CN114685200A - Degradation method of peach tree waste - Google Patents
Degradation method of peach tree waste Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C9/00—Fertilisers containing urea or urea compounds
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention relates to a degradation method of peach tree waste, which comprises the following steps: crushing peach branches into peach branch powder; adding water, a carbon source and a composite degrading microbial inoculum into the peach branch powder for fermentation; the composite degrading Bacteria agent comprises Bacillus subtilis liquid, saccharomycete liquid, rhodopseudomonas palustris (Photosynthetic Bacteria), Azotobacter sp liquid, Trichoderma spp composite degrading Bacteria liquid and an adsorbent. The peach branch degradation method has the function of rapidly degrading peach branches, and tests prove that the method can reduce the lignin content of the peach branches from about 25.1 percent to below 12.7 percent and the cellulose content from about 12 percent to below 7 percent in 80-90 days.
Description
Technical Field
The invention relates to the field of ecological environment protection, in particular to a method for degrading peach tree wastes.
Background
Lignin is widely present in almost all plant-based biomass materials and is an important component of plant cell walls. The content of lignin in the nature is second to that of cellulose, the lignin is a natural high molecular compound with a complex structure, and is a polyphenol three-dimensional reticular high molecular compound formed by butting phenylpropane units through ether bonds and carbon-carbon bonds. The more 600 trillion tons of lignin are produced worldwide per year. It is a byproduct in the paper industry and the wood hydrolysis industry, and is a substance which is difficult to degrade in waste branches and leaves of crops. The pulp and paper industry separates about 1.4 million tons of cellulose from plants each year, while obtaining about 5000 million tons of lignin product.
The yield of the waste branches of crops is more than 20 hundred million tons every year in the world, and China occupies 5 hundred million tons. Taking the flat valley as a famous peach producing base as an example, the average annual production of rejected waste peach branches is about 15 million tons or more. Because the lignin has large molecular weight and complex structure and the lignification degree of peach branches is high, the natural degradation rate of the lignin is very slow, and the lignin has little influence on the regional environment.
From a very early time, scholars at home and abroad start to research lignin degradation technology, and related physical methods, chemical methods and physical and chemical methods have low degradation rate or easily cause environmental pollution.
Disclosure of Invention
The invention aims to solve the technical problems of difficult treatment and low degradation rate of lignin in the prior art.
According to the invention, the degradation method of the peach tree waste is provided, which comprises the following steps: crushing peach branches into peach branch powder; adding water, a carbon source and a composite degrading microbial inoculum into the peach branch powder for fermentation, wherein the fermentation environment temperature is 15-38 ℃, and the fermentation time is 80-90 days.
Wherein the composite degrading microbial inoculum comprises a composite degrading microbial inoculum consisting of Bacillus subtilis, microzyme (saccharomycetes), rhodopseudomonas palustris (Photosynthetic bacterial), Azotobacter sp, Trichoderma and an adsorbent, wherein in the composite degrading microbial inoculum, the Bacillus subtilis isThe strain content of the bacillus liquid is 3.60 multiplied by 109More than one per gram, the strain content of the saccharomycete liquid is 3.6 multiplied by 109More than one per gram, the bacterial strain content of the rhodopseudomonas palustris bacterial liquid is 2.40 multiplied by 109More than one gram, the strain content of the azotobacteria liquid is 1.8 multiplied by 109More than one per gram, the strain content of the trichoderma fungus liquid is 3.60 multiplied by 109More than one per gram, total bacteria number in the composite degradation bacteria agent>4.5×109/g。。
The mass of the composite degrading bacterial liquid and the adsorbent is 1:2, and the mass of the peach branch powder is as follows: water: urea: the composite degrading microbial inoculum is 4-5:3-5: 0.015: 0.005-0.01.
Wherein the fermentation mode is natural ventilation composting fermentation.
The method comprises the steps of starting from the feeding day, simultaneously recording the ambient temperature and the temperature of a stack, measuring the temperature in the morning every day, starting to turn the stack for the first time when the temperature of the stack reaches above 60 ℃, measuring the temperature and the water of the stack for the early, middle and late days every day, turning the stack once when the temperature of the stack exceeds 60 ℃, and supplementing the water of the stack to 60% when the water content of the stack is lower than 50%.
Wherein the size of the peach branch powder is 05-2.5cm, the carbon source is urea, and the adsorbent is turf or vermiculite.
Wherein the Bacillus subtilis liquid is obtained by activating Bacillus subtilis, inoculating into Bacillus subtilis culture medium, and enriching, wherein 1L Bacillus subtilis culture medium contains semen Maydis powder 30g, bean cake 35g, and NaCL 5g and the balance 1L of distilled water; wherein the enrichment condition is that air is purified, and oxygen is added to keep dissolved oxygen above 5 PPM; the fermentation temperature was 37 ℃.
Wherein, the yeast bacterial liquid is obtained by activating yeast and then inoculating the activated yeast in a yeast culture medium for enrichment, wherein 1L of the culture medium comprises 40 g of starch, 15g of agar and the balance of 1L of distilled water; the enrichment condition is that the fermentation temperature is 25-28 deg.C, pH is 4-6, air is purified, and oxygen is added to maintain dissolved oxygen above 4 PPM.
The trichoderma harzianum liquid is obtained by activating trichoderma harzianum and then inoculating the activated trichoderma harzianum liquid in a mildew culture medium for enrichment, wherein 1L of the culture medium comprises 1L of 20% potato juice, 3g of monopotassium phosphate, 1.5g of magnesium sulfate and 20g of glucose; the enrichment conditions are as follows: keeping the fermentation temperature at 28 deg.C, introducing purified air, and increasing oxygen to maintain dissolved oxygen above 5 PPM.
Wherein, the rhodopseudomonas palustris bacterial liquid is prepared by inoculating activated rhodopseudomonas palustris in a rhodopseudomonas palustris culture medium for enrichment; wherein 1L of rhodopseudomonas palustris culture medium comprises 30g of corn flour, 1g of monopotassium phosphate, 0.5g of magnesium sulfate and the balance of 1L of distilled water; the enrichment conditions are as follows: the fermentation temperature is 30 ℃, and the illumination intensity is 4000 and 5000 lux.
Wherein, the azotobacteria bacterium liquid is formed by inoculating activated azotobacteria into an azotobacteria culture medium for enrichment; wherein 1L of azotobacter culture medium contains 10g of glucose, 1g of monopotassium phosphate, 0.2g of magnesium sulfate, 0.2g of calcium sulfate and the balance of 1L of distilled water; the enrichment conditions are as follows: introducing purified air, and maintaining the fermentation temperature at 25-28 deg.C.
Experiments prove that the five strains generate synergistic interaction by enrichment and mixing under the combined action of rhodopseudomonas palustris, azotobacter and saccharomycetes by using the bacillus subtilis and the trichoderma. Tests prove that the degradation rate of the bacillus subtilis and the trichoderma in the preparation on lignin and cellulose in peach branches is greatly improved.
Compared with the prior art, the invention has the following beneficial effects:
the method for degrading the peach tree wastes has the function of quickly degrading the peach branches, and tests prove that the method can reduce the lignin content of the peach branches from about 25.1 percent to below 12.7 percent and the cellulose content from about 12 percent to below 7 percent in 80-90 days.
DETAILED DESCRIPTION OF EMBODIMENT (S) OF INVENTION
Exemplary embodiments of the present disclosure will be described in more detail below. While the following shows exemplary embodiments of the disclosure, it will be understood that the disclosure may be embodied in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the disclosure to those skilled in the art.
In the present invention, the original sources of the used strains are as follows:
bacillus subtilis purchased from Beijing Baina union of Industrial science and biotechnology research institute and deposited in Beijing biological microorganism strain collection center with the collection number BNCC 193111.
Yeast (Saccharomyces), purchased from research institute of Biotechnology, Baina, Beijing, and deposited in Beijing biological microorganism culture Collection with the deposit number BNCC 336054.
The rhodopseudomonas palustris is purchased from Beijing Baina institute of Biotechnology and is preserved in Beina biological microorganism culture Collection with the preservation number of BNCC 336448.
The azotobacteria (Azotobacter sp.) used is purchased from Beijing Baina Biotechnology research institute and is deposited in Beijing biological microorganism strain collection with the collection number BNCC 335805.
The Trichoderma (Trichoderma spp.) used was purchased from the institute of Biotechnology, Baina, Beijing and collected in the Beina biological microorganism culture Collection with the collection number BNCC 189286.
Example 1 enrichment of the Medium
1. The bacillus subtilis culture medium comprises the following components:
1L culture medium containing corn flour 30g, soybean meal 35g, and NaCL 5g and the balance 1L of distilled water.
2. The yeast culture medium comprises the following components:
the yeast culture medium comprises the following components: 1L of the culture medium contains 40 g of starch, 15g of agar and the balance of 1L of distilled water.
3. The components of the rhodopseudomonas palustris culture medium are as follows:
the rhodopseudomonas palustris culture medium comprises the following components: the 1L culture medium contains corn flour 30g, potassium dihydrogen phosphate 1g, magnesium sulfate 0.5g, and the balance 1L distilled water.
4. The nitrogen-fixing bacteria culture medium comprises the following components:
the nitrogen-fixing bacteria culture medium comprises the following components: 1L of culture medium contains 10g of glucose, 1g of monopotassium phosphate, 0.2g of magnesium sulfate, 0.2g of calcium sulfate and the balance of 1L of distilled water.
5. The components of the trichoderma culture medium are as follows:
the trichoderma culture medium comprises the following components: the 1L culture medium comprises 20% potato juice 1L, potassium dihydrogen phosphate 3g, magnesium sulfate 1.5g, and glucose 20 g.
Example 2 enrichment Process
1. The enrichment process of the bacillus subtilis comprises the following steps: and unfreezing the purchased bacillus subtilis microbial inoculum, inoculating the bacillus subtilis microbial inoculum into a screening culture medium for culture, selecting a thriving colony from the bacillus subtilis microbial inoculum, streaking and inoculating the selected colony again, and repeatedly screening for 4-5 times to obtain a well-grown colony. Selecting the same components as the screening culture medium as an enrichment culture medium, selecting a colony with good growth to be inoculated into the enrichment culture medium, and carrying out enrichment fermentation culture. The fermentation culture conditions are that air is purified, and oxygen is added to keep dissolved oxygen above 5 PPM; the fermentation temperature was 37 ℃. Obtaining the bacillus subtilis fermentation liquor.
2. The enrichment process of the yeast comprises the following steps: and unfreezing the purchased yeast agent, inoculating the thawed yeast agent into a screening culture medium for culture, selecting out a thriving colony from the culture medium for streaking and inoculating again, and repeatedly screening for 4-5 times to obtain a colony with good growth. Selecting the same components as the screening culture medium as an enrichment culture medium, selecting a colony with good growth to be inoculated into the enrichment culture medium, and carrying out enrichment fermentation culture. The enrichment fermentation conditions are as follows: fermenting at 25-28 deg.C and pH of 4-6, purifying air, and adding oxygen to maintain dissolved oxygen above 4 PPM. Obtaining the yeast fermentation liquor.
3. The enrichment process of rhodopseudomonas palustris comprises the following steps: and unfreezing the purchased rhodopseudomonas palustris microbial inoculum, inoculating the rhodopseudomonas palustris microbial inoculum into a screening culture medium for culture, selecting out a thriving colony from the rhodopseudomonas palustris microbial inoculum, streaking and inoculating the thriving colony again, and repeatedly screening for 4-5 times to obtain a well-grown colony. Selecting the same components as the screening culture medium as an enrichment culture medium, selecting a colony with good growth to be inoculated into the enrichment culture medium, and carrying out enrichment fermentation culture. The conditions of enrichment fermentation are as follows: the enrichment fermentation conditions are as follows: the fermentation temperature is 30 ℃, and the illumination intensity is 4000 and 5000 lux. Obtaining the rhodopseudomonas palustris fermentation liquor.
4. And (3) enriching nitrogen-fixing bacteria: and unfreezing the purchased azotobacter inoculum, inoculating the obtained azotobacter inoculum into a screening culture medium for culture, selecting out a growing and robust bacterial colony from the obtained azotobacter inoculum, streaking and inoculating the bacterial colony again, and repeatedly screening for 4-5 times to obtain the bacterial colony with good growth. Selecting the same components as the screening culture medium as an enrichment culture medium, selecting a colony with good growth to be inoculated into the enrichment culture medium, and carrying out enrichment fermentation culture. The conditions of enrichment fermentation are as follows: introducing purified air, and maintaining the fermentation temperature at 25-28 deg.C. Obtaining the azotobacter fermentation liquor.
5. And (3) enrichment process of trichoderma: and unfreezing the purchased trichoderma fungicide, inoculating the trichoderma fungicide into a screening culture medium for culture, selecting out a thriving colony from the trichoderma fungicide, streaking and inoculating the colony again, and repeatedly screening for 4-5 times to obtain a colony with good growth. Selecting the same components as the screening culture medium as an enrichment culture medium, selecting a colony growing well, inoculating into the enrichment culture medium, and performing enrichment fermentation culture. The enrichment fermentation conditions are as follows: maintaining the fermentation temperature at 28 deg.C, introducing purified air, and increasing oxygen to maintain dissolved oxygen above 5 PPM. Obtaining the trichoderma fermentation liquor.
Example 3 preparation of Complex degrading microbial inoculum
Respectively diluting and coating the obtained bacillus subtilis fermentation liquor, yeast fermentation liquor, rhodopseudomonas palustris fermentation liquor, azotobacter fermentation liquor and trichoderma fermentation liquor, and selecting bacillus subtilis with the weight of 3.60 multiplied by 1093.6 multiplied by 10 yeast9Rhodopseudomonas palustris 2.4X 1091.8 × 10 Azotobacter9Trichoderma reesei of 3.60X 109Adding nutrient solution to prepare 1g of composite degradation bacteria liquid.
Sieving adsorbent with 50 mesh sieve, adding into composite degrading bacteria solution at a ratio of 2:1, and making into solid composite degrading bacteria agent with total bacteria number>4.5×109(ii) in terms of/g. (the adsorbent is turf or vermiculite).
The composition of the microbial composite degradation bacterial liquid related to the invention is exemplarily shown in the form of a table below. The microbial composite degradation bacterial liquid shown in the following table does not represent all embodiments of the microbial composite degradation bacterial liquid, and the scope of the claims of the microbial composite degradation bacterial agent of the present invention is subject to the final limitation of the claims.
Table 1: 1g of microorganism composite degradation bacterial liquid composition (unit: strain)
Example 4 peach tree waste degradation method
Pulverizing collected peach branches, and sequentially pulverizing into 0.5-2.5cm peach branch powder by coarse powder machine and fine powder machine. And (4) removing dust from the peach branches, and sterilizing by using an ultrasonic sterilizer. Putting the sterilized peach branch scraps into a sterile fermentation device, adding water for swelling, supplementing urea serving as a carbon source, and finally uniformly scattering the composite degrading microbial inoculum prepared in the embodiment 3, wherein the adding ratio of the peach branch scraps, the water, the urea and the composite degrading microbial inoculum is 5:5: 0.015: 0.01. naturally ventilating and fermenting at ambient temperature of 15-38 deg.C and normal pressure. The dimensions of the fermentation stack were 10 meters long, 2.5 meters wide and 1.2 meters high. Turning over once on the third day of fermentation; turning over the pile once to twice every day on the fourth to fourteenth days of fermentation, replenishing water once on the tenth day of fermentation, turning over the pile once every day on the fifteenth to twenty-fourth days of fermentation, and replenishing water once on twenty-four days. The internal temperature range of the fermentation bar stack is 19-78 ℃. After 90 days (2019.5.19-2019.8.17), the lignin and cellulose content was again determined.
According to the determination of polysaccharide and lignin content in the national standard GB/T35818-. The results show that the lignin content of the peach branch scraps is 25.3% before degradation, and 12.4% after degradation. Before degradation, the cellulose content of the peach branch scraps is 12%, and the cellulose content after degradation is 6.1%.
Example 5 degradation method of peach tree waste
Pulverizing collected peach branches, and sequentially pulverizing into 0.5-2.5cm peach branch powder by coarse powder machine and fine powder machine. Putting the peach branch scraps into a fermentation device, adding water for swelling, supplementing urea serving as a carbon source, and finally uniformly scattering the composite degradation microbial inoculum prepared in the embodiment 3, wherein the adding ratio of the peach branch scraps, the water, the urea and the composite degradation microbial inoculum is 5:4: 0.015: 0.01. naturally ventilating and fermenting at ambient temperature of 15-38 deg.C and normal pressure. The dimensions of the fermentation stack were 10 meters long, 2.5 meters wide and 1.2 meters high. Turning over once on the third day of fermentation; turning over the pile once to twice every day on the fourth to fourteenth days of fermentation, replenishing water once on the tenth day of fermentation, turning over the pile once every day on the fifteenth to twenty-fourth days of fermentation, and replenishing water once on twenty-four days. The internal temperature range of the fermentation bar stack is 20-75 ℃. After 90 days (2019.5.19-2019.8.17), the lignin and cellulose content was again determined.
According to the determination of polysaccharide and lignin content in the national standard GB/T35818-. The results show that the lignin content of the peach branch scraps is 25.1% before degradation, and the lignin content of the peach branch scraps is 12.1% after degradation. Before degradation, the cellulose content of the peach branch scraps is 12%, and the cellulose content after degradation is 6.0%.
Example 6 degradation method of peach tree waste
Pulverizing collected peach branches, and sequentially pulverizing into 0.5-2.5cm peach branch powder by coarse powder machine and fine powder machine. Putting the peach branch scraps into a fermentation device, adding water for swelling, supplementing urea serving as a carbon source, and finally uniformly scattering the composite degradation microbial inoculum prepared in the embodiment 3, wherein the adding ratio of the peach branch scraps, the water, the urea and the composite degradation microbial inoculum is 5:3: 0.015: 0.01. naturally ventilating and fermenting at ambient temperature of 15-38 deg.C and normal pressure. The size of the fermentation stack was 12 meters long, 2.0 meters wide and 1.0 meter high. Turning over once on the third day of fermentation; turning over the pile once to twice every day on the fourth to fourteenth days of fermentation, replenishing water once on the tenth day of fermentation, turning over the pile once every day on the fifteenth to twenty-fourth days of fermentation, and replenishing water once on twenty-four days. The internal temperature range of the fermentation bar stack is 19-78 ℃. After 90 days (2019.5.19-2019.8.17), the lignin and cellulose content was again determined.
According to the determination of polysaccharide and lignin content in the national standard GB/T35818-. The results show that the lignin content of the peach branch scraps before degradation is 25.1%, and the lignin content of the peach branch scraps after degradation is 12.7%. Before degradation, the cellulose content of the peach branch scraps is 12%, and the cellulose content after degradation is 7.0%.
Example 7 peach tree waste degradation method
Pulverizing collected peach branches, and sequentially pulverizing into 0.5-2.5cm peach branch powder by coarse powder machine and fine powder machine. Putting the peach branch scraps into a fermentation device, adding water for swelling, supplementing urea serving as a carbon source, and finally uniformly scattering the composite degradation microbial inoculum prepared in the embodiment 3, wherein the adding ratio of the peach branch scraps, the water, the urea and the composite degradation microbial inoculum is 4:3: 0.015: 0.01. naturally ventilating and fermenting at ambient temperature of 15-38 deg.C and normal pressure. The size of the fermentation stack was 15 meters long, 2.8 meters wide and 1.5 meters high. Turning over once on the third day of fermentation; turning over the pile once to twice every day on the fourth to fourteenth days of fermentation, replenishing water once on the tenth day of fermentation, turning over the pile once every day on the fifteenth to twenty-fourth days of fermentation, and replenishing water once on twenty-four days. The internal temperature range of the fermentation bar stack is 20-70 ℃. After 80 days (2019.5.19-2019.8.07), the lignin and cellulose content was again determined.
According to the determination of polysaccharide and lignin content in the national standard GB/T35818-. The results showed that the lignin content of the peach branch chips before degradation was 25.0%, and the lignin content of the peach branch chips after degradation was 11.7%. Before degradation, the cellulose content of the peach branch scraps is 12.3%, and the cellulose content after degradation is 6.4%.
Comparative example 1
In the bacterial liquid prepared in the example 2, the same number of bacillus subtilis and trichoderma contained in the composite degrading bacterial agent prepared in the example 3 are selected for compounding to prepare a composite degrading bacterial agent X1. The same batch of peach branch crumbs was subjected to fermentation treatment in the same test manner as in example 4. Other fermentation conditions were kept the same and the same method was used for detection. Through detection, the lignin content of the peach branch scraps is 25.1% before degradation, and the lignin content of the peach branch scraps is 20.87% after degradation. Before degradation, the cellulose content of the peach branch scraps is 12.3%, and the cellulose content after degradation is 9.7%.
Comparative example 2
In the bacterial liquid prepared in the example 2, the same number of yeasts, rhodopseudomonas palustris and azotobacter as the composite degrading bacterial agent prepared in the example 3 are selected and compounded to prepare a composite degrading bacterial agent X2. The same amount of peach branch powder was subjected to fermentation treatment in the same test manner as in example 4. Other fermentation conditions were kept the same and the same method was used for detection. Through detection, the lignin content of the peach branch scraps is 25.1% before degradation, and the lignin content of the peach branch scraps is 22.73% after degradation. Before degradation, the cellulose content of the peach branch scraps is 11.9%, and the cellulose content after degradation is 10.5%.
Comparative example 3
The same test mode as that of example 4 was adopted without adding any composite degrading microbial inoculum, and the same amount of peach branch crumbs in the same batch were subjected to fermentation treatment. Other fermentation conditions were kept the same and the same method was used for detection. Through detection, the lignin content of the peach branch scraps is 25.0% before degradation, and the lignin content of the peach branch scraps is 22.54% after degradation. Before degradation, the cellulose content of the peach branch scraps is 11.7%, and the cellulose content after degradation is 10.7%.
Comparative example 4
Straw returning and decomposing agent produced by Guangzhou city micro-biological technology limited company is used as degrading microbial inoculum to ferment the same batch of peach branch scraps. The other fermentation conditions were kept the same as in example 4 and the same method was used for the detection. Through detection, the lignin content of the peach branch scraps is 24.8% before degradation, and the lignin content of the peach branch scraps is 21.1% after degradation. Before degradation, the cellulose content of the peach branch scraps is 12.1%, and the cellulose content after degradation is 8.8%.
Comparative example 5
All the bacterial liquids prepared in example 2 were selected and re-compounded. Keeping the number of the compounded bacillus subtilis and trichoderma to be the same as that of the compound bacillus subtilis and trichoderma in the embodiment 3, and reducing the number of the saccharomycetes, the rhodopseudomonas palustris and the azotobacter to be 0.5 multiplied by 109And (5) preparing the compound degrading microbial inoculum X3 per gram. The same amount of peach branch powder was subjected to fermentation treatment in the same test manner as in example 4. Other fermentation conditions were kept the same and the same method was used for detection. Through detection, the lignin content of the peach branch scraps is 24.9% before degradation, and the lignin content of the peach branch scraps is 20.77% after degradation. Before degradation, the cellulose content of the peach branch scraps is 12.3%, and the cellulose content after degradation is 9.56%.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are included in the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the appended claims.
Claims (9)
1. The degradation method of the peach tree waste is characterized by comprising the following steps:
crushing peach branches into peach branch powder;
adding water, a carbon source and a composite degrading microbial inoculum into the peach branch powder for fermentation, wherein the fermentation environment temperature is 15-38 ℃, and the fermentation time is 80-90 days;
wherein the composite degrading bacteria agent comprises composite degrading bacteria liquid and an adsorbent, wherein the composite degrading bacteria liquid contains 3.60 multiplied by 10 strains9The content of the bacillus subtilis bacterial liquid/gram is more than 3.60 multiplied by 109The strain content of the yeast bacterial liquid is 2.40 multiplied by 10 per gram9The strain content of the rhodopseudomonas palustris bacterial liquid is more than 1.80 multiplied by 109Per gram or moreThe azotobacteria liquid and the content of the bacterial strain is 3.60 multiplied by 109More than one gram of trichoderma strain liquid, and the total number of bacteria in the composite degrading bacteria agent>4.5×109/g。
2. The degradation method according to claim 1,
the mass ratio of the peach branch powder, the water, the organic fertilizer and the composite degrading microbial inoculum is 4-5:3-5: 0.015: 0.005-0.01.
3. The degradation method according to claim 1,
the fermentation mode is natural ventilation composting fermentation.
4. The degradation process of claim 3, wherein the fermentation step comprises:
the method comprises the steps of recording the environmental temperature and the temperature of a stack body simultaneously from the loading day, measuring the temperature in the early morning every day, starting to turn the stack for the first time when the temperature of the stack body reaches above 60 ℃, measuring the temperature and the water of the stack body for the early, middle and late days every day, turning the stack once when the temperature of the stack body exceeds 60 ℃, and supplementing the water of the material to 60% when the water content of the stack body is lower than 50%.
5. The degradation method according to claim 1,
the size of the peach branch powder is 0.5-2.5cm, the carbon source is urea, and the adsorbent is turf or vermiculite.
6. The degradation method according to claim 1,
the bacillus subtilis liquid is obtained by activating bacillus subtilis and then inoculating the activated bacillus subtilis liquid into a bacillus subtilis culture medium for enrichment, wherein 1L of the bacillus subtilis culture medium contains 30g of corn flour, 35g of soybean meal and NaCL 5g and the balance 1L of distilled water;
wherein the enrichment condition is that air is purified, and oxygen is added to keep dissolved oxygen above 5 PPM; the fermentation temperature was 37 ℃.
7. The degradation method according to claim 1,
the yeast liquid is obtained by activating yeast, inoculating the activated yeast in a yeast culture medium, and enriching, wherein 1L of the culture medium comprises 40 g of starch, 15g of agar, and the balance of 1L of distilled water;
the enrichment condition is that the fermentation temperature is 25-28 deg.C, pH is 4-6, air is purified, and oxygen is added to maintain dissolved oxygen above 4 PPM.
8. The degradation method according to claim 1,
the trichoderma fungus liquid is obtained by activating trichoderma fungus and then inoculating the activated trichoderma fungus liquid into a fungus culture medium for enrichment, wherein 1L of the culture medium comprises 1L of 20% potato juice, 3g of monopotassium phosphate, 1.5g of magnesium sulfate and 20g of glucose;
the enrichment conditions are as follows: keeping the fermentation temperature at 28 deg.C, introducing purified air, and increasing oxygen to maintain dissolved oxygen above 5 PPM.
9. The degradation method according to claim 1,
the rhodopseudomonas palustris liquid is prepared by activating rhodopseudomonas palustris and then inoculating the activated rhodopseudomonas palustris liquid in a rhodopseudomonas palustris culture medium for enrichment; wherein 1L of rhodopseudomonas palustris culture medium comprises 30g of corn flour, 1g of monopotassium phosphate, 0.5g of magnesium sulfate and the balance of 1L of distilled water; the enrichment conditions are as follows: the fermentation temperature is 30 ℃, and the illumination intensity is 4000 and 5000 lux.
The azotobacteria liquid is prepared by activating azotobacteria and then inoculating the activated azotobacteria into an azotobacteria culture medium for enrichment; wherein 1L of azotobacter culture medium contains 10g of glucose, 1g of monopotassium phosphate, 0.2g of magnesium sulfate, 0.2g of calcium sulfate and the balance of 1L of distilled water; the enrichment conditions are as follows: introducing purified air, and maintaining the fermentation temperature at 25-28 deg.C.
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