CN114736786A - Integrated device for helicobacter pylori culture, identification and drug sensitivity experiment - Google Patents

Integrated device for helicobacter pylori culture, identification and drug sensitivity experiment Download PDF

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Publication number
CN114736786A
CN114736786A CN202210415539.8A CN202210415539A CN114736786A CN 114736786 A CN114736786 A CN 114736786A CN 202210415539 A CN202210415539 A CN 202210415539A CN 114736786 A CN114736786 A CN 114736786A
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culture
identification
helicobacter pylori
integrated device
tube
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唐晓磊
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/18—Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/06—Tubular
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability

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Abstract

The invention discloses an integrated device for helicobacter pylori culture, identification and drug sensitivity experiments, which comprises a closed container with a culture area, wherein the top of the culture area is provided with an openable and closable sample adding hole; gas producing areas are respectively arranged on two sides of the closed container, and the gas producing areas are provided with gas ports for introducing gas into the culture areas; a plurality of culture tubes which are mutually independent are vertically arranged in the culture area, and each culture tube is communicated with the sample adding hole through a liquid through tube; in this application accessible adds the sample hole and adds every culture tube with helicobacter pylori when using, cultivates helicobacter pylori through mutually independent culture tube, can directly appraise and the quick experiment of medicine in culture tube after the cultivation, only needs one set of device can accomplish cultivation, appraisal and the quick experiment of medicine to helicobacter pylori, simple structure to avoided drawing materials the interference that the pollution brought, convenient operation.

Description

Integrated device for helicobacter pylori culture, identification and drug sensitivity experiment
Technical Field
The invention relates to the technical field of kits, in particular to an integrated device for helicobacter pylori culture, identification and drug sensitivity experiments.
Background
At present, different devices are respectively adopted for culturing, identifying and drug sensitivity experiments of helicobacter pylori. Among them, the culture of helicobacter pylori is mainly a commercial kit, but the culture is limited to the isolation of a single colony, which is low in efficiency of mass amplification of a trace amount of bacteria. At present, the inoculated plate is placed in a microaerophilic tank for closed culture, and the culture mode needs a special instrument: nitrogen, oxygen, carbon dioxide and microaerophilic incubators, the structure is relatively complex. The helicobacter pylori is generally identified by combining a gram staining method with a urease test, namely gram-negative helicobacter (gull wing) and urease test positive under the observation of a microscope. The helicobacter pylori drug sensitivity test is mainly characterized in that a drug sensitivity paper sheet is pasted after the separated helicobacter pylori bacterial colony is densely scribed, the size of a bacteriostatic ring is measured, and the drug sensitivity paper sheet is interpreted according to a reference value provided by pharmacopoeia.
Therefore, the traditional culture, identification and drug sensitive experiment of helicobacter pylori all need different special equipment, the culture period is long, the pollution is easy, and the operation and result reading are tedious.
Disclosure of Invention
In view of the defects in the prior art, the invention aims to provide an integrated device for helicobacter pylori culture, identification and drug sensitivity experiment.
The technical scheme adopted by the invention is as follows:
an integrated device for helicobacter pylori culture, identification and drug sensitivity experiments comprises a closed container with a culture area, wherein the top of the culture area is provided with an openable and closable sample adding hole; gas producing areas are respectively arranged on two sides of the closed container, and the gas producing areas are provided with gas ports for introducing gas into the culture areas; the culture area is vertically provided with a plurality of mutually independent culture tubes, and each culture tube is communicated with the sample adding hole through a liquid through tube.
Further, the culture area is divided into an identification area and a drug sensitive area, and the identification area and the drug sensitive area correspond to a plurality of culture tubes.
Further, an oxygen generating device is installed in the gas production area.
Further, the bottom of each culture tube is paved with a culture medium, and the paving thickness of the culture medium is 1 mm.
Furthermore, a moisturizing area is arranged at the bottom of the culture area and outside the culture pipe, and moisturizing liquid is contained in the moisturizing area.
Furthermore, the side wall of each culture tube is provided with at least one air hole.
Further, the culture tube comprises an upper section and a lower section fixedly connected to the lower end of the upper section, and the culture medium is laid at the bottom of the lower section.
Further, the cross section of the upper section is rectangular, and the cross section of the lower section is trapezoidal.
Furthermore, a hole plug is arranged on the sampling hole.
Further, the identification area is arranged at the edge position of the closed container.
Additional aspects and advantages of the present application will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the present application.
Drawings
In order to more clearly illustrate the detailed description of the invention or the technical solutions in the prior art, the drawings that are needed in the detailed description of the invention or the prior art will be briefly described below. Throughout the drawings, like elements or portions are generally identified by like reference numerals. In the drawings, elements or portions are not necessarily drawn to scale.
FIG. 1 is a schematic structural diagram of an integrated device for helicobacter pylori culture, identification and drug sensitivity test provided in the embodiments of the present application;
FIG. 2 is another schematic structural diagram of an integrated device for helicobacter pylori culture, identification and drug sensitivity test provided in the embodiment of the present application.
Wherein, the closed container 1, the air-entrapping area 2, the air port 21, the identification area 3, the moisture supplementing area 4, the culture tube 5, the culture medium 51, the air hole 52, the sample adding hole 6, the drug sensitive area 7 and the liquid through tube 8.
Detailed Description
Embodiments of the present invention will be described in detail with reference to specific embodiments. The following examples are only for illustrating the technical solutions of the present invention more clearly, and therefore are only examples, and the protection scope of the present invention is not limited thereby.
It is to be noted that, unless otherwise specified, technical or scientific terms used herein shall have the ordinary meaning as understood by those skilled in the art to which the invention pertains.
Referring to fig. 1-2, the integrated device for culturing, identifying and drug sensitivity testing of helicobacter pylori comprises a closed container 1 with a culture area, wherein the top of the culture area is provided with an openable and closable sample adding hole 6; gas producing areas are respectively arranged on two sides of the closed container 1, and the gas producing areas are provided with gas ports 21 for introducing gas into the culture areas; a plurality of mutually independent culture tubes 5 are vertically arranged in the culture area, and each culture tube 5 is communicated with the sample adding hole 6 through a liquid through tube 8.
According to the culture device, the closed container 1 with the culture area is arranged, the plurality of mutually independent culture tubes 5 are arranged in the culture area, each culture tube 5 is communicated with the sample adding hole 6 formed in the top of the closed container 1 through the liquid through tube 8, the helicobacter pylori can be added into each culture tube 5 through the sample adding hole 6 during use, the helicobacter pylori is cultured through the mutually independent culture tubes 5, identification and drug sensitive experiments can be directly carried out in the culture tubes 5 after culture, the culture, identification and drug sensitive experiments on the helicobacter pylori can be completed only by one set of device, the structure is simple, interference caused by material taking pollution is avoided, and the operation is convenient; by respectively arranging the gas producing areas at the two sides of the closed container 1, certain oxygen can be provided for the culture area through the gas producing areas in the using process, and a microaerophilic environment is created for the culture of the helicobacter pylori.
The closed container 1 may have a cubic structure, such as a rectangular parallelepiped, a cube, or the like. Produce the gas zone and set up respectively in the both sides of closed container 1, install oxygenerator such as oxygen machine in producing the gas zone, produce oxygen through this oxygenerator, the oxygen of production lets in the cultivation district through the gas port 21 that sets up at producing the gas zone lateral wall in, builds the oxygen environment a little for the helicobacter pylori, need not to set up special oxygenerator in addition.
In order to facilitate the identification and drug sensitive experiment of helicobacter pylori, the culture area is divided into an identification area 3 and a drug sensitive area 7, and the identification area 3 and the drug sensitive area 7 correspond to a plurality of culture tubes 5.
In the embodiment, a total of 54 culture tubes 5 are arranged in the culture area, the 54 culture tubes 5 are arranged in 6 rows and 9 columns, the top of each culture tube 5 is provided with a liquid through hole, the liquid through hole is communicated with a liquid through pipe 8, the liquid through pipe 8 is communicated with a sample adding hole 6, and helicobacter pylori samples can be added to the 54 culture tubes 5 through the sample adding hole 6.
And a hole plug is arranged in the sample adding hole 6, and after sample adding is finished, the hole plug can be plugged in the sample adding hole 6 so as to plug the sample adding hole 6 and keep the sealing property of the culture area.
The identification area 3 is arranged at the edge of the closed container 1, the identification area 3 corresponds to 3 culture tubes 5, and when the device is used, culture objects in the 3 culture tubes 5 in the identification area 3 can be identified, so that the certainty of drug sensitive experimental objects is realized.
Except 3 culture tubes 5 in the identification area 3, the areas corresponding to the remaining 51 culture tubes 5 are drug sensitive areas 7, and the remaining 51 culture tubes 5 can be used for drug sensitive experiments.
The bottom of each culture tube 5 is paved with a culture medium 51, the culture medium 51 is agarose mixed GES-I cells, the culture medium 51 is paved at the bottom of the culture tube 5 by a fluid infiltration technology, the thickness is 1 mm, and the culture medium is in a film shape, so that helicobacter pylori (especially low-concentration thalli) can be attached to the tube bottom; at least one air hole 52 is opened on the side wall of each culture tube 5.
Specifically, the method of disposing the medium 51 is as follows:
adding agarose into 0.5% PBS (Chinese name of PBS is phosphate buffer solution, pH value is 7.4), heating and mixing until agarose melts, cooling to 50 deg.C, adding GES-I cell to 104/mL to obtain culture medium 51.
Each culture tube 5 is vertically arranged, each culture tube 5 comprises an upper section and a lower section fixedly connected to the lower end of the upper section, and the culture medium 51 is laid at the bottom of the lower section. The section of the upper section of the culture tube 5 is rectangular, and a plurality of air holes 52 are arranged on the upper section of the culture tube 5; the lower section of the culture tube 5 has a trapezoidal cross section, and the culture medium 51 is laid on the lower bottom of the culture tube 5. The lower section of the culture tube 5 is arranged in the trapezoid structure, so that the contact surface of the helicobacter pylori sample and air is increased.
In order to control the humidity of the culture area, a moisturizing area 4 is arranged at the bottom of the culture area and outside the culture tube 5, moisturizing liquid can be contained in the moisturizing area 4, the moisturizing liquid can be water, and when the culture tube is used, the temperature and the humidity in the culture area can be controlled through the moisturizing area 4, so that the growth of helicobacter pylori is facilitated.
The application method of the device comprises the following steps:
culturing of helicobacter pylori: the culture medium 51 is laid on the bottom of each culture tube 5, water is added to the moisturizing area 4 at the bottom of the culture area, and helicobacter pylori is added to each culture tube 5 through the sample adding hole 6 for culture.
Identification of helicobacter pylori: identifying the three culture tubes 5 in the identification area 3 by using oxidase, catalase and urease respectively, and determining the three culture tubes 5 as helicobacter pylori if the identification results of the three culture tubes 5 are positive, thereby effectively preventing interference caused by material-drawing pollution.
Drug sensitivity test of helicobacter pylori: through to clarithromycin, metronidazole, levofloxacin, amoxicillin, tinidazole, rifampicin, tetracycline, furazolidone, as 8 antibacterial first-selected antibiotics that disinfect, through the multiple dilution method, carry out the drug sensitive detection to the helicobacter pylori that is located every culture tube 5 in the drug sensitive area 7 from high concentration to low concentration gradually, can detect the antibacterial bactericidal effect of the different concentrations of multiple antibiotic, can realize the judgement to drug sensitive through the reading of instrument to turbidity.
In this application, unless expressly stated or limited otherwise, the terms "connected," "secured," and the like are to be construed broadly and can include, for example, fixed connections, removable connections, or integral combinations thereof; may be an electrical connection; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meanings of the above terms in the present invention can be understood according to specific situations by those of ordinary skill in the art.
In the description of the present invention, numerous specific details are set forth. It is understood, however, that embodiments of the invention may be practiced without these specific details. In some instances, well-known methods, systems, and techniques have not been shown in detail in order not to obscure an understanding of this description.
In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, system, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, systems, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples and features of different embodiments or examples described in this specification can be combined and combined by one skilled in the art without contradiction.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the present invention, and they should be construed as being included in the following claims and description.

Claims (10)

1. An integrated device for helicobacter pylori culture, identification and drug sensitivity experiments is characterized by comprising a closed container with a culture area, wherein the top of the culture area is provided with an openable and closable sample adding hole; gas producing areas are respectively arranged on two sides of the closed container, and the gas producing areas are provided with gas ports for introducing gas into the culture areas; the culture area is vertically provided with a plurality of mutually independent culture tubes, and each culture tube is communicated with the sample adding hole through a liquid through tube.
2. The integrated device for helicobacter pylori culture, identification and drug susceptibility testing according to claim 1, wherein the culture region is divided into an identification region and a drug susceptibility region, and the identification region and the drug susceptibility region correspond to a plurality of culture tubes.
3. The integrated device for helicobacter pylori culture, identification and drug susceptibility testing according to claim 2, wherein an oxygen generator is installed in the gas production area.
4. The integrated device for helicobacter pylori culture, identification and susceptibility testing according to claim 2, wherein the bottom of each culture tube is laid with a culture medium with a thickness of 1 mm.
5. The integrated device for helicobacter pylori culture, identification and drug sensitivity test according to claim 2, wherein a moisture-supplementing region is arranged at the bottom of the culture region and outside the culture tube, and the moisture-supplementing region contains moisture-supplementing liquid.
6. The integrated device for helicobacter pylori culture, identification and drug sensitivity test as claimed in claim 5, wherein the side wall of each culture tube is provided with at least one air hole.
7. The integrated device for helicobacter pylori culture, identification and drug sensitivity test as claimed in claim 2, wherein the culture tube comprises an upper section and a lower section attached to the lower end of the upper section, and the culture medium is laid on the bottom of the lower section.
8. The integrated device for helicobacter pylori culture, identification and susceptibility testing according to claim 7, wherein the upper section has a rectangular cross section and the lower section has a trapezoidal cross section.
9. The integrated device for helicobacter pylori culture, identification and drug sensitivity test according to claim 2, wherein the sample application hole is provided with a plug.
10. The integrated device for helicobacter pylori culture, identification and drug sensitivity test as claimed in claim 2, wherein the identification region is disposed at a position at the edge of the closed container.
CN202210415539.8A 2022-04-20 2022-04-20 Integrated device for helicobacter pylori culture, identification and drug sensitivity experiment Pending CN114736786A (en)

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CN202210415539.8A CN114736786A (en) 2022-04-20 2022-04-20 Integrated device for helicobacter pylori culture, identification and drug sensitivity experiment

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Application Number Priority Date Filing Date Title
CN202210415539.8A CN114736786A (en) 2022-04-20 2022-04-20 Integrated device for helicobacter pylori culture, identification and drug sensitivity experiment

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114480195A (en) * 2022-02-08 2022-05-13 唐晓磊 Helicobacter pylori rapid enrichment culture reagent and preparation method thereof

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114480195A (en) * 2022-02-08 2022-05-13 唐晓磊 Helicobacter pylori rapid enrichment culture reagent and preparation method thereof
CN114480195B (en) * 2022-02-08 2024-02-27 唐晓磊 Quick helicobacter pylori enrichment culture reagent and preparation method thereof

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Application publication date: 20220712