CN114680241A

CN114680241A - Piglet concentrated feed containing biological fermentation protein and preparation method thereof

Info

Publication number: CN114680241A
Application number: CN202011612289.4A
Authority: CN
Inventors: 王洪燕
Original assignee: Tianjin Ruifu Agriculture And Animal Husbandry Technology Group Co ltd
Current assignee: Tianjin Ruifu Agriculture And Animal Husbandry Technology Group Co ltd
Priority date: 2020-12-30
Filing date: 2020-12-30
Publication date: 2022-07-01

Abstract

The invention relates to the technical field of pig feed, and particularly relates to a piglet concentrated feed containing biologically fermented protein and a preparation method thereof. The piglet concentrate comprises the following raw materials in percentage by weight: 65-67% of soybean meal, 9-11% of biological fermentation protein, 6-8% of rice bran meal, 3-5% of calcium hydrophosphate, 3-4% of stone powder, 3-4% of core material, 1-3% of DDGS, 1-3% of salt, 0.2-1% of sodium humate, 0.2-1% of soybean oil and 0.2-1% of choline chloride; wherein the core material comprises compound vitamins, compound trace elements and L-lysine hydrochloride according to the mass ratio of (0.15-0.45): (0.5-1.2): (1-1.8). The concentrated feed can promote the growth of bones and muscles of weaned pigs, improve the nutritional value and the utilization rate of the feed, improve the breeding intestinal tracts of livestock and poultry, enhance the resistance of bred animals to diseases and reduce the diarrhea rate.

Description

Piglet concentrated feed containing biological fermentation protein and preparation method thereof

Technical Field

The invention relates to the technical field of pig feed, in particular to piglet concentrated feed containing biological fermentation protein and a preparation method thereof.

Background

At present, domestic pigs are weaned at 21-28 days of advanced national piglets, the average body weight in 60 days can reach more than 25 kilograms, most farms in China are mainly in domestic scattered breeding, due to the limitations of piggeries, feeding management, feed conditions and the like, the domestic scattered breeding mostly adopts 30-45 days of weaning, the average body weight in 60 days is only about 16 kilograms, medium and large farms with high intensification degree are mostly weaning in 25-30 days, and the average body weight in 60 days is also only about 20 kilograms. The nursing piglets after weaning are easy to cause a plurality of headache problems of farmers, such as weight loss, low immunity, low survival rate and slow growth of the nursing piglets after weaning.

Therefore, many breeders mostly feed the piglets by concentrated feed or concentrated feed, the method can ensure the growth of the piglets and is labor-saving, but because the piglets are small, all organs of bodies are in a development stage, the requirements of the piglets cannot be met by only feeding the concentrated feed or the concentrated feed, the growth of the piglets can be slowed down by the shortage of trace elements, and because the nutrition collocation is unreasonable, a plurality of concentrated feed or concentrated feed are not digested and are discharged out of the bodies along with excrement, so that the feed waste is caused, the breeding cost is increased, the immunity of the piglets can be influenced, adverse consequences are caused, and the marketing time is prolonged. And some diseases often appear in the feeding process, and once some diseases are infected among the piglets, the consequences are very serious, and great loss is caused, so that how to improve the immunity of the piglets in the feed process is particularly important.

CN102987172A discloses a concentrated feed for weaned pigs, which comprises the following components: peeled soybean meal, enzymolysis plant small peptide, fish meal, choline chloride, an acidifier, a flavoring agent, a flavor enhancer, composite vitamins for porket, composite organic trace elements for porket, lysine, methionine, probiotics (lactobacillus, saccharomycetes and bacillus subtilis), calcium hydrophosphate, vegetable oil and stone powder. The feed is convenient to use, corn flour is added according to a recommended formula and is uniformly mixed to prepare the complete formula feed for the nursery pigs, the nutritional requirements of the nursery pigs are completely met, the growth potential of the piglets can be fully exerted, and the economic benefit is improved.

CN111657400A discloses a concentrated feed containing plant extracts for nursery pigs, which comprises the following components in parts by weight: 80.5-81.5% of soybean meal, 7.8-8.8% of rice bran meal, 2.7-3.7% of stone powder, 3.5-4.5% of calcium hydrophosphate, 1.5-2.5% of sodium chloride, 0.03-0.07% of compound vitamin, 0.13-0.17% of compound trace element, 0.95-1.05% of L-lysine hydrochloride, 0.03-0.07% of ethoxyquinoline and 0.15-0.25% of compound plant extract. The concentrated feed is used for nursery pigs, can improve the feed intake of the nursery pigs, reduces weaning stress, and has less diarrhea and fast growth. The concentrated feed can enable the weight to reach 30 kilograms as soon as possible, increase the subsequent growth potential, enable pigs to be slaughtered in advance and save the feed.

ZL201310591936.1 discloses a piglet feed containing Chinese herbal medicines, which is prepared by mixing corn flour, peanut meal, fish meal, corn protein powder, Chinese herbal medicine powder and a premix, wherein the premix is composed of pure organic acid, vitamins and various micro-minerals, can supply sufficient nutrition for the piglet growth, can sterilize and preserve, improve the anti-stress effect of animals, improve the immunity of the animals, promote the absorption of minerals, vitamins and nutrients, and improve the production performance of the animals.

Weaning and postweaning group transfer are all stressors for piglets, the digestive function, immune system and the like of the piglets in the nursing stage are not developed and perfected, if the piglets are improperly raised, the piglet mortality is increased, and great economic loss is caused, in the aspect of feed selection, as the digestive system of the piglets is not developed completely, digestible daily ration is provided for meeting the nutritional needs of the weaned piglets, and in the existing feed, 80% of the feed has the problem of poor utilization rate, so that the immunity of the piglets cannot be effectively improved, and the diarrhea rate of the piglets is reduced. Particularly, the diarrhea rate of weaned piglets is usually 20-30%, and the death rate is 2-4%. The diarrhea rate of the weaned piglets in part of pig farms can reach 70-80 percent, and the mortality rate can reach 15-20 percent. The growth speed, feed reward and disease resistance of the piglets after diarrhea are all reduced, and the problems of reducing the diarrhea rate of the piglets, improving the growth performance and ensuring the quality and quantity of the piglets become troublesome for practitioners in the broad animal husbandry industry under the background of reducing and limiting the resistance.

Disclosure of Invention

The invention aims to provide a piglet concentrated feed containing biological fermentation protein and a preparation method thereof. The concentrated feed can improve the growth performance of weaned pigs, reduce the diarrhea rate and improve the immunity of organisms.

In order to achieve the purpose, the invention adopts the following technical scheme:

a piglet concentrate feed containing biological fermentation protein is disclosed, wherein the piglet concentrate feed comprises the following raw materials in percentage by weight:

wherein the core material comprises vitamin complex, compound trace elements and L-lysine hydrochloride according to the mass ratio of (0.15-0.45): (0.5-1.2): (1-1.8).

According to the piglet concentrated feed formula containing the biological fermentation raw materials, the biological fermentation protein raw materials are added into the conventional feed raw materials, so that the piglet concentrated feed can serve as a single nutrition source to meet the nutritional requirements of piglets, and meanwhile, probiotics such as lactobacillus, bacillus and saccharomycetes for biological fermentation can regulate the intestinal micro-ecological environment of the piglets through synergistic effect, enhance the immunity and disease resistance of the piglets, prevent diarrhea of the piglets, promote the growth of bones and muscles of the piglets, promote the growth and development of the piglets, improve the production performance, improve the feed reward and the quality of livestock and poultry products.

In the invention, the compound vitamin is compound vitamin premix or compound vitamin commonly used in the field, the compound vitamin premix comprises vitamin A, vitamin E, vitamin B1, vitamin B2, vitamin B6, vitamin B12, vitamin C, vitamin D3, inositol, calcium pantothenate, folic acid, nicotinamide and biotin, in each 1 kg of the vitamin complex premix, the vitamin A contains 40-120 ten thousand IU, the vitamin D3 contains 50-90 ten thousand IU, the vitamin E contains at least 3 g, the vitamin B1 contains at least 1.5 g, the vitamin B2 contains at least 1 g, the vitamin B6 contains at least 2.4 g, the vitamin B12 contains at least 0.01 g, the vitamin C contains at least 36 g, the inositol contains at least 24 g, the calcium pantothenate contains at least 4 g, the folic acid contains at least 0.1 g, the nicotinamide contains at least 2 g, and the biotin contains at least 0.025 g. The vitamin complex premix can also be a commercially available vitamin complex premix. The compound vitamin can also be compound vitamin for piglets in CN102048030A, and can be compound vitamin composed of vitamin A acetate, vitamin D3, vitamin E, vitamin K, vitamin B1, vitamin B2, vitamin B6, vitamin B12, folic acid, biotin, calcium pantothenate and nicotinic acid.

The composite microelement is a composite microelement premix or a composite microelement commonly used in the field, the composite microelement premix comprises organic chelated mineral salts of microelements such as iron, copper, manganese, zinc, iodine, cobalt, magnesium, selenium and the like, wherein in every 1 kg of the composite microelement premix, 10-20 g of iron, 1-3.5 g of copper, at least 0.6 g of manganese, 4-7 g of zinc, at least 0.04 g of iodine, at least 0.125 g of cobalt, at least 7.5 g of magnesium and 0.1-0.3 g of selenium are contained. The compound microelement can be a compound microelement composed of ferrous sulfate, manganese sulfate, copper sulfate, potassium iodide, zinc sulfate, sodium selenite and cobalt sulfate.

As a preferable scheme, the piglet concentrate comprises the following raw materials in percentage by weight:

furthermore, the biological fermentation protein is prepared by adding bacterial liquid, enzyme liquid and water into a protein raw material for fermentation.

In the invention, the protein raw materials are bean pulp, cotton pulp and other protein raw materials.

The biological fermentation protein is obtained by fermenting protein raw materials such as bean pulp, cotton pulp and the like through microorganisms, a large amount of active peptide can be generated after the protein raw materials such as the bean pulp, the cotton pulp and the like are fermented through the microorganisms, and anti-nutritional factors in the protein raw materials are simultaneously degraded, so that the utilization rate of feed is improved, and the culture benefit is increased.

Further, the bacterial liquid is a mixed bacterial liquid of bacillus subtilis, bacillus licheniformis, saccharomycetes and lactic acid bacteria; the enzyme solution is a mixed enzyme of xylanase, protease, Aspergillus niger and mannanase.

The biological fermentation protein is added with a bacterial liquid in the fermentation process, wherein the bacterial liquid is a mixed bacterial liquid of bacillus subtilis, bacillus licheniformis, saccharomycetes and lactic acid bacteria. Wherein, the bacillus subtilis and the bacillus licheniformis can rapidly consume free oxygen in intestinal tracts, cause the hypoxia of the intestinal tracts, promote the growth of beneficial anaerobic bacteria, indirectly inhibit the growth of other pathogenic bacteria, synthesize enzymes such as alpha-amylase, protease, lipase and cellulase by themselves, play a role together with digestive enzymes in animal bodies in digestive tracts, synthesize a plurality of B vitamins such as vitamin B1, B2, B6 and nicotinic acid, and improve the activity of interferon and macrophage in animals; the yeast can provide sufficient nutrients for organisms, improve the immune system of a human body, quickly increase the resistance and improve constipation symptoms; the lactobacillus can regulate normal flora of gastrointestinal tract, maintain microecological balance, improve food digestibility and biological value, reduce serum cholesterol, control endotoxin, and inhibit growth and reproduction of putrefying bacteria and generation of putrefying product in intestinal tract.

The invention also provides a preparation method of the piglet concentrated feed, wherein the preparation method comprises the following steps:

1) preparation of biologically fermented proteins

Pulverizing protein raw materials, mixing, aging, adding bacterial solution, enzyme solution and water, and fermenting to obtain biologically fermented protein;

2) mixing material

Calcium hydrogen phosphate, stone powder, core material, DDGS, salt, sodium humate, soybean oil and choline chloride are crushed and sieved, and are uniformly mixed according to the weight percentage to obtain premix; and uniformly mixing the premix with the bean pulp, the rice bran meal and the biological fermentation protein prepared in the step 1), and packaging to obtain the piglet concentrated feed.

Further, in the step 1), the enzyme solution is a mixed enzyme of xylanase, protease, aspergillus niger and mannase, wherein the activities of the xylanase, the protease, the aspergillus niger and the mannase are 2 × 10¹⁰CFU/g。

Further, in the step 1), the bacterial liquid is a mixed bacterial liquid of bacillus subtilis, bacillus licheniformis, saccharomycetes and lactic acid bacteria, wherein the activity of the bacillus subtilis and the bacillus licheniformis is 1 × 10⁹CFU/g, 5X 10 activity of yeast and lactic acid bacteria⁸CFU/g。

Further, after the bacterial liquid, the enzyme liquid and the water are added in the step 1), the water content of the materials is controlled to reach 40%.

Further, in the step 1), the fermentation is carried out at 37 ℃ for 72 hours.

The invention further provides a use method of the piglet concentrate, wherein the use method is to mix the piglet concentrate with corn and bran, and the mass ratio of the concentrate to the corn and the bran is 1-3: 5.5-7.5: 1-2, preferably 2.5: 6.5: 1.

compared with the prior art, the invention has the following advantages:

the concentrated feed can promote the growth of bones and muscles of weaned pigs, improve the nutritional value and the utilization rate of the feed, improve the breeding intestinal tracts of livestock and poultry, enhance the resistance of bred animals to diseases and reduce the diarrhea rate.

Detailed Description

The following is a detailed description of the invention, which is intended to further illustrate the invention and not to limit it.

Example 1

The concentrated material consists of the following raw materials in percentage by weight:

wherein the core material comprises vitamin complex, compound trace elements and L-lysine hydrochloride according to a mass ratio of 0.3: 1: 1.5.

The compound vitamin is a compound vitamin premix which comprises vitamin A, vitamin E, vitamin B1, vitamin B2, vitamin B6, vitamin B12, vitamin C, vitamin D3, inositol, calcium pantothenate, folic acid, nicotinamide and biotin, wherein in every 1 kg of the compound vitamin premix, the vitamin A contains 50 ten thousand IU, the vitamin D3 contains 50 ten thousand IU, the vitamin E contains 8 g, the vitamin B1 contains 1.6 g, the vitamin B2 contains 1 g, the vitamin B6 contains 2.4 g, the vitamin B12 contains 0.01 g, the vitamin C contains 36 g, the inositol contains 24 g, the calcium pantothenate contains 4 g, the folic acid contains 0.1 g, the nicotinamide contains 2 g and the biotin contains 0.025 g.

The compound microelement is a compound microelement premix which comprises organic chelated mineral salts of microelements such as iron, copper, manganese, zinc, iodine, cobalt, magnesium, selenium and the like, wherein in every 1 kg of the compound microelement premix, 10 g of iron, 1 g of copper, 0.8 g of manganese, 4 g of zinc, 0.04 g of iodine, 0.15 g of cobalt, 7.5 g of magnesium and 0.1 g of selenium are contained.

The preparation method comprises the following steps:

1) preparation of biologically fermented proteins

Respectively pulverizing protein materials (soybean meal and cotton meal) to 45 mesh, mixing, steam aging, adding bacterial liquid, enzyme liquid and water to make water content of material reach 40%, wherein the bacterial liquid is mixed bacterial liquid of Bacillus subtilis, Bacillus licheniformis, yeast and lactobacillus, and activity of Bacillus subtilis and Bacillus licheniformis is 1 × 10⁹CFU/g, 5X 10 activity of yeast and lactic acid bacteria⁸CFU/g; the enzyme solution is a mixed enzyme of xylanase, protease, Aspergillus niger and mannase, wherein the activities of the xylanase, the protease, the Aspergillus niger and the mannase are 2 multiplied by 10¹⁰CFU/g; then performing solid state fermentation for 72h at 37 ℃ to obtain biological fermentation protein;

2) mixing material

Calcium hydrogen phosphate, stone powder, core material, DDGS, salt, sodium humate, soybean oil and choline chloride are crushed and sieved by a sieve sheet of 2.5mm, and are uniformly mixed according to the weight percentage to obtain premix; uniformly mixing the premix with the bean pulp, the rice bran meal and the biological fermentation protein prepared in the step 1) according to the weight percentage, packaging, and adjusting the loading specification to 40 kg/bag to obtain the piglet concentrated feed.

Example 2

wherein the core material comprises vitamin complex, compound trace elements and L-lysine hydrochloride according to a mass ratio of 0.15: 1.2: 1.

The compound vitamin is a compound vitamin premix which comprises vitamin A, vitamin E, vitamin B1, vitamin B2, vitamin B6, vitamin B12, vitamin C, vitamin D3, inositol, calcium pantothenate, folic acid, nicotinamide and biotin, wherein in every 1 kg of the compound vitamin premix, the vitamin A contains 120 ten thousand IU, the vitamin D3 contains 90 ten thousand IU, the vitamin E contains 16 g, the vitamin B1 contains 2 g, the vitamin B2 contains 2 g, the vitamin B6 contains 4.4 g, the vitamin B12 contains 0.02 g, the vitamin C contains 70 g, the inositol contains 36 g, the calcium pantothenate contains 10 g, the folic acid contains 0.8 g, the nicotinamide contains 8 g and the biotin contains 0.05 g.

The compound microelement is a compound microelement premix which comprises organic chelated mineral salts of microelements such as iron, copper, manganese, zinc, iodine, cobalt, magnesium, selenium and the like, wherein in every 1 kg of the compound microelement premix, the iron content is 20 g, the copper content is 2.2 g, the manganese content is 0.9 g, the zinc content is 6 g, the iodine content is 0.12 g, the cobalt content is 1.75 g, the magnesium content is 10.5 g, and the selenium content is 0.3 g.

The preparation method comprises the following steps: the same as example 1 except that the protein materials (soybean meal, cotton meal) were respectively pulverized to 30 mesh in step 1).

Example 3

wherein the core material comprises vitamin complex, compound trace elements and L-lysine hydrochloride in a mass ratio of 0.45: 0.5: 1.8.

The compound vitamin is a compound vitamin premix which comprises vitamin A, vitamin E, vitamin B1, vitamin B2, vitamin B6, vitamin B12, vitamin C, vitamin D3, inositol, calcium pantothenate, folic acid, nicotinamide and biotin, wherein in every 1 kg of the compound vitamin premix, the vitamin A contains 40 ten thousand IU, the vitamin D3 contains 60 ten thousand IU, the vitamin E contains 9 g, the vitamin B1 contains 1.9 g, the vitamin B2 contains 1.6 g, the vitamin B6 contains 2.8 g, the vitamin B12 contains 0.01 g, the vitamin C contains 44 g, the inositol contains 27 g, the calcium pantothenate contains 6 g, the folic acid contains 0.4 g, the nicotinamide contains 6 g and the biotin contains 0.023 g.

The compound microelement is a compound microelement premix which comprises organic chelated mineral salts of microelements such as iron, copper, manganese, zinc, iodine, cobalt, magnesium, selenium and the like, wherein in every 1 kg of the compound microelement premix, 18 g of iron, 2 g of copper, 0.6 g of manganese, 7 g of zinc, 0.09 g of iodine, 0.125 g of cobalt, 8.2 g of magnesium and 0.2 g of selenium are contained.

The preparation method comprises the following steps: the same as example 1 except that the protein materials (soybean meal, cottonseed meal) were respectively pulverized to 60 mesh in step 1).

Example 4

wherein the core material comprises vitamin complex, compound trace elements and L-lysine hydrochloride in a mass ratio of 0.2: 0.7: 1.2.

The compound vitamin is a compound vitamin premix which comprises vitamin A, vitamin E, vitamin B1, vitamin B2, vitamin B6, vitamin B12, vitamin C, vitamin D3, inositol, calcium pantothenate, folic acid, nicotinamide and biotin, wherein in every 1 kg of the compound vitamin premix, the vitamin A contains 60 ten thousand IU, the vitamin D3 contains 70 ten thousand IU, the vitamin E contains 10 g, the vitamin B1 contains 1.5 g, the vitamin B2 contains 2 g, the vitamin B6 contains 3.6 g, the vitamin B12 contains 0.012 g, the vitamin C contains 53 g, the inositol contains 30 g, the calcium pantothenate contains 7 g, the folic acid contains 0.5 g, the nicotinamide contains 5 g and the biotin contains 0.035 g.

The compound microelement is a compound microelement premix which comprises organic chelated mineral salts of microelements such as iron, copper, manganese, zinc, iodine, cobalt, magnesium, selenium and the like, wherein in every 1 kg of the compound microelement premix, the iron content is 20 g, the copper content is 3 g, the manganese content is 1.2 g, the zinc content is 6.5 g, the iodine content is 0.13 g, the cobalt content is 0.15 g, the magnesium content is 9.1 g, and the selenium content is 0.2 g.

The preparation method comprises the following steps: the same as example 1 except that the protein materials (soybean meal, cottonseed meal) were respectively pulverized to 50 mesh in step 1).

Example 5

wherein the core material comprises vitamin complex, compound trace elements and L-lysine hydrochloride according to a mass ratio of 0.35: 0.9: 1.3.

The compound vitamin is CN102048030A, the compound vitamin for the piglets prepared in example 1.

The compound microelement is a compound microelement premix which comprises organic chelated mineral salts of microelements such as iron, copper, manganese, zinc, iodine, cobalt, magnesium, selenium and the like, wherein in every 1 kg of the compound microelement premix, 19 g of iron, 3.5 g of copper, 1 g of manganese, 4.9 g of zinc, 0.1 g of iodine, 0.125 g of cobalt, 10 g of magnesium and 0.1 g of selenium are contained.

The preparation method comprises the following steps: the same as example 1, except that the protein materials (soybean meal, cottonseed meal) were respectively pulverized to 55 mesh in step 1).

Comparative example 1

The formula and the preparation process of the concentrated material are the same as those of the example 1, and the difference from the example 1 is that the formula of the concentrated material does not contain biological fermentation protein, and the content of the soybean meal is 76.284%.

Comparative example 2

The formula and the preparation process of the concentrate are the same as those of example 1, and different from example 1, the concentrate formula does not contain choline chloride, and the content of the sodium humate is 0.710%.

Comparative example 3

The formula and preparation process of the concentrate are the same as those of example 1, and the difference from example 1 is that xylanase is not contained in the enzyme liquid used for preparing the biological fermentation protein.

Test example 1

The experimental example investigates the influence of the piglet concentrated feed containing the biological fermentation protein and a certain brand piglet concentrated feed in the market on the growth performance of piglets.

The experimental piglets are 200 long three-element non-hybrid piglets which are equivalent in age, weight and male-female ratio and weaned in the same batch, and are randomly divided into two groups, namely an experimental group and a control group, wherein the experimental group is fed with the concentrated feed, the corn and the bran of the embodiment 1 of the invention, the control group is fed with the concentrated feed, the corn and the bran of a certain brand of piglets on the market, and the mass ratio of the concentrated feed, the corn and the bran of the experimental group to the control group is 22: 68: 10. the weight was weighed on an empty stomach in the morning at the beginning of the test and at the end of the test, respectively.

The experiment adopts the nipple type drinking bowl to drink water freely, the trough is an automatic trough independently designed for a pig farm, the daily work of sanitation, disinfection, epidemic prevention and the like is implemented according to the operation regulation formulated by the pig farm, and the experiment is carried out in a semi-open single-row piglet nursing house.

The measurement items include test body weight, test-end body weight, average weight gain, average daily food consumption, and the like. The test results are shown in table 1:

TABLE 1 influence of concentrate feed for piglets containing biofermented proteins on growth performance of piglets

Item	Test group	Control group
			Number of test heads (head)	100	100
Initial weight of head (kg)	20.92±0.93	24.08±1.34
			Average head weight (kg)	108.85±1.10	109.35±1.77
Average head weight gain (kg)	87.93±1.13	85.27±1.30
			Feed intake (kg)	244.01±1.33	255.23±1.50
Cost of feed per pig (Yuan)	964.6	1018.5
			Meat ratio of materials	2.75±1.18	2.89±1.49
Daily gain (kg)	0.98	0.952
			Profit per pig	886.6	802.5

The data in the table show that the average weight gain and daily gain of each piglet of the test group are superior to those of the piglet of the control group, and the profit of each piglet of the test group is obviously higher than that of the piglet of the control group, which indicates that compared with the conventional feed formula, the piglet concentrated feed formula containing the biological fermentation raw materials has obvious effects on enhancing the growth performance of the piglet and improving the breeding benefit.

Test example 2

1. Materials and methods

1.1 test materials

The test animals were 25-day-old weaned piglets (Du X long X large, 21-day weaning).

1.2 design of the experiment

The test is carried out in a pig farm for one life, and in the test period of 35d, 96 healthy piglets (male and female halves) with the average weight of 7.8kg and the age of 25 days, namely Du multiplied by long multiplied by big are selected in the test and randomly divided into 4 groups, each group has 6 repetitions, and each repetition has 4 pigs. The test groups were fed with the concentrate, corn and bran of example 1, and the control group 1, control group 2 and control group 3 were fed with the concentrate, corn and bran of comparative example 1, comparative example 2 and comparative example 3, respectively, at a mass ratio of 2.5: 6.5: 1. feeding and feeding are carried out independently for each repetition, the reasonable temperature (26-28 ℃) of the pigsty is controlled, and food and water are taken freely.

1.3 measurement index and method

1.3.1 growth Performance

Piglets in each group were weighed on an empty stomach and initial weight, final weight, feed consumption during the trial and diarrhea were recorded for each group. Calculating the average daily gain, the average daily feed intake, the feed-to-feed ratio and the diarrhea rate according to the feed consumption, the time and the like, wherein the calculation formula is as follows:

average Daily Gain (ADG) ═ end-initial weight/day of experiment

Average Daily Food Intake (ADFI) ═ amount of feed (feed-remainder)/(number of animals x days of the experiment)

Material to weight ratio (F/G) total feed intake/total weight gain

Diarrhea rate (%) [ number of piglets diarrhea heads/test day × number of piglets heads ] × 100

1.3.2 serum immunization

On the day of the test, 5mL of blood is collected from the anterior jugular vein, the blood is kept still for 15-20min, centrifugation is carried out for 15min at 3000-3500r/min, upper serum is taken, and the content of IgA is detected by using an ELISA kit.

1.4 data statistics and analysis

The experimental data were initially processed with Excel2010, single factor analysis of variance was performed using SPSS19.0 software, and significance of differences between groups was tested using Duncan's multiple comparisons, with the results being expressed as "mean ± standard deviation", with P < 0.05 being significant.

2. Results

2.1 Effect of different concentrates on growth Performance of weaned piglets

The results are shown in Table 2:

TABLE 2 Effect of different concentrates on growth Performance of weaned piglets

	Test group	Control group 1	Control group 2	Control group 3
					Initial weight (kg)	7.82±0.08	7.79±0.12	7.81±0.15	7.80±0.22
Terminal weight (kg)	25.41±0.13^b	21.26±0.19^a	22.62±0.23^a	21.58±0.31^a
					Average daily gain (g)	446.83±5.23^b	402.41±7.08^a	401.87±6.12^a	403.15±5.17^a
Average daily food intake (g)	59.34±4.31^b	671.28±5.73^a	673.38±6.28^a	677.51±5.19^a
					Material to weight ratio	1.33±0.03^a	1.67±0.06^b	1.68±0.05^b	1.68±0.07^b
Diarrhea Rate (%)	0.12±0.12^a	12.68±1.06^b	12.71±1.11^b	13.01±1.27^b

Note: the data in the same row indicates significant difference (P < 0.05) by different lower case letters, and the data in the same row indicates insignificant difference (P > 0.05) by the same letter or no letter.

As can be seen from the results in Table 1, the end and average daily gain of the piglets in the experimental group was significantly higher than that in the control group (P < 0.05); the feed-weight ratio and the diarrhea rate of the piglets in the test group are both obviously lower than those in each control group (P is less than 0.05).

2.2 Effect of different concentrates on serum immune index of weaned pig

The results are shown in Table 3:

TABLE 3 influence of different concentrates on serum immune index of weaned piglets

Group of	IgA	IgG	IgM
				Test group	1.67^b	1.72^b	1.74^b
Control group 1	0.96^a	1.13^a	1.06^a
				Control group 2	0.98^a	1.21^a	1.14^a
Control group 3	1.02^a	1.28^a	1.16^a

Note: the data in the same column indicates significant difference (P < 0.05) in different lower case letters, and the same letter or no letter indicates insignificant difference (P > 0.05).

As can be seen from the results in Table 2, the levels of IgA, IgG and IgM in the sera of the piglets in the test group were significantly increased (P < 0.05) compared to the respective control groups.

The major immunoglobulins in serum are IgA, IgG and IgM. IgA is primarily involved in mucosal local immunity; IgG primarily mediates anti-infective immunity; IgM is generally a protective antibody, and is the antibody which appears earliest in the primary humoral immune response, and has a strong bactericidal function. The immunoglobulin plays an important role in the process of mediating humoral immunity and is an important index for evaluating the immune function. The increase of IgA, IgG and IgM in reasonable range indicates that the level of antibody in animal body is high, which can help organism resist the invasion of external material better and reflect the improvement of organism immunity function to a certain extent.

The test results show that the concentrated feed can improve the growth performance of weaned pigs, reduce the diarrhea rate and improve the immunity of organisms.

The above tests were also carried out on concentrates prepared in other examples of the invention, which gave similar results.

Claims

1. The piglet concentrate feed containing the biological fermentation protein is characterized by comprising the following raw materials in percentage by weight:

2. The piglet concentrate according to claim 1, wherein the piglet concentrate comprises the following raw materials in percentage by weight:

3. the piglet concentrate according to claim 1 or 2, wherein the bio-fermented protein is prepared by adding a bacterial solution, an enzyme solution and water into a protein raw material for fermentation.

4. The piglet concentrate according to claim 3, wherein the bacterial liquid is a mixed bacterial liquid of bacillus subtilis, bacillus licheniformis, yeast and lactic acid bacteria; the enzyme solution is a mixed enzyme of xylanase, protease, Aspergillus niger and mannanase.

5. A method for preparing the piglet concentrate according to any one of claims 1 to 4, which comprises the following steps:

1) preparation of biologically fermented proteins

Crushing the protein raw material, uniformly mixing, curing, adding a bacterial liquid, an enzyme liquid and water, and fermenting to obtain the biological fermentation protein;

2) mixing material

6. The method according to claim 5, wherein in step 1), the enzyme solution is a mixture of xylanase, protease, Aspergillus niger and mannanase, wherein the activities of xylanase, protease, Aspergillus niger and mannanase are 2 x 10¹⁰CFU/g。

7. The method according to claim 5, wherein in step 1), the bacterial liquid is a mixture of Bacillus subtilis, Bacillus licheniformis, yeast and lactic acid bacteria, wherein the activity of Bacillus subtilis and Bacillus licheniformis is 1 x 10⁹CFU/g, 5X 10 activity of yeast and lactic acid bacteria⁸CFU/g。

8. The method according to claim 5, wherein the water content of the material is controlled to 40% after the bacterial liquid, the enzyme liquid and the water are added in the step 1).

9. The method according to claim 5, wherein the fermentation in step 1) is carried out at 37 ℃ for 72 hours.

10. The use method of the piglet concentrate according to any one of claims 1 to 4, characterized in that the piglet concentrate is mixed with corn and bran for use, wherein the mass ratio of the concentrate to the corn and the bran is as follows: 1-3: 5.5-7.5: 1-2, preferably 2.5: 6.5: 1.