside the device, the position of a first electric cylinder assembly can be controlled through the sampling tube mounting assembly, different automatic sampling assemblies can be grabbed through the first electric cylinder assembly, substances in a culture dish on a vessel limiting plate can be sampled through the automatic sampling assemblies, and the device is positioned inside the sampling box, can be isolated from the outside, and reduce the pollution when sampling.

Description

Microorganism regular sampling detection device
Technical Field
The invention relates to the technical field of microorganism sampling, in particular to a microorganism regular sampling detection device.
Background
The microorganisms comprise bacteria, viruses, fungi, a large group of small protists, microalgae and the like, the individual is tiny, is closely related to human beings, covers various beneficial and harmful types, and widely relates to various fields such as food, medicine, industry and agriculture, environmental protection, sports and the like.
Chinese utility model patent (CN 215209424U) discloses a microorganism periodic sampling detection device, which comprises a box body, a disinfection cleaning device is arranged in the box body, the disinfection cleaning device comprises a disinfection cylinder, the lower surface of the inner wall of the disinfection cylinder is provided with a first motor, the output end of the first motor is provided with an output shaft, the upper end of the output shaft is provided with a cleaning cylinder, the lower surface of the outer wall of the cleaning cylinder is provided with a plurality of circulation holes, the inner wall of the cleaning cylinder is provided with a cleaning soft hair layer, one side of the inner wall of the disinfection cylinder is provided with a water-stop sheet, the middle of the water-stop sheet is provided with a round hole, a waterproof sealing ring is arranged in the round hole, the inner wall of the waterproof sealing ring is rotationally connected with the output shaft, the top of the inner wall of the box body is provided with a sampling device, through the disinfection cleaning device, the sampling tube needle of a sampling extractor is disinfected and cleaned after each sampling, the influence on the next sampling is avoided, the accuracy of the detection is improved, and the structure is simple, the operation is easy, and certain practical value is achieved.
The following problems are involved:
(1) the disinfection tube is arranged in the disinfection tube for disinfection in the use process of the existing equipment, when the disinfection tube is used, the disinfection tube has no sealing effect, and the disinfection liquid in the disinfection tube can be exposed in the air, so that the volatilization of active ingredients in the disinfection liquid is caused, the disinfection liquid is inactivated, and the cross contamination of culture substances in the disinfection tube is caused.
(2) Current equipment still relies on external incubator to cultivate at the in-process that uses, at the in-process that shifts the culture dish like this, leads to the inside condition that pollutes that appears of culture dish easily, leads to the condition that follow-up detection error appears, reduces the effect of experiment.
Accordingly, there is a need to devise a solution that addresses one or more of the above-identified problems.
Disclosure of Invention
In order to solve one or more problems in the prior art, the invention provides a microorganism periodic sampling detection device.
The technical scheme adopted by the invention to achieve the aim is as follows: a microorganism periodic sampling detection device comprises an incubator, wherein a sampling box is arranged on one side of the incubator, an incubator fixing cover and an upper pressing plate are arranged above the incubator and the sampling box, a sampling tube mounting assembly is arranged on the outer wall of the sampling box, and a culture dish storage assembly is arranged inside the incubator; the culture dish storage assembly comprises a central column, eight movable plate fixing frames are arranged on the outer wall of the central column, cylindrical movable pipes are fixedly arranged among the eight movable plate fixing frames, the upper part of the moving tube is open, the inner side of the moving tube is provided with a culture dish fixing tube, the bottom of the moving tube is provided with a dish sliding seat, and the outer wall of the movable pipe is provided with a limit pipe and a third electric cylinder component, and a linkage block is arranged in the limit pipe in a sliding way, one side of the linkage block is fixedly connected with the outer wall of the culture dish fixing tube, the output end of the third electric cylinder component is connected with an arc-shaped pushing frame, the pushing frame is arranged in the moving pipe, the outer wall of the moving pipe is provided with a through hole corresponding to the position of the pushing frame, the sampling box is connected the terminal surface with the incubator and is seted up and to push away the frame outside and push away the corresponding through-hole in frame position, the below of removing the pipe is provided with lever assembly.
