CN114732560A - Method and device for constructing optic nerve injury model - Google Patents
Method and device for constructing optic nerve injury model Download PDFInfo
- Publication number
- CN114732560A CN114732560A CN202110024516.XA CN202110024516A CN114732560A CN 114732560 A CN114732560 A CN 114732560A CN 202110024516 A CN202110024516 A CN 202110024516A CN 114732560 A CN114732560 A CN 114732560A
- Authority
- CN
- China
- Prior art keywords
- ring
- optic nerve
- constructing
- pressure ring
- pressurizing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 208000030768 Optic nerve injury Diseases 0.000 title claims abstract description 29
- 238000000034 method Methods 0.000 title claims abstract description 19
- 210000001328 optic nerve Anatomy 0.000 claims abstract description 26
- 238000002347 injection Methods 0.000 claims abstract description 19
- 239000007924 injection Substances 0.000 claims abstract description 19
- 238000002474 experimental method Methods 0.000 claims abstract description 6
- 229920001296 polysiloxane Polymers 0.000 claims description 18
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 17
- 239000000741 silica gel Substances 0.000 claims description 17
- 229910002027 silica gel Inorganic materials 0.000 claims description 17
- 239000007787 solid Substances 0.000 claims description 3
- 230000008961 swelling Effects 0.000 claims 1
- 230000006378 damage Effects 0.000 abstract description 17
- 238000010276 construction Methods 0.000 abstract description 11
- 238000010171 animal model Methods 0.000 abstract description 10
- 210000001525 retina Anatomy 0.000 abstract description 8
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 6
- 238000011160 research Methods 0.000 abstract description 3
- 210000003994 retinal ganglion cell Anatomy 0.000 abstract 1
- 208000027418 Wounds and injury Diseases 0.000 description 9
- 208000014674 injury Diseases 0.000 description 8
- 241000700159 Rattus Species 0.000 description 7
- 239000002184 metal Substances 0.000 description 6
- 230000006835 compression Effects 0.000 description 5
- 238000007906 compression Methods 0.000 description 5
- 230000007547 defect Effects 0.000 description 5
- 210000003205 muscle Anatomy 0.000 description 4
- 210000002592 gangliocyte Anatomy 0.000 description 3
- 210000003128 head Anatomy 0.000 description 3
- 230000002792 vascular Effects 0.000 description 3
- 241000700198 Cavia Species 0.000 description 2
- 241000282693 Cercopithecidae Species 0.000 description 2
- 241000699670 Mus sp. Species 0.000 description 2
- 210000000795 conjunctiva Anatomy 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000011587 new zealand white rabbit Methods 0.000 description 2
- 210000001927 retinal artery Anatomy 0.000 description 2
- 210000001957 retinal vein Anatomy 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 206010002329 Aneurysm Diseases 0.000 description 1
- 208000003098 Ganglion Cysts Diseases 0.000 description 1
- 208000005400 Synovial Cyst Diseases 0.000 description 1
- 210000000577 adipose tissue Anatomy 0.000 description 1
- 210000004712 air sac Anatomy 0.000 description 1
- 230000008081 blood perfusion Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 208000030533 eye disease Diseases 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 208000028867 ischemia Diseases 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 230000002207 retinal effect Effects 0.000 description 1
- 238000009958 sewing Methods 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
- 238000011269 treatment regimen Methods 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D1/00—Surgical instruments for veterinary use
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Surgery (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Instructional Devices (AREA)
Abstract
The invention belongs to the technical field of biomedical experiments, relates to a construction method of an animal model, and particularly relates to a method and a device for constructing an optic nerve injury model. The invention utilizes a specific device to construct an optic nerve injury model, which comprises that a pressurizing ring of the device is sleeved on the optic nerve at the back of a ball; aligning and knotting the suture lines in the threading holes, and closing the circular ring; pushing the piston of the air injection cylinder to inject air into the pressurizing ring, and fixing the piston after the air injection is finished; and after pressurization is finished, retracting the piston, sucking out air in the pressurization ring, releasing the suture, and taking the pressurization ring to construct an optic nerve injury model. The model of the invention ensures that the damage degrees of retinal ganglion cells at different parts of the retina are close to be consistent, and reduces the damage to the arteriovenous vessel wall of the retina. The method has simple and easy working procedures, and is easy to popularize in relevant researches on optic nerve injury diseases as proved by experiments.
Description
Technical Field
The invention belongs to the technical field of biomedical experiments, relates to a construction method of an animal model, and particularly relates to a method and a device for constructing an optic nerve injury model.
Background
Clinical practice shows that Traumatic Optic Neuropathy (TON) is an irreversible blinding eye disease, and no good treatment method exists at present, so that a good optic nerve injury animal model is established, and the method has important significance for mechanism research and treatment strategy exploration of optic nerve injury diseases. At present, a plurality of models for simulating clinical optic nerve injury diseases are available, such as optic nerve transection injury, optic nerve line injury, optic nerve ball injury, optic nerve clamp injury and other animal models, wherein the optic nerve clamp injury model is most widely applied; the modeling method of the optic nerve clamping injury model is that clamping instruments (including vascular clamps, aneurysm clamps, optic nerve clamps and the like) with the head width of about 1-1.5mm are adopted to clamp optic nerves (fixed clamping force) for several seconds to several minutes (adjusted according to experiment requirements) at a position of about 2-3mm behind an experimental animal ball, so that optic nerve injury is caused; however, practice has shown that the optic nerve clamp injury model has obvious defects: firstly, the forceps holder blades of the apparatus used for molding apply force to the optic nerve from two directions, and the pressures generated at the far and near ends are different, which easily causes great difference in the damage degree of ganglion cells (RGCs) at different parts of retina; secondly, when the hard metal blades clamp optic nerves, right-angle cutting force can be generated on the central arteriovenous vessel wall of the retina, so that the damage of the vessel wall is easily caused to influence the retinal blood perfusion; said defects would make the results of the studies obtained from this animal model unreliable.
Based on the current research situation of the prior art, the inventor of the application intends to provide a new method for constructing an animal model, in particular to a method and a device for constructing an optic nerve injury model.
Disclosure of Invention
The invention aims to provide a novel method for constructing an animal model, in particular to a method and a device for constructing an optic nerve injury model, aiming at overcoming the problems in the prior art and overcoming the defects that the optic nerve is stressed unevenly, the arteriovenous in the center of the retina is easy to damage and the like in the existing optic nerve clamp injury model.
The invention provides a novel method for constructing an optic nerve injury model, which utilizes a specific construction device to complete model construction.
The construction device comprises a pressurizing ring (1) for pressurizing optic nerves, a closed hollow silicone tube (6) connected with the pressurizing ring (1), and an air injection cylinder (7) connected with the silicone tube (6); the compression ring (1) is divided into a compression ring inner layer (3) and a compression ring outer layer (2); the outer layer (2) of the pressurizing ring is provided with a threading hole (5) at the head of the ring; the threading hole is internally provided with a thread (4).
The construction method comprises the following steps:
s1: sleeving the optic nerve by the pressurizing ring (1) at a position 1mm behind the ball;
s2: the suture (4) positioned in the threading hole (5) is aligned and knotted, and the circular ring is closed;
s3: the piston (8) of the air injection cylinder (7) is pushed at a constant speed to inject air into the inner layer (3) of the pressurizing ring, and after the air injection is finished, the piston (8) is fixed for a plurality of seconds to a plurality of minutes, which are different (adjusted according to experiment requirements);
s4: after the pressurization is finished, the piston (8) is retracted at a constant speed, air in the inner layer (3) of the pressurization ring is sucked out, the suture (4) is loosened, and the intraorbital pressurization ring (1) is taken out.
In the invention, the inner layer (3) of the pressurizing ring of the device is C-shaped saccular silica gel which is inflated or inflated after being filled with liquid and is communicated with a hollow silica gel tube (6);
in the invention, the outer layer (2) of the pressurizing ring of the device is non-expansion C-shaped solid silica gel which is not communicated with a hollow silica gel tube (6);
in the invention, the hollow silicone tube (6) of the device is a non-expansion hollow silicone pipeline, one end of the hollow silicone tube is communicated with the inner layer (3) of the pressurizing ring, and the other end of the hollow silicone tube is connected with the opening of the gas injection tube (7).
In the invention, an air injection cylinder (7) of the device is pre-stored with a certain volume of air and communicated with a silicone tube (6).
In the invention, the pressure ring (1) of the device is made in a series of different size ranges: in the embodiment of the invention, different size ranges of the pressurizing ring (1) can be adopted according to different experimental animals; such as:
rat: the diameter of the inner diameter of the pressure ring is 0.5-2mm, the width of the pressure ring is 1-5mm, and the thickness of the pressure ring is 2-2.5 mm;
mice: the diameter of the inner diameter of the pressure ring is 0.1-1mm, the width of the pressure ring is 0.1-1mm, and the thickness of the pressure ring is 0.1-1 mm;
guinea pigs: the diameter of the inner diameter of the pressure ring is 0.5-2mm, the width of the pressure ring is 1-6mm, and the thickness of the pressure ring is 0.5-2 mm;
new Zealand white rabbits: the diameter of the inner diameter of the pressure ring is 0.5-8mm, the width of the pressure ring is 0.5-10mm, and the thickness of the pressure ring is 0.5-4 mm;
monkey: the diameter of the inner diameter of the pressure ring is 1-10mm, the width of the pressure ring is 1-6mm, and the thickness of the pressure ring is 0.5-5 mm.
The device can be used for constructing the optic nerve injury model for different experimental animals such as experimental rats, experimental mice, experimental guinea pigs, experimental New Zealand white rabbits and experimental monkeys, and the device and the method have the advantages that through practice, the device and the method are as follows:
1. by using the device, the optic nerve can be pressed for 360 degrees, and the air pressure in the micro air bag can be transmitted to all directions evenly, so that the defect that the far-end pressure and the near-end pressure of the metal blade are unequal is overcome, and the damage degrees of the RGCs at different parts of the retina are close to be consistent.
2. By using the device, the metal blades of the traditional forceps holding instrument are replaced by the inflatable silica gel air bag with low hardness, so that the contact state of the pressure application part of the instrument and the clamped retinal artery and vein blood vessels is effectively changed; the micro air bag has low hardness and softness, is arc-shaped after being inflated, so that the vessel wall is in arc-shaped contact with the air bag, and the phenomenon that the edge of the traditional metal blade is straight can be reduced
The cutting force to the vessel wall at the angle reduces the damage to the vessel wall.
Drawings
FIG. 1 is a schematic structural view of a construction apparatus of the present invention;
FIG. 2 is a schematic front view of a compression ring in the build apparatus of the present invention;
FIG. 3 is a schematic side view of a compression ring in the build apparatus of the present invention;
wherein, 1, a pressure ring; 2, outer layer of the pressurizing ring; 3, a pressurizing ring inner layer; 4, sewing; 5, threading holes; 6, a silicone tube; 7, inflating the cylinder; 8, a piston; 9, the width of the pressure ring; 10, pressing ring thickness; 11, inner diameter of the press ring.
FIG. 4 is a schematic representation of a construction apparatus of the present invention.
Detailed Description
The present invention will be described in detail below with reference to the accompanying drawings and examples.
Example 1 construction of Experimental model for testing rat optic nerve injury diseases
As shown in figures 1-4, the construction device is used for constructing an experimental rat optic nerve injury disease model. The construction device comprises three parts: a pressurizing ring 1 for extruding optic nerve, a closed hollow silica gel tube 6 connected with the pressurizing area, and an air injection tube 7 connected with the silica gel tube. The pressurizing ring 1 is divided into an inner layer 3 and an outer layer 2; the inner layer 3 of the pressurizing ring is C-shaped saccular silica gel which is inflated or inflated after being filled with liquid and is communicated with the hollow silica gel tube 6; the outer layer 2 of the pressurizing ring is made of non-expansive solid C-shaped silica gel, and the head part of the pressurizing ring is provided with a threading hole 5 for placing a suture 4 and closing the circular ring. The silicone tube 6 is a hollow non-expansion silicone tube, one end of the silicone tube is communicated with the inner layer 3 of the pressurizing ring, and the other end of the silicone tube is connected with the gas injection cylinder 7. The air injection cylinder 7 is internally pre-stored with a certain volume of air and used for pushing and injecting the inner layer 3 of the pressurizing ring, so that the saccular silica gel expands to extrude the optic nerve.
The experimental rat optic nerve injury disease model is constructed according to the following steps:
s1: the optic nerve is sleeved by the pressurizing ring 1 at a position 1mm behind the ball;
s2: the suture 4 preset on the outer layer 2 of the pressurizing ring is aligned and knotted, and the circular ring is closed;
s3: the piston 8 of the air injection cylinder 7 is pushed at a constant speed to inject air into the inner layer 3 of the pressurizing ring, and after the air injection is finished, the piston 8 is kept fixed for 15 seconds;
s4: and retracting the piston 8 at a constant speed, sucking out air in the expanded air bag, releasing the suture 4 and taking out the intraorbital pressurizing ring 1.
Further, the pressing ring width 9 is 3 mm.
Further, the pressing ring has a thickness 10 of 1.5 mm.
Further, the inner diameter 11 of the pressing ring is 1.5 mm.
Further, the air volume reserved in the air injection cylinder 7 is 2 ml.
More specifically, the following method is adopted:
the experimental rat is characterized in that a right eye is taken as an experimental eye, a nasal bulbar conjunctiva is cut off at the edge of a conjunctiva of a corner, the upper rectus muscle and the outer rectus muscle of the rat are separated, the optic nerve is separated and exposed bluntly backwards, part of intraorbital adipose tissues are removed carefully to enlarge the volume of an orbit, a pressurizing ring is sleeved on the optic nerve by bypassing the lower rectus muscle and the inner rectus muscle after the ball, and then stitches at a threading hole are knotted in pairs so as to ensure that the closed state of the pressurizing ring is maintained when an air sac expands, then, an air injection cylinder piston is pushed at a constant speed, air is injected into the inner layer of the pressurizing ring through a silica gel tube, so that the silica gel at the inner layer of the pressurizing ring expands to pressurize the optic nerve at 360 degrees, and the piston is fixed for 15s after the air is injected, so that the optic nerve is in a stable pressure state. After the pressurization is finished, the piston is retracted at a constant speed, the air in the pressurization ring is completely sucked out, the suture is loosened, the intraorbital pressurization ring is taken out, finally, the wound is closed, and the model is successfully used for observing the patient without complete ischemia of the retina under a ophthalmoscope.
The observation and test model result shows that the method and the device can apply pressure to the optic nerve at 360 degrees, and the gas pressure in the micro air bag can be transmitted to each party equally, so that the defect that the far-end pressure and the near-end pressure of the metal blade are unequal can be overcome, and the damage degrees of the RGCs at different parts of the retina are close to be consistent; the contact state of the instrument pressing part and the clamped retinal artery and vein blood vessels can be effectively changed by using the device; can reduce the cutting force to the vascular wall when traditional metal blade edge is the right angle, alleviate the damage to the vascular wall.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are, therefore, to be considered as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Claims (7)
1. The device for constructing the optic nerve injury model is characterized by comprising a pressurizing ring (1) for pressurizing optic nerves, a closed hollow silicone tube (6) connected with the pressurizing ring (1), and an air injection cylinder (7) connected with the silicone tube (6), wherein the pressurizing ring (1) is divided into a pressurizing ring inner layer (3) and a pressurizing ring outer layer (2); the outer layer (2) of the pressurizing ring is provided with a threading hole (5) at the head of the ring; the threading hole is internally provided with a thread (4).
2. A method of constructing an optic nerve injury model, characterized by constructing the optic nerve injury model using the constructing apparatus of claim 1; which comprises the following steps:
s1: sleeving the optic nerve by the pressurizing ring (1) at a position 1mm behind the ball;
s2: the suture (4) positioned in the threading hole (5) is aligned and knotted, and the circular ring is closed;
s3: the piston (8) of the air injection cylinder (7) is pushed at a constant speed to inject air into the inner layer (3) of the pressurizing ring, and after the air injection is finished, the fixed piston (8) is adjusted for several seconds to several minutes according to the experiment requirement;
s4: after the pressurization is finished, the piston (8) is retracted at a constant speed, air in the inner layer (3) of the pressurization ring is sucked out, the suture (4) is loosened, and the intraorbital pressurization ring (1) is taken out.
3. The apparatus for constructing an optic nerve injury model according to claim 1, wherein the inner layer (3) of the pressurizing ring of the apparatus is a "C" shaped saccular silica gel which is inflated or inflated after being inflated, and is communicated with the hollow silica gel tube (6).
4. The apparatus for constructing an optic nerve injury model according to claim 1, wherein the outer layer (2) of the pressure ring of the apparatus is non-swelling "C" -shaped solid silica gel, which is not communicated with the hollow silica gel tube (6).
