CN114668023A - Natural antibacterial mildew preventive and macromolecular active antibacterial mildew-proof slow-release packaging film - Google Patents
Natural antibacterial mildew preventive and macromolecular active antibacterial mildew-proof slow-release packaging film Download PDFInfo
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Abstract
The invention specifically discloses a natural bacteriostatic mildew inhibitor and a high-molecular active bacteriostatic mildew-proof slow-release packaging film. The natural bacteriostatic mildew inhibitor comprises mustard essential oil, allicin and a eucalyptus leaf extract. The preparation method of the high-molecular active antibacterial mildew-proof slow-release packaging film comprises the following steps: (1) adsorbing the natural bacteriostatic mildew inhibitor in a carbon nano tube to prepare a carbon nano tube composite microcapsule; (2) then preparing the carbon nano tube composite microcapsule and paper pulp into composite active bacteriostatic mildew-proof slow-release paper; (3) the composite active antibacterial and mildewproof slow-release paper and the polymer film are prepared into the polymer active antibacterial and mildewproof slow-release packaging film by a hot-pressing composite method. The natural bacteriostatic mildew inhibitor has excellent bacteriostatic and mildew-proof effects; in addition, the active antibacterial mildew-proof slow-release packaging film prepared by the natural antibacterial mildew-proof agent by adopting the method can continuously release the natural antibacterial mildew-proof agent to generate continuous antibacterial mildew-proof effects.
Description
Technical Field
The invention relates to the technical field of antibacterial mildewcide and antibacterial packaging film, in particular to a natural antibacterial mildewcide and a high-molecular-activity antibacterial mildewproof slow-release packaging film.
Background
The mustard essential oil is obtained by steam distilling semen Brassicae Junceae, and has antibacterial effect. Allicin (Allicin), an organic sulfur compound extracted from the bulb (garlic bulb) of garlic, a plant belonging to the genus allium, the family alliaceae, is also present in onions and other plants of the family alliaceae, and is known under the chemical name of diallylthiosulfinate; it has inhibitory effect on various gram-positive and gram-negative cocci, fungi, viruses, protozoa, enterobiasis, etc. In the preparation process of the antibacterial and mildewproof packaging film, mustard essential oil and allicin are usually added into the packaging film material as antibacterial and mildewproof agents, so that the prepared packaging film has antibacterial and mildewproof effects.
Methicillin-resistant staphylococcus aureus (MRSA) is a clinically common bacterium with strong toxicity, and has become one of important pathogenic bacteria of nosocomial and community infections at present. However, researches show that mustard essential oil and allicin as the bacteriostatic and mildewproof agent can achieve better bacteriostatic and mildewproof effects; but its inhibitory effect on methicillin-resistant staphylococcus aureus (MRSA) was not significant. If the film or packaging film used in hospitals only uses mustard essential oil and allicin as bacteriostatic and mildewproof agents, the film or packaging film cannot inhibit methicillin-resistant staphylococcus aureus (MRSA). Therefore, the invention aims to solve the technical problem of how to improve the inhibition effect of the bacteriostatic mildewcide consisting of mustard essential oil and allicin on methicillin-resistant staphylococcus aureus (MRSA).
Disclosure of Invention
In order to overcome at least one technical problem in the prior art, the invention firstly provides a natural bacteriostatic mildew inhibitor; the natural bacteriostatic mildew inhibitor not only can play the bacteriostatic and mildew-proof effects of the mustard essential oil and the allicin, but also has the inhibitory effect on methicillin-resistant staphylococcus aureus (MRSA) after the eucalyptus leaf extract is added into the mustard essential oil and the allicin.
The technical problem to be solved by the invention is solved by the following technical scheme:
a natural bacteriostatic and antifungal agent comprises mustard essential oil, garlicin and folium Eucalypti Globueli extract.
Preferably, the weight ratio of the mustard essential oil to the allicin and the eucalyptus leaf extract is 10: 1-3.
Most preferably, the weight ratio of mustard essential oil to allicin and eucalyptus leaf extract is 10:1: 2.
Preferably, the eucalyptus leaf extract is prepared by the following method:
and (3) adding an organic solvent into the eucalyptus leaves for extraction, concentrating and drying the extract liquid after the extraction is finished to obtain the organic solvent extract of the eucalyptus leaves, namely the eucalyptus leaf extract.
Preferably, the organic solvent is a mixed organic solvent consisting of petroleum ether and acetone; wherein the volume ratio of the petroleum ether to the acetone is 3-5: 1.
The inventors have unexpectedly found in their studies that: after the eucalyptus leaf organic solvent extract obtained by extracting the mixed organic solvent consisting of petroleum ether and acetone is added into the antibacterial and antifungal agent consisting of mustard essential oil and allicin, the inhibition effect on methicillin-resistant staphylococcus aureus is far higher than that of the eucalyptus leaf organic solvent extract obtained by adding other organic solvents.
The mustard essential oil is prepared by taking yellow mustard seeds as raw materials and adopting a conventional steam distillation method.
Preferably, the extraction is heating reflux extraction, and the extraction time is 0.5-2 h.
Preferably, the extraction is heating reflux extraction, and the extraction time is 1-2 h.
Preferably, the using amount ratio of the eucalyptus leaves to the organic solvent is 1g: 6-12 mL.
Most preferably, the amount ratio of the eucalyptus leaves to the organic solvent is 1g:8 mL.
Preferably, the eucalyptus leaf organic solvent extract is further applied to a silica gel column, and organic solvent composed of chloroform and methanol with the volume fraction of 97-99: 3-1 and the volume fraction of 3-6 times of the column volume is used for eluting and removing impurities; then eluting with 5-8 times of column volume of organic solvent composed of chloroform and methanol with volume fraction of 93-95: 7-5, collecting eluent eluted by the organic solvent composed of chloroform and methanol with volume fraction of 93-95: 7-5, concentrating and drying to obtain the eucalyptus leaf extract.
Most preferably, the eucalyptus leaf organic solvent extract is further applied to a silica gel column, and is eluted and decontaminated by an organic solvent which is 5 times of the column volume and consists of chloroform and methanol with the volume fraction of 98: 2; then eluting with 6 times of column volume of organic solvent composed of chloroform and methanol with volume fraction of 94:6, collecting eluate eluted with organic solvent composed of chloroform and methanol with volume fraction of 94:6, concentrating, and drying to obtain folium Eucalypti Globueli extract.
In order to further improve the inhibition effect of the natural bacteriostatic mildewcide consisting of mustard essential oil, allicin and eucalyptus leaf extract on methicillin-resistant staphylococcus aureus; the inventor finds out through a large number of experiments that: the natural bacteriostatic mildew inhibitor consisting of the eucalyptus leaf extract prepared under the silica gel column elution condition is added into the mustard essential oil and the allicin, so that the inhibition effect on methicillin-resistant staphylococcus aureus is greatly improved.
The inventor also finds in research that the inhibition effect of the eucalyptus leaf extract on the methicillin-resistant staphylococcus aureus can be improved without adding the natural bacteriostatic mildew-resistant agent consisting of the eucalyptus leaf extract prepared under the condition of random silica gel column elution and the mustard essential oil and the allicin. The eucalyptus leaf extract prepared under different silica gel column elution conditions is added into the mustard essential oil and the allicin to form the natural bacteriostatic mildewcide, and the difference of the inhibition effect of the natural bacteriostatic mildewcide on the methicillin-resistant staphylococcus aureus is great; research shows that compared with the method of adding the eucalyptus leaf organic solvent extract into the mustard essential oil and the allicin, the natural bacteriostatic mildewcide formed by adding the eucalyptus leaf extract prepared under the silica gel column elution condition into the mustard essential oil and the allicin has more excellent inhibitory action on methicillin-resistant staphylococcus aureus. The natural bacteriostatic mildew inhibitor formed by adding the eucalyptus leaf extract prepared under the elution condition of other silica gel columns into mustard essential oil and allicin can not greatly improve or even reduce the inhibition effect on methicillin-resistant staphylococcus aureus.
The invention also provides an application of the natural bacteriostatic mildew-proof agent in preparing a film with bacteriostatic and mildew-proof effects.
The natural bacteriostatic mildew inhibitor is used in the packaging film, so that the prepared packaging film has a bacteriostatic action on conventional mould and bacteria; and also has inhibitory effect on methicillin-resistant Staphylococcus aureus.
The invention also provides a high-molecular active antibacterial and mildewproof slow-release packaging film, and the antibacterial and mildewproof agent in the high-molecular active antibacterial and mildewproof slow-release packaging film is the natural antibacterial and mildewproof agent.
Preferably, the preparation method of the high molecular activity antibacterial and mildewproof slow-release packaging film comprises the following steps:
(1) adsorbing the natural bacteriostatic mildew inhibitor in a carbon nano tube to prepare a carbon nano tube composite microcapsule;
(2) then preparing the carbon nano tube composite microcapsule and paper pulp into composite active bacteriostatic mildew-proof slow-release paper;
(3) the composite active antibacterial mildew-proof slow-release paper and a polymer film (PE PP PET and the like) are prepared into the polymer active antibacterial mildew-proof slow-release packaging film by a hot-pressing compounding method.
The inventor further researches and discovers that the active antibacterial and mildewproof slow-release packaging film prepared by the natural antibacterial and mildewproof agent by the method can continuously release the natural antibacterial and mildewproof agent to generate continuous antibacterial and mildewproof effects.
Has the advantages that: (1) the invention firstly provides a brand-new natural bacteriostatic mildew inhibitor; the natural bacteriostatic mildew inhibitor not only can play the bacteriostatic and mildew-proof effects of the mustard essential oil and the allicin, but also has the inhibitory effect on methicillin-resistant staphylococcus aureus. (2) The natural bacteriostatic mildew inhibitor is used in the packaging film, so that the prepared packaging film has a bacteriostatic action on conventional mould and bacteria; simultaneously, the product also has an inhibiting effect on methicillin-resistant staphylococcus aureus; particularly, when the film or the packaging film is used in hospitals, the methicillin-resistant staphylococcus aureus can be effectively inhibited, so that the spread of the methicillin-resistant staphylococcus aureus in the hospitals is reduced. (3) The active antibacterial mildew-proof slow-release packaging film prepared by the method can continuously release the natural antibacterial mildew-proof agent to generate continuous antibacterial mildew-proof effects.
Detailed Description
The present invention will be further explained with reference to specific examples, which are not intended to limit the present invention in any way.
EXAMPLE 1 preparation of Natural bacteriostatic mildewcide
Uniformly mixing mustard essential oil, allicin and a eucalyptus leaf extract according to a weight ratio of 10:1:2 to obtain the natural bacteriostatic mildew inhibitor;
the eucalyptus leaf extract is prepared by the following method: taking eucalyptus leaves, adding an organic solvent, heating, refluxing and extracting for 1h, concentrating and drying the extract liquid after extraction is finished to obtain an organic solvent extract of the eucalyptus leaves, namely the eucalyptus leaf extract;
wherein the dosage ratio of the eucalyptus leaves to the organic solvent is 1g to 8 mL; the organic solvent consists of petroleum ether and acetone in a volume ratio of 4: 1.
Example 2 preparation of Natural bacteriostatic mildewcide
Uniformly mixing mustard essential oil, allicin and a eucalyptus leaf extract according to a weight ratio of 10:3:1 to obtain the natural bacteriostatic mildew inhibitor;
the eucalyptus leaf extract is prepared by the following method: taking eucalyptus leaves, adding an organic solvent, heating, refluxing and extracting for 1h, concentrating and drying an extract solution after extraction is finished to obtain an organic solvent extract of the eucalyptus leaves, namely the eucalyptus leaf extract;
wherein the dosage ratio of the eucalyptus leaves to the organic solvent is 1g:12 mL; the organic solvent consists of petroleum ether and acetone in a volume ratio of 3: 1.
EXAMPLE 3 preparation of Natural bacteriostatic mildewcide
Uniformly mixing mustard essential oil, allicin and the eucalyptus leaf extract according to the weight ratio of 10:1:3 to obtain the natural bacteriostatic mildew inhibitor;
the eucalyptus leaf extract is prepared by the following method: taking eucalyptus leaves, adding an organic solvent, heating, refluxing and extracting for 1h, concentrating and drying an extract solution after extraction is finished to obtain an organic solvent extract of the eucalyptus leaves, namely the eucalyptus leaf extract;
wherein the dosage ratio of the eucalyptus leaves to the organic solvent is 1g:6 mL; the organic solvent consists of petroleum ether and acetone in a volume ratio of 5: 1.