Preferably, the sampling tube installation component comprises a first side box and a second side box, wherein the first side box and the second side box are symmetrically distributed about the central line of the sampling box, the first side box and the second side box are communicated with the inside of the sampling box, the outer walls of the first side box and the second side box are provided with through holes, side doors are arranged in the through holes of the outer walls of the first side box and the second side box, and the placing frames are arranged inside the first side box and the second side box.
Preferably, the rack includes two slides and slide, two the slide symmetry form sets up in first side case and second side incasement, and the slide slides and sets up two between the slide, the through-hole has been seted up to the outer wall of slide, and the interpolation of slide outer wall through-hole is equipped with automatic sampling subassembly.
Preferably, sampling box, first side case and the inside biax lead screw transmission slip table that is provided with of second side case, the output of biax lead screw transmission slip table is connected with first electric cylinder subassembly, and the output of first electric cylinder subassembly is provided with electronic clamping jaw, the inner wall of sampling box is provided with the second electric cylinder subassembly, and the output of second electric cylinder subassembly is connected with pneumatic suction nozzle, the inside of sampling box is provided with the household utensils limiting plate, and the sideslip of household utensils limiting plate is provided with the movable plate, and the outer wall of sampling box sets up the through-hole identical with movable plate external shape, household utensils limiting plate top height highly maintains an equal level with the top of household utensils sliding seat, the top of movable plate is provided with the kicking block, and sets up on the household utensils limiting plate with kicking block external shape assorted recess.
Preferably, the upper end surface of the vessel sliding seat is provided with a through hole, a ball is arranged in the through hole in the upper end of the vessel sliding seat, a cavity is formed in the vessel sliding seat, a transmission belt is arranged in the cavity of the vessel sliding seat, the lower end of the ball is attached to the upper end surface of the transmission belt, and a driving motor is arranged on the inner side of the transmission belt.
Preferably, the inner wall of the culture dish fixing pipe is provided with a cavity, a resistance heating plate is arranged in the cavity of the culture dish fixing pipe, the lower end of the resistance heating plate is provided with a sliding ring, the bottom of the incubator is provided with an annular groove matched with the external shape of the sliding ring, and an electric connection assembly is arranged in the annular groove at the bottom of the incubator.
Preferably, the electric connection assembly comprises a metal ball, a conductive spring is arranged below the metal ball, and a connection line is arranged on the inner side of the conductive spring.
Preferably, the third electric cylinder component comprises a power motor, a transmission gear and a lead screw main body, the power motor is in transmission connection with the lead screw main body through a gear, one end of the lead screw main body is fixedly connected with the pushing frame, the other end of the lead screw main body is rotatably connected with a linkage rod, the other end of the linkage rod is hinged to the outer wall of the linkage block, and a strip-shaped through hole matched with the shape of the linkage rod is formed in the outer wall of the limiting pipe.
Preferably, the outer wall of the incubator fixing cover is provided with a through hole, a culture dish feeding pipe is arranged outside the through hole at the top of the incubator fixing cover, and the position of the culture dish feeding pipe corresponds to that of the moving pipe.
Preferably, the lever subassembly includes two rotation levers and two lever guide holders, two the articulated lower extreme that sets up at the fixed pipe of culture dish in the center of rotating the lever, two the outside parcel of rotating the lever is provided with the barrier film, two the both ends of rotating the lever are provided with the columniform arch, and two lever guide holders are the slope form and set up in the bottom of removing the pipe, and the outer wall of lever guide holder seted up with rotate the protruding external shape assorted spout of lever one end.
The beneficial effects of the invention are:
(1) the culture dish fixing pipes and the moving pipes are arranged in the culture dish storage device, the culture dishes of the same type can be stored and cultured through the culture dish fixing pipes and the moving pipes, meanwhile, the resistance heating plates are arranged in the moving pipes, so that the temperature in the moving pipes of different groups can be changed, and different types of fungi can be cultured conveniently;
(2) the sampling tube mounting assembly is arranged in the sampling box, the position of the first electric cylinder assembly can be controlled through the sampling tube mounting assembly, different automatic sampling assemblies can be grabbed through the first electric cylinder assembly, substances in a culture dish on a utensil limiting plate are sampled through the automatic sampling assemblies, and meanwhile, the device is positioned in the sampling box and can be isolated from the outside, so that the pollution during sampling is reduced, the use of disinfectant is reduced, and the pollution of sampled culture substances is avoided;
(3) the bottom of the moving pipe is provided with the slip ring, the slip ring can be clamped in the groove at the bottom of the inner side of the incubator and can limit the moving path of the moving pipe, the stability of the moving pipe in rotation is improved, meanwhile, the slip ring can be connected with the metal ball, electric power is led into the resistance heating plate through the slip ring, the metal ball, the conductive spring and the connecting wire, and the electric power is provided for the resistance heating plate
(4) The upper end surface of the vessel sliding seat is provided with the balls, the moving effect of the culture vessel is improved through the balls, and meanwhile, the bottoms of the balls are transmitted through the transmission belt, so that the balls can perform the recovery work on the culture vessel, and the culture vessel can enter other moving pipes again;
(5) according to the invention, the lever assembly is arranged at the bottom of the culture dish fixing tube, the isolating membrane is arranged outside the rotating lever in the lever assembly, so that the condition that the culture dish is scratched by the isolating membrane is prevented, and meanwhile, the rotating lever can be matched with the lever guide seat to perform tilting work, so that the lever guide seat can perform limiting work on the culture dish in the culture dish fixing tube, and the condition that the culture dish in the culture dish fixing tube slides downwards is prevented.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a top view of the present invention;
FIG. 3 is a schematic view of a moving tube structure according to the present invention;
FIG. 4 is a cross-sectional view taken at A-A of FIG. 2 in accordance with the present invention;
FIG. 5 is a cross-sectional view taken at B-B of FIG. 2 in accordance with the present invention;
FIG. 6 is a cross-sectional view taken at C-C of FIG. 4 in accordance with the present invention;
FIG. 7 is an enlarged view of the structure of FIG. 4;
FIG. 8 is an enlarged view of E of FIG. 4 according to the present invention;
FIG. 9 is an enlarged view of the structure of FIG. 6 at F according to the present invention;
FIG. 10 is a schematic view of the structure of the petri dish fixing tube of the invention.
Reference numerals: 1. a incubator fixing cover, 2, an incubator, 3, an upper pressure plate, 4, a sampling box, 5, a moving plate, 6, a first side box, 7, a second side box, 8, a culture dish feeding pipe, 9, a culture dish fixing pipe, 10, a moving plate fixing frame, 11, a moving pipe, 12, a central column, 13, a utensil sliding seat, 14, a limiting pipe, 15, a side door, 16, a linkage rod, 17, a double-shaft screw rod transmission sliding table, 18, a first electric cylinder component, 19, an automatic sampling component, 20, a second electric cylinder component, 21, a utensil limiting plate, 22, a servo motor, 23, a third electric cylinder component, 24, a placing frame, 25, a grabbing component, 26, a linkage block, 27, a pushing frame, 28, a resistance heating plate, 29, a sliding ring, 30, a metal ball, 31, a conductive spring, 32, a connecting line, 33, an isolating membrane, 34, a rotating lever, 35, a lever guide seat, 36 and a ball, 37. and (4) a transmission belt.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in detail below. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.
As shown in fig. 1-10, the invention provides a microorganism periodic sampling detection device, which comprises an incubator 2, wherein a sampling box 4 is arranged on one side of the incubator 2, an incubator fixing cover 1 and an upper pressing plate 3 are arranged above the incubator 2 and the sampling box 4, a sampling tube mounting assembly is arranged on the outer wall of the sampling box 4, and a culture dish storage assembly is arranged inside the incubator 2; the culture dish storage assembly comprises a central column 12, eight movable plate fixing frames 10 are arranged on the outer wall of the central column 12, cylindrical movable tubes 11 are fixedly arranged among the eight movable plate fixing frames 10, the upper parts of the movable tubes 11 are in an opening shape, a culture dish fixing tube 9 is arranged on the inner side of each movable tube 11, a utensil sliding seat 13 is arranged at the bottom of each movable tube 11, a limiting tube 14 and a third electric cylinder assembly 23 are arranged on the outer wall of each movable tube 11, a linkage block 26 is slidably arranged in each limiting tube 14, one side of each linkage block 26 is fixedly connected with the outer wall of each culture dish fixing tube 9, the output end of each third electric cylinder assembly 23 is connected with an arc-shaped push frame 27, each push frame 27 is arranged in each movable tube 11, through holes corresponding to the positions of the push frames 27 are formed in the outer wall of each movable tube 11, through holes corresponding to the positions of the push frames 27 are formed in the connecting end faces of the sampling box 4 and the culture box 2, a lever assembly is arranged below the moving pipe 11.
The incubator fixing cover 1 and the upper pressure plate 3 can be detached, so that a user can conveniently maintain the equipment inside the incubator 2 and the sampling box 4, the bottom of the central column 12 is provided with the servo motor 22, the central column 12 can be controlled to rotate through the servo motor 22, the central column 12 can control the moving tube 11 to move, the moving tube 11 is internally provided with the culture dish fixing tube 9, the culture dish can be limited through the culture dish fixing tube 9, the bottom of the culture dish fixing tube 9 is provided with the lever assembly, the lever assembly can limit the position of the culture dish, the user can conveniently control the work, the third electric cylinder assembly 23 can push the push frame 27 to move, the push frame 27 can push the culture dish to move, the culture dish is pushed onto the dish limiting plate 21, and culture works of different types can be carried out through the multiple groups of moving tubes 11, manual transfer is reduced, and the condition that the culture dish is polluted is avoided.
Further, in the above technical solution, the sampling tube mounting assembly includes a first side box 6 and a second side box 7, the first side box 6 and the second side box 7 are symmetrically distributed about the center line of the sampling box 4, and the first side box 6 and the second side box 7 are communicated with the inside of the sampling box 4, the outer walls of the first side box 6 and the second side box 7 are provided with through holes, and the through holes of the outer walls of the first side box 6 and the second side box 7 are both provided with a side door 15, and the insides of the first side box 6 and the second side box 7 are both provided with a placing rack 24; further, in the above technical scheme, the placing rack 24 includes two slides and a slide plate, the two slides are symmetrically arranged in the first side box 6 and the second side box 7, the slide plate is slidably arranged between the two slides, a through hole is formed in the outer wall of the slide plate, and the automatic sampling assembly 19 is inserted into the through hole in the outer wall of the slide plate.
Side door 15 homoenergetic of first side case 6 and second side case 7 one side can be opened, the slide can slide simultaneously, the slide is from the interior roll-off of slide, the convenience is changed automatic sampling subassembly 19 on the slide, first side case 6 and second side case 7 keep apart automatic sampling subassembly 19 with external environment simultaneously, avoid automatic sampling subassembly 19 the contaminated condition to appear, automatic sampling subassembly 19 adopts electric control's sampling tube, automatic sampling subassembly 19 is prior art, so do not do much here and describe.
