CN114736733A - High-oxidation-resistance schizochytrium limacinum oil and preparation method thereof - Google Patents
High-oxidation-resistance schizochytrium limacinum oil and preparation method thereof Download PDFInfo
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- CN114736733A CN114736733A CN202210425556.XA CN202210425556A CN114736733A CN 114736733 A CN114736733 A CN 114736733A CN 202210425556 A CN202210425556 A CN 202210425556A CN 114736733 A CN114736733 A CN 114736733A
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B5/00—Preserving by using additives, e.g. anti-oxidants
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Abstract
The invention discloses high-oxidation-resistance schizochytrium oil and a preparation method thereof. The composite material is prepared from the following raw materials in parts by mass: 99-99.9 parts of schizochytrium limacinum oil; 0.01-0.06 part of soybean lecithin; 0.009-0.025 parts of ascorbyl palmitate; 0.02-0.2 part of vitamin E. According to the invention, the soybean lecithin, the vitamin E, the ascorbyl palmitate and the DHA schizochytrium limacinum oil are compounded to prepare the high-oxidation-resistance schizochytrium limacinum oil, the compounding of various antioxidants is superior to that of a single antioxidant, the obvious synergistic promotion effect is generated among several natural antioxidants, the oxidation resistance stability is obviously improved compared with the original oxidation resistance stability, and the shelf life is prolonged by more than four times compared with the schizochytrium limacinum oil without the compounded natural antioxidants.
Description
Technical Field
The invention belongs to the field of processing of schizochytrium limacinum oil, and particularly relates to high-oxidation-resistance schizochytrium limacinum oil and a preparation method thereof.
Background
The schizochytrium oil is prepared from schizochytrium by fermentation, wall breaking, refining and other processes. Compared with fish oil, the schizochytrium limacinum oil has the advantages of simple fatty acid composition, safe components, environment-friendly extraction mode and the like. Therefore, the algae oil replaces fish oil to become a green resource which can be utilized. The schizochytrium limacinum oil mainly comprises fatty acids such as palmitic acid, stearic acid, docosapentaenoic acid, docosahexaenoic acid (DHA) and the like, wherein the content of omega-3 polyunsaturated fatty acid is rich, and the content of DHA can reach more than 40%. Related researches prove that DHA is extremely important for the health of brain, retina, skin and kidney, has the functions of improving vision and memory, preventing Alzheimer's disease and the like, and particularly can promote the growth and development of brain cells of infants. DHA content in optic nerve cells and retina tissue can reach 40% -47%, and DHA-deficient infant or fetus brain function can be obstructed, so DHA can be added into the milk powder to promote infant brain development. In addition, middle-aged and elderly people can also improve brain function by taking DHA-rich health product. In addition, DHA is an important immune regulatory factor, and DHA is used as an endogenous precursor of a programmed death receptor and a lipid regulator, and can promote the generation of the programmed death receptor and the lipid regulator, so that the generation of inflammation-causing mediums is effectively reduced, and the DHA in diet is properly improved to improve the defense capability of cells and the anti-inflammatory and inflammation repair capabilities of a human body.
DHA is a long-chain polyunsaturated fatty acid, contains six unsaturated double bond structures, and is extremely unstable in light, heat and oxygen environments, so the schizochytrium limacinum oil is easy to be subjected to oxidative decomposition in the production and storage processes, so that the DHA content is reduced, the color, the flavor and the color of the oil are changed, harmful substances such as aldehyde and ketone are generated, the nutritional value of the oil is reduced, and the health of a human body is harmed. Therefore, it is important to find an antioxidant capable of inhibiting and retarding the oxidation of grease and improving the antioxidant stability of grease.
The antioxidants are classified into natural antioxidants and synthetic antioxidants according to their properties, and synthetic antioxidants such as t-butylhydroquinone (TBHQ), Butylhydroxytoluene (BHT), and Butylhydroxyanisole (BHA) are widely used in foods, but in recent years, it has been reported that these synthetic antioxidants contain ingredients that may cause carcinogenesis, and therefore, there is a wide prospect to replace synthetic antioxidants with antioxidants naturally occurring in some plants. The natural antioxidant is mostly from plant extracts, and polyphenol substances in the natural antioxidant can be effectively combined with substance primary oxidation products free radicals to block the progress of oxidation reaction. Vitamin E, rosemary extract, licorice extract, etc. are all excellent antioxidants for lipid products. Vitamin E is a polyphenol substance, and ascorbic acid is a substance with strong reducibility, can reduce high-valence metal ions, and has a synergistic effect on a chelating agent. Natural ascorbic acid belongs to a water-soluble antioxidant, and fat-soluble ascorbyl palmitate prepared by esterifying the natural ascorbic acid and palmitic acid can be applied to lipid products. Lecithin is also a natural antioxidant and it acts as an antioxidant by providing hydrogen atoms as receptors for free radicals, breaking the chain reaction of fatty acid oxidation or chelating metal ions in oils and fats.
Disclosure of Invention
The invention aims to overcome the technical problem of poor stability of the high-purity DHA schizochytrium oil, adopts natural, stable and green composite antioxidant to improve the stability of the schizochytrium oil, and has simple process and large-scale production.
The high-oxidation-resistance schizochytrium limacinum oil provided by the invention is prepared from the following raw materials in parts by mass: 99-99.9 parts of schizochytrium limacinum oil; 0.01-0.06 part of soybean lecithin; 0.009-0.025 parts of ascorbyl palmitate; 0.02-0.2 part of vitamin E.
Preferably: 99.58-99.9 parts of schizochytrium oil; 0.0369-0.06 parts of soybean lecithin; 0.0098-0.02 part of ascorbyl palmitate; 0.029-0.15 parts of vitamin E.
More preferably: 99.83 parts of schizochytrium oil; 0.05 part of soybean lecithin; 0.02 part of ascorbyl palmitate; 0.1 part of vitamin E.
The high-oxidation-resistance schizochytrium limacinum oil is prepared by the following steps:
1) mixing vitamin E, soybean lecithin and ascorbyl palmitate uniformly to obtain a compound antioxidant;
2) and adding the compound antioxidant into the schizochytrium limacinum oil, and performing ultrasonic stirring and uniform mixing under the condition of nitrogen filling to obtain the high-oxidation-resistance schizochytrium limacinum oil containing the compound antioxidant.
In the method, the raw materials comprise: 99-99.9 parts of schizochytrium limacinum oil; 0.01-0.06 part of soybean lecithin; 0.009-0.025 parts of ascorbyl palmitate; 0.02-0.2 part of vitamin E;
preferably: 99.58-99.9 parts of schizochytrium oil; 0.0369-0.06 parts of soybean lecithin; 0.0098-0.02 part of ascorbyl palmitate; 0.029-0.15 parts of vitamin E.
More preferably: 50 parts of schizochytrium oil; 0.025 parts of soybean lecithin; 0.01 part of ascorbyl palmitate; 0.05 part of vitamin E.
In the step 2), the specific parameters of the ultrasonic stirring are ultrasonic frequency of 150-200w, and the time is 1-10 min.
Specifically, the ultrasonic frequency is 180w, and the time is 3 min.
The content of natural antioxidant contained in the obtained high-oxidation-resistance schizochytrium limacinum oil meets the addition requirement of GB 2760-2014.
The schizochytrium limacinum oil can also be other vegetable oil, algae oil and the like according to actual requirements.
The invention has the beneficial effects that:
according to the invention, the soybean lecithin, the vitamin E, the ascorbyl palmitate and the DHA schizochytrium limacinum oil are compounded to prepare the high-oxidation-resistance schizochytrium limacinum oil, the compounding of various antioxidants is superior to that of a single antioxidant, the obvious synergistic promotion effect is generated among several natural antioxidants, the oxidation resistance stability is obviously improved compared with the original oxidation resistance stability, and the shelf life is prolonged by more than four times compared with the schizochytrium limacinum oil without the compounded natural antioxidants.
The product of the invention is green and nontoxic, and can be widely applied to industries of food, health care, medicine and the like.
Detailed Description
The present invention is described in further detail below with reference to specific embodiments, and the examples are given only for illustrating the present invention and not for limiting the scope of the present invention. The examples provided below serve as a guide for further modifications by a person skilled in the art and do not constitute a limitation of the invention in any way.
The experimental procedures in the following examples, unless otherwise indicated, are conventional and are carried out according to the techniques or conditions described in the literature in the field or according to the instructions of the products. Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.
Example 1
Firstly, 0.05g of vitamin E, 0.03g of soybean lecithin and 0.0051g of ascorbyl palmitate are mixed with one another to obtain a compound antioxidant;
and secondly, adding 50g of DHA schizochytrium limacinum oil into the compound antioxidant in the first step, and performing ultrasonic stirring and mixing uniformly under the nitrogen-filled condition, wherein the specific parameter is ultrasonic frequency 180w, and the time is 3 min. Obtaining the high oxidation resistance schizochytrium limacinum oil containing the compound antioxidant.
Example 2
In the first step, 0.030g of vitamin E, 0.03g of soybean lecithin and 0.005g of ascorbyl palmitate are mixed with each other to obtain a compound antioxidant;
and secondly, adding 50g of DHA schizochytrium limacinum oil into the compound antioxidant obtained in the first step, and performing ultrasonic stirring and mixing uniformly under the nitrogen-filled condition, wherein the specific parameter is ultrasonic frequency of 180w, and the time is 3 min. Obtaining the high oxidation resistance schizochytrium limacinum oil containing the compound antioxidant.
Example 3
Firstly, mixing 0.015g of vitamin E, 0.03g of soybean lecithin and 0.005g of ascorbyl palmitate to obtain a compound antioxidant;
and secondly, adding 50g of DHA schizochytrium limacinum oil into the compound antioxidant obtained in the first step, and performing ultrasonic stirring and mixing uniformly under the nitrogen charging condition, wherein the specific parameter is ultrasonic frequency 180w, and the time is 3 min. Obtaining the high oxidation resistance schizochytrium limacinum oil containing the compound antioxidant.
Example 4
Firstly, mixing 0.05g of vitamin E, 0.025g of soybean lecithin and 0.01g of ascorbyl palmitate to obtain a compound antioxidant;
and secondly, adding 50g of DHA schizochytrium limacinum oil into the compound antioxidant in the first step, homogenizing and mixing uniformly under the nitrogen-filled condition to obtain the high-oxidation-resistance schizochytrium limacinum oil containing the compound antioxidant.
Comparative example
Firstly, mixing 0.05g of vitamin E and 0.05g of soybean lecithin to obtain a compound antioxidant;
and secondly, adding 50g of DHA schizochytrium limacinum oil into the compound antioxidant in the first step, and performing ultrasonic stirring and mixing uniformly under the nitrogen-filled condition, wherein the specific parameter is ultrasonic frequency 180w, and the time is 3 min. Obtaining the schizochytrium oil containing the compound antioxidant.
To prove the advantageous effects of the present invention, the high antioxidant schizochytrium oil prepared in example 1, the schizochytrium oil without antioxidant and the schizochytrium oil prepared in comparative example were evaluated for their antioxidant effects by rancimat oxidation stability analysis method, and compared with schizochytrium oil (50g) to which vitamin E alone (0.1g), soybean lecithin (0.1g), and ascorbyl palmitate (0.1g) were added, and the results are shown in tables 1 to 2.
TABLE 1 antioxidant Effect of different individual antioxidants
Note: the blank group is schizochytrium limacinum oil without any antioxidant
0.2% means that 0.2g of a single antioxidant is added to 100g of schizochytrium limacinum oil;
as shown in the data in Table 1, in example 1, compared with the schizochytrium oil added with a single antioxidant and the algae oil without the antioxidant, the oxidation induction time is obviously increased and the antioxidant stability is obviously improved under the condition of 120 ℃, which indicates that the synergistic effect is generated among the antioxidants. The antioxidant stability of the schizochytrium limacinum oil can be improved by compounding several antioxidants.
TABLE 2 antioxidant Effect of different Complex antioxidants
Note: the blank group is schizochytrium limacinum oil without any antioxidant
The data in table 2 show that the high oxidation resistance schizochytrium oil prepared by compounding vitamin E, soybean lecithin and ascorbyl palmitate can effectively prolong the oxidation stability of the schizochytrium oil in the examples 1-4 and the comparative example compared with the blank group. According to the analysis of the results of each example, the high oxidation resistance schizochytrium limacinum oil prepared by compounding 0.05g of vitamin E, 0.025g of soybean lecithin, 0.01g of ascorbyl palmitate and 50g of schizochytrium limacinum oil has the best oxidation stability, and the shelf life of example 4 can reach 3415h and is prolonged by 4.6 times compared with the schizochytrium limacinum oil of the blank group by deducing from a Q10 model formula. Compared with the prior art, the method has certain advantages and certain practical significance.
The present invention has been described in detail above. It will be apparent to those skilled in the art that the invention can be practiced in a wide range of equivalent parameters, concentrations, and conditions without departing from the spirit and scope of the invention and without undue experimentation. While the invention has been described with reference to specific embodiments, it will be appreciated that the invention can be further modified. In general, this application is intended to cover any variations, uses, or adaptations of the invention following, in general, the principles of the invention and including such departures from the present disclosure as come within known or customary practice within the art to which the invention pertains.
Claims (6)
1. The high-oxidation-resistance schizochytrium limacinum oil is prepared from the following raw materials in parts by mass: 99-99.9 parts of schizochytrium limacinum oil; 0.01-0.06 part of soybean lecithin; 0.009-0.025 parts of ascorbyl palmitate; 0.02-0.2 part of vitamin E.
2. The high antioxidant schizochytrium limacinum oil of claim 1, wherein: the high-oxidation-resistance schizochytrium limacinum oil is prepared from the following raw materials in parts by mass: 99.58-99.9 parts of schizochytrium oil; 0.0369-0.06 parts of soybean lecithin; 0.0098-0.02 part of ascorbyl palmitate; 0.029-0.15 part of vitamin E.
3. The high antioxidant schizochytrium oil of claim 1 or 2, wherein: the high-oxidation-resistance schizochytrium limacinum oil is prepared from the following raw materials in parts by mass: 50 parts of schizochytrium oil; 0.025 parts of soybean lecithin; 0.01 part of ascorbyl palmitate; 0.05 part of vitamin E.
4. A method for preparing the high antioxidant schizochytrium oil of any one of claims 1-3, comprising the steps of:
1) uniformly mixing vitamin E, soybean lecithin and ascorbyl palmitate to obtain a compound antioxidant;
2) and adding the compound antioxidant into the schizochytrium limacinum oil, and performing ultrasonic stirring and uniform mixing under the condition of nitrogen filling to obtain the high-antioxidant schizochytrium limacinum oil containing the compound antioxidant.
5. The method of claim 4, wherein: in the step 2), the ultrasonic stirring parameter is ultrasonic frequency of 150-.
6. The method of claim 5, wherein: in the step 2), the ultrasonic frequency is 180w, and the time is 3 min.
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| CN202210425556.XA CN114736733A (en) | 2022-04-22 | 2022-04-22 | High-oxidation-resistance schizochytrium limacinum oil and preparation method thereof |
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| CN202210425556.XA CN114736733A (en) | 2022-04-22 | 2022-04-22 | High-oxidation-resistance schizochytrium limacinum oil and preparation method thereof |
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| CN110326674A (en) * | 2019-08-09 | 2019-10-15 | 大连工业大学 | A kind of DHA algal oil and preparation method thereof containing composite antioxidant |
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2022
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| CN106675780A (en) * | 2017-01-05 | 2017-05-17 | 天津融信蓝海生物科技有限公司 | Compound antioxidant and grease containing same |
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| CN110326674A (en) * | 2019-08-09 | 2019-10-15 | 大连工业大学 | A kind of DHA algal oil and preparation method thereof containing composite antioxidant |
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