CN114686361A

CN114686361A - Temperature raising and reducing system and PCR nucleic acid detector

Info

Publication number: CN114686361A
Application number: CN202011622537.3A
Authority: CN
Inventors: 庞琪; 彭铁刚
Original assignee: Shanghai Sige Biotechnology Co ltd
Current assignee: Shanghai Sige Biotechnology Co ltd
Priority date: 2020-12-31
Filing date: 2020-12-31
Publication date: 2022-07-01

Abstract

The invention provides a temperature rising and reducing system and a PCR nucleic acid detector, comprising: the front shell plate is provided with a drawing opening, and the rear shell plate is provided with an air exhaust hole; the temperature raising and lowering module is arranged on the shell in a drawing manner through the drawing opening and comprises a module shell, a heat dissipation module and a semiconductor thermostat, the module shell surrounds and limits a ventilation channel, a temperature raising and lowering area is formed at the top of the module shell, the heat dissipation module is located in the ventilation channel and is matched with the temperature raising and lowering area in position, the heat dissipation module comprises a plurality of heat dissipation fins which are parallel to each other, and a heat dissipation slit is formed in a space between every two adjacent heat dissipation fins; the air exhaust assembly comprises a plurality of first air exhausters and a second air exhauster, all the first air exhausters are located in the ventilation channel and arranged on the air outlet side of the heat dissipation module, and the second air exhausters are located in the shell and arranged on the rear shell plate. The invention can greatly improve the heat dissipation efficiency of the temperature raising and reducing system.

Description

Temperature raising and reducing system and PCR nucleic acid detector

Technical Field

The invention relates to a nucleic acid detection technology, in particular to a temperature rising and reducing system and a PCR nucleic acid detector.

Background

Polymerase Chain Reaction (PCR) is a molecular biology technique for amplifying and amplifying specific DNA fragments, and can be regarded as special DNA replication in vitro, and the biggest characteristic of PCR is that trace amount of DNA can be greatly increased.

The real-time fluorescent quantitative PCR technology is a method of adding fluorescent groups into a PCR reaction system, utilizing fluorescent signal accumulation to monitor the whole PCR process in real time, and finally carrying out quantitative analysis on an unknown template through a standard curve. The real-time fluorescence quantitative PCR technology effectively solves the limitation that the traditional quantification only can be used for end point detection, realizes the detection of the intensity of a fluorescence signal once in each cycle, records the intensity in software, and obtains a quantitative result according to a standard curve by calculating the Ct value of each sample. Where, the Ct value (Cycle threshold) refers to the number of cycles that the fluorescence signal in each reaction tube has undergone when it reaches a set threshold.

The PCR instrument generally includes an optical excitation unit and a fluorescence collection unit, wherein during the detection process, the optical excitation unit generates excitation light, and directly irradiates the sample through an LED lens, the fluorescence signal generated after the sample is excited reaches the fluorescence collection unit (e.g. a CCD camera) through an optical filter and a lens, and the software captures the image for processing and analysis. Currently, excitation of the sample is generally by thermoelectric heating, by a rotating platform based on convective heating, or by an embedded resistive heater, and requires a reaction liquid volume of only a few microliters. However, the temperature of the heated liquid cannot be increased or decreased at an extremely high speed, which brings inconvenience to detection.

Therefore, a PCR nucleic acid detecting apparatus which can easily detect a PCR nucleic acid satisfying a rapid temperature rise and fall is required.

In addition, the prior patent (CN112063522A) discloses a novel single-chamber single-channel temperature-controlled hot air heating module for PCR amplification instrument, comprising: the top and the bottom of the hot air bin are both open, the left side part and the right side part of the hot air bin are respectively provided with a plurality of vent holes, and the front part of the hot air bin is provided with a reaction plate inlet; the hot air guide plates are rectangular, corner gaps are formed in four vertex angles, two hot air guide plates are arranged, and the two hot air guide plates are respectively positioned on the left side and the right side in the hot air bin; the two air heating mechanisms are respectively positioned at the left side and the right side of the hot air bin. However, the structure of the module is complicated.

Disclosure of Invention

In view of the above-mentioned shortcomings of the prior art, the present invention provides a temperature raising and lowering system and a PCR nucleic acid detecting instrument, which can quickly and effectively dissipate the heat generated by a semiconductor temperature regulator, thereby greatly improving the heat dissipation efficiency of the temperature raising and lowering system.

In order to solve the above technical problem, the present invention provides a temperature raising and lowering system, including:

the casing comprises a front casing plate and a rear casing plate along the front-back direction of the casing, wherein the front casing plate is provided with a drawing opening, and the rear casing plate is provided with an air exhaust hole;

the temperature raising and lowering module is arranged on the shell in a drawing mode through the drawing opening and comprises a module shell, a heat dissipation module and a semiconductor temperature regulator, the module shell surrounds and limits a ventilation channel, the through direction of the ventilation channel is parallel to the drawing direction of the temperature raising and lowering module, a temperature raising and lowering area for arranging the semiconductor temperature regulator is formed in the top of the module shell, the heat dissipation module is located in the ventilation channel and matched with the temperature raising and lowering area in position, the heat dissipation module comprises a plurality of heat dissipation fins which are parallel to each other, a heat dissipation slit is formed by the space between every two adjacent heat dissipation fins, and the through direction of the heat dissipation slit is parallel to the drawing direction of the temperature raising and lowering module;

the air exhaust assembly comprises a plurality of first air exhausters and a second air exhauster, all the first air exhausters are located in the ventilation channel and arranged on the air outlet side of the heat dissipation module, and the second air exhausters are located in the shell and arranged on the rear shell plate.

Preferably, the temperature raising and reducing system further comprises an air inlet plate, and the air inlet plate is arranged at the air inlet of the module shell.

Preferably, the air inlet plate comprises a curved plate matched with the drawing opening, and a plurality of air inlet circular holes are uniformly distributed in the curved plate.

Preferably, all the first air dischargers are arranged in a straight line in a left-right direction of the cabinet.

Preferably, the first exhaust blower is smaller in size than the second exhaust blower.

Preferably, the first exhaust fan and the second exhaust fan are both exhaust fans.

Preferably, the module case includes an upper module case and a lower module case, the upper module case is in a flat plate shape, and the lower module case is in a U shape.

Preferably, the module shell is provided with a heat conducting plate on the inner peripheral wall, and the heat conducting plate is matched with the temperature rising and reducing area.

Preferably, a mounting slit for arranging the semiconductor thermostat is formed between the heat conductive plate and the heat dissipation module.

The invention also provides a PCR nucleic acid detector which comprises the temperature rising and lowering system.

As described above, the temperature raising and lowering system and the PCR nucleic acid detecting apparatus according to the present invention have the following advantageous effects: the heat dissipation module is positioned in the ventilation channel and matched with the temperature rising and reducing area, the heat dissipation module comprises a plurality of heat dissipation fins which are parallel to each other, and a space between every two adjacent heat dissipation fins forms a heat dissipation slit, so that heat generated by the semiconductor temperature regulator can be quickly and effectively conducted to the heat dissipation fins. Meanwhile, the air exhaust assembly comprises a plurality of first air exhausters and a second air exhauster, all the first air exhausters are located in the ventilation channel and are arranged on the air outlet side of the heat dissipation module, the second air exhausters are located in the shell and are arranged on the rear shell plate, and therefore the air in the heat dissipation slit can be rapidly exhausted to the shell after flowing through the first air exhausters and the second air exhausters, and therefore the heat dissipation efficiency of the heating and cooling system is greatly improved. Therefore, the temperature raising and reducing system and the PCR nucleic acid detector can quickly and effectively dissipate heat generated by the semiconductor temperature regulator, and greatly improve the heat dissipation efficiency of the temperature raising and reducing system.

Drawings

FIG. 1 is a first schematic view of a PCR nucleic acid detecting apparatus according to the present invention;

FIG. 2 is a second schematic view of the PCR nucleic acid detecting apparatus according to the present invention;

FIG. 3 is a first schematic view of the temperature raising/lowering module;

FIG. 4 is an enlarged view of portion A of FIG. 3;

FIG. 5 is a second schematic view of the temperature raising/lowering module;

FIG. 6 is a schematic view of a heat dissipation module and a first exhaust blower;

FIG. 7 is a drawing diagram showing the state of pulling of the PCR nucleic acid detecting apparatus of the present invention.

