CN114734032B - Method for preparing nano silver based on kapok extract - Google Patents
Method for preparing nano silver based on kapok extract Download PDFInfo
- Publication number
- CN114734032B CN114734032B CN202210457536.0A CN202210457536A CN114734032B CN 114734032 B CN114734032 B CN 114734032B CN 202210457536 A CN202210457536 A CN 202210457536A CN 114734032 B CN114734032 B CN 114734032B
- Authority
- CN
- China
- Prior art keywords
- kapok
- nano silver
- solution
- extract
- ultrasonic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 title claims abstract description 65
- 244000146553 Ceiba pentandra Species 0.000 title claims abstract description 52
- 235000003301 Ceiba pentandra Nutrition 0.000 title claims abstract description 52
- 238000000034 method Methods 0.000 title claims abstract description 26
- 239000000284 extract Substances 0.000 title claims abstract description 18
- 239000000469 ethanolic extract Substances 0.000 claims abstract description 10
- 238000006243 chemical reaction Methods 0.000 claims abstract description 9
- 239000000843 powder Substances 0.000 claims description 13
- 238000001914 filtration Methods 0.000 claims description 7
- 238000003756 stirring Methods 0.000 claims description 7
- 229920000742 Cotton Polymers 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 101710134784 Agnoprotein Proteins 0.000 claims description 4
- 238000004140 cleaning Methods 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 4
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims description 4
- 238000010438 heat treatment Methods 0.000 claims description 4
- 238000005406 washing Methods 0.000 claims description 4
- 238000001816 cooling Methods 0.000 claims description 3
- 238000010992 reflux Methods 0.000 claims description 3
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 3
- 239000012498 ultrapure water Substances 0.000 claims description 3
- 238000000643 oven drying Methods 0.000 claims description 2
- 238000010298 pulverizing process Methods 0.000 claims description 2
- 238000009210 therapy by ultrasound Methods 0.000 claims description 2
- 239000002245 particle Substances 0.000 abstract description 16
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 12
- 239000003638 chemical reducing agent Substances 0.000 abstract description 6
- 239000000126 substance Substances 0.000 abstract description 5
- FOIXSVOLVBLSDH-UHFFFAOYSA-N Silver ion Chemical compound [Ag+] FOIXSVOLVBLSDH-UHFFFAOYSA-N 0.000 abstract description 4
- 239000003381 stabilizer Substances 0.000 abstract description 4
- 238000002156 mixing Methods 0.000 abstract description 2
- 229910052709 silver Inorganic materials 0.000 abstract description 2
- 239000004332 silver Substances 0.000 abstract description 2
- -1 silver ions Chemical class 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 48
- 241000588724 Escherichia coli Species 0.000 description 13
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 13
- 241000191967 Staphylococcus aureus Species 0.000 description 13
- SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(1+) nitrate Chemical compound [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 description 12
- 238000006722 reduction reaction Methods 0.000 description 7
- 230000002401 inhibitory effect Effects 0.000 description 6
- 229910001961 silver nitrate Inorganic materials 0.000 description 6
- 230000005764 inhibitory process Effects 0.000 description 5
- 230000009467 reduction Effects 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 241000219146 Gossypium Species 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- 239000011259 mixed solution Substances 0.000 description 4
- 229920001817 Agar Polymers 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000007865 diluting Methods 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000031700 light absorption Effects 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 241000191386 Bombax Species 0.000 description 1
- 244000061474 Bombax ceiba Species 0.000 description 1
- 235000004480 Bombax malabaricum Nutrition 0.000 description 1
- 241001164374 Calyx Species 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 235000009438 Gossypium Nutrition 0.000 description 1
- 241000590002 Helicobacter pylori Species 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 102100024295 Maltase-glucoamylase Human genes 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 238000005054 agglomeration Methods 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 108010028144 alpha-Glucosidases Proteins 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000004566 building material Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000003113 dilution method Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 229940037467 helicobacter pylori Drugs 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000012567 medical material Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 231100000956 nontoxicity Toxicity 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 229940083025 silver preparation Drugs 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B22—CASTING; POWDER METALLURGY
- B22F—WORKING METALLIC POWDER; MANUFACTURE OF ARTICLES FROM METALLIC POWDER; MAKING METALLIC POWDER; APPARATUS OR DEVICES SPECIALLY ADAPTED FOR METALLIC POWDER
- B22F9/00—Making metallic powder or suspensions thereof
- B22F9/16—Making metallic powder or suspensions thereof using chemical processes
- B22F9/18—Making metallic powder or suspensions thereof using chemical processes with reduction of metal compounds
- B22F9/24—Making metallic powder or suspensions thereof using chemical processes with reduction of metal compounds starting from liquid metal compounds, e.g. solutions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y40/00—Manufacture or treatment of nanostructures
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Nanotechnology (AREA)
- Physics & Mathematics (AREA)
- General Chemical & Material Sciences (AREA)
- Condensed Matter Physics & Semiconductors (AREA)
- General Physics & Mathematics (AREA)
- Manufacturing & Machinery (AREA)
- Crystallography & Structural Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses a method for preparing nano silver based on kapok extract. Mixing the kapok ethanol extract with silver ion solution, and reacting to generate nano silver. The invention utilizes the kapok extract to reduce silver ions to prepare simple substance nano silver. According to the method of the invention, the nano silver particles with the particle size of 2-13 nm can be prepared. The method for preparing the nano silver particles by using the kapok extract is easy to implement, strong in operability and low in cost, the kapok extract is used as a reducing agent and a stabilizing agent at the same time, other chemical components are not required to be added in the reaction process, a large number of nano silver particles are prepared in a short time, the method is environment-friendly, and the prepared nano silver solution has a college antibacterial effect.
Description
Technical field:
the invention belongs to the field of biotechnology and bacteriostasis application, and particularly relates to a method for preparing nano silver based on kapok extract.
The background technology is as follows:
the nano silver (Silver nanoparticles, ag-NPs for short) is called a new generation antibacterial agent in the twenty-first century, and has the advantages of broad-spectrum and efficient antibacterial performance, durable antibacterial property, strong permeability, safety, no toxicity, repair and regeneration, and difficulty in making microorganisms generate drug resistance. Nano silver has been widely used in various medical materials such as wound dressing, mask for surgeon, medical catheter, medical surgical instrument, implantation into human tissue, etc. The nano silver-based antibacterial agent has a wide application prospect and has wide market demands in the fields of living goods, medical and health, building materials, electronic industry and the like.
Common methods for preparing nano silver include a physical reduction method, a chemical reduction method, a biological reduction method and the like. The physical reduction method is simpler, and other chemical reagents are not needed to be added generally, but the method has high equipment requirement, higher cost and higher energy consumption. The chemical reduction method is common, but most of the used reducing agents and stabilizing agents are toxic agents, severely pollute the environment, and have high requirements on the preparation technology because the reaction conditions are strictly controlled.
The cotton (Bombax malabaricum DC) is also called Panzhihua, spotted Zhihua, hero flower, etc., which belongs to the cotton tree of the genus Gossypium of the family Gossypaceae, and is mostly distributed in the southern area of China, and is now the urban flower in Guangzhou. The flower of kapok has the functions of clearing spleen heat, protecting liver, resisting inflammation, resisting oxidation, resisting virus, resisting helicobacter pylori, resisting alpha-glucosidase, reducing blood sugar, reducing blood fat and the like.
The invention comprises the following steps:
the invention aims to provide a simple, convenient and easy method for preparing nano silver based on kapok extract, which has strong operability, low cost and environmental friendliness, and explores the antibacterial effect of the method.
The method for preparing nano silver based on kapok extract provided by the invention comprises the following steps:
mixing the kapok ethanol extract with silver ion solution, and reacting to generate nano silver.
Preferably, the kapok ethanol extract is obtained by drying and crushing kapok petals, reflux-extracting kapok powder with an ethanol water solution, ultrasonically cleaning, and filtering.
Preferably, the specific steps are as follows:
(1) Washing the cotton petals with water, oven drying, and pulverizing with pulverizer to obtain cotton powder;
(2) According to mass volume ratio=1g: adding kapok powder into 70% ethanol water solution according to the proportion of 20-30 ml, refluxing at 90 ℃ for 2 hours, continuously stirring, cooling to room temperature, performing ultrasonic treatment, and filtering to obtain kapok ethanol extract;
(3) 2-30 mmol/L AgNO 3 The volume ratio of the solution to the kapok ethanol extract is 2-10: 1, heating and stirring at 60-95 DEG CThe nano silver solution is prepared after 30 min-2 h.
Preferably, the kapok petals are washed with water, namely the kapok petals are washed with ultrapure water for 3 times.
Preferably, the drying is carried out at 65 ℃ for 1-2 d.
Preferably, the kapok powder is kapok powder which is crushed by a crusher and then sieved by a 40-mesh sieve.
Preferably, the ultrasonic is ultrasonic machine cleaning, the ultrasonic power is 350W, the ultrasonic time is 20min, and the ultrasonic temperature is 25 ℃.
Preferably, the kapok ethanol extract obtained by the filtration is filtered by using a 0.22 mu M organic filter membrane.
Preferably, the step (3) is to make AgNO with concentration of 2-30 mmol/L 3 The volume ratio of the solution to the kapok extract is 10:1, and heating and stirring at 60-95 ℃ in dark for reaction for 30 min-2 h to prepare the nano silver solution.
The invention utilizes the kapok extract to reduce silver ions to prepare simple substance nano silver. According to the method provided by the invention, the nano silver particles with the particle size of 2-13 nm can be prepared, and are spherical and plump and have good dispersibility. The method for preparing the nano silver particles by using the kapok extracting solution is easy to implement, strong in operability and low in cost, the kapok extracting solution is used as a reducing agent and a stabilizing agent at the same time, other chemical components are not required to be added in the reaction process, a large number of nano silver particles are prepared in a short time, and the method is a simple and environment-friendly nano silver preparation method, and the synthesized nano silver solution has high-efficiency antibacterial performance.
The safety and operability of synthesizing nano silver by using the kapok extract are high. The nano silver is synthesized by adopting plant extract reduction, and has the advantages of available local materials, mild reaction conditions, low cost, low toxicity, difficult agglomeration of products, less pollution and the like. At present, few researches on reduction synthesis of nano silver by using kapok extracting solution are carried out at home, the kapok extracting solution is used as a reducing agent and a stabilizing agent at the same time, other chemical components are not needed to be added, a large number of nano silver particles are prepared in a short time, and the synthesized nano silver solution has high-efficiency antibacterial performance.
Drawings
FIG. 1 is an ultraviolet-visible light absorption diagram of an ethanol extract of kapok (a), a nano silver solution (b) and a silver nitrate solution (c) of example 1;
FIG. 2 is a graph (A) of a nano-silver solution transmission electron microscope and a distribution graph (B) of nano-silver particle size in example 1;
FIG. 3 is a graph showing the antibacterial effect of the nano silver solution synthesized in example 1 on Escherichia coli;
FIG. 4 is a graph showing the bacteriostatic effect of the nano-silver solution synthesized in example 1 on Staphylococcus aureus;
FIG. 5 is a graph showing the antibacterial effect of the nano-silver solution synthesized in example 1 on Pseudomonas aeruginosa.
FIG. 6 is a graph of Minimum Inhibitory Concentration (MIC) of the nano-silver solution synthesized in example 1 against E.coli (A), staphylococcus aureus (B) and Pseudomonas aeruginosa (C).
Detailed Description
The following examples are further illustrative of the invention and are not intended to be limiting thereof.
Example 1:
separating petals and calyx of kapok, cleaning the petals with ultrapure water for 3 times, drying at 65 ℃ for 2 days, crushing by a crusher, and sieving by a 40-mesh sieve to obtain kapok powder. Weighing 6g of kapok powder, adding 180ml of 70% ethanol water solution with volume fraction, refluxing at 90 ℃ for 2 hours, stirring continuously, and cooling to room temperature; 300W, ultrasonic time 20min at 25deg.C, 200 mesh gauze coarse filtering the extractive solution, and 0.22 μm organic filter membrane filtering the coarse filtrate to obtain kapok extractive solution. AgNO with concentration of 10mmol/L 3 The volume ratio of the solution to the kapok extract is 10:1, and heating and stirring at 70 ℃ in a dark place for reaction for 30min to prepare the nano silver solution.
FIG. 1 is an ultraviolet-visible light absorption curve of a synthesized nano silver solution, a silver nitrate extracting solution and a kapok extracting solution measured at 350-650 nm. Wherein: a is kapok extract solution; b is a nano silver solution (AgNPs) synthesized by reaction; c is silver nitrate solution. The graph shows that the synthesized nano silver has a maximum absorption peak at about 430nm, and the silver nitrate solution and the kapok extract have no special absorption peak at the position.
FIG. 2 is a graph of the transmission electron microscope of the nano silver solution (A) and the particle size distribution diagram of the nano silver particles (B). As shown in FIG. 2, the silver ion particle size of the nano silver solution prepared in the embodiment is 2-13 nm, the average particle size is 8nm, and the nano silver solution is spherical and plump and has good dispersibility.
Example 2:
this example is similar to example 1 except that the mass ratio of kapok powder to 70% ethanol aqueous solution by volume fraction is different, 6g of kapok powder is weighed and 120ml of 70% ethanol aqueous solution by volume fraction is added.
Example 3:
this example is similar to example 1, except that the silver nitrate concentration is 30, 25, 15, 10, 8, 6, 4, 2mmol/L, respectively.
Example 4:
this example is similar to example 1, except that the reaction temperatures are 60, 75, 80, 85, 90, 95 ℃.
Example 5:
this example is substantially the same as example 1, except that the reaction times are 1 and 2 hours, respectively.
Example 6:
identification of antibacterial Properties of the nanosilver solution synthesized in example 1
(1) Strain culture: inoculating Escherichia coli, staphylococcus aureus and Pseudomonas aeruginosa into MH liquid culture medium, and culturing at 37deg.C and 180r/min to logarithmic phase.
(2) And (3) bacteriostasis circle test: respectively diluting Escherichia coli, staphylococcus aureus and Pseudomonas aeruginosa in logarithmic phase to 10 8 CFU/mL, when the temperature of the sterile MH agar medium is about 50 ℃, the bacterial liquid and the MH agar medium are mixed according to 100 mu L: mix and shake well at a ratio of 20 mL. Equidistant placing 3 sterile oxford cups into a disposable sterile culture dish, pouring the mixed culture medium into a flat plate, taking out the oxford cups by using sterile forceps after the culture medium is solidified, and collecting the nano silver solution, kapok extract and raw material synthesized in the embodiment 1Adding 100 mu L of saline into the holes, reversely culturing at a constant temperature of 37 ℃ for 16 hours, measuring the diameters of the inhibition zones respectively, and taking an average value for 3 times. Each treatment was repeated 3 times.
Table 1 shows the diameters of the inhibition zones of silver nitrate solutions against E.coli, staphylococcus aureus and Pseudomonas aeruginosa. Table 1 shows that the obtained nano silver solution has high antibacterial activity on Escherichia coli, staphylococcus aureus and Pseudomonas aeruginosa compared with physiological saline and kapok extract.
TABLE 1 diameter of antibacterial zone of Nano silver solution to Escherichia coli, staphylococcus aureus and Pseudomonas aeruginosa
FIG. 3 is a graph showing the test results of the inhibition zone of the nano silver solution on Escherichia coli.
Fig. 4 is a graph of the test results of the inhibition zone of the nanosilver solution against staphylococcus aureus.
FIG. 5 is a graph showing the results of a test of the inhibition zone of a nanosilver solution against Pseudomonas aeruginosa.
Example 7: experiment of the Minimum Inhibitory Concentration (MIC) of the nanosilver solution synthesized in example 1
The nano silver solution 13000r/min obtained in example 1 was centrifuged for 20min, leaving nano silver particles, washed 3 times with an equal volume of sterile water, and the precipitate was lyophilized with a lyophilizer. 1.6mg of nano silver particle sample is weighed and dissolved in 4 mM H broth to obtain 0.4mg/mL nano silver solution. A series of concentration gradients are prepared by adopting a double gradient dilution method and sequentially diluting with stock solution: 0. 0.006, 0.013, 0.025, 0.05, 0.1, 0.2, 0.4mg/mL.
100. Mu.L of MH broth, 25. Mu.L of nano-silver broth and 6.25. Mu.L of E.coli, staphylococcus aureus or Pseudomonas aeruginosa suspension were added to 96-well plates, respectively, at which time the nano-silver concentration in the system was 0, 1.2, 2.4, 4.8, 9.5, 19, 38.1, 76.2. Mu.g/mL. Culturing in a microplate reader for 24 hr, and measuring absorbance (600 nm) every 1 hr. The results were averaged by 3 experiments performed in parallel. As shown in FIG. 6, the lowest inhibitory concentration of nano silver on Escherichia coli was 38.1. Mu.g/mL (A), the lowest inhibitory concentration of Staphylococcus aureus was 19. Mu.g/mL (B), and the lowest inhibitory concentration of Pseudomonas aeruginosa was 9.5. Mu.g/mL (C).
Example 8: experiment of the minimum sterilizing concentration (MBC) of the nano silver solution synthesized in example 1
100 mu L of the mixed solution of the non-growing escherichia coli, staphylococcus aureus or pseudomonas aeruginosa in the example 7 is respectively sucked, the mixed solution is uniformly coated in an MH agar culture medium, the mixed solution is cultured overnight in a 37 ℃ incubator, the growth condition of the strain is observed, the lowest drug concentration when bacterial colony is less than or equal to 5 is an MBC value, the mixed solution is operated for 3 times in parallel, and the average value is obtained.
As shown in Table 2, the nano silver has a minimum inhibitory concentration of 38.1. Mu.g/mL for Escherichia coli, 38.1. Mu.g/mL for Staphylococcus aureus, and 19. Mu.g/mL for Pseudomonas aeruginosa.
TABLE 2 determination of Minimum Bactericidal Concentration (MBC) of nanosilver solutions on E.coli, staphylococcus aureus and Pseudomonas aeruginosa
Note that "-" indicates colony growth and "+" indicates colony growth.
The foregoing description is only a preferred embodiment of the present invention and is not intended to limit the scope of the present invention, but rather, the present invention is obviously modified, substituted and improved within the technical principles of the present invention, and the present invention is intended to be included in the scope of the present invention.
Claims (6)
1. A method for preparing nano silver based on kapok extract is characterized in that,
(1) Washing the cotton petals with water, oven drying, and pulverizing with pulverizer to obtain cotton powder;
(2) According to mass volume ratio=1g: adding kapok powder into 70% ethanol water solution according to the proportion of 20-30 ml, refluxing at 90 ℃ for 2 hours, continuously stirring, cooling to room temperature, performing ultrasonic treatment, and filtering to obtain kapok ethanol extract;
(3) 2-30 mmol/L AgNO 3 The volume ratio of the solution to the kapok ethanol extract is 10:1, heating and stirring at 60-95 ℃ for reaction for 30 min-2 h, and obtaining the nano silver solution.
2. The method of claim 1, wherein the washing of the kapok petals with water is 3 times washing the kapok petals with ultrapure water.
3. The method of claim 1, wherein the drying is at 65 ℃ for 1-2 d.
4. The method of claim 1, wherein the kapok powder is a 40 mesh sieve kapok powder after being crushed by a crusher.
5. The method of claim 1, wherein the ultrasonic is ultrasonic machine cleaning, ultrasonic power is 350W, ultrasonic time is 20min, and ultrasonic temperature is 25 ℃.
6. The method according to claim 1, wherein the step of filtering the kapok ethanol extract is performed by using a 0.22 μm organic filter.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210457536.0A CN114734032B (en) | 2022-04-27 | 2022-04-27 | Method for preparing nano silver based on kapok extract |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210457536.0A CN114734032B (en) | 2022-04-27 | 2022-04-27 | Method for preparing nano silver based on kapok extract |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114734032A CN114734032A (en) | 2022-07-12 |
| CN114734032B true CN114734032B (en) | 2024-02-20 |
Family
ID=82283276
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210457536.0A Active CN114734032B (en) | 2022-04-27 | 2022-04-27 | Method for preparing nano silver based on kapok extract |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114734032B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116237531A (en) * | 2022-12-15 | 2023-06-09 | 陕西中医药大学 | Method for preparing nano silver material by using safflower non-medicinal part and application thereof |
| CN116392514A (en) * | 2023-03-16 | 2023-07-07 | 同济大学 | Chinese angelica nano silver and preparation method and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110142416A (en) * | 2019-01-21 | 2019-08-20 | 水利部南京水利水文自动化研究所 | A kind of method that dried orange peel extracting solution prepares nano-Ag particles |
| CN113618078A (en) * | 2021-08-05 | 2021-11-09 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | Simple method for preparing nano-silver based on frangipani extract |
| CN113828791A (en) * | 2021-08-26 | 2021-12-24 | 桂林理工大学 | Method for green preparation of silver nanoparticles from passion fruit peel extract and application of silver nanoparticles |
| CN113996800A (en) * | 2021-10-28 | 2022-02-01 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | Method for preparing nano-silver based on gynura bicolor extract |
| CN113996801A (en) * | 2021-10-28 | 2022-02-01 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | Method for preparing nano-silver based on red amaranth extracting solution |
-
2022
- 2022-04-27 CN CN202210457536.0A patent/CN114734032B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110142416A (en) * | 2019-01-21 | 2019-08-20 | 水利部南京水利水文自动化研究所 | A kind of method that dried orange peel extracting solution prepares nano-Ag particles |
| CN113618078A (en) * | 2021-08-05 | 2021-11-09 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | Simple method for preparing nano-silver based on frangipani extract |
| CN113828791A (en) * | 2021-08-26 | 2021-12-24 | 桂林理工大学 | Method for green preparation of silver nanoparticles from passion fruit peel extract and application of silver nanoparticles |
| CN113996800A (en) * | 2021-10-28 | 2022-02-01 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | Method for preparing nano-silver based on gynura bicolor extract |
| CN113996801A (en) * | 2021-10-28 | 2022-02-01 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | Method for preparing nano-silver based on red amaranth extracting solution |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114734032A (en) | 2022-07-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN114734032B (en) | Method for preparing nano silver based on kapok extract | |
| Patale et al. | O, N-carboxymethyl chitosan–zinc complex: A novel chitosan complex with enhanced antimicrobial activity | |
| Mohammadi et al. | Green synthesis, characterization and antimicrobial activity of silver nanoparticles (AgNPs) using leaves and stems extract of some plants | |
| Sumitha et al. | Biocompatible and antibacterial nanofibrous poly (ϵ-caprolactone)-nanosilver composite scaffolds for tissue engineering applications | |
| Su et al. | Preparation of cotton fabric using sodium alginate-coated nanoparticles to protect against nosocomial pathogens | |
| CN113996801B (en) | Method for preparing nano-silver based on red amaranth extracting solution | |
| Anwar et al. | Adopting a green method for the synthesis of gold nanoparticles on cotton cloth for antimicrobial and environmental applications | |
| CN110074136A (en) | A kind of copper and iron oxide and the preparation method and antibacterial applications for mixing nano particle | |
| CN105596367A (en) | Nano-silver antibacterial gel with chitosan-poloxamer as gel matrix and preparation method and application of nano-silver antibacterial gel | |
| CN111407722B (en) | Silver nanoparticle composite hydrogel, preparation method and application thereof | |
| Rostami et al. | Evaluation of application of chitosan/nano selenium biodegradable film on full thickness excisional wound healing in rats | |
| CN109014236A (en) | A kind of preparation method and applications of the Nano silver grain based on citrus pectin | |
| Praseetha et al. | Aloe-vera conjugated natural Carbon Quantum dots as Bio-enhancers to accelerate the repair of chronic wounds | |
| Asraf et al. | In situ biosynthesized silver nanoparticle-incorporated synthesized zeolite A using Orthosiphon aristatus extract for in vitro antibacterial wound healing | |
| Momeni et al. | Antimicrobial effect of silver nanoparticles synthesized with Bougainvillea glabra extract on staphylococcus aureus and Escherichia Coli | |
| CN113876949A (en) | Composite antibacterial material and preparation method and application thereof | |
| CN113618078B (en) | Simple method for preparing nano-silver based on frangipani extract | |
| Ehsani et al. | Fabrication of wound dressing cotton nano-composite coated with Tragacanth/Polyvinyl alcohol: Characterization and in vitro studies | |
| Sivakamavalli et al. | Garcinia cambogia assisted synthesis of Zno nanoparticles coupled with chitosan for antibacterial, antibiofilm, cytotoxic, anticancer and ecotoxicity assessment | |
| You et al. | Preparation of UV-responsive hydrogels based on nanocellulose and their utilization in fungicide delivery | |
| CN113996800B (en) | Method for preparing nano-silver based on gynura bicolor extract | |
| CN116726194A (en) | Porphyrin-antibiotic supermolecule nanoparticle, preparation method and application thereof | |
| CN109877340A (en) | A kind of silver nano material based on egg shell template and preparation method thereof, antibacterial applications | |
| Maxwalt et al. | Synthesis of eco-friendly nanocomposite with silver nanoparticle to increase the antimicrobial activity | |
| CN114951681A (en) | Method for preparing platinum nanoparticles by adopting cordyceps flower extract |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |