CN114672408A

CN114672408A - Kit, reaction device and detection method

Info

Publication number: CN114672408A
Application number: CN202011550000.0A
Authority: CN
Inventors: 占伟; 王柳艺; 唐宇
Original assignee: XIAMEN ZEESAN BIOTECH CO Ltd
Current assignee: XIAMEN ZEESAN BIOTECH CO Ltd
Priority date: 2020-12-24
Filing date: 2020-12-24
Publication date: 2022-06-28

Abstract

The disclosure relates to a kit, a reaction device and a detection method. The kit comprises: a housing (11) having an internal chamber (A); at least one temperature conducting member (12) located at least partially within the interior chamber (A) and configured to derive energy from an external heat source (22) to form a temperature conducting region within the interior chamber (A); at least one fluid channel (13) which is positioned in the internal chamber (A), passes through the at least one temperature conduction component (12) or is tightly attached to the surface of the at least one temperature conduction component (12), is configured for flowing reagent fluid and exchanges heat with the at least one temperature conduction component (12); wherein the at least one fluid channel (13) has at least one interface located at or through an outer wall of the housing (11). The embodiment of the disclosure can better control the transfer and temperature of the reagent fluid.

Description

Kit, reaction device and detection method

Technical Field

The disclosure relates to the field of biological detection, and in particular relates to a kit, a reaction device and a detection method.

Background

In the field of molecular diagnostics, it is often necessary to extract nucleic acids from a sample from a human body and then perform nucleic acid detection. The concentration of the extracted nucleic acid molecules is very low and is difficult to identify by the naked eye or an instrument. In the related art, the concentration of nucleic acid molecules is significantly increased before the related detection is performed, and the process of increasing the concentration of nucleic acid molecules is called nucleic acid amplification.

Common amplification methods include Polymerase Chain Reaction (PCR) amplification, isothermal amplification, and the like. These methods require that the sample nucleic acid and amplification reagents be placed together in a single container and subjected to biochemical reactions at one or more temperatures.

The detection after the nucleic acid amplification can adopt a fluorescent dye and a fluorescent probe method to carry out qualitative or quantitative detection on the nucleic acid through the intensity of an optical signal.

In some related art, universal PCR tubing or specialized consumables are used for amplification and detection.

Disclosure of Invention

Research shows that when a universal PCR pipe is adopted, the temperature change of a detection reagent in the PCR pipe only depends on the temperature change of a heating device, the change process is difficult to be carried out quickly, the PCR pipe and the heating device are not tightly attached, the heat exchange is slow, the temperature change rate and the actual temperature accuracy of a solution in the pipe are obviously influenced, and in addition, the PCR pipe limits the heating and detection modes and is difficult to be miniaturized and speeded; in adopting special consumptive material, need guarantee the laminating degree between special consumptive material and the relatively independent heater as far as possible to reduce heat conduction loss, this production degree of difficulty that leads to special consumptive material is great, improves heat exchange rate through increasing area of contact moreover, can make solution comparatively disperse, thereby reduce the sensitivity that detects, in addition, special consumptive material also needs the temperature variation through heating device to realize the amplification, and alternating temperature rate is comparatively limited.

In view of this, the embodiments of the present disclosure provide a kit, a reaction device and a detection method, which can better control the temperature of a reagent fluid.

In one aspect of the present disclosure, there is provided a kit comprising:

a housing having an interior chamber;

at least one temperature conducting member, at least partially located within the interior chamber, configured to receive energy from an external heat source to form a temperature conducting region within the interior chamber;

at least one fluid channel, which is located in the inner chamber, passes through the at least one temperature conduction component or clings to the surface of the at least one temperature conduction component, is configured to allow the reagent fluid to flow and exchange heat with the at least one temperature conduction component;

wherein the at least one fluid channel has at least one port located in or through the housing outer wall.

In some embodiments, the at least one temperature conducting component is closely attached to or exposed from at least one side wall of the housing, and the at least one side wall of the housing is configured to transfer heat provided by the external heat source to the at least one temperature conducting component by heat conduction.

In some embodiments, the at least one temperature conducting component comprises an electrical heating element configured to convert electrical energy from the external heat source into thermal energy.

In some embodiments, the at least one temperature conducting member includes a plurality of temperature conducting members configured to form a plurality of temperature conducting regions in the internal chamber, respectively, the plurality of temperature conducting regions having the same or different temperatures, and the at least one fluid passage sequentially passes through the plurality of temperature conducting members or sequentially abuts on surfaces of the plurality of temperature conducting members.

In some embodiments, the at least one interface comprises:

at least one fluid driving interface configured to connect a fluid driving element to enable the reagent fluid in the at least one fluid channel to flow in the temperature conduction region corresponding to each temperature conduction component under the driving of the fluid driving element, or stay in the temperature conduction region corresponding to at least one of the plurality of temperature conduction components.

In some embodiments, the at least one fluid drive interface comprises two fluid drive interfaces configured to drive the flow of reagent fluid back and forth within the at least one fluid channel.

In some embodiments, the at least one interface comprises:

a detection interface configured to connect a detection element to detect a reagent fluid in a fluid channel corresponding to a designated area in the internal chamber through the detection element.

In some embodiments, the designated area is located within the temperature conductive area or between adjacent temperature conductive areas.

In some embodiments, the at least one fluid channel comprises a plurality of fluid channels, the plurality of fluid channels being disposed side-by-side and not in communication with each other.

In some embodiments, the at least one interface comprises:

a sample injection interface configured to receive a reagent fluid or to connect to a sample injection element.

In some embodiments, the reagent fluid comprises sample nucleic acids and amplification reagents, nucleic acid extraction reagents, or nucleic acid purification reagents.

In one aspect of the present disclosure, there is provided a reaction apparatus comprising:

a receiving part configured to receive the aforementioned reagent cartridge;

an energizing component coupled to the receiving component and configured to provide energy to at least one temperature conducting component in the cartridge as an external heat source for the cartridge.

In some embodiments, the at least one temperature conducting member comprises a plurality of temperature conducting members, each abutting against at least one side wall of the housing; the energy supply part comprises a plurality of heating blocks, and the receiving part is configured to enable the plurality of heating blocks to be respectively clung to the at least one side wall in a state of receiving the reagent kit and to be in one-to-one correspondence with the plurality of temperature conduction parts, so that the plurality of heating blocks transmit heat to the plurality of temperature conduction parts through the at least one side wall in a heat conduction mode.

In some embodiments, the at least one temperature conductive member includes a plurality of temperature conductive members respectively exposed on at least one side wall of the housing; the energy supply part comprises a plurality of heating blocks, and the receiving part is configured to enable the plurality of heating blocks to be tightly attached to the exposed parts of the plurality of temperature conduction parts in a one-to-one correspondence mode under the state that the reagent kit is received, so that the plurality of heating blocks can transmit heat to the plurality of temperature conduction parts respectively in a heat conduction mode.

In some embodiments, the at least one temperature conducting means comprises an electric heating element, and the energizing means comprises a power connector configured to electrically connect with the electric heating element in a state where the receiving means receives the reagent cartridge.

In some embodiments, the reaction apparatus further comprises:

a controller in signal connection with the energizing component configured to control the energizing component or the at least one temperature conducting component via the energizing component to control the temperature of a plurality of temperature conducting areas respectively formed in the interior chamber by the at least one temperature conducting component.

In some embodiments, at least one fluidic channel in the kit comprises at least one fluidic drive interface; the reaction apparatus further comprises:

a fluid drive element in signal connection with the controller configured to connect with the at least one fluid drive interface.

In some embodiments, at least one fluidic channel in the kit comprises a detection interface; the reaction apparatus further comprises:

a detection element in signal connection with the controller configured to be connected with the detection interface.

In some embodiments, at least one fluidic channel in the kit comprises a sample injection interface; the reaction apparatus further comprises:

a sample injection element in signal connection with the controller configured to connect with the sample injection interface.

In one aspect of the present disclosure, a detection method based on the aforementioned reaction device is provided, which includes:

adding a reagent fluid to at least one fluid channel of the aforementioned kit;

receiving the reagent cartridge by a receiving part of the reaction device;

connecting a fluid driving element of the reaction device to at least one fluid driving interface of the at least one fluid channel to drive a flow of reagent fluid within the at least one fluid channel by the fluid driving element;

providing energy to at least one temperature conducting means in the cartridge by an energy providing means of the reaction device to effect a temperature change of the reagent fluid;

connecting a detection element of the reaction device to a detection interface of the at least one fluid channel to detect the reagent fluid through the detection element.

Therefore, according to the embodiment of the present disclosure, by providing the temperature conducting component capable of obtaining energy from the external heat source in the inner cavity of the housing of the reagent cartridge, and making the fluid channel which is located in the inner cavity of the housing and has the interface pass through the temperature conducting component or cling to the surface of the temperature conducting component, the reagent fluid flowing or staying in the fluid channel can perform good heat exchange with the temperature conducting component. By integrating the fluid channel and the temperature sensing means in the cartridge, temperature changes of the reagent fluid can be effectively controlled in the cartridge by means of the temperature conducting means.

Drawings

The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments of the disclosure and together with the description, serve to explain the principles of the disclosure.

The present disclosure may be more clearly understood from the following detailed description, taken with reference to the accompanying drawings, in which:

FIG. 1 is a schematic diagram of the configuration of a kit according to the present disclosure in connection with an external heat source;

FIG. 2 is a schematic diagram of a configuration for connecting to an external heat source and for connecting to a detection element according to some embodiments of kits of the present disclosure;

FIG. 3 is a block schematic diagram of some embodiments of a reaction apparatus according to the present disclosure;

fig. 4 is a schematic flow diagram of some embodiments of a reaction device-based detection method according to the present disclosure.

It should be understood that the dimensions of the various parts shown in the figures are not drawn to scale. Further, the same or similar reference numerals denote the same or similar components.

Detailed Description

Various exemplary embodiments of the present disclosure will now be described in detail with reference to the accompanying drawings. The description of the exemplary embodiments is merely illustrative and is in no way intended to limit the disclosure, its application, or uses. The present disclosure may be embodied in many different forms and is not limited to the embodiments described herein. These embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the disclosure to those skilled in the art. It should be noted that: the relative arrangement of parts and steps, the composition of materials, numerical expressions and numerical values set forth in these embodiments are to be construed as merely illustrative, and not as limitative, unless specifically stated otherwise.

The use of "first," "second," and similar terms in this disclosure is not intended to indicate any order, quantity, or importance, but rather are used to distinguish one element from another. The word "comprising" or "comprises", and the like, means that the element preceding the word covers the element listed after the word, and does not exclude the possibility that other elements are also covered. "upper", "lower", "left", "right", and the like are used merely to indicate relative positional relationships, and when the absolute position of the object being described is changed, the relative positional relationships may also be changed accordingly.

In the present disclosure, when a specific device is described as being located between a first device and a second device, there may or may not be intervening devices between the specific device and the first device or the second device. When a particular device is described as being coupled to other devices, that particular device may be directly coupled to the other devices without intervening devices or may be directly coupled to the other devices with intervening devices.

All terms (including technical or scientific terms) used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure belongs unless specifically defined otherwise. It will be further understood that terms, such as those defined in commonly used dictionaries, should be interpreted as having a meaning that is consistent with their meaning in the context of the relevant art and will not be interpreted in an idealized or overly formal sense unless expressly so defined herein.

Techniques, methods, and apparatus known to those of ordinary skill in the relevant art may not be discussed in detail but are intended to be part of the specification where appropriate.

Fig. 1 is a schematic diagram of a configuration for interfacing with an external heat source according to some embodiments of kits of the present disclosure. FIG. 2 is a schematic diagram of a configuration for connecting to an external heat source and for connecting to a detection element according to some embodiments of kits of the present disclosure.

Referring to fig. 1 and 2, in some embodiments, a kit comprises: a housing 11, at least one temperature conducting component 12 and at least one fluid channel 13. The housing 11 has an internal chamber a. At least one temperature conducting member 12, at least partially located within the interior chamber a, is configured to receive energy from an external heat source 22 to form a temperature conducting region within the interior chamber a. In some embodiments, the temperature conductive component may comprise a metal or alloy material having a relatively high rate of thermal conductivity, such as pure copper, an aluminum alloy, or a composite of copper and aluminum alloy. In other embodiments, other materials may be used for the interface of the temperature conducting member and the fluid channel, such as heat conducting silicone grease, heat conducting silicone rubber, heat conducting silicone oil, and the like. In other embodiments, the temperature conductive member may be filled with a liquid heat transfer medium as a filler to provide a uniform and stable temperature field for heating the reagent fluid in the fluid channel.

At least one fluid channel 13 is located in the internal chamber a, and passes through the at least one temperature conducting member 12 or abuts against the surface of the at least one temperature conducting member 12, and is configured to allow a reagent fluid to flow and to exchange heat with the at least one temperature conducting member 12. The reagent fluid herein may include a liquid such as a sample nucleic acid and an amplification reagent (a general amplification reagent or a fluorescent amplification reagent), a nucleic acid extraction reagent or a nucleic acid purification reagent. In other embodiments, the reagent fluid may be in other forms, such as gas.

At least one fluid channel 13 has at least one port located on or through the outer wall of the housing 11. A part of the fluid passage 13 may be parallel to the side wall of the housing 11 or may be inclined. For a plurality of fluid channels 13, the planes of the fluid channels 13 may be parallel or cross. The interface may include an end opening of the fluid channel and may also include an end opening of a bypass channel connected to the fluid channel. In fig. 2, the ports on both sides of the fluid channel 13 are open-ended fluid channel ports, and the port in the middle is open-ended bypass channel port. The end openings of the fluid channels or the end openings of the bypass channels may be increased or decreased as desired.

In this embodiment, a temperature conducting component capable of obtaining energy from an external heat source is arranged in the inner cavity of the shell of the reagent kit, and a fluid channel which is positioned in the inner cavity of the shell and is provided with an interface passes through the temperature conducting component or is tightly attached to the surface of the temperature conducting component. Thus, the reagent fluid flowing or staying in the fluid channel can realize good heat exchange with the temperature conduction component, and realize higher temperature change rate. This embodiment can effectively control the temperature change of the reagent fluid by using the temperature conducting component in the reagent cartridge by integrating the fluid channel and the temperature sensing component in the reagent cartridge.

In this embodiment, a space may be provided between the inner wall of the inner cavity of the housing and the temperature conducting member, and a heat insulating filler may also be provided, so that the temperature control of the temperature conducting region is more stable.

Referring to fig. 1, in some embodiments, the at least one fluid channel 13 includes a plurality of fluid channels 13, and the plurality of fluid channels 13 are disposed side by side and do not communicate with each other. This allows the kit to simultaneously detect multiple detection reagents. For example, in FIG. 1, the reagent kit comprises 8 fluid channels, each of which can accommodate a nucleic acid sample to be detected and amplification reagents. This effectively promotes the miniaturization and detection speed of the kit.

In fig. 1, at least one temperature conducting member 12 is closely attached to at least one side wall (for example, a lower side wall) of the housing 11, and the at least one side wall of the housing 11 is configured to transfer heat supplied from the external heat source 22 to the at least one temperature conducting member 12 by a heat conduction manner. Since the temperature conducting member 12 and the external heat source 22 are both closely attached to the lower side wall of the case, the efficiency of conducting heat from the external heat source 22 to the temperature conducting member 12 is improved. The temperature conductive member 12 may include a material having a high thermal conductivity rate, such as a metallic material, e.g., aluminum, copper, or silver.

In other embodiments, at least one temperature conduction component 12 may be exposed on at least one side wall of the housing 11, so that the external heat source 22 closely attached to the surface of the temperature conduction component 12 directly transfers heat to the temperature conduction component 12, thereby improving the efficiency of heat conduction.

In addition to being thermally conductive from an external heat source, in other embodiments, at least one temperature conductive member 12 may also include an electrical heating element. The electrocaloric elements may be configured to convert electrical energy from the external heat source 22 to thermal energy. The electrical heating element may comprise a resistive heating element or an electromagnetic heating element. The electrocaloric element, when connected to an external heat source 22, is capable of converting received electrical energy to thermal energy, thereby regulating the temperature of the reagent fluid within the fluid channel.

In some embodiments, the kit may include only one temperature conducting component to meet application scenarios where the requirement for temperature variation of the reagent is not high. Referring to fig. 1 and 2, in other embodiments, the at least one temperature conductive member 12 includes a plurality of temperature conductive members 12. The plurality of temperature conduction members 12 may respectively form a plurality of temperature conduction regions in the internal chamber a. At least one fluid channel 13 passes through the plurality of temperature conducting members 12 in sequence, or is closely attached to the surfaces of the plurality of temperature conducting members 12 in sequence.

In some embodiments, the temperatures of the plurality of temperature conductive regions are different. For example, the temperature of the plurality of temperature conduction regions is gradually increased along the extending direction of at least one fluid channel, and the reagent fluid in the fluid channel flows through the temperature conduction regions or stays in the temperature conduction regions to realize the temperature change of the reagent fluid, thereby meeting the process of gradually changing the temperature during the flowing process of the reagent fluid in the fluid channel.

In some embodiments, each temperature conductive region may be maintained at a respective calibration temperature. This embodiment may achieve a higher temperature change rate by circulating the reagent fluid within each of the different temperature conducting zones than by controlling the temperature change of the heating device. For example, by causing the reagent fluid to circulate more rapidly between different temperature conducting zones, to increase the temperature swing efficiency of the reagent fluid.

For sample nucleic acid and amplification reagents, more efficient nucleic acid amplification can be realized by rapid circulating movement of the reagent fluid between the temperature conduction regions, and the concentration of nucleic acid to be detected can be increased. In other embodiments, the temperatures of the multiple temperature conduction regions may be the same to meet the needs of other application scenarios.

In fig. 1, the plurality of fluid channels 13 may each sequentially pass through the plurality of temperature conductive members 12, or sequentially abut against the surfaces of the plurality of temperature conductive members 12, so that the reagent fluid in each fluid channel 13 flows through the same heat exchange condition as that of each temperature conductive member 12, thereby effectively reducing the difference of the test conditions of the reagent fluid in each fluid channel.

Referring to fig. 1, in some embodiments, at least one interface includes a sample injection interface 133. The sample injection interface 133 can be disposed at one end of the fluid channel 13 (e.g., the leftmost end of the fluid channel 13 in fig. 1). The sample injection port 133 can receive a reagent fluid injected by an operator, for example, by the operator holding a container containing a sample fluid, and adding the sample fluid into the sample injection port 133. In other embodiments, a sample injection element through which the injection operation of the reagent fluid is performed may also be connected to the sample injection interface 133. Referring to fig. 1, in some embodiments, the at least one interface includes at least one fluid drive interface 131. The at least one fluid drive interface 131 may be configured to connect to a fluid drive element 23, such as a peristaltic pump or the like. This enables the reagent fluid in the at least one fluid channel 13 to flow in the corresponding temperature conduction region of each temperature conduction component 12 under the driving of the fluid driving element 23; it is also possible to cause the reagent fluid in at least one of the fluid channels 13 to stay in the temperature conducting area corresponding to at least one of the plurality of temperature conducting members 12 under the driving of the fluid driving element 23.

In fig. 1, the at least one fluid drive interface 131 comprises two fluid drive interfaces 131 configured to drive the reagent fluid to flow back and forth within the at least one fluid channel 13. For example, a group of fluid driving interfaces 131 on the left side is connected with a peristaltic pump to drive the reagent fluid to flow from the left side to the right side, and the temperature change is realized when the reagent fluid passes through or stays in the temperature conduction areas corresponding to the three temperature conduction components of the fluid channel; the set of fluid drive ports 131 on the right side may also be connected to a peristaltic pump to drive reagent fluid from the right side back to the left side. The peristaltic pumps connected with the fluid driving interfaces 131 on the left side and the right side can realize the left-right reciprocating flow of the reagent fluid in the fluid channel, so as to realize the more rapid temperature change of the reagent fluid.

Referring to fig. 1 and 2, in some embodiments at least one interface may include a detection interface 132. The detection interface 132 can be coupled to a detection element 24, such as a fluorescence detection element for nucleic acids. This allows the reagent fluid in the fluid channel 13 corresponding to the designated area in the internal chamber a to be detected by the detection element 24. The designated area herein may be located within the temperature conductive area or between adjacent temperature conductive areas.

In fig. 2, the detection interface to which the detection element 24 is connected corresponds to a position between the temperature conductive areas corresponding to the two temperature conductive members 22 on the right side. In fig. 1, for a plurality of fluid channels 13, the detection interfaces 132 may be arranged in a plurality of groups, and each group of detection interfaces 132 may be used to detect reagent fluid between two adjacent temperature conductive regions.

The kit of the above embodiments can be used as a nucleic acid detection kit to realize nucleic acid detection. In other embodiments, the kit may be a sample pretreatment kit, a nucleic acid extraction kit, or a kit integrating sample pretreatment, nucleic acid extraction, and nucleic acid detection.

FIG. 3 is a block schematic diagram of some embodiments of reaction devices according to the present disclosure. Referring to fig. 3, in some embodiments, the reaction device includes a receiving component 21 and an energizing component 22. The receiving part 21 may be used to receive the reagent cartridge in the previous embodiment of the present disclosure. The energy supply part 22 is connected with the receiving part 21 and can be used as an external heat source 22 of the reagent kit to supply energy to at least one temperature conduction part 12 in the reagent kit.

In some embodiments, the receiving part 21 may be a rack or a platform on the reaction device in order to firmly place or support the reagent cartridge with or without reagent fluid. The at least one temperature conducting member 12 may be supplied with energy via an energy supply member 22 when the receiving member 21 receives the reagent cartridge.

Referring to fig. 1 and 2, in some embodiments, the at least one temperature conductive member 12 includes a plurality of temperature conductive members 12 each abutting at least one side wall of the housing 11. Accordingly, the energy supply component 22 may include a plurality of heating blocks. The receiving member 21 may be configured to allow the plurality of heating blocks to be respectively closely attached to the at least one side wall (for example, a lower side wall) in a state of receiving the reagent cartridge, and to correspond to the plurality of temperature conduction members 12 one by one. This enables the plurality of heating blocks to transfer heat by thermal conduction to the plurality of temperature conducting members 12 via the at least one side wall, respectively.

In fig. 1, the heating block may be an electric heating element, and is connected to a power source through a wire 221. In other embodiments, the heating block may also be a heat exchange element, and the temperature change of the heating block may be realized by other heat exchange media, such as water, refrigerant, and the like.

In other embodiments, the at least one temperature conductive member 12 includes a plurality of temperature conductive members 12, each exposed on at least one side wall of the housing 11. Accordingly, the energy supply unit 22 may also comprise a plurality of heating blocks. The receiving member 21 may closely contact the plurality of heating blocks to the exposed portions of the plurality of temperature conduction members 12 in a one-to-one correspondence in a state where the reagent cartridge is received, so that the plurality of heating blocks transfer heat to the plurality of temperature conduction members 12, respectively, by a heat conduction manner.

In the above embodiment, the heat conduction loss can be reduced to a greater extent by bringing the side wall of the temperature conduction member in the reagent cartridge into close contact with the heating block, or bringing the portion of the temperature conduction member exposed at the side wall of the reagent cartridge into close contact with the heating block. For the fluid channel, the temperature conduction component passes through or is tightly attached to the fluid channel, so that on the basis of not increasing the contact area of the fluid channel, high-efficiency heat conduction can be realized, and the detection sensitivity is increased.

In addition to being thermally conductive, in some embodiments, at least one temperature conductive member 12 includes an electrical heating element. The power supply part 22 includes a power connector configured to be electrically connected to the electric heating element in a state where the receiving part 21 receives the reagent cartridge.

Referring to fig. 3, in some embodiments, the reaction apparatus further comprises a controller 25. A controller 25 may be in signal communication with the energizing component 22 and configured to control the energizing component 22 to control the temperature of the plurality of temperature conducting areas respectively formed in the interior chamber a by the at least one temperature conducting component 12. In other embodiments, the controller 25 may also control the at least one temperature conducting member 12 via the energy supply member 22, for example, by controlling the temperature of each temperature conducting region by controlling the current applied to the temperature conducting member 12, thereby achieving self-heating temperature control of the temperature conducting member.

To flow or dwell reagent fluid within the fluid channels as desired, referring to fig. 1 and 2, in some embodiments, at least one fluid channel 13 in the cartridge includes at least one fluid drive interface 131. Correspondingly, in fig. 3, the reaction device further comprises a fluid driving element 23, for example a peristaltic pump. The drive element 23 is in signal communication with the controller 25 and may be configured to interface with the at least one fluid drive interface 131.

To facilitate testing of the cartridge, at least one fluid channel 13 in the cartridge includes a test interface 132. Accordingly, in some embodiments, the reaction device further comprises a detection element 24, such as a fluorescence detection element or the like. The detection element 24 is in signal communication with the controller 25 and is configured to interface with the detection interface 132. The detection element 24 is used to detect the reagent fluid in a specific region of the reagent cartridge via the detection interface 132.

In fig. 1-3, at least one fluid channel 13 in the cartridge includes a sample injection interface 133. The reaction device further comprises a sample injection element 26. The sample injection element 26 is in signal connection with the controller 25 and is configured to be in connection with the sample injection interface 133.

In some embodiments, the reaction device comprises both the receiving part 21 and the energy supply part 22, and also the driving element 23 and the detection element 24, which may facilitate the application of the aforementioned reagent cartridge to a greater extent.

Fig. 4 is a schematic flow diagram of some embodiments of a reaction device-based detection method according to the present disclosure. Referring to fig. 3 and 4, based on the reaction device of the foregoing embodiment, the embodiment of the present disclosure provides a corresponding detection method, including steps S1 to S5.

In step S1, reagent fluid is added to the at least one fluid channel 13 of the aforementioned reagent cartridge. For example, sample nucleic acid and amplification reagents are added to the fluid channel manually or through the sample injection element 26. For embodiments in which the kit includes multiple fluidic channels 13, multiple sample nucleic acids and amplification reagents may be added to each fluidic channel separately.

In step S2, the reagent cartridge is received by the receiving part 21 of the reaction apparatus. This process may be a process of carrying and holding the cartridge by a rack or platform on the reaction device.

In step S3, the fluid driving element 23 of the reaction device is connected to the at least one fluid driving interface 131 of the at least one fluid channel 13 to drive the reagent fluid to flow in the at least one fluid channel 13 through the fluid driving element 23. Referring to fig. 1 and 2, one or two fluid driving elements 23 may be disposed on the fluid driving interface 131 of the fluid channel, and the reagent fluid may flow between the respective temperature conduction regions or stay at a certain temperature conduction region by the driving action of the fluid driving elements 23.

As the fluid driven element 23 drives the reagent fluid to flow to one side of the fluid channel 13, the reagent fluid may be quickly returned to the other side of the fluid channel 13 by the fluid driven element 23 or another fluid driven element 23.

In step S4, at least one temperature conducting part 12 in the cartridge is energized by the energizing part 22 of the reaction device to effect a temperature change of the reagent fluid. The energy supply unit 22 may heat the temperature conducting unit 12 by heat conduction or power supply, so that the temperature conducting unit 12 can adjust the temperature of the reagent fluid flowing to the corresponding temperature conducting region.

In step S5, the detection element 24 of the reaction device is connected to the detection interface 132 of the at least one fluid channel 13 to detect the reagent fluid through the detection element 24.

In the above detection method, the timing relationship among steps S3, S4 and S5 is not strict, for example, steps S3 and S4 may be performed simultaneously, and step S5 may be performed during the execution of steps S3 and S4.

In the present specification, a plurality of embodiments are described in a progressive manner, the emphasis of each embodiment is different, and the same or similar parts between the embodiments are referred to each other. For the method embodiments, further details are not given here, since the whole and the steps involved can be referred to the partial description of the kit and reaction apparatus embodiments.

Thus, various embodiments of the present disclosure have been described in detail. Some details that are well known in the art have not been described in order to avoid obscuring the concepts of the present disclosure. It will be fully apparent to those skilled in the art from the foregoing description how to practice the presently disclosed embodiments.

Although some specific embodiments of the present disclosure have been described in detail by way of example, it should be understood by those skilled in the art that the foregoing examples are for purposes of illustration only and are not intended to limit the scope of the present disclosure. It will be understood by those skilled in the art that various changes may be made in the above embodiments or equivalents may be substituted for elements thereof without departing from the scope and spirit of the present disclosure. The scope of the present disclosure is defined by the appended claims.

Claims

1. A kit, comprising:

a housing (11) having an internal chamber (A);

at least one temperature conducting member (12) located at least partially within the interior chamber (A) and configured to derive energy from an external heat source (22) to form a temperature conducting region within the interior chamber (A);

at least one fluid channel (13) which is positioned in the internal chamber (A), passes through the at least one temperature conduction component (12) or is tightly attached to the surface of the at least one temperature conduction component (12), is configured for flowing reagent fluid and exchanges heat with the at least one temperature conduction component (12);

wherein the at least one fluid channel (13) has at least one interface located at or through an outer wall of the housing (11).

2. The kit according to claim 1, wherein the at least one temperature conducting part (12) is in close contact with or exposed at least one side wall of the housing (11), the at least one side wall of the housing (11) being configured to transfer heat provided by the external heat source (22) to the at least one temperature conducting part (12) by means of heat conduction.

3. The kit according to claim 1, wherein the at least one temperature conducting component (12) comprises an electrical heating element configured to convert electrical energy from the external heat source (22) into thermal energy.

4. The kit according to claim 1, wherein the at least one temperature conducting member (12) comprises a plurality of temperature conducting members (12) configured to form a plurality of temperature conducting areas in the internal chamber (a), respectively, the temperatures of the plurality of temperature conducting areas being the same or different, and the at least one fluid channel (13) passes through the plurality of temperature conducting members (12) in sequence or is in close contact with the surfaces of the plurality of temperature conducting members (12) in sequence.

5. The kit of claim 4, wherein the at least one interface comprises:

at least one fluid driving interface (131) configured to connect a fluid driving element (23) so as to enable the reagent fluid in the at least one fluid channel (13) to flow in the temperature conduction region corresponding to each temperature conduction component (12) under the driving of the fluid driving element (23) or stay in the temperature conduction region corresponding to at least one of the plurality of temperature conduction components (12).

6. The cartridge of claim 5, wherein the at least one fluidic drive interface (131) comprises two fluidic drive interfaces (131) configured to drive a reagent fluid to flow back and forth within the at least one fluidic channel (13).

7. The kit of claim 4, wherein the at least one interface comprises:

a detection interface (132) configured to connect a detection element (24) so as to detect, by the detection element (24), a reagent fluid in a fluid channel (13) corresponding to a specified region in the internal chamber (A).

8. The kit of claim 6, wherein the designated area is located within the temperature conductive area or between adjacent temperature conductive areas.

9. The kit according to claim 1, wherein the at least one fluid channel (13) comprises a plurality of fluid channels (13), the plurality of fluid channels (13) being arranged side by side and not communicating with each other.

10. The kit of claim 1, wherein the at least one interface comprises:

a sample injection interface (133) configured to receive a reagent fluid or to connect to a sample injection element (26).

11. The kit of claim 1, wherein the reagent fluid comprises sample nucleic acids and amplification, nucleic acid extraction, or nucleic acid purification reagents.

12. A reaction apparatus, comprising:

a receiving part (21) configured to receive the kit of any one of claims 1 to 11;

an energizing component (22) connected to the receiving component (21) and configured to energize at least one temperature conducting component (12) in the cartridge as an external heat source (22) of the cartridge.

13. The reactor according to claim 12, wherein the at least one temperature conducting member (12) comprises a plurality of temperature conducting members (12) respectively abutting against at least one side wall of the housing (11); the energy supply part (22) comprises a plurality of heating blocks, and the receiving part (21) is configured to enable the plurality of heating blocks to be respectively clung to the at least one side wall in a state of receiving the reagent kit and to be in one-to-one correspondence with the plurality of temperature conduction parts (12), so that the plurality of heating blocks transmit heat to the plurality of temperature conduction parts (12) through the at least one side wall in a heat conduction mode.

14. The reaction device according to claim 12, wherein the at least one temperature conductive member (12) includes a plurality of temperature conductive members (12) respectively exposed on at least one side wall of the housing (11); the energy supply part (22) comprises a plurality of heating blocks, and the receiving part (21) is configured to enable the plurality of heating blocks to be clung to the exposed parts of the plurality of temperature conduction parts (12) in a one-to-one correspondence mode under the state that the reagent kit is received, so that the plurality of heating blocks can respectively transfer heat to the plurality of temperature conduction parts (12) in a heat conduction mode.

15. The reaction device according to claim 12, wherein the at least one temperature conducting means (12) comprises an electric heating element and the energizing means (22) comprises a power connector configured to be electrically connected to the electric heating element in a state where the receiving means (21) receives the reagent cartridge.

16. The reaction device of claim 12, further comprising:

a controller (25) in signal connection with the energizing means (22) configured to control the energizing means (22) or the at least one temperature conducting means (12) via the energizing means (22) to control the temperature of a plurality of temperature conducting areas respectively formed in the inner chamber (A) by the at least one temperature conducting means (12).

17. A reaction device according to any of claims 12 to 16, wherein at least one fluid channel (13) in the cartridge comprises at least one fluid driving interface (131); the reaction apparatus further comprises:

a fluid drive element (23) in signal connection with the controller (25) configured to be connected with the at least one fluid drive interface (131).

18. The reaction device of claim 17, wherein at least one fluid channel (13) in the cartridge comprises a detection interface (132); the reaction apparatus further comprises:

a detection element (24) in signal connection with the controller (25) configured to be connected with the detection interface (132).

19. The reaction device of claim 17, wherein at least one fluid channel (13) in the cartridge comprises a sample injection interface (133); the reaction apparatus further comprises:

a sample injection element (26) in signal connection with the controller (25) configured to be connected with the sample injection interface (133).

20. A detection method based on the reaction device of claim 18, comprising:

adding a reagent fluid to at least one fluid channel (13) of a kit according to any of claims 1 to 11;

receiving the reagent cartridge by a receiving part (21) of the reaction device;

connecting a fluid driving element (23) of the reaction device to at least one fluid driving interface (131) of the at least one fluid channel (13) to drive a flow of reagent fluid within the at least one fluid channel (13) by the fluid driving element (23);

providing energy to at least one temperature conducting means (12) in the cartridge by an energy providing means (22) of the reaction device to effect a temperature change of the reagent fluid;

connecting a detection element (24) of the reaction device to a detection interface (132) of the at least one fluid channel (13) for detecting the reagent fluid by the detection element (24).