CN114736823B - Agrobacterium tumefaciens for promoting callus of picked potato tubers - Google Patents
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Abstract
The invention provides an agrobacterium tumefaciens Agrobacterium tumefaciens for promoting the callus of picked potato tubers, which is preserved in the China general microbiological culture Collection center (CGMCC) with the preservation number of No:24362. the agrobacterium tumefaciens Agrobacterium tumefaciens strain related by the invention is used for separating self-healing potato tissues, has the characteristics of rapid propagation, easiness in collection of strains, easiness in treatment of picked tubers and acceleration of the generation speed of a tuber callus epidermis sealing layer (namely, secondary pericycle which mainly consists of lignin, polyphenol cork fat and polyester cork fat cell layers), so that the weight loss speed of tubers and the spreading of fusarium invasion lesions are reduced.
Description
Technical Field
The invention belongs to the technical field of microorganism application, and particularly relates to agrobacterium tumefaciens for promoting callus of post-harvest potato tubers.
Background
Potato (Solanum tuberosum l.) is an important crop for both food and vegetable, tubers are rich in starch, vitamins and minerals, and have high nutritional value, fostering about 10 million people on earth. Over 150 countries and regions are available worldwide for potato cultivation. China is a large country for producing potatoes, and along with the continuous expansion of potato planting areas, potatoes have an increasingly important position in agricultural production in China. However, potato tubers are fragile and tender in their surface, and are extremely vulnerable to mechanical damage during harvesting and post-harvesting storage, and these wounds tend to dehydrate the tuber tissue and become the main pathway for pathogenic bacteria to invade the tubers, resulting in reduced tuber quality during storage and severe rot.
Although potato tubers naturally heal after mechanical damage to their epidermis, the time required is longer, and the probability of infection of the tubers by germs increases during the longer time of wound self-healing, leading to an increase in post-harvest decay rate. Meanwhile, in commercial production, the potatoes are required to be put into storage as soon as possible after being picked, and places and manual handling required by turnover are reduced, so that the condition of self-healing is lacking after picking, the healing effect is poor, and the risk of rotten cellar in the storage period is increased. The slow self-healing speed of the tubers' epidermis greatly influences the management of the harvesting, storage and transportation of the potato tubers. Although there have been reports of promoting the formation of secondary peripheral skin of the wound site of potato tubers by treatment with high temperature or chemical agents, excessive temperature tends to increase the deterioration of physiological metabolism acceleration products of the tubers after harvest, and chemical drug treatment is accompanied by the problem of drug residue. Therefore, the development of biogenic callus promoters has considerable advantages in solving the problem of slow natural callus of tubers in the development of the potato industry.
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides an agrobacterium tumefaciens Agrobacterium tumefaciens strain for promoting the callus speed of the picked potato tubers.
Agrobacterium tumefaciens is a dicotyledonous plant that can be integrated into plant tissue by T-DNA transfer and induced to produce auxin and cytokinin, resulting in increased cell growth. It is widely found in soil, and only a few are pathogenic and are generally applied to plant transgenic engineering, but the inventor of the application unexpectedly finds that agrobacterium tumefaciens separated from potato tuber wounds can accelerate the formation and thickening of secondary peripheral skin of potato tubers after harvest.
The invention provides an agrobacterium tumefaciens Agrobacterium tumefaciens for promoting the callus of picked potato tubers, which is preserved in the China general microbiological culture Collection center (CGMCC) with the preservation number of No:24362.
the invention also provides an agrobacterium tumefaciens Agrobacterium tumefaciens bacterial liquid for promoting the callus of the picked potato tubers, which is obtained by expanding and culturing the agrobacterium tumefaciens Agrobacterium tumefaciens strain.
Preferably, the preparation method of the bacterial liquid comprises the following steps: agrobacterium tumefaciens Agrobacterium tumefaciens strain is inoculated in a culture medium and shake-cultured at 37+ -1deg.C.
Preferably, the culture medium is: peptone 5g/L, yeast extract 1g/L, naCl 5g/L, pH 7.0.
The invention also provides application of the agrobacterium tumefaciens Agrobacterium tumefaciens strain or the agrobacterium tumefaciens Agrobacterium tumefaciens bacterial liquid in preparation of products for promoting the callus of the picked potato tubers.
