CN114732864A - Preparation process of Tongkang tablets - Google Patents

Preparation process of Tongkang tablets Download PDF

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CN114732864A
CN114732864A CN202210227139.4A CN202210227139A CN114732864A CN 114732864 A CN114732864 A CN 114732864A CN 202210227139 A CN202210227139 A CN 202210227139A CN 114732864 A CN114732864 A CN 114732864A
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extraction
temperature
tongkang
tablets
pressure
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李海池
周雪
周丹
葛发欢
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GKH PHARMACEUTICAL Ltd
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Sun Yat Sen University
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/481—Astragalus (milkvetch)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/56—Materials from animals other than mammals
    • A61K35/618—Molluscs, e.g. fresh-water molluscs, oysters, clams, squids, octopus, cuttlefish, snails or slugs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
    • A61K36/238—Saposhnikovia
    • A—HUMAN NECESSITIES
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
    • A61K36/284—Atractylodes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/75—Rutaceae (Rue family)
    • A61K36/752—Citrus, e.g. lime, orange or lemon
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/88—Liliopsida (monocotyledons)
    • A61K36/894—Dioscoreaceae (Yam family)
    • A61K36/8945—Dioscorea, e.g. yam, Chinese yam or water yam
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/37—Extraction at elevated pressure or temperature, e.g. pressurized solvent extraction [PSE], supercritical carbon dioxide extraction or subcritical water extraction
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids

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Abstract

The invention discloses a process for preparing tongkang tablets. The invention adopts supercritical CO2The extraction technology extracts volatile components of the medicinal materials, and the extracted residue and other medicinal materials are crushed at low temperature and high pressure for full extraction, so as to improve the original extraction method, shorten the extraction process, improve production efficiency, and realize supercritical CO extraction2The extraction technology and the low-temperature high-pressure crushing technology can extract the index components at a lower temperature, avoid the influence of high temperature on the index components in the preparation process of the product in the prior art, and reduce degradation, thereby improving the content of the index components in the final product and improving the stability and the curative effect of the product. The invention has simple and convenient operation, no pollution, low temperature and short period, can improve the content of index components, and has the same advantages as the methodThe problem of low content of atractylone in the product of the prior art is solved.

Description

Preparation process of tongkang tablets
Technical Field
The invention relates to the technical field of traditional Chinese medicine extraction and preparation, in particular to a preparation process of tongkang tablets.
Background
The tablet for children is a pure Chinese medicinal preparation, the prescription is derived from a classic and famous prescription of 'Yupingfeng powder' for treating exterior deficiency and spontaneous perspiration, the Yupingfeng powder consists of three Chinese medicaments of divaricate saposhnikovia root, astragalus and largehead atractylodes rhizome, and the tablet for children is added with Chinese yam, oyster and tangerine peel on the basis, consists of six medicinal materials of the astragalus, the largehead atractylodes rhizome, the divaricate saposhnikovia root, the Chinese yam, the oyster and the tangerine peel, has the effects of tonifying lung and consolidating exterior, strengthening spleen and tonifying stomach and improving organism immunity, and is suitable for children patients with the lung and spleen qi deficiency syndrome caused by repeated respiratory tract infection of children.
The primordin, calycosin glucoside, 5-O-methylvisamicoside and hesperidin are main index components of Tongkang preparation, and have reduced stability at high temperature. Atractylone has effects of protecting liver and inhibiting CCl4The pharmacological actions of lipid peroxidation, cytotoxic activity, anti-tumor, anti-inflammatory and the like caused by the traditional Chinese medicine are one of the effective components of the bighead atractylodes rhizome. Atractylone is unstable at room temperature, is easily oxidized into atractylenolide I and atractylenolide III, and has accelerated degradation speed under the heating condition.
In the existing preparation process of Tongkang tablets, water vapor distillation is adopted to extract volatile oil in bighead atractylodes rhizome and divaricate saposhnikovia root, the distillation time reaches more than 6 hours, the liquid medicine is filtered and stored separately after the volatile oil is collected, and the decoction dregs are decocted again by adding water; decocting radix astragali and Concha Ostreae in water for 2 times, filtering, mixing all decoctions, and concentrating under reduced pressure to obtain soft extract. In the process of extraction and preparation at high temperature for a long time, index components and atractylone are easy to oxidize and degrade, so that the index components in the Tongkang preparation product are reduced, the content of atractylone is extremely low, and the quality control of the product is not facilitated. Through literature retrieval, at present, published research is based on extraction and analysis of index components in single medicinal materials, and supercritical CO adopted is not found2The fluid technology is used for extracting and preparing volatile oil of the tongkang tablet medicinal material and the low-temperature high-pressure crushing extraction technology is used for extracting and preparing the tongkang tablet medicinal material, and the influence of the extraction process on subsequent products is not considered.
Disclosure of Invention
The invention provides a preparation process of Tongkang tablets, aiming at reducing the influence of temperature on the extraction and preparation process of Tongkang tablets and improving the content of index components and atractylone in the product. The process adopts supercritical CO2The extraction technology combines the high-pressure crushing extraction technology to improve the extraction mode of medicinal materials, reduce the extraction temperature and shorten the extraction time to improve the content of the index components and the atractylone in the tongkang tablets, and the specific technical scheme is as follows:
a preparation process of Tongkang tablets comprises the following steps:
(1) crushing medicinal materials: pulverizing radix astragali, rhizoma Dioscoreae, pericarpium Citri Tangerinae, Atractylodis rhizoma, radix Saposhnikoviae, and Concha Ostreae respectively;
(2) supercritical CO of volatile oil2And (3) extraction: placing the crushed rhizoma atractylodis macrocephalae, radix sileris and dried orange peel into a supercritical extraction kettle, respectively heating and boosting an extraction device to extraction conditions, starting circular extraction, keeping constant temperature and constant pressure for required extraction time, and obtaining volatile oil and dregs of the rhizoma atractylodis macrocephalae, radix sileris and dried orange peel after extraction is finished;
(3) crushing and extracting water-soluble components at low temperature and high pressure: adding the crushed astragalus, Chinese yam and oyster into the dregs obtained in the step (2), adding water, crushing and extracting at low temperature and high pressure, filtering the extracting solution to obtain filtrate, and concentrating under reduced pressure to obtain thick paste;
(4) granulating and drying: mixing the volatile oil extracted in step (2) and the thick paste obtained in step (3), adding adjuvants, granulating, drying, and tabletting.
Further, the formula of the medicinal materials in the step (1) comprises the following components in parts by mass: 300-350 parts of astragalus membranaceus, 300-350 parts of Chinese yam, 20-30 parts of dried orange peel, 200-220 parts of bighead atractylodes rhizome, 100-115 parts of divaricate saposhnikovia root and 300-350 parts of oyster.
Further, in the step (1), the crushing granularity of the medicinal materials is 60-100 meshes.
Further, in the step (2), supercritical CO2The extraction conditions were: the extraction pressure is 15-30 MPa, and the extraction temperature is 40-60 ℃; the pressure of an analysis kettle I is 6-12 MPa, and the temperature of the analysis kettle I is 45-55 ℃; the pressure of the resolution kettle II is 6-8 MPa, and the temperature of the resolution kettle II is 45-50 ℃; and circularly extracting for 30-75 min.
Further, in the step (2), the yield of the volatile oil is more than 1.5%, and the content of atractylone is more than 1.4%.
Further, in the step (3), the conditions of low-temperature high-pressure crushing extraction are as follows: the extraction pressure is 50-120 MPa; the extraction temperature is 4-24 ℃.
Further, in the step (3), the addition amount of water is 10-30 mL per gram of the mixed medicinal materials.
Further, in the step (3), the yield of the thick paste is more than 40%.
Further, in the step (4), calculated by dry products, the content of the cimicidin in the granules is more than or equal to 0.4769mg/2g, the content of calycosin glucoside is more than or equal to 0.6384mg/2g, the content of 5-O-methylvisammioside is more than or equal to 0.7591mg/2g, the content of hesperidin is more than or equal to 4.1723mg/2g, and the content of atractylone is more than or equal to 0.3205mg/2 g.
Compared with the prior art, the invention has the following advantages and effects:
the invention adopts supercritical CO2The extraction technology extracts volatile components of the medicinal materials, and the extracted residue and other medicinal materials are crushed at low temperature and high pressure for full extraction, so as to improve the original extraction method, shorten the extraction process, improve production efficiency, and realize supercritical CO extraction2The extraction technology and the low-temperature high-pressure crushing technology can extract the index components at a lower temperature, avoid the influence of high temperature on the index components in the preparation process of the product in the prior art, and reduce degradation, thereby improving the content of the index components in the final product and improving the stability and the curative effect of the product.
The method has the advantages of simple and convenient operation, no pollution, low temperature and short period, can improve the content of index components, and simultaneously solves the problem of low content of atractylone in the product of the prior art.
Detailed Description
The present invention will be further specifically illustrated by the following examples for better understanding, but the present invention is not to be construed as being limited thereto, and certain insubstantial modifications and adaptations of the invention by those skilled in the art based on the foregoing disclosure are intended to be included within the scope of the invention.
The component detection and analysis method related in the embodiment of the invention comprises the following steps:
(1) index component detection and analysis method
A chromatographic column: chromatographic column Kromasil 100-5-C18(250mm × 4.6mm, 5 μm), flow rate of 1ml/min, column temperature of 25 deg.C, detection wavelength of cimicidin, calycosin glucoside, 5-O-methylvisamicosin (260nm), hesperidin 283nm, and mobile phase of acetonitrile-01% formic acid solution, gradient elution according to the procedure shown in Table 1.
Table 1 index component gradient elution procedure:
Figure BDA0003536043060000031
(2) atractylone detection and analysis method
Chromatographic conditions are as follows: chromatographic column Kromasil 100-5-C18(250 mm. times.4.6 mm, 5 μm), a flow rate of 1ml/min, a column temperature of 35 ℃, a detection wavelength of 220nm, and a mobile phase of acetonitrile-water, and gradient elution was performed according to the procedure shown in Table 2.
TABLE 2 atractylone elution gradient
Figure BDA0003536043060000032
Figure BDA0003536043060000041
(3) Preparation of test solution
Preparing a volatile oil test solution: precisely weighing 25mg of volatile oil, dissolving in methanol, diluting to 10ml, shaking, precisely transferring 1ml, diluting with methanol 1 times, and shaking to obtain volatile oil sample solution.
Preparing a finished product test solution: weighing 2g of the finished product, precisely weighing, placing in a 50ml conical flask with a plug, precisely adding 20ml of methanol, extracting under heating and refluxing for 60min, cooling, weighing again, supplementing the weight loss with methanol, shaking up, filtering with a 0.45 μm microporous membrane, and taking the subsequent filtrate as the finished product sample solution.
(4) Preparation of control solutions
Respectively and precisely weighing appropriate amount of reference substances of the cimicidin, the calycosin glucoside, the 5-O-methylvisammol glucoside, the hesperidin and the atractylone, putting the reference substances into a 2ml volumetric flask, adding methanol to dissolve the reference substances, and fixing the volume to a scale to prepare reference substance mother liquor containing the cimicidin 0.5309mg/ml, the calycosin glucoside 0.4852mg/ml, the 5-O-methylvisammol glucoside 0.49816mg/ml, the hesperidin 0.4949mg/ml and the atractylone 0.5194 mg/ml. Respectively transferring appropriate amount of mother liquor of each reference substance, and diluting to reference substance solutions with different concentrations.
(5) Linear relationship and range
According to the chromatographic conditions, respectively and precisely sucking 10 μ l of each of the reference solution and the test solution, injecting into a liquid chromatograph, and recording the chromatographic peak diagram. Calculating according to the peak area by an external standard method to obtain the product. The results are shown in Table 3.
TABLE 3 Linear relationship
Figure BDA0003536043060000042
Example 1
The formula is as follows: 210 parts of bighead atractylodes rhizome, 110 parts of divaricate saposhnikovia root, 25 parts of dried orange peel, 330 parts of astragalus, 325 parts of Chinese yam and 320 parts of oyster, wherein the parts refer to the parts by mass;
the bighead atractylodes rhizome, the divaricate saposhnikovia root, the dried orange peel, the astragalus root, the Chinese yam and the oyster are respectively crushed into 60-100 meshes.
(1) Supercritical extraction
Taking the crushed medicinal materials of the bighead atractylodes rhizome, the divaricate saposhnikovia root and the dried orange peel, sampling according to the prescription amount, placing the medicinal materials in a supercritical extraction kettle, and adjusting the extraction pressure to 15-30 MPa and the extraction temperature to 40-60 ℃; the pressure of an analysis kettle I is 6-12 MPa, and the temperature is 45-55 ℃; and (3) circularly extracting for 30-75 min at the pressure of 6-8 MPa and the temperature of 45-50 ℃ in an analysis kettle II, and obtaining the volatile oil and the dregs of the medicinal materials of the bighead atractylodes rhizome, the divaricate saposhnikovia root and the dried orange peel after extraction. The results are shown in Table 4.
TABLE 4 results of volatile oil extraction
Figure BDA0003536043060000051
(2) High pressure difference crushing extraction
Taking the medicine residues obtained by supercritical extraction, adding the crushed astragalus, Chinese yam and oyster according to the formula ratio, uniformly mixing, weighing a proper amount, adding 10-30 ml of purified water into each gram of the medicine, performing low-temperature high-pressure crushing extraction for 1 time under the conditions that the temperature is 4-24 ℃ and the pressure is 50-120 MPa, filtering after extraction is completed, and concentrating the filtrate under reduced pressure to form thick paste. The results are shown in Table 5.
TABLE 5 Low temperature high pressure crushing extraction results
Figure BDA0003536043060000052
Supercritical CO2Mixing the extracted volatile oil (sample B) and the soft extract (sample B) obtained by crushing at low temperature and high pressure difference, adding appropriate amount of adjuvants, granulating, and drying.
Comparative example 1
The prior art comprises the following process steps:
(1) crushing medicinal materials: respectively pulverizing rhizoma Dioscoreae and pericarpium Citri Tangerinae into fine powder; pulverizing Atractylodis rhizoma and radix Saposhnikoviae to 5 mesh;
(2) fat-soluble component extraction: taking pulverized Atractylodis rhizoma and radix Saposhnikoviae, sampling according to formula, placing in round-bottom flask, boiling, extracting volatile oil by steam distillation, collecting volatile oil, storing the distilled liquid medicine, decocting the residue with water for 1 time, filtering, and mixing the liquid medicines for 2 times;
(3) water-soluble component extraction: weighing radix astragali and Concha Ostreae according to formula ratio, decocting in water for 2 times, filtering the extractive solution, mixing the extractive solutions for 2 times, mixing with the medicinal liquid in step (2), and concentrating under reduced pressure to obtain soft extract;
(4) granulating and drying: taking the thick paste in the step (3), adding the fine powder of the Chinese yam and the dried orange peel, mixing and uniformly mixing, dissolving the fat-soluble components in the step (2) in an ethanol solution with the volume fraction of 95%, wherein the weight of the fat-soluble components is as follows: mixing ethanol solution at volume ratio of 1:5(g/ml) with appropriate amount of adjuvants, granulating, drying, and tabletting.
Respectively carrying out liquid chromatography detection on the particles in the example 1 and the particles in the comparative example 1, recording peak areas according to detection results of high performance liquid chromatography, and calculating the contents of the index components and atractylone by contrasting the linear relation between the peak areas and the concentrations of the effective components in the standard solution. The details are as in table 6 below:
TABLE 6 index ingredient and atractylone content test result table for finished granule
Figure BDA0003536043060000061
In contrast, the content of 5 components in the product obtained by the improved process is higher than that of the sample obtained by the original process, which shows that the supercritical CO is obtained2The extraction can obviously improve the content of atractylone in the finished product, the low-temperature high-pressure crushing extraction can improve the extraction rate of the index components, simultaneously shorten the heating time in the processes of extracting and concentrating the medicinal materials, reduce the degradation of the index components and improve the content of the index components in the finished product, and the method provided by the invention has obvious progress.
The above-mentioned embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above-mentioned embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and they are intended to be included in the scope of the present invention.

Claims (8)

1. A preparation process of Tongkang tablets is characterized by comprising the following steps: the method comprises the following steps:
(1) crushing medicinal materials: pulverizing radix astragali, rhizoma Dioscoreae, pericarpium Citri Tangerinae, Atractylodis rhizoma, radix Saposhnikoviae, and Concha Ostreae respectively;
(2) supercritical CO of volatile oil2Extraction: placing the crushed rhizoma atractylodis macrocephalae, radix sileris and dried orange peel into a supercritical extraction kettle, respectively heating and boosting an extraction device to extraction conditions, starting circular extraction, keeping constant temperature and constant pressure for required extraction time, and obtaining volatile oil and dregs of the rhizoma atractylodis macrocephalae, radix sileris and dried orange peel after extraction is finished;
(3) crushing and extracting water-soluble components at low temperature and high pressure: adding the crushed astragalus, Chinese yam and oyster into the dregs obtained in the step (2), adding water, performing low-temperature high-pressure crushing extraction, filtering the extracting solution to obtain filtrate, and concentrating the filtrate under reduced pressure to obtain thick paste;
(4) granulating and drying: mixing the volatile oil extracted in step (2) and the thick paste obtained in step (3), adding adjuvants, granulating, drying, and tabletting.
2. The process for preparing tongkang tablets as claimed in claim 1, wherein:
the formula of the medicinal materials in the step (1) comprises the following components in parts by mass: 300-350 parts of astragalus membranaceus, 300-350 parts of Chinese yam, 20-30 parts of dried orange peel, 200-220 parts of bighead atractylodes rhizome, 100-115 parts of divaricate saposhnikovia root and 300-350 parts of oyster.
3. The process for preparing tongkang tablets as claimed in claim 1 or 2, wherein:
in the step (1), the crushing granularity of the medicinal materials is 60-100 meshes.
4. The process for preparing tongkang tablets as claimed in claim 1 or 2, wherein:
in the step (2), supercritical CO2The extraction conditions were: the extraction pressure is 15-30 MPa, and the extraction temperature is 40-60 ℃; the pressure of an analysis kettle I is 6-12 MPa, and the temperature of the analysis kettle I is 45-55 ℃; the pressure of the resolution kettle II is 6-8 MPa, and the temperature of the resolution kettle II is 45-50 ℃; and circularly extracting for 30-75 min.
5. The process for preparing tongkang tablets as claimed in claim 1 or 2, wherein:
in the step (3), the conditions of low-temperature high-pressure crushing and extraction are as follows: the extraction pressure is 50-120 MPa; the extraction temperature is 4-24 ℃; the addition amount of water is 10-30 mL per gram of the mixed medicinal materials.
6. The process for preparing tongkang tablets as claimed in claim 4, wherein:
in the step (2), the yield of the volatile oil is more than 1.5%, and the content of atractylone is more than 1.4%.
7. The process for preparing tongkang tablets as claimed in claim 5, wherein:
in the step (3), the yield of the thick paste is more than 40%.
8. The process for preparing tongkang tablets according to claim 1 or 2, wherein:
in the step (4), calculated by dry products, the content of the cimicidin in the granules is more than or equal to 0.4769mg/2g, the content of calycosin glucoside is more than or equal to 0.6384mg/2g, the content of 5-O-methylvisammioside is more than or equal to 0.7591mg/2g, the content of hesperidin is more than or equal to 4.1723mg/2g, and the content of atractylone is more than or equal to 0.3205mg/2 g.
CN202210227139.4A 2022-03-08 2022-03-08 Preparation process of Tongkang tablets Pending CN114732864A (en)

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Publication number Priority date Publication date Assignee Title
CN105758985A (en) * 2016-02-23 2016-07-13 广州康和药业有限公司 Quality inspection method of Tongkang tablets
CN110638923A (en) * 2019-11-08 2020-01-03 广州中大南沙科技创新产业园有限公司 Production process for improving atractylone content in tongkang tablets
CN111358719A (en) * 2020-03-18 2020-07-03 西北师范大学白银师科创新研究院 Chinese herbal medicine lipstick with cheilitis preventing function and preparation method thereof

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
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