CN114749478A - Method for restoring lead-polluted soil - Google Patents
Method for restoring lead-polluted soil Download PDFInfo
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Images
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Soil Sciences (AREA)
- Environmental & Geological Engineering (AREA)
- Botany (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to a method for restoring lead-polluted soil, which comprises the steps of cultivating a plant variety capable of absorbing lead pollution in the soil, and irrigating amino acid water-soluble fertilizer to the root of the plant variety on the first set days after transplanting seedlings; on the second set of days after transplantation, the root was inoculated with a microbial inoculum, and the microorganisms of the microbial inoculum were named as one of QX8, QX13, and TS6, respectively. According to the technical scheme, the amino acid water-soluble fertilizer and the specific microbial agent are applied to the plants capable of absorbing the lead in the soil, and after the microbial agent is applied to the roots of the plants, substances for promoting the growth of the plants can be secreted on the one hand, and on the other hand, the microbial agent has the effect of promoting the transfer of the lead in the soil to the roots of the plants by secreting corresponding products, so that the enrichment capacity of the plants on the lead in the soil is improved.
Description
Technical Field
The invention belongs to the technical field of soil bioremediation, and particularly relates to a method for restoring lead-polluted soil.
Background
Lead is a common toxic element in heavy metal contaminated soil, and mainly enters the soil through human activities, such as mining, smelting processes, pesticide use and the like, so that the soil is polluted. Lead in the polluted soil enters the plant body through the root system to accumulate and produce toxic effects, such as slow growth and yellowing of the plant, influence absorption and transportation of the plant to essential elements, reduce the photosynthesis rate of the plant, and further endanger the health of animals and human beings through a food chain. Therefore, there is an increasing interest in developing safe, environmentally friendly, and economical methods to control and reduce heavy metal contamination of agricultural sites.
Meanwhile, the modification of the lead-polluted soil is also widely concerned, and compared with the traditional restoration technology, the phytoremediation is an environment-friendly and high-benefit in-situ treatment technology for the heavy metal-polluted soil, and has wide research prospects. However, the phytoremediation period is long, and the plant extraction capacity of the hyperaccumulative plants is limited by factors such as low biomass of overground parts, low bioavailability of metals, unsuitability for highly polluted sites, and the like, so that the large-scale application of the plants is limited.
In recent years, measures for improving phytoremediation by utilizing microorganisms, soil conditioners, chelating agents and the like are continuously mentioned, and the chelating agents such as EDTA, NTA and the like have the effects of increasing the effectiveness of soil heavy metals, improving the absorption and transportation of plants to the heavy metals and enhancing the phytoremediation efficiency, but the chelating agents often have the problems of difficulty in degradation, easiness in causing secondary pollution, high cost and the like, so that the large-scale application of the chelating agents is limited.
Moreover, the remediation of soil by using plants, microorganisms and the like is generally directed to the remediation of cadmium metal contaminated soil, and the remediation of lead contaminated soil by using microorganisms is a difficult point of soil remediation.
Patent publication No. CN110252801A, entitled method for repairing cadmium-contaminated soil, applies polyaspartic acid aqueous solution to solanum nigrum seedlings and applies microbial inoculum to roots, wherein the microbial inoculum is plant growth-promoting bacteria (PGPR), belongs to the genus Bacillus, and realizes the improvement of plant extraction efficiency by remarkably promoting the growth of solanum nigrum, but through analysis, the microbial inoculum actually promotes the biomass of plants to realize the absorption of cadmium in the soil.
Disclosure of Invention
The invention aims to provide a method for repairing lead-polluted soil, which solves the problems of difficulty in repairing lead-polluted soil, high cost and poor effect in the prior art.