Example 4 preparation of Natural bacteriostatic mildewcide
Uniformly mixing mustard essential oil, allicin and a eucalyptus leaf extract according to a weight ratio of 10:1:2 to obtain the natural bacteriostatic mildew inhibitor;
the eucalyptus leaf extract is prepared by the following method:
(1) taking eucalyptus leaves, adding an organic solvent, heating, refluxing and extracting for 1h, and concentrating and drying an extract liquid after extraction is finished to obtain an organic solvent extract of the eucalyptus leaves; wherein the dosage ratio of the eucalyptus leaves to the organic solvent is 1g:8 mL; the organic solvent consists of petroleum ether and acetone in a volume ratio of 4: 1;
(2) applying a silica gel column (200-mesh 300-mesh silica gel is adopted, the weight of the silica gel is 25 times of that of the organic solvent extract of the eucalyptus leaves), and eluting by using an organic solvent which is 5 times of the column volume and consists of chloroform and methanol with the volume fraction of 98:2 to remove impurities; then eluting with 6 times of column volume of organic solvent composed of chloroform and methanol with volume fraction of 94:6, collecting eluate eluted with organic solvent composed of chloroform and methanol with volume fraction of 94:6, concentrating, and drying to obtain folium Eucalypti Globueli extract.
Comparative example 1 preparation of Natural bacteriostatic mildewproof agent
Uniformly mixing mustard essential oil and allicin according to the weight ratio of 10:1 to obtain the natural bacteriostatic mildew inhibitor;
comparative example 1 differs from example 1 in that the natural bacteriostatic mildewcide of comparative example 1 is obtained only from a combination of mustard essential oil and allicin. The natural bacteriostatic mildew inhibitor described in the embodiment 1 is prepared by combining mustard essential oil, allicin and an extract of eucalyptus leaves.
Comparative example 2 preparation of Natural bacteriostatic mildew preventive
Uniformly mixing mustard essential oil, allicin and a eucalyptus leaf extract according to a weight ratio of 10:1:2 to obtain the natural bacteriostatic mildew inhibitor;
the eucalyptus leaf extract is prepared by the following method: taking eucalyptus leaves, adding an organic solvent, heating, refluxing and extracting for 1h, concentrating and drying an extract solution after extraction is finished to obtain an organic solvent extract of the eucalyptus leaves, namely the eucalyptus leaf extract;
wherein the dosage ratio of the eucalyptus leaves to the organic solvent is 1g:8 mL; the organic solvent is petroleum ether.
Comparative example 2 differs from example 1 in that the eucalyptus leaf extract of comparative example 2 is obtained by extraction with petroleum ether only; the eucalyptus leaf extract described in example 1 is prepared by using a mixed solvent composed of petroleum ether and acetone.
Comparative example 3 preparation of Natural bacteriostatic mildew preventive
Uniformly mixing mustard essential oil, allicin and a eucalyptus leaf extract according to a weight ratio of 10:1:2 to obtain the natural bacteriostatic mildew inhibitor;
the eucalyptus leaf extract is prepared by the following method: taking eucalyptus leaves, adding an organic solvent, heating, refluxing and extracting for 1h, concentrating and drying an extract solution after extraction is finished to obtain an organic solvent extract of the eucalyptus leaves, namely the eucalyptus leaf extract;
wherein the dosage ratio of the eucalyptus leaves to the organic solvent is 1g:8 mL; the organic solvent is acetone.
Comparative example 3 is different from example 1 in that the eucalyptus leaf extract of comparative example 3 is obtained by extracting only acetone; the eucalyptus leaf extract as described in example 1 is prepared by using a mixed solvent consisting of petroleum ether and acetone.
Comparative example 4 preparation of Natural bacteriostatic mildewproof agent
Uniformly mixing mustard essential oil, allicin and a eucalyptus leaf extract according to a weight ratio of 10:1:2 to obtain the natural bacteriostatic mildew inhibitor;
the eucalyptus leaf extract is prepared by the following method: taking eucalyptus leaves, adding an organic solvent, heating, refluxing and extracting for 1h, concentrating and drying an extract solution after extraction is finished to obtain an organic solvent extract of the eucalyptus leaves, namely the eucalyptus leaf extract;
wherein the dosage ratio of the eucalyptus leaves to the organic solvent is 1g:8 mL; the organic solvent is methanol.
