CN114700127A

CN114700127A - Chip

Info

Publication number: CN114700127A
Application number: CN202210632166.XA
Authority: CN
Inventors: 不公告发明人
Original assignee: To Microbial Intelligent Technology Xiamen Co ltd; Zhimei Times Biological Intelligent Technology Beijing Co ltd
Current assignee: To Microbial Intelligent Technology Xiamen Co ltd; Zhimei Times Biological Intelligent Technology Beijing Co ltd
Priority date: 2022-06-07
Filing date: 2022-06-07
Publication date: 2022-07-05
Anticipated expiration: 2042-06-07
Also published as: CN114700127B

Abstract

The invention discloses a chip, the upper end of a through hole in the middle of a main body is connected with a top cover through an openable sealing layer, a plurality of flow channels are arranged on the periphery of the through hole, two ends of each flow channel are respectively communicated with the through hole and a reaction chamber, the storage part is hollow and is provided with a bulge at the upper end, the upper end of the bulge is provided with a sample adding port, the lower part of the side wall of the storage part is provided with a plurality of liquid inlet holes, the storage part is positioned in the through hole, the top cover is blocked at the upper end of the through hole before detection, part of the outer wall of the storage part is in transition fit with the inner wall of the through hole, the liquid inlet holes can be blocked through the inner wall of the through hole, the storage part can move upwards along the through hole to push the top cover and is in a communicated state, a sample to be added into the storage part through the sample adding port in the communicated state, and the liquid inlet holes are communicated with the flow channels, and the sample to be detected and the detection reagent in the storage part can flow into the reaction chamber through the flow channel for reaction. The chip has good sealed storage conditions, high detection precision and simple operation, and is suitable for automatic detection.

Description

Chip

Technical Field

The invention relates to the technical field of nucleic acid detection, in particular to a chip.

Background

The micro-fluidic technology is also called as micro-fluidic chip technology, and can integrate basic operation units of sample preparation, reaction, separation, detection and the like in the processes of biological, chemical and medical analysis on a chip with a few square centimeters, so that a controllable fluid can penetrate through the whole system to replace various functions of a conventional chemical or biological laboratory, and the micro-fluidic chip technology has the advantages of light volume, small amount of used samples and reagents, low energy consumption, high reaction speed, capability of massively parallel processing, disposability and the like. At present, microfluidics has great development potential and wide application prospect in biomedical research (such as nucleic acid detection).

Aerosol refers to a gaseous dispersion system composed of solid or liquid particles suspended in a gaseous medium, and the density of the solid or liquid particles may be slightly different from that of the gaseous medium or may be very different from that of the gaseous medium. For infection source detection laboratories such as judicial identification, third-party detection institutions and the like, nucleic acid detection has strong specificity and high sensitivity, plays a crucial role in disease diagnosis, but is susceptible to contamination in the operation process, resulting in false positives, wherein the most common and difficult-to-eliminate contamination is aerosol contamination. Contamination of nucleic acid detection mainly occurs at the stage of analysis of nucleic acid amplification products because the nucleic acid amplification products are large in copy amount (generally 10)¹³Copies/ml), so that a very small amount of nucleic acid amplification product causes false positives.

The chip is widely applied in the field of nucleic acid detection, the conventional chip usually pre-embeds freeze-dried detection reagents into a reaction chamber of the chip, and the embedding work of the detection reagents is time-consuming and labor-consuming because a plurality of reaction chambers of the chip are generally adopted, so that the batch production of the chip is not facilitated; meanwhile, freeze-dried detection reagents are easy to deteriorate or have reduced detection capability due to moisture, oxidation and the like, the requirement on the tightness of storage conditions is high, in the nucleic acid detection reaction process, aerosol pollution is easily caused when the chip is communicated with the atmosphere, and the accuracy of the next detection result is influenced, so that the produced chip needs to be sealed, a sealing strip for sealing the chip is uncovered before the chip is used, and the chip is sealed again before the detection reaction is carried out.

Disclosure of Invention

The invention aims to provide a chip, which solves the problems in the prior art, has good sealed storage conditions, high detection precision and simple operation, and is suitable for automatic detection.