Further, in the above technical scheme, the sampling box 4, the inside biax lead screw transmission slip table 17 that is provided with of first side case 6 and second side case 7, the output of biax lead screw transmission slip table 17 is connected with first electric cylinder component 18, and the output of first electric cylinder component 18 is provided with electronic clamping jaw, the inner wall of sampling box 4 is provided with second electric cylinder component 20, and the output of second electric cylinder component 20 is connected with pneumatic suction nozzle, the inside of sampling box 4 is provided with household utensils limiting plate 21, and one side of household utensils limiting plate 21 slides and is provided with movable plate 5, and the outer wall of sampling box 4 has seted up the through-hole identical with movable plate 5 external shape, household utensils limiting plate 21 topmost highly maintains an equal level with the top of household utensils sliding seat 13.
Biax lead screw transmission slip table 17 can drive first electric cylinder subassembly 18 and carry out the removal of XY axle, plays the effect of the first electric cylinder subassembly 18 position of control, and the electronic of the output of first electric cylinder subassembly 18 is cliied and can be had automatic sampling subassembly 19, can make automatic sampling subassembly 19 remove simultaneously, makes automatic sampling subassembly 19 can remove the work of taking a sample on the culture dish of difference, and household utensils limiting plate 21 and movable plate 5 play the effect of fixed different culture dishes.
Further, in the above technical scheme, a through hole is formed in the upper end surface of the vessel sliding seat 13, a ball 36 is arranged in the through hole in the upper end of the vessel sliding seat 13, a cavity is formed in the vessel sliding seat 13, a transmission belt 37 is arranged in the cavity of the vessel sliding seat 13, the lower end of the ball 36 is attached to the upper end surface of the transmission belt 37, a driving motor is arranged on the inner side of the transmission belt 37, a top block 38 is arranged above the moving plate 5, and a groove matched with the external shape of the top block 38 is formed in the vessel limiting plate 21.
The transmission belt 37 is arranged inside the dish sliding seat 13, the transmission belt 37 drives the balls 36 to roll, so that the balls 36 can drive the culture dish to slide, and meanwhile, the balls 36 are in a rolling state, so that the flexibility of the culture dish is improved.
Further, in the above technical scheme, a cavity is formed in the inner wall of the culture dish fixing tube 9, a resistance heating plate 28 is arranged in the cavity of the culture dish fixing tube 9, a sliding ring 29 is arranged at the lower end of the resistance heating plate 28, an annular groove matched with the external shape of the sliding ring 29 is formed in the bottom of the incubator 2, and an electric connection assembly is arranged in the annular groove in the bottom of the incubator 2.
The inside resistance heating board 28 that is provided with of culture dish fixed tube 9 heats removal pipe 11 through resistance heating board 28 inside, conveniently removes 11 inside temperature control of pipe like this, and the removal pipe 11 of difference simultaneously can be controlled alone, can carry out different types of bacterial colony like this and cultivate, and resistance heating board 28 passes through electrical connection component and is connected with external power supply, to the effect of resistance heating board 28 power transmission power.
Further, in the above technical solution, the electrical connection assembly includes a metal ball 30, a conductive spring 31 is disposed below the metal ball 30, and a connection line 32 is disposed inside the conductive spring 31.
The conductive spring 31 pushes the metal ball 30 under the slide ring 29 so that the slide ring 29 can transmit power into the resistance heating plate 28 and the slide ring 29 can be clamped in the groove at the bottom of the inner side of the incubator 2, thereby improving the stability of the movement of the moving tube 11.
Further, in the above technical scheme, the third electric cylinder assembly 23 includes a power motor, a transmission gear and a lead screw main body, the power motor is in transmission connection with the lead screw main body through the gear, one end of the lead screw main body is fixedly connected with the pushing frame 27, the other end of the lead screw main body is rotatably connected with the linkage rod 16, the other end of the linkage rod 16 is hinged to the outer wall of the linkage block 26, and the outer wall of the limiting pipe 14 is provided with a strip-shaped through hole matched with the linkage rod 16 in shape.
