CN114736824B - Agrobacterium rhizogenes for promoting callus of picked potato tubers - Google Patents

Agrobacterium rhizogenes for promoting callus of picked potato tubers Download PDF

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CN114736824B
CN114736824B CN202210377408.5A CN202210377408A CN114736824B CN 114736824 B CN114736824 B CN 114736824B CN 202210377408 A CN202210377408 A CN 202210377408A CN 114736824 B CN114736824 B CN 114736824B
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agrobacterium rhizogenes
potato tubers
callus
potato
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朱艳
陈富
赵元寿
张卫兵
朱建宁
李永才
毕阳
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Gansu Agricultural University
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Abstract

The invention provides agrobacterium rhizogenes Agrobacterium rhizogenes for promoting the callus of picked potato tubers, which is preserved in the China general microbiological culture Collection center (CGMCC) with the preservation number of No:24361. the agrobacterium rhizogenes Agrobacterium rhizogenes strain disclosed by the invention is used for separating self-healing potato tissues, has the advantages of rapid propagation, easiness in collecting strains, easiness in post-harvest treatment of potato tubers, acceleration of the generation speed of a callus surface sealing layer (namely, secondary peripheral skin mainly composed of lignin, polyphenol cork fat and polyester cork fat cell layers) of the potato tubers, and the like, so that the weight loss speed of the potato tubers and the spreading degree of fusarium invasion lesions are reduced.

Description

Agrobacterium rhizogenes for promoting callus of picked potato tubers
Technical Field
The invention belongs to the technical field of microorganism application, and particularly relates to agrobacterium rhizogenes for promoting the callus of post-harvest potato tubers.
Background
Potato (Solanum tuberosum l.) is an important crop for both food and vegetable, tubers are rich in starch, vitamins and minerals, and have high nutritional value, fostering about 10 million people on earth. Over 150 countries and regions can be cultivated in the world, and China is a large country for producing potatoes. With the continuous expansion of potato planting area, potatoes are in an increasingly important position in agricultural production in China. However, potato tubers are fragile and tender in their surface, and are extremely vulnerable to mechanical damage during harvesting and post-harvesting storage, and these wounds tend to dehydrate the tuber tissue and become the main pathway for pathogenic bacteria to invade the tubers, resulting in reduced tuber quality during storage and severe rot.
Although potato tubers naturally heal after mechanical damage to their epidermis, the time required is longer, and the probability of infection of the tubers by germs increases during the longer time of wound self-healing, leading to an increase in post-harvest decay rate. Meanwhile, in commercial production, the potatoes are required to be put into storage as soon as possible after being picked, and places and manual handling required by turnover are reduced, so that the condition of self-healing is lacking after picking, the healing effect is poor, and the risk of rotten cellar in the storage period is increased. The slow self-healing speed of the tubers' epidermis greatly influences the management of the harvesting, storage and transportation of the potato tubers. Although there have been reports of promoting the formation of secondary peripheral skin of wound parts of potato tubers by high temperature or chemical agent treatment, too high a temperature tends to increase the deterioration of physiological metabolism acceleration products of the tubers after harvest, and chemical agent treatment is accompanied by the problem of drug residues. Therefore, the development of biogenic callus promoters has considerable advantages in solving the problem of slow natural callus of tubers in the development of the potato industry.
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides an agrobacterium rhizogenes Agrobacterium rhizogenes strain for promoting the callus of the picked potato tubers.
Agrobacterium rhizogenes is an agrobacterium that infects almost all dicotyledonous plants and a few monocotyledonous plants, resulting in accelerated plant cell growth due to the inducible production of auxins and cytokinins. It is widely found in soil and is commonly used in plant transgenic engineering. The inventors have unexpectedly found that Agrobacterium rhizogenes isolated from potato tuber wounds can accelerate the formation and thickening of secondary pericytes in the callus epidermis closure layer of potato tubers.
The invention provides agrobacterium rhizogenes Agrobacterium rhizogenes for promoting the callus of picked potato tubers, which is preserved in the China general microbiological culture Collection center (CGMCC) with the preservation number of No:24361.
the invention also provides agrobacterium rhizogenes Agrobacterium rhizogenes bacterial liquid for promoting the callus of the picked potato tubers, which is obtained by expanding and culturing the agrobacterium rhizogenes Agrobacterium rhizogenes strain.
Preferably, the preparation method of the bacterial liquid comprises the following steps: agrobacterium rhizogenes Agrobacterium rhizogenes strain was inoculated in the medium and shake cultured at 37.+ -. 1 ℃.
Preferably, the culture medium is: peptone 5g/L, yeast extract 1g/L, naCl 5g/L, pH 7.0.
The invention also provides application of the agrobacterium rhizogenes Agrobacterium rhizogenes strain or agrobacterium rhizogenes Agrobacterium rhizogenes bacterial liquid in preparation of products for promoting the callus of the picked potato tubers.
Preferably, the promotion of post-harvest potato tuber callus is specifically to accelerate the formation and thickening of post-harvest secondary peripheral skin of potato tubers.
The invention also provides a method for promoting the callus of the picked potato tubers, which comprises the steps of spraying the agrobacterium rhizogenes Agrobacterium rhizogenes strain or the agrobacterium rhizogenes Agrobacterium rhizogenes strain liquid on the surface of the damaged or freshly picked potato tubers, and preserving the potato tubers under the condition of room temperature and darkness after airing.
In production practice, the agrobacterium rhizogenes Agrobacterium rhizogenes bacterial liquid is generally selected to be directly sprayed on the surfaces of all fresh potato tubers which are just harvested, and the potato tubers are preserved in the dark at room temperature after being dried. Because the potato harvesting and transportation are all operated in a large batch, compared with the method of picking out damaged potato tubers and independently spraying the damaged potato tubers, the method has the advantage that agrobacterium rhizogenes Agrobacterium rhizogenes bacterial liquid is directly sprayed on the surfaces of all fresh potato tubers, so that the treatment efficiency is higher.
The invention also provides a biological source healing promoting agent for potatoes, and the active ingredients of the biological source healing promoting agent comprise the agrobacterium rhizogenes Agrobacterium rhizogenes strain or agrobacterium rhizogenes Agrobacterium rhizogenes bacterial liquid.
The agrobacterium rhizogenes Agrobacterium rhizogenes strain disclosed by the invention is used for separating self-healing potato tissues, has the advantages of rapid propagation, easiness in collecting strains, easiness in post-harvest treatment of potato tubers, acceleration of the generation speed of a callus surface sealing layer (namely, secondary peripheral skin mainly composed of lignin, polyphenol cork fat and polyester cork fat cell layers) of the potato tubers, and the like, so that the weight loss speed of the potato tubers (see figure 2) and the sickle cell invasion disease spot expansion degree (see figure 3) are reduced.
The invention carries out spray treatment of agrobacterium rhizogenes Agrobacterium rhizogenes bacterial suspension within 24 hours after potato tuber harvest: washing the picked potato tubers, soaking and sterilizing the potato tubers by sodium hypochlorite, washing the potato tubers by deionized water, and airing the potato tubers; uniformly spraying the target bacterial suspension on the surface of artificially damaged potato tubers, naturally airing, and then filling the potato tubers into a perforated fresh-keeping bag, and curing the potato tubers at room temperature under dark conditions. The agrobacterium rhizogenes Agrobacterium rhizogenes used in the invention is the epidermis which is separated from the natural healing of damaged potato tubers, is not genetically modified, is identified to belong to wild agrobacterium rhizogenes, and can promote the formation of secondary peripheral skin after the potato tubers are picked and accelerate the healing process. The invention can solve the problem of slow healing speed caused by long time required for natural repair of the skin wound after potato tuber harvest.
Drawings
The accompanying drawings are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate the invention and together with the embodiments of the invention, serve to explain the invention. In the drawings:
FIG. 1 is a colony morphology of Agrobacterium rhizogenes strains of the present invention.
FIG. 2 is a graph showing the effect of the strain of the invention on the weight loss rate during potato tuber callus.
FIG. 3 shows the effect of the strain of the invention on the diameter of lesions caused by F.sambucus during potato tuber callus. A is the diameter of the lesion after 7 days of tuber inoculation; b is the diameter of the lesion after 14 days of tuber inoculation of the callus; c is the inhibition rate of the lesion diameter.
FIG. 4 shows the effect of the strain of the invention on the deposition of polyphenol cork fat (SPP) during potato tuber callus (panel A) and the number of cell layers (panel B).
