CN114653702B - Cell culture is intelligent cleaning device for accessory - Google Patents
Cell culture is intelligent cleaning device for accessory Download PDFInfo
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- CN114653702B CN114653702B CN202210538927.5A CN202210538927A CN114653702B CN 114653702 B CN114653702 B CN 114653702B CN 202210538927 A CN202210538927 A CN 202210538927A CN 114653702 B CN114653702 B CN 114653702B
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/08—Cleaning containers, e.g. tanks
- B08B9/093—Cleaning containers, e.g. tanks by the force of jets or sprays
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B13/00—Accessories or details of general applicability for machines or apparatus for cleaning
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B7/00—Cleaning by methods not provided for in a single other subclass or a single group in this subclass
- B08B7/02—Cleaning by methods not provided for in a single other subclass or a single group in this subclass by distortion, beating, or vibration of the surface to be cleaned
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B2209/00—Details of machines or methods for cleaning hollow articles
- B08B2209/08—Details of machines or methods for cleaning containers, e.g. tanks
- B08B2209/085—Cleaning flexible bags
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to the field of cell culture, in particular to intelligent cleaning equipment for cell culture accessories. The technical problem that the cleaning treatment difficulty of the inner wall of the cell culture bag is large due to the fact that a large number of cells remain on the inner wall of the cell culture bag is solved. The invention provides an intelligent cleaning device for cell culture accessories, which comprises a cell ejecting unit, a magnetic suction type extrusion unit and the like; the downside that the cell was popped off the unit is connected with magnetism and inhales formula extrusion unit. According to the invention, a layer of nutrient solution flowing layer is sprayed on the inner wall of the cell culture bag from top to bottom, meanwhile, each area of the cell culture bag is inwards extruded and sunken, the extruded area of the cell culture bag is outwards rebounded and collided with the cell elastic unit, so that the cells are vibrated and fall off, and the cells adhered to the inner wall of the cell culture bag are efficiently recovered by using a small amount of nutrient solution.
Description
Technical Field
The invention relates to the field of cell culture, in particular to intelligent cleaning equipment for cell culture accessories.
Background
The cell culture bag is made by waterproof ventilated membrane, and gas can pass through the microporous membrane by virtue of the diffusion principle, and liquid water or water droplet are because of its surface tension effect, and the hydrone can't pass through, and the cell culture that expands suspension cell that can be fine compares the cell culture dish, possess good gas permeability, is fit for being used for the cell culture work of large tracts of land hyperplasia.
When recovering cells on a cell culture bag, usually, nutrient solution in the cell culture bag is directly poured into a collection container, most of the cells on the cell culture bag flow out into the collection container along with the nutrient solution, chinese patent CN109321433A discloses a cell culture bag suspension drainage device, which can help laboratory staff to efficiently recover nutrient solution and cells in the cell culture bag into the collection container, but since part of the cells adhered to the inner wall of the cell culture bag cannot flow away along with fluid during the downward flow of the nutrient solution from the cell culture bag, a large amount of cells still adhere to the cell culture bag, if the cell culture bag is directly sprayed and cleaned, a large amount of adhered cells in the cell culture bag will be lost, and since the outlet of the cell culture bag is narrow, if a soaking and cleaning mode is used, the cell culture bag is filled with the nutrient solution for many times, the cells adhered to the inner wall of the cell culture bag are soaked out, a large amount of time is needed for the nutrient solution to pour out every time, a large amount of nutrient solution needs to be reused for collecting a small number of cells in the cell culture bag, the processing step of recovering the small number of cells from the nutrient solution in the later period is complicated, and the working difficulty of cleaning the inner wall of the cell culture bag is increased.
Disclosure of Invention
The invention provides intelligent cleaning equipment for cell culture accessories, aiming at overcoming the defects that when cell recovery work on a cell culture bag is carried out, a large number of cells remain on the inner wall of the cell culture bag, and the adhered cells are difficult to be effectively recovered by only spraying nutrient solution, so that the difficulty in cleaning the inner wall of the cell culture bag is high.
The technical scheme is as follows: an intelligent cleaning device for cell culture accessories comprises an inner spraying unit, a lifting unit, a cell ejecting unit, a magnetic type extrusion unit, a sleeve, a collecting pipe, a side shaft seat, a side rotating shaft, a clamping rod, a first torsion spring, a clamping block and a pressing rod; the lower side of the sleeve is communicated with a collecting pipe; the front side and the rear side of the sleeve are respectively fixedly connected with a side shaft seat; the inner sides of the two side shaft seats are respectively and rotatably connected with a side rotating shaft; two axial ends of the two side rotating shafts are fixedly connected with a clamping rod respectively; a first torsion spring is fixedly connected between the two side shaft seats and the four clamping rods respectively, and the first torsion spring is sleeved on the outer surface of the side rotating shaft; the upper ends of the four clamping rods are fixedly connected with a clamping block respectively; a pressure rod is fixedly connected between the lower ends of the left clamping rod and the right clamping rod respectively; the inner side of the sleeve is connected with an inner spraying unit for cleaning the inner wall of the cell culture bag; a lifting unit is connected between the four clamping rods; the upper end of the lifting unit is connected with a cell ejecting unit; the lower side of the cell popping unit is connected with a magnetic suction type extrusion unit; during grip block is with cell culture bag upper end centre gripping, magnetism is inhaled formula extrusion unit and is to each regional extrusion to cell culture bag in proper order, and the cell culture bag is kick-backed when leaving magnetism and inhale formula extrusion unit by the sunken region of extrusion, and the cell bullet is removed the outside that the cell culture bag is kick-backed to the unit of drive simultaneously, and the bullet removes the cell of its inner wall adhesion.
Preferably, the inner spraying unit comprises a hollow slide rod, a main conduit, a liquid inlet conduit, a double-end conduit and a movable spraying assembly; the inner side of the sleeve is connected with a hollow slide bar in a sliding way; the inner lower side of the hollow slide bar is fixedly connected with a main catheter; the lower end of the main conduit is communicated with a liquid inlet pipe; the upper side of the hollow slide bar is fixedly connected with a double-end guide pipe; the upper end of the main conduit is communicated with the double-head conduit; the upper end of the double-head guide pipe is connected with a movable spraying assembly.
Preferably, the movable spraying assembly comprises a coil pipe, a second torsion spring, a spraying pipe and a spraying head; the middle part of the upper end of the double-end guide pipe is rotatably connected with a coil pipe; a second torsion spring is fixedly connected between the front side and the rear side of the coil pipe and the double-end guide pipe respectively, and the second torsion spring is sleeved on the outer surface of the coil pipe; the left part and the right part of the coil are respectively communicated with a spraying pipe; the front side and the rear side of each spray pipe are provided with a plurality of spray heads.
Preferably, the left side of the upper end of the double-head guide pipe is provided with a slotted structure for accommodating the spray pipe.
Preferably, the plurality of spray headers are all arranged in an inclined upward structure.
Preferably, the lifting unit comprises a slide rail, an electric slide block, a mounting rack and a power transmission assembly; a slide rail is fixedly connected between the two clamping rods positioned at the rear side; a slide rail is fixedly connected between the two clamping rods positioned on the front side; the two sliding rails are respectively connected with an electric sliding block in a sliding manner; the inner sides of the two electric sliding blocks are respectively fixedly connected with a mounting rack; the two mounting frames are connected with power transmission components; the two sliding rails are connected with the power transmission assembly; the power transmission assembly and the two mounting frames are both connected with the cell ejecting unit; magnetic type extrusion unit is all connected to two mounting brackets.
Preferably, the power transmission assembly comprises a toothed plate, an external rotating shaft, a first straight gear and a second straight gear; opposite sides of the two sliding rails are fixedly connected with a toothed plate respectively; the back sides of the two mounting frames are respectively and rotatably connected with an external rotating shaft; the middle parts of the two external rotating shafts are respectively fixedly connected with a first straight gear; the two first straight gears are respectively meshed with one toothed plate; two axial ends of the two external rotating shafts are respectively fixedly connected with a second straight gear; the four second straight gears are all connected with the cell popping unit.
Preferably, the cell ejecting unit comprises an internal rotating shaft, a third straight gear, a pressing roller and a shifting block; the opposite sides of the two mounting racks are respectively and rotatably connected with an internal rotating shaft; two axial ends of the two internal rotating shafts are respectively fixedly connected with a third straight gear; the four third straight gears are respectively meshed with one second straight gear; two internal rotating shafts are respectively fixedly connected with a press roller; the outer surfaces of the two press rollers are respectively provided with a plurality of shifting blocks.
Preferably, the magnetic suction type extrusion unit comprises a fixed block, a rotating shaft, a micro motor, a limiting sliding rod, a mounting rod, an elastic piece, a magnet frame and a rubber rod; the lower sides of the two mounting frames are fixedly connected with two fixed blocks respectively; the lower sides of the four fixed blocks are respectively connected with a rotating shaft in a rotating way; the outer sides of the four fixed blocks are fixedly connected with a micro motor respectively; the output shafts of the four micro motors are fixedly connected with a rotating shaft respectively; one end of each of the four rotating shafts, which is far away from the micro motor, is fixedly connected with a limiting slide rod; one ends of the four limiting slide rods, which are far away from the fixed block, are respectively connected with an installation rod in a sliding manner; an elastic element is fixedly connected between each of the four fixed blocks and one of the mounting rods, and the elastic elements are sleeved on the limiting sliding rods; one side of each of the four mounting rods, which is far away from the fixed block, is fixedly connected with a square magnet frame; one side of each of the four mounting rods, which is far away from the fixed block, is fixedly connected with a rubber rod, and the rubber rods are positioned in the middle of the magnet frame.
Preferably, the four elastic members are insulators having a semi-cylindrical structure.
The invention has the beneficial effects that: the invention provides an intelligent cleaning device for cell culture accessories, which is characterized in that before a cell culture bag is cleaned, the cell culture bag is hung upside down and clamped in a clamping block, an outlet of the cell culture bag is communicated with a collecting pipe of a sleeve, cells in the cell culture bag flow out downwards along with nutrient solution, meanwhile, a spraying pipe extending into the sleeve sequentially sprays the nutrient solution on the inner wall of the cell culture bag from top to bottom, so that the nutrient solution in the cell culture bag flows out in a slowly descending state, a nutrient solution flowing layer is sprayed on the inner wall of the cell culture bag from top to bottom by the spraying pipe in a horizontal state, meanwhile, a magnetic suction type extrusion unit moves from top to bottom along with the spraying pipe to sequentially extrude and recess each area of the cell culture bag inwards, when the magnetic suction type extrusion unit leaves the area where the cell culture bag is extruded, the extruded area of the cell culture bag rebounds outwards, and the cell ejecting unit moving downwards along with the spray pipe is collided, so that the cells adhered to the inner wall of the cell culture bag are vibrated by the generated elasticity, the adhering area of the cells and the inner wall of the cell culture bag is reduced, the cells fall off, and the fallen cells are downwards merged into the original nutrient solution in the cell culture bag along with a nutrient solution flowing layer flowing on the inner wall of the cell culture bag and flow out of the cell culture bag;
the cell recovery device has the advantages that cells adhered to the inner wall of the cell culture bag can be efficiently recovered by using a small amount of nutrient solution, and after the inner wall of the cell culture bag is wetted by the nutrient solution flowing layer, additional cleaning treatment work is not needed when next cell culture work is carried out, so that the technical problem that the cleaning treatment difficulty of the inner wall of the cell culture bag is high due to the fact that a large amount of cells are remained on the inner wall of the cell culture bag and are difficult to effectively recover the adhered cells by spraying the nutrient solution alone in the cell recovery work is solved.
Drawings
FIG. 1 is a schematic perspective view of the intelligent cleaning apparatus for cell culture accessories;
FIG. 2 is a schematic view showing a clamping state of the intelligent cleaning device for cell culture accessories;
FIG. 3 is a partial schematic view of the clamping state of the intelligent cleaning device for cell culture accessories;
FIG. 4 is a schematic perspective view of an internal spraying unit of the intelligent cleaning device for cell culture accessories;
FIG. 5 is a schematic view of the combined three-dimensional structure of the main conduit and the double-headed conduit of the intelligent cleaning device for cell culture accessories;
FIG. 6 is a schematic view of a three-dimensional structure of a hollow slide bar of an inner spraying unit of the intelligent cleaning device for cell culture accessories;
FIG. 7 is an enlarged view of the area A1 of the inner spray unit of the intelligent cleaning device for cell culture accessories;
FIG. 8 is a schematic perspective view of the lifting unit of the intelligent cleaning device for cell culture accessories;
FIG. 9 is a schematic perspective view of a cell removal unit of the intelligent cleaning apparatus for cell culture accessories;
FIG. 10 is a schematic diagram showing a three-dimensional structure of the lifting unit and the magnetic-type pressing unit of the intelligent cleaning device for cell culture accessories;
FIG. 11 is an enlarged view of the area A2 of the magnetic attraction type pressing unit of the intelligent cleaning device for cell culture accessories.
Description of the reference numerals: 1-sleeve, 11-collecting pipe, 2-side shaft seat, 3-side rotating shaft, 4-clamping rod, 5-first torsion spring, 6-clamping block, 7-pressing rod, 8-cell culture bag, 101-hollow sliding rod, 102-main guide pipe, 1021-liquid inlet pipe, 103-double-head guide pipe, 104-coil pipe, 105-second torsion spring, 106-shower pipe, 1061-shower head, 201-sliding rail, 202-toothed plate, 203-electric sliding block, 204-mounting frame, 205-external rotating shaft, 206-first straight gear, 207-second straight gear, 301-internal rotating shaft, 302-third straight gear, 303-pressing roller, 304-shifting block, 401-fixed block, 402-rotating shaft, 403-micro motor, 404-limit slide bar, 405-installation bar, 406-elastic piece, 407-magnet frame, 408-rubber bar.
Detailed Description
The invention is further described with reference to the following figures and detailed description.
Examples
An intelligent cleaning device for cell culture accessories is shown in figures 1-11 and comprises an inner spraying unit, a lifting unit, a cell ejecting unit, a magnetic type extrusion unit, a sleeve 1, a collecting pipe 11, a side shaft seat 2, a side rotating shaft 3, a clamping rod 4, a first torsion spring 5, a clamping block 6 and a pressing rod 7; a