Description of the element reference numerals

1 case

11 front shell plate

111 drawing opening

12 rear shell plate

121 air exhaust hole

2 temperature raising and lowering module

21 module housing

211 connecting edge

212 upper module shell

213 lower module shell

22 heat radiation module

221 heat dissipation fin

222 heat dissipation slit

23 Ventilation channel

24 heating and cooling area

25 semiconductor temperature regulator

3 subassembly of airing exhaust

31 first air exhauster

4 air inlet plate

41 bent plate

42 air inlet round hole

Detailed Description

The following description of the embodiments of the present invention is provided for illustrative purposes, and other advantages and effects of the present invention will become apparent to those skilled in the art from the present disclosure.

It should be understood that the structures, ratios, sizes, etc. shown in the drawings are only used for matching the disclosure of the present disclosure to be understood and read by those skilled in the art, and are not used to limit the practical limitations of the present disclosure, so that the modifications, ratios, and sizes of any structures or changes of the ratio or adjustments of the sizes should still fall within the scope of the disclosure of the present disclosure without affecting the efficacy and the achievable purpose of the present disclosure. In addition, the terms "upper", "lower", "left", "right", "middle" and "one" used in the present specification are used for clarity of description, and are not intended to limit the scope of the present invention, and the relative relationship between the terms and the terms may be changed or adjusted without substantial change in the technical content.

As shown in fig. 1 to 7, the present invention provides a PCR nucleic acid detecting apparatus, which includes a housing 1, and the following components disposed in the housing 1: the temperature rising and falling module 2, a temperature rising and falling area 24 in the temperature rising and falling module 2 forms an objective table, and the objective table is used for placing a PCR chip to be tested; the imaging detection assembly is arranged above the objective table and comprises a yellow light assembly, a camera assembly and a blue light assembly, the yellow light assembly and the blue light assembly are arranged on two sides of the camera assembly, the camera focus of the camera assembly is located on the objective table, and emergent light spots of the yellow light assembly and the blue light assembly are located on the objective table.

The PCR nucleic acid detector adopts the semiconductor temperature regulator 25 in the temperature rising and falling module 2 to heat the PCR chip, the temperature rising and falling speed is high, and the temperature rising and falling of 60-90 ℃ can be completed within 2-3 s; in addition, the imaging detection assembly is arranged right above the objective table, and the arrangement is convenient for integrating the light source module (namely the yellow light assembly and the blue light assembly) with the camera shooting assembly. The sample to be detected is placed on the PCR chip, the PCR chip is placed on the objective table, the temperature rising and falling module 2 is controlled to heat and cool, the fluorescence signal generated after the sample is excited works, the imaging detection assembly works simultaneously, the camera assembly obtains the picture of the sample, and the picture is processed by subsequent software.

As shown in fig. 1 to 6, the present invention also provides a cooling and heating system for a PCR nucleic acid detecting apparatus, comprising:

the casing 1 comprises a front casing plate 11 and a rear casing plate 12 along the front-back direction of the casing 1, wherein the front casing plate 11 is provided with a drawing opening 111, and the rear casing plate 12 is provided with an exhaust hole 121;

the temperature raising and lowering module 2 is arranged on the machine shell 1 in a drawing manner through a drawing opening 111, the temperature raising and lowering module 2 comprises a module shell 21, a heat dissipation module 22 and a semiconductor thermostat 25, the module shell 21 surrounds and defines a ventilation channel 23, the through direction of the ventilation channel 23 is parallel to the drawing direction of the temperature raising and lowering module 2, a temperature raising and lowering area 24 for arranging the semiconductor thermostat 25 is formed at the top of the module shell 21, the heat dissipation module 22 is located in the ventilation channel 23 and matched with the temperature raising and lowering area 24 in position, the heat dissipation module 22 comprises a plurality of heat dissipation fins 221 which are parallel to each other, a space between every two adjacent heat dissipation fins 221 forms a heat dissipation slit 222, and the through direction of the heat dissipation slit 222 is parallel to the drawing direction of the temperature raising and lowering module 2;

the exhaust assembly 3 includes a plurality of first exhaust devices 31 (for example, three first exhaust devices 31) and a second exhaust device (not shown), all the first exhaust devices 31 are located in the ventilation channel 23 and are disposed on the air outlet side of the heat dissipation module 22, and the second exhaust device is located in the casing 1 and is disposed on the rear casing 12.

In the present invention, the temperature rising and lowering module 2 includes a semiconductor thermostat 25 (the semiconductor thermostat 25 includes a semiconductor cooler, which may be referred to as a TEC chip), and the semiconductor thermostat 25 is located in the temperature rising and lowering area 24 of the module housing 21, and can heat a to-be-detected component (such as a PCR chip), and the temperature rising and lowering rate is high, and the temperature rising and lowering can be completed within 1s by 10 ℃. Therefore, the temperature raising and lowering system of the present invention is required to quickly and efficiently dissipate heat from the semiconductor thermostat 25.

The heat dissipation principle of the temperature raising and reducing system is introduced as follows:

the heat dissipation module 22 is located in the ventilation channel 23 and is matched with the temperature rising and lowering area 24, the heat dissipation module 22 includes a plurality of heat dissipation fins 221 parallel to each other, and a space between two adjacent heat dissipation fins 221 forms a heat dissipation slit 222, so that heat generated by the semiconductor thermostat 25 can be quickly and effectively conducted to the heat dissipation fins 221. Meanwhile, the exhaust assembly 3 comprises a plurality of first exhaust devices 31 and a second exhaust device, all the first exhaust devices 31 are located in the ventilation channel 23 and are arranged on the air outlet side of the heat dissipation module 22, the second exhaust device is located in the casing 1 and is arranged on the rear shell plate 12, and by the arrangement, air in the heat dissipation slit 222 can be rapidly discharged to the casing 1 after flowing through the first exhaust devices 31 and the second exhaust devices, so that the heat dissipation efficiency of the temperature raising and reducing system is greatly improved. Therefore, the temperature raising and reducing system of the present invention can rapidly and effectively dissipate the heat generated by the semiconductor thermostat 25, and greatly improve the heat dissipation efficiency of the temperature raising and reducing system.

As shown in fig. 1 and fig. 2, in order to avoid the exposure of the heat dissipation module 22, the temperature raising and lowering system further includes an air inlet plate 4, and the air inlet plate 4 is disposed at an air inlet of the module housing 21.

Further, the air inlet plate 4 includes a curved plate 41 adapted to the drawing opening 111, and a plurality of air inlet circular holes 42 are uniformly distributed on the curved plate 41. When the temperature rising and lowering module 2 is pushed into the casing 1, the outer side wall of the air inlet plate 4 and the outer side wall of the casing 1 are spliced in a clearance mode, and therefore the attractiveness of the temperature rising and lowering system is improved.

As shown in fig. 6, all the first air blowers 31 are linearly arranged in the left and right direction of the cabinet 1 in order to uniformly discharge the air in the heat-discharging slits 222.

Since the number of the first exhaust fans 31 is greater than that of the second exhaust fans, the size of the first exhaust fans 31 is smaller than that of the second exhaust fans.

As an embodiment of the first and

second air exhausters

31 and 31 described above: the first and

second air exhausters

31 and 31 are both air exhausters.

As shown in fig. 6, in order to fix the heat dissipation module 22 in the ventilation channel 23, the module case 21 has a connection edge 211 on the inner peripheral wall thereof, to which the heat dissipation module 22 is mounted.

As shown in fig. 3, in order to facilitate the attachment and detachment of the module case 21, the module case 21 includes an upper module case 212 and a lower module case 213, the upper module case 212 has a flat plate shape, and the lower module case 213 has a U-shape. Upper module housing 212 and lower module housing 213 may be removably coupled by a coupling assembly (e.g., bolts).

In order to more effectively transfer the heat generated from the semiconductor thermostat 25 to the object to be inspected, a heat conducting plate (not shown) is provided on the inner peripheral wall of the module case 21, and the heat conducting plate is positioned to match the temperature rising and falling region 24. Further, a mounting slit for the semiconductor thermostat 25 is formed between the heat conductive plate and the heat dissipation module 22.

In conclusion, the temperature raising and reducing system and the PCR nucleic acid detector can quickly and effectively dissipate heat generated by the semiconductor temperature regulator, and greatly improve the heat dissipation efficiency of the temperature raising and reducing system. Therefore, the invention effectively overcomes various defects in the prior art and has high industrial utilization value.

The foregoing embodiments are merely illustrative of the principles and utilities of the present invention and are not intended to limit the invention. Any person skilled in the art can modify or change the above-mentioned embodiments without departing from the spirit and scope of the present invention. Accordingly, it is intended that all equivalent modifications or changes which can be made by those skilled in the art without departing from the spirit and technical spirit of the present invention be covered by the claims of the present invention.

Claims

1. A temperature raising and lowering system, comprising:

the fan comprises a shell (1), wherein the shell (1) comprises a front shell plate (11) and a rear shell plate (12) along the front-rear direction of the shell, a drawing opening (111) is formed in the front shell plate (11), and an air exhaust hole (121) is formed in the rear shell plate (12);

the temperature raising and reducing module (2), the temperature raising and reducing module (2) is arranged on the shell (1) through a drawing opening (111) in a drawing way, the temperature raising and reducing module (2) comprises a module shell (21), the semiconductor temperature regulator comprises a heat dissipation module (22) and a semiconductor temperature regulator (25), wherein a module shell (21) surrounds and defines a ventilation channel (23), the through direction of the ventilation channel (23) is parallel to the drawing direction of the temperature rise and drop module (2), a temperature rise and drop area (24) for arranging the semiconductor temperature regulator (25) is formed at the top of the module shell (21), the heat dissipation module (22) is located in the ventilation channel (23) and is matched with the temperature rise and drop area (24), the heat dissipation module (22) comprises a plurality of heat dissipation fins (221) which are parallel to each other, a space between every two adjacent heat dissipation fins (221) forms a heat dissipation slit (222), and the through direction of the heat dissipation slit (222) is parallel to the drawing direction of the temperature rise and drop module (2);

the air exhaust assembly (3) comprises a plurality of first air exhausters (31) and a second air exhauster, all the first air exhausters (31) are located in the ventilation channel (23) and arranged on the air outlet side of the heat dissipation module (22), and the second air exhauster is located in the machine shell (1) and arranged on the rear shell plate (12).

2. The warming and cooling system according to claim 1, wherein: the temperature raising and reducing system further comprises an air inlet plate (4), wherein the air inlet plate (4) is arranged at an air inlet of the module shell (21).

3. The warming and cooling system according to claim 2, wherein: the air inlet plate (4) comprises a curved plate member (41) matched with the drawing opening (111), and a plurality of air inlet circular holes (42) which are uniformly distributed are formed in the curved plate member (41).

4. The warming and cooling system according to claim 1, wherein: all the first air blowers (31) are linearly arranged along the left and right direction of the machine shell (1).

5. The warming and cooling system according to claim 1, wherein: the size of the first air exhauster (31) is smaller than that of the second air exhauster.

6. The warming and cooling system according to claim 1, wherein: the first exhaust fan (31) and the second exhaust fan are both exhaust fans.

7. The warming and cooling system according to claim 1, wherein: the module shell (21) comprises an upper module shell (212) and a lower module shell (213), the upper module shell (212) is flat, and the lower module shell (213) is U-shaped.

8. The warming and cooling system according to claim 1, wherein: the inner peripheral wall of the module shell (21) is provided with a heat conducting plate, and the heat conducting plate is matched with the temperature rising and reducing area (24).

9. The warming and cooling system according to claim 8, wherein: a mounting slit for arranging the semiconductor temperature regulator (25) is formed between the heat conducting plate and the heat dissipation module (22).

10. A PCR nucleic acid detecting instrument, characterized in that: comprising the warming and cooling system as claimed in any one of claims 1 to 9.