A method for remediating lead-contaminated soil, comprising the steps of:
s1, cultivating a plant variety which can absorb lead pollution in soil, and planting seedlings of the plant variety which grow to be suitable for transplanting into the lead pollution soil needing to be repaired;
s2, irrigating amino acid water-soluble fertilizer to the roots of the seedlings on the first set days after the seedlings are transplanted;
s3, applying a microbial agent to the root of the seedling on the second set day after the seedling is transplanted, wherein the microbial agent has a nucleotide sequence shown as SEQ ID No.1 and named as QX8, and a nucleotide sequence shown as SEQ ID No.2 and named as QX13 or a nucleotide sequence shown as SEQ ID No.3 and named as one of TS 6;
and S4, harvesting the plants on the third set day after the transplanting of the seedlings.
Preferably, the plant variety in step S1 is solanum nigrum.
Preferably, the application amount of the amino acid water-soluble fertilizer in the step S2 is 3-10mg N/kg lead-contaminated soil.
Preferably, the first set of days is 15 days, the second set of days is 25 days, and the third set of days is 40 days.
Preferably, the microorganism QX8 and the microorganism QX13 are both derived from the rhizosphere soil of farmland plants near the Nanjing city Cyanea-silvestita zinc ore; the microorganism TS6 is derived from waste soil of Tokyo mountain copper mine in Nanjing.
Preferably, the dosage of the microbial inoculum is 30-100Ml/kg lead-polluted soil.
The invention has the beneficial effects that:
according to the technical scheme, the amino acid water-soluble fertilizer and the specific microbial inoculum are applied to the plants capable of absorbing lead in the soil, and after the microbial inoculum is applied to the roots of the plants, substances for promoting the growth of the plants can be secreted on the one hand, and on the other hand, the microbial inoculum has the effect of promoting the transfer of the lead in the soil to the roots of the plants by secreting corresponding products, so that the enrichment capacity of the plants on the lead in the soil is improved.
Drawings
FIG. 1 is a schematic illustration of the effect of combined application of nitrogen fertilizer and plant growth promoting bacteria on Pb content in the aerial and underground parts of Solanum nigrum;
FIG. 2 is a schematic diagram showing the effect of combined application of nitrogen fertilizer and plant growth-promoting bacteria on the total Pb extraction amount of the overground part and the root system of Solanum nigrum.
Detailed Description
The following embodiments are merely exemplary, and can be used to explain and illustrate the technical solutions of the present invention, and should not be construed as limiting the technical solutions of the present invention.
The microbial strains QX8 and QX13 used in the technical scheme of the application are screened from the rhizosphere soil of farmland plants near the Cyanea-silvestris lead zincite in Nanjing; the strain TS6 is screened from the waste soil of the Tokyo mountain copper mine in Nanjing City; all the materials are stored in the laboratory. Black fruit nightshade seeds are purchased in the natural breeding industry in Shandong Weifang City.
The heavy metal contaminated soil used in the test is collected in a farmland near the plumbum-zincite in the Xixia area of Nanjing, the contaminated farmland soil with the plough layer depth of 0-20cm is collected, the collected soil sample is naturally dried in the air, ground and finally sieved by a 10-mesh sieve for later use.
Selecting mature and plump black nightshade seeds, firstly using 5% NaClO for disinfection, then using deionized water for soaking for 6h, and naturally drying for later use. And flatly paving the sterilized vermiculite in a net basket, wherein the thickness of the vermiculite is 4-6cm, integrally placing the net basket in a turnover box, adding water into the turnover box, and uniformly sowing the solanum nigrum seeds into the turnover box after the vermiculite absorbs water to a saturated state. Placing into a constant temperature culture chamber at 25 deg.C, maintaining vermiculite water content at 80%, lighting for 16h, and darkness for 8h, and repeating the above steps. Transplanting when the seedlings grow to three leaves and one heart.