Preferably, the promotion of post-harvest potato tuber callus is specifically to accelerate the formation and thickening of post-harvest secondary peripheral skin of potato tubers.
The invention also provides a method for promoting the callus of the picked potato tubers, which comprises the steps of spraying the agrobacterium tumefaciens Agrobacterium tumefaciens strain or the agrobacterium tumefaciens Agrobacterium tumefaciens bacterial liquid on the surface of the damaged or freshly picked potato tubers, airing the potato tubers and preserving the potato tubers under the condition of room temperature and darkness.
In production practice, the agrobacterium tumefaciens Agrobacterium tumefaciens bacterial liquid is generally selected to be directly sprayed on the surfaces of all fresh potato tubers which are just harvested, and the potato tubers are preserved in the dark at room temperature after being dried. Because the potato harvesting and transportation are all operated in a large scale, compared with the method of picking out damaged potato tubers and independently spraying the damaged potato tubers, the method has the advantage that the agrobacterium tumefaciens Agrobacterium tumefaciens bacterial liquid is directly sprayed on the surfaces of all fresh potato tubers, so that the treatment efficiency is higher.
The invention also provides a biological source healing promoting agent for potatoes, and the active ingredients of the biological source healing promoting agent comprise the agrobacterium tumefaciens Agrobacterium tumefaciens strain or the agrobacterium tumefaciens Agrobacterium tumefaciens bacterial liquid.
The agrobacterium tumefaciens Agrobacterium tumefaciens strain related by the invention is used for separating self-healing potato tissues, has the advantages of rapid propagation, easy collection of strains, easy treatment of the picked tubers, and acceleration of the generation speed of a tuber callus epidermis sealing layer (namely, secondary pericycle mainly consisting of lignin, polyphenol cork fat and polyester cork fat cell layers), thereby reducing the weight loss speed of the tubers (see figure 2) and the spreading degree of fusarium invasion lesions (see figure 3).
The invention carries out spray treatment of the agrobacterium tumefaciens Agrobacterium tumefaciens bacterial suspension within 24 hours after potato tuber harvest: washing the picked potato tubers, soaking and sterilizing the potato tubers by sodium hypochlorite, washing the potato tubers by deionized water, and airing the potato tubers; uniformly spraying the target bacterial suspension on the surface of artificially damaged potato tubers, naturally airing, and then filling the potato tubers into a perforated fresh-keeping bag, and curing the potato tubers at room temperature under dark conditions. The agrobacterium tumefaciens Agrobacterium tumefaciens used in the invention is the epidermis which is separated from the natural healing of damaged potato tubers, is not genetically modified, is identified to belong to wild agrobacterium tumefaciens, and can promote the formation of secondary peripheral skin after the potato tubers are picked and accelerate the healing process. The invention can solve the problem of slow healing speed caused by long time required for natural repair of the skin wound after potato tuber harvest.
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The accompanying drawings are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate the invention and together with the embodiments of the invention, serve to explain the invention. In the drawings:
FIG. 1 is a colony morphology of Agrobacterium tumefaciens strains of the present invention.
FIG. 2 is a graph showing the effect of the strain of the invention on the weight loss rate during potato tuber callus.
FIG. 3 shows the effect of the strain of the invention on the diameter of lesions caused by F.sambucus during potato tuber callus. A is the diameter of the lesion after 7 days of tuber inoculation; b is the diameter of the lesion after 14 days of tuber inoculation of the callus; c is the inhibition rate of the lesion diameter.
FIG. 4 shows the effect of the strain of the invention on the deposition of polyphenol cork fat (SPP) during potato tuber callus (panel A) and the number of cell layers (panel B).
FIG. 5 shows the effect of the strain of the invention on the deposition of polyester cork fat (SPA) (Panel A) and the number of cell layers (Panel B) during potato tuber callus.
Detailed Description
The following examples facilitate a better understanding of the present invention, but are not intended to limit the same. The experimental methods in the following examples are conventional methods unless otherwise specified. The test materials used in the examples described below, unless otherwise specified, were purchased from conventional Biochemical reagents. The quantitative tests in the following examples were all set up in triplicate and the results averaged.
Example 1
The agrobacterium tumefaciens strain is obtained by screening damaged tuber epidermis stored in a ventilation warehouse of potatoes in Qing Zhen Chuan of Qing county, gansu province, and is classified as agrobacterium tumefaciens Agrobacterium tumefaciens which is preserved in China general microbiological culture Collection center (CGMCC) on 1 month 21 of 2022, wherein the preserving unit address is North Chen West Lu No. 1 of the Guangyang area of Beijing, and the preserving number is CGMCC No:24362.
the screening method of the agrobacterium tumefaciens Agrobacterium tumefaciens comprises the following steps: collecting tubers stored in a ventilating warehouse of a town of Jungshi, gansu province, and Weiyuan county, selecting tubers with damaged epidermis and naturally healed, placing into a fresh-keeping bag, transporting to a laboratory at low temperature of 4deg.C, cleaning with tap water, sterilizing with sodium hypochlorite, naturally airing, removing damaged epidermis with a blade under aseptic condition, taking tuber tissue of 1-2 mm under skin, and cutting into pieces of about 1mm 2 Weighing 1g of small pieces, diluting to 10 in 9mL of sterile water -3 ～10 -7 1mL of the cells/mL is absorbed and coated in LB culture medium, the culture is carried out at the constant temperature of 37 ℃, and single colony is selected for streak separationMolecular biological identification is carried out until pure strains are obtained by separation, wherein one strain is agrobacterium tumefaciens.
Wherein, the composition of LB culture medium: 10g/L of tryptone (peptone), 5g/L of yeast extract (yeast powder), 5g/L of NaCl and pH 7.0. Sterilizing at 121deg.C for 25 min.
The agrobacterium tumefaciens Agrobacterium tumefaciens of the present invention has the following biological characteristics:
1. morphological characteristics: the gram staining result of the strain is negative, rod-shaped, no spore and capsular.
2. Culturing characteristics: the strain is cultivated on LB culture medium plate at 37 ℃ for 24 hours, and the bacterial colony is pale yellow, round, convex, smooth in surface and neat in edge.
3. Genetic characterization (strain 16srRNA sequence): the invention detects the 16srRNA sequence of the strain, as follows, the full length is 1406bp.
GGTTAGCTGCCTCCTTGCGGTTAGCGCACTACCTTCGGGTAAAACCAACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCAGCATGCTGATCTGCGATTACTAGCGATTCCAACTTCATGCACTCGAGTTGCAGAGTGCAATCCGAACTGAGATGGCTTTTGGAGATTAGCTCGACATCGCTGTCTCGCTGCCCACTGTCACCACCATTGTAGCACGTGTGTAGCCCAGCCCGTAAGGGCCATGAGGACTTGACGTCATCCCCACCTTCCTCTCGGCTTATCACCGGCAGTCCCCTTAGAGTGCCCAACTAAATGCTGGCAACTAAGGGCGAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTTCTGGGGCCAGCCTAACTGAAGGACAATGTCTCCACTGCCCAAACCCCGAATGTCAAGAGCTGGTAAGGTTCTGCGCGTTGCTTCGAATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTTAATCTTGCGACCGTACTCCCCAGGCGGAATGTTTAATGCGTTAGCTGCGCCACCGAACAGTATACTGCCCGACGGCTAACATTCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTAATGGACCAGTAAGCCGCCTTCGCCACTGGTGTTCCTCCGAATATCTACGAATTTCACCTCTACACTCGGAATTCCACTTACCTCTTCCATACTCAAGATACCCAGTATCAAAGGCAGTTCCAGAGTTGAGCTCTGGGATTTCACCCCTGACTTAAATATCCGCCTACGTGCGCTTTACGCCCAGTAATTCCGAACAACGCTAGCCCCCTTCGTATTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTCCGGATACCGTCATTATCTTCTCCGGTGAAAGAGCTTTACAACCCTAAGGCCTTCATCACTCACGCGGCATGGCTGGATCAGGCTTGCGCCCATTGTCCAATATTCCCCACTGCTGCCTCCCGTAGGAGTTTGGGCCGTGTCTCAGTCCCAATGTGGCTGATCATCCTCTCAGACCAGCTATGGATCGTCGCCTTGGTAGGCCTTTACCCCACCAACTAGCTAATCCAACGCGGGCCAATCCTTCCCCGATAAATCTTTCCCCCGTAGGGCGTATGCGGTATTAATTCCAGTTTCCCGGAGCTATTCCGCAGGAAAGGGTATGTTCCCACGCGTTACTCACCCGTCTGCCACTCCCCTTGCGGGGCGTTCGACTTGCATGTGTTAAGCCTGCCCGCCAGCGTTCGTTCTGAGCCAGGATTCAAACTCTA。
Example 2 agrobacterium tumefaciens Agrobacterium tumefaciens CGMCC No of the present invention: 24362 Performance test for promoting callus of Potato tubers
1. Agrobacterium tumefaciens Agrobacterium tumefaciens CGMCC No:24362 promoting potato tuber callus
Agrobacterium tumefaciens Agrobacterium tumefaciens CGMCC No: preparation of 24362 bacterial suspension: filling 100mL of seed culture medium into 250mL triangular flask, inoculating one ring of Agrobacterium tumefaciens Agrobacterium tumefaciens, shaking culture at 37deg.C and shaking table rotation speed of 180r/min for 24 hr to obtain seed solution, centrifuging at 4deg.C and 8000r/min for 10min to obtain precipitate as thallus, re-suspending the collected thallus with sterile water to obtain 10 7 The bacterial suspension with the concentration of cells/mL is sprayed by a sprayer.
Wherein, the seed culture medium comprises: peptone 5g/L, yeast extract 1g/L, naCl 5g/L, pH 7.0.
A sprayer: the particles of the atomized droplets are about 5 μm to 15 μm by an electric atomizer or a manual atomizer.
The potato tuber treatment method comprises the following steps: adopting an Atlantic potato variety (or a Gansu potato variety), washing potato tubers with tap water, soaking and sterilizing with sodium hypochlorite with the volume concentration of 0.1% for 10min, washing with sterile water, and air-drying; the skin scraping knife is used for carrying out artificial injury on the superficial epidermis of the tuber, and the wound formed after the picking is simulated. The production suggests that the treatment be carried out within 24 hours after tuber harvest. This experiment was performed with a spray treatment immediately after tuber damage. The prepared agrobacterium tumefaciens Agrobacterium tumefaciens CGMCC No was sprayed with a sterile sprayer for the experimental group: 24362 the bacterial suspension is uniformly sprayed on the wound surface of potato tuber, and the potato tuber is put into a perforated fresh-keeping box after natural air drying, and is subjected to callus under the condition of darkness at room temperature. Sterile water spray treatment without added bacterial suspension was used as a control treatment.
2. Determination of the weight loss rate during potato tuber callus:
the weight loss rate of potato tubers in storage was determined by differential methods. And (3) placing the potato tubers subjected to the spraying treatment with sterile water and bacterial liquid under the dark condition at room temperature, measuring the weight of the tubers after 3 days, 5 days, 7 days and 14 days of callus, and calculating the weight loss rate in the callus process according to the following formula. The test was repeated 3 times.
Calculation of the weight loss ratio:
weight loss (%) = [ (W) 1 -W 2 )/W 1 ]×100
W in the formula 1 Weight (g) of pre-callus tubers;
W 2 weight of tubers after callus (g).
The measured time of the post-callus measurement point of the potato tubers was significantly lower in the Agrobacterium tumefaciens-treated potato tubers than in the sterile water-treated control group. From about day 3 after callus to 14 after callus, the weight loss rate of the tubers of the Agrobacterium tumefaciens-treated group was about 32% -49% lower than that of the tubers of the control group.
3. Determination of plaque diameter during potato tuber callus:
inoculating spore suspension of Fusarium sambucinum (Fusarium sambucinum) at concentration of 10 at potato tuber callus 7d and 14d respectively 7 cells/mL, then placing the potato tubers into a fresh-keeping box for preservation at a dark room temperature, and measuring the diameter of colonies of damaged parts of the potato tubers by a crisscross method at fixed points every day after inoculation. After the potato tubers are subjected to the callus of 7d and 14d, compared with a control, the agrobacterium tumefaciens treatment reduces the infection speed of the fusarium sambucus of the potato tubers, and the diameter of the disease spots is obviously lower than that of the control treatment group.
Calculation of lesion diameter inhibition rate:
lesion diameter inhibition rate (%) = [ (D) Ck -D At )/D ck ]×100
D in Ck Control group lesion diameter (mm) for sterile water treatment;
D At group plaque diameter (mm) was treated for agrobacterium tumefaciens.
After the potato tubers are subjected to 7d callus, compared with a control, the inhibition rate of the tubers treated by the agrobacterium tumefaciens on the diameter of the lesion caused by the infection of the fusarium sambucus can reach up to 35%; after 14d of callus, the inhibition rate of the potato tuber treated by the agrobacterium tumefaciens on the diameter of the lesion caused by the infection of fusarium sambucus can reach 31 percent compared with a control.
4. Staining observation of the polyphenol cork fat SPP in the peripheral skin of the damaged tuber of the potato and measurement of the cell layer thickness:
potato tubers of 3d, 5d, 9d and 12d of the calli were taken respectively, and wound tissues were cut into slices (length and width were about 1cm, and thickness was 0.2 to 0.3 mm) perpendicular to the wound surface with stainless steel blades. Washing 6-8 times with sterile water to wash out starch, dyeing for 45min with 0.1% berberine, washing 2-3 times with sterile water, washing 2-3 times with 75% ethanol, washing 1-2 times with 95% ethanol, removing dye, counterstaining for 1.5min with 0.25% toluidine blue, finally washing with sterile water and 75% ethanol to remove dye, dyeing SPP, and taking pictures under a microscope by fluorescent observation. SPP cell layer thickness measurements were performed using IS Capture image software.
After being treated by agrobacterium tumefaciens, the SPP of the damaged epidermis of the potato tuber is generated at a higher speed than that of a control of aseptic water treatment, and the thickness of the cell layer shows a statistically significant difference from 9d after the callus.
5. Staining observation of polyester cork fat SPA in potato damaged tuber surface peripheral skin and measurement of cell layer thickness:
potato tubers of 3d, 5d, 9d and 12d of the calli were taken respectively, and wound tissues were cut into slices (length and width were about 1cm, and thickness was 0.2 to 0.3 mm) perpendicular to the wound surface with stainless steel blades. Washing with sterile water for 6-8 times to wash out starch, dyeing with 0.05% toluidine blue for 45min, washing with sterile water for 2-3 times, washing with 75% ethanol for 2-3 times, washing with 95% ethanol for 1-2 times, and removing the dye. The SPA can be stained by counterstaining with 1% neutral red and finally washing the dye with sterile water and 75% ethanol, and photographed under a fluorescent microscope. The thickness of SPA cell layer was measured using IS Capture image software.
After being measured and treated by agrobacterium tumefaciens, the potato tuber injured epidermis SPA has a higher generation speed than that of a control of sterile water treatment, and the cell layer thickness shows a statistically significant difference from 3d after the callus.
The accumulation of SPP and SPA can be concluded that the generation speed of the peripheral skin on the surface of the potato tuber can be accelerated by 3-5 days after the treatment of the agrobacterium tumefaciens, and the callus of the potato tuber is promoted.
Finally, it should be noted that: the foregoing description is only a preferred embodiment of the present invention, and the present invention is not limited thereto, but it is to be understood that modifications and equivalents of some of the technical features described in the foregoing embodiments may be made by those skilled in the art, although the present invention has been described in detail with reference to the foregoing embodiments. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Sequence listing
<110> Gansu agricultural university
<120> A set of Agrobacterium tumefaciens that promotes callus on post-harvest potato tubers
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<170> SIPOSequenceListing 1.0
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<213> Agrobacterium tumefaciens (Agrobacterium tumefaciens)
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ggttagctgc ctccttgcgg ttagcgcact accttcgggt aaaaccaact cccatggtgt 60
gacgggcggt gtgtacaagg cccgggaacg tattcaccgc agcatgctga tctgcgatta 120
ctagcgattc caacttcatg cactcgagtt gcagagtgca atccgaactg agatggcttt 180
tggagattag ctcgacatcg ctgtctcgct gcccactgtc accaccattg tagcacgtgt 240
gtagcccagc ccgtaagggc catgaggact tgacgtcatc cccaccttcc tctcggctta 300
tcaccggcag tccccttaga gtgcccaact aaatgctggc aactaagggc gagggttgcg 360
ctcgttgcgg gacttaaccc aacatctcac gacacgagct gacgacagcc atgcagcacc 420
tgttctgggg ccagcctaac tgaaggacaa tgtctccact gcccaaaccc cgaatgtcaa 480
gagctggtaa ggttctgcgc gttgcttcga attaaaccac atgctccacc gcttgtgcgg 540
gcccccgtca attcctttga gttttaatct tgcgaccgta ctccccaggc ggaatgttta 600
atgcgttagc tgcgccaccg aacagtatac tgcccgacgg ctaacattca tcgtttacgg 660
cgtggactac cagggtatct aatcctgttt gctccccacg ctttcgcacc tcagcgtcag 720
taatggacca gtaagccgcc ttcgccactg gtgttcctcc gaatatctac gaatttcacc 780
tctacactcg gaattccact tacctcttcc atactcaaga tacccagtat caaaggcagt 840
tccagagttg agctctggga tttcacccct gacttaaata tccgcctacg tgcgctttac 900
gcccagtaat tccgaacaac gctagccccc ttcgtattac cgcggctgct ggcacgaagt 960
tagccggggc ttcttctccg gataccgtca ttatcttctc cggtgaaaga gctttacaac 1020
cctaaggcct tcatcactca cgcggcatgg ctggatcagg cttgcgccca ttgtccaata 1080
ttccccactg ctgcctcccg taggagtttg ggccgtgtct cagtcccaat gtggctgatc 1140
atcctctcag accagctatg gatcgtcgcc ttggtaggcc tttaccccac caactagcta 1200
atccaacgcg ggccaatcct tccccgataa atctttcccc cgtagggcgt atgcggtatt 1260
aattccagtt tcccggagct attccgcagg aaagggtatg ttcccacgcg ttactcaccc 1320
gtctgccact ccccttgcgg ggcgttcgac ttgcatgtgt taagcctgcc cgccagcgtt 1380
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Claims (8)
1. An agrobacterium tumefaciens Agrobacterium tumefaciens for promoting callus of picked potato tubers, which is characterized in that: the microbial strain is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC No:24362.
2. the agrobacterium tumefaciens Agrobacterium tumefaciens bacterial liquid for promoting the callus of the picked potato tubers is characterized in that: is obtained by culturing the Agrobacterium tumefaciens Agrobacterium tumefaciens strain according to claim 1 in an expanded manner.
3. The agrobacterium tumefaciens Agrobacterium tumefaciens bacterial liquid according to claim 2, wherein the bacterial liquid is characterized by: the preparation method of the bacterial liquid comprises the following steps: agrobacterium tumefaciens Agrobacterium tumefaciens strain is inoculated in a culture medium and shake-cultured at 37+ -1deg.C.
4. The agrobacterium tumefaciens Agrobacterium tumefaciens bacterial liquid of claim 3, wherein: the culture medium is as follows: peptone 5g/L, yeast extract 1g/L, naCl 5g/L, pH 7.0.
5. Use of the agrobacterium tumefaciens Agrobacterium tumefaciens strain of claim 1 or the agrobacterium tumefaciens Agrobacterium tumefaciens bacterial liquid of claim 2 in the preparation of a product for promoting callus of post-harvest potato tubers.
6. The use according to claim 5, characterized in that: the promotion of the callus of the picked potato tuber is specifically to accelerate the formation and thickening of the secondary peripheral skin of the picked potato tuber.
7. A method for promoting callus of post-harvest potato tubers, comprising: the agrobacterium tumefaciens Agrobacterium tumefaciens strain according to claim 1 or the agrobacterium tumefaciens Agrobacterium tumefaciens strain according to claim 2 is sprayed on the surface of damaged or freshly harvested fresh potato tubers, and the potato tubers are preserved in dark at room temperature after being dried.
8. A biogenic callus promoter for potatoes, the active ingredients of which comprise the agrobacterium tumefaciens Agrobacterium tumefaciens strain of claim 1 or the agrobacterium tumefaciens Agrobacterium tumefaciens bacterial liquid of claim 2.
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