CN114748218A - Porous artificial vertebral body and preparation method thereof - Google Patents

Porous artificial vertebral body and preparation method thereof Download PDF

Info

Publication number
CN114748218A
CN114748218A CN202111400620.0A CN202111400620A CN114748218A CN 114748218 A CN114748218 A CN 114748218A CN 202111400620 A CN202111400620 A CN 202111400620A CN 114748218 A CN114748218 A CN 114748218A
Authority
CN
China
Prior art keywords
porous
main body
body structure
artificial vertebral
vertebral body
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202111400620.0A
Other languages
Chinese (zh)
Inventor
张大琛
杨景周
陈海深
杨景浩
张树培
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shenzhen Dazhou Medical Technology Co ltd
Original Assignee
Shenzhen Dazhou Medical Technology Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shenzhen Dazhou Medical Technology Co ltd filed Critical Shenzhen Dazhou Medical Technology Co ltd
Priority to CN202111400620.0A priority Critical patent/CN114748218A/en
Publication of CN114748218A publication Critical patent/CN114748218A/en
Pending legal-status Critical Current

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
    • A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
    • A61F2/02—Prostheses implantable into the body
    • A61F2/30—Joints
    • A61F2/44—Joints for the spine, e.g. vertebrae, spinal discs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
    • A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
    • A61F2/02—Prostheses implantable into the body
    • A61F2/30—Joints
    • A61F2/30767—Special external or bone-contacting surface, e.g. coating for improving bone ingrowth
    • A61F2/30771—Special external or bone-contacting surface, e.g. coating for improving bone ingrowth applied in original prostheses, e.g. holes or grooves
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
    • A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
    • A61F2/02—Prostheses implantable into the body
    • A61F2/30—Joints
    • A61F2/3094—Designing or manufacturing processes
    • A61F2/30942—Designing or manufacturing processes for designing or making customized prostheses, e.g. using templates, CT or NMR scans, finite-element analysis or CAD-CAM techniques
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
    • A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
    • A61F2/02—Prostheses implantable into the body
    • A61F2/30—Joints
    • A61F2/30767—Special external or bone-contacting surface, e.g. coating for improving bone ingrowth
    • A61F2/30771—Special external or bone-contacting surface, e.g. coating for improving bone ingrowth applied in original prostheses, e.g. holes or grooves
    • A61F2002/30772—Apertures or holes, e.g. of circular cross section
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
    • A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
    • A61F2/02—Prostheses implantable into the body
    • A61F2/30—Joints
    • A61F2/30767—Special external or bone-contacting surface, e.g. coating for improving bone ingrowth
    • A61F2002/3093—Special external or bone-contacting surface, e.g. coating for improving bone ingrowth for promoting ingrowth of bone tissue
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
    • A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
    • A61F2/02—Prostheses implantable into the body
    • A61F2/30—Joints
    • A61F2/3094—Designing or manufacturing processes
    • A61F2002/30985—Designing or manufacturing processes using three dimensional printing [3DP]

Abstract

The invention provides a porous artificial vertebral body and a preparation method thereof, belonging to the technical field of biomedical materials. The porous artificial vertebral body comprises a porous main body structure (1), wherein the porosity of the porous main body structure is 50-80%; the porous body structure (1) has an upper and a lower surface and four side surfaces; a hollow bone grafting channel (2) is arranged in the porous main body structure (1), and the bone grafting channel penetrates through the upper surface and the lower surface of the porous main body structure (1); and the solid reinforcing ribs (3) vertically penetrate through the upper surface and the lower surface of the porous main body structure (1). The porous artificial vertebral body provided by the invention has high porosity, and is beneficial to the fusion and growth of new bone tissues; the porous artificial vertebral body is characterized in that the porous main body structure (1) is used as a main body of the artificial vertebral body, the solid reinforcing ribs (3) are arranged in the porous main body structure (1), and the mechanical property of the porous artificial vertebral body can be matched with natural bones while the strength of the porous artificial vertebral body is ensured.

