CN114764187A - Microscope assembly suitable for imaging of large-size sample - Google Patents
Microscope assembly suitable for imaging of large-size sample Download PDFInfo
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- CN114764187A CN114764187A CN202110504747.0A CN202110504747A CN114764187A CN 114764187 A CN114764187 A CN 114764187A CN 202110504747 A CN202110504747 A CN 202110504747A CN 114764187 A CN114764187 A CN 114764187A
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- 238000003384 imaging method Methods 0.000 title claims abstract description 78
- 238000006073 displacement reaction Methods 0.000 claims abstract description 13
- 229910001220 stainless steel Inorganic materials 0.000 claims description 8
- 239000010935 stainless steel Substances 0.000 claims description 8
- 239000000463 material Substances 0.000 claims description 3
- 239000000523 sample Substances 0.000 abstract description 65
- 230000015572 biosynthetic process Effects 0.000 abstract description 8
- 239000012472 biological sample Substances 0.000 abstract description 6
- 239000007788 liquid Substances 0.000 description 12
- 238000000034 method Methods 0.000 description 12
- 230000008569 process Effects 0.000 description 9
- 210000004556 brain Anatomy 0.000 description 3
- 210000000278 spinal cord Anatomy 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- 230000008901 benefit Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- QURLONWWPWCPIC-UHFFFAOYSA-N 2-(2-aminoethoxy)ethanol;3,6-dichloro-2-methoxybenzoic acid Chemical compound NCCOCCO.COC1=C(Cl)C=CC(Cl)=C1C(O)=O QURLONWWPWCPIC-UHFFFAOYSA-N 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 210000003792 cranial nerve Anatomy 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000002220 organoid Anatomy 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/24—Base structure
- G02B21/26—Stages; Adjusting means therefor
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
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- General Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Optics & Photonics (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Microscoopes, Condenser (AREA)
Abstract
The invention belongs to the technical field of fluorescence microscope imaging, and particularly relates to a microscope assembly suitable for imaging a large-size sample. The utility model provides a microscope component suitable for jumbo size sample formation of image, includes formation of image storehouse and sample frame, the sample frame sets up in the formation of image storehouse cavity, sample frame fixed connection is in a three-dimensional displacement platform. The invention provides a microscope component suitable for imaging a large-size sample, which is used for imaging a large-size transparent biological sample, adopts a large-size imaging cabin, is suitable for imaging a large sample of 20x80x26mm, simultaneously allows the sample to move in the range of 20x80x26mm, and firmly fixes the sample on a sample rack during long-time imaging and moving of a displacement table without changing the position of the sample.
Description
Technical Field
The invention belongs to the technical field of fluorescence microscope imaging, and particularly relates to a microscope assembly suitable for imaging a large-size sample.
Background
With the continuous and deep research of life science, the three-dimensional spatial information based on biological tissues also enters deep research, such as cranial nerves, organoids, vascularity, and the like. Methods for processing large-scale biological samples (whole mouse brain, spinal cord and the like) and methods for three-dimensional microscopic imaging are gradually emerging. Researchers adopt various transparentizing methods such as CUBIC, CLARITY, uDISCO/iDISCO, PEGASOS and the like to perform transparentizing treatment on large-scale biological samples of different types, and then use novel microscopes such as a light sheet illumination microscope and the like to rapidly obtain a three-dimensional image of a complete large-scale biological sample. The sample is fixed on the objective table of a microscope for imaging after being subjected to transparentization treatment, a simple mode is needed to fix the large sample on the objective table in the imaging process, and the position of the sample cannot be changed in the long-time imaging process so as to prevent the data precision from being influenced.
The traditional transparentizing sample can be only divided into a plurality of blocks to be imaged under the influence of the system imaging range in the imaging process, for example, a sample of brain connecting with spinal cord can only be divided into two samples of brain and spinal cord to be imaged respectively, and then the two samples are spliced. This process can lead to sample damage and loss of important data.
Disclosure of Invention
The utility model provides a microscope component suitable for jumbo size sample formation of image, includes formation of image storehouse and sample frame, the sample frame sets up in the formation of image storehouse cavity, sample frame fixed connection is in a three-dimensional displacement platform.
As a preferred technical scheme, the sample holder comprises a vertically arranged fixing part and a horizontally arranged loading part, wherein the fixing part is fixedly connected with one end of the loading part; the fixing part is provided with a through hole.
As a preferable technical solution, the loading part comprises a first loading part and a second loading part, the first loading part and the second loading part are fixedly connected through a connecting part, two ends of the connecting part are respectively fixedly connected with the end parts of the first loading part and the second loading part,
as a preferable technical solution, the connecting portion is vertically arranged.
As a preferable technical scheme, the lower surface of the carrying part is provided with an inward groove; the surface of the connecting part is provided with a through groove.
As a preferred technical scheme, one end of the second object carrying part is fixedly connected with the connecting part, and the other end of the second object carrying part is provided with a first threaded hole.
As a preferred technical solution, the imaging chamber includes a bottom plate and a side plate, and the side plate is fixedly disposed around the bottom plate.
As a preferred technical scheme, an inwardly recessed wedge-shaped groove is formed in the lower surface of the bottom surface, a second threaded hole is formed in the end portion of the wedge-shaped groove, and the second threaded hole penetrates through the side plate.
As a preferred technical scheme, be provided with two light trap on the curb plate, the light trap runs through the curb plate, two the light trap sets up relatively.
As a preferred technical scheme, the inner wall of the light hole is provided with a light transmitting mirror, and the light transmitting mirror is in threaded connection with the light hole.
Has the advantages that:
(1) the invention provides a microscope component suitable for imaging a large-size sample, which is used for imaging a large-size transparent biological sample, adopts a large-size imaging cabin, is suitable for imaging a large sample of 20x80x26mm, simultaneously allows the sample to move in the range of 20x80x26mm, and firmly fixes the sample on a sample rack during long-time imaging and moving of a displacement table without changing the position of the sample.
(2) The imaging bin can be fixedly connected with other parts at the bottom, such as a waste liquid pool, in a wedge-shaped connection mode by arranging the wedge-shaped groove at the bottom of the imaging bin, and the X-axis movement and rotation freedom degree and the Y-axis rotation freedom degree are limited in a wedge-shaped matched connection mode; meanwhile, the imaging bin and the waste liquid tank are fixedly connected through threads of threaded holes in the end part of the wedge-shaped groove, and the moving freedom degrees of the Y axis and the Z axis of the imaging bin are limited. Other components below the imaging chamber are stably and fixedly connected, and the stability of the imaging chamber in the imaging process is kept. Meanwhile, the wedge-shaped matched connection mode has both guiding and fixing functions, and the fixing bin can be very easily locked on the waste liquid pool.
(3) In order to bear a large-size sample and ensure the stability of the sample in the imaging process, the imaging frame made of stainless steel is adopted to ensure that the imaging frame is not subjected to remarkable stress deformation, the imaging liquid generally has corrosivity or toxicity, the imaging frame made of stainless steel can be prevented from being corroded and dissolved by the imaging liquid to pollute the imaging liquid, meanwhile, the surface of a stainless steel product is smooth and convenient to clean, and no toxic imaging liquid is left after the imaging frame is used, so that other damages are further caused.
In order to avoid adverse effects such as dust pollution and external light source intrusion, a fluorescence microscope is generally arranged in a sealed shell, which requires that all components have a volume as small as possible and ensure sufficient precision, the bearing capacity of a three-dimensional displacement platform with small volume and high precision is generally not high, especially the bearing capacity in the Z direction is not high, and a large-size sample is generally heavy, so that a sample frame is required to be as light as possible on the premise of ensuring stable carrying; according to the invention, the through groove is formed in the connecting part of the sample frame to reduce the weight, and the inward groove is formed in the lower surface of the object carrying part, so that unnecessary materials are removed on the premise of ensuring the structural strength of the sample frame, the weight is reduced as much as possible, the stable operation of the three-dimensional displacement platform is ensured, and the imaging precision is ensured.
(5) When the sample size is great, surpass the width size of sample frame, in order to ensure the formation of image testing process, the sample can not take place the position under the dead weight and remove, influences the formation of image data precision, has processed a second screw hole on the sample frame, and the second screw hole is used for connecting the spring leaf, utilizes the spring leaf to fix the sample centre gripping.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the embodiments or the prior art descriptions will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.
The drawings of the embodiments of the disclosure only relate to the structures related to the embodiments of the disclosure, and other structures can refer to the common design. In the drawings used to describe embodiments of the disclosure, the thickness of layers or regions are exaggerated or reduced for clarity, i.e., the drawings are not necessarily to scale.
FIG. 1 is a schematic structural view of a microscope assembly suitable for imaging large-size samples according to the present invention;
FIG. 2 is a schematic view of the structure of the upper surface of the sample holder
FIG. 3 is a schematic view of the structure of the lower surface of the sample holder;
FIG. 4 is a schematic structural view of an imaging cartridge;
FIG. 5 is a cross-sectional structural schematic of an imaging cartridge;
the device comprises an imaging bin, an imaging bin 11, a bottom plate, a wedge-shaped groove 111, a second threaded hole 112, a side plate 12, a light transmission hole 121, a sample holder 2, a fixing part 3, a through hole 31, a first object carrying part 41, a second object carrying part 42, a first threaded hole 421 and a connecting part 43.
Detailed Description
The invention will be further understood by reference to the following detailed description of preferred embodiments of the invention and the examples included therein.
The use of "preferred," "preferably," "more preferred," and the like, when describing embodiments of the present application, is intended to refer to embodiments of the invention that may provide certain benefits, under certain circumstances. However, other embodiments may be preferred, under the same or other circumstances. In addition, the recitation of one or more preferred embodiments does not imply that other embodiments are not useful, nor is it intended to exclude other embodiments from the scope of the invention.
In this document, relational terms such as first, second, and the like may be used solely to distinguish one entity from another entity without necessarily requiring or implying any actual such relationship or order between such entities. Furthermore, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a component, apparatus, or device that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such component, apparatus, or device.
When a component, element, or layer is referred to as being "on," "bonded to," "connected to," or "coupled to" another element or layer, it may be directly on, bonded to, connected to, or coupled to the other element, or layer, or intervening elements or layers may be present. In contrast, when an element is referred to as being "directly on," "directly coupled to," "directly connected to" or "directly coupled to" another element or layer, there may be no intervening elements or layers present. Other words used to describe the relationship between elements should be interpreted in a similar manner (e.g., "between … …" versus "directly between … …," "adjacent" versus "directly adjacent," etc.).
The microscope assembly suitable for imaging of the large-size sample shown in fig. 1-5 comprises an imaging chamber 1 and a sample holder 2, wherein the sample holder 2 is arranged in a cavity of the imaging chamber 1, and the sample holder 2 is fixedly connected to a three-dimensional displacement platform.
The three-dimensional displacement platform is any displacement platform capable of realizing a three-dimensional movement function, and the type and the kind of the displacement platform are not limited.
The sample holder 2 comprises a fixing part 3 which is vertically arranged and a loading part which is horizontally arranged, and the fixing part 3 is fixedly connected with one end of the loading part; the fixing portion 3 is provided with a through hole 31.
The through holes 31 are used for locking the sample holder 2 on the displacement table through screws, so that the sample position change caused by the movement of the sample holder is avoided.
The loading part comprises a first loading part 41 and a second loading part 42, the first loading part 41 and the second loading part 42 are fixedly connected through a connecting part 43, and two ends of the connecting part 43 are respectively fixedly connected with the end parts of the first loading part 41 and the second loading part 42; preferably, the connection portion 43 is vertically disposed.
The lower surface of the carrying part is provided with an inward groove; the surface of the connecting portion 43 is provided with a through groove.
One end of the second loading part 42 is fixedly connected with the connecting part 43, and the other end is provided with a first threaded hole 421.
The first threaded hole 421 is used for connecting a spring piece, and when the size of a sample is large and exceeds the width of the sample holder, the sample cannot move under the dead weight in the imaging test process to influence the imaging data precision, and the spring piece is used for clamping and fixing the sample.
The material of the sample holder 2 is stainless steel.
In order to bear a large-size sample and ensure the stability of the sample in the imaging process, the imaging frame made of stainless steel is adopted to ensure that the imaging frame is not subjected to remarkable stress deformation, the imaging liquid generally has corrosivity or toxicity, the imaging frame made of stainless steel can be prevented from being corroded and dissolved by the imaging liquid to pollute the imaging liquid, meanwhile, the surface of a stainless steel product is smooth and convenient to clean, and no toxic imaging liquid is left after the imaging frame is used, so that other damages are further caused.
The imaging bin 1 comprises a bottom plate 11 and a side plate 12, wherein the side plate 12 is fixedly arranged around the bottom plate 11; the lower surface of the bottom plate 11 is provided with an inward-recessed wedge-shaped groove 111, the end of the wedge-shaped groove 111 is provided with a second threaded hole 112, and the second threaded hole 112 penetrates through the side plate 12.
The length of the bottom plate 11 is 60-100 mm, the width of the bottom plate 11 is 40-50 mm, and the height of the side plate 12 is 20-30 mm.
The side plate 12 is provided with two light holes 121, the light holes 121 penetrate through the side plate 12, and the two light holes 121 are arranged oppositely.
The inner wall of the light hole 121 is provided with a light transmitting mirror, and the light transmitting mirror is in threaded connection with the light hole 121.
The working principle is as follows: the invention provides a microscope component suitable for imaging a large-size sample, which is used for imaging a large-size transparent biological sample, wherein a sample frame is used for bearing the large-size sample and driving the sample to move in an imaging bin by a three-dimensional displacement platform; the imaging liquid is arranged in the imaging bin, so that the sample is transparent, the light source is absorbed from the light hole, and the imaging test is completed by irradiating on the sample.
Finally, it should be noted that the above-mentioned contents are only used for illustrating the technical solutions of the present invention, and not for limiting the protection scope of the present invention, and that the simple modifications or equivalent substitutions of the technical solutions of the present invention by those of ordinary skill in the art can be made without departing from the spirit and scope of the technical solutions of the present invention.
Claims (10)
1. A microscope assembly suitable for imaging of a large-size sample is characterized by comprising an imaging bin (1) and a sample holder (2), wherein the sample holder (2) is arranged in a cavity of the imaging bin (1), and the sample holder (2) is fixedly connected to a three-dimensional displacement platform.
2. Microscope assembly suitable for imaging large-size samples according to claim 1, characterized in that the sample holder (2) comprises a vertically arranged fixing part (3), a horizontally arranged object carrying part, the fixing part (3) being fixedly connected with one end of the object carrying part; the fixing part (3) is provided with a through hole (31).
3. Microscope assembly according to claim 2, wherein the object carrying portion comprises a first object carrying portion (41) and a second object carrying portion (42), the first object carrying portion (41) and the second object carrying portion (42) are fixedly connected by a connecting portion (43), and two ends of the connecting portion (43) are fixedly connected with the ends of the first object carrying portion (41) and the second object carrying portion (42), respectively; preferably, the connecting portion (43) is vertically disposed.
4. A microscope assembly suitable for imaging large size samples according to claim 3 wherein the lower surface of the loading portion is provided with an inward recess; the surface of the connecting part (43) is provided with a through groove.
5. A microscope assembly suitable for imaging large-size samples according to claim 3 or 4, wherein one end of the second loading part (42) is fixedly connected with the connecting part (43), and the other end is provided with a first threaded hole (421).
6. A microscope assembly suitable for imaging large size samples according to claim 1 or 2 characterised in that the material of the sample holder (2) is stainless steel.
7. A microscope assembly suitable for imaging large size samples according to claim 1 or 2, wherein the imaging chamber (1) comprises a base plate (11) and a side plate (12), the side plate (12) being fixedly arranged around the base plate (11); the lower surface of the bottom plate (11) is provided with an inwards-recessed wedge-shaped groove (111), the end part of the wedge-shaped groove (111) is provided with a second threaded hole (112), and the second threaded hole (112) penetrates through the side plate (12).
8. The microscope assembly suitable for imaging a large-size sample according to claim 7, wherein the length of the bottom plate (11) is 60-100 mm, the width of the bottom plate (11) is 40-50 mm, and the height of the side plate (12) is 20-30 mm.
9. Microscope assembly suitable for imaging large-sized samples according to claim 7, characterized in that two light holes (121) are provided in the side plate (12), the light holes (121) extending through the side plate (12), the two light holes (121) being arranged opposite to each other.
10. The microscope assembly suitable for imaging of large-size samples according to claim 9, wherein the inner wall of the light-transmitting hole (121) is provided with a light-transmitting mirror, and the light-transmitting mirror is in threaded connection with the light-transmitting hole (121).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110504747.0A CN114764187A (en) | 2021-05-10 | 2021-05-10 | Microscope assembly suitable for imaging of large-size sample |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110504747.0A CN114764187A (en) | 2021-05-10 | 2021-05-10 | Microscope assembly suitable for imaging of large-size sample |
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| CN114764187A true CN114764187A (en) | 2022-07-19 |
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| CN202110504747.0A Pending CN114764187A (en) | 2021-05-10 | 2021-05-10 | Microscope assembly suitable for imaging of large-size sample |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2443364Y (en) * | 2000-09-29 | 2001-08-15 | 中国科学院低温技术实验中心 | Heat sink type low temp biosample table for scanning probe microscope |
| CN103403835A (en) * | 2011-03-02 | 2013-11-20 | 加登有限公司 | Microtome utilizing a movable knife in a retardation field scanning electron microscope and a retardation field scanning electron microscope including the same |
| CN110673323A (en) * | 2019-10-16 | 2020-01-10 | 西湖大学 | Microscope assembly |
| CN111381356A (en) * | 2018-12-27 | 2020-07-07 | 锘海生物科学仪器(上海)股份有限公司 | Microscope sample fixing frame and fixing method thereof |
| CN211669438U (en) * | 2020-04-29 | 2020-10-13 | 锘海生物科学仪器(上海)股份有限公司 | Sample fixing seat |
-
2021
- 2021-05-10 CN CN202110504747.0A patent/CN114764187A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2443364Y (en) * | 2000-09-29 | 2001-08-15 | 中国科学院低温技术实验中心 | Heat sink type low temp biosample table for scanning probe microscope |
| CN103403835A (en) * | 2011-03-02 | 2013-11-20 | 加登有限公司 | Microtome utilizing a movable knife in a retardation field scanning electron microscope and a retardation field scanning electron microscope including the same |
| CN111381356A (en) * | 2018-12-27 | 2020-07-07 | 锘海生物科学仪器(上海)股份有限公司 | Microscope sample fixing frame and fixing method thereof |
| CN110673323A (en) * | 2019-10-16 | 2020-01-10 | 西湖大学 | Microscope assembly |
| CN211669438U (en) * | 2020-04-29 | 2020-10-13 | 锘海生物科学仪器(上海)股份有限公司 | Sample fixing seat |
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