In order to achieve the purpose, the invention provides the following scheme:

the invention provides a chip, which comprises a main body, a top cover and a storage piece, wherein the middle part of the main body is provided with a through hole, the upper end of the through hole is connected with the top cover through an openable sealing layer and is sealed by the top cover, the periphery of the through hole is provided with a plurality of flow channels, one end of each flow channel is communicated with the through hole, the other end of each flow channel is respectively communicated with a reaction chamber, the reaction chambers are not communicated with the outside, the storage piece is hollow and is provided with a bulge part at the upper end, the outer diameter of the bulge part is gradually increased from top to bottom, the upper end of the bulge part is provided with a sample adding port, a detection reagent is contained in the storage piece, the lower part of the side wall of the storage piece is provided with a plurality of liquid inlet holes, the storage piece is positioned in the through hole, the top cover is blocked at the upper end of the through hole before detection, and part of the outer wall of the storage piece is in transition fit with the inner wall of the through hole, the feed liquor hole can pass through the inner wall shutoff of through-hole, it can follow to store the piece through-hole rebound to the jack-up the top cap is in the connected state, under the connected state, through the sample addition mouth can to add the sample that awaits measuring in the storage piece, just the feed liquor hole with the runner intercommunication, and can make the sample that awaits measuring in the storage piece and detect reagent warp the runner flows in extremely reaction in the reacting chamber, the main part is made for transparent material.

Preferably, the height of the storage part is equal to the height from the lower bottom surface of the main body to the lower bottom surface of the top cover, the lower bottom surface of the storage part is flush with the lower bottom surface of the main body before detection, the storage part comprises the boss, the storage part and the base which are fixedly connected in sequence, the boss and the storage part are hollow, the outer wall of the base is in transition fit with the inner wall of the through hole, an inverted round table cavity is arranged in the base, the boss, the storage part and the base are communicated with each other, and the liquid inlet hole is formed in the lower end of the side wall of the storage part;

the outer wall of the base is provided with a circle of containing groove, the containing groove is internally used for embedding a bottom sealing ring, and the outer wall of the bottom sealing ring is in transition fit with the inner wall of the through hole.

Preferably, a groove with a downward opening is formed in the lower bottom surface of the main body, and the groove is coaxial with the base; the liquid inlet device is characterized by further comprising a first pressing platform, wherein a first limiting protrusion is fixed in the middle of the first pressing platform and can enter the groove, the height of the first limiting protrusion is smaller than the depth of the groove and is equal to the vertical distance between the lower edge of the inlet of the flow channel and the lower edge of the liquid inlet hole before detection;

the middle part of the second pressing platform is fixed with a second limiting protrusion, the first limiting protrusion can enter the groove, and the height of the second limiting protrusion is equal to the depth of the groove.

Preferably, one side of recess is equipped with a spacing groove, the outer fringe of base is fixed with a gag lever post, under the gag lever post bottom surface with the base bottom surface is on same horizontal plane, keep away from on the gag lever post the one end of base can stretch into to the spacing inslot, just the width of gag lever post equals the width of spacing groove, the degree of depth of spacing groove equals the gag lever post the height with the degree of depth sum of recess, just the gag lever post is located during the spacing inslot, the runner with the feed liquor hole aligns in vertical direction.

Preferably, the solvent storage chamber has still been seted up to the periphery of through-hole, the one end in solvent storage chamber is the liquid outlet, the liquid outlet with the through-hole intercommunication, just the liquid outlet is located the runner with between the recess, the filling opening has been seted up to the upper end of main part, the filling opening can communicate chamber and external world are stored to the solvent, just filling opening department be used for to solvent storage intracavity adds the solvent that can dissolve detect reagent, filling opening department can pass through the stopper shutoff.

Preferably, the inner bottom surface of the solvent storage cavity is an inclined plane and is close to the liquid outlet, the height of the inner bottom surface of the solvent storage cavity is gradually increased, a liquid outlet sealing ring is fixed on the periphery of the liquid outlet and is used for contacting the outer wall of the storage part.

Preferably, the liquid outlet device further comprises a third pressing platform, a third limiting protrusion is fixed in the middle of the third pressing platform and can enter the groove, the height of the third limiting protrusion is smaller than the depth of the groove, and the vertical distance between the lower edge of the liquid outlet and the lower edge of the liquid inlet hole before detection is equal to that between the lower edge of the liquid outlet and the lower edge of the liquid inlet hole.

Preferably, be fixed with first sealing washer and second sealing washer on the inner wall of through-hole, first sealing washer is located the top of the entry of runner, the second sealing washer is located the top of spacing groove, and before the detection, the feed liquor hole is located second sealing washer top, first sealing washer with the second sealing washer all is used for the contact the outer wall of storage spare.

Preferably, still be equipped with the breather pipe in the main part, the upper end export of breather pipe is located on the inner wall of through-hole and can with the inside intercommunication of through-hole, the lower extreme of breather pipe with the inside intercommunication of solvent storage chamber, be fixed with the shutoff head on the outer wall of bellying, before the detection the shutoff head can be right breather pipe upper end export shutoff, the shutoff head with the vertical distance of bellying lower extreme is greater than under the feed liquor hole along with the vertical distance between the edge under the liquid outlet.

Preferably, be equipped with the cross district on the top cap, each tip in cross district extends to the outer fringe of top cap, the periphery of top cap with the inner wall upper end of through-hole is connected, upwards pushes away during the storage piece, the bellying can promote the fracture in cross district realizes opening of top cap.

Compared with the prior art, the invention has the following technical effects:

the chip provided by the invention has the advantages that the middle part of the main body is provided with the through hole, the upper end of the through hole is connected with the top cover through the openable sealing layer and is sealed by the top cover, the periphery of the through hole is provided with a plurality of flow channels, one end of each flow channel is communicated with the through hole, the other end of each flow channel is respectively communicated with the reaction chamber, the reaction chamber is not communicated with the outside, meanwhile, the arrangement of the top cover is matched to form good sealing conditions, the detection reagent can be prevented from contacting the outside for a long time before the reaction starts, the detection reagent is prevented from deteriorating due to oxidation, dampness or pollution and the like to influence the subsequent reaction with a sample to be detected, the storage part is hollow and is provided with the upper end, the outer diameter of the convex part is gradually increased from top to bottom, the convex part can be conveniently arranged to push the top cover open when the sample to be detected needs to be added, the operation is convenient, the upper end of the convex part is provided with the sample adding port, and the detection reagent is contained in the storage part, before assembly, a detection reagent is pre-stored in a storage piece, the operation efficiency during detection is improved, a plurality of liquid inlet holes are formed in the lower portion of the side wall of the storage piece, the storage piece is located in the through hole, a top cover is plugged at the upper end of the through hole before detection, part of the outer wall of the storage piece is in transition fit with the inner wall of the through hole, the liquid inlet holes can be plugged through the inner wall of the through hole, the detection result is prevented from being influenced by pollution to the detection reagent, the storage piece can move upwards along the through hole to be jacked to the top cover and be in a communication state, a sample to be detected can be added into the storage piece through the sample adding port in the communication state, the sample to be detected and the detection reagent after being mixed in the storage piece can conveniently flow into the reaction chamber through the flow channel to react by adjusting the relative position relation of the storage piece and the flow channel, in the reaction process, the mixed liquid of the sample to be detected and the detection reagent is not contacted with the outside, so that aerosol pollution is avoided, and the accuracy of the next detection result is influenced. Compared with a common chip, the chip is only required to add the detection reagent into the storage part when the detection reagent is pre-embedded, and is not required to be added into each reaction chamber respectively, so that the operation time can be greatly reduced, and the time cost is saved.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required in the embodiments will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.

FIG. 1 is a schematic structural diagram of a chip according to a first embodiment;

FIG. 2 is a schematic view of the top cover of the first embodiment when opened;

FIG. 3 is a schematic diagram of a chip provided in the first embodiment at another angle;

FIG. 4 is a schematic cross-sectional view of a chip provided in the first embodiment;

FIG. 5 is a schematic view of a storage unit according to an embodiment;

FIG. 6 is a schematic cross-sectional view of a storage member according to one embodiment;

FIG. 7 is a schematic view of the structure of the top cover in the first embodiment;

FIG. 8 is a schematic view of the storage member of the first embodiment in cooperation with a first pressing station;

FIG. 9 is an enlarged schematic view of a portion of the structure of FIG. 8;

FIG. 10 is a schematic view of the storage member of the first embodiment in cooperation with a second pressing station;

FIG. 11 is an enlarged view of a portion of the structure of FIG. 10;

FIG. 12 is a schematic cross-sectional view of a chip provided in the second embodiment;

FIG. 13 is a schematic cross-sectional view of a chip provided in the third embodiment in one state;

FIG. 14 is a schematic cross-sectional view of a chip provided in the third embodiment in another state;

FIG. 15 is a schematic cross-sectional view of a chip (plug-sealing) provided in the fourth embodiment;

FIG. 16 is a schematic sectional view of a chip (plug-open) provided in the fourth embodiment;

FIG. 17 is a schematic view of the storage member of the fourth embodiment in cooperation with a third pressing stage;

FIG. 18 is a schematic view of the storage member of the fourth embodiment in cooperation with a first pressing stage;

FIG. 19 is a schematic view of the storage member of the fourth embodiment in cooperation with a second pressing stage;

