CN217542822U - Sample detection system - Google Patents
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- CN217542822U CN217542822U CN202220887773.6U CN202220887773U CN217542822U CN 217542822 U CN217542822 U CN 217542822U CN 202220887773 U CN202220887773 U CN 202220887773U CN 217542822 U CN217542822 U CN 217542822U
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Abstract
The utility model relates to a biochemical detection device, in particular to sample detection system. The utility model discloses a sample detection system, which comprises a liquid path system and a sample detection device; the fluid path system is configured to independently circulate reagents to at least two micro-channels of the sample detection device, and comprises a micro-fluid path plate, a reagent kit, a sample introduction device, a reagent selection valve, a flow channel selection valve and a drive pump; the sample detection device is configured to detect biochemical reactions of at least two samples in the at least two micro flow channels with selected reagents, and comprises an optical signal detection device and a micro flow channel device. The utility model discloses can detect two at least or two types of samples simultaneously, also can successively detect two at least or two types of samples, concrete detection mode can be according to different user demands and nimble adjustment.
Description
Technical Field
The utility model relates to a biochemistry detection device, in particular to sample detection system.
Background
The gene sequencing is to analyze the base sequence in DNA or RNA segment by certain technological means, the gene sequencing can analyze and determine the whole gene sequence from blood or saliva to predict the possibility of suffering from various diseases, and the gene sequencing technology can lock the individual pathological gene and prevent and treat in advance. In recent years, gene sequencing technology has been widely applied to various fields such as basic scientific research, clinical diagnosis, molecular breeding, judicial identification, and the like. Meanwhile, with the continuous progress of technology and the continuous reduction of cost, gene sequencing has increasingly entered medical institutions, third-party clinical medical examination laboratories and scientific research institutions.
The gene sequencing is realized based on a series of biochemical reactions (biochemical reactions) and detection and analysis of reaction results. Generally, biochemical reactions require specific devices and supporting platforms as aids; similarly, detection and analysis also need a corresponding device and a matched platform as assistance. The current mainstream biochemical reaction device consists of a reaction chip and a flow channel; the detection devices are generally classified into electrical detection devices and optical detection devices according to the difference of detection signals, and optical detection is dominant.
The main functional modules of the sequencing system comprise a reagent module, a liquid path control module, an optical module, a temperature control module, an electronic communication module and the like. In order to realize the function of gene sequencing, the modules need to be coordinated with each other, for example, the optical module needs to take a picture of an optical signal generated on the surface of the sequencing chip in real time, the liquid path control module needs to control the flow of a sequencing reagent into and out of the sequencing chip, the temperature control module needs to heat the sequencing chip in real time, and the electronic communication module needs to control the whole system.
In the sequencing system, a liquid path control module is responsible for conveying reaction reagents and cleaning reagents used in the sequencing process into a sequencing chip according to a specific time sequence. Because the reagents used in the sequencing process are various (up to more than ten reagents), and the reagent dosage is very large (up to hundreds of milliliters), in the traditional high-throughput sequencer, the volume occupied by the liquid path control module and the reagent module is very large, and the miniaturization and the portability of the sequencer are not facilitated.
CMOS image sensors, i.e., complementary metal oxide semiconductor image sensors, are optical sensors based on a platform of semiconductor manufacturing processes. Under the background of the internet of things era, an image sensor is an eye of an electronic product as a device for converting an optical signal into an electrical signal, and can provide machine vision for various applications. Due to its optical detection capability, CMOS image sensors can be used for optical analysis of biological or chemical substances, and are particularly suitable for optical signal detection in biochemical reactions.
Some miniaturized sequencers based on CMOS image sensors are available in the market at present, but a sequencing chip of the miniaturized sequencers only has one fluid channel, so that one chip can be only used for detecting one or one type of samples, the waste of the area of the chip can be caused in certain scenes, and the miniaturized sequencers are not beneficial to flexible use of customers.
SUMMERY OF THE UTILITY MODEL
In order to solve the above problem in the prior art, the utility model provides a sample detection system can detect two at least or two types of samples simultaneously, also can successively detect two at least or two types of samples, and concrete detection mode can be according to different user demands and nimble adjustment.
The utility model provides a sample detection system, which comprises a liquid path system and a sample detection device;
the fluid path system configured to independently circulate reagents to at least two microchannels of the sample detection device, the fluid path system comprising:
the microfluidic circuit board is connected with the sample detection device and comprises at least two input channels corresponding to the at least two microchannels, and each input channel is configured to convey a reagent to the corresponding microchannel;
a cartridge coupled to the microfluidic circuit board, the cartridge configured to store a reagent;
a sample introduction device coupled to the microfluidic circuit board, the sample introduction device configured to be able to enter the cartridge to contact one or more reagents, the one or more reagents being delivered to the microfluidic circuit board via the sample introduction device;
a reagent selection valve coupled to the microfluidic circuit board, the reagent selection valve configured to select a particular reagent from the one or more reagents delivered at a particular time;
a flow channel selector valve coupled to the microfluidic circuit board, the flow channel selector valve configured to deliver a selected reagent to one or more of the at least two input flow channels;
a drive pump coupled to the microfluidic circuit board, the drive pump configured to provide a driving force for the flow of reagents within the sample detection device and the microfluidic circuit board; and is
The sample detection device is configured to detect biochemical reactions of at least two samples in the at least two micro flow channels with a selected reagent, and comprises:
an optical signal detection device including a plurality of image sensor chip units;
and the micro-channel device is arranged on the upper surface of the optical signal detection device and is provided with at least two micro-channels, and each micro-channel corresponds to at least one image sensor chip unit.
In one embodiment of the present invention, the kit comprises a reaction and wash reagent zone configured to store reaction reagents and wash reagents.
In one embodiment of the present invention, the kit comprises a waste liquid recovery area, the microfluidic circuit board comprises at least two output channels corresponding to the at least two microchannels, and each output channel is configured to convey the reacted reagent from the corresponding microchannel to the waste liquid recovery area.
In one embodiment of the present invention, the sample injection device is connected to the sample injection device hole site of the microfluidic circuit board, the microfluidic circuit board comprises one or more reagent flow channels, and one or more reaction reagents and/or cleaning reagents are/is received from the reaction and cleaning reagent zone via the sample injection device is carried to the one or more reagent flow channels.
In one embodiment of the present invention, the sample feeding device comprises a liquid pipeline.
In one embodiment of the present invention, the reagent selection valve is connected to a reagent selection valve orifice of the microfluidic circuit board, the microfluidic circuit board includes a reagent selection flow channel, and the reagent selection valve is configured to select a specific reagent from the one or more reagent flow channels at a specific time to be carried to the reagent selection flow channel.
In an embodiment of the present invention, the flow channel selection valve is connected to a flow channel selection valve aperture of the microfluidic circuit board.
In one embodiment of the present invention, the driving pump is connected to the at least two output channels of the microfluidic circuit board.
The utility model discloses an embodiment, the liquid way system still includes pressure measurement, pressure measurement is configured to detect pressure in the liquid way system.
In an embodiment of the present invention, the micro flow channel device includes a housing, at least two reagent inlets and at least two reagent outlets are disposed on the housing, the at least two micro flow channels are formed between a lower surface of the housing and an upper surface of the optical signal detection device, and each micro flow channel corresponds to one reagent inlet and one reagent outlet;
each micro flow channel and the corresponding reagent flow-in port and reagent flow-out port are configured such that the selected reagent flows into the corresponding micro flow channel via the corresponding reagent flow-in port, reaches the surface of the corresponding image sensor chip unit, and flows out from the corresponding reagent flow-out port.
As described above, the utility model discloses a sample detection system has following beneficial effect:
the utility model discloses a liquid road system includes the miniflow liquid way board, and the miniflow liquid way board includes two at least input flow channels corresponding with two at least miniflow channels of sample detection device, and every input flow channel is configured to carry reagent to corresponding miniflow channel to the liquid road system is configured to independently to two at least miniflow channel circulation reagents of sample detection device, promptly, realizes the independent control to two at least miniflow channels of sample detection device through a liquid road system.
The utility model discloses a liquid road system provides reagent and washing reagent to sample detection device through the runner on the little flow liquid way board, compares with the liquid road system that adopts traditional pipeline to carry out liquid drive and selection, has reduced the runner volume, has practiced thrift the reagent quantity.
The utility model discloses a miniflow liquid circuit board among the liquid way system can make through injection molding process, possesses certain cost advantage when mass production.
The utility model discloses a sample detection device includes the miniflow channel device, and the miniflow channel device is formed with two at least miniflow channels, places two at least samples in two at least miniflow channels, and two at least samples are the sample of same type or the sample of different grade type to can detect two at least or two kinds of samples simultaneously, also can successively detect two at least or two kinds of samples.
Drawings
Fig. 1 is a block diagram of a sample detection system in accordance with one embodiment of the present invention.
Fig. 2A is a schematic view of a sample testing device according to an embodiment of the present invention.
Fig. 2B isbase:Sub>A schematic longitudinal cross-sectional view of the sample testing device according to an embodiment of the present invention, taken along the dashed linebase:Sub>A-base:Sub>A' in fig. 2A.
Fig. 2C is a schematic transverse cross-sectional view of the sample testing device according to an embodiment of the present invention, taken along the dashed line B-B' in fig. 2A.
Fig. 2D is a top view of a sample testing device according to one embodiment of the present invention.
Fig. 2E is a top view of a sample testing device according to another embodiment of the present invention.
Fig. 3 is a schematic diagram of the overall structure of the fluid path system according to an embodiment of the present invention.
Fig. 4 is a schematic view of a microfluidic circuit board according to an embodiment of the present invention.
Fig. 5 is a schematic diagram of the connection of holes on a microfluidic circuit board to other components, according to an embodiment of the present invention.
Figure 6 is a schematic view of a sample detection system according to one embodiment of the present invention.
Detailed Description
Embodiments of the present invention will be described below with reference to the drawings.
Reference will now be made in detail to the exemplary embodiments, examples of which are illustrated in the accompanying drawings. The following description refers to the accompanying drawings in which the same numbers in different drawings represent the same or similar elements unless otherwise indicated. The embodiments described in the following exemplary embodiments do not represent all embodiments consistent with the present application. Rather, they are merely examples of apparatus and methods consistent with certain aspects of the present application, as detailed in the appended claims.
The terminology used in the present application is for the purpose of describing particular embodiments only and is not intended to be limiting of the application. As used in this application and the appended claims, the singular forms "a", "an", and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise. It should also be understood that the term "and/or" as used herein refers to and encompasses any and all possible combinations of one or more of the associated listed items. The word "comprising" or "comprises", and the like, means that the element or item listed after "comprises" or "comprising" is inclusive of the element or item listed after "comprising" or "comprises", and the equivalent thereof, and does not exclude additional elements or items.
Fig. 1 is a block diagram of a sample detection system in accordance with one embodiment of the present invention.
The utility model discloses a sample detection system includes liquid way system 200 and sample detection device 100.
The fluid path system 200 is configured to independently circulate reagents to at least two microchannels of the sample detection device 100. The fluid path system 200 includes a microfluidic circuit board 201, a reagent cartridge 202, a sample introduction device 203, a reagent selection valve 204, a flow channel selection valve 205, and a drive pump 206.
The microfluidic circuit board 201 is connected to the sample detection device 100 via the channel selection valve 205, and the microfluidic circuit board 201 includes at least two input channels corresponding to the at least two microchannels, each input channel being configured to deliver a reagent to the corresponding microchannel.
The cartridge 202 is connected to the microfluidic circuit board 201 via a sample introduction device 203 and a drive pump 206, and the cartridge 202 is configured to store reagents.
A sample introduction device 203 is coupled to the microfluidic circuit board 201, the sample introduction device 203 being configured to be able to enter the cartridge 201 to contact one or more reagents, the one or more reagents being transported to the microfluidic circuit board 201 via the sample introduction device 203.
A reagent selection valve 204 is coupled to the microfluidic circuit board 201, the reagent selection valve 204 being configured to select a particular reagent from the one or more reagents delivered at a particular time.
A flow channel selector valve 205 is coupled to the microfluidic circuit board 201, the flow channel selector valve 205 being configured to deliver a selected reagent to one or more of the at least two input flow channels.
A drive pump 206 is coupled to microfluidic circuit board 201, and drive pump 206 is configured to provide a driving force for the flow of reagents within sample testing device 100 and microfluidic circuit board 200.
Fig. 2A is a schematic view of a sample testing device according to an embodiment of the present invention. Fig. 2B isbase:Sub>A schematic longitudinal cross-sectional view of the sample testing device according to an embodiment of the present invention, taken along the dashed linebase:Sub>A-base:Sub>A' in fig. 2A. Fig. 2C is a schematic cross-sectional view of the sample detection device according to an embodiment of the present invention, taken along the dashed line B-B' in fig. 2A.
The sample testing device 100 is configured to detect biochemical reactions of at least two samples in at least two microchannels with a selected reagent. The sample detection apparatus 100 includes an optical signal detection apparatus 101 and a micro flow channel apparatus 102.
The optical signal detection device 101 includes a plurality of image sensor chip units 1011. For example, as shown in fig. 2A, the optical signal detection apparatus 101 includes ten image sensor chip units 1011, wherein every five image sensor chip units 1011 are connected inbase:Sub>A row in the direction of the dotted linebase:Sub>A-base:Sub>A', so that two rows of image sensor chip units 1011 are formed on the optical signal detection apparatus 101. The image sensor chip unit 1011 takes the form of a CMOS image sensor chip unit or the like.
The micro channel device 102 is disposed on the upper surface of the optical signal detection device 101, and at least two micro channels 1021 are formed in the micro channel device 102, and each micro channel 1021 corresponds to at least one image sensor chip unit 1011. For example, as shown in FIG. 2A, the micro flow channel device 102 is formed with two micro flow channels 1021 extending alongbase:Sub>A broken line A-A', the two micro flow channels 1021 being independent of each other, each micro flow channel 1021 covering and corresponding tobase:Sub>A row of five image sensor chip units 1011.
Specifically, the micro flow channel device 102 includes a housing 1022, at least two reagent inflow ports 1023 and at least two reagent outflow ports 1024 are formed on the housing 1022, at least two micro flow channels 1021 are formed between the lower surface of the housing 1022 and the upper surface of the optical signal detection device 101, and each micro flow channel 1021 corresponds to one reagent inflow port 1023 and one reagent outflow port 1024. For example, as shown in fig. 2B and 2C, the housing 1022 is closely attached to the upper surface of the optical signal detection device 101 at the position of the side wall 1025, and the side wall 1025 is continuous so as to enclose a cavity, which is partitioned into two micro flow channels 1021. One through hole is provided in each of the housings 1022 at both ends in the longitudinal direction of each micro flow channel 1021 as a corresponding reagent inlet 1023 and a corresponding reagent outlet 1024.
Each micro flow channel 1021 and the corresponding reagent flow inlet 1023 and reagent flow outlet 1024 are configured such that the selected reagent flows into the corresponding micro flow channel 1021 via the corresponding reagent flow inlet 1023, reaches the surface of the corresponding image sensor chip unit 1011, and flows out from the corresponding reagent flow outlet 1024. For example, as shown in fig. 2A to 2C, the width of each micro flow channel 1021 is the same as or slightly larger than the width of the image sensor chip units 1011, and the length of each micro flow channel 1021 is about the length of five image sensor chip units 1011 or slightly larger, so that each micro flow channel 1021 can cover its corresponding five image sensor chip units 1011. Both ends of each micro flow channel 1021 in the longitudinal direction are configured in a shape that gradually narrows, and although this makes part of the surface of the image sensor chip unit 1011 at both ends of the micro flow channel 1021 covered with the side wall 1025 and thus cannot be fully utilized, it is advantageous that the reagent that has entered the micro flow channel 1021 from the reagent inflow port 1023 can reach all the surfaces of the five image sensor chip units 1011 covered with the micro flow channel 1021 and can flow out entirely from the reagent outflow port 1024 without remaining in the micro flow channel 1021. Of course, the micro flow channel 1021 may be of other suitable shapes to achieve the same effect.
The upper surface of the image sensor chip unit 1011 is usually subjected to a certain biological or chemical treatment so as to adsorb a sample placed on the surface and a reagent flowing through the surface, the reagent reacts with the sample to generate a corresponding optical signal, and the optical signal is incident on the image sensor chip unit 1011 and is read and analyzed by the image sensor chip unit 1011 to detect the sample.
Fig. 2D is a top view of a sample testing device according to one embodiment of the present invention. Fig. 2E is a top view of a sample testing device according to another embodiment of the present invention.
As shown in fig. 2D, the micro flow channel device 102 is formed with two independent micro flow channels 1021, and each micro flow channel 1021 covers five image sensor chip units 1011. As shown in fig. 2E, the micro flow channel device 102 is formed with five independent micro flow channels 1021, and each micro flow channel 1021 covers two image sensor chip units 1011.
In the above embodiment, the micro flow channel device 102 includes 2 or 5 micro flow channels 1021, and each micro flow channel 1021 covers the same number of image sensor chip units 1011, but according to different needs, the micro flow channel device 102 of the present invention may be configured to include other numbers of micro flow channels 1021, for example, 10, and each micro flow channel 1021 may be configured to cover different numbers of image sensor chip units 1011, for example, 1 to 100.
Fig. 3 is a schematic diagram of the overall structure of the fluid path system according to an embodiment of the present invention.
The reagent cartridge 202 includes a reaction and washing reagent zone 207, and the reaction and washing reagent zone 207 is configured to store reaction reagents and washing reagents required in a sample detection process. The reaction and cleaning reagent region 207 is sealed by a sealing film (e.g., an aluminum film) to ensure that the reaction and cleaning reagents are not contaminated before use, and the sample introduction device 203 may pierce the sealing film and come into contact with the reaction and cleaning reagents.
The kit 202 includes a waste recovery area 208, and the waste recovery area 208 is configured to recover reacted reagents at the end of the sample detection.
Fig. 4 is a schematic view of a microfluidic circuit board according to an embodiment of the present invention.
The microfluidic circuit board 201 comprises sample injection device hole sites 301-318, reagent flow channels 401-418, a reagent selection valve hole site 501, a reagent selection flow channel 601, a flow channel selection valve hole site 701, input flow channels 801-802 and output flow channels 901-902.
The sample introduction device 203 is connected with the sample introduction device well sites 301-318, the sample introduction device 203 comprises liquid pipelines, and the reaction reagents and/or the cleaning reagents are conveyed from the reaction and cleaning reagent zone 207 of the reagent kit 202 to the reagent flow channels 401-418 via the liquid pipelines of the sample introduction device 203.
The reagent selection valve 204 is connected to the reagent selection valve aperture 501 and the reagent selection valve 204 is configured to open or close one or more of the reagent flow channels 401-418 so that a particular reagent is selected from the reagent flow channels 401-418 at a particular time for delivery to the reagent selection flow channel 601.
The flow channel selection valve 205 is connected to the flow channel selection valve hole 701, and the flow channel selection valve 205 is configured to open or close one or more of the input flow channels 801 to 802, so as to deliver the selected reagent to one or more of the input flow channels 801 to 802, and deliver the selected reagent to the corresponding micro flow channel of the sample detection apparatus 100, so that the selected reagent and the sample in the corresponding micro flow channel perform a biochemical reaction.
The drive pump 206 is connected to the output channels 901 to 902, and the output channels 901 to 902 are configured to deliver the reacted reagent from the micro flow channel to the waste liquid recovery region 208 of the cartridge 202.
Fig. 5 is a schematic diagram of the connection of holes on a microfluidic circuit board to other components, according to an embodiment of the present invention.
The sample injection device 203 and the microfluidic circuit board 201 are hermetically connected through the sample injection device hole sites 301-318. The reagent selection valve 204 is in sealed connection with the microfluidic circuit board 201 through the reagent selection valve hole site 501. The flow channel selection valve 205 and the microfluidic circuit board 201 are in sealed connection through a flow channel selection valve hole 701.
Figure 6 is a schematic view of a sample detection system according to one embodiment of the present invention.
The reaction and wash reagents are pre-stored in the reaction and wash reagent zone 207 of the kit 202. The reactant and wash reagents enter the reagent selection valve 204 via reagent flow channels 401-418 under the driving force provided by the drive pump 206. The reagent selection valve 204 selects a specific reagent to enter the flow channel selection valve 205 through the reagent selection flow channel 601 according to a predetermined timing. The flow channel selection valve 205 allows only the selected reagent to enter one of the input flow channels 801 to 802 or allows the selected reagent to enter a plurality of the input flow channels 801 to 802 according to a predetermined timing. The selected reagent is delivered to the corresponding micro flow channel of the sample detection device 100, so that the selected reagent and the sample in the corresponding micro flow channel are subjected to biochemical reaction. After the reaction of the reagent in the sample detection device 100 is completed, the reagent is recovered to the waste liquid recovery area 208 of the reagent cartridge 202 through the corresponding output flow channels 901-902.
The fluid circuit system 200 also includes a pressure detection device configured to detect a pressure in the fluid circuit system.
A method of operating a sample detection system in accordance with an embodiment of the present invention is described below.
And 2, enabling the sample introduction device to enter the kit to be contacted with one or more reagents.
And 3, driving one or more reagents into the microfluidic circuit board by a driving pump.
Step 4, selecting a specific reagent from the one or more reagents at a specific time by the reagent selection valve.
And 5, conveying the selected reagent to an input flow channel of the microfluidic circuit board by the flow channel selection valve, conveying the selected reagent to a corresponding micro flow channel of the micro flow channel device, and enabling the selected reagent to perform biochemical reaction with a sample in the corresponding micro flow channel.
And 6, detecting the generated biochemical reaction by using an optical signal detection device.
And 7, repeating the steps 2-6 to enable the reagent to carry out biochemical reaction with the sample in the corresponding micro flow channel.
At least two samples in at least two micro flow channels of the micro flow channel device can be the same type of sample or different types of samples.
It will be appreciated that steps 4-6 may be repeated at different times for samples in a particular microchannel according to a predetermined timing sequence.
By the operation method, the liquid path system enables the sample detection device to detect at least two or two types of samples in sequence.
Another method of operating a sample detection system according to one embodiment of the present invention is described below.
And 2, enabling the sample introduction device to enter the kit to be contacted with one or more reagents.
And 3, driving one or more reagents into the microfluidic circuit board by a driving pump.
Step 4, selecting a specific reagent from the one or more reagents at a specific time by the reagent selection valve.
And 5, conveying the selected reagent to at least two input channels of the microfluidic circuit board by the channel selection valve, conveying the selected reagent to at least two corresponding microchannels of the microchannel device, and enabling the selected reagent to simultaneously perform biochemical reaction with at least two or two types of samples in the at least two corresponding microchannels.
And 6, detecting the generated biochemical reaction by using an optical signal detection device.
And 7, repeating the steps 2-6 to enable the reagent to carry out biochemical reaction with the sample in the corresponding micro flow channel.
At least two samples in at least two micro flow channels of the micro flow channel device may be the same type of sample or different types of samples.
It will be appreciated that steps 4-6 may be repeated at different times for samples in a particular microchannel according to a predetermined timing sequence.
By the operation method, the liquid path system enables the sample detection device to simultaneously detect at least two or two types of samples.
While the invention has been shown and described with reference to certain preferred embodiments, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention.
Claims (10)
1. A sample detection system, comprising a fluid path system and a sample detection device;
the fluid path system configured to independently circulate reagents to at least two microchannels of the sample detection device, the fluid path system comprising:
the microfluidic circuit board is connected with the sample detection device and comprises at least two input channels corresponding to the at least two microchannels, and each input channel is configured to convey a reagent to the corresponding microchannel;
a cartridge coupled to the microfluidic circuit board, the cartridge configured to store a reagent;
a sample introduction device coupled to the microfluidic circuit board, the sample introduction device configured to be able to enter the cartridge to contact one or more reagents, the one or more reagents being delivered to the microfluidic circuit board via the sample introduction device;
a reagent selection valve coupled to the microfluidic circuit board, the reagent selection valve configured to select a particular reagent from the one or more reagents delivered at a particular time;
a flow channel selector valve coupled to the microfluidic circuit board, the flow channel selector valve configured to deliver a selected reagent to one or more of the at least two input flow channels;
a drive pump coupled to the microfluidic circuit board, the drive pump configured to provide a driving force for the flow of reagents within the sample detection device and the microfluidic circuit board; and is
The sample detection device is configured to detect biochemical reactions of at least two samples in the at least two micro flow channels with a selected reagent, and comprises:
an optical signal detection device including a plurality of image sensor chip units;
and the micro-channel device is arranged on the upper surface of the optical signal detection device and is provided with at least two micro-channels, and each micro-channel corresponds to at least one image sensor chip unit.
2. The sample detection system of claim 1, wherein the reagent cartridge comprises a reaction and wash reagent zone configured to store a reaction reagent and a wash reagent.
3. The sample detection system of claim 1, wherein the cartridge comprises a waste recovery zone, and the microfluidic circuit board comprises at least two output channels corresponding to the at least two microchannels, each output channel being configured to deliver reacted reagent from the corresponding microchannel to the waste recovery zone.
4. The sample detection system of claim 2, wherein the sample introduction device is coupled to a sample introduction device well site of the microfluidic circuit board, the microfluidic circuit board comprising one or more reagent flow channels, one or more reaction and/or wash reagents being transported from the reaction and wash reagent zones via the sample introduction device to the one or more reagent flow channels.
5. The sample detection system of claim 4, wherein the sample introduction device comprises a liquid line.
6. The sample detection system of claim 4, wherein the reagent selection valve is coupled to a reagent selection valve aperture of the microfluidic circuit board, the microfluidic circuit board including a reagent selection flow channel, the reagent selection valve configured to select a particular reagent from the one or more reagent flow channels to be delivered to the reagent selection flow channel at a particular time.
7. The sample detection system of claim 1, wherein the flow channel selection valve is coupled to a flow channel selection valve aperture of the microfluidic circuit board.
8. The sample detection system of claim 3, wherein the drive pump is coupled to the at least two output flow channels of the microfluidic circuit board.
9. The sample detection system of claim 1, wherein the fluid path system further comprises a pressure detection device configured to detect a pressure in the fluid path system.
10. The sample detection system according to claim 1, wherein the microchannel device comprises a housing, the housing having at least two reagent inlet ports and at least two reagent outlet ports, the at least two microchannels being formed between a lower surface of the housing and an upper surface of the optical signal detection device, each microchannel corresponding to one reagent inlet port and one reagent outlet port;
each micro flow channel and the corresponding reagent flow-in port and reagent flow-out port are configured such that the selected reagent flows into the corresponding micro flow channel via the corresponding reagent flow-in port, reaches the surface of the corresponding image sensor chip unit, and flows out from the corresponding reagent flow-out port.
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| CN202220887773.6U CN217542822U (en) | 2022-04-18 | 2022-04-18 | Sample detection system |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116144480A (en) * | 2023-04-20 | 2023-05-23 | 上海芯像生物科技有限公司 | Fluid system, temperature control method thereof and fluid state monitoring method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116144480A (en) * | 2023-04-20 | 2023-05-23 | 上海芯像生物科技有限公司 | Fluid system, temperature control method thereof and fluid state monitoring method |
| CN116144480B (en) * | 2023-04-20 | 2023-07-28 | 上海芯像生物科技有限公司 | Fluid system, temperature control method thereof and fluid state monitoring method |
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