CN114681652A - Reduction method and detection method of double-circle polished hydrogel contact lens - Google Patents
Reduction method and detection method of double-circle polished hydrogel contact lens Download PDFInfo
- Publication number
- CN114681652A CN114681652A CN202011628648.5A CN202011628648A CN114681652A CN 114681652 A CN114681652 A CN 114681652A CN 202011628648 A CN202011628648 A CN 202011628648A CN 114681652 A CN114681652 A CN 114681652A
- Authority
- CN
- China
- Prior art keywords
- protein
- contact lens
- double
- polished
- circumference
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000000017 hydrogel Substances 0.000 title claims abstract description 144
- 230000009467 reduction Effects 0.000 title claims abstract description 132
- 238000000034 method Methods 0.000 title claims abstract description 83
- 238000001514 detection method Methods 0.000 title claims abstract description 47
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 226
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 226
- 238000004140 cleaning Methods 0.000 claims abstract description 91
- QWPPOHNGKGFGJK-UHFFFAOYSA-N hypochlorous acid Chemical compound ClO QWPPOHNGKGFGJK-UHFFFAOYSA-N 0.000 claims abstract description 55
- 239000000523 sample Substances 0.000 claims abstract description 32
- 230000000694 effects Effects 0.000 claims abstract description 26
- 238000010828 elution Methods 0.000 claims abstract description 26
- 244000005700 microbiome Species 0.000 claims abstract description 15
- 238000006479 redox reaction Methods 0.000 claims abstract description 13
- 238000006722 reduction reaction Methods 0.000 claims description 128
- 238000005498 polishing Methods 0.000 claims description 40
- 239000004519 grease Substances 0.000 claims description 32
- 238000010494 dissociation reaction Methods 0.000 claims description 30
- 230000005593 dissociations Effects 0.000 claims description 29
- 238000001962 electrophoresis Methods 0.000 claims description 26
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 26
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 24
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 22
- 239000001301 oxygen Substances 0.000 claims description 22
- 229910052760 oxygen Inorganic materials 0.000 claims description 22
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 21
- 230000008569 process Effects 0.000 claims description 20
- 241000894006 Bacteria Species 0.000 claims description 16
- 125000004042 4-aminobutyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])N([H])[H] 0.000 claims description 12
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 claims description 12
- 230000000474 nursing effect Effects 0.000 claims description 12
- 241000700605 Viruses Species 0.000 claims description 11
- -1 hydrogen ions Chemical class 0.000 claims description 10
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 claims description 9
- DTQVDTLACAAQTR-UHFFFAOYSA-N Trifluoroacetic acid Chemical compound OC(=O)C(F)(F)F DTQVDTLACAAQTR-UHFFFAOYSA-N 0.000 claims description 9
- 210000004027 cell Anatomy 0.000 claims description 9
- 238000000354 decomposition reaction Methods 0.000 claims description 9
- 239000001257 hydrogen Substances 0.000 claims description 9
- 229910052739 hydrogen Inorganic materials 0.000 claims description 9
- 150000001413 amino acids Chemical class 0.000 claims description 8
- 238000000295 emission spectrum Methods 0.000 claims description 8
- 230000000813 microbial effect Effects 0.000 claims description 8
- ACZVWYLTJHGUCP-UHFFFAOYSA-N n-(2,2,2-trichloro-1-hydroxyethyl)formamide Chemical compound ClC(Cl)(Cl)C(O)NC=O ACZVWYLTJHGUCP-UHFFFAOYSA-N 0.000 claims description 8
- QDHHCQZDFGDHMP-UHFFFAOYSA-N Chloramine Chemical class ClN QDHHCQZDFGDHMP-UHFFFAOYSA-N 0.000 claims description 7
- 239000000460 chlorine Substances 0.000 claims description 7
- 230000001590 oxidative effect Effects 0.000 claims description 7
- 238000007254 oxidation reaction Methods 0.000 claims description 5
- 239000012466 permeate Substances 0.000 claims description 5
- 150000003384 small molecules Chemical class 0.000 claims description 5
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 claims description 4
- 102000004190 Enzymes Human genes 0.000 claims description 4
- 108090000790 Enzymes Proteins 0.000 claims description 4
- 125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 claims description 4
- 210000002421 cell wall Anatomy 0.000 claims description 4
- 229910052801 chlorine Inorganic materials 0.000 claims description 4
- 239000008367 deionised water Substances 0.000 claims description 4
- 229910021641 deionized water Inorganic materials 0.000 claims description 4
- 239000012634 fragment Substances 0.000 claims description 4
- 102000039446 nucleic acids Human genes 0.000 claims description 4
- 108020004707 nucleic acids Proteins 0.000 claims description 4
- 150000007523 nucleic acids Chemical class 0.000 claims description 4
- 238000007127 saponification reaction Methods 0.000 claims description 4
- 229910001415 sodium ion Inorganic materials 0.000 claims description 4
- 125000003277 amino group Chemical group 0.000 claims description 3
- 230000000593 degrading effect Effects 0.000 claims description 3
- 150000002431 hydrogen Chemical class 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 230000003301 hydrolyzing effect Effects 0.000 claims description 2
- 238000004070 electrodeposition Methods 0.000 abstract description 2
- 230000001012 protector Effects 0.000 abstract 1
- 235000018102 proteins Nutrition 0.000 description 186
- 239000000243 solution Substances 0.000 description 117
- 229910001868 water Inorganic materials 0.000 description 21
- 238000012360 testing method Methods 0.000 description 20
- 230000001954 sterilising effect Effects 0.000 description 17
- 238000002474 experimental method Methods 0.000 description 13
- 239000007788 liquid Substances 0.000 description 12
- 102000016943 Muramidase Human genes 0.000 description 11
- 108010014251 Muramidase Proteins 0.000 description 11
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 11
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 11
- 238000005516 engineering process Methods 0.000 description 11
- 229960000274 lysozyme Drugs 0.000 description 11
- 239000004325 lysozyme Substances 0.000 description 11
- 235000010335 lysozyme Nutrition 0.000 description 11
- 239000000463 material Substances 0.000 description 11
- 238000004659 sterilization and disinfection Methods 0.000 description 11
- PMZXXNPJQYDFJX-UHFFFAOYSA-N acetonitrile;2,2,2-trifluoroacetic acid Chemical compound CC#N.OC(=O)C(F)(F)F PMZXXNPJQYDFJX-UHFFFAOYSA-N 0.000 description 10
- 238000002791 soaking Methods 0.000 description 10
- 239000000607 artificial tear Substances 0.000 description 9
- 239000011521 glass Substances 0.000 description 9
- 150000002632 lipids Chemical class 0.000 description 8
- KZBUYRJDOAKODT-UHFFFAOYSA-N Chlorine Chemical compound ClCl KZBUYRJDOAKODT-UHFFFAOYSA-N 0.000 description 7
- 230000009471 action Effects 0.000 description 7
- 108010019783 tear proteins Proteins 0.000 description 7
- 235000001014 amino acid Nutrition 0.000 description 6
- 238000005868 electrolysis reaction Methods 0.000 description 6
- 239000011780 sodium chloride Substances 0.000 description 6
- 230000015556 catabolic process Effects 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 5
- 238000006731 degradation reaction Methods 0.000 description 5
- 239000008151 electrolyte solution Substances 0.000 description 5
- 238000000605 extraction Methods 0.000 description 5
- 230000031700 light absorption Effects 0.000 description 5
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 4
- 230000000844 anti-bacterial effect Effects 0.000 description 4
- 238000004364 calculation method Methods 0.000 description 4
- 238000005238 degreasing Methods 0.000 description 4
- 230000004438 eyesight Effects 0.000 description 4
- 238000000751 protein extraction Methods 0.000 description 4
- 239000012460 protein solution Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 206010061788 Corneal infection Diseases 0.000 description 3
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 3
- 238000005266 casting Methods 0.000 description 3
- 210000004087 cornea Anatomy 0.000 description 3
- 229940088598 enzyme Drugs 0.000 description 3
- 230000006870 function Effects 0.000 description 3
- 238000001179 sorption measurement Methods 0.000 description 3
- 229920002818 (Hydroxyethyl)methacrylate Polymers 0.000 description 2
- WOBHKFSMXKNTIM-UHFFFAOYSA-N Hydroxyethyl methacrylate Chemical compound CC(=C)C(=O)OCCO WOBHKFSMXKNTIM-UHFFFAOYSA-N 0.000 description 2
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 2
- 239000004472 Lysine Substances 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 108010052285 Membrane Proteins Proteins 0.000 description 2
- 208000003251 Pruritus Diseases 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000003750 conditioning effect Effects 0.000 description 2
- 239000002537 cosmetic Substances 0.000 description 2
- 230000015961 delipidation Effects 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 230000005684 electric field Effects 0.000 description 2
- 239000003792 electrolyte Substances 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- WIIZEEPFHXAUND-UHFFFAOYSA-N n-[[4-[2-(dimethylamino)ethoxy]phenyl]methyl]-3,4,5-trimethoxybenzamide;hydron;chloride Chemical compound Cl.COC1=C(OC)C(OC)=CC(C(=O)NCC=2C=CC(OCCN(C)C)=CC=2)=C1 WIIZEEPFHXAUND-UHFFFAOYSA-N 0.000 description 2
- 239000007800 oxidant agent Substances 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 239000002504 physiological saline solution Substances 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 239000001103 potassium chloride Substances 0.000 description 2
- 235000011164 potassium chloride Nutrition 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- VAZJLPXFVQHDFB-UHFFFAOYSA-N 1-(diaminomethylidene)-2-hexylguanidine Polymers CCCCCCN=C(N)N=C(N)N VAZJLPXFVQHDFB-UHFFFAOYSA-N 0.000 description 1
- DBCAQXHNJOFNGC-UHFFFAOYSA-N 4-bromo-1,1,1-trifluorobutane Chemical compound FC(F)(F)CCCBr DBCAQXHNJOFNGC-UHFFFAOYSA-N 0.000 description 1
- 206010002660 Anoxia Diseases 0.000 description 1
- 241000976983 Anoxia Species 0.000 description 1
- 239000004475 Arginine Substances 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 108090000565 Capsid Proteins Proteins 0.000 description 1
- 208000028006 Corneal injury Diseases 0.000 description 1
- 239000003109 Disodium ethylene diamine tetraacetate Substances 0.000 description 1
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 description 1
- 208000001860 Eye Infections Diseases 0.000 description 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- 206010021143 Hypoxia Diseases 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
- 102000018697 Membrane Proteins Human genes 0.000 description 1
- CERQOIWHTDAKMF-UHFFFAOYSA-N Methacrylic acid Chemical compound CC(=C)C(O)=O CERQOIWHTDAKMF-UHFFFAOYSA-N 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- RVGRUAULSDPKGF-UHFFFAOYSA-N Poloxamer Chemical compound C1CO1.CC1CO1 RVGRUAULSDPKGF-UHFFFAOYSA-N 0.000 description 1
- 229920002413 Polyhexanide Polymers 0.000 description 1
- 229920002385 Sodium hyaluronate Polymers 0.000 description 1
- 102400000368 Surface protein Human genes 0.000 description 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
- GLRAHDCHUZLKKC-UHFFFAOYSA-N acetonitrile;2,2,2-trifluoroacetic acid;hydrate Chemical compound O.CC#N.OC(=O)C(F)(F)F GLRAHDCHUZLKKC-UHFFFAOYSA-N 0.000 description 1
- 150000001370 alpha-amino acid derivatives Chemical class 0.000 description 1
- 235000008206 alpha-amino acids Nutrition 0.000 description 1
- 230000007953 anoxia Effects 0.000 description 1
- 239000004599 antimicrobial Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
- 239000004327 boric acid Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 235000018417 cysteine Nutrition 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- 235000019301 disodium ethylene diamine tetraacetate Nutrition 0.000 description 1
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
- 208000018459 dissociative disease Diseases 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- STVZJERGLQHEKB-UHFFFAOYSA-N ethylene glycol dimethacrylate Substances CC(=C)C(=O)OCCOC(=O)C(C)=C STVZJERGLQHEKB-UHFFFAOYSA-N 0.000 description 1
- 208000011323 eye infectious disease Diseases 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- 230000005802 health problem Effects 0.000 description 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
- 239000001866 hydroxypropyl methyl cellulose Substances 0.000 description 1
- 229920003088 hydroxypropyl methyl cellulose Polymers 0.000 description 1
- UFVKGYZPFZQRLF-UHFFFAOYSA-N hydroxypropyl methyl cellulose Chemical compound OC1C(O)C(OC)OC(CO)C1OC1C(O)C(O)C(OC2C(C(O)C(OC3C(C(O)C(O)C(CO)O3)O)C(CO)O2)O)C(CO)O1 UFVKGYZPFZQRLF-UHFFFAOYSA-N 0.000 description 1
- 235000010979 hydroxypropyl methyl cellulose Nutrition 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 239000003999 initiator Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 206010023332 keratitis Diseases 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 239000002103 nanocoating Substances 0.000 description 1
- 239000002777 nucleoside Substances 0.000 description 1
- 125000003835 nucleoside group Chemical group 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 201000005111 ocular hyperemia Diseases 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 239000008055 phosphate buffer solution Substances 0.000 description 1
- 125000001095 phosphatidyl group Chemical group 0.000 description 1
- 229960000502 poloxamer Drugs 0.000 description 1
- 229920001983 poloxamer Polymers 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 235000013772 propylene glycol Nutrition 0.000 description 1
- 238000002331 protein detection Methods 0.000 description 1
- 230000017854 proteolysis Effects 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 238000011946 reduction process Methods 0.000 description 1
- 239000012266 salt solution Substances 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 229940010747 sodium hyaluronate Drugs 0.000 description 1
- YWIVKILSMZOHHF-QJZPQSOGSA-N sodium;(2s,3s,4s,5r,6r)-6-[(2s,3r,4r,5s,6r)-3-acetamido-2-[(2s,3s,4r,5r,6r)-6-[(2r,3r,4r,5s,6r)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2- Chemical compound [Na+].CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 YWIVKILSMZOHHF-QJZPQSOGSA-N 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 230000009182 swimming Effects 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 229940124543 ultraviolet light absorber Drugs 0.000 description 1
- 239000006097 ultraviolet radiation absorber Substances 0.000 description 1
- 238000010200 validation analysis Methods 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 239000008215 water for injection Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L12/00—Methods or apparatus for disinfecting or sterilising contact lenses; Accessories therefor
- A61L12/02—Methods or apparatus for disinfecting or sterilising contact lenses; Accessories therefor using physical phenomena, e.g. electricity, ultrasonics or ultrafiltration
- A61L12/023—Electrolysis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L12/00—Methods or apparatus for disinfecting or sterilising contact lenses; Accessories therefor
- A61L12/08—Methods or apparatus for disinfecting or sterilising contact lenses; Accessories therefor using chemical substances
- A61L12/086—Container, accessories or devices therefor
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L12/00—Methods or apparatus for disinfecting or sterilising contact lenses; Accessories therefor
- A61L12/08—Methods or apparatus for disinfecting or sterilising contact lenses; Accessories therefor using chemical substances
- A61L12/10—Halogens or compounds thereof
- A61L12/107—Hypohalites; Active halogens
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4044—Concentrating samples by chemical techniques; Digestion; Chemical decomposition
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/33—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using ultraviolet light
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N21/643—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" non-biological material
-
- G—PHYSICS
- G02—OPTICS
- G02C—SPECTACLES; SUNGLASSES OR GOGGLES INSOFAR AS THEY HAVE THE SAME FEATURES AS SPECTACLES; CONTACT LENSES
- G02C13/00—Assembling; Repairing; Cleaning
- G02C13/008—Devices specially adapted for cleaning contact lenses
Landscapes
- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- General Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Pathology (AREA)
- Analytical Chemistry (AREA)
- Public Health (AREA)
- Epidemiology (AREA)
- General Chemical & Material Sciences (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Optics & Photonics (AREA)
- Molecular Biology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Ophthalmology & Optometry (AREA)
- Eyeglasses (AREA)
Abstract
The invention discloses a reduction method and a detection method of a double-circumference polished hydrogel contact lens, wherein a reduction instrument is started, two probes oppositely arranged in a reduction tank are converted into a positive electrode and a negative electrode, protein attached to the surface of the double-circumference polished hydrogel contact lens carries charges in a care solution, the charged protein moves towards an electrode position with the opposite electrical property of the charged protein, and a protector is arranged on the surface of the double-circumference polished hydrogel contact lensCl in the treating solution‑Generating hypochlorous acid, wherein the hypochlorous acid and the protein have redox reaction in a reduction tank, the protein is degraded, the hypochlorous acid inactivates the functional protein of the microorganism, and the microorganism is killed; the detection method is characterized in that the protein elution rate of the reduction instrument matched with the care solution to the double-week polished hydrogel contact lens is calculated by detecting the protein content before and after the lens is cleaned. The reduction method and the detection method effectively solve the problems that the corneal contact lens is difficult to reduce and the cleaning effect is difficult to evaluate.
Description
Technical Field
The invention relates to the field of corneal contact lenses, in particular to a reduction method and a detection method of a double-circumference-polishing hydrogel contact lens.
Background
The problem of how to remove protein from contact lenses troubles the industry for more than half a century, and causes high importance of contact lens wearing safety in the eye vision industry of various countries. Because the cases of corneal infection on the corneal contact lens are frequent in China, the contact lens is listed as a third class of medical instruments in 2012 and is used as high-risk management, and the reasons are as follows: the contact lens material structure has a large number of fiber itching-penetrating holes invisible to naked eyes, a large number of tears are secreted by human eyes at any time, a large number of lacrimal proteins are contained in the tears, and the lacrimal proteins easily permeate into the fiber itching-penetrating holes to cause the DK value of the lens to be reduced (oxygen permeability), the symptoms of corneal anoxia, edema and the like to be caused, and the problems of corneal damage, bacterial infection, corneal inflammation, even vision damage and the like to be caused seriously.
In order to be matched with a corneal contact lens for cleaning and restoring to ensure the eye safety of a user, a plurality of cleaners for cleaning and restoring the worn corneal contact lens are also provided in the market, the cleaning and restoring effects of the cleaners on corneal contact lenses of different specifications and models are not verified, so that potential safety hazards are buried, if the cleaners are limited in cleaning and restoring the corneal contact lens by matching with cleaning liquid, the user still cleans and restores the corneal contact lens by using the cleaners under the condition that the cleaners are not applicable, and thus the eye safety problem of the user can be exploded after the cleaner is used for a while, so that the eye safety of the user is not responsible for the eye safety of the user. Contact lenses of various specifications are currently marketed,double-circle polishingThe hydrogel contact lens has a service cycle of half a month, so that compared with daily lens polishing, the cost can be saved when a user uses the contact lens for two weeks, and compared with monthly lens polishing, the service cycle of the lens for two weeks is short, and the safety is higher. Therefore, the temperature of the molten metal is controlled,double-circle polishingHydrogel contact lenses are also a more common product for ophthalmic use. Therefore, it is a health problem concerned by users to effectively remove proteins on the surface of the double-week-polished hydrogel contact lens and ensure the safety of eyes, and therefore, a protein removing and sterilizing method aiming at the double-week-polished hydrogel contact lens is necessary to provide the safety of eyes of usersAnd (5) protecting driving and navigating.
The market has also now introduced various methods for removing tear protein from the surface of contact lenses, but since it is not visible to the naked eye whether the tear protein is completely efficiently eluted and degraded, in order to test the cleaning effect of the cornea contact lens cleaned and restored by the cleaner and ensure the eye safety of consumers, a gradual protein detection method, a sterilization detection method and the like are focused and developed, so as to effectively verify whether the protein, bacteria and the like are completely degraded and killed by scientific detection, however, the detection method for effectively removing the protein of the corneal contact lens in the market at the present stage has limitations, and a specific quantity value which can directly, effectively and accurately measure the tear protein adsorbed by the corneal contact lens cannot be found, therefore, the detection result is not comprehensive, has a certain reference significance, and cannot be a professional detection basis for quantitatively and qualitatively detecting the protein degradation degree. Therefore, it is necessary to provide a method forDouble-circle polishingThe method for detecting the protein-removing and sterilizing effects of the hydrogel contact lens has the advantages that the detection method is used for detecting the effects of the protein-removing and sterilizing method aiming at the double-week-polishing of the hydrogel contact lens, the obtained detection result is a guarantee for a user, and the protein-removing and sterilizing method can guide the user to correctly perform protein-removing, sterilizing and cleaning on the double-week-polishing of the hydrogel contact lens, so that the eye infection rate of the user is reduced, and the eye safety of the user is guaranteed.
Therefore, a new technical solution is needed to solve the above problems.
Disclosure of Invention
One purpose of the invention is to provide a reduction method for a double-circumference-polished hydrogel contact lens, which can remove adsorbed proteins, bacteria and lipid on the surface and in an oxygen permeable hole of the double-circumference-polished hydrogel contact lens, realize the reduction of the double-circumference-polished hydrogel contact lens, provide an effective cleaning mode for the double-circumference-polished hydrogel contact lens for vast consumers, and ensure the safety of lens entering eyes; the invention also aims to provide a reduction detection method of the double-circumference polished hydrogel contact lens, which can effectively verify the reduction effect of the reduction method on the double-circumference polished hydrogel contact lens, the detection method is used for detecting bacteria, proteins and lipids which are invisible to naked eyes, and a detection result is provided for a consumer through a chemical experiment method, so that the reduction method can clean and reduce the double-circumference polished hydrogel contact lens, the lenses cleaned by the cleaner are safe and harmless from a scientific angle, and the eye safety of the user is further guaranteed.
On one hand, the invention provides a reduction method aiming at a double-circumference polished hydrogel contact lens, which adopts the following technical scheme:
a method of reducing a bi-weekly-polished hydrogel contact lens, comprising: adding a care solution and a double-circumference-polishing hydrogel contact lens into a cleaning tank of a reduction instrument, wherein two electrophoresis dissociation probes are oppositely arranged in the cleaning tank, starting the reduction instrument, the two electrophoresis dissociation probes are electrified, one electrophoresis dissociation probe is a positive electrode, the other electrophoresis dissociation probe is a negative electrode, the protein attached to the surface of the double-circumference-polishing hydrogel contact lens is charged in the care solution, the charged protein moves towards the electrode position opposite to the electric property of the charged protein, and Cl in the care solution-Moving towards the positive electrode, losing electrons and oxidizing the electrons into chlorine, dissolving the chlorine into the care solution to generate hypochlorous acid, and performing redox reaction on the hypochlorous acid and protein in the cleaning tank to degrade the protein; the hypochlorous acid generates self-oxidation-reduction reaction in the care solution to decompose hydrogen ions, chloride ions and oxygen, and absorbs electrons of functional proteins of microorganisms in the cleaning tank in the decomposition process so as to inactivate the functional proteins of the microorganisms, so that the microorganisms are killed.
Further in the above technical solution, it further includes: grease attached to the surface of the double-circumference polished hydrogel contact lens is removed, wherein hydrogen ions with positive charges in the care solution move to the negative electrode and undergo a reduction reaction near the negative electrode to generate hydrogen, sodium ions and hydroxyl ions in the care solution generate sodium hydroxide, the grease on the surface of the double-circumference polished hydrogel contact lens and the sodium hydroxide undergo a saponification reaction, and the grease on the surface of the double-circumference polished hydrogel contact lens is removed.
Further, hypochlorous acid and protein undergo redox reaction in the cleaning tank, and the protein is degraded, which specifically comprises:
reacting hypochlorous acid with a peptide chain forming a protein skeleton to obtain chloral formamide, hydrolyzing the peptide chain formed by the chloral formamide in a solution, breaking peptide bonds in the peptide chain, decomposing the peptide chain into micromolecule protein and amino acid, degrading the protein, and/or,
the hypochlorous acid reacts with the side chains of the peptide chains forming the protein skeleton, the side chains comprise lysine side chains, the hypochlorous acid reacts with the lysine side chains to form chloramines on the lysine side chains of the protein and the amino groups of the protein, the chloramines are decomposed to form organic molecule fragments [ 1,2 ] in a carbonyl form, peptide bonds on the lysine side chains are broken and decomposed into small-molecule proteins and amino acids, and the proteins are degraded.
Further, the microorganisms include bacteria and viruses, the hypochlorous acid absorbs electrons of functional proteins on cell walls of the bacteria during decomposition, and the bacteria are inactivated; the hypochlorous acid absorbs electrons of functional proteins on the outer shell of the virus in a decomposition process, and the virus is inactivated; the hypochlorous acid has strong oxidizing property, permeates into the microbial cells, and is subjected to oxidation reaction with mycoprotein, nucleic acid and enzyme in the microbial cells so as to kill the microbes.
In another aspect, the present invention further provides a reduction detection method for a double-circumference polished hydrogel contact lens, which comprises: the method for detecting the elution rate of the protein on the double-week-polishing hydrogel contact lens reduced by the reduction method specifically comprises the following steps:
detecting the protein content adsorbed on the surface of the double-circumference-polishing hydrogel soft hydrophilic contact lens to obtain the original protein content of the lens, placing the double-circumference-polishing hydrogel soft hydrophilic contact lens in a reduction instrument to be matched with a care solution for cleaning, detecting the protein content of the double-circumference-polishing hydrogel soft hydrophilic contact lens cleaned by the reduction instrument to obtain the residual protein content of the cleaned lens, and performing calculation analysis on the residual protein content of the cleaned lens and the original protein content of the lens to obtain the elution rate of the reduction instrument matched with the care solution on the protein adsorbed on the surface of the double-circumference-polishing hydrogel soft hydrophilic contact lens and in the oxygen permeation holes.
The technical scheme is further that the double-circumference-polishing hydrogel soft hydrophilic contact lens is placed in a reduction instrument and is matched with a care solution for cleaning, and the method specifically comprises the following steps:
the double-circumference polished hydrogel soft hydrophilic contact lens is placed in a cleaning tank of a reduction instrument and is matched with a nursing solution for cleaning for 3 minutes, the voltage difference value between two electrophoresis dissociation probes in the cleaning tank is 0.4V-4.9V in the cleaning process, and the current value is 0.4-4 mA.
Further, detecting the protein content of the double-circumference polished hydrogel soft hydrophilic contact lens treated by the reduction instrument to obtain the residual protein content of the cleaned lens, which specifically comprises the following steps:
and placing the double-circumference polished hydrogel soft hydrophilic contact lens treated by the reduction instrument into a protein extracting solution, vibrating to extract protein, detecting a protein concentration value in the protein extracting solution by using a micro ultraviolet spectrophotometer, and calculating according to the protein concentration value to obtain the residual protein amount of the cleaned lens.
Further, the protein extracting solution is formed by mixing 50 parts of acetonitrile, 50 parts of deionized water and 0.2 part of 100% trifluoroacetic acid; calculating and analyzing the residual protein amount of the cleaned lens and the original protein amount of the lens to obtain the elution rate of the reduction instrument matched with the care solution on the protein adsorbed on the surface and in the oxygen permeation holes of the double-circumference polished hydrogel soft hydrophilic contact lens:
the above technical solution further includes: the detection of the surface grease removing effect of the double-circumference polished hydrogel contact lens reduced by the reduction method specifically comprises the following steps:
detecting the oil content on the surface of the double-circumference polished hydrogel contact lens with the surface adsorbed with oil,
and adding a care solution into a cleaning tank of the reduction instrument, placing the double-circumference polished hydrogel contact lens with the surface adsorbed with the grease into the care solution, starting the reduction instrument, detecting the grease content on the surface of the double-circumference polished hydrogel contact lens cleaned by the reduction instrument after the cleaning is finished, and measuring the grease removal rate of the reduction instrument matched with the care solution to the double-circumference polished hydrogel contact lens.
Further, emitting a light beam to the double-circumference polished hydrogel contact lens with the surface adsorbed with the grease through a fluorescence spectrophotometer, and recording a fluorescence intensity value of an emission spectrum, wherein the fluorescence intensity value corresponds to the grease content on the surface of the double-circumference polished hydrogel contact lens;
compared with the prior art, the invention has one or more of the following beneficial effects:
1. the invention provides a reduction method of a double-circumference-polishing hydrogel contact lens, which realizes the sterilization of protein removal by electrophoretic dissociation through a reduction instrument matched with a care solution, two electrophoretic dissociation probes are oppositely arranged in a cleaning tank of the reduction instrument, the two electrophoretic dissociation probes can be converted into a positive electrode and a negative electrode under the power-on state of the reduction instrument to form the basis of protein electrophoresis, the two electrodes can generate hypochlorous acid with strong oxidability under the action of chloride ions in the care solution, the hypochlorous acid can degrade proteins and can sterilize, and sodium hydroxide generated by sodium ions in sodium chloride and hydroxyl ions in the main component of the care solution can remove grease, so the reduction method can effectively clean and reduce the double-circumference-polishing hydrogel contact lens.
2. The invention provides a reduction method of a double-circumference-polishing hydrogel contact lens, which combines a protein electrophoresis principle with an electrolysis technology to obtain a new electrophoresis dissociation reduction technology, the technology is matched with a care solution (containing chloride ions), hypochlorous acid can be generated in a cleaning tank, the hypochlorous acid can be used as a strong oxidizer to absorb electrons of surface protein of cell walls of bacteria, so that functional protein on the surfaces of the bacteria is oxidized, the bacteria can not absorb nutrition, can not metabolize normally and stop splitting to be inactivated, and the sterilization and disinfection effects are finally achieved; hypochlorous acid, as a strong oxidant, can also absorb electrons from surface proteins on the viral coat, causing viral inactivation; hypochlorous acid can not only act on the surfaces of microorganisms, but also permeate into microbial cells to perform oxidation reaction with organic macromolecules such as thallus (virus) protein, nucleic acid, enzyme and the like in the microbial cells so as to kill pathogenic microorganisms, so that the reduction method has double effects of protein removal and sterilization.
3. The characteristic that hypochlorous acid can degrade tear protein is utilized by the reduction method, so that hypochlorous acid and the sedimentary protein are subjected to oxidation reaction to decompose the stubborn sedimentary protein into micromolecular protein and amino acid, and the micromolecular protein is easier to adsorb than the sedimentary protein, so that the sedimentary protein on the double-circumference-polished hydrogel contact lens can be effectively removed by the reduction method.
4. The protein is degraded by hypochlorous acid, and the hypochlorous acid degradation of the protein comprises the following two modes, one mode is that the skeleton peptide chain of the protein is directly degraded, the degradation mode is that hypochlorous acid reacts with the peptide skeleton to form chloral formamide, the skeleton in the form of the chloral formamide is easy to hydrolyze in aqueous solution, so that peptide bonds are broken, and the protein is degraded; the second is to degrade the side chain on the peptide skeleton by hypochlorous acid, arrange according to the reactivity of the side chain and hypochlorous acid, the side chain is methionine > cysteine > histidine > alpha-amino acid > tryptophan > lysine > tyrosine > arginine > glutamic acid, unstable chloramine is formed on the side chains of amino and lysine, and is finally decomposed into organic molecular fragments (1, 2) in a carbonyl form, the two reaction degradation modes are that the tear protein is decomposed into small molecular protein to help electrophoretic adsorption, the protein removing effect is more thorough, certainly, due to the diversity of protein molecules, the degradation mode of the protein by hypochlorous acid is more than two, the invention only provides two effective degradation modes, therefore, the protein removing method provided by the invention can effectively remove the protein adsorbed on the surface of the double-circumference polished contact lens and in the oxygen permeable holes.
5. The restoring method of the double-circumference polishing hydrogel contact lens provided by the invention generates a restoring effect through the combined action of the restoring instrument and the care solution, deeply discloses the principle of cleaning and restoring the lens through an electrophoresis dissociation technology, and explains the effectiveness of the method from the biochemical point of view.
6. The invention also provides a reduction detection method of the double-circumference polishing hydrogel contact lens, which calculates and obtains the protein elution rate of the double-circumference polishing hydrogel contact lens by using the reduction method through detecting the content of protein adsorbed on the surface and in the oxygen permeable pores before and after the cleaning of the double-circumference polishing lens, and the content of the protein adsorbed on the surface and in the oxygen permeable pores before and after the cleaning of the lens is detected through vibration extraction of the protein extracting solution, in the prior art, the specific content of the lachrymal protein adsorbed by the corneal contact lens is difficult to measure, the invention realizes the extraction of the protein through the protein extracting solution consisting of acetonitrile-pure water-trifluoroacetic acid mixed solution, and then detects the protein content in the protein extracting solution by matching with an ultraviolet spectrophotometer to measure the specific content of the lachrymal protein adsorbed by the corneal contact lens, and the protein separating solution has no reaction with the corneal contact lens material, and (3) taking the cornea contact lens cultured by artificial tears as a detection sample without influencing the measured value of the protein, placing the detection sample in the protein separating liquid to carry out vibration extraction on the protein, and calculating to obtain the elution rate of the protein separating liquid on the protein.
7. The invention also provides a reduction detection method of the double-circumference polished hydrogel contact lens, the detection method utilizes a fluorescence spectrophotometer to emit light beams to the double-circumference polished hydrogel contact lens with the surface adsorbed with the grease, records the fluorescence intensity value of an emission spectrum so as to obtain the grease adhesion condition on the lens before and after cleaning, and then obtains the cleaning effect of the reduction instrument matched with the nursing solution on the surface of the double-circumference polished hydrogel contact lens through calculation and analysis.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments of the present invention, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.
In the description of the present invention, it is to be understood that the terms are to be interpreted broadly, unless otherwise explicitly defined and limited, and the specific meanings of the terms in the present invention may be understood by those skilled in the art as appropriate.
The gist of the present invention is further illustrated by the following examples.
Example 1:
in 2012, contact lenses are classified into a third class of medical devices as high risk management, and care solution products used in combination with contact lenses for cleaning are also classified into the third class of medical devices. The care solution for cleaning in conjunction with a contact lens contains a variety of chemical components including, but not limited to, sodium chloride, preservatives, surfactants, and antimicrobial agents. The third medical instrument nursing liquid can cause eye redness under the condition of improper purchase and use, corneal infection cases caused by corneal contact lenses are frequent, and the double-week-polishing hydrogel contact lenses with good market quantity also have the risk of corneal infection caused by incomplete cleaning, so the cleaning and restoring problems of the double-week-polishing hydrogel corneal contact lenses are also the focus of public attention. The invention is focused on providing a lens cleaning and restoring method for the double-circumference polished hydrogel contact lens, and the double-circumference polished hydrogel contact lens is cleaned and restored by matching an electrophoresis dissociation technology with a care solution, so that the double-circumference polished hydrogel contact lens after being worn and used can be effectively cleaned, and the eye safety is ensured.
The invention provides a reduction method of a double-circumference polished hydrogel contact lens, which comprises the following steps: providing a reduction instrument, wherein the reduction instrument is provided with a cleaning tank, at least two electrophoresis dissociation probes are oppositely arranged in the cleaning tank, a care solution is added into the cleaning tank, a double-week-polishing hydrogel contact lens is placed in the care solution, the reduction instrument is started, and the two-week-polishing hydrogel contact lens is used for polishing the electrophoresis dissociation probes,
the two electrophoresis dissociation probes form a positive electrode and a negative electrode after being electrified, the protein attached to the surface of the double-circumference polished hydrogel contact lens carries charges in the care solution, the charged protein moves towards the position of the electrode with the opposite electric property (namely protein electrophoresis phenomenon), and Cl in the care solution-Moving toward the positive electrode and losing electrons to be oxidized into chlorine gas, the chlorine gas is dissolved in the care solution to generate hypochlorous acid (namely, an electrolysis technology), the hypochlorous acid and the protein undergo redox reaction in the cleaning tank, and the protein is degraded. This is the principle of action of protein removal by electrophoretic dissociation technology. The electrophoresis dissociation probe is a nano-scale coating material electrophoresis dissociation probe, and the probe can promote electrophoresis and dissociation reaction in a protein removal sterilization tank under the combined action of a protein removal sterilization instrument and physiological saline (or electrolyte solution).
Further illustrating the principles of electrophoretic dissociation protein removal according to the present invention:
the method for cleaning and reducing the lens through electrophoretic dissociation mainly superposes a protein electrophoresis technology and an electrolysis technology. Firstly, the protein Electrophoresis technology (Electrophoresis) refers to the phenomenon that charged particles or molecules move in an electric field, large molecular proteins, polypeptides, virus particles, even amino acids of cells or small molecules, nucleosides and the like can perform directional swimming in the electric field, and under the action of the contact lens cleaner, the tear protein is charged and moves towards the position of a probe electrode under the action of the probe field intensity in a cleaning tank of a cleaning chamber lens, so that the effect of cleaning a corneal contact lens is achieved. Further, the electrolysis technique refers to a process of causing an oxidation-reduction reaction at a cathode and an anode after passing an electric current through an electrolyte solution or a molten electrolyte (also referred to as an electrolyte).
In one embodiment, the invention uses the care solution as an electrolyte solution for cleaning the lens, chloride ions in the care solution move to the positive electrode probe in the electrolysis process and lose electrons to be reduced into chlorine gas, the care solution with a certain concentration generates the chlorine gas, a part of the chlorine gas is discharged by bubbles, a part of the chlorine gas is dissolved in water, and the following chemical reactions occur:
Cl2+H2O＝HCl+HClO
the HClO generated by the reaction has the functions of degrading protein and sterilizing.
The above theory demonstrates that the electrolyte solution used in the method of the present invention for cleaning and reducing the lens by electrophoretic dissociation can be any heavy metal-free solution containing chloride ions, and even ordinary care solutions (basically all commercial care solutions contain chloride ions) can be used as the electrolyte solution of the present invention, such as NaCl solution, KCl solution, MgCl2 solution, and Cl-containing eye care solutions, which are more conventional, or a combination of a plurality of these solutions (provided that no reaction occurs and electrolysis is destroyed so that no hypochlorous acid can be generated in the lens cleaning tank). Thus, any solution containing chloride ions that may be used in the reduction process of the present invention may serve the dual purpose of protein removal and sterilization.
In one embodiment, the hypochlorous acid and the protein are subjected to redox reaction in a cleaning tank of a reduction apparatus, the protein is degraded, chloral formamide is obtained by reacting the hypochlorous acid with a peptide chain forming a protein skeleton, the peptide chain formed by the chloral formamide is hydrolyzed in a solution, peptide bonds in the peptide chain are broken, and the peptide chain is decomposed into small-molecule protein and amino acid, so that the protein is degraded.
In another embodiment, the hypochlorous acid reacts with the protein in the washing tank to perform redox reaction, so that the protein is degraded, and the hypochlorous acid reacts with the side chain of the peptide chain forming the protein skeleton, wherein the side chain comprises a lysine side chain, the hypochlorous acid reacts with the lysine side chain to form chloramine on the lysine side chain of the protein and the amino group of the protein, the chloramine is decomposed to form organic molecule fragments [ 1,2 ] in a carbonyl form, and the peptide bond on the lysine side chain is broken to be decomposed into small-molecule protein and amino acid, so that the protein is degraded.
In another embodiment, the hypochlorous acid reacts with the protein in the cleaning tank in a redox reaction, and the protein is degraded by reacting the hypochlorous acid with a peptide chain forming a protein skeleton and a side chain of the peptide chain forming the protein skeleton, so that the protein is degraded.
The hypochlorous acid generates self-oxidation-reduction reaction in the care solution to decompose hydrogen ions, chloride ions and oxygen, and absorbs electrons of functional proteins of microorganisms in the cleaning tank in the decomposition process so as to inactivate the functional proteins of the microorganisms, and the microorganisms are killed. This is the principle of action of sterilizing by using the strong oxidizing property of hypochlorous acid. In one embodiment, the microorganisms adhered to the corneal contact lens include bacteria and viruses, the hypochlorous acid absorbs electrons of functional proteins on cell walls of the bacteria during decomposition, and the bacteria are inactivated; the hypochlorous acid absorbs electrons of functional proteins on the outer shell of the virus in a decomposition process, and the virus is inactivated; the hypochlorous acid has strong oxidizing property, permeates into the microbial cells, and is subjected to oxidation reaction with mycoprotein, nucleic acid and enzyme in the microbial cells so as to kill the microbes. All of these sterilization methods utilize the strong oxidizing property of hypochlorous acid.
Certainly, the nursing liquid sold in the market generally contains sodium chloride and a bactericide, so the nursing liquid has a certain bactericidal effect (the contact lenses are generally soaked in the nursing liquid for 5 to 6 hours to achieve the bactericidal effect), but the reduction instrument provided by the invention is matched with the nursing liquid to remove protein and sterilize, so that the sterilization effect can be further enhanced, the sterilization promotion effect is invalid, and the nursing liquid does not need to wait for 5 to 6 hours to achieve the bactericidal effect like the existing soaking and cleaning mode.
In the technical scheme, the reduction instrument is started, the hydrogen ions with positive charges in the care solution move to the negative electrode and undergo a reduction reaction near the negative electrode to generate hydrogen, the sodium ions and the hydroxide ions in the care solution generate sodium hydroxide, the grease on the surface of the double-circumference polished hydrogel contact lens and the sodium hydroxide undergo a saponification reaction, and the grease on the surface of the double-circumference polished hydrogel contact lens is hydrolyzed. This is the delipidation effect brought by the reduction method of the invention, the change of the pH value of the solution is promoted during the reduction, and when the lens is attached with lipid, the electrophoretic dissociation can promote the saponification reaction, so that when the lens is attached with lipid, the electrophoretic dissociation reduction method of the invention can carry out delipidation smoothly.
The conventional soft hydrophilic contact lens made of double-circumference polishing type hydrogel has very low cleaning efficiency and less than 10% of protein removal effect if daily cleaning is carried out in a mode of rubbing the lens by hands and soaking the lens in a care solution, and the soft hydrophilic contact lens is mainly composed of hydroxyethyl methacrylate which is a colloid material, so that the scratch and damage of the lens are easily caused by rubbing with fingers, and the service life of the lens is shortened. The reduction method of the invention safely and efficiently removes protein and sterilizes through an electrophoretic dissociation technology, can remove lipid along the way when grease is attached to the surface of the lens, achieves multiple purposes, is efficient to clean and can quickly reduce.
Example 2:
in order to detect the cleaning effect of the reduction method on the double-circumference polished hydrogel contact lens, the invention also provides a reduction detection method of the double-circumference polished hydrogel contact lens, which comprises the following steps: the method for detecting the elution rate of the reduction instrument matched with the care solution on the protein adsorbed on the surface of the double-circumference-polishing hydrogel contact lens and in the oxygen permeation hole specifically comprises the following steps:
detecting the protein content adsorbed on the surface of the double-circumference polished hydrogel soft hydrophilic contact lens to obtain the original protein content of the lens;
placing the double-circumference polished hydrogel soft hydrophilic contact lens in a reduction instrument to perform lens cleaning reduction treatment by matching with a care solution: placing the double-circumference polished hydrogel soft hydrophilic contact lens in a cleaning tank of a reduction instrument to be cleaned for 3 minutes by matching with a care solution, wherein the voltage difference value between two electrophoresis dissociation probes in the cleaning tank is 0.4-4.9V and the current value is 0.4-4mA in the cleaning process;
detecting the protein content of the double-circumference polished hydrogel soft hydrophilic contact lens treated by the reduction instrument to obtain the residual protein content of the cleaned lens: placing the double-circumference polished hydrogel soft hydrophilic contact lens treated by the reduction instrument in a protein extracting solution, vibrating to extract protein, detecting a protein concentration value in the protein extracting solution by a micro ultraviolet spectrophotometer, and calculating according to the protein concentration value to obtain the residual protein amount of the cleaned lens;
calculating and analyzing the residual protein amount of the cleaned lens and the original protein amount of the lens to obtain the elution rate of the reduction instrument matched with the care solution on the protein adsorbed on the surface and in the oxygen permeable holes of the double-circumference polished hydrogel soft hydrophilic contact lens:
in one embodiment, the protein extract is composed of a mixture of 50 parts acetonitrile, 50 parts deionized water, and 0.2 parts 100% trifluoroacetic acid. The main principle attached to the detection method is that tear protein adsorbed on the surface and in the inner oxygen permeable holes of the corneal contact lens can be extracted by 100% according to a mixed solution of acetonitrile-trifluoroacetic acid-water, the protein extraction solution does not react with corneal contact lens materials and does not influence the measured value of protein, so that the corneal contact lens used as a detection sample can be placed in the protein extraction solution to extract and separate protein in a vibration mode, the concentration of the protein in the protein extraction solution is detected through a micro ultraviolet spectrophotometer, and the content value of the adsorbed protein of the lens can be obtained through simple calculation of the concentration of the protein.
It should be noted that the known protein-extracting substance at this stage is pbs solution (phosphate buffer solution, a standard salt solution), but it also has a drawback that it cannot sufficiently extract proteins from the corneal contact lens. Therefore, no accepted method is available for testing the actual protein content adsorbed on the contact lens worn by human eyes, but in order to prove the effectiveness of the method, the experiment is carried out by the contact lens cultured by artificial tears, and on the premise that no method can specifically measure the actual protein content adsorbed on the contact lens, the experiment defaults that the actual protein content adsorbed on the contact lens cultured from the artificial tears is the theoretical content of the lens adsorbed protein obtained by calculation.
The experimental procedure for the validation discussed above is as follows:
1. mixing 50 parts of acetonitrile, 50 parts of deionized water and 0.2 part of 100% trifluoroacetic acid to obtain the protein extracting solution;
2. taking an FDA classified class IV soft hydrophilic contact lens, culturing in a centrifuge tube containing 1ml of artificial tears (2.2mg/ml) at constant temperature of 37 ℃ for 1 day, taking out the lens, actually measuring the protein concentration in the centrifuge tube to be 1.328mg/ml, and the theoretical value of the protein content absorbed by the lens to be 2.2-1.328-0.872 mg,
3. the lens cultured with the artificial tear in the step 2 was sufficiently dissolved in 1ml or 4ml of the protein extract, and the lens was vibrated at room temperature for 24 hours, and the protein concentration was actually measured to be 0.805mg/ml after the vibration extraction, and therefore, the protein extraction rate of the protein extracted with the protein extract was calculated to be 0.805/0.872 ═ 100% ═ 92.3%.
The protein extracting capability of the protein extracting solution is verified through the experiment, so that the protein extracting solution can be used for carrying out an experiment of extracting and detecting the protein concentration.
Example 3:
an experiment is carried out according to the detection method in the embodiment 2, so as to detect the elution rate of the reduction instrument matched with the care solution on the protein adsorbed on the surface and in the oxygen permeation holes of the double-circumference polished hydrogel contact lens in the embodiment, and the experimental steps are as follows:
1. eye wear Qiangsheng Anyanyou (an eye with good vision)
2Brand Contact Lenses) double-circumference disposable hydrogel soft hydrophilic Contact lens (national mechanical injection 20193162150, LOT No.: L004D9J) for 8 hours, lens material: etafilcon a hydrogel, water content: 59 percent of water;
2. after the human eyes are worn for 8 hours, taking the left and right glasses lenses and respectively putting the left and right glasses lenses into a 1.5ml centrifugal tube, adding 1ml acetonitrile-trifluoroacetic acid solution into the centrifugal tube, testing the lysozyme amount in the centrifugal tube after vibrating for 30 minutes, wherein the lysozyme amount is 0.406mg and 0.410mg respectively, and measuring that the average adsorbed protein amount of the glasses lens worn for 1 day (8 hours) is 0.408mg, namely considering that the original protein amount of the glasses lens is 0.408 mg;
3. repeating the step 1, and the same person wears the eyes which are good for life and eyesight (
2Brand Contact Lenses) double-circumference polishing type hydrogel soft hydrophilic Contact Lenses (national mechanical entry 20193162150, LOT No.: L004D9J) for 8 hours to obtain two pieces of corneal Contact Lenses with protein on the surfaces;
4. taking a piece of corneal contact lens in the step 3, placing the corneal contact lens in 1ml of Haichang water feeling care solution (national mechanical Standard 20153160333) for soaking for 3 minutes, then placing the corneal contact lens in a centrifuge tube of 1.5ml, adding 1ml of acetonitrile-trifluoroacetic acid solution into the centrifuge tube, vibrating for 30 minutes, and testing the lysozyme amount in the centrifuge tube to be 0.396 mg;
5. placing the other corneal contact lens in the step 3 in a cleaning tank of a reduction instrument, adding 1ml of Haichang water feeling care solution (national mechanical Standard 20153160333), starting the reduction instrument for cleaning for 3 minutes, wherein the voltage at two ends of a measuring probe in the cleaning process is 4.5V, and the current is 3.57mA (the voltage at two ends of the measuring probe in the cleaning process can be 0.4V-4.9V, and the current is 0.4-4mA), placing the corneal contact lens into a 1.5ml centrifuge tube after the cleaning process is finished, adding 1ml of acetonitrile-trifluoroacetic acid solution into the centrifuge tube, and testing the lysozyme amount in the centrifuge tube to be 0.035mg after vibrating for 30 minutes;
6. calculating the elution rate of the reduction instrument matched with the care solution on the protein adsorbed on the surface of the double-circumference polished hydrogel contact lens and in the oxygen permeation hole:
similarly, the protein elution rate for soaking the two-week-cast hydrogel contact lens with the conditioning solution was calculated to be equal to (1-0.396mg/0.408mg) × 100% — 2.9%.
The Haichang water feeling care solution (national mechanical Standard 20153160333) used in the experiment of the embodiment of the invention mainly comprises the following components: polyhexamethylene biguanide, poloxamer, disodium ethylene diamine tetraacetate, hydroxypropyl methylcellulose, propylene glycol, sodium hyaluronate, boric acid, disodium hydrogen phosphate, sodium chloride, potassium chloride and water for injection, wherein the pH value is 6.8-7.8, and the osmotic pressure is 260 mOsm/Kg.
According to the experimental results, the protein elution rate of the reducing instrument matched with the Haichang water feeling nursing liquid (national mechanical Standard of record 20153160333) on the Rongchang safe, high-quality and smooth eye double-week polished hydrogel contact lens can reach 91.4%, and the protein elution rate of the Rongchang water feeling nursing liquid (national mechanical Standard of record 20153160333) for soaking the Rongchang safe, high-quality and smooth eye double-week polished hydrogel contact lens is only 2.9%, so that the effect of efficient cleaning and reduction on the Rongchang safe, high-quality and smooth eye double-week polished hydrogel contact lens can be achieved by starting the reducing instrument matched with the Haichang water feeling nursing liquid (national mechanical Standard of record 20153160333).
It should be noted that, in the experimental process described in the embodiment of the present invention, the acetonitrile-trifluoroacetic acid solution is used to perform vibration extraction on the protein, so that the protein attached to the lens can be separated from the lens, that is, the protein is dissolved in the protein extract, the light absorption value of the protein extract solution is detected by the micro ultraviolet spectrophotometer, and then the protein content is calculated according to the protein concentration detection standard curve.
The protein concentration detection standard curve needs to be established manually, and the establishment of the protein concentration detection standard curve specifically comprises the following steps:
preparing a 4mg/ml protein solution, carrying out multiple dilution on the protein solution, testing the light absorption value and the protein concentration of the protein solution for multiple times, recording the average value of the light absorption value obtained by the test, recording the average value of the protein concentration obtained by the test, see the following table 3-1, making a standard curve according to the average value of the light absorption value and the average value of the protein concentration, and if the linear deviation degree exceeds the range of +/-10%, considering that the standard data are invalid and needing to be redone;
the function expression of the protein concentration detection standard curve is as follows:
y＝2.64033436x-0.001002579，
wherein y represents the measured protein concentration value, and x represents the light absorption value of the protein at 280 nm; fitting a curve based on actual test data, the degree of agreement between the test data and the fitting function, using a quantity R related to the correlation coefficient2To evaluate, R2The closer the value is1, the higher the degree of agreement, the closer to 0, the lower the degree of agreement, in one case R is calculated2＝0.999999835。
TABLE 3-1 dilution table for protein solutions by multiple ratio
Example 4:
an experiment is carried out according to the detection method in the embodiment 2, so as to detect the elution rate of the reduction instrument matched with the care solution on the protein adsorbed on the surface and in the oxygen permeation holes of the double-circumference polished hydrogel contact lens in the embodiment, and the experimental steps are as follows:
1. eye Secret bi-weekly contact lenses (national institutes of record 20173220364, LOT: DF00068544) are thrown by the eyes for 8 hours in two weeks, and the lens materials are as follows: hydroxyethyl methacrylate, ethylene glycol dimethacrylate, methacrylic acid, an ultraviolet light absorber, an initiator and a coloring agent, and the water content is as follows: 38 percent;
2. after the human eyes wear the glasses for 8 hours, taking down the left and right glasses, respectively putting the left and right glasses into a 1.5ml centrifuge tube, adding 1ml acetonitrile-trifluoroacetic acid solution into the centrifuge tube, vibrating for 30 minutes, testing the lysozyme amount in the centrifuge tube to be 0.446mg and 0.449mg respectively, and measuring that the average adsorbed protein amount of the glasses worn for 1 day (8 hours) is 0.447mg, namely considering that the original protein amount of the glasses is 0.447 mg;
3. repeating the step 1, and wearing the same person for 8 hours by twice-week Eye Secret bi-weekly contact lenses (national institutes of record 20173220364, LOT: DF00068544) to obtain two pieces of corneal contact lenses with protein on the surfaces;
4. taking a piece of corneal contact lens in the step 3, placing the corneal contact lens in 1ml of Haichang water sensory-nursing solution for soaking for 10 minutes, then placing the corneal contact lens in a centrifugal tube of 1.5ml, adding 1ml of acetonitrile-trifluoroacetic acid solution into the centrifugal tube, vibrating for 30 minutes, and testing the lysozyme amount in the centrifugal tube to be 0.434 mg;
5. placing the other corneal contact lens in the step 3 in a cleaning tank of a reduction instrument, adding 1ml of Haichang water feeling care solution, starting the reduction instrument to clean for 3 minutes, wherein the voltage at two ends of a measuring probe in the cleaning process is 4.77V, and the current is 3.82mA (the voltage at two ends of the measuring probe in the cleaning process can be 0.4V-4.9V, and the current is 0.4-4mA), placing the corneal contact lens into a 1.5ml centrifuge tube after the cleaning process is finished, adding 1ml of acetonitrile-trifluoroacetic acid solution into the centrifuge tube, and testing the lysozyme amount in the centrifuge tube to be 0.038mg after vibrating for 30 minutes;
6. calculating the elution rate of the reduction instrument matched with the care solution on the protein adsorbed on the surface of the double-circumference polished hydrogel contact lens and in the oxygen permeation hole:
similarly, the protein elution rate for soaking a two-week-cast hydrogel contact lens with the conditioning solution was calculated to be equal to (1-0.434mg/0.447mg) 100% to 2.9%.
According to the experimental results, the protein elution rate of the reducing instrument matched with the care solution on the Haichang biweekly hydrogel-casting contact lens can reach 91.5%, and the protein elution rate of the Haichang biweekly hydrogel-casting contact lens soaked by the care solution can reach only 2.9%, so that the reducing instrument matched with the care solution on the Haichang biweekly hydrogel-casting contact lens can achieve the effect of efficient clean reduction.
Example 5:
an experiment is carried out according to the detection method in the embodiment 2, so as to detect the elution rate of the reduction instrument matched with the care solution on the protein adsorbed on the surface and in the oxygen permeation holes of the double-circumference polished hydrogel contact lens in the embodiment, and the experimental steps are as follows:
1. the human eyes wear Bausch's doctor Lun to throw "Ticon" Cosmetic dispersible Soft Contact Lens (national institutional approval 20163220090, LOT: CQ7229) for 8 hours, the Lens material: hefilcon A, water content: 42%;
2. after the human eyes are worn for 8 hours, taking the left and right spectacle lenses and respectively putting the spectacle lenses into a 1.5ml centrifugal tube, adding 1ml acetonitrile-trifluoroacetic acid solution into the centrifugal tube, testing the lysozyme amount in the centrifugal tube after vibrating for 30 minutes, wherein the lysozyme amount is 0.421mg and 0.423mg respectively, and measuring the average adsorbed protein amount of the spectacle lens worn for 1 day (8 hours) to be 0.422mg, namely considering the original protein amount of the spectacle lens to be 0.422 mg;
3. repeating the step 1, and wearing the doctor's Royal for 8 hours by double-week polishing of ' Ticon ' Cosmetic dispersible Soft Contact lenses (national institutes of academic record 20163220090, LOT: CQ7229) to obtain two pieces of corneal Contact lenses with protein on the surfaces;
4. taking a piece of corneal contact lens in the step 3, placing the corneal contact lens in 1ml of Haichang water sensory-nursing solution for soaking for 10 minutes, then placing the corneal contact lens in a centrifugal tube of 1.5ml, adding 1ml of acetonitrile-trifluoroacetic acid solution into the centrifugal tube, vibrating for 30 minutes, and testing the lysozyme amount in the centrifugal tube to be 0.416 mg;
5. placing the other corneal contact lens in the step 3 in a cleaning tank of a reduction instrument, adding 1ml of Haichang water feeling care solution, starting the reduction instrument to clean for 3 minutes, wherein the voltage at two ends of a measuring probe in cleaning is 4.9V, and the current is 4mA (the voltage at two ends of the measuring probe in cleaning can be 0.4V-4.9V, and the current is 0.4-4mA), placing the corneal contact lens into a 1.5ml centrifuge tube after cleaning, adding 1ml of acetonitrile-trifluoroacetic acid solution into the centrifuge tube, and testing the lysozyme amount in the centrifuge tube to be 0.033mg after vibrating for 30 minutes;
6. calculating the elution rate of the reduction instrument matched with the care solution on the protein adsorbed on the surface of the double-circumference polished hydrogel contact lens and in the oxygen permeation hole:
similarly, the rate of protein elution from the surface of a two-week-polished hydrogel contact lens soaked with the solution was calculated to be equal to (1-0.416mg/0.422mg) × 100% — 1.4%.
According to the experimental results, the protein elution rate of the reduction instrument matched with the Haichang water feeling care solution on the Bausch Loran two-week-polished hydrogel contact lens can reach 92.2%, and the protein elution rate of the reduction instrument matched with the Haichang water feeling care solution for soaking the Bausch Loran two-week-polished hydrogel contact lens is only 1.4%, so that the reduction instrument matched with the Haichang water feeling care solution can achieve the effect of efficient cleaning and reduction on the Bausch Loran two-week-polished hydrogel contact lens.
By combining the examples 2-5, it can be found that the reduction instrument of the present invention, in combination with a care solution, can effectively remove proteins from double-circumference polished hydrogel soft hydrophilic contact lenses of various large brands.
Example 6:
in order to detect the cleaning effect of the reduction method on the double-circumference polished hydrogel contact lens, the invention also provides a detection method for the reduction effect of the double-circumference polished hydrogel contact lens, the method mainly detects the degreasing effect of the lens, and the method comprises the following steps:
the effect of removing the surface grease of the double-circumference-polishing hydrogel contact lens by the cooperation of the detection reduction instrument and the care solution specifically comprises the following steps:
detecting the oil content on the surface of the double-circumference polished hydrogel contact lens with the surface adsorbed with oil,
and adding a care solution into a cleaning tank of the reduction instrument, placing the double-circumference polished hydrogel contact lens with the surface adsorbed with the grease into the care solution, starting the reduction instrument, detecting the grease content on the surface of the double-circumference polished hydrogel contact lens cleaned by the reduction instrument after cleaning is finished, and measuring the grease removal rate of the reduction instrument matched with the care solution on the double-circumference polished hydrogel contact lens.
Further, a beam can be emitted to the double-circumference polished hydrogel contact lens with the surface adsorbed with the grease through a fluorescence spectrophotometer, and a fluorescence intensity value of an emission spectrum is recorded, wherein the fluorescence intensity value corresponds to the grease content on the surface of the double-circumference polished hydrogel contact lens;
the method for removing and detecting the grease on the surface of the lens in the embodiment is used for carrying out a detection experiment, and the experiment steps are as follows:
1. selecting a double-circumference polished lens: qiangsheng-an-good-looking and moistening eye (Qiangsheng-an-good-looking)
2Brand Contact Lenses) two-week disposable hydrogelSoft hydrophilic contact lenses (national instruments are imported 20193162150, LOT No.: L530C2) material: etafilcon a hydrogel, bluish;
2. preparing an artificial tear: phosphatidyl choline-0.0005 mg/ml, cholesterol-0.0018 mg/ml, lysozyme-1.9 mg/ml, BSA-0.2mg/ml, g-globulin-0.1 mg/ml, Nacl-9mg/ml, GaCL 2.2H2O-0.25 mg/ml, Na2HPO 4.7H2O-0.28 mg/ml, the pH is adjusted to 7.8 using NaOH or Hcl;
3. the process of artificial adsorption of the sediment in vitro of the corneal contact lens: and (3) in a sterile environment, taking 2 Qiangsheng 'an' Youyouluding eye lenses, placing the lenses in the artificial tears prepared in the step (2), immersing the contact lenses in the artificial tears, and then placing the lenses in an incubator at 37 ℃ to replace the artificial tears every 24 hours. After 3 days of precipitate adsorption, the lenses were removed from the solution and tested after rinsing with saline.
4. Fluorescence spectrophotometer test: exciting wavelength is 360nm, emitting wavelength is 360nm, placing the cornea contact lens adsorbed by the precipitate in a fluorescence spectrophotometer, enabling the center of the lens to face to the light beam, and recording the fluorescence intensity value of the emission spectrum.
5. Washing the two lenses cultured in the step 3 by using physiological saline, placing the two lenses in a fluorescence spectrophotometer for testing, and recording the fluorescence intensity value of an emission spectrum to obtain the fluorescence intensity before cleaning of 35.2 and 35.6;
6. putting the lens with the fluorescence intensity of 35.2 measured in the step 5 into a cleaning tank of a reduction instrument, adding 1ml of multifunctional care solution (national mechanical Standard 20153160333) for the Haichang water to feel that the contact lens is cleaned for 30 minutes, taking out the lens, putting the lens into a fluorescence spectrophotometer for testing, and recording the fluorescence intensity value of an emission spectrum to obtain the cleaned fluorescence intensity of 8.3;
7. placing the lens with the fluorescence intensity of 35.6 measured in the step 5 into a cleaning tank of a reduction instrument, adding 1ml of multifunctional care solution (national mechanical Standard 20153160333) for the Haichang water to feel that the contact lens is soaked for 30 minutes, taking out the lens, placing the lens into a fluorescence spectrophotometer for testing, and recording the fluorescence intensity value of an emission spectrum to obtain the cleaned fluorescence intensity of 28.9;
8. calculating the degreasing rate of the lens cleaned by starting the reduction instrument in the step 6 and matching with the care solution as follows: (35.2-8.3)/35.2 ═ 76.4%;
and (3) calculating the degreasing rate of the lens soaked in the reduction instrument in the step (7) by matching with the care solution as follows:
(35.6-28.9)/35.6＝18.8％。
according to the experimental results, the removal rate of the surface grease of the hydrogel contact lens polished for two weeks of eyes can reach 76.4% by matching the reduction instrument with the care solution, and the removal rate of the surface grease of the lens removed by soaking the same lens in the cleaning tank of the reduction instrument in matching with the care solution is only 18.8%, so that the reduction instrument provided by the invention has the efficacy of removing lipid under the condition of starting cleaning by matching with the care solution.
In conclusion, the invention discloses a reduction method and a detection method of a double-circumference-polishing hydrogel contact lens, wherein the reduction method can eliminate protein, bacteria and lipid adsorbed on the surface and in an oxygen permeable hole of the double-circumference-polishing hydrogel contact lens, so that the reduction of the double-circumference-polishing hydrogel contact lens is realized, an effective cleaning mode aiming at the double-circumference-polishing hydrogel contact lens is provided for vast consumers, and the safety of the lens entering the eye is guaranteed; the reduction detection method provided by the invention can effectively verify the reduction effect of the reduction method on the double-circumference polished hydrogel contact lens, the detection method is used for detecting proteins and lipids which are invisible to naked eyes, and a detection result is provided for a consumer through a chemical experiment method. Experimental results of the embodiment of the invention show that the reduction instrument can be matched with care solution to carry out multi-directional cleaning reduction on the double-week polished hydrogel contact lens for protein removal, sterilization and degreasing.
In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example" or "some examples" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples described in this specification can be combined and combined by one skilled in the art.
While embodiments of the present invention have been shown and described above, it is to be understood that the above embodiments are exemplary and not to be construed as limiting the present invention, and that changes, modifications and variations may be made therein by those of ordinary skill in the art within the scope of the present invention.
Claims (9)
1. A reduction method of a bi-weekly-polished hydrogel contact lens is characterized by comprising the following steps:
adding a nursing solution and a double-circumference-polishing hydrogel contact lens into a cleaning tank of a reduction instrument, wherein two electrophoresis dissociation probes are oppositely arranged in the cleaning tank, starting the reduction instrument, and electrifying the two electrophoresis dissociation probes, wherein one electrophoresis dissociation probe is a positive electrode, the other electrophoresis dissociation probe is a negative electrode,
the protein attached to the surface of the double-circumference polished hydrogel contact lens is charged in the care solution, the charged protein moves towards the position of the electrode with the electric property opposite to that of the charged protein,
cl in the care solution-Moving towards the positive electrode, losing electrons and being oxidized into chlorine, the chlorine being dissolved in the care solution to generate hypochlorous acid, and the hypochlorous acid and the protein are subjected to redox reaction in the cleaning tank, so that the protein is degraded;
the hypochlorous acid generates self-oxidation-reduction reaction in the care solution to decompose hydrogen ions, chloride ions and oxygen, and absorbs electrons of functional proteins of microorganisms in the cleaning tank in the decomposition process so as to inactivate the functional proteins of the microorganisms, so that the microorganisms are killed.
2. A method of reducing a bi-weekly-throw hydrogel contact lens according to claim 1, further comprising:
grease attached to the surface of the double-circumference polished hydrogel contact lens is removed, wherein hydrogen ions with positive charges in the care solution move to the negative electrode and undergo a reduction reaction near the negative electrode to generate hydrogen, sodium ions and hydroxyl ions in the care solution generate sodium hydroxide, the grease on the surface of the double-circumference polished hydrogel contact lens and the sodium hydroxide undergo a saponification reaction, and the grease on the surface of the double-circumference polished hydrogel contact lens is removed.
3. A reduction method of a double-weekly-throw hydrogel contact lens according to claim 1,
the hypochlorous acid and the protein are subjected to redox reaction in the cleaning tank, and the protein is degraded, and the method specifically comprises the following steps:
reacting hypochlorous acid with a peptide chain forming a protein skeleton to obtain chloral formamide, hydrolyzing the peptide chain formed by the chloral formamide in a solution, breaking peptide bonds in the peptide chain, decomposing the peptide chain into micromolecule protein and amino acid, degrading the protein, and/or,
the hypochlorous acid reacts with the side chains of the peptide chains forming the protein skeleton, the side chains comprise lysine side chains, the hypochlorous acid reacts with the lysine side chains to form chloramines on the lysine side chains of the protein and the amino groups of the protein, the chloramines are decomposed to form organic molecule fragments [ 1,2 ] in a carbonyl form, peptide bonds on the lysine side chains are broken and decomposed into small-molecule proteins and amino acids, and the proteins are degraded.
4. A reduction method of a double-weekly-throw hydrogel contact lens according to claim 1,
the microorganisms comprise bacteria and viruses, the hypochlorous acid absorbs electrons of functional proteins on cell walls of the bacteria in a decomposition process, and the bacteria are inactivated;
the hypochlorous acid absorbs electrons of functional proteins on the outer shell of the virus in a decomposition process, and the virus is inactivated;
the hypochlorous acid has strong oxidizing property, permeates into the microbial cells, and generates an oxidation reaction with mycoprotein, nucleic acid and enzyme in the microbial cells so as to kill the microbes.
5. A reduction detection method of a bi-weekly-polished hydrogel contact lens is characterized by comprising the following steps: performing elution rate detection on the protein on the bi-weekly polished hydrogel contact lens reduced by the reduction method according to any one of claims 1 to 4, which specifically comprises the following steps:
detecting the protein content adsorbed on the surface of the double-circumference polished hydrogel soft hydrophilic contact lens to obtain the original protein content of the lens,
the double-circumference polished hydrogel soft hydrophilic contact lens is placed in a reduction instrument to be cleaned by matching with a care solution,
detecting the protein content of the double-circumference polished hydrogel soft hydrophilic contact lens cleaned by the reduction instrument to obtain the residual protein content of the cleaned lens,
and calculating and analyzing the residual protein amount of the cleaned lens and the original protein amount of the lens to obtain the elution rate of the reduction instrument matched with the care solution on the protein adsorbed on the surface and in the oxygen permeation holes of the double-circumference polished hydrogel soft hydrophilic contact lens.
6. The reduction detection method of a double-peripheral-throw hydrogel contact lens according to claim 5,
the double-circumference-polishing hydrogel soft hydrophilic contact lens is placed in a reduction instrument and is cleaned by matching with a care solution, and the method specifically comprises the following steps:
placing the double-circumference polished hydrogel soft hydrophilic contact lens in a cleaning tank of a reduction instrument to be cleaned for 3 minutes by matching with a care solution, wherein the voltage difference value between two electrophoresis dissociation probes in the cleaning tank is 0.4-4.9V and the current value is 0.4-4mA in the cleaning process;
detecting the protein content of the double-circumference polished hydrogel soft hydrophilic contact lens treated by the reduction instrument to obtain the residual protein content of the cleaned lens, wherein the method specifically comprises the following steps:
placing the double-circumference polished hydrogel soft hydrophilic contact lens treated by the reduction instrument into a protein extracting solution, vibrating to extract protein,
and detecting a protein concentration value in the protein extracting solution by using a micro ultraviolet spectrophotometer, and calculating according to the protein concentration value to obtain the residual protein amount of the cleaned lens.
7. The reduction detection method of a double-peripheral-throw hydrogel contact lens according to claim 6,
the protein extracting solution is formed by mixing 50 parts of acetonitrile, 50 parts of deionized water and 0.2 part of 100% trifluoroacetic acid;
calculating and analyzing the residual protein amount of the cleaned lens and the original protein amount of the lens to obtain the elution rate of the reduction instrument matched with the care solution on the protein adsorbed on the surface and in the oxygen permeation holes of the double-circumference polished hydrogel soft hydrophilic contact lens:
8. the reduction detection method of a double-weekly-throw hydrogel contact lens according to claim 5, further comprising:
the detection of the surface oil removal effect of the double-circumference polished hydrogel contact lens reduced by the reduction method of any one of claims 1 to 4 specifically comprises the following steps:
detecting the oil content on the surface of the double-circumference polished hydrogel contact lens with the surface adsorbed with oil,
and adding a care solution into a cleaning tank of the reduction instrument, placing the double-circumference polished hydrogel contact lens with the surface adsorbed with the grease into the care solution, starting the reduction instrument, detecting the grease content on the surface of the double-circumference polished hydrogel contact lens cleaned by the reduction instrument after the cleaning is finished, and measuring the grease removal rate of the reduction instrument matched with the care solution to the double-circumference polished hydrogel contact lens.
9. The reduction detection method of a double-circumference polished hydrogel contact lens according to claim 8,
emitting a light beam to the double-circumference polished hydrogel contact lens with the surface adsorbed with the grease through a fluorescence spectrophotometer, and recording a fluorescence intensity value of an emission spectrum, wherein the fluorescence intensity value corresponds to the grease content on the surface of the double-circumference polished hydrogel contact lens;
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011628648.5A CN114681652A (en) | 2020-12-31 | 2020-12-31 | Reduction method and detection method of double-circle polished hydrogel contact lens |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011628648.5A CN114681652A (en) | 2020-12-31 | 2020-12-31 | Reduction method and detection method of double-circle polished hydrogel contact lens |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114681652A true CN114681652A (en) | 2022-07-01 |
Family
ID=82134114
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011628648.5A Pending CN114681652A (en) | 2020-12-31 | 2020-12-31 | Reduction method and detection method of double-circle polished hydrogel contact lens |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114681652A (en) |
-
2020
- 2020-12-31 CN CN202011628648.5A patent/CN114681652A/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JPH07168145A (en) | Cleaning and disinfecting agent for contact lens | |
| GB2094992A (en) | Method and apparatus for sterilizing an object such as a contact lens | |
| JP3697294B2 (en) | Cleaning and disinfecting contact lenses | |
| CN1155900A (en) | Stable liquid enzyme compositions and methods of use in contact lens cleaning and disinfecting systems | |
| Franklin | Cleaning efficacy of single-purpose surfactant cleaners and multi-purpose solutions | |
| CN114681652A (en) | Reduction method and detection method of double-circle polished hydrogel contact lens | |
| EP4187312A1 (en) | Contact lens cleaner, and protein removal and sterilization method by means of electrophoresis dissociation | |
| US5449442A (en) | Cleaning and disinfecting method for contact lens | |
| CN1064705C (en) | Cleaning hydrophilic contact lenses by electrochemical means | |
| KR20050089980A (en) | Contact lens care compositions containing chitin derivatives | |
| AU638097B2 (en) | Electrolytic cleaning and disinfecting solution | |
| JPH09285529A (en) | Composition for disinfection for water bearing type soft contact lens, and its application | |
| CN114690448A (en) | Reduction method and detection method of quaternary polishing hydrogel contact lens | |
| CN114647100A (en) | Hard corneal contact lens cleaning device | |
| CN114681653A (en) | Protein removing and sterilizing method and detection method for monthly-polished hydrogel contact lens | |
| CN114690447A (en) | Protein removing and sterilizing method and detection method for half-year polished hydrogel contact lens | |
| US5225055A (en) | Method of cleaning and disinfecting contact lenses | |
| CN214751166U (en) | Hard corneal contact lens cleaning device | |
| JP2007140219A (en) | Agent for contact lens | |
| EP1557180A1 (en) | Disinfection method | |
| Christie et al. | Contemporary contact lens care products | |
| CN115793287A (en) | Reduction method, reduction effect detection method and reduction instrument for daily-cast corneal contact lens | |
| CN215082767U (en) | Protein removing and sterilizing device for hard corneal contact lens | |
| WO1991017469A1 (en) | Kit for contact lenses | |
| JPH08122717A (en) | Electrolytically ionized water disinfecting device and disinfecting and neutralizing method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |