CN113828791A - Method for green preparation of silver nanoparticles from passion fruit peel extract and application of silver nanoparticles - Google Patents
Method for green preparation of silver nanoparticles from passion fruit peel extract and application of silver nanoparticles Download PDFInfo
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- FOIXSVOLVBLSDH-UHFFFAOYSA-N Silver ion Chemical compound [Ag+] FOIXSVOLVBLSDH-UHFFFAOYSA-N 0.000 title claims abstract description 43
- 244000288157 Passiflora edulis Species 0.000 title claims abstract description 39
- 235000000370 Passiflora edulis Nutrition 0.000 title claims abstract description 39
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- 238000000034 method Methods 0.000 title claims abstract description 23
- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(1+) nitrate Chemical compound [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 claims abstract description 32
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 21
- 229910001961 silver nitrate Inorganic materials 0.000 claims abstract description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 16
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- 241000588724 Escherichia coli Species 0.000 description 3
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- NWZSZGALRFJKBT-KNIFDHDWSA-N (2s)-2,6-diaminohexanoic acid;(2s)-2-hydroxybutanedioic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O.NCCCC[C@H](N)C(O)=O NWZSZGALRFJKBT-KNIFDHDWSA-N 0.000 description 2
- 238000000862 absorption spectrum Methods 0.000 description 2
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- IKDUDTNKRLTJSI-UHFFFAOYSA-N hydrazine monohydrate Substances O.NN IKDUDTNKRLTJSI-UHFFFAOYSA-N 0.000 description 2
- PHTQWCKDNZKARW-UHFFFAOYSA-N isoamylol Chemical compound CC(C)CCO PHTQWCKDNZKARW-UHFFFAOYSA-N 0.000 description 2
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- 229910052709 silver Inorganic materials 0.000 description 2
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- 229920001661 Chitosan Polymers 0.000 description 1
- XDTMQSROBMDMFD-UHFFFAOYSA-N Cyclohexane Chemical compound C1CCCCC1 XDTMQSROBMDMFD-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 244000302512 Momordica charantia Species 0.000 description 1
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- 235000009812 Momordica cochinchinensis Nutrition 0.000 description 1
- 235000018365 Momordica dioica Nutrition 0.000 description 1
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 1
- 238000003917 TEM image Methods 0.000 description 1
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 1
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- 150000002212 flavone derivatives Chemical class 0.000 description 1
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- -1 silver ions Chemical class 0.000 description 1
- NGBNXJUWQPLNGM-UHFFFAOYSA-N silver;azane Chemical compound N.[Ag+] NGBNXJUWQPLNGM-UHFFFAOYSA-N 0.000 description 1
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- B22F—WORKING METALLIC POWDER; MANUFACTURE OF ARTICLES FROM METALLIC POWDER; MAKING METALLIC POWDER; APPARATUS OR DEVICES SPECIALLY ADAPTED FOR METALLIC POWDER
- B22F9/00—Making metallic powder or suspensions thereof
- B22F9/16—Making metallic powder or suspensions thereof using chemical processes
- B22F9/18—Making metallic powder or suspensions thereof using chemical processes with reduction of metal compounds
- B22F9/24—Making metallic powder or suspensions thereof using chemical processes with reduction of metal compounds starting from liquid metal compounds, e.g. solutions
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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Abstract
The invention provides a method for green preparation of silver nanoparticles from passion fruit peel extract, which comprises the following specific steps: cleaning passion fruit peel, air drying, pulverizing with a juicer, adding distilled water, heating in a water bath, cooling, filtering to obtain extractive solution, and storing in a refrigerator; preparing silver nitrate solution and sodium hydroxide solution; adding a sodium hydroxide solution into the extracting solution for constant volume, adding a silver nitrate solution after dissolution, and standing after microwave-assisted reaction; preferentially selecting reaction conditions according to the absorption peak value of ultraviolet 410nm, centrifuging the reacted liquid at a high speed, taking the precipitate, adding distilled water, repeatedly centrifuging, and drying the precipitate in a 50 ℃ oven to obtain the silver nanoparticles. The method has the advantages of simple process, low cost and short reaction time, and avoids the use of toxic reducing agents. The prepared silver nanoparticles have good stability, good antibacterial performance and wide biological activity. Therefore, the invention has wide application prospect and can provide scientific basis for the research of medical antibacterial materials.
Description
Technical Field
The invention belongs to the technical field of metal nanoparticle preparation, and particularly relates to a preparation method and application of silver nanoparticles synthesized by passion fruit peel extracting solution.
Background
Nanotechnology is one of the most compelling topics. It plays an important role in various fields such as food, medicine, agriculture and the like. Because of its unique and ever increasing availability. With the development of nanotechnology, metal nanoparticles are widely used for the construction of nanocarrier systems. Among them, silver nanoparticles have high biocompatibility, low toxicity, membrane permeability, optical surface characteristics and broad-spectrum antibacterial activity, and thus have become the subject of the greatest scientific research interest and the widest application.
In most cases, silver nanoparticles are chemically synthesized. Zhaotong et al (2006) used crown ether cross-linked chitosan as adsorbent and protective agent, and prepared nano silver by reducing silver nitrate with hydrazine hydrate in aqueous medium. Lelinbo et al (2004) mix cyclohexane, isoamyl alcohol, sodium dodecyl sulfate and water in a certain proportion to prepare a uniform and transparent inverse microemulsion system with stable thermodynamic properties, then add silver ammonium salt and hydrazine hydrate solution with a certain concentration into the inverse microemulsion respectively according to equal volume, and prepare nano silver particles with the particle size of 20-30 nm at normal temperature. Many reducing agents in the chemical method for preparing the nano silver can cause potential environmental damage and biological pollution, and can also have certain harm to human bodies, and the preparation cost is higher. And Minakshi (2018) utilizes the balsam pear extract to react with silver nitrate to prepare the nano silver particles in a green way. And the sunoline and the like (2018) adopt pectin as a reducing agent and a stabilizing agent to prepare the nano silver particles through microwave-assisted reaction. The Arunrat (2017) takes silver nitrate as a precursor, and uses Bell leaf extract as a reducing agent and a stabilizing agent to synthesize the nano-silver. A new synthetic route based on the green chemical principle becomes a research hotspot of the current method for preparing the nano silver. The synthesis of silver nanoparticles using plant extracts as reducing agents and stabilizers is the most common method for green production of nanoparticles. It has a special advantage that the plants are widely distributed, easily available and safer to handle. Therefore, the synthesis of silver nanoparticles by using plant extracts is the best environmental-friendly alternative to the traditional chemical synthesis method.
The invention adopts passion fruit peel extract to prepare and synthesize nano-silver. The pericarp of passion fruit contains various bioactive substances, such as pectin, polysaccharide, polyphenol, flavonoid, etc. These substances can react with silver nitrate to generate nano-silver. And dissolving pectin and other substances in the peel extracting solution by adding alkali, and fully reacting the pectin and silver nitrate to prepare the nano silver particles.
In the current research of synthesizing nano silver by a green method, the antibacterial performance of nano silver particles prepared by using plant extract is unsatisfactory. The passion fruit peel extracting solution has more antibacterial active substances, so that the prepared nano silver particles have higher antibacterial performance. And the method for preparing the nano-silver by using the passion fruit peel extracting solution is not reported, so that another new idea can be provided for the preparation method of the nano-silver by the research. In addition, the antibacterial activity of the nano-silver synthesized by the method is researched, and a basis is provided for the application of the nano-silver in the field of medicine.
Disclosure of Invention
The invention aims to solve the technical problem of providing a new idea of synthesizing silver nanoparticles by using passion fruit peel extracting solution, which is simple, convenient and easy to implement, strong in operability, low in cost and environment-friendly.
In order to solve the technical problems, the invention adopts the following technical scheme:
a method for preparing silver nanoparticles from passion fruit peel extract in a green manner comprises the following steps:
(1) cleaning passion fruit peel, airing, accurately weighing 10g, crushing by using a juicer, putting into a 100mL beaker, adding 50mL of distilled water, putting into a 90 ℃ water bath kettle, heating for 20min, cooling, filtering by using gauze to obtain passion fruit peel extract, and adding distilled water to fix the volume of the extract to 50 mL. Placing the extractive solution in refrigerator, and storing at 4 deg.C.
(2) Preparing 0.1-1.0 mol/L silver nitrate solution and 1g/L sodium hydroxide solution.
(3) And taking 5-20 mL of extracting solution, adding 1g/L of sodium hydroxide solution to a constant volume of 100mL, adjusting the pH value to 11, fully dissolving, adding 2mL of silver nitrate solution, carrying out microwave-assisted reaction at a power of 100-800W for 1-3 min, and standing for 10-30 min.
(4) And (3) preferentially selecting reaction conditions according to the absorption peak value of ultraviolet 410nm, and centrifuging the reacted liquid at a high speed at a rotating speed of 10000r/min for 20 min. Taking the precipitate, adding a proper amount of distilled water, repeatedly centrifuging twice, and drying the precipitate in an oven at 50 ℃ overnight to obtain the silver nanoparticles.
The innovation points and advantages of the invention are as follows:
1. the invention uses the passion fruit peel extract dissolved by alkali as a reducing agent and a stabilizing agent to react with silver nitrate, so that silver ions are completely reduced into silver nano particles. The formed silver nano particles have good stability and good antibacterial performance.
2. The preparation method of the silver nanoparticles adopts microwave-assisted synthesis of the silver nanoparticles, has simple preparation process, low cost and short reaction time, and avoids the use of toxic reducing agents.
3. The nano silver particles of the invention have wide biological activity, not only have the biological characteristics of nano silver such as broad-spectrum antibacterial activity, but also have the biological activities of polysaccharide, polyphenol, flavone and the like in passion fruit peel. Therefore, the antibacterial material has wide application prospect as an efficient, low-cost and broad-spectrum antibacterial material, and can provide scientific basis for the research of medical antibacterial materials.
Drawings
FIG. 1 is a transmission electron micrograph (a) and a particle size distribution (b) of silver nanoparticles in example 1.
Fig. 2 is an ultraviolet-visible light absorption spectrum of the silver nanoparticles in example 1.
FIG. 3 is the minimum inhibitory concentration of silver nanoparticles on Escherichia coli (a) and Staphylococcus aureus (b) in example 1.
Detailed Description
The invention is further described below with reference to the drawings and specific preferred embodiments of the description, without thereby limiting the scope of protection of the invention. The materials and equipment used in the following examples are commercially available.
The invention discloses a method for preparing silver nanoparticles from passion fruit peel extract in an environment-friendly manner, which comprises the following steps:
the passion fruit peel extract is used as a stabilizer and a reducing agent, silver nanoparticles are synthesized by microwave assistance, and the silver nanoparticles are prepared after high-speed centrifugation. Overcomes the defects of the prior art, and provides a preparation method and application of silver nanoparticles based on passion fruit peel extract, which are environment-friendly, low in toxicity, low in cost, high in yield and strong in antibacterial activity.
Example 1
The method for preparing the nano silver particles from the passion fruit peel extracting solution comprises the following specific steps:
(1) cleaning passion fruit peel, airing, accurately weighing 10g, crushing, putting into a 100mL beaker, adding 50mL of distilled water, putting into a 90 ℃ water bath, heating for 20min, cooling, filtering to obtain a passion fruit peel extracting solution, and adding distilled water to fix the volume of the extracting solution to 50 mL;
(2) taking 10mL of passion fruit peel extracting solution into a 250mL conical flask, adding 1g/L of sodium hydroxide solution and fixing the volume to 100 mL;
(3) adding 2mL of 0.5mol/L silver nitrate solution, and uniformly stirring;
(4) putting the reaction reagent into a microwave oven with the power of 400W, heating for 2min, and standing for reaction for 20 min;
(5) centrifuging the reacted liquid at high speed under 10000r/min for 20min, collecting the precipitate, adding distilled water in equal amount, washing, and centrifuging twice;
(6) drying the precipitate product obtained in the step (5) at 50 ℃ overnight to obtain powder, namely nano silver particles;
(7) in an experiment, the reaction solution is changed from dark green transparent to brown black, which indicates that silver nanoparticles are generated, ultraviolet spectrum scanning is carried out under the condition of the wavelength of 300-600 nm, and the maximum absorption peak near 420nm is used as an optimization condition;
(8) the silver nanoparticles obtained in this example were observed by a transmission electron microscope, the transmission electron microscope image of the silver nanoparticles is shown in fig. 1(a), the particle size distribution diagram is shown in fig. 1(b), and the average particle size of the silver nanoparticles prepared in this example is 12.1nm, and the silver nanoparticles are in a full sphere shape and have good dispersibility. Fig. 2 is an ultraviolet-visible light absorption spectrum of the prepared silver nanoparticles.
Example 2
This example was similar to example 1, except that the amount of the passion fruit peel extract added (step (2)) was 5, 10, 15, and 20 mL.
Example 3
This example was similar to example 1 except that the silver nitrate concentration (step (3)) was 0.1, 0.3, 0.5 and 0.7 mol/L.
Example 4
This example was similar to example 1, except that the standing time (step (4)) after the microwave reaction was 0, 10, 20 and 30min, respectively.
Example 5
The embodiment is a method for preparing silver nanoparticles from passion fruit peel extract in an environment-friendly manner and application of the silver nanoparticles in the field of bacteriostasis, and the silver nanoparticles obtained in the embodiment 1 are used for carrying out bacteriostasis experiments.
Experiment of minimum inhibitory concentration
3.2mg of the silver nanoparticle sample obtained in example 1 was weighed and dissolved in 1mL of broth to obtain 3.2 mg/mL. A series of concentration gradients were prepared for dilution of the stock solution in this way: 0mg/mL, 0.1mg/mL, 0.2mg/mL, 0.4mg/mL, 0.8mg/mL, 1.6mg/mL, 3.2 mg/mL.
0.2mL broth, 0.05mL nano-silver broth and 0.05mL Escherichia coli or Staphylococcus aureus suspension with the concentration controlled at 105About one/mL, the mixture was mixed well and applied to a cell plate, and the nanosilver concentration in the system was 0. mu.g/mL, 16.7. mu.g/mL, 33.3. mu.g/mL, 66.7. mu.g/mL, 133.3. mu.g/mL, 266.7. mu.g/mL, 533.3. mu.g/mL. Culturing for 0h, 3h, 6h, 9h, 12h, 15h and 24h, and detecting light absorption value (600nm) with microplate reader. As shown in FIG. 3, the minimum inhibitory concentration of nano-silver to Escherichia coli was 33.3. mu.g/mL (FIG. 3a), and the minimum inhibitory concentration of Staphylococcus aureus was 66.7. mu.g/mL (FIG. 3 b).
The above description is only a preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above-described embodiments. All technical schemes belonging to the idea of the invention belong to the protection scope of the invention. It should be noted that modifications and embellishments within the scope of the invention may be made by those skilled in the art without departing from the principle of the invention, and such modifications and embellishments should also be considered as within the scope of the invention.
Claims (7)
1. A method for preparing silver nanoparticles from passion fruit peel extract in a green manner is characterized by comprising the following steps:
(1) preparing a passion fruit peel extracting solution; cleaning passion fruit peel, drying in the air, crushing by using a juicer, putting into a beaker, adding a proper amount of water, putting into a water bath kettle for heating, cooling, and filtering by using 4 layers of gauze to obtain a passion fruit peel extracting solution;
(2) adding a sodium hydroxide solution and a silver nitrate solution into the step 1;
(3) reacting the step 2 for a period of time under the assistance of microwaves;
(4) and (4) centrifuging the solution obtained in the step (3) at a high speed, and drying to obtain silver nanoparticles.
2. The method for green preparation of silver nanoparticles from passion fruit peel extract as claimed in claim 1, wherein the passion fruit peel extract in the step is prepared by taking a certain amount of passion fruit peel, crushing by a juicer, dispersing in distilled water, heating and filtering to obtain the extract.
3. The method for preparing silver nanoparticles in a green manner by using passion fruit peel extract according to claim 1, wherein the method for preparing the passion fruit peel extract alkaline solution in the step is to dissolve a certain amount of passion fruit peel extract in a sodium hydroxide solution to obtain the passion fruit peel extract alkaline solution.
4. The method for preparing silver nanoparticles from passion fruit peel extract in a green manner as claimed in claim 1, wherein the silver nitrate solution in the step is prepared by dissolving a certain amount of silver nitrate in distilled water to prepare a silver nitrate solution with a concentration of 0.1-0.5 mol/L.
5. The method for green preparation of silver nanoparticles from passion fruit peel extract according to claim 1, wherein in the step, the microwave power is 0-800W, and the microwave time is 1-10 min.
6. The method for green preparation of silver nanoparticles from passion fruit peel extract according to claim 1, wherein in the step, the centrifugation time is 10-20 min.
7. An application of the passion fruit peel extract prepared by the preparation method of any one of claims 1-6 in the field of antibacterial preparation of silver nanoparticles in a green environment.
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| Title |
|---|
| 凌绍明;: "银纳米粒子的绿色合成及其光谱特性" * |
| 杨宁;郝林;杨鹏;: "芒果皮提取物合成纳米银及抑菌性研究" * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114734032A (en) * | 2022-04-27 | 2022-07-12 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | Method for preparing nano-silver based on kapok extract |
| CN114734032B (en) * | 2022-04-27 | 2024-02-20 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | Method for preparing nano silver based on kapok extract |
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