Description

Porous artificial vertebral body and preparation method thereof
Technical Field
The invention relates to the technical field of biomedical materials, in particular to a porous artificial vertebral body and a preparation method thereof.
Background
Trauma, disease, or congenital factors can cause a variety of spinal injuries and deformities that ultimately can result in impairment of vertebral body function. Spinal injuries can be traumatic, such as vertebral body fractures, or non-traumatic deformities caused by tumors or bony degeneration of vertebral bodies. In most treatments of spinal injuries, an anterior reconstruction of the spine is required, most commonly a total resection of the damaged vertebral body. In a typical anterior procedure, a partial or total surgical resection is made of the vertebral body and two adjacent intervertebral discs, followed by artificial vertebral body replacement in the void created after removal of the vertebral body and intervertebral discs.
The existing artificial vertebral body is an artificial vertebral body integrated with a personalized plate body based on 3D printing, such as patent CN106923941A, but only one side surface of the artificial vertebral body is of a void structure, the porosity is low, and only limited bone ingrowth is allowed; and the structure has the elasticity modulus far higher than that of human bones, and the mechanical property is not matched with that of natural bones, so that the stress shielding phenomenon can be generated, and the bone absorption and the bone dissolution are caused.
Disclosure of Invention
In view of the above, the present invention aims to provide a porous artificial vertebral body and a preparation method thereof. The porous artificial vertebral body provided by the invention has mechanical properties matched with natural bones and is beneficial to the fusion and ingrowth of bone tissues.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a porous artificial vertebral body which comprises a porous main body structure 1, wherein the porosity of the porous main body structure 1 is 50-80%; the porous main body structure 1 is provided with an upper surface, a lower surface and four side surfaces, and the upper surface and the lower surface of the porous main body structure 1 are bone connection surfaces; a hollow bone grafting channel 2 is arranged in the porous main body structure 1 and penetrates through the upper surface and the lower surface of the porous main body structure 1;
and the solid reinforcing ribs 3 vertically penetrate through the upper surface and the lower surface of the porous main body structure 1.
Preferably, the porous artificial vertebral body is made of pure tantalum or tantalum alloy.
Preferably, the upper surface and the lower surface of the porous main body structure 1 form an included angle of 0-7 degrees.
Preferably, one side surface of the porous main body structure 1 is an outer convex arc surface, and the other three side surfaces are planes; the upper and lower surfaces of the porous main body structure 1 are wave-shaped surfaces.
Preferably, the outer convex arc-shaped surface of the porous main body structure 1 is provided with an instrument hole 4, and the instrument hole 4 is communicated with the hollow bone grafting channel 2.
Preferably, the number of the solid reinforcing ribs 3 is 4, and the cross sections of the 4 solid reinforcing ribs 3 coincide with the diagonal lines of the upper surface and the lower surface of the porous main body structure 1.
Preferably, the pore diameter of the porous main body structure 1 is 300-1500 μm, and the filament diameter is 200-350 μm.
The invention provides a preparation method of the porous artificial vertebral body, which comprises the following steps:
preparing the porous artificial vertebral body in an additive manufacturing mode.
Preferably, the additive manufacturing mode is a powder laser melting method, and the parameters of the powder laser melting method include:
the laser power is 150-350W;
the scanning speed is 100-500 mm/s;
the scanning line interval is 0.05-0.15 mm:
the temperature of the substrate is 100-200 ℃;
the thickness of the slicing layer is 0.02-0.05 mm.
Preferably, the additive manufacturing mode is a powder bed electron beam melting method, and the parameters of the powder bed electron beam melting method include:
the preheating temperature is 750-1500 ℃:
the scanning current is 2-20 mA;
the scanning speed is 1.0 × 104～1.0×106mm/s；
The thickness of the slicing layer is 20-100 μm.