The treated heavy metal contaminated soil was loaded into plastic pots (diameter 15cm, height 11.5cm) each containing 1.0 kg. Combining an amino acid water-soluble fertilizer (AA) and a polyaspartic acid aqueous solution (PASP), 7 treatment groups were set: the method comprises the following steps of (1) not applying any novel nitrogen fertilizer and microbial inoculum (CK), wherein the treatment groups comprise AA + QX8, AA + QX13, AA + TS6, PASP + QX8, PASP + QX13 and PASP + TS6, wherein the 7 treatment groups are shown in horizontal coordinates in figures 1 and 2, and each treatment group is 4 repeated and completely randomly arranged. Transplanting 4 seedlings with good growth vigor and consistent size in each pot, moving the flowerpot into a greenhouse, and irrigating with deionized water to keep the water content of the matrix to be about 60% of the saturated water holding capacity; fertilizing 15 days after seedlings are transplanted, applying fertilizers in an amount of 5mg N/kg soil to each pot, dissolving the fertilizers in 15mL of deionized water, irrigating the roots of the solanum nigrum, and adding 15mL of deionized water to a control group (CK) without the fertilizers and the strains; after 25 days of transplanting, different microbial agents are applied, 50mL of bacterial liquid is applied to each pot, and 50mL of deionized water is added to a control group (CK). Plant samples and rhizosphere soil samples were collected for subsequent analysis at 40 days after transplantation.
Washing the plant sample with tap water to remove surface soil and residues; then washing with deionized water for three times; then the roots are put into 20mmol/L EDTA-Na2Soaking in the solution for 30min to remove heavy metals on the surface of plant root system, and washing with deionized water. Dividing the sample into an overground part and a root system, sucking water on the surface of the sample by using filter paper, drying the sample at 80 ℃ to constant weight, and measuring the dry weight of the overground part and the root system.
Plant and soil samples using guaranteed pure HClO4:HNO3(13:87, v/v) digesting the plant and soil samples, wherein the concentration of heavy metal Pb in the samples is coupled through inductanceAnd (4) measuring by a plasma mass spectrometer (ICP-MS). Blank and plant, soil standard GBW07603(GSV-2) and GBW07405(GSS-5) are respectively used as a negative control and a positive control in the sample digestion and heavy metal element content analysis and determination processes, so as to ensure the reliability of the whole analysis and determination process.
Calculating the heavy metal transfer coefficient (TF) in the plant body: the transfer coefficient is the heavy metal concentration (mg/kg) of the overground part of the plant/the heavy metal concentration (mg/kg) of the root system of the plant.
The effective state of the heavy metals in the plant rhizosphere soil is extracted by a DTPA solution, and the harvested black nightshade rhizosphere soil sample is naturally dried in the air, ground and sieved by a 60-mesh sieve. Weighing 2.0g of sieved soil, and adding 5mL of DTPA leaching liquor (0.005mol/L DTPA +0.01mol/L CaCl)2+0.1mol/L TEA). 20 ℃ and shaking at 200rpm for 2 h. Centrifuging at 8000r/min for 5min after oscillation, and measuring the effective concentration of heavy metal in soil with inductively coupled plasma emission spectrometer (ICP-OES).
Data were processed using Excel 2016 and SPSS25.0 and plotted against GraphPad Prism 6, and tested for significance by one-way anova (P <0.05) according to Duncan, with different letters indicating significant differences between treatments.
Compared with a control without plant growth-promoting bacteria and liquid amino acid fertilizer, the combined application of the nitrogen fertilizer and the microbial agent promotes the growth of the plants, the biomass of the overground part is 0.42-0.56 g/plant, and the biomass of the root system is 0.095-0.13 g; except for the combination of PASP + QX13 and PASP + TS6, the other combined treatments significantly increased the biomass of the aerial and underground parts of Solanum nigrum (FIG. 1). The growth promoting effect of the AA and the three plant growth promoting bactericides on the growth of the solanum nigrum is better than that of PASP combination; the growth promoting effect of the three strains on the black nightshade is as follows: QX8> QX13> TS 6. The growth promoting effect of the AA and QX8 microbial inoculum combination is optimal, so that the biomass of the overground part and the root system of the solanum nigrum is respectively increased by 1.3 times and 1.05 times.