The third electric cylinder assembly 23 is used for driving the linkage block 26 and the pushing frame 27, the linkage block 26 can be connected with the culture dish fixing tube 9, so that the culture dish fixing tube 9 can be driven to move up and down, the pushing frame 27 can move left and right, and a culture dish is pushed out from the moving tube 11 through the pushing frame 27.
Further, in the above technical scheme, the outer wall of the incubator fixing cover 1 is provided with a through hole, and a culture dish feeding pipe 8 is arranged outside the through hole at the top of the incubator fixing cover 1, and the culture dish feeding pipe 8 corresponds to the position of the moving pipe 11.
Further, in above-mentioned technical scheme, the lever subassembly includes two rotation levers 34 and two lever guide holder 35, and two articulated settings in the lower extreme of the fixed pipe 9 of culture dish of the center of rotating lever 34, and two outside parcels that rotate lever 34 are provided with barrier film 33, and two both ends that rotate lever 34 are provided with columniform arch, and two lever guide holders 35 are the slope form and set up in the bottom that removes pipe 11, and the outer wall of lever guide holder 35 seted up with rotate the protruding outside shape assorted spout in lever 34 one end.
The fixed pipe of culture dish 9 can drive the lever subassembly and remove, and the one end of the rotation lever 34 in the lever subassembly can slide along the spout of lever guide holder 35, and the other end that rotates lever 34 like this can rotate, and the other end that rotates lever 34 can live the position restriction of culture dish, plays the effect of fixed culture dish position.
The working principle is as follows: before the device is used, a culture dish filled with bacterial colonies is arranged in a culture dish fixing tube 9 through a culture dish feeding tube 8, the culture dish slides to the position of a lever assembly, the culture dish is clamped through a rotating lever 34 with one end tilted, then electric power is transmitted to a resistance heating plate 28 through an electric connection assembly, the interior of a moving tube 11 is heated through the resistance heating plate 28, the temperature of the interior of the culture dish fixing tube 9 is controlled, meanwhile, an unused automatic sampling assembly 19 is arranged on a placing frame 24 in a first side box 6, a side door 15 is fixed on the first side box 6 and a second side box 7 again, then the unused culture dish is arranged on a moving plate 5, then the moving plate 5 is pushed into a sampling box 4, and when the moving plate needs to be sampled, a pushing frame 27 is controlled through a third electric cylinder assembly 23 to push the culture dish to enter a position of a vessel limiting plate 21, meanwhile, the ejector block 38 on the movable plate 5 is clamped in the groove on the vessel limiting plate 21, the culture dish on the vessel limiting plate 21 is clamped through the ejector block 38, the culture dish on the vessel limiting plate 21 is prevented from shaking, then the second electric cylinder assembly 20 is started, the cover on the culture dish is opened through the second electric cylinder assembly 20, then the first electric cylinder assembly 18 is controlled to move through the double-shaft screw rod transmission sliding table 17, the unused automatic sampling assembly 19 is fixed through the clamping jaw of the first electric cylinder assembly 18, then the double-shaft screw rod transmission sliding table 17 controls the automatic sampling assembly 19 to sample the culture dish on the vessel limiting plate 21, then the double-shaft screw rod transmission sliding table 17 controls the automatic sampling assembly 19 to move to the culture dish on the movable plate 5, the culture dish on the movable plate 5 is sampled, after sampling is completed, the double-shaft screw rod transmission sliding table 17 controls the automatic sampling assembly 19 to move to the placing rack 24 inside the second side box 7, the used automatic sampling assembly 19 can be independently stored, finally the servo motor 22 drives the moving tube 11 to rotate, the moving tube 11 of the culture dish which is not stored is moved to the position corresponding to the sampling box 4, the sealing cover of the culture dish on the vessel limiting plate 21 is loosened through the air nozzle on the second electric cylinder assembly 20, then the moving plate 5 is pressed, the culture dish is pushed back into the moving tube 11 by the jacking block 38 on the moving plate 5, meanwhile, the ball 36 is driven by the driving belt 37 to roll, the culture dish is driven to enter the lower part of the culture dish fixing tube 9 through the ball 36, meanwhile, the culture dish is clamped by the rotating lever 34 at the lower end of the culture dish fixing tube 9, then the culture dish fixing tube 9 moves upwards under the action of the linkage rod 16, so that a certain distance exists between the culture dish fixing tube 9 and the upper part of the vessel sliding seat 13, and the next culture dish can conveniently enter the upper part of the vessel sliding seat 13, the recovery work of the culture dish is performed.
The above-mentioned embodiments only express one or more embodiments of the present invention, and the description is specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims (10)

1. The utility model provides a microorganism periodic sampling detection device, includes incubator (2), one side of incubator (2) is provided with sampling box (4), the top of incubator (2) and sampling box (4) is provided with incubator fixed cover (1) and top board (3), its characterized in that: a sampling tube mounting assembly is arranged on the outer wall of the sampling box (4), and a culture dish storage assembly is arranged inside the culture box (2); the culture dish storage assembly comprises a central column (12), eight movable plate fixing frames (10) are arranged on the outer wall of the central column (12), a cylindrical moving pipe (11) is fixedly arranged between the eight movable plate fixing frames (10), the upper portion of the moving pipe (11) is in an opening shape, a culture dish fixing pipe (9) is arranged on the inner side of the moving pipe (11), a utensil sliding seat (13) is arranged at the bottom of the moving pipe (11), a limiting pipe (14) and a third electric cylinder assembly (23) are arranged on the outer wall of the moving pipe (11), a linkage block (26) is arranged in the limiting pipe (14) in a sliding mode, one side of the linkage block (26) is fixedly connected with the outer wall of the culture dish fixing pipe (9), an arc-shaped pushing frame (27) is connected to the output end of the third electric cylinder assembly (23), and the pushing frame (27) is arranged in the moving pipe (11), the outer wall of the moving pipe (11) is provided with a through hole corresponding to the position of the pushing frame (27), the connecting end face of the sampling box (4) and the incubator (2) is provided with a through hole corresponding to the position of the pushing frame (27) outside, and a lever assembly is arranged below the moving pipe (11).
2. The periodic sampling and testing device for microorganisms according to claim 1, wherein: sampling tube installation component includes first side case (6) and second side case (7), first side case (6) and second side case (7) are about the central line symmetric distribution of sampling case (4), and first side case (6) and second side case (7) are linked together with sampling case (4) inside, the through-hole has been seted up to the outer wall of first side case (6) and second side case (7), and all is provided with side door (15) in first side case (6) and second side case (7) outer wall through-hole, the inside of first side case (6) and second side case (7) all is provided with rack (24).
3. The periodic microorganism sampling and testing device as set forth in claim 2, wherein: the placing frame (24) comprises two slideways and two sliding plates, wherein the slideways are symmetrically arranged in a first side box (6) and a second side box (7), the sliding plates are arranged between the slideways in a sliding mode, through holes are formed in the outer walls of the sliding plates, and automatic sampling assemblies (19) are inserted into the through holes in the outer walls of the sliding plates.
4. The periodic sampling and testing device for microorganisms according to claim 1, wherein: the utility model discloses a sampling device, including sampling case (4), first side case (6) and second side case (7) inside be provided with biax screw drive slip table (17), the output of biax screw drive slip table (17) is connected with first electric cylinder subassembly (18), and the output of first electric cylinder subassembly (18) is provided with electronic clamping jaw, the inner wall of sampling case (4) is provided with second electric cylinder subassembly (20), and the output of second electric cylinder subassembly (20) is connected with pneumatic suction nozzle, the inside of sampling case (4) is provided with household utensils limiting plate (21), and one side slip of household utensils limiting plate (21) is provided with movable plate (5), and the outer wall of sampling case (4) is seted up the through-hole identical with movable plate (5) external shape, the top height of household utensils limiting plate (21) keeps level with the top height of household utensils sliding seat (13), the top of movable plate (5) is provided with kicking block (38), and the vessel limiting plate (21) is provided with a groove matched with the external shape of the top block (38).
5. The periodic microorganism sampling and testing device as set forth in claim 1, wherein: the through-hole has been seted up to the up end of household utensils sliding seat (13), and is provided with ball (36) in household utensils sliding seat (13) upper end through-hole, the inside cavity of having seted up of household utensils sliding seat (13), and be provided with drive belt (37) in the cavity of household utensils sliding seat (13), the lower extreme laminating of ball (36) is at drive belt (37) up end, the inboard of drive belt (37) is provided with driving motor.
6. The periodic microorganism sampling and testing device as set forth in claim 1, wherein: the inner wall of the culture dish fixing pipe (9) is provided with a cavity, a resistance heating plate (28) is arranged in the cavity of the culture dish fixing pipe (9), the lower end of the resistance heating plate (28) is provided with a sliding ring (29), the bottom of the culture box (2) is provided with an annular groove matched with the external shape of the sliding ring (29), and an electric connection assembly is arranged in the annular groove at the bottom of the culture box (2).
7. The periodic microorganism sampling and testing device as set forth in claim 6, wherein: the electric connection assembly comprises a metal ball (30), a conductive spring (31) is arranged below the metal ball (30), and a connecting wire (32) is arranged on the inner side of the conductive spring (31).
8. The periodic microorganism sampling and testing device as set forth in claim 1, wherein: the third electric cylinder component (23) comprises a power motor, a transmission gear and a lead screw main body, the power motor is in transmission connection with the lead screw main body through a gear, one end of the lead screw main body is fixedly connected with a pushing frame (27), the other end of the lead screw main body is rotatably connected with a linkage rod (16), the other end of the linkage rod (16) is hinged to the outer wall of a linkage block (26), and a strip-shaped through hole matched with the shape of the linkage rod (16) is formed in the outer wall of a limiting pipe (14).
9. The periodic microorganism sampling and testing device as set forth in claim 1, wherein: the outer wall of the incubator fixing cover (1) is provided with a through hole, a culture dish inlet pipe (8) is arranged on the outer side of the through hole in the top of the incubator fixing cover (1), and the position of the culture dish inlet pipe (8) corresponds to that of the moving pipe (11).
10. The periodic microorganism sampling and testing device as set forth in claim 1, wherein: the lever subassembly includes two rotation levers (34) and two lever guide holder (35), two the articulated lower extreme that sets up in the fixed pipe of culture dish (9), two of center that rotates lever (34) the outside parcel that rotates lever (34) is provided with barrier film (33), two the both ends that rotate lever (34) are provided with the columniform arch, and two lever guide holder (35) are the slope form and set up in the bottom that removes pipe (11), and the outer wall of lever guide holder (35) seted up with the protruding external shape assorted spout of rotation lever (34) one end.
CN202210484830.0A 2022-05-06 2022-05-06 Microorganism regular sampling detection device Withdrawn CN114774250A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202210484830.0A CN114774250A (en) 2022-05-06 2022-05-06 Microorganism regular sampling detection device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202210484830.0A CN114774250A (en) 2022-05-06 2022-05-06 Microorganism regular sampling detection device

Publications (1)

Publication Number Publication Date
CN114774250A true CN114774250A (en) 2022-07-22

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN202210484830.0A Withdrawn CN114774250A (en) 2022-05-06 2022-05-06 Microorganism regular sampling detection device

Country Status (1)

Country Link
CN (1) CN114774250A (en)

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Application publication date: 20220722