The invention provides a porous artificial vertebral body which comprises a porous main body structure 1, wherein the porosity of the porous main body structure 1 is 50-80%; the porous main body structure 1 is provided with an upper surface, a lower surface and four side surfaces, and the upper surface and the lower surface of the porous main body structure 1 are bone connection surfaces; a hollow bone grafting channel 2 is arranged in the porous main body structure 1 and penetrates through the upper surface and the lower surface of the porous main body structure 1; and the solid reinforcing ribs 3 vertically penetrate through the upper surface and the lower surface of the porous main body structure 1. The porous artificial vertebral body provided by the invention has high porosity, and is beneficial to the fusion and growth of new bone tissues; according to the invention, the porous main body structure 1 is used as a main body of the artificial vertebral body, and the solid reinforcing ribs 3 are arranged in the porous main body structure 1, so that the mechanical property of the porous artificial vertebral body can be matched with natural bone while the strength of the porous artificial vertebral body is ensured. The porous artificial vertebral body provided by the invention has the advantages that the compressive strength is 20-80 MPa, the bending strength is 25-120 MPa, the maximum torque is 20-100N cm, the elastic modulus is 1-4 GPa, and the mechanical property is matched with that of natural bone, so that the stress shielding can be effectively reduced, and the bone dissolution phenomenon can be reduced.
Furthermore, the porous artificial vertebral body provided by the invention is made of pure tantalum or tantalum alloy, is very stable in a human tissue environment and has excellent biocompatibility.
The invention provides the preparation method of the porous artificial vertebral body, the porous artificial vertebral body is prepared by adopting an additive manufacturing mode, the precision is high, and the industrial batch production is easy to realize.
Drawings
FIG. 1 is a schematic view of the structure of a porous artificial vertebral body according to the present invention;
FIG. 2 is a front view of the porous artificial vertebral body of the present invention;
FIG. 3 is a left side view of the porous artificial vertebral body of the present invention;
FIG. 4 is a top view of a porous artificial vertebral body of the present invention;
FIG. 5 is a partial optical micrograph of the porous host structure 1;
FIG. 6 is an SEM image of a porous host structure 1;
FIG. 7 is a compression curve of an equivalent test piece of porous tantalum of 60% porosity according to the present invention;
FIG. 8 is a bending curve of an equivalent test piece of porous tantalum of 60% porosity according to the present invention;
FIG. 9 is a torsion curve of an equivalent test piece of porous tantalum of 60% porosity according to the present invention;
FIG. 10 is a compression fatigue curve of an equivalent test piece of porous tantalum with 60% porosity according to the present invention;
FIG. 11 is a graph showing the results of a staining experiment for co-culture of viable and dead cells with porous tantalum and HUVEC according to the present invention;
in FIGS. 1 to 4, 1 is a porous main body structure, 2 is a hollow bone grafting channel, 3 is a solid reinforcing rib, 4 is an instrument hole, and 5 is an annular reinforcing rib.
Detailed Description
The invention provides a porous artificial vertebral body which comprises a porous main body structure 1, wherein the porosity of the porous main body structure 1 is 50-80%; the porous main body structure 1 is provided with an upper surface, a lower surface and four side surfaces, and the upper surface and the lower surface of the porous main body structure 1 are bone connection surfaces; a hollow bone grafting channel 2 is arranged in the porous main body structure 1 and penetrates through the upper surface and the lower surface of the porous main body structure 1;
and the porous main body structure also comprises a plurality of solid reinforcing ribs 3 vertically penetrating through the upper surface and the lower surface of the porous main body structure 1.
The porous artificial vertebral body provided by the invention comprises a porous main body structure 1. In the present invention, the porosity of the porous body structure 1 is 50 to 80%, preferably 70 to 75%. In the invention, the pore diameter of the porous main body structure 1 is preferably 300-1500 μm, more preferably 300-800 μm, and further preferably 400-600 μm; the silk diameter of the porous main body structure 1 is preferably 200-350 μm, and more preferably 250-300 μm.
In the present invention, the porous body structure 1 has upper and lower surfaces and four sides, and is shaped like a rectangular parallelepiped. In the invention, the height of the porous main body structure 1 is 17-62 mm, preferably 25-50 mm; every 1.5mm is a specification, the length of the upper surface section and the lower surface section is preferably 11-14 mm, and the width is preferably 11-14 mm.
In the present invention, the upper and lower surfaces of the porous body structure 1 are bone connection surfaces; in the present invention, the upper and lower surfaces of the porous main body structure 1 preferably form an included angle of 0 to 7 °, and more preferably 3 to 5 °. In the present invention, when the angle between the upper and lower surfaces of the porous body structure 1 is 0 °, the upper and lower surfaces are parallel.
In the present invention, the upper and lower surfaces of the porous body structure 1 are preferably wavy surfaces. In the invention, the crest interval (or trough interval) of the wavy surface is preferably 1-1.5 mm, and the wave depth is preferably 0.5-1 mm.
In the present invention, one side of the porous main body structure 1 is preferably an outer convex arc-shaped surface, and the remaining three sides are preferably flat surfaces. In the invention, the cambered surface radius of the convex cambered surface is preferably 12-16 mm.
In the invention, a hollow bone grafting channel 2 is arranged in the porous main body structure 1, and the bone grafting channel penetrates through the upper surface and the lower surface of the porous main body structure 1. In the invention, the cross section of the hollow bone grafting channel 2 is preferably a rounded rectangle, the length of the rounded rectangle is preferably 6-8 mm, the width of the rounded rectangle is preferably 4-6 mm, and the radius of a rounded corner is preferably 1-1.5 mm. In the present invention, the diagonal centers of the cross-section of the hollow bone graft passage 2 preferably coincide with the diagonal centers of the upper and lower surfaces of the porous body structure 1. In the present invention, the distance from the edge of the hollow bone grafting passage 2 to the side wall of the porous main body structure 1 is preferably 2 to 3mm, and more preferably 2.5 mm.
In the invention, the outer convex arc surface of the porous main body structure 1 is provided with an instrument hole 4, and the instrument hole 4 is communicated with the hollow bone grafting channel 2. In the invention, the difference between the height and the width of the instrument hole 4 is preferably more than or equal to 2mm, so that the instrument hole is convenient to clamp in the operation process. As a specific embodiment of the present invention, the instrument hole 4 has a height of 6mm and a width of 3 mm.
In the invention, the specific opening position of the instrument hole 4 is designed correspondingly according to the actual situation.
The porous artificial vertebral body provided by the invention also comprises a plurality of solid reinforcing ribs 3 vertically penetrating through the upper surface and the lower surface of the porous main body structure 1. In the present invention, the number of the solid reinforcing ribs 3 is preferably 4, and the cross section of the 4 solid reinforcing ribs 3 coincides with the diagonal lines of the upper and lower surfaces of the porous main body structure 1. In the invention, the thickness of the solid reinforcing rib 3 is preferably 0.5-1.5 mm, and more preferably 1 mm.
In the present invention, when the outer convex arc-shaped surface of the porous main body structure 1 is provided with the instrument hole 4, an annular reinforcing rib 5 is preferably arranged around the instrument hole 4, and the annular reinforcing rib 5 is preferably solid. In the present invention, the thickness of the annular bead 5 is preferably 0.5 to 1.5mm, and more preferably 1 mm.
In the invention, the porous artificial cone is made of pure tantalum or tantalum alloy. In the invention, the pure tantalum is preferably medical pure tantalum, and the purity is preferably 99.99%. The tantalum alloy is preferably tantalum-zirconium alloy, and the mass percentage of tantalum in the tantalum alloy is preferably 80-95%. More preferably 85 to 90%.
In the invention, the compressive strength of the porous artificial vertebral body is 20-80 MPa, the bending strength is 25-120 MPa, the maximum torque is 20-100N-cm, the elastic modulus is 1-4 GPa, and the mechanical property is matched with that of natural bone, so that stress shielding can be effectively reduced, and the bone dissolution phenomenon can be reduced.
As an embodiment of the invention, the porous artificial vertebral body has a structure as shown in figure 1, a front view as shown in figure 2, a side view as shown in figure 3 and a top view as shown in figure 4. In FIGS. 1 to 4, 1 is a porous main body structure, 2 is a hollow bone grafting channel, 3 is a solid reinforcing rib, 4 is an instrument hole, and 5 is an annular reinforcing rib.
The invention provides a preparation method of the porous artificial vertebral body, which comprises the following steps:
and preparing the porous artificial vertebral body by adopting an additive manufacturing mode.
In the present invention, the additive manufacturing method is preferably a powder bed laser melting method or a powder bed electron beam melting method.
In the present invention, the parameters of the powder laser melting method preferably include:
the laser power is 150-350W, and more preferably 200-300W;
the scanning speed is 100-500 mm/s, and more preferably 200-400 mm/s;
the scanning line interval is 0.05-0.15 mm, more preferably 0.08-0.12 mm:
the temperature of the substrate is 100-200 ℃, and more preferably 120-160 ℃;
the thickness of the slicing layer is 0.02-0.05 mm, and more preferably 0.03-0.04 mm.
In the present invention, the parameters of the powder bed electron beam melting method preferably include:
the preheating temperature is 750-1500 ℃, and more preferably 1000-1200 ℃:
the scanning current is 2-20 mA, and more preferably 5-15 mA;
the scanning speed is 1.0 × 104～1.0×106mm/s, more preferably 1.0X 105mm/s；
The thickness of the slicing layer is 20 to 100 μm, and more preferably 40 to 80 μm.
In the present invention, the method for preparing the porous artificial vertebral body by using the additive manufacturing method preferably comprises the following steps:
and importing the three-dimensional model file of the porous artificial vertebral body into additive manufacturing equipment, wherein alloy powder is paved in the additive manufacturing equipment in advance, additive manufacturing parameters are set, and the powder material is sintered into the shape of the imported model in a layer-by-layer fusion stacking mode to obtain the porous artificial vertebral body.
In the present invention, the additive manufacturing parameters are preferably the same as those of the powder laser melting method or the powder bed electron beam melting method, and are not described herein again.
After obtaining the porous artificial vertebral body, the invention preferably performs post-treatment on the porous artificial vertebral body, and the post-treatment preferably comprises the following steps:
the obtained porous artificial vertebral body is preferably subjected to powder cleaning, linear cutting, support removal, sand blasting, ultrasonic treatment, cleaning, drying and sterilization in sequence. The present invention does not require any particular procedure, and the procedure can be carried out by a method conventional in the art.
In the invention, when the additive manufacturing method is a powder laser melting method, heat treatment is preferably further included between the ultrasonic treatment and the cleaning, the heat treatment temperature is preferably 900-1100 ℃, and the heat preservation time is preferably 1-2 h.
The porous artificial vertebral body and the method for preparing the same according to the present invention will be described in detail with reference to the following examples, which should not be construed as limiting the scope of the present invention.
Example 1
The porous artificial vertebral body with the structure shown in figures 1-4 is prepared by a powder laser melting method, and the specific operation steps are as follows:
the three-dimensional model file of the porous artificial vertebral body is led into powder bed laser melting additive manufacturing equipment, sufficient medical pure tantalum metal powder is paved in the additive manufacturing equipment in advance, appropriate process parameters (specifically: the layer thickness is 0.03mm, the substrate temperature is 100 ℃, the laser power is 250W, the scanning speed is 200mm/S, and the scanning line interval is 0.09mm) are selected, and the powder material is sintered into the shape of the lead-in model in a layer-by-layer melting and stacking mode.
And taking out the sintered workpiece, and sequentially performing powder cleaning, linear cutting, support removal, sand blasting, ultrasonic cleaning, heat treatment, final cleaning, drying and sterilization.
The obtained porous artificial vertebral body consists of a porous main body structure 1 and a solid reinforcing rib 3, wherein the porous main body structure 1 is provided with six outer surfaces, namely an upper outer surface, a lower outer surface, a left outer surface, a right outer surface, a front outer surface and a rear outer surface, and a bone grafting channel penetrating through the whole vertebral body is arranged between the upper outer surface and the lower outer surface; the front surface of the artificial vertebral body is an arc surface, the back, left and right surfaces are planes, the upper and lower surfaces are wave-shaped, round corners or oblique angle transition is formed between the front, back, left and right surfaces, the upper and lower surfaces are bone fusion surfaces, and the included angle of the surfaces is 7 degrees; the wave surface wave crest (wave trough) distance is 1.5mm, and the depth is 1 mm.
The section of the bone grafting channel is a rounded rectangle, starts from the center of the upper surface and the center of the lower surface, and penetrates through the whole porous main body structure 1. The distance from the edge of the bone grafting passage to the outer surface of the vertebral body is 3 mm.
The center of the front surface is provided with an instrument hole 4 which is communicated with the bone grafting channel. The instrument hole 4 is 6mm in height and 3mm in width.
The solid reinforcing ribs 3 start from the corner points of the outer edge of the vertebral body, end at the corresponding corner points of the bone grafting channel and extend from the upper surface to the lower surface; the thickness of the reinforcing rib is 1.5 mm.
The porosity of the porous body structure 1 was 70%, the average pore diameter was 800 μm, and the average filament diameter was 300. mu.m.
A partial optical micrograph of the porous body structure 1 is shown in fig. 5, and it can be seen from fig. 5 that the porous body structure 1 has a porous structural feature, specifically, a non-regular bionic trabecular porous structure.
Fig. 6 shows a partial SEM image of the porous body structure 1, and fig. 6 (a) and (b) show partial SEM images at different positions. As can be seen from fig. 6, the porous body structure 1 has a rich pore structure.
And testing the compressive strength, the bending strength and the maximum torque of the obtained porous artificial vertebral body according to YS/T1132, YS/T1133 and GB/T101285 standards, wherein the compressive strength, the bending strength and the maximum torque of the obtained porous artificial vertebral body are 33.6MPa, 53MPa and 69N cm.
Performance testing
(1) An equivalent test piece with the porosity of 60% is prepared in the manner of example 1, and the compression strength, the bending strength, the maximum torque and the elastic modulus of the equivalent test piece are tested, and the compression curve, the bending curve, the torsion curve and the compression fatigue curve of the equivalent test piece are shown in fig. 7, 8, 9 and 10 respectively. The multiple curves in each of FIGS. 7-10 represent the results of repeated experiments.
As can be seen from figures 7-10, the porous artificial vertebral body has mechanical properties matched with human bones.
(2) The porous tantalum biomaterial is used for carrying out corresponding cell experiments on NIH-3T3 and HUVEC cells, the growth condition of the cells is observed through a fluorescence microscope, and the proliferation and attachment capacity of the cells on the porous tantalum material is researched.
FIG. 11 is a staining result of co-culture of cells and porous tantalum for 7d, in FIG. 11, green fluorescence is an index of live cells, and red fluorescence is an index of dead cells, and it can be seen from FIG. 11 that the cells are obviously proliferated, the material is basically wrapped, the amount of red fluorescence is very small, and the cell viability is strong.
The porous artificial vertebral body provided by the invention has mechanical properties matched with natural bones and is beneficial to the fusion and ingrowth of bone tissues.
Example 2
A powder electron beam melting method is adopted to prepare the porous artificial vertebral body with the structure shown in the figures 1-4, and the specific operation steps are as follows:
and importing the three-dimensional model file of the porous artificial vertebral body into powder bed electron beam melting additive manufacturing equipment, paving sufficient tantalum-zirconium alloy powder in the additive manufacturing equipment in advance, selecting proper process parameters, and sintering the powder material into a shape imported into the model in a layer-by-layer melting and stacking mode.
The process parameters are as follows:
preheating temperature is 1000 deg.C, current is 10mA, and scanning speed is 1.0 × 105mm/s, and the slice layer thickness is 40 um.
And taking out the sintered workpiece, and sequentially carrying out sand blasting, ultrasonic cleaning, drying and sterilization.
The obtained porous artificial vertebral body consists of a porous main body structure 1 and a solid reinforcing rib 3, wherein the porous main body structure 1 is provided with six outer surfaces, namely an upper outer surface, a lower outer surface, a left outer surface, a right outer surface, a front outer surface and a rear outer surface, and a bone grafting channel penetrating through the whole vertebral body is arranged between the upper outer surface and the lower outer surface; the front surface of the artificial vertebral body is an arc surface, the back, left and right surfaces are planes, the upper and lower surfaces are wave-shaped, round corners or oblique angle transition is formed between the front, back, left and right surfaces, the upper and lower surfaces are bone fusion surfaces, and the included angle of the surfaces is 7 degrees; the wave surface wave crest (wave trough) interval is 1mm, and the depth is 0.8 mm.
The section of the bone grafting channel is a rounded rectangle, starts from the center of the upper surface and the center of the lower surface, and penetrates through the whole porous main body structure 1. The distance from the edge of the bone grafting passage to the outer surface of the vertebral body is 3 mm.
The center of the front surface is provided with an instrument hole 4 which is communicated with the bone grafting channel. The instrument hole 4 is 6mm in height and 3mm in width.
The solid reinforcing ribs 3 start from the corner points of the outer edge of the vertebral body, end at the corresponding corner points of the bone grafting channel and extend from the upper surface to the lower surface; the thickness of the reinforcing rib is 1.5 mm.
The porosity of the porous body structure 1 was 70%, the average pore diameter was 1000 μm, and the average filament diameter was 350 μm.
And testing the compressive strength, the bending strength and the maximum torque of the obtained porous artificial vertebral body according to YS/T1132, YS/T1133 and GB/T101285, wherein the compressive strength, the bending strength and the maximum torque of the obtained porous artificial vertebral body are 51.4MPa, 85.2MPa and 94N cm respectively through testing.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Claims (10)

1. The porous artificial vertebral body is characterized by comprising a porous main body structure (1), wherein the porosity of the porous main body structure (1) is 50-80%; the porous main body structure (1) is provided with an upper surface, a lower surface and four side surfaces, and the upper surface and the lower surface of the porous main body structure (1) are bone connection surfaces; a hollow bone grafting channel (2) is arranged in the porous main body structure (1), and the bone grafting channel penetrates through the upper surface and the lower surface of the porous main body structure (1);
and the solid reinforcing ribs (3) vertically penetrate through the upper surface and the lower surface of the porous main body structure (1).
2. The porous artificial vertebral body of claim 1 wherein the porous artificial vertebral body is made of pure tantalum or tantalum alloy.
3. The porous artificial vertebral body according to claim 1 or 2, wherein the upper and lower surfaces of the porous main body structure (1) form an angle of 0-7 °.
4. The porous artificial vertebral body according to claim 1, characterized in that one side of the porous main body structure (1) is an outer convex arc surface and the remaining three sides are planes; the upper surface and the lower surface of the porous main body structure (1) are wavy surfaces.
5. The porous artificial vertebral body according to claim 4, characterized in that the outer convex arc-shaped surface of the porous main body structure (1) is provided with instrument holes (4), and the instrument holes (4) are communicated with the hollow bone grafting passage (2).
6. The porous artificial vertebral body according to claim 1, characterized in that the number of the solid reinforcing ribs (3) is 4, and the cross section of the 4 solid reinforcing ribs (3) is coincident with the diagonal of the upper and lower surfaces of the porous main body structure (1).
7. The porous artificial vertebral body according to claim 1, characterized in that the pore size of the porous body structure (1) is 300-1500 μm and the filament diameter is 200-350 μm.
8. The method for preparing the porous artificial vertebral body according to any one of claims 1 to 7, comprising the steps of:
preparing the porous artificial vertebral body in an additive manufacturing mode.
9. The method of manufacturing according to claim 8, wherein the additive manufacturing is performed by a powder laser melting method, and parameters of the powder laser melting method include:
the laser power is 150-350W;
the scanning speed is 100-500 mm/s;
the scanning line interval is 0.05-0.15 mm:
the temperature of the substrate is 100-200 ℃;
the thickness of the slicing layer is 0.02-0.05 mm.
10. The method of claim 8, wherein the additive manufacturing process is a powder bed electron beam melting process, and the parameters of the powder bed electron beam melting process include:
the preheating temperature is 750-1500 ℃:
the scanning current is 2-20 mA;
the scanning speed is 1.0 × 104～1.0×106mm/s；
The thickness of the slicing layer is 20-100 μm.
CN202111400620.0A 2021-11-24 2021-11-24 Porous artificial vertebral body and preparation method thereof Pending CN114748218A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202111400620.0A CN114748218A (en) 2021-11-24 2021-11-24 Porous artificial vertebral body and preparation method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202111400620.0A CN114748218A (en) 2021-11-24 2021-11-24 Porous artificial vertebral body and preparation method thereof

Publications (1)

Publication Number Publication Date
CN114748218A true CN114748218A (en) 2022-07-15

Family

ID=82325450

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202111400620.0A Pending CN114748218A (en) 2021-11-24 2021-11-24 Porous artificial vertebral body and preparation method thereof

Country Status (1)

Country Link
CN (1) CN114748218A (en)

Similar Documents

Publication Publication Date Title
EP2296583B1 (en) Dual-sided joint implant having a wear resistant surface and a bioactive surface
JP3935506B2 (en) Diaphysis cortex dwell
CN204971711U (en) High strength aperture footpath metal bone trabecula
CN100588379C (en) Preparation of artificial joint prosthesis with partially controllable porous structure
CN204581484U (en) A kind of 3D with three-dimensional through loose structure prints bone screw
US20110076316A1 (en) Scalable matrix for the in vivo cultivation of bone and cartilage
CN104131195A (en) Preparation method of biomedical porous titanium
JP2003507132A (en) Resorbable bone substitutes and bone constituents
JP2004505677A (en) Porous artificial bone graft and method for producing the same
CN102512267A (en) Bone restoration body with composite porous structure and preparation method thereof
CN104758042A (en) Bone screw of three-dimensional through porous structure
CN101418392A (en) Bio-medical porous titanium products and preparation method thereof
CN104152738A (en) Method for manufacturing biomedical porous nickel titanium alloy
WO2002009597A2 (en) Diaphysial cortical dowel
Tsuchiya et al. Effects of pore size and implant volume of porous hydroxyapatite/collagen (HAp/Col) on bone formation in a rabbit bone defect model
KR20180048678A (en) Large three-dimensional porous skeleton made of active hydroxyapatite obtained by biological morphological transformation of natural structure and its production method
EP2897657B1 (en) Hard scaffold
CN217660301U (en) Porous artificial vertebral body
CN114748218A (en) Porous artificial vertebral body and preparation method thereof
CN113749830A (en) 3D prints porous type interbody fusion cage
CN214511427U (en) 3D prints individualized PEEK material interbody fusion cage with porous structure
RU2632785C1 (en) Hybrid porous structure for osteochondral defects substitution
CN105411725B (en) A kind of bone renovating material preparation method with more-dimensional channels structure
CN210749668U (en) Customized porous tantalum fibula prosthesis
CN211561236U (en) Bone defect repair support

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination