CN114668680A - Washing-free quick-drying hand disinfectant capable of killing viruses - Google Patents

Washing-free quick-drying hand disinfectant capable of killing viruses Download PDF

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CN114668680A
CN114668680A CN202210249795.4A CN202210249795A CN114668680A CN 114668680 A CN114668680 A CN 114668680A CN 202210249795 A CN202210249795 A CN 202210249795A CN 114668680 A CN114668680 A CN 114668680A
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test
killing
disinfectant
hydrogen peroxide
ethanol
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倪杰
赵立群
姜朴
苏贵斌
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Hubei Microbial Control Biological Technology Co ltd
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Hubei Microbial Control Biological Technology Co ltd
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
    • A61K8/22—Peroxides; Oxygen; Ozone
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • A61P31/14—Antivirals for RNA viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • A61Q17/005—Antimicrobial preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/10—Washing or bathing preparations

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Abstract

The invention discloses a washing-free hand-drying disinfectant capable of killing viruses, belonging to the technical field of disinfection. The paint consists of the following components in percentage by weight: 73-82% of ethanol, 0.1-0.16% of hydrogen peroxide, 0.5-2.0% of glycerol, 0.02-1% of synergistic component and the balance of deionized water, wherein the synergistic component is one or more selected from dehydroacetic acid, sorbic acid, citric acid and lactic acid. Acts for 1 minute according to the requirements of 'disinfection and sanitation Specification', has an average inactivation logarithm value of 4 for poliovirus type 1 vaccine strains, and has good inactivation effect on viruses.

Description

Washing-free quick-drying hand disinfectant capable of killing viruses
Technical Field
The invention relates to the technical field of disinfection, in particular to a washing-free quick-drying hand disinfectant capable of killing viruses.
Background
The hand washing is always an important thing in life, from the previous hand washing before meals to the current people traveling by public transport, the chances of contacting other people or objects are greatly increased, and the chances of disease infection through hands are also increased. The hand washing and sterilizing mode is from the prior soap and perfumed soap to the prior hand washing liquid, the washing effect is better and more, and the use is more and more convenient. Later, the no-clean liquid soap appeared, which does not need to use towels, water sources, soaps and the like, is convenient to carry and use and is gradually accepted by people. The main component of the no-clean quick-drying hand disinfectant capable of killing virus is usually ethanol, such as a classic no-clean quick-drying hand disinfectant capable of killing virus consisting of ethanol, hydrogen peroxide and glycerol.
In the prior art, high-concentration ethanol (such as about 75 percent) can be used for preparing a washing-free quick-drying hand disinfectant capable of killing viruses, and for example, a patent with the application number of CN202010380388.8 discloses a preparation method of a polysaccharide quick-drying hand disinfectant, which comprises the following components in parts by weight: 80ml of ethanol; 0.4ml of hydrogen peroxide; 0.1g of glucan quaternary ammonium salt; 1.5ml of glycerol; 18ml of purified water. In the patent, the quaternary ammonium salt of glucan is used as a disinfectant and a skin care component, and the quaternary ammonium glucan is applied to the hand disinfectant, so that the disinfection performance can be improved, and the effects of preventing bacteria and viruses from diffusing, protecting the skin and the like can be achieved.
Common disinfectant liquid formed by combining ethanol, hydrogen peroxide and glycerol acts for 1 minute according to the requirements of disinfection sanitation standards, and the killing logarithm value of the virus is 2-3, so that the high-efficiency disinfection effect cannot be achieved.
Disclosure of Invention
In order to solve the problems, the embodiment of the invention provides a washing-free quick-drying hand disinfectant capable of killing viruses, which has an average inactivation log value of 4 for poliovirus type 1 vaccine strains and a good virus inactivation effect. The technical scheme is as follows:
the embodiment of the invention provides a washing-free quick-drying hand disinfectant capable of killing viruses, which comprises the following components in percentage by weight: 73-82% of ethanol, 0.1-0.16% of hydrogen peroxide, 0.5-2.0% of glycerol, 0.02-1% of synergistic component and the balance of deionized water. Wherein, the synergistic component is selected from one or more of dehydroacetic acid, sorbic acid, citric acid, lactic acid and the like, and is preferably citric acid or lactic acid.
Preferably, the no-clean quick-drying hand sanitizer capable of killing viruses in the embodiment of the invention comprises the following components in percentage by weight: 73-82% of ethanol, 0.1-0.16% of hydrogen peroxide, 0.5-2.0% of glycerol, 0.07-0.3% of synergistic component and the balance of deionized water. Wherein the synergistic component is selected from one or more of citric acid and lactic acid.
More preferably, the no-clean quick-drying hand sanitizer capable of killing viruses in the embodiment of the present invention is composed of the following components by weight: 75% of ethanol, 0.1% of hydrogen peroxide, 1.0% of glycerol, 0.25% of citric acid and the balance of deionized water.
More preferably, the no-clean quick-drying hand sanitizer capable of killing viruses in the embodiment of the present invention is composed of the following components by weight: 78.7% of ethanol, 0.14% of hydrogen peroxide, 1.45% of glycerol, 0.21% of lactic acid and the balance of deionized water.
The disinfectant provided by the invention has the following beneficial effects:
(1) acts for 1 minute according to the requirements of disinfection and sanitation standards, has an average inactivation logarithm value of more than 4 for the poliovirus type 1 vaccine strain, and has good inactivation effect on viruses. Under the same conditions, the combination of ethanol, hydrogen peroxide and glycerol has a killing logarithm value of 2-3, and citric acid, lactic acid and the like have no killing effect on poliovirus type 1 vaccine strains.
(2) The disinfectant can kill intestinal pathogenic bacteria, pyogenic coccus, pathogenic yeast, nosocomial infection common bacteria, etc.
(3) The disinfectant can inactivate virus under hand cleaning condition.
(4) The added synergistic components do not affect the stability of the hydrogen peroxide.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, embodiments of the present invention are described in further detail below.
Example one
The no-clean quick-drying hand sanitizer capable of killing viruses in the first embodiment comprises the following components in percentage by weight: 78.7% of ethanol, 0.14% of hydrogen peroxide, 1.45% of glycerol, 0.21% of lactic acid and the balance of deionized water.
Example two
The no-clean quick-drying hand sanitizer capable of killing viruses in the second embodiment comprises the following components in percentage by weight: 75% of ethanol, 0.1% of hydrogen peroxide, 1.0% of glycerol, 0.25% of citric acid and the balance of deionized water.
EXAMPLE III
The no-clean quick-drying hand sanitizer capable of killing viruses in the third embodiment comprises the following components in percentage by weight: 80% of ethanol, 0.15% of hydrogen peroxide, 0.8% of glycerol, 0.5% of sorbic acid and the balance of deionized water.
Example four
The no-clean quick-drying hand sanitizer capable of killing viruses in the fourth embodiment comprises the following components in percentage by weight: 78.5% of ethanol, 0.135% of hydrogen peroxide, 1.0% of glycerol, 0.7% of dehydroacetic acid and the balance of deionized water.
Verification example, the disinfectant provided in example 1 was used for all the tests.
First, sterilization test
1.1. Test strains: escherichia coli 8099, Staphylococcus aureus ATC6538 and Pseudomonas aeruginosa ATCC15442 were provided by food safety culture Collection of food microbial safety engineering research and development center of Guangdong province, and their 5 th generation fresh slant culture was used for the test.
1.2. Neutralizing agent: 3% Tween 80+0.3% lecithin +0.1% sodium thiosulfate in 0.03mol/L PBS.
1.3. Culture medium: tryptone soy agar medium.
1.4. Organic interfering substances: 3% BSA solution.
1.5. The method comprises the following steps: according to specification on disinfection (2002 edition) 2.1.1.5, 2.1.1.7. Neutralization test: the test bacteria is Escherichia coli, the sterilization time is 0.5min, the test environment temperature is 19-21 deg.C, the relative humidity is 45-60%, and the test is repeated for 3 times. And (3) sterilization test: sterilizing with disinfectant, testing at 19-21 deg.C and 45-60% relative humidity, and repeating for 3 times. The incubation temperature was 37 ℃.
1.6. Positive control: the test procedure was followed using sterile diluent instead of test solution.
1.7. Coli neutralizer identification test, the results are shown in table 1:
TABLE 1
Figure 146327DEST_PATH_IMAGE001
As can be seen from table 1: the average number of recovered bacteria in group 1 was 0CFU/mL, and the average number of recovered bacteria in group 2 was 207 CFU/mL. Therefore, the disinfectant has a killing effect on escherichia coli, and the disinfectant and a neutralization product have no adverse effect on the escherichia coli.
1.8. Killing test of test bacteria: the test environment temperature is 19-21 ℃, the test is repeated for 3 times, the killing logarithm values of the disinfectant on escherichia coli, staphylococcus aureus and pseudomonas aeruginosa are all more than 5.00 after the disinfectant is respectively acted for 0.5min, 1min and 1.5min, and the results are shown in tables 2-4:
TABLE 2
Figure 345227DEST_PATH_IMAGE002
TABLE 3
Figure 14105DEST_PATH_IMAGE003
TABLE 4
Figure 69786DEST_PATH_IMAGE004
Second, fungus killing test (suspension method)
2.1. Test strains: candida albicans (ATCC 10231) provided by food safety culture collection of research and development center of food and microorganism safety engineering, Guangdong province, and its 5 th generation fresh slant culture was used for testing.
2.2. Neutralizing agent: 3% Tween 80+0.3% lecithin +0.1% sodium thiosulfate in 0.03mol/L PBS.
2.3. Culture medium: sabouraud agar medium.
2.4. Organic interfering substances: 3% BSA solution.
2.5. The method comprises the following steps: according to specification on disinfection (2002 edition) 2.1.1.5, 2.1.1.9. And (3) neutralization test: the sterilization time is 0.5min, the test environment temperature is 19-21 ℃, the relative humidity is 45-60%, and the test is repeated for 3 times. And (3) sterilization test: sterilizing with disinfectant, testing at 19-21 deg.C and 45-60% relative humidity, and repeating for 3 times. The incubation temperature was 37 ℃.
2.6. Positive control: the test procedure was followed using sterile diluent instead of test solution. The results are shown in Table 5:
TABLE 5
Figure 824116DEST_PATH_IMAGE005
As can be seen from table 5: the average number of recovered bacteria in group 1 was 0CFU/mL, the average number of recovered bacteria in group 2 was 450CFU/mL, and group 6 was grown aseptically.
2.7. The killing effect on experimental bacteria is as follows: the experimental environment temperature is 19-21 ℃, the experiment is repeated for 3 times, the killing log values of the disinfectant on the candida albicans are all more than 5.00 in 0.5min, 1min and 1.5min, and the results are shown in table 6:
TABLE 6
Figure 371247DEST_PATH_IMAGE006
Thirdly, the disinfectant is tested on the hand disinfection site (sanitary hand)
3.1. Neutralizing agent: 3% Tween 80+0.3% lecithin +0.1% sodium thiosulfate in 0.03mol/L PBS.
3.2. Diluent agent: 0.03mol/L phosphate buffer solution of 0.1% Tween 80, pH 7.2.
3.3. Culture medium: tryptone soy agar medium.
3.4. The method comprises the following steps: according to specification on Sterilization (2002 edition) 2.1.2.6. And (3) disinfection test: using disinfectant as a use sample, wiping and disinfecting for 1min, wherein the test environment temperature is 19-21 ℃, and the relative humidity is 45-60%. A subject: two hands (sanitary hands) of 30 volunteers were sampled from the left hand before sterilization to serve as a positive control group, and the samples were applied to the right hand of the volunteer for 1min and then the samples were used as a test group. The sampling method comprises the following steps: and (3) wiping the sterile cotton swab in a sampling liquid test tube for wetting, smearing and sampling the positive control group and the disinfection test group, cutting a sampling end of the cotton swab into 10mL of sampling liquid in a sterile mode, and eluting. Negative control: directly culturing the sampling solution and the diluent in the same batch. The results are shown in Table 7:
TABLE 7
Figure 894632DEST_PATH_IMAGE007
As can be seen from the results in Table 7, the average killing log value is greater than 1.80, which meets the requirements of the Disinfection technical Specification, and the average killing log value is greater than 1.00.
Fourth, the disinfectant is tested on the hand disinfection field (surgical hand)
4.1. Neutralizing agent: 3% Tween 80+0.3% lecithin +0.1% sodium thiosulfate in 0.03mol/L PBS.
4.2. Diluent (b): 0.03mol/L phosphate buffer solution of 0.1% Tween 80, pH 7.2.
4.3. Culture medium: tryptone soy agar medium.
4.4. The method comprises the following steps: according to specification on Sterilization (2002 edition) 2.1.2.6. And (3) disinfection test: using disinfectant as a use sample, wiping and disinfecting for 3min, wherein the test environment temperature is 19-21 ℃, and the relative humidity is 45-60%. A subject: two hands (surgical hands) of 30 volunteers were sampled from the left hand before sterilization to serve as a positive control group, and the samples were applied to the right hand of the volunteer for 3min and then used as a test group. The sampling method comprises the following steps: and (3) wiping the sterile cotton swab in a sampling liquid test tube for wetting, smearing and sampling the positive control group and the disinfection test group, cutting a sampling end of the cotton swab into 10mL of sampling liquid in a sterile mode, and eluting. Negative control: directly culturing the sampling solution and the diluent in the same batch. The results are shown in Table 8:
TABLE 8
Figure 324477DEST_PATH_IMAGE008
As can be seen from the results in Table 8, the average kill log value is greater than 1.88, which meets the requirements of the Disinfection technical Specification, and the average kill log value is greater than 1.00.
Fifthly, quantitative inactivation test of poliovirus suspension
First, equipment
1. Test virus strains: poliovirus type I (PV-I) vaccine strain.
2. Host cell: VERO cells.
3. The disinfectant of example 1.
4. Neutralizing agent: D/E neutralizes the broth.
5. Cell culture bottles and 96-well culture plates.
6. Organic interfering substances: 0.3% bovine serum albumin.
7. Standard hard water (hardness 342 mg/L).
8. Cell maintenance medium, cell complete medium and fetal bovine serum.
9. A thermostat, a carbon dioxide incubator, a biological safety cabinet, an adjustable pipettor and sterile equipment.
Second, method
1. The detection basis is as follows: specification for Disinfection (2002 edition) 2.1.1.10.5 and 2.1.1.10.7.
2. Preparation of virus suspension: titer for the assay was 106TCID50/0.1mL-107TCID500.1mL ridgeThe poliovirus I type vaccine strain virus suspension is diluted in duplicate with 0.3% bovine serum albumin organic interfering substance, and is placed at a constant temperature of 20 ℃ for later use.
3. And (3) identification test of a neutralizer: the disinfectant is acted for 0.5min, and the test temperature is constant at 20 ℃. The experiment was repeated 3 times.
4. Virus inactivation test: the disinfectant solution is applied for 0.5min, 1.0min and 1.5 min. The test temperature was a constant temperature of 20 ℃. The test was repeated 3 times.
5. Detecting the ambient temperature: 20.5-21.0 ℃; relative humidity: 10 to 44 percent.
Three, result in
1. Neutralizer identification test
After 3 times of repeated tests, under the condition of a constant temperature test at 20 ℃, the identification result of the disinfectant neutralizer is as follows: the average titer value of the group 1 virus was 0.78, the average titer value of the group 2 virus was 2.50, the average titer values of the group 3, group 4 and group 5 viruses were 6.94, 6.78 and 7.11, respectively, and the group 6 was a negative control group, and the results are shown in table 9:
TABLE 9
Figure 628419DEST_PATH_IMAGE009
2. Inactivating effect on poliovirus
After 3 repeated tests, under the condition of a constant temperature test at 20 ℃, the disinfectant is applied for 1.0min, the average inactivation log value of the poliovirus is more than 4.00, and the results are shown in table 10:
watch 10
Figure 903543DEST_PATH_IMAGE010
Fourth, conclusion
1. After 3 times of repeated tests, under the condition of a constant temperature test at 20 ℃, the neutralizing agent solution of the D/E neutralizing broth can effectively neutralize the residual action of the disinfectant on the poliovirus type I vaccine strain, and the neutralizing agent and a neutralization product basically have no influence on the growth of the poliovirus type I vaccine strain and cells.
2. After repeated tests for 3 times, under the condition of a constant temperature test at 20 ℃, the disinfection solution is applied for 1.0min, and the average inactivation log value of the poliovirus type I vaccine strain is more than 4.00.
Sixthly, measuring the stability of the hydrogen peroxide (chemical method)
First, equipment
1. Sample preparation: the disinfectant of example 1.
2. An instrument device: 25mL brown polytetrafluoro burette, volumetric flask, etc., Shanghai Bo news SPX-250B-Z constant temperature incubator.
3. The concentration of potassium permanganate standard titration solution is 0.0200 mol/L.
Second, method
1. The detection basis is as follows: specification for Disinfection (2002 edition) 2.2.3.2 and 2.2.1.2.4.
2. Storage conditions are as follows: the sample was well sealed and placed in a 37 ℃ incubator for 3 months (90 d).
3. The test environment temperature is 20.5 ℃, and the relative humidity is 65.0%.
Three, result in
One sample batch was tested twice for each batch and the results are shown in table 11:
TABLE 11
Figure 360063DEST_PATH_IMAGE011
The content of the effective component hydrogen peroxide before preservation is 0.140%, the content of the effective component hydrogen peroxide after preservation is 0.133%, and the content reduction rate of the hydrogen peroxide is 5% after the preservation for 3 months at 37 ℃.
Comparative example 1
The no-clean quick-drying hand sanitizer of comparative example 1 consists of the following components in percentage by weight: 78.7% of ethanol, 0.14% of hydrogen peroxide, 1.45% of glycerol and the balance of deionized water.
Comparative example 2
The no-clean quick-drying hand sanitizer of comparative example 2 consists of the following components in percentage by weight: 75% of ethanol, 0.1% of hydrogen peroxide, 1.0% of glycerol and the balance of deionized water.
The effects of examples 1-2 and comparative examples 1-2 were verified using the aforementioned poliovirus suspension quantitative inactivation assay, with an action time of 1.0 min. The results are shown in Table 12:
TABLE 12
Figure 695229DEST_PATH_IMAGE012
In addition, through tests, the citric acid or the lactic acid has no killing effect on the poliovirus basically; therefore, the citric acid or lactic acid can improve the effect of killing poliovirus when being matched with the conventional quick-drying hand disinfectant.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.

Claims (4)

1. A washing-free hand-drying disinfectant capable of killing viruses is characterized by comprising the following components in percentage by weight: 73-82% of ethanol, 0.1-0.16% of hydrogen peroxide, 0.5-2.0% of glycerol, 0.02-1% of synergistic component and the balance of deionized water, wherein the synergistic component is one or more selected from dehydroacetic acid, sorbic acid, citric acid and lactic acid.
2. The virus-killing no-clean hand-drying disinfectant as claimed in claim 1, which is prepared from the following components in percentage by weight: 73-82% of ethanol, 0.1-0.16% of hydrogen peroxide, 0.5-2.0% of glycerol, 0.07-0.3% of synergistic component and the balance of deionized water, wherein the synergistic component is selected from one or more of citric acid and lactic acid.
3. The virus-killing no-clean hand-drying disinfectant as claimed in claim 1, which is prepared from the following components in percentage by weight: 75% of ethanol, 0.1% of hydrogen peroxide, 1.0% of glycerol, 0.25% of citric acid and the balance of deionized water.
4. The virus-killing no-clean hand-drying disinfectant as claimed in claim 1, which is prepared from the following components in percentage by weight: 78.7% of ethanol, 0.14% of hydrogen peroxide, 1.45% of glycerol, 0.21% of lactic acid and the balance of deionized water.
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