CN114668889B - Regeneration and repair dressing containing mussel-like mucin and preparation method thereof - Google Patents
Regeneration and repair dressing containing mussel-like mucin and preparation method thereof Download PDFInfo
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- CN114668889B CN114668889B CN202210322960.4A CN202210322960A CN114668889B CN 114668889 B CN114668889 B CN 114668889B CN 202210322960 A CN202210322960 A CN 202210322960A CN 114668889 B CN114668889 B CN 114668889B
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- parts
- mucin
- mussel
- regeneration
- repair dressing
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- 235000010841 Silybum marianum Nutrition 0.000 claims abstract description 33
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
- A61L26/008—Hydrogels or hydrocolloids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
- A61L26/0023—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
- A61L26/0028—Polypeptides; Proteins; Degradation products thereof
- A61L26/0047—Specific proteins or polypeptides not covered by groups A61L26/0033 - A61L26/0042
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0057—Ingredients of undetermined constitution or reaction products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
- A61L26/0066—Medicaments; Biocides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/30—Compounds of undetermined constitution extracted from natural sources, e.g. Aloe Vera
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Materials Engineering (AREA)
- Engineering & Computer Science (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Dispersion Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Medicinal Preparation (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The invention provides a regeneration and repair dressing containing mussel-like mucin and a preparation method thereof, wherein the regeneration and repair dressing comprises the following raw materials: mussel-like mucin, a toughening gel material, jojoba oil, silybum marianum seed oil, litsea cubeba extract, rhamnolipid, L-carnitine, an amino acid composition, hyaluronic acid, menthane formyl ethylamine, glycerol and water. The regeneration repair dressing containing the mussel-like mucin, which is obtained by the invention, can effectively prevent microbial infection in the recovery process, can achieve the effect of fully protecting the new tissues, further promote the rapid healing of the wound surface, simultaneously avoid physical injury, maintain the wettability of the wound surface, provide the optimal recovery environment for the wound surface healing, and reduce the pain of patients.
Description
Technical Field
The invention relates to the technical field of medical dressings, in particular to a regeneration and repair dressing containing mussel-like mucin and a preparation method thereof.
Background
The wound surface needs to be healed through four stages of a coagulation stage, an inflammation stage, a proliferation stage and a remodeling stage, and effective healing is finally realized. The healing result is not ideal, and usually stagnates in the inflammatory or proliferative phase, which causes persistent inflammatory reaction, and finally the wound surface is not healed for a long time. The existing medical dressing has various structures and functions, and although the medical dressing has breakthrough progress, a good protective barrier environment cannot be formed for the repair and healing of a wound surface so as to maintain the transportation of proper blood and nutrients of the wound surface, promote cell activity and skin repair and regeneration capacity, further avoid persistent inflammation caused by stagnation in an inflammation period, reduce the immunity of an organism, prolong the time of wound surface healing and cause the formation of a chronic wound surface.
The proper dressing can avoid the pollution of bacteria or other substances to the wound surface, simultaneously avoid physical injury, maintain the wettability of the wound surface, provide an optimal environment for the wound surface healing to enhance the function recovery of the wound surface, and most importantly, reduce the occurrence of infection and inflammatory reaction so as to improve the wound surface healing speed and reduce the pain of patients.
Disclosure of Invention
In view of the above, the present invention aims to provide a regeneration repair dressing containing mussel-like mucin and a preparation method thereof, wherein the regeneration repair dressing containing mussel-like mucin acts on a skin wound surface, can effectively prevent microbial infection in a recovery process, can fully protect a new tissue, further promote rapid healing of the wound surface, simultaneously avoid physical damage, maintain wettability of the wound surface, provide an optimal recovery environment for wound surface healing, and reduce pain of a patient.
The technical scheme of the invention is realized as follows:
a regeneration and repair dressing containing mussel-like mucin comprises the following raw materials in parts by weight: 12-32 parts of mussel-like mucin, 4-8 parts of toughened gel material, 1-5 parts of jojoba oil, 2-8 parts of silybum marianum seed oil, 4-10 parts of litsea cubeba extract, 1-3 parts of rhamnolipid, 0.5-2 parts of L-carnitine, 0.6-1.8 parts of amino acid composition, 6-10 parts of hyaluronic acid, 0.8-1.5 parts of menthane formylethylamine, 10-20 parts of glycerol and 100-300 parts of water.
Preferably, the feed comprises the following raw materials in parts by weight: 22 parts of mussel-like mucin, 6 parts of toughened gel material, 3 parts of jojoba oil, 4 parts of silybum marianum seed oil, 7 parts of litsea cubeba extract, 2 parts of rhamnolipid, 1.2 parts of L-carnitine, 1.2 parts of amino acid composition, 8 parts of hyaluronic acid, 1.1 parts of menthane formylethylamine, 15 parts of glycerol and 200 parts of water.
The rhamnolipid and the glycerol are selected, so that the active ingredients of mussel-like mucin, jojoba oil, silybum marianum seed oil and litsea cubeba extract can be favorably fused, the speed of the skin permeation from a surface layer is promoted, the nutrition supply of the skin is improved, the skin is nourished and lubricated, the L-carnitine, the amino acid composition and the hyaluronic acid can improve the activity of a skin repair factor, the skin is relieved and repaired, the amino acid is a biological substance required by a human body, the immunity is favorably improved, and the healing of a wound surface is promoted; the components are mutually cooperated, so that the wound dressing has good cell compatibility and affinity, and effectively reduces wound infection and inflammatory reaction.
Further, the amino acid composition consists of valine, lysine, serine, glutamic acid, proline, arginine, aspartic acid and glycine; the mass ratio of valine, lysine, serine, glutamic acid, proline, arginine, aspartic acid and glycine is 0.8-1:0.2-0.3:0.2-0.3:0.5-0.55:0.3-0.4:0.3-0.4:0.6-0.8:0.6-0.8.
Further, the toughening gel material is prepared from the following components in a mass ratio of 2:9-13 beta-vegetable oil and poloxamer.
Furthermore, the content of the protein of the mussel-like mucin is 45-60%, and the molecular weight is 1.2-2.5KDa.
Furthermore, the mussel-like mucin is a fermentation extract, and is obtained by fermenting perna viridis with bacillus natto and rhodotorula benthica, leaching with hot water, and precipitating with isopropanol.
Further, the extraction method of the silybum marianum seed oil comprises the following steps: oven drying herba Silybi Mariani, pulverizing, adding complex enzyme, performing ultrasonic enzymolysis, extracting with 35-45% ethanol solution, collecting oil phase, repeatedly extracting the water phase for 2-3 times, mixing oil phases, and concentrating to obtain Silybum marianum seed oil; the compound enzyme is prepared from the following components in percentage by mass 2:1 and beta-glucanase.
Further, the preparation method of the litsea cubeba extract comprises the following steps: oven drying and pulverizing Litsea cubeba, sieving with 60-80 mesh sieve, soaking in 6-8M hydrochloric acid solution, soaking at 70-80 deg.C for 40-60min, adding cellulase, performing enzymolysis at 30-40 deg.C for 1-3 hr, adding xylanase, performing microwave enzymolysis at 350-450W for 20-40min, centrifuging, collecting supernatant, and concentrating to obtain Litsea cubeba extract.
The preparation method of the mussel-like mucin comprises the following steps:
step S1: adding toughening gel material and water into mussel-like mucin, and ultrasonically dispersing at 45-55 deg.C and 400-600W for 40-60min to obtain pre-dispersion solution;
step S2: mixing jojoba oil, silybum marianum seed oil, fructus Litseae extract, rhamnolipid and glycerol, and stirring at 30-40 deg.C to obtain oil phase solution;
and step S3: mixing L-carnitine, the amino acid composition and hyaluronic acid to obtain an aqueous phase solution;
and step S4: mixing the oil phase solution and the water phase solution, homogenizing at 6000-10000r/min for 6-10min, adding the pre-dispersion solution, stirring at 200-400r/min for 30-50min, performing secondary ultrasonic dispersion at 45-55 deg.C and 200-400W for 20-30min, adding menthane formylethylamine, and mixing to obtain regeneration repair dressing containing mussel-like mucin; the invention firstly carries out ultrasonic dispersion on the mussel-like mucin and the toughened gel material to obtain a pre-dispersion solution, then respectively prepares an oil phase solution and a water phase solution according to the characteristics of raw materials, and finally carries out homogenization and secondary ultrasonic dispersion to enhance the dispersion stability of a mixed system, improve the molecular mechanical stability of the system when the mixing uniformity of the components is improved, and further improve the moisture retention performance.
Compared with the prior art, the invention has the beneficial effects that:
the invention selects mussel-like mucin, toughening gel material, jojoba oil, silybum marianum seed oil, litsea cubeba extract, rhamnolipid, L-carnitine, amino acid composition, hyaluronic acid, menthane formyl ethylamine and other components, and the like, and the regenerative repair dressing containing the mussel-like mucin is prepared according to the optimal proportion, so that the proliferative activity of fibroblasts can be enhanced, the cell activity can be improved, the regenerative capability of the skin at the injured part can be promoted, the wound healing degree can be improved, and the optimal environment is provided for the wound healing so as to enhance the functional recovery; meanwhile, the components are mutually cooperated, so that the obtained regeneration repair dressing containing the mussel-like mucin has high biological activity, no toxicity and good performance, can effectively prevent microbial infection in the recovery process, can fully protect the new tissue, and further promotes the wound to be rapidly healed.
The mussel mucin serving as a core active ingredient belongs to a non-animal source material, is extracted by a biological fermentation technology, and has high safety. The regeneration repair dressing containing the mussel-like mucin can be stably and effectively stored for a long time by a scientific formula and a unique process, and has a better effect by synergistic action with other raw materials when in use, and the timely repair and regeneration healing capabilities of skin wound surfaces are promoted.
In addition, according to the invention, the perna viridis is fermented by adopting bacillus natto and rhodotorula benthamii, the fermented product is extracted by hot water, then isopropanol is precipitated and extracted to obtain mussel-like mucin with high protein content and high molecular weight, and silybum marianum seed oil is extracted by ultrasonic enzymolysis and low-concentration ethanol, and the litsea cubeba extract is obtained by high-acid high-heat soaking and sequential enzymolysis, so that the bioactive components of the mussel-like mucin, the silybum marianum seed oil and the litsea cubeba extract are high, and the components can be organically combined, thereby being more beneficial to improving the activity of cells, promoting the proliferation activity of the cells and maintaining and regulating the growth stable period of the cells; the invention also selects the combination of the beta-vegetable oil and the poloxamer as the toughening gel material, so that the dressing has good cell affinity and tissue adhesion, and the stability of the molecular mechanics can be enhanced.
Detailed Description
In order to better understand the technical content of the invention, specific examples are provided below to further illustrate the invention.
The experimental methods used in the examples of the present invention are all conventional methods unless otherwise specified.
The materials, reagents and the like used in the examples of the present invention can be obtained commercially without specific description.
Example 1
A regeneration and repair dressing containing mussel-like mucin comprises the following raw materials in parts by weight: 22 parts of mussel-like mucin, 6 parts of toughened gel material, 3 parts of jojoba oil, 4 parts of silybum marianum seed oil, 7 parts of litsea cubeba extract, 2 parts of rhamnolipid, 1.2 parts of L-carnitine, 1.2 parts of amino acid composition, 8 parts of hyaluronic acid, 1.1 parts of menthane formylethylamine, 15 parts of glycerol and 200 parts of water.
The amino acid composition consists of valine, lysine, serine, glutamic acid, proline, arginine, aspartic acid and glycine; the mass ratio of valine, lysine, serine, glutamic acid, proline, arginine, aspartic acid and glycine is 0.9:0.2:0.2:0.5:0.3:0.3:0.6:0.6.
the toughening gel material is prepared from the following components in percentage by mass 2:11 and poloxamer.
The mussel-like mucin is a fermentation extract, and the extraction method comprises the following steps: perna viridis is fermented by bacillus natto and rhodotorula benthica, the fermented product is extracted by hot water, isopropanol is precipitated and extracted to obtain mussel-like mucin, and the content of the mussel-like mucin is 51.8 percent and the molecular weight is 1.86KDa by determination.
The extraction method of the silybum marianum seed oil comprises the following steps: drying and crushing the silybum marianum, and adding the materials in a mass ratio of 2:1 carrying out ultrasonic enzymolysis on the compound enzyme of pectinase and beta-glucanase, extracting by adopting an ethanol solution with the mass concentration of 40%, collecting an oil phase, repeatedly extracting the water phase for 3 times, combining the oil phases, and concentrating to obtain the silybum marianum seed oil.
The preparation method of the litsea cubeba extract comprises the following steps: oven drying and pulverizing fructus Litseae, sieving with 70 mesh sieve, soaking in 7M hydrochloric acid solution, soaking at 75 deg.C for 50min, adding cellulase, performing enzymolysis at 35 deg.C for 2h, adding xylanase, performing microwave enzymolysis at 400W for 30min, centrifuging, collecting supernatant, and concentrating to obtain fructus Litseae extract.
The regeneration and repair dressing containing the mussel-like mucin comprises the following steps:
step S1: adding toughening gel material and water into mussel-like mucin, and ultrasonically dispersing at 50 deg.C and 500W for 50min to obtain pre-dispersion solution;
step S2: mixing jojoba oil, silybum marianum seed oil, litsea cubeba extract, rhamnolipid and glycerol, and stirring and dissolving at 35 ℃ to obtain an oil phase solution;
and step S3: mixing L-carnitine, the amino acid composition and hyaluronic acid to obtain an aqueous phase solution;
and step S4: mixing the oil phase solution and the water phase solution, homogenizing at 8000r/min for 8min, adding the pre-dispersion solution, stirring at 300r/min for 40min, ultrasonically dispersing at 50 deg.C and 300W for 25min, adding menthane formylethylamine, and mixing to obtain the regeneration repair dressing containing mussel-like mucin.
Example 2
A regeneration and repair dressing containing mussel-like mucin comprises the following raw materials in parts by weight: 12 parts of mussel-like mucin, 4 parts of toughened gel material, 1 part of jojoba oil, 2 parts of silybum marianum seed oil, 4 parts of litsea cubeba extract, 1 part of rhamnolipid, 0.5 part of L-carnitine, 0.6 part of amino acid composition, 6 parts of hyaluronic acid, 0.8 part of menthane formyl ethylamine, 10 parts of glycerol and 100 parts of water.
The raw materials and the preparation method of the regeneration and repair dressing containing the mussel-like mucin are the same as the example 1.
Example 3
A regeneration and repair dressing containing mussel-like mucin comprises the following raw materials in parts by weight: 32 parts of mussel-like mucin, 8 parts of toughened gel material, 5 parts of jojoba oil, 8 parts of silybum marianum seed oil, 10 parts of litsea cubeba extract, 3 parts of rhamnolipid, 2 parts of L-carnitine, 1.8 parts of amino acid composition, 10 parts of hyaluronic acid, 1.5 parts of menthane formyl ethylamine, 20 parts of glycerol and 300 parts of water.
The raw materials and the preparation method of the regeneration and repair dressing containing the mussel-like mucin are the same as example 1.
Example 4
The amino acid composition consists of valine, lysine, serine, glutamic acid, proline, arginine, aspartic acid and glycine; the mass ratio of valine to lysine to serine to glutamic acid to proline to arginine to aspartic acid to glycine is 1:0.3:0.3:0.55:0.4:0.4:0.8:0.8.
the toughening gel material is prepared from the following components in percentage by mass 2:13 beta-vegetable oil and poloxamer.
The raw material ratio and the preparation method of the regeneration and repair dressing containing the mussel-like mucin are the same as the example 1.
Example 5
The extraction method of the mussel-like mucin comprises the following steps: perna viridis is fermented by bacillus natto and bacillus subtilis, the fermented product is extracted by hot water, isopropanol is precipitated and extracted to obtain mussel-like mucin, and the content of the mussel-like mucin is 32.8 percent and the molecular weight is 0.8KDa by determination.
The raw material ratio and the preparation method of the regeneration and repair dressing containing the mussel-like mucin are the same as the example 1.
Example 6
The extraction method of the mussel-like mucin comprises the following steps: perna viridis is fermented by bacillus natto and rhodotorula benthica, the fermented product is extracted by hot water, and then is subjected to ethanol precipitation extraction to obtain mussel-like mucin, and the content of the mussel-like mucin is measured to be 43.7%, and the molecular weight is 1.2KDa.
The raw material ratio and the preparation method of the regeneration and repair dressing containing the mussel-like mucin are the same as the example 1.
Example 7
The extraction method of the silybum marianum seed oil comprises the following steps: oven drying herba Silybi Mariani, pulverizing, adding pectinase, performing ultrasonic enzymolysis, extracting with 85% ethanol solution, collecting oil phase, extracting the water phase for 3 times, mixing the oil phases, and concentrating to obtain Silybum marianum seed oil.
The preparation method of the litsea cubeba extract comprises the following steps: oven drying and pulverizing Litsea cubeba, sieving with 7 mesh sieve, adding water, soaking at 75 deg.C for 50min, adding cellulase and xylanase, performing microwave enzymolysis at 400W for 2 hr, centrifuging, collecting supernatant, and concentrating to obtain Litsea cubeba extract.
The raw material ratio and the preparation method of the regeneration and repair dressing containing the mussel-like mucin are the same as the example 1.
Comparative example 1
A regeneration and repair dressing containing mussel-like mucin comprises the following raw materials in parts by weight: 45 parts of mussel-like mucin, 6 parts of toughened gel material, 3 parts of jojoba oil, 12 parts of silybum marianum seed oil, 15 parts of litsea cubeba extract, 2 parts of rhamnolipid, 1.2 parts of L-carnitine, 1.2 parts of amino acid composition, 8 parts of hyaluronic acid, 1.1 parts of menthane formylethylamine, 15 parts of glycerol and 200 parts of water.
The raw materials and the preparation method of the regeneration and repair dressing containing the mussel-like mucin are the same as the example 1.
Comparative example 2
A regeneration and repair dressing containing mussel-like mucin adopts commercial mussel mucin to replace the mussel-like mucin, and comprises the following raw materials in parts by weight: 22 parts of mussel mucin, 6 parts of toughened gel material, 3 parts of jojoba oil, 4 parts of silybum marianum seed oil, 7 parts of litsea cubeba extract, 2 parts of rhamnolipid, 1.2 parts of L-carnitine, 1.2 parts of amino acid composition, 8 parts of hyaluronic acid, 1.1 parts of menthane formyl ethylamine, 15 parts of glycerol and 200 parts of water.
The mussel mucin is a commercially available raw material with the purity of 98 percent.
The raw materials and the preparation method of the regeneration and repair dressing containing the mussel-like mucin are the same as example 1.
Comparative example 3
A regeneration and repair dressing containing mussel-like mucin comprises the following raw materials in parts by weight: 22 parts of mussel-like mucin, 6 parts of toughened gel material, 3 parts of jojoba oil, 2 parts of rhamnolipid, 1.2 parts of L-carnitine, 1.2 parts of amino acid composition, 8 parts of hyaluronic acid, 1.1 parts of menthane formyl ethylamine, 15 parts of glycerol and 200 parts of water.
The raw materials and the preparation method of the regeneration and repair dressing containing the mussel-like mucin are the same as example 1.
Comparative example 4
A regeneration and repair dressing containing mussel-like mucin comprises the following raw materials in parts by weight: 22 parts of mussel-like mucin, 6 parts of poloxamer, 3 parts of jojoba oil, 4 parts of silybum marianum seed oil, 7 parts of litsea cubeba extract, 2 parts of rhamnolipid, 1.2 parts of L-carnitine, 1.2 parts of amino acid composition, 8 parts of hyaluronic acid, 1.1 parts of menthane formyl ethylamine, 15 parts of glycerol and 200 parts of water.
The regeneration and repair dressing containing the mussel-like mucin comprises the following steps:
step S1: mixing mussel-like mucin, poloxamer, jojoba oil, silybum marianum seed oil, litsea cubeba extract, rhamnolipid and glycerol, and stirring at 35 deg.C to obtain oil phase solution;
step S2: mixing L-carnitine, an amino acid composition, hyaluronic acid and water to obtain an aqueous phase solution;
and step S3: mixing the oil phase solution and the water phase solution, homogenizing at 8000r/min for 8min, adding menthane formylethylamine, and mixing to obtain the regeneration and repair dressing containing mussel-like mucin.
Test example 1 cell viability test
The MTT colorimetric method is adopted to carry out the test determination of the cell proliferation activity:
cell: mouse fibroblast L929;
cell growth medium: 10% fetal bovine serum DMEM (containing 4000mg/L D-glucose, 2mM L-glutamine, 1.5g/L NaHCO) 3 0.1mM non-essential amino acid, 1.0mM sodium pyruvate), pH 7.4;
the culture conditions are as follows: at 37 ℃ C, 5% CO 2 Culturing under atmosphere and humidity above 90%, subculturing every 3 days, and inoculating cell at density of 1 × 10 5 Per mL;
and (3) testing: the fibroblasts L929 obtained by the culture are blown and beaten uniformly to inoculate the cells with the density of 1 multiplied by 10 4 seed/mL, inoculationCultured in 96-well plates at 37 ℃ with 5% CO 2 Culturing for 12h under the atmosphere and humidity of more than 90 percent, adding 10 mu L of regeneration repair dressing containing mussel-like mucin of 2mg/mL, culturing for 24h, 36h and 48h respectively, adding 10 mu L of MTT solution into each hole, continuously culturing for 4h, discarding culture supernatant, adding 100 mu L of DMSO solution into each hole, oscillating for 10min, measuring and recording photometric value at 570nm wavelength by using a microplate reader, repeating the measurement for three times, taking the added cell growth culture solution as a blank group, taking the regeneration repair dressing not added with mussel-like mucin as a control group, taking the added regeneration repair dressing containing mussel-like mucin as a test group, and taking the cell viability) = (test group-blank group)/(control group-blank group). Times.100, wherein the results are as follows:
the above table shows that the cell viability of the regeneration repair dressing containing the mussel-like mucin obtained by the invention is 103-118% for 24h, 130-149% for 36h and 129-158% for 48h, which indicates that the regeneration repair dressing containing the mussel-like mucin can enhance the proliferation activity of fibroblasts, improve the cell viability, promote the regeneration capability of skin at the injured part, provide an optimal environment for wound healing and accelerate wound healing by selecting the components such as the mussel-like mucin, the toughening gel material, the jojoba oil, the silybum marianum seed oil, the litsea cubeba extract, rhamnolipid, l-carnitine, the amino acid composition, hyaluronic acid, menthane formylethylamine and the like according to a preferable proportion.
Example 1 and examples 5 and 6, compared with comparative example 2, the present invention further finds that perna viridis is fermented by bacillus natto and rhodotorula benthica, then is subjected to hot water extraction, and is subjected to isopropanol alcohol precipitation extraction, so that the obtained mussel-like mucin has high protein content and molecular weight, and meanwhile, the mussel-like mucin with protein content of 45-60% and molecular weight of 1.2-2.5KDa is added, so that the vitality of fibroblasts can be improved.
Compared with the comparative example 3, the silybum marianum seed oil obtained by ultrasonic enzymolysis and low-concentration ethanol extraction and the litsea cubeba extract obtained by high-acid high-heat soaking and sequential enzymolysis are also found in the invention in the example 1 and the example 7, so that the cell activity is favorably improved, and the growth stable period of fibroblasts can be maintained and adjusted.
Compared with the comparative example 4, the example 1 shows that the toughening gel material is formed by adopting the beta-vegetable oil and the poloxamer, so that the prepared regeneration repair dressing containing the mussel-like mucin has good cell affinity and tissue adhesion, the stability of a mechanical structure is enhanced, and the mechanical property of the regeneration repair dressing is prevented from being influenced by cracks or breakage caused by mechanical deformation.
Test example 2 wound healing test
50 healthy white rabbits (with the average weight of 4 kg) are selected, randomly divided into 5 groups, injected and anesthetized, sheared on the back, wiped with 75% alcohol to disinfect the skin, and then a skin biopsy device is adopted to perform artificial wound surface on the back, wherein the wound surface is in a 2cm multiplied by 2cm round shape, and 0.2cm deep tissues including skin, subcutaneous tissues and muscle tissues are cut in the round. Cleaning the wound surface, respectively smearing the regeneration repair dressings containing mussel-like mucin prepared in the examples and the comparative examples on the surface of the wound surface, wrapping the wound surface by using sterile gauze, changing the dressing once every 2 days, disinfecting the wound by using iodophor, measuring the area of the wound surface by using Imagel software, observing the wound surface for 5 days, and calculating the wound surface reduction rate (%) = (S) 0 -S 1 )/S 0 X100, wherein S 0 Area before wound surface treatment, S 1 The area of the wound surface after treatment is measured, C-reactive protein (CRP) is detected from 1d and 3d rabbit auricular venous blood after administration, wound tissues are taken as histopathological sections, and the number of neutral polymorphonuclear leukocytes (PMN) is calculated by using an optical microscope.
As is clear from the above results, the mussel-like mucin-containing food product obtained by the present inventionThe wound surface shrinkage rate of the regeneration and repair dressing is 96.2 percent, PMN is reduced to 1.33 at the 3 rd step, and CRP value is 5.23 mg.L at the 3 rd step -1 The components are mutually cooperated, so that the dressing has excellent anti-infection capacity, inflammatory cells of wound tissues can be quickly reduced, and the contraction and healing speed of the wound are accelerated.
Example 1 compared to comparative examples 1-3, shows that mussel-like mucin, silybum marianum seed oil and litsea cubeba extract have certain healing and anti-inflammatory effects on wound treatment.
Compared with the comparative example 4, the toughened gel material formed by the beta-vegetable oil and the poloxamer has a synergistic effect on wound surface recovery, and can improve the biocompatibility, viscoelasticity and sustainability of the product.
Example 8
The regeneration and repair dressing containing the mussel-like mucin comprises the following steps:
step S1: adding toughening gel material and water into mussel-like mucin, and ultrasonically dispersing at 50 deg.C and 500W for 50min to obtain pre-dispersion solution;
step S2: mixing jojoba oil, silybum marianum seed oil, litsea cubeba extract, rhamnolipid and glycerol, and stirring and dissolving at 35 ℃ to obtain an oil phase solution;
and step S3: mixing L-carnitine, the amino acid composition and hyaluronic acid to obtain an aqueous phase solution;
and step S4: mixing the oil phase solution and the water phase solution, homogenizing at 8000r/min for 6-10min, adding the pre-dispersion solution, stirring at 200-400r/min for 30-50min, adding menthane formylethylamine, and mixing to obtain the regeneration repair dressing containing mussel-like mucin.
The raw material ratio and the preparation method are the same as those of example 1.
Example 9
The regeneration and repair dressing containing the mussel-like mucin comprises the following steps:
step S1: mixing mussel-like mucin, toughening gel material, jojoba oil, silybum marianum seed oil, litsea cubeba extract, rhamnolipid and glycerol, and stirring at 35 deg.C to dissolve to obtain oil phase solution;
step S2: mixing L-carnitine, an amino acid composition, hyaluronic acid and water to obtain an aqueous phase solution;
and step S3: mixing the oil phase solution and the water phase solution, homogenizing at 8000r/min for 8min, adding menthane formylethylamine, and mixing to obtain the regeneration and repair dressing containing mussel-like mucin.
The raw material ratio and the preparation method are the same as those of example 1.
Test example 3 moisture retention test
The regeneration and repair dressings containing mussel-like mucin prepared in examples 1, 8 and 9 were placed in an environment with a temperature of 25 + -2 ℃ and a relative humidity of 55 + -5% for 24 hours, and the moisture retention rate was calculated by a weighing method, and the results are as follows:
| item | Example 1 | Example 8 | Example 9 |
| Moisture retention/degree | 88.6 | 85.9 | 70.0 |
As can be seen from the above table, the moisture retention rates of examples 1 and 8 reach more than 85%, and example 9 is 70%, which is significantly lower than examples 1 and 8, which indicates that the present invention first performs ultrasonic dispersion on the mussel-like mucin and the toughening gel material to obtain a pre-dispersion solution, then prepares an oil phase solution and a water phase solution according to the characteristics of the raw materials, and finally performs homogenization and secondary ultrasonic dispersion to enhance the dispersion stability of the mixed system, and when the mixing uniformity of the components is improved, the molecular mechanical stability of the system is improved, and the moisture retention performance is further improved.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (8)
1. A regeneration and repair dressing containing mussel-like mucin is characterized by comprising the following raw materials in parts by weight: 12-32 parts of mussel-like mucin, 4-8 parts of toughened gel material, 1-5 parts of jojoba oil, 2-8 parts of silybum marianum seed oil, 4-10 parts of litsea cubeba extract, 1-3 parts of rhamnolipid, 0.5-2 parts of L-carnitine, 0.6-1.8 parts of amino acid composition, 6-10 parts of hyaluronic acid, 0.8-1.5 parts of menthane formylethylamine, 10-20 parts of glycerol and 100-300 parts of water; the toughening gel material is prepared from the following components in a mass ratio of 2:9-13 beta-vegetable oil and poloxamer.
2. The regeneration and repair dressing containing the mussel-like mucin according to claim 1, which is characterized by comprising the following raw materials in parts by weight: 22 parts of mussel-like mucin, 6 parts of toughened gel material, 3 parts of jojoba oil, 4 parts of silybum marianum seed oil, 7 parts of litsea cubeba extract, 2 parts of rhamnolipid, 1.2 parts of L-carnitine, 1.2 parts of amino acid composition, 8 parts of hyaluronic acid, 1.1 parts of menthane formylethylamine, 15 parts of glycerol and 200 parts of water.
3. The regenerative repair dressing comprising mussel-like mucin according to claim 1, wherein the amino acid composition is comprised of valine, lysine, serine, glutamic acid, proline, arginine, aspartic acid and glycine; the mass ratio of valine, lysine, serine, glutamic acid, proline, arginine, aspartic acid and glycine is 0.8-1:0.2-0.3:0.2-0.3:0.5-0.55:0.3-0.4:0.3-0.4:0.6-0.8:0.6-0.8.
4. The regeneration and repair dressing containing mussel-like mucin according to claim 1, wherein the mussel-like mucin has a protein content of 45-60% and a molecular weight of 1.2-2.5KDa.
5. The regenerative repair dressing containing mussel-like mucin according to claim 4, wherein the mussel-like mucin is a fermentation extract obtained by fermenting perna viridis with Bacillus natto and Rhodotorula benthica, extracting with hot water, and precipitating with ethanol with isopropanol.
6. The regeneration and repair dressing containing mussel-like mucin according to claim 1, wherein the silybum marianum seed oil is extracted by the following method: oven drying herba Silybi Mariani, pulverizing, adding complex enzyme, performing ultrasonic enzymolysis, extracting with 35-45% ethanol solution, collecting oil phase, repeatedly extracting the water phase for 2-3 times, mixing the oil phases, and concentrating to obtain Silybum marianum seed oil; the composite enzyme is prepared from the following components in percentage by mass 2:1 of pectinase and beta-glucanase.
7. The regeneration and repair dressing containing mussel-like mucin according to claim 1, wherein the litsea cubeba extract is prepared by: oven drying and pulverizing Litsea cubeba, sieving with 60-80 mesh sieve, soaking in 6-8M hydrochloric acid solution, soaking at 70-80 deg.C for 40-60min, adding cellulase, performing enzymolysis at 30-40 deg.C for 1-3 hr, adding xylanase, performing microwave enzymolysis at 350-450W for 20-40min, centrifuging, collecting supernatant, and concentrating to obtain Litsea cubeba extract.
8. The method for preparing a regenerative repair dressing according to any one of claims 1 to 7, comprising the steps of:
step S1: adding toughening gel material and water into mussel-like mucin, and ultrasonically dispersing at 45-55 deg.C and 400-600W for 40-60min to obtain pre-dispersion solution;
step S2: mixing jojoba oil, silybum marianum seed oil, fructus Litseae extract, rhamnolipid and glycerol, and stirring at 30-40 deg.C to obtain oil phase solution;
and step S3: mixing L-carnitine, the amino acid composition and hyaluronic acid to obtain an aqueous phase solution;
and step S4: mixing the oil phase solution and the water phase solution, homogenizing at 6000-10000r/min for 6-10min, adding the pre-dispersion solution, stirring at 200-400r/min for 30-50min, performing secondary ultrasonic dispersion at 45-55 deg.C and 200-400W for 20-30min, adding menthane formylethylamine, and mixing to obtain the regeneration repair dressing containing mussel-like mucin.
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| CN106512066A (en) * | 2016-11-02 | 2017-03-22 | 袁春华 | A preparing method of a mussel protein composite medical dressing |
| CN109395153A (en) * | 2018-10-29 | 2019-03-01 | 王景硕 | A kind of wound repair materials |
| TWI691343B (en) * | 2019-05-02 | 2020-04-21 | 邵明遠 | Use of liquid bandage composition |
| CN111995777B (en) * | 2019-05-31 | 2022-06-03 | 中国科学院青岛生物能源与过程研究所 | Preparation method of PEGDA-mussel adhesive protein-collagen composite hydrogel with strong adhesion and high mechanical strength |
| CN111587945A (en) * | 2020-05-27 | 2020-08-28 | 广州富诺营养科技有限公司 | Sialic acid-containing gel candy and preparation method and application thereof |
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