FIG. 5 shows the effect of the strain of the invention on the deposition of polyester cork fat (SPA) (Panel A) and the number of cell layers (Panel B) during potato tuber callus.
Detailed Description
The following examples facilitate a better understanding of the present invention, but are not intended to limit the same. The experimental methods in the following examples are conventional methods unless otherwise specified. The test materials used in the examples described below are commercially available unless otherwise specified.
Example 1
The agrobacterium rhizogenes strain is obtained by screening from damaged tuber epidermis healing tissues stored in a ventilation warehouse of potatoes in Qingzhen county, gansu province, is classified and named agrobacterium rhizogenes Agrobacterium rhizogenes, and is preserved in China general microbiological culture Collection center (CGMCC) on 1 month 21 of 2022, wherein the preservation unit address is North Chen West Lu 1 No. 3 in the Qing region of Beijing city, and the preservation number is CGMCC No:24361.
the screening method of agrobacterium rhizogenes Agrobacterium rhizogenes comprises the following steps: collecting tubers stored in a ventilating warehouse of a town of Jungshi, gansu province, and Weiyuan county, selecting tubers with damaged epidermis and naturally healed, placing into a fresh-keeping bag, transporting to a laboratory at low temperature of 4deg.C, cleaning with tap water, sterilizing with sodium hypochlorite, naturally airing, removing damaged epidermis with a blade under aseptic condition, taking tuber tissue of 1-2 mm under skin, and cutting into pieces of about 1mm 2 Weighing 1g of small pieces, diluting to 10 in 9mL of sterile water -3 ～10 -7 1mL of cells/mL is absorbed and coated in LB culture medium, the culture is carried out at the constant temperature of 37 ℃, single colony is selected for further streak separation, molecular biological identification is carried out until pure strain is obtained after separation, and one strain is agrobacterium rhizogenes.
Wherein, the composition of LB culture medium: 10g/L of tryptone (peptone), 5g/L of yeast extract (yeast powder), 5g/L of NaCl and pH 7.0. Sterilizing at 121deg.C for 25 min.
The agrobacterium rhizogenes Agrobacterium rhizogenes of the invention have the following biological properties:
1. morphological characteristics: the gram staining result of the strain is negative, rod-shaped, no spore and capsular.
2. Culturing characteristics: the strain is cultivated on LB culture medium plate at 37 ℃ for 24 hours, and the bacterial colony is pale yellow, round, raised and smooth in surface.
3. Genetic characterization (strain 16srRNA sequence): the invention detects the 16srRNA sequence of the strain, as follows, the full length is 1418bp.
ACGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCGCCCCGCAAGGGGAGCGGCAGACGGGTGAGTAACACATGGGAATCTACCCTTTTCTACGGAATAACGCAGGGAAACTTGTGCTAATACCGTATGTGTCCTTCGGGAGAAAGATTTATCGGGAAAGGATGAAGCATGAGCCCGCGTTGGATTAGCTAGTTGGTGGGGTAAAGGCCTACCAAGGCGACGATCCATAGCTGGTCTGAGAGGATGATCAGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATGCCGCGTGAGTGATGAAGGCCCTAGGGTTGTAAAGCTCTTTCAAGCCTGATCCAGCCATGCCGCGTGAGTGATGAAGGCCCTAGGGTTGTAAAGCTCTTTCACCGGAGAAGATAATGACGGTATCCGGAGAAGAAGCCCCGGCTAACTTCGTGCCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTAGGCGGATCGATCAGTCAGGGGTGAAATCCCAGGGCTCAACCCTGGAACTGCCTTTGATACTGTCGATCTGGAGTATGGAAGAGGTGAGTGGAATTCCGAGTGTAGAGGTGAAATTCGTAGATATTCGGAGGAACACCAGTGGCGAAGCGGCTCACTGGTCCATTACTGACGCTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAATGTTAGCCGTCGGGCAGTATACTGTTCGGTGGCGCACGTAACGCATTAAACATTCCGCCTGGGGAGTACGGTCGCAAGATTAAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGCAGAACCTTACCAGCCCTTGACATCCTGTGTTACCCGTAGAGATATGGGGTCCACTTCGGTGGCGCAGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCGCCCTTAGTTGCCAGCATTCAGTTGGGCACTCTAAGGGGACTGCCGGTGATAAGCCGAGAGGAAGGTGGGGATGACGTCAAGTCCTCATGGCCCTTACGGGCTGGGCTACACACGTGCTACAATGGTGGTGACAGGGGCAGCGAGCACGCGAGTGTGAGCTAATCTCCAAAAGCCATCTCAGTTCGGATTGCACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTTGGTTTTACCCGAAGGTAGTGCG。
Example 2 agrobacterium rhizogenes Agrobacterium rhizogenes CGMCC No of the invention: 24361 Performance test for promoting callus of Potato tubers
1. Agrobacterium rhizogenes Agrobacterium rhizogenes CGMCC No:24361 promoting potato tuber callus
Agrobacterium rhizogenes Agrobacterium rhizogenes CGMCC No: preparation of 24361 bacterial suspension: filling 100mL of seed culture medium into 250mL triangular flask, inoculating one ring of Agrobacterium rhizogenes Agrobacterium rhizogenes, shaking and culturing at 37deg.C and shaking table rotation speed of 180r/min for 24 hr to obtain seed solution, centrifuging at 4deg.C and 8000r/min for 10min to obtain precipitate as thallus, re-suspending the collected thallus with sterile water to obtain 10 7 The bacterial suspension with the concentration of cells/mL is sprayed by a sprayer.
Wherein, the seed culture medium comprises: peptone 5g/L, yeast extract 1g/L, naCl 5g/L, pH 7.0.
A sprayer: the particles of the atomized droplets are about 5 μm to 15 μm by an electric atomizer or a manual atomizer.
The potato tuber treatment method comprises the following steps: adopting an Atlantic potato variety (or a Gansu potato variety), washing potato tubers with tap water, soaking and sterilizing with sodium hypochlorite with the volume concentration of 0.1% for 10min, washing with sterile water, and air-drying; the skin scraping knife is used for carrying out artificial injury on the superficial epidermis of the tuber, and the wound formed after the picking is simulated. The production suggests that the treatment be carried out within 24 hours after tuber harvest. This experiment was performed with a spray treatment immediately after tuber damage. The prepared agrobacterium rhizogenes Agrobacterium rhizogenes CGMCC No were applied to the experimental group using a sterile nebulizer: 24361 the bacterial suspension is uniformly sprayed on the wound surface of potato tuber, and the potato tuber is put into a perforated fresh-keeping box after natural air drying, and is subjected to callus under the condition of darkness at room temperature. Sterile water spray treatment without added bacterial suspension was used as a control treatment.
2. Determination of the weight loss rate during potato tuber callus:
the weight loss rate of potato tubers in storage was determined by differential methods. And (3) placing the potato tubers subjected to the spraying treatment with sterile water and bacterial liquid under the condition of darkness at room temperature, measuring the weight of the potato tubers after 3 days, 5 days, 7 days and 14 days of curing, and calculating the weight loss rate in the curing process according to the following formula. The test was repeated 3 times.
Calculation of the weight loss ratio:
weight loss (%) = [ (W) 1 -W 2 )/W 1 ]×100
W in the formula 1 Weight (g) of pre-callus tubers;
W 2 weight of tubers after callus (g).
The measured time of the post-callus measurement point of the potato tubers was significantly lower for the Agrobacterium rhizogenes treated potato tubers than for the sterile water treated control. From about day 3 after callus to 14 after callus, the Agrobacterium rhizogenes treated potato tuber weight loss rate was about 32% -52% lower than the control potato tuber weight loss rate.
3. Determination of plaque diameter during potato tuber callus:
inoculating spore suspension of Fusarium sambucinum (Fusarium sambucinum) at concentration of 10 at potato tuber callus 7d and 14d respectively 7 cells/mL, then placing the potato tubers into a fresh-keeping box for preservation at a dark room temperature, and measuring the diameter of colonies of damaged parts of the potato tubers by a crisscross method at fixed points every day after inoculation. After the potato tubers are subjected to the callus of 7d and 14d, compared with a control, the agrobacterium rhizogenes treatment reduces the infection speed of the potato tubers and the fusarium sambucus, and the diameter of the disease spots is obviously lower than that of the control treatment group.
Calculation of lesion diameter inhibition rate:
lesion diameter inhibition rate (%) = [ (D) Ck -D At )/D ck ]×100
D in Ck Control group lesion diameter (mm) for sterile water treatment;
D At group plaque diameter (mm) was treated for agrobacterium rhizogenes.
After the potato tuber is subjected to 7d callus, compared with a control, the inhibition rate of the potato tuber treated by agrobacterium rhizogenes on the diameter of a disease spot caused by fusarium infection of elder can reach 34%; after 14d of callus, the inhibition rate of the potato tuber treated by agrobacterium rhizogenes on the diameter of the lesion caused by fusarium sambucinum infection can reach 37.5% compared with a control.
4. Staining observation of the polyphenol cork fat SPP in the peripheral skin of the damaged tuber of the potato and measurement of the cell layer thickness:
potato tubers of 3d, 5d, 9d and 12d of the calli were taken respectively, and wound tissues were cut into slices (length and width were about 1cm, and thickness was 0.2 to 0.3 mm) perpendicular to the wound surface with stainless steel blades. Washing 6-8 times with sterile water to wash out starch, dyeing for 45min with 0.1% berberine, washing 2-3 times with sterile water, washing 2-3 times with 75% ethanol, washing 1-2 times with 95% ethanol, removing dye, counterstaining for 1.5min with 0.25% toluidine blue, finally washing with sterile water and 75% ethanol to remove dye, dyeing SPP, and taking pictures under a microscope by fluorescent observation. SPP cell layer thickness measurements were performed using IS Capture image software.
After being measured, the agrobacterium rhizogenes treatment, the SPP of the damaged epidermis of the potato tuber is generated at a higher speed than that of a control of sterile water treatment, and the thickness of the cell layer shows a statistically significant difference from the 9d after the callus.
5. Staining observation of polyester cork fat SPA in potato damaged tuber surface peripheral skin and measurement of cell layer thickness:
potato tubers of 3d, 5d, 9d and 12d of the calli were taken respectively, and wound tissues were cut into slices (length and width were about 1cm, and thickness was 0.2 to 0.3 mm) perpendicular to the wound surface with stainless steel blades. Washing with sterile water for 6-8 times to wash out starch, dyeing with 0.05% toluidine blue for 45min, washing with sterile water for 2-3 times, washing with 75% ethanol for 2-3 times, washing with 95% ethanol for 1-2 times, and removing the dye. The SPA can be stained by counterstaining with 1% neutral red and finally washing the dye with sterile water and 75% ethanol, and photographed under a fluorescent microscope. The thickness of SPA cell layer was measured using IS Capture image software.
After being measured, the agrobacterium rhizogenes treatment, the potato tuber injured epidermis SPA has a higher generation speed than that of a sterile water treatment control, and the cell layer thickness shows a statistically significant difference from the 3 rd day after the callus.
The accumulation of SPP and SPA can be used for concluding that the generation speed of the peripheral skin on the surface of the potato tuber can be increased by 3-5 days after the potato tuber is treated by agrobacterium rhizogenes, and the callus of the potato tuber is promoted.
Finally, it should be noted that: the foregoing description is only a preferred embodiment of the present invention, and the present invention is not limited thereto, but it is to be understood that modifications and equivalents of some of the technical features described in the foregoing embodiments may be made by those skilled in the art, although the present invention has been described in detail with reference to the foregoing embodiments. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Sequence listing
<110> Gansu agricultural university
<120> A set of Agrobacterium rhizogenes promoting callus on post-harvest potato tubers
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1418
<212> DNA
<213> Agrobacterium rhizogenes (Agrobacterium rhizogenes)
<400> 1
acgaacgctg gcggcaggct taacacatgc aagtcgagcg ccccgcaagg ggagcggcag 60
acgggtgagt aacacatggg aatctaccct tttctacgga ataacgcagg gaaacttgtg 120
ctaataccgt atgtgtcctt cgggagaaag atttatcggg aaaggatgaa gcatgagccc 180
gcgttggatt agctagttgg tggggtaaag gcctaccaag gcgacgatcc atagctggtc 240
tgagaggatg atcagccaca ttgggactga gacacggccc aaactcctac gggaggcagc 300
agtggggaat attggacaat gggcgcaagc ctgatccagc catgccgcgt gagtgatgaa 360
ggccctaggg ttgtaaagct ctttcaagcc tgatccagcc atgccgcgtg agtgatgaag 420
gccctagggt tgtaaagctc tttcaccgga gaagataatg acggtatccg gagaagaagc 480
cccggctaac ttcgtgccag cagccgcggt aatacgaagg gggctagcgt tgttcggaat 540
tactgggcgt aaagcgcacg taggcggatc gatcagtcag gggtgaaatc ccagggctca 600
accctggaac tgcctttgat actgtcgatc tggagtatgg aagaggtgag tggaattccg 660
agtgtagagg tgaaattcgt agatattcgg aggaacacca gtggcgaagc ggctcactgg 720
tccattactg acgctgaggt gcgaaagcgt ggggagcaaa caggattaga taccctggta 780
gtccacgccg taaacgatga atgttagccg tcgggcagta tactgttcgg tggcgcacgt 840
aacgcattaa acattccgcc tggggagtac ggtcgcaaga ttaaaactca aaggaattga 900
cgggggcccg cacaagcggt ggagcatgtg gtttaattcg aagcaacgcg cagaacctta 960
ccagcccttg acatcctgtg ttacccgtag agatatgggg tccacttcgg tggcgcagag 1020
acaggtgctg catggctgtc gtcagctcgt gtcgtgagat gttgggttaa gtcccgcaac 1080
gagcgcaacc ctcgccctta gttgccagca ttcagttggg cactctaagg ggactgccgg 1140
tgataagccg agaggaaggt ggggatgacg tcaagtcctc atggccctta cgggctgggc 1200
tacacacgtg ctacaatggt ggtgacaggg gcagcgagca cgcgagtgtg agctaatctc 1260
caaaagccat ctcagttcgg attgcactct gcaactcgag tgcatgaagt tggaatcgct 1320
agtaatcgcg gatcagcatg ccgcggtgaa tacgttcccg ggccttgtac acaccgcccg 1380
tcacaccatg ggagttggtt ttacccgaag gtagtgcg 1418

Claims (8)

1. An agrobacterium rhizogenes Agrobacterium rhizogenes for promoting callus of post-harvest potato tubers, characterized in that: the microbial strain is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC No:24361.
2. the agrobacterium rhizogenes Agrobacterium rhizogenes bacterial liquid for promoting the callus of the picked potato tubers is characterized in that: is obtained by culturing the Agrobacterium rhizogenes Agrobacterium rhizogenes strain according to claim 1 in an expanded manner.
3. The agrobacterium rhizogenes Agrobacterium rhizogenes bacterial liquid according to claim 2, wherein the bacterial liquid is characterized by: the preparation method of the bacterial liquid comprises the following steps: agrobacterium rhizogenes Agrobacterium rhizogenes strain was inoculated in the medium and shake cultured at 37.+ -. 1 ℃.
4. An agrobacterium rhizogenes Agrobacterium rhizogenes bacterial liquid according to claim 3, wherein: the culture medium is as follows: peptone 5g/L, yeast extract 1g/L, naCl 5g/L, pH 7.0.
5. Use of the agrobacterium rhizogenes Agrobacterium rhizogenes strain of claim 1 or the agrobacterium rhizogenes Agrobacterium rhizogenes bacterial liquid of claim 2 for the preparation of a product for promoting the healing of post-harvest potato tubers.
6. The use according to claim 5, characterized in that: the promotion of the callus of the picked potato tuber is specifically to accelerate the formation and thickening of the secondary peripheral skin of the picked potato tuber.
7. A method for promoting callus of post-harvest potato tubers, comprising: the agrobacterium rhizogenes Agrobacterium rhizogenes strain of claim 1 or the agrobacterium rhizogenes Agrobacterium rhizogenes bacterial liquid of claim 2 is sprayed on the surface of damaged or freshly harvested fresh potato tubers, and the potato tubers are preserved in dark at room temperature after being dried.
8. A biogenic callus promoter for potatoes, the active ingredients of which comprise the agrobacterium rhizogenes Agrobacterium rhizogenes strain of claim 1 or the agrobacterium rhizogenes Agrobacterium rhizogenes bacterial liquid of claim 2.
CN202210377408.5A 2022-04-12 2022-04-12 Agrobacterium rhizogenes for promoting callus of picked potato tubers Active CN114736824B (en)

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CN110122561A (en) * 2019-06-04 2019-08-16 甘肃农业大学 Olive Candida promotes to adopt the callus method of rear potato tubers

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CN110122561A (en) * 2019-06-04 2019-08-16 甘肃农业大学 Olive Candida promotes to adopt the callus method of rear potato tubers

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