Comparative example 4 is different from example 1 in that the eucalyptus leaf extract in comparative example 4 is obtained by methanol extraction; the eucalyptus leaf extract as described in example 1 is prepared by using a mixed solvent consisting of petroleum ether and acetone.
Comparative example 5 preparation of Natural bacteriostatic mildew preventive
Uniformly mixing mustard essential oil, allicin and a eucalyptus leaf extract according to a weight ratio of 10:1:2 to obtain the natural bacteriostatic mildew inhibitor;
the eucalyptus leaf extract is prepared by the following method: taking eucalyptus leaves, adding an organic solvent, heating, refluxing and extracting for 1h, concentrating and drying an extract solution after extraction is finished to obtain an organic solvent extract of the eucalyptus leaves, namely the eucalyptus leaf extract;
wherein the dosage ratio of the eucalyptus leaves to the organic solvent is 1g to 8 mL; the organic solvent is ethanol with the volume fraction of 95%.
Comparative example 5 is different from example 1 in that the eucalyptus leaf extract of comparative example 5 is obtained by extracting with ethanol with a volume fraction of 95%; the eucalyptus leaf extract described in example 1 is prepared by using a mixed solvent composed of petroleum ether and acetone.
Comparative example 6 preparation of Natural bacteriostatic mildewproof agent
Uniformly mixing mustard essential oil, allicin and a eucalyptus leaf extract according to a weight ratio of 10:1:2 to obtain the natural bacteriostatic mildew inhibitor;
the eucalyptus leaf extract is prepared by the following method:
(1) taking eucalyptus leaves, adding an organic solvent, heating, refluxing and extracting for 1h, and concentrating and drying an extract liquid after extraction is finished to obtain an organic solvent extract of the eucalyptus leaves; wherein the dosage ratio of the eucalyptus leaves to the organic solvent is 1g:8 mL; the organic solvent consists of petroleum ether and acetone in a volume ratio of 4: 1;
(2) applying a silica gel column (200-mesh 300-mesh silica gel is adopted, the weight of the silica gel is 25 times of that of the organic solvent extract of the eucalyptus leaves), and eluting by using an organic solvent which is 5 times of the column volume and consists of chloroform and methanol with the volume fraction of 95:5 to remove impurities; then eluting with 6 times of column volume of organic solvent composed of chloroform and methanol with volume fraction of 85:15, collecting eluate eluted with organic solvent composed of chloroform and methanol with volume fraction of 85:15, concentrating, and drying to obtain folium Eucalypti Globueli extract.
Comparative example 6 is different from example 4 in that the silica gel column elution conditions during the preparation of the eucalyptus leaf extract are different. The silica gel column elution conditions of comparative example 6 were: eluting and removing impurities by using an organic solvent consisting of chloroform and methanol with the volume fraction of 95: 5; then eluting with an organic solvent consisting of chloroform and methanol with the volume fraction of 85: 15; and the silica gel column elution conditions of example 4 were: eluting and removing impurities by using an organic solvent consisting of chloroform and methanol with the volume fraction of 98: 2; then, the mixture was eluted with an organic solvent consisting of chloroform and methanol at a volume fraction of 94: 6.
Comparative example 7 preparation of Natural bacteriostatic mildew preventive
Uniformly mixing mustard essential oil, allicin and the eucalyptus leaf extract according to the weight ratio of 10:1:2 to obtain the natural bacteriostatic mildew inhibitor;
the eucalyptus leaf extract is prepared by the following method:
(1) taking eucalyptus leaves, adding an organic solvent, heating, refluxing and extracting for 1h, and concentrating and drying an extract liquid after extraction is finished to obtain an organic solvent extract of the eucalyptus leaves; wherein the dosage ratio of the eucalyptus leaves to the organic solvent is 1g:8 mL; the organic solvent consists of petroleum ether and acetone in a volume ratio of 4: 1;
(2) applying a silica gel column (200-mesh 300-mesh silica gel is adopted, the weight of the silica gel is 25 times of that of the organic solvent extract of the eucalyptus leaves), and eluting by using an organic solvent which is 5 times of the column volume and consists of chloroform and methanol with the volume fraction of 90:10 to remove impurities; then eluting with organic solvent composed of chloroform and methanol with volume fraction of 80:20 of 6 times of column volume, collecting eluate eluted with organic solvent composed of chloroform and methanol with volume fraction of 80:20, concentrating, and drying to obtain folium Eucalypti Globueli extract.
Comparative example 7 is different from example 4 in that the silica gel column elution conditions during the preparation of the eucalyptus leaf extract are different. The silica gel column elution conditions of comparative example 7 were: eluting and removing impurities by using an organic solvent consisting of chloroform and methanol with the volume fraction of 90: 10; then eluting with an organic solvent consisting of chloroform and methanol with the volume fraction of 80: 20; and the silica gel column elution conditions of example 4 were: eluting and removing impurities by using an organic solvent consisting of chloroform and methanol with the volume fraction of 98: 2; then, the mixture was eluted with an organic solvent consisting of chloroform and methanol at a volume fraction of 94: 6.
Experimental example 1 methicillin-resistant Staphylococcus aureus (MRSA) bacteriostasis test objects are the natural bacteriostasis and mildew preventive agents prepared in examples 1-4 and comparative examples 1-7.
The natural bacteriostatic mildew preventive to be detected is respectively diluted into liquid medicines to be detected of 40mg/mL, 20mg/mL, 10mg/mL, 5mg/mL, 2.5mg/mL, 1.25mg/mL and 0.625mg/mL by a double dilution method. After a beef extract peptone culture medium is melted, putting 19mL of the beef extract peptone culture medium into culture dishes, then respectively adding 1mL of liquid medicine to be detected with different concentrations into each culture dish, and uniformly mixing; after the culture medium is cooled and solidified, carrying out photocopy on methicillin-resistant staphylococcus aureus (MRSA) in culture dishes containing medicaments to be detected with different concentrations by adopting a photocopy method, then putting the culture dishes into an incubator, and carrying out constant-temperature culture at 37 ℃ for 24 hours; observing the growth condition of the strain after the culture is finished, and recording the concentration at which the strain does not grow as the minimum inhibitory concentration; the test results are shown in Table 1.
TABLE 1 minimum inhibitory concentration of the natural bacteriostatic mildewcide of the invention on MRSA
| Minimum inhibitory concentration for MRSA | |
| The natural bacteriostatic and mildewproof agent of example 1 | 10mg/mL |
| The natural bacteriostatic mildewcide of example 2 | 10mg/mL |
| The natural bacteriostatic mildewcide of example 3 | 10mg/mL |
| The natural bacteriostatic and mildewproof agent of example 4 | 1.25mg/mL |
| The natural bacteriostatic mildew-proof agent of comparative example 1 | - |
| The natural bacteriostatic mildew inhibitor described in comparative example 2 | 20mg/mL |
| Comparative example 3 instituteThe natural bacteriostatic mildew inhibitor | 20mg/mL |
| The natural bacteriostatic mildew inhibitor described in comparative example 4 | 40mg/mL |
| The natural bacteriostatic mildew inhibitor of comparative example 5 | 40mg/mL |
| The natural bacteriostatic mildew-proof agent of comparative example 6 | 40mg/mL |
| The natural bacteriostatic mildew-proof agent of comparative example 7 | - |
As can be seen from the experimental data in Table 1, comparative example 1, which is a natural bacteriostatic and antifungal agent consisting of only mustard essential oil and allicin, shows no inhibitory effect on methicillin-resistant Staphylococcus aureus (MRSA) in the above concentration range; this indicates that: the natural bacteriostatic mildewcide only consisting of mustard essential oil and allicin has no obvious inhibition effect on methicillin-resistant staphylococcus aureus (MRSA). However, the natural bacteriostatic mildewcide of the embodiments 1 to 3 has the minimum bacteriostatic concentration of 10mg/mL to methicillin-resistant staphylococcus aureus (MRSA), and has an obvious inhibitory effect on methicillin-resistant staphylococcus aureus (MRSA). This indicates that: the natural bacteriostatic mildew inhibitor obtained by adding the eucalyptus leaf organic solvent extract into the mustard essential oil and the allicin has obvious inhibition effect on methicillin-resistant staphylococcus aureus (MRSA).
As can be seen from the experimental data in Table 1, the minimum inhibitory concentration of the natural bacteriostatic mildewcide in the comparative examples 2 to 5 to methicillin-resistant Staphylococcus aureus (MRSA) is far higher than that of the natural bacteriostatic mildewcide in the examples 1 to 3. This indicates that: the natural bacteriostatic mildew inhibitor is prepared by adding different organic solvents into mustard essential oil and allicin to extract eucalyptus leaf organic solvent extracts, and has great difference in inhibition effect on methicillin-resistant staphylococcus aureus (MRSA); the above studies show that: the natural bacteriostatic mildew inhibitor is prepared by adding a eucalyptus leaf organic solvent extract prepared by extracting mixed organic solvents consisting of petroleum ether and acetone into mustard essential oil and allicin, and the inhibition effect on methicillin-resistant staphylococcus aureus is far higher than that of the natural bacteriostatic mildew inhibitor prepared by adding the eucalyptus leaf organic solvent extract prepared by extracting other organic solvents; and the natural bacteriostatic mildew inhibitor which has obvious inhibition effect on methicillin-resistant staphylococcus aureus (MRSA) can be prepared only by adding the eucalyptus leaf organic solvent extract obtained by extracting the mixed organic solvent consisting of petroleum ether and acetone into the mustard essential oil and the allicin.
As can be seen from the experimental data in Table 1, the minimum inhibitory concentration of the bacteriostatic and mildewproof agent in the embodiment 4 is 1.25mg/mL, which is far less than 10mg/mL of the bacteriostatic and mildewproof agent in the embodiments 1 to 3; this indicates that: adding a natural bacteriostatic mildew inhibitor consisting of the eucalyptus leaf extract prepared under the silica gel column elution condition into mustard essential oil and allicin; compared with the added eucalyptus leaf organic solvent extract, the inhibition effect of the natural antibacterial mildew preventive on methicillin-resistant staphylococcus aureus (MRSA) can be further greatly improved.
As can be seen from the experimental data in table 1, the minimum inhibitory concentration of the bacteriostatic mildewcide in the comparative example 6 is 40mg/mL, which is much higher than that in the example 4 and is also much higher than that in the comparative example 1; and the bacteriostatic and mildewproof agent of comparative example 7 showed no inhibitory effect on methicillin-resistant staphylococcus aureus (MRSA) in the above concentration range. This indicates that: the natural bacteriostatic mildew inhibitor which is formed by adding the eucalyptus leaf extract prepared under the condition of random silica gel column elution into mustard essential oil and allicin has obvious inhibition effect on methicillin-resistant staphylococcus aureus (MRSA). The eucalyptus leaf extract prepared under different silica gel column elution conditions is added into mustard essential oil and allicin to form a natural bacteriostatic mildew inhibitor, and the difference of the inhibition effect of the natural bacteriostatic mildew inhibitor on methicillin-resistant staphylococcus aureus (MRSA) is great; the research shows that: compared with the addition of the eucalyptus leaf organic solvent extract into the mustard essential oil and the allicin, the natural bacteriostatic mildewproof agent consisting of the eucalyptus leaf extract prepared under the silica gel column elution condition (namely, the organic solvent consisting of chloroform and methanol with the volume fraction of 97-99: 3-1 is used for eluting and impurity removal first, and then the organic solvent consisting of chloroform and methanol with the volume fraction of 93-95: 7-5 is used for eluting) is added into the mustard essential oil and the allicin, so that the inhibition effect of the natural bacteriostatic mildewproof agent on methicillin-resistant staphylococcus aureus (MRSA) can be further greatly improved, and the more excellent inhibition effect can be achieved; the natural bacteriostatic mildew inhibitor formed by adding the eucalyptus leaf extract prepared under the elution condition of other silica gel columns into mustard essential oil and allicin can not greatly improve or even reduce the inhibition effect on methicillin-resistant staphylococcus aureus (MRSA).
Example 5 preparation of high-molecular active antibacterial and mildewproof sustained-release packaging film
(1) Adsorbing the natural bacteriostatic mildew inhibitor prepared in the embodiment 1 in a carbon nano tube to prepare a carbon nano tube composite microcapsule;
(2) then preparing the carbon nano tube composite microcapsule and paper pulp into composite active bacteriostatic and mildewproof slow-release paper;
(3) the composite active antibacterial and mildewproof slow-release paper and the PE high polymer film are prepared into the high polymer active antibacterial and mildewproof slow-release packaging film by a hot-pressing composite method.
Example 6 preparation of high-molecular active antibacterial and mildewproof sustained-release packaging film
(1) Adsorbing the natural bacteriostatic mildew inhibitor prepared in the embodiment 2 in a carbon nano tube to prepare a carbon nano tube composite microcapsule;
(2) then preparing the carbon nano tube composite microcapsule and paper pulp into composite active bacteriostatic and mildewproof slow-release paper;
(3) the composite active antibacterial and mildewproof slow-release paper and the PP high-molecular film are prepared into the high-molecular active antibacterial and mildewproof slow-release packaging film by a hot-pressing composite method.
Example 7 preparation of polymeric active bacteriostatic mildewproof sustained-release packaging film
(1) Adsorbing the natural bacteriostatic mildew inhibitor prepared in the embodiment 4 in a carbon nano tube to prepare a carbon nano tube composite microcapsule;
(2) then preparing the carbon nano tube composite microcapsule and paper pulp into composite active bacteriostatic mildew-proof slow-release paper;
(3) the composite active antibacterial and mildewproof slow-release paper and the PET polymer film are prepared into the polymer active antibacterial and mildewproof slow-release packaging film by a hot-pressing composite method.
Claims (10)
1. A natural bacteriostatic mildew inhibitor is characterized by comprising mustard essential oil, allicin and a eucalyptus leaf extract.
2. The natural bacteriostatic and mildewproof agent according to claim 1, wherein the weight ratio of the mustard essential oil to the allicin to the eucalyptus leaf extract is 10: 1-3.
3. The natural bacteriostatic and mildewproof agent according to claim 2, wherein the weight ratio of the mustard essential oil to the allicin to the eucalyptus leaf extract is 10:1: 2.
4. The natural bacteriostatic and mildewproof agent according to claim 1, wherein the eucalyptus leaf extract is prepared by the following steps:
and (3) adding an organic solvent into the eucalyptus leaves for extraction, concentrating and drying the extract liquid after the extraction is finished to obtain the organic solvent extract of the eucalyptus leaves, namely the eucalyptus leaf extract.
5. The natural bacteriostatic and mildewproof agent according to claim 4, wherein the organic solvent is a mixed organic solvent consisting of petroleum ether and acetone; wherein the volume ratio of the petroleum ether to the acetone is 3-5: 1.
6. The natural bacteriostatic and mildewproof agent according to claim 4, wherein the using amount ratio of the eucalyptus leaves to the organic solvent is 1 g/6-12 mL.
7. The natural bacteriostatic and mildewproof agent according to claim 6, wherein the dosage ratio of the eucalyptus leaves to the organic solvent is 1g to 8 mL.
8. Use of the natural bacteriostatic and mildewproof agent of any one of claims 1 to 7 in preparation of a film with bacteriostatic and mildewproof effects.
9. A high molecular activity antibacterial mildew-proof slow-release packaging film is characterized in that the antibacterial mildew-proof agent in the high molecular activity antibacterial mildew-proof slow-release packaging film is the natural antibacterial mildew-proof agent according to any one of claims 1 to 7.
10. The preparation method of the high molecular activity bacteriostatic and mildewproof slow-release packaging film according to claim 9, which is characterized by comprising the following steps of:
(1) adsorbing the natural bacteriostatic mildewcide according to any one of claims 1 to 7 in carbon nanotubes to prepare a carbon nanotube composite microcapsule;
(2) then preparing the carbon nano tube composite microcapsule and paper pulp into composite active bacteriostatic and mildewproof slow-release paper;
(3) the composite active antibacterial mildew-proof slow-release paper and a polymer film (PE PP PET and the like) are prepared into the polymer active antibacterial mildew-proof slow-release packaging film by a hot-pressing compounding method.
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