CN114680077A - Method for preparing rat model for inhibiting hypertrophic scar by mechanical pressure - Google Patents
Method for preparing rat model for inhibiting hypertrophic scar by mechanical pressure Download PDFInfo
- Publication number
- CN114680077A CN114680077A CN202210039088.2A CN202210039088A CN114680077A CN 114680077 A CN114680077 A CN 114680077A CN 202210039088 A CN202210039088 A CN 202210039088A CN 114680077 A CN114680077 A CN 114680077A
- Authority
- CN
- China
- Prior art keywords
- elastic sleeve
- rat
- scar
- pressure
- wound surface
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 231100000241 scar Toxicity 0.000 title claims abstract description 39
- 238000000034 method Methods 0.000 title claims abstract description 20
- 230000001969 hypertrophic effect Effects 0.000 title claims abstract description 15
- 238000011552 rat model Methods 0.000 title claims abstract description 13
- 230000002401 inhibitory effect Effects 0.000 title abstract description 10
- 208000027418 Wounds and injury Diseases 0.000 claims abstract description 13
- 239000002184 metal Substances 0.000 claims abstract description 13
- 238000001514 detection method Methods 0.000 claims abstract description 9
- 206010072170 Skin wound Diseases 0.000 claims abstract description 3
- 230000002093 peripheral effect Effects 0.000 claims abstract description 3
- 208000032544 Cicatrix Diseases 0.000 claims description 5
- 229920002334 Spandex Polymers 0.000 claims description 5
- 230000005764 inhibitory process Effects 0.000 claims description 5
- 230000037387 scars Effects 0.000 claims description 5
- 239000004759 spandex Substances 0.000 claims description 5
- 239000004677 Nylon Substances 0.000 claims description 3
- 229920001778 nylon Polymers 0.000 claims description 3
- 238000009434 installation Methods 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims 1
- 230000037390 scarring Effects 0.000 claims 1
- 238000012795 verification Methods 0.000 abstract description 4
- 238000012360 testing method Methods 0.000 abstract description 3
- 241000700159 Rattus Species 0.000 description 19
- 238000010171 animal model Methods 0.000 description 7
- 206010052428 Wound Diseases 0.000 description 6
- 239000002390 adhesive tape Substances 0.000 description 3
- 229920006052 Chinlon® Polymers 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 238000002560 therapeutic procedure Methods 0.000 description 2
- 230000029663 wound healing Effects 0.000 description 2
- 241000581650 Ivesia Species 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009530 blood pressure measurement Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000036573 scar formation Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000012453 sprague-dawley rat model Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/02—Breeding vertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention relates to a method for preparing a rat model for inhibiting hypertrophic scar by mechanical pressure, which comprises the following steps: (1) selecting a rat with a full-layer skin wound surface in the middle of the dorsal side of a rat tail, fixing the tail of the rat on a metal ring, wherein the wound surface is positioned on an arc-shaped central shaft of the metal ring, and drawing the wound surface by using the metal ring as a traction source until the wound surface is healed to form a scar; (2) designing and cutting the elastic sleeve according to the peripheral diameter and the diameter of the rat tail, implanting a pressure detection chip, installing the elastic sleeve at the scar of the rat, measuring the pressure value of the scar at regular time, ensuring that the pressure value of the scar is kept at 15-20 mm Hg, dismantling the elastic sleeve if the pressure value is increased, cutting and installing the elastic sleeve again at the scar of the rat tail, and ensuring that the pressure value generated by a new elastic sleeve is unchanged until the modeling is finished. The rat tail model prepared by the method disclosed by the invention can be used for easily obtaining the antibody for molecular level verification, and the objectivity of the test can be better ensured.
Description
Technical Field
The invention relates to the field of animal modeling, in particular to a method for preparing a rat model for inhibiting hypertrophic scar by mechanical pressure.
Background
The inhibition of human pathologic scars by pressure therapy has been widely accepted, but currently, no accepted animal model which can be verified by molecular biotechnology is used for basic research, the animal model is limited by antibody species, the animal model for inhibiting hypertrophic scars by pressure cannot be verified at a molecular level, and the model is more imperfect. In view of the foregoing, the present invention is specifically provided.
Disclosure of Invention
The invention provides a method for preparing a rat model for inhibiting hypertrophic scar by mechanical pressure, which comprises the following steps:
(1) selecting a rat with a full-layer skin wound surface in the middle of the dorsal side of a rat tail, fixing the tail of the rat on a metal ring, wherein the wound surface is positioned on an arc-shaped central shaft of the metal ring, and drawing the wound surface by using the metal ring as a traction source until the wound surface is healed to form a scar;
(2) designing and cutting the elastic sleeve according to the peripheral diameter and the diameter of the rat tail, implanting a pressure detection chip, installing the elastic sleeve at the scar of the rat, measuring the pressure value of the scar at regular time, ensuring that the pressure value of the scar is kept at 15-20 mm Hg, dismantling the elastic sleeve if the pressure value is increased, cutting and installing the elastic sleeve again at the scar of the rat tail, and ensuring that the pressure value generated by a new elastic sleeve is unchanged until the modeling is finished.
In some specific embodiments, the method prepares multiple rat models of pressure inhibition hypertrophic scar simultaneously, and each rat model receives the same pressure when the elastic sleeve is applied by cutting, designing and installing an implanted pressure detection chip.
In some specific embodiments, the method further comprises removing and adjusting the elastic sleeve in time when the pressure change of the elastic sleeve caused by the difference of the growth speed of the rat tail is detected during the step (2); meanwhile, during the installation of the elastic sleeve, the scar is dosed as required, the elastic sleeve is removed and dosed, and the elastic sleeve is reinstalled to continue pressurization after dosing.
In some specific embodiments, the time period of the drawing in the step (1) is 12 to 16 days; and (3) pressurizing the elastic sleeve in the step (2) for 6-8 days.
In some specific embodiments, the time period for said drawing of step (1) is 14 days; and (3) pressurizing the elastic sleeve in the step (2) for 7 days.
In some specific embodiments, the wound surface is 6 x 6 mm.
In some specific embodiments, the elastic sleeve comprises spandex and chinlon.
In some specific embodiments, the stretch slipcover is comprised of 28% spandex and 72% nylon.
Advantageous effects
Compared with the prior art, the method for preparing the rat model for inhibiting the hypertrophic scar by the mechanical pressure has at least the following beneficial effects:
(1) the rat is a typical model animal, the research is wide, the source of the antibody is rich, the molecular level verification of abundance can be developed, the correctness of the animal model is improved, and the limitation of inhibiting the hypertrophic scar animal model by the pressure therapy which is not used for the molecular level verification at present is broken through;
(2) the method utilizes the elastic sleeve to carry out pressure treatment on the scar, simulates the condition that the elastic coat inhibits the hypertrophic scar of the human body, and the obtained model is closer to the actual clinical treatment scheme.
(3) In the invention, a pressure detection chip is creatively implanted in the elastic sleeve in the model preparation process, and the elastic sleeve is detected and adjusted at regular time, so that the pressure value received by the scar is not changed although the mouse tail grows up in the whole modeling process, and the pressure values received by a plurality of rats are the same when a plurality of rat models are prepared simultaneously, thereby ensuring the objectivity of the test.
(4) The elastic sleeve prepared by the method has the same principle and material as the clinical elastic sleeve for inhibiting scars, is closer to the actual clinical treatment scheme, and a rat tail model can more easily obtain an antibody for molecular level verification and can better ensure the objectivity of the test.
Drawings
FIG. 1A is a schematic diagram of a rat animal model with hypertrophic scar inhibition by stress;
FIG. 1B is an animal model of scar formation induced by tractive force;
FIG. 1C is a FlexiForce manometry;
figure 1D is the appearance of a hypertrophic scar, and the appearance of the scar after 1 week of treatment with the elastic sleeve;
FIG. 1E shows the results of HE and Masson staining.
Detailed Description
Embodiments of the present invention will be described in detail below with reference to examples, but it will be understood by those skilled in the art that the following examples are only illustrative of the present invention and should not be construed as limiting the scope of the present invention. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products commercially available.
Example 1 construction method of rat model for inhibiting hypertrophic scar by mechanical pressure
1. Male Sprague Dawley (SD) rats, 6-8 weeks, weighing around 160-.
2. A metal ring with the diameter of 2cm is used as a traction force source, and the adhesive tapes are bound, so that the wound surface is positioned at the arc center of the metal ring, and the traction force is ensured.
3. The adhesive tapes are tightly bound due to the growth of the rat tail, so that the adhesive tapes with the binding metal rings are replaced at intervals of 3 days, and the wound healing condition and the edema of the rat tail are noticed.
4. Wound healing was modeled for about 14 days.
5. After the wound surface is healed, a rat tail elastic sleeve customized by 28% spandex and 72% chinlon is cut according to the circumference of the rat tail at different distances from the root of the rat tail, a Flexiforcepressure detection chip is implanted into the elastic sleeve, and a FlexiForce pressure measuring device is used for measuring pressure, so that the pressure value of the elastic sleeve after each cutting is the same, and the local pressure values of all rat tail scars are the same. The metal loop was continued to pull the scar to the end of the experiment (see fig. 1B, 1C and 1D).
6. After cutting out at every turn, install elasticity cover after, can adopt Flexiforce pressure detection device pressure measurement, carry out pressure detection every 24 hours simultaneously, along with the rat tail grows, when the pressure value increases, remove original elasticity cover, customize new elasticity cover again according to the size.
7. Pressurizing the elastic sleeve for 1 week, taking materials, removing the elastic sleeve to inject the medicine if medicine intervention is needed, and then continuing pressurizing the elastic sleeve. The pressure values were ensured to be maintained in the range of 15mm Hg to 20mm Hg throughout the pressurization process.
8. Models were constructed and HE and Masson staining was performed on scar sites at the rat tail (see figure 1E).
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.
Claims (8)
1. A method of making a rat model for mechanical stress inhibition of hypertrophic scarring, comprising:
(1) selecting a rat with a full-layer skin wound surface in the middle of the dorsal side of a rat tail, fixing the tail of the rat on a metal ring, wherein the wound surface is positioned on an arc-shaped central shaft of the metal ring, and drawing the wound surface by using the metal ring as a traction source until the wound surface is healed to form a scar;
(2) designing and cutting the elastic sleeve according to the peripheral diameter and the diameter of the rat tail, implanting a pressure detection chip, installing the elastic sleeve at the scar of the rat, measuring the pressure value of the scar at regular time, ensuring that the pressure value of the scar is kept at 15-20 mm Hg, dismantling the elastic sleeve if the pressure value is increased, cutting and installing the elastic sleeve again at the scar of the rat tail, and ensuring that the pressure value generated by a new elastic sleeve is unchanged until the modeling is finished.
2. The method according to claim 1, wherein a plurality of rat models with pressure inhibition hypertrophic scars are prepared simultaneously, and the implanted pressure detection chip is designed and installed by cutting so that each rat model receives the same pressure when the elastic sleeve is applied.
3. The method according to claim 1, further comprising removing and adjusting the elastic sleeve in time when a change in pressure of the elastic sleeve due to a difference in the growth rate of the rat tail is detected during step (2); meanwhile, during the installation of the elastic sleeve, the scar is dosed as required, the elastic sleeve is removed and dosed, and the elastic sleeve is reinstalled to continue pressurization after dosing.
4. The method according to claim 1, wherein the time period of the drawing in the step (1) is 12 to 16 days; and (3) pressurizing the elastic sleeve in the step (2) for 6-8 days.
5. The method of claim 4, wherein the time for said drawing of step (1) is 14 days; and (3) pressurizing the elastic sleeve in the step (2) for 7 days.
6. The method of claim 1, wherein the wound is 6 x 6 mm.
7. The method of claim 1, wherein the elastic sleeve comprises spandex and nylon.
8. The method of claim 7, wherein said elastic cuff is comprised of 28% spandex and 72% nylon.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210039088.2A CN114680077A (en) | 2022-01-13 | 2022-01-13 | Method for preparing rat model for inhibiting hypertrophic scar by mechanical pressure |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210039088.2A CN114680077A (en) | 2022-01-13 | 2022-01-13 | Method for preparing rat model for inhibiting hypertrophic scar by mechanical pressure |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114680077A true CN114680077A (en) | 2022-07-01 |
Family
ID=82136852
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210039088.2A Pending CN114680077A (en) | 2022-01-13 | 2022-01-13 | Method for preparing rat model for inhibiting hypertrophic scar by mechanical pressure |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114680077A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003022184A2 (en) * | 2001-09-07 | 2003-03-20 | Michel Guillot | Orthosis for scar tissue treatment |
| CN106880418A (en) * | 2017-02-24 | 2017-06-23 | 中山大学附属第医院 | Devices and methods therefor for studying the effect of pressure therapy hyperplastic scar and mechanism |
| US20190373863A1 (en) * | 2017-01-31 | 2019-12-12 | The Board Of Regents Of The University Of Oklahoma | Animal wound model and methods of use |
| CN211797092U (en) * | 2020-01-17 | 2020-10-30 | 吉林大学 | Inflatable neck scar pressure elastic sleeve |
| CN212165771U (en) * | 2020-11-03 | 2020-12-18 | 中山大学附属第一医院 | Device for detecting effect and mechanism of pressure treatment hypertrophic scar |
-
2022
- 2022-01-13 CN CN202210039088.2A patent/CN114680077A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003022184A2 (en) * | 2001-09-07 | 2003-03-20 | Michel Guillot | Orthosis for scar tissue treatment |
| CN1578649A (en) * | 2001-09-07 | 2005-02-09 | 米歇尔·吉洛特 | Orthosis for scar tissue treatment |
| US20190373863A1 (en) * | 2017-01-31 | 2019-12-12 | The Board Of Regents Of The University Of Oklahoma | Animal wound model and methods of use |
| CN106880418A (en) * | 2017-02-24 | 2017-06-23 | 中山大学附属第医院 | Devices and methods therefor for studying the effect of pressure therapy hyperplastic scar and mechanism |
| CN211797092U (en) * | 2020-01-17 | 2020-10-30 | 吉林大学 | Inflatable neck scar pressure elastic sleeve |
| CN212165771U (en) * | 2020-11-03 | 2020-12-18 | 中山大学附属第一医院 | Device for detecting effect and mechanism of pressure treatment hypertrophic scar |
Non-Patent Citations (4)
| Title |
|---|
| 刘宁 等: "《体外构建3D细胞压力培养模型研究压力对增生性瘢痕成纤维细胞的作用》", 《中华损伤与修复杂志(电子版)》 * |
| 徐祥文 等: "《改良的大鼠鼠尾增生性瘢痕动物模型制备方法》", 《组织工程与重建外科杂志》 * |
| 朱冰 等: "《新编临床外科诊疗学》", 31 March 2018, 吉林科学技术出版社 * |
| 葛萍: "《新入职护士应知应会手册》", 31 August 2020, 科学技术文献出版 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Geissmann | Evolution of communication in gibbons (Hylobatidae) | |
| DE69019120D1 (en) | ARTIFICIAL LAWN, FLORGARN FOR AN ARTIFICIAL LAWN, AND METHOD FOR PRODUCING SUCH A LAWN. | |
| DE3855525T2 (en) | Use of a protein-bound polysaccharide For the manufacture of a medicament for the treatment of AIDS. | |
| ATE526897T1 (en) | SET OF ELEMENTS FOR PRODUCING A DENTAL PROSTHESIS, SYSTEM FOR PRODUCING A DENTAL PROSTHESIS OR A SET OF ELEMENTS AND CORRESPONDING MANUFACTURING METHOD | |
| ZA200900444B (en) | Thin film multilocular structure comprising collagen, material for tissue regeneration containing the same and method for producing the same | |
| DE502005005202D1 (en) | ZIRCONOXIDE BASED DENTAL IMPLANT AND METHOD FOR PRODUCING A DENTAL IMPLANT | |
| DE69524679T2 (en) | Use rapamycin for the manufacture of a medicament for the bowing and treatment of hyperproliferative vascular diseases, possibly in combination with mycophenolic acid | |
| WO2002032339A1 (en) | Ready-made customized impression tray for fabricating and fitting dentures | |
| CN114680077A (en) | Method for preparing rat model for inhibiting hypertrophic scar by mechanical pressure | |
| CN106976240A (en) | The design and preparation method of a kind of customization stabilizing brace after flexor tendon injury in hand reparation | |
| CN110115652A (en) | A kind of 3 D-printing protector and its Method of printing | |
| CN112992312B (en) | Qualified monitoring method and system for spinal cord injury rehabilitation training | |
| US20160354095A1 (en) | Surgical accessory for use in implanting medical device | |
| CN106860913A (en) | The application of antler polypeptide, tooth implant and its surface treatment method | |
| Schendel | Nasal Symmetry After Primary Cleft Lip Repair: Comparison Between Delaire Cheilorhinoplasty and Modified Rotation-Advancement: Discussion | |
| Döppler et al. | Gating related activity in a syringeal muscle allows the reconstruction of zebra finches songs | |
| WO2008010057A3 (en) | Pre-adjusted self-aligner and method | |
| EP0938875A2 (en) | Dental prosthesis support and method for its application | |
| Sanders et al. | Influence of prior activity on residual limb volume and shape measured using plaster casting: results from individuals with trans-tibial limb loss | |
| RU2734569C2 (en) | Method for setting artificial teeth in immediate prostheses in removing the entire group of anterior teeth | |
| RU2722800C2 (en) | Method of making individual silicone templates in damaged teeth | |
| CN111938848B (en) | Air gun mould taking method without gum line removal | |
| Sharma et al. | Comparative Evaluation of Dentures Fabricated Using Different Impression Materials for Border Molding | |
| Chung et al. | Precision VISSTA: Bring-Your-Own-Device (BYOD) mHealth Data for Precision Health. | |
| CN104548080A (en) | Preparation method of individualized vaccine preparation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20220701 |