Two nitrogen fertilizers were applied in combination with different microbial agents to solanum nigrum growing in contaminated soil. AA + QX13 has the best effect of promoting the Pb content of the overground part, the Pb content is increased by 15%, the Pb content of the solanum nigrum is reduced by combining PASP + QX13 and PASP + TS6, and the lead concentration of the underground part in each group is far higher than that of the overground part.
As shown in fig. 2, the combined application of nitrogen fertilizer and microbial inoculum significantly increased the total amount of Pb in solanum nigrum, showing a similar trend to heavy metal concentrations. The total Pb extraction amount of the overground part is 1.70-3.08 times of that of the contrast overground part, and the two treatments of PASP + QX13 and PASP + TS6 have no obvious promotion effect on the total Pb extraction amount of the overground part; the total extraction amount of Pb in the root system is 1.21-1.80 times of that in the control root system, and the total extraction amount of Pb in the root system is higher than that in the overground part. And the AA + QX13 treatment has the best effect on transferring Pb, the transfer coefficient is improved by 2.18 times, and Pb is mainly accumulated in the root system of the black nightshade.
TABLE 1 Effect of the Combined application of Nitrogen fertilizers and plant growth-promoting bacteria on the Pb transfer coefficient of Solanum nigrum
| Treatment of | Coefficient of Pb transfer |
| CK | 0.11±0.02b |
| AA+QX8 | 0.10±0.01b |
| AA+QX13 | 0.24±0.08a |
| AA+TS6 | 0.19±0.06a |
| PASP+QX8 | 0.12±0.03ab |
| PASP+QX13 | 0.09±0.01b |
| PASP+TS6 | 0.13±0.05ab |
The test strains are separated from heavy metal heavily-polluted soil, have certain tolerance capacity to Pb, and have plant growth promotion properties of producing 1-aminocyclopropane-carboxylic Acid (ACC) deaminase, indole-3-acetic acid (IAA), siderophore, phosphate solubilization activity and the like. And Polyaspartic Acid (PASP) and liquid amino acid fertilizer (AA) as two novel green nitrogen fertilizers can improve the nitrogen level in plants, promote the growth of the plants and improve the crop yield. However, the application of the combination of the two to the enhanced plant extraction has not been reported. In the embodiment, the biomass of the solanum nigrum is increased by the combination of the AA, the PASP and the 6 treatment methods of the plant growth promoting bacterial strains, wherein the combination mode of the AA fertilizer and the bacterial agent shows a better solanum nigrum growth promoting effect. It is presumed that this is because AA is a complex amino acid, free amino acids chelate essential trace elements of plants, and enhance the absorption of mineral nutrients in soil, and on the other hand, free amino acids are easily absorbed and utilized by plants, thereby simplifying the N assimilation process of plants, more effectively promoting the growth of restored plants, and having a better effect of improving soil microorganisms.
The plant extraction of the heavy metal in the soil by the plant mainly depends on the absorption capacity and the transfer efficiency of the root system to the heavy metal, so that the strong root system and the high effectiveness of the heavy metal in the soil are beneficial to the plant to absorb the heavy metal in the soil. The nitrogen fertilizer can reduce the toxic action of heavy metals on plants by influencing physical and chemical factors such as soil pH, organic acid content and the like, and improve the bioavailability and mobility of the heavy metals in the soil. The microorganisms can dissolve heavy metals in the soil by secreting extracellular enzymes, organic acids and the like, so that the effectiveness of the heavy metals in the soil is improved, the toxicity of the heavy metals to plants can be reduced, the growth of the plants is promoted, and the extraction efficiency of the plants to the heavy metals is improved. In the test, the content of the heavy metal effective state in the soil is improved by combined application, wherein the AA + QX13 combination has a better promotion effect on the Pb content, and the Pb content is favorably absorbed by the black nightshade. The advantages of combined application are mainly embodied in that the heavy metal content and the total extraction amount of tissues in the solanum nigrum body are improved, and the Pb total extraction amount of the overground part of the solanum nigrum is 2-3 times higher than that of a contrast group after the combined application, because the novel nitrogen fertilizer applied firstly is gradually degraded by soil microorganisms, and the plant growth promoting bacteria agent applied subsequently can immediately make up the actual problem of the reduction of the lead extraction efficiency of the solanum nigrum caused by the degradation of the novel nitrogen fertilizer. The sequential connection of the two application sequences can effectively prolong the high-efficiency extraction efficiency of the black nightshade on the time scale; so that the extraction efficiency and effect of the lead from the black nightshade are more durable and efficient. In addition, the promoting effect of different treatment combinations of the nitrogen fertilizer and the microbial inoculum on Pb extraction of the solanum nigrum is different. Pb accumulates mainly in the plant root system, which is mainly associated with low mobility of Pb in the soil, lower transport capacity from the root system to the overground part.
Table 2 Effect of combined application of nitrogenous fertilizer and plant growth-promoting bacteria on Pb available state content of Solanum nigrum rhizosphere soil
Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those of ordinary skill in the art that: it is to be understood that modifications may be made to the technical solutions described in the foregoing embodiments, or equivalents may be substituted for some of the technical features thereof, but such modifications or substitutions do not depart from the spirit and scope of the technical solutions of the embodiments of the present invention.
Sequence listing
<110> tobacco industry, Limited liability company, in Henan
<120> method for restoring lead polluted soil
<130> WPC220949
<141> 2022-04-02
<160> 3
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1421
<212> DNA
<213> Artificial sequence (QX8)
<400> 1
acatgcaagt cgagcgaatg gattaagagc ttgctcttat gaagttagcg gcggacgggt 60
gagtaacacg tgggtaacct gcccataaga ctgggataac tccgggaaac cggggctaat 120
accggataac attttgaact gcatggttcg aaattgaaag gcggcttcgg ctgtcactta 180
tggatggacc cgcgtcgcat tagctagttg gtgaggtaac ggctcaccaa ggcaacgatg 240
cgtagccgac ctgagagggt gatcggccac actgggactg agacacggcc cagactccta 300
cgggaggcag cagtagggaa tcttccgcaa tggacgaaag tctgacggag caacgccgcg 360
tgagtgatga aggctttcgg gtcgtaaaac tctgttgtta gggaagaaca agtgctagtt 420
gaataagctg gcaccttgac ggtacctaac cagaaagcca cggctaacta cgtgccagca 480
gccgcggtaa tacgtaggtg gcaagcgtta tccggaatta ttgggcgtaa agcgcgcgca 540
ggtggtttct taagtctgat gtgaaagccc acggctcaac cgtggagggt cattggaaac 600
tgggagactt gagtgcagaa gaggaaagtg gaattccatg tgtagcggtg aaatgcgtag 660
agatatggag gaacaccagt ggcgaaggcg actttctggt ctgtaactga cactgaggcg 720
cgaaagcgtg gggagcaaac aggattagat accctggtag tccacgccgt aaacgatgag 780
tgctaagtgt tagagggttt ccgcccttta gtgctgaagt taacgcatta agcactccgc 840
ctggggagta cggccgcaag gctgaaactc aaaggaattg acgggggccc gcacaagcgg 900
tggagcatgt ggtttaattc gaagcaacgc gaagaacctt accaggtctt gacatcctct 960
gaaaacccta gagatagggc ttctccttcg ggagcagagt gacaggtggt gcatggttgt 1020
cgtcagctcg tgtcgtgaga tgttgggtta agtcccgcaa cgagcgcaac ccttgatctt 1080
agttgccatc attaagttgg gcactctaag gtgactgccg gtgacaaacc ggaggaaggt 1140
ggggatgacg tcaaatcatc atgcccctta tgacctgggc tacacacgtg ctacaatgga 1200
cggtacaaag agctgcaaga ccgcgaggtg gagctaatct cataaaaccg ttctcagttc 1260
ggattgtagg ctgcaactcg cctacatgaa gctggaatcg ctagtaatcg cggatcagca 1320
tgccgcggtg aatacgttcc cgggccttgt acacaccgcc cgtcacacca cgagagtttg 1380
taacacccga agtcggtggg gtaacctttt ggagccagcc g 1421
<210> 2
<211> 1419
<212> DNA
<213> Artificial sequence (QX13)
<400> 2
tacatgcagt cgagcggacc gacgggagct tgctccctta ggtcagcggc ggacgggtga 60
gtaacacgtg ggtaacctgc ctgtaagact gggataactc cgggaaaccg gggctaatac 120
cggatgcttg attgaaccgc atggttcaat cataaaaggt ggcttttagc taccacttac 180
agatggaccc gcggcgcatt agctagttgg tgaggtaacg gctcaccaag gcgacgatgc 240
gtagccgacc tgagagggtg atcggccaca ctgggactga gacacggccc agactcctac 300
gggaggcagc agtagggaat cttccgcaat ggacgaaagt ctgacggagc aacgccgcgt 360
gagtgatgaa ggttttcgga tcgtaaaact ctgttgttag ggaagaacaa gtaccgttcg 420
aatagggcgg caccttgacg gtacctaacc agaaagccac ggctaactac gtgccagcag 480
ccgcggtaat acgtaggtgg caagcgttgt ccggaattat tgggcgtaaa gcgcgcgcag 540
gcggtttctt aagtctgatg tgaaagcccc cggctcaacc ggggagggtc attggaaact 600
ggggaacttg agtgcagaag aggagagtgg aattccacgt gtagcggtga aatgcgtaga 660
gatgtggagg aacaccagtg gcgaaggcga ctctctggtc tgtaactgac gctgaggcgc 720
gaaagcgtgg ggagcgaaca ggattagata ccctggtagt ccacgccgta aacgatgagt 780
gctaagtgtt agagggtttc cgccctttag tgctgcagca aacgcattaa gcactccgcc 840
tggggagtac ggtcgcaaga ctgaaactca aaggaattga cgggggcccg cacaagcggt 900
ggagcatgtg gtttaattcg aagcaacgcg aagaacctta ccaggtcttg acatcctctg 960
acaaccctag agatagggct tccccttcgg gggcagagtg acaggtggtg catggttgtc 1020
gtcagctcgt gtcgtgagat gttgggttaa gtcccgcaac gagcgcaacc cttgatctta 1080
gttgccagca ttcagttggg cactctaagg tgactgccgg tgacaaaccg gaggaaggtg 1140
gggatgacgt caaatcatca tgccccttat gacctgggct acacacgtgc tacaatgggc 1200
agaacaaagg gcagcgaagc cgcgaggcta agccaatccc acaaatctgt tctcagttcg 1260
gatcgcagtc tgcaactcga ctgcgtgaag ctggaatcgc tagtaatcgc ggatcagcat 1320
gccgcggtga atacgttccc gggccttgta cacaccgccc gtcacaccac gagagtttgt 1380
aacacccgaa gtcggtgagg taacctttgg agccagccg 1419
<210> 3
<211> 1369
<212> DNA
<213> Artificial sequence (TS6)
<400> 3
ggttactcca ccgacttcgg gtgttacaaa ctctcgtggt gtgacgggcg gtgtgtacaa 60
ggcccgggaa cgtattcacc gcggcatgct gatccgcgat tactagcgat tccagcttca 120
tgtaggcgag ttgcagccta caatccgaac tgagaatggt tttatgggat tggcttgacc 180
tcgcggtctt gcagcccttt gtaccatcca ttgtagcacg tgtgtagccc aggtcataag 240
gggcatgatg atttgacgtc atccccacct tcctccggtt tgtcaccggc agtcacctta 300
gagtgcccaa ctaaatgctg gcaactaaga tcaagggttg cgctcgttgc gggacttaac 360
ccaacatctc acgacacgag ctgacgacaa ccatgcacca cctgtcactc tgtcccccga 420
aggggaacgc tctatctcta gagttgtcag aggatgtcaa gacctggtaa ggttcttcgc 480
gttgcttcga attaaaccac atgctccacc gcttgtgcgg gcccccgtca attcctttga 540
gtttcagtct tgcgaccgta ctccccaggc ggagtgctta atgcgttagc tgcagcacta 600
aagggcggaa accctctaac acttagcact catcgtttac ggcgtggact accagggtat 660
ctaatcctgt ttgctcccca cgctttcgcg cctcagcgtc agttacagac caaaaagccg 720
ccttcgccac tggtgttcct ccacatctct acgcatttca ccgctacacg tggaattccg 780
cttttctctt ctgcactcaa gttccccagt ttccaatgac cctccacggt tgagccgtgg 840
gctttcacat cagacttaag aaaccgcctg cgcgcgcttt acgcccaata attccggata 900
acgcttgcca cctacgtatt accgcggctg ctggcacgta gttagccgtg gctttctggt 960
taggtaccgt caaggtacga gcagttactc tcgtacttgt tcttccctaa caacagagtt 1020
ttacgacccg aaagccttca tcactcacgc ggcgttgctc cgtcagactt tcgtccattg 1080
cggaagattc cctactgctg cctcccgtag gagtctgggc cgtgtctcag tcccagtgtg 1140
gccgatcacc ctctcaggtc ggctatgcat cgttgccttg gtgagccgtt acctcaccaa 1200
ctagctaatg caccgcgggc ccatctgtaa gtgatagccg aaaccatctt tcaatcatct 1260
cccatgaagg agaagatcct atccggtatt agcttcggtt tcccgaagtt atcccagtct 1320
tacaggcagg ttgcccacgt gttactcacc cgtccgccgc taacgtcat 1369
Claims (6)
1. The method for restoring the lead-polluted soil is characterized by comprising the following steps of:
s1, cultivating a plant variety which can absorb lead pollution in soil, and planting seedlings of the plant variety which grow to be suitable for transplanting into the lead pollution soil needing to be repaired;
s2, irrigating amino acid water-soluble fertilizer to the roots of the seedlings on the first set days after the seedlings are transplanted;
s3, applying a microbial agent to roots of seedlings on the second set day after the seedlings are transplanted, wherein the nucleotide sequence of the microorganism of the microbial agent is shown as SEQ ID NO.1 and is named as QX8, the nucleotide sequence is shown as SEQ ID NO.2 and is named as QX13, or the nucleotide sequence is shown as SEQ ID NO.3 and is named as one of TS 6;
and S4, harvesting the plants on the third set day after the transplanting of the seedlings.
2. The method for remediating lead-contaminated soil as recited in claim 1, wherein the plant species in step S1 is solanum nigrum.
3. The method for remediating lead-contaminated soil as recited in claim 1, wherein the amount of the water-soluble fertilizer of amino acids applied in step S2 is 3 to 10mg N/kg lead-contaminated soil.
4. The method for remediating lead-contaminated soil as recited in claim 1, wherein the first set of days is 15 days, the second set of days is 25 days, and the third set of days is 40 days.
5. The method for remediating lead-contaminated soil as recited in claim 1, wherein microorganism QX8 and microorganism QX13 are both derived from the rhizosphere soil of a farmland plant near Nanjing City Cyanea-silvestita; the microorganism TS6 is derived from waste soil of Tokyo mountain copper mine in Nanjing.
6. The method for remediating lead-contaminated soil as recited in claim 1, wherein the amount of the microbial agent is 30 to 100Ml/kg of lead-contaminated soil.
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