FIG. 20 is a schematic sectional view of a chip provided in example five;

FIG. 21 is a schematic sectional view of the sixth embodiment with the vent tube plugged by the plugging head;

FIG. 22 is a schematic sectional view showing the breather pipe of the sixth embodiment in communication with the outside;

in the figure: 100. a chip; 1. a main body; 2. a top cover; 3. a storage member; 4. a groove; 5. a flow channel; 6. a reaction chamber; 7. a liquid inlet hole; 8. a sample addition port; 9. a boss portion; 10. a storage section; 11. a base; 12. a bottom seal ring; 13. a first pressing stage; 14. a first limit projection; 15. a second pressing stage; 16. a second limit projection; 17. a through hole; 18. a limiting groove; 19. a limiting rod; 20. a solvent storage chamber; 21. a liquid outlet; 22. a plug; 23. a liquid filling port; 24. a third pressing stage; 25. a third limit projection; 26. a first seal ring; 27. a second seal ring; 28. a breather pipe; 29. and (6) plugging the plug.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The invention aims to provide a chip, which solves the technical problems of poor sealing performance and low detection precision of the conventional device for detecting nucleic acid.

In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in further detail below.

Example one

As shown in FIGS. 1-11, the present embodiment provides a chip 100, which is mainly used for detecting nucleic acid, and comprises a main body 1, a top cover 2 and a storage member 3, wherein a through hole 17 is formed in the middle of the main body 1, the upper end of the through hole 17 is connected to the top cover 2 through an openable sealing layer and is sealed by the top cover 2, a plurality of flow channels 5 are formed in the periphery of the through hole 17, one end of each flow channel 5 is connected to the through hole 17, the other end of each flow channel 5 is respectively connected to a reaction chamber 6, the reaction chamber 6 is not connected to the outside, and a good sealing condition is formed by matching with the arrangement of the top cover 2, so that the detection reagent can be prevented from contacting the outside for a long time before the reaction starts, the detection reagent is prevented from deteriorating due to oxidation, moisture or pollution, and affecting the subsequent reaction with the sample to be detected, a protrusion 9 is formed in the hollow interior of the storage member 3, and the upper end of the protrusion 9 is gradually increased in outer diameter from top to bottom, the arrangement of the convex part 9 can facilitate the top cover 2 to be opened when a sample to be detected needs to be added, the operation is convenient, the upper end of the convex part 9 is provided with the sample adding port 8, the storage part 3 is internally provided with a detection reagent, the detection reagent is pre-stored in the storage part 3 before the detection, the operation efficiency during the detection is improved, the lower part of the side wall of the storage part 3 is provided with a plurality of liquid inlet holes 7, the storage part 3 is positioned in the through hole 17, the top cover 2 is blocked at the upper end of the through hole 17 before the detection, part of the outer wall of the storage part 3 is in transition fit with the inner wall of the through hole 17, the liquid inlet holes 7 are blocked by the inner wall of the through hole 17, the detection result is prevented from being polluted and influenced by the detection reagent, the storage part 3 can move upwards to the top cover 2 along the through hole 17 and is in a communicated state, the sample to be detected can be added into the storage part 3 through the sample adding port 8 in no matter the operation of the top cover 2, the operation of adding a sample to be detected is also carried out, the operation is convenient in the mean square, when the liquid inlet hole 7 is communicated with the flow channel 5, the sample to be detected and a detection reagent which are mixed in the storage part 3 can conveniently flow into the reaction chamber 6 through the flow channel 5 for reaction, the relative position relation between the storage part and the flow channel is adjusted, in the reaction process, the mixed liquid of the sample to be detected and the detection reagent is not contacted with the outside, so that aerosol pollution is avoided, and the accuracy of the next detection result is influenced, the main body 1 is made of transparent materials, so that the detection result is convenient to observe, in addition, the chip 100 is easy to assemble and produce, the detection reagent can be firstly placed in the storage part 3 for batch in-situ freeze drying, then the storage part 3 is combined with the main body 1, and the chip 100 is assembled. Compared with a common chip, when the chip 100 pre-embeds the detection reagent, the detection reagent is only required to be added into the storage part 3, and is not required to be added into each reaction chamber 6, so that the operation time can be greatly reduced, and the time cost can be saved.

Specifically, the height of the storage part 3 is equal to the height from the lower bottom surface of the main body 1 to the lower bottom surface of the top cover 2, before detection, the lower end of the storage part 3 is flush with the lower end of the main body 1, so that the chip 100 can be stably placed, and meanwhile, the top cover 2 can be used for plugging the sample adding port 8 at the upper end of the storage part 3, so that a detection reagent in the storage part 3 is prevented from entering a gap between the storage part 3 and the through hole 17 through the sample adding port 8 in the storage or transportation process, and the installation and positioning of the storage part 3 when the chip 100 is assembled are also facilitated, the storage part 3 comprises a bulge part 9, a storage part 10 and a base 11 which are fixedly connected in sequence, the upper end of the bulge part 9 is open, the bulge part 9 and the storage part 10 are both hollow, preferably, the bulge part 9 is in a circular truncated cone shape, so that the top cover 2 is convenient to open through the upper end of the bulge part 9, the storage part 10 and the base 11 are both cylindrical, and the inverted circular cone cavity is arranged inside the base 11, bellying 9, the inside intercommunication of storage portion 10 and base 11, and then can add the sample that awaits measuring to storing 3 inside through the upper end opening of bellying 9, the juncture of lateral wall lower extreme and the 11 upper ends of base in storage portion 10 is seted up to feed liquor hole 7, and then can be convenient for store in 3 liquid through feed liquor hole 7 inflow runner 5 in, furthermore, the outer wall of base 11 and the inner wall transition fit of through-hole 17, preferably, the lower extreme external diameter of bellying 9, the external diameter of storage portion 10 and the external diameter of base 11 are equalling in through-hole 17 internal diameter, it can fix in through-hole 17 through frictional force to store 3. However, the shape of each part in the storage member 11 in the chip provided by the present invention is not limited to the above-mentioned limitation, as long as the use requirement can be satisfied.

Be equipped with the cross district on top cap 2, each tip in cross district extends to the outer fringe of top cap 2, and the periphery of top cap 2 is connected with the inner wall upper end of through-hole 17, and the cross district is compared in other positions of top cap 2 and is thin, and then when upwards pushing away storage component 3, the bellying 9 can promote the fracture in cross district, realizes opening, convenient operation of top cap 2.

The lower bottom surface of the main body 1 is provided with a groove 4 with a downward opening, and the groove 4 is coaxial with the base 11; the chip 100 provided by this embodiment further includes a first pressing platform 13, a first limiting protrusion 14 is fixed in the middle of the first pressing platform 13, the first limiting protrusion 14 is cylindrical, and the first limiting protrusion 14 can enter the groove 4, the outer diameter of the first limiting protrusion 14 is larger than the outer diameter of the base 11 and smaller than the inner diameter of the groove 4, the height of the first limiting protrusion 14 is smaller than the depth of the groove 4 and equal to the vertical distance between the lower edge of the inlet of the flow channel 5 and the lower edge of the liquid inlet 7 before detection, so that when the chip 100 is placed on the first pressing platform 13, the storage part 3 is pushed up by the first limiting protrusion 14 until the lower end surface of the main body 1 contacts the first pressing platform 13, at this time, the inlet of the flow channel 5 is just communicated with the liquid inlet 7, and simultaneously, a solvent dissolving the detection reagent can be added into the sample addition port 8, so that the detection reagent is dissolved, and then the sample to be detected is added into the sample addition port 8, and is fully mixed, placing the chip 100 on a centrifugal device for centrifugation, so that the liquid in the storage part 3 flows into the reaction chamber 6 through the flow channel 5;

the chip 100 provided by this embodiment further includes a second pressing platform 15, a second limiting protrusion 16 is fixed in the middle of the second pressing platform 15, the second limiting protrusion 16 is cylindrical, the second limiting protrusion 16 can enter the groove 4, the outer diameter of the second limiting protrusion 16 is larger than the outer diameter of the base 11 and smaller than the inner diameter of the groove 4, the height of the second limiting protrusion 16 is equal to the depth of the groove 4, and further, when the chip 100 is placed on the second pressing platform 15, the storage member 3 is continuously pushed upwards by the second limiting protrusion 16 until the lower end surface of the main body 1 contacts the second pressing platform 15, at this time, the lower edge of the liquid inlet 7 is higher than the upper edge of the inlet of the flow channel 5, so that the flow channel 5 and the reaction chamber 6 are sealed by the outer wall of the storage member 3, aerosol pollution caused by the detection reaction is avoided, and finally, the detection reaction is performed in the chip 100 heating device, compared with the conventional chip, the combined design of the chip 100 and the pressing platforms provided by the present invention can greatly reduce the difficulty of the operation of the chip 100 The automatic system can unseal and seal the chip 100 only by transferring and pressing, so that human participation is reduced, labor cost is reduced, and the chip 100 is more favorably used in the automatic system.

Example two

As shown in fig. 12, the difference between the first embodiment and the second embodiment is that a circle of receiving groove is formed on the outer wall of the base 11, the receiving groove is used for embedding the bottom seal ring 12, the outer diameter of the bottom seal ring 12 is equal to the outer diameter of the base 11, and the outer wall of the bottom seal ring 12 contacts with the inner wall of the through hole 17, so that the sealing effect can be improved by the bottom seal ring 12, the detection reagent in the storage member 3 is prevented from being deteriorated due to moisture and oxidation, and the bottom seal ring 12 can be made of a rubber material.

EXAMPLE III

As shown in fig. 13-14, the difference between this embodiment and the second embodiment is that a limiting groove 18 is disposed on one side of the groove 4, a limiting rod 19 is fixed on the outer edge of the base 11, the lower bottom surface of the limiting rod 19 and the lower bottom surface of the base 11 are on the same horizontal plane, one end of the limiting rod 19, which is far away from the base 11, can extend into the limiting groove 18, the width of the limiting rod 19 is equal to the width of the limiting groove 18, and the limiting rod 19 is limited in the horizontal direction by the limiting groove 18, so that the limiting rod 19 can only move back and forth in the vertical direction, and further the storage part 3 is prevented from rotating relative to the through hole 17, which affects the alignment between the flow channel 5 and the liquid inlet hole 7, the depth of the limiting groove 18 is equal to the sum of the height of the limiting rod 19 and the depth of the groove 4, and when the limiting rod 19 is located in the limiting groove 18, the flow channel 5 and the liquid inlet hole 7 are aligned in the vertical direction.

Example four

As shown in fig. 15-19, the difference between the third embodiment and the second embodiment is that a solvent storage cavity 20 is further formed at the periphery of the through hole 17, a liquid outlet 21 is formed at one end of the solvent storage cavity 20, the liquid outlet 21 is communicated with the through hole 17, one end of the solvent storage cavity 20 away from the liquid outlet 21 is not directly communicated with the outside, the liquid outlet 21 is located between the flow channel 5 and the groove 4, a solvent capable of dissolving a detection reagent is stored in the solvent storage cavity 20, a liquid filling opening 23 is formed at the upper end of the main body 1, the liquid filling opening 23 is capable of communicating the solvent storage cavity 20 with the outside, the liquid adding port 23 is used for adding a solvent capable of dissolving the detection reagent into the solvent storage cavity 20, the liquid adding port 23 can be plugged by the plug 22, when the solvent needs to be supplemented, the plug 22 is opened, and when the supplement is completed or is not needed, the filling opening 23 is plugged by the plug 22, so that the accuracy of the detection result is prevented from being influenced by the untight sealing; when the assembled chip 100 is produced, the solvent can be pre-stored in the solvent storage cavity 20, so as to reduce the operation steps when the chip 100 is used, specifically, the storage part 3 containing the detection reagent can be inserted into the through hole 17 of the main body 1, so that the lower end face of the storage part 3 is flush with the lower end face of the main body 1, at this time, the outer wall of the storage part 3 plugs the liquid outlet 21 of the solvent storage cavity 20, then the plug 22 is opened, the corresponding solvent is added into the solvent storage cavity 20 through the liquid adding opening 23, and the plug 22 is covered to seal the solvent storage cavity 20, so that the assembly of the chip 100 is completed.

The interior bottom surface that the chamber 20 was stored to the solvent is the inclined plane, and from being close to keeping away from liquid outlet 21, the height of bottom surface increases gradually in the chamber 20 was stored to the solvent, when the volume of chamber 20 was stored to design solvent, the liquid level that needs to satisfy the solvent is less than liquid outlet 21 and goes up the edge, when guaranteeing that liquid outlet 21 is opened, the solvent can flow out from liquid outlet 21 nature, the periphery of liquid outlet 21 is fixed with out the liquid sealing washer, it is used for the outer wall that the contact stored 3 to go out the liquid sealing washer, and then can make the contact between liquid outlet 21 and the 3 outer walls of storage inseparabler, improve sealed effect, the solvent flows from liquid outlet 21 department before avoiding detecting.

The chip 100 provided in this embodiment further includes a third pressing stage 24, the third pressing stage 24 is used before the first pressing stage 13, a third limiting protrusion 25 is fixed in the middle of the third pressing stage 24, the third limiting protrusion 25 is cylindrical, the third limiting protrusion 25 can enter the groove 4, the outer diameter of the third limiting protrusion 25 is larger than the outer diameter of the base 11 and smaller than the inner diameter of the groove 4, the height of the third limiting protrusion 25 is smaller than the depth of the groove 4 and equal to the vertical distance between the lower edge of the liquid outlet 21 and the lower edge of the liquid inlet 7 before detection, when the chip 100 is placed on the third pressing stage 24, the chip 100 is pressed downward, when the lower end surface of the main body 1 contacts the upper end surface of the third pressing stage 24, the liquid outlet 21 is communicated with the liquid inlet 7, so as to add the solvent in the solvent storage cavity 20 into the storage member 3 and mix with the detection reagent, and then the operations at the first pressing stage 13 and the second pressing stage 15 in the first embodiment are performed, unlike the first embodiment, the top cover 2 can be pushed open when the chip 100 is placed on the third pressing stage 24 and pressed.

EXAMPLE five

As shown in fig. 20, the difference between the present embodiment and the fourth embodiment is that a first sealing ring 26 and a second sealing ring 27 are fixed on the inner wall of the through hole 17, the first sealing ring 26 is located above the inlet of the flow channel 5, the second sealing ring 27 is located above the limiting groove 18, and before detection, the liquid inlet hole 7 is located above the second sealing ring 27, and both the first sealing ring 26 and the second sealing ring 27 are used for contacting the outer wall of the storage member 3 and can be in close contact with the outer wall of the storage member 3, so as to enhance the sealing of the inlet of the flow channel 5 and the liquid inlet hole 7, prevent aerosol pollution, ensure the sealing performance of the storage member 3 when storing detection reagents, prevent the detection reagents from deteriorating due to oxidation, moisture, and the like, and simultaneously, enhance the sealing of detection reactions performed in the chip 100, and reduce the possibility of pollution. For a chip 100 without a stopper groove 18, the second seal ring 27 may be located above the groove 4.

EXAMPLE six

As shown in fig. 21-22, the difference between this embodiment and the fifth embodiment is that a vent pipe 28 is further disposed in the main body 1, an upper end outlet of the vent pipe 28 is located on an inner wall of the through hole 17 and can communicate with the inside of the through hole 17, a lower end of the vent pipe 28 communicates with the inside of the solvent storage cavity 20, that is, the vent pipe 28 is disposed in an inclined manner, so as to prevent the solution from remaining in the vent pipe 28 after entering the vent pipe 28 during transportation, a sealing head 29 is fixed on an outer wall of the protruding portion 9, the sealing head 29 can seal the upper end outlet of the vent pipe 28 before detection, and a rubber ring is disposed at the upper end outlet of the vent pipe 28, so as to enhance the sealing effect of the sealing head 29 on the upper end outlet of the vent pipe 28, the vertical distance between the sealing head 29 and the lower end of the protruding portion 9 is greater than the vertical distance between the lower edge of the liquid inlet 7 and the lower edge of the liquid outlet 21, so as to prevent the outer wall of the storage portion 10 from sealing the vent pipe 28, in the chip 100 provided in this embodiment, the liquid level of the solvent in the solvent storage cavity 20 can be higher than the liquid outlet 21 and lower than the upper outlet of the vent pipe 28, and when the solvent needs to be led out, the upper outlet of the vent pipe 28 is opened.

The principle and the implementation mode of the present invention are explained by applying specific examples in the present specification, and the above descriptions of the examples are only used to help understanding the method and the core idea of the present invention; meanwhile, for a person skilled in the art, according to the idea of the present invention, the specific embodiments and the application range may be changed. In view of the above, this summary should not be construed as limiting the invention.

Claims

1. A chip, characterized by: the device comprises a main body, a top cover and a storage part, wherein a through hole is formed in the middle of the main body, the upper end of the through hole is connected with the top cover through an openable sealing layer and is sealed through the top cover, a plurality of flow channels are formed in the periphery of the through hole, one end of each flow channel is communicated with the through hole, the other end of each flow channel is communicated with a reaction chamber, the reaction chambers are not communicated with the outside, the storage part is hollow, a protruding part is arranged at the upper end of the storage part, the outer diameter of the protruding part is gradually increased from top to bottom, a sample adding port is formed in the upper end of the protruding part, a detection reagent is contained in the storage part, a plurality of liquid inlet holes are formed in the lower part of the side wall of the storage part, the storage part is located in the through hole, the top cover is blocked at the upper end of the through hole before detection, and part of the outer wall of the storage part is in transition fit with the inner wall of the through hole, the feed liquor hole can pass through the inner wall shutoff of through-hole, it can follow to store the piece through-hole rebound to the jack-up the top cap is in the connected state, under the connected state, through the sample addition mouth can to add the sample that awaits measuring in the storage piece, just the feed liquor hole with the runner intercommunication, and can make the sample that awaits measuring in the storage piece and detect reagent warp the runner flows in extremely reaction in the reacting chamber, the main part is made for transparent material.

2. The chip of claim 1, wherein: the height of the storage part is equal to the height from the lower bottom surface of the main body to the lower bottom surface of the top cover, the lower bottom surface of the storage part is flush with the lower bottom surface of the main body before detection, the storage part comprises a protruding part, a storage part and a base which are fixedly connected in sequence, the protruding part and the storage part are hollow, the outer wall of the base is in transition fit with the inner wall of the through hole, an inverted round platform chamber is arranged in the base, the protruding part, the storage part and the base are communicated with each other, and the liquid inlet hole is formed in the lower end of the side wall of the storage part;

the outer wall of the base is provided with a circle of containing groove, a bottom sealing ring is embedded in the containing groove, and the outer wall of the bottom sealing ring is in transition fit with the inner wall of the through hole.

3. The chip of claim 2, wherein: the lower bottom surface of the main body is provided with a groove with a downward opening, and the groove is coaxial with the base; the liquid inlet device is characterized by further comprising a first pressing platform, wherein a first limiting protrusion is fixed in the middle of the first pressing platform and can enter the groove, the height of the first limiting protrusion is smaller than the depth of the groove and is equal to the vertical distance between the lower edge of the inlet of the flow channel and the lower edge of the liquid inlet hole before detection;

the middle part of the second pressing platform is fixed with a second limiting protrusion which can enter the groove, and the height of the second limiting protrusion is equal to the depth of the groove.

4. The chip of claim 3, wherein: one side of recess is equipped with a spacing groove, the outer fringe of base is fixed with a gag lever post, under the gag lever post the bottom surface with the bottom surface is on same horizontal plane under the base, keep away from on the gag lever post the one end of base can stretch into to the spacing inslot, just the width of gag lever post equals the width of spacing groove, the degree of depth of spacing groove equals the height of gag lever post with the degree of depth sum of recess, just the gag lever post is located during the spacing inslot, the runner with the feed liquor hole aligns in vertical direction.

5. The chip of claim 4, wherein: the periphery of through-hole has still been seted up solvent and has been stored the chamber, the one end in solvent storage chamber is the liquid outlet, the liquid outlet with the through-hole intercommunication, just the liquid outlet is located the runner with between the recess, the filling opening has been seted up to the upper end of main part, the filling opening can communicate chamber and external are stored to solvent, just filling opening department be used for to solvent storage intracavity adds the solvent that can dissolve detect reagent, filling opening department can pass through the stopper shutoff.

6. The chip of claim 5, wherein: the inner bottom surface of the solvent storage cavity is an inclined surface and is close to the liquid outlet, the height of the inner bottom surface of the solvent storage cavity is gradually increased, a liquid outlet sealing ring is fixed on the periphery of the liquid outlet and is used for contacting the outer wall of the storage part.

7. The chip of claim 5, wherein: still include the third and press the platform, the middle part that the platform was pressed to the third is fixed with the spacing suddenly of third, the spacing suddenly of third can get into in the recess, the height that the spacing suddenly of third is less than the degree of depth of recess, and equal before the detection the liquid outlet down along with the vertical distance between the feed liquor hole down along.

8. The chip of claim 5, wherein: be fixed with first sealing washer and second sealing washer on the inner wall of through-hole, first sealing washer is located the top of the entry of runner, the second sealing washer is located the top of spacing groove, and before the detection, the feed liquor hole is located second sealing washer top, first sealing washer with the second sealing washer all is used for the contact the outer wall of storage piece.

9. The chip of claim 8, wherein: still be equipped with the breather pipe in the main part, the upper end export of breather pipe is located on the inner wall of through-hole and can with the inside intercommunication of through-hole, the lower extreme of breather pipe with the inside intercommunication of solvent storage chamber, be fixed with the shutoff head on the outer wall of bellying, it is preceding to detect the shutoff head can be right breather pipe upper end export shutoff, the shutoff head with the vertical distance of bellying lower extreme is greater than under the feed liquor hole along with the vertical distance between the edge under the liquid outlet.

10. The chip of claim 1, wherein: be equipped with the cross district on the top cap, each tip in cross district extends to the outer fringe of top cap, the periphery of top cap with the inner wall upper end of through-hole is connected, upwards pushes away during the storage piece, the bellying can promote the fracture in cross district realizes opening of top cap.