5. The apparatus for constructing an optic nerve injury model according to claim 1, wherein the hollow silicone tube (6) is a non-inflatable hollow silicone tube, and one end of the hollow silicone tube (6) is connected to the inner layer (3) of the pressurization ring and the other end is connected to the opening of the gas injection tube (7).
6. The apparatus for constructing an optic nerve injury model according to claim 1, wherein the apparatus for constructing is characterized in that a certain volume of air is pre-stored in the air injection cylinder (7) of the apparatus for constructing, and the apparatus is communicated with the silicone tube (6).
7. An apparatus for constructing an optic nerve injury model according to claim 1, wherein the size of the pressing ring (1) of the apparatus is in the range of:
the diameter of the inner diameter of the pressure ring is 0.5-2mm, the width of the pressure ring is 1-5mm, and the thickness of the pressure ring is 2-2.5 mm;
or the diameter of the inner diameter of the pressure ring is 0.1-1mm, the width of the pressure ring is 0.1-1mm, and the thickness of the pressure ring is 0.1-1 mm;
or the diameter of the inner diameter of the pressure ring is 0.5-2mm, the width of the pressure ring is 1-6mm, and the thickness of the pressure ring is 0.5-2 mm;
or the diameter of the inner diameter of the pressure ring is 0.5-8mm, the width of the pressure ring is 0.5-10mm, and the thickness of the pressure ring is 0.5-4 mm;
or the diameter of the inner diameter of the pressure ring is 1-10mm, the width of the pressure ring is 1-6mm, and the thickness of the pressure ring is 0.5-5 mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110024516.XA CN114732560A (en) | 2021-01-08 | 2021-01-08 | Method and device for constructing optic nerve injury model |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110024516.XA CN114732560A (en) | 2021-01-08 | 2021-01-08 | Method and device for constructing optic nerve injury model |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114732560A true CN114732560A (en) | 2022-07-12 |
Family
ID=82273900
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110024516.XA Pending CN114732560A (en) | 2021-01-08 | 2021-01-08 | Method and device for constructing optic nerve injury model |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114732560A (en) |
-
2021
- 2021-01-08 CN CN202110024516.XA patent/CN114732560A/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2647259B2 (en) | Method and apparatus for performing dorsal vein ligation | |
| CN213030943U (en) | Lumen anastomosis supporting dilator | |
| CN114732560A (en) | Method and device for constructing optic nerve injury model | |
| CN107468292A (en) | Varicocele ligatures puncture outfit under a kind of laparoscope | |
| CN201200537Y (en) | Twolip paracentesis knife for anterior chamber | |
| CN111714261A (en) | Lumen anastomosis supporting dilator | |
| CN203195717U (en) | Disposable space forming device for minimally-invasive brain surgery | |
| CN109758216B (en) | Bag type compression puncture outfit and thoracic cavity gas drainage assembly | |
| RU2577449C1 (en) | Method of simulating transient retinal ischemia in rats | |
| US20170032703A1 (en) | Simulated Organ | |
| CN111166442A (en) | Visual artificial membrane rupturing device and use method thereof | |
| CN101273928A (en) | Non-invasive negative-pressure fixer in ophthalmology | |
| CN201200451Y (en) | Perforator for middle and small arterial vessels | |
| CN211325826U (en) | Model making assembly for experimental animal with focal permanent cerebral ischemia | |
| CN110680554B (en) | Experimental animal modeling component for focal permanent cerebral ischemia | |
| CN111839796B (en) | Spontaneous hypertension middle cerebral artery ischemia reperfusion model device and method | |
| CN212037697U (en) | Visual artifical rupture of membranes auxiliary device | |
| CN203539756U (en) | Puncture aspirator for serous-cavity dropsy | |
| CN113171164B (en) | Device for repairing premature rupture of fetal membrane for in-vivo in-situ biological manufacturing | |
| CN207384289U (en) | A kind of novel vascular surgery vascular anastomosis device | |
| CN214128702U (en) | Surgical forceps for general surgery department | |
| CN210749317U (en) | Injection and expansion integrated device | |
| CN214629462U (en) | Novel accurate perfusion device for experimental rat and mouse hearts | |
| CN216455156U (en) | Adjustable hepatoduodenal ligament blocker | |
| CN218356180U (en) | ESD endoscope operation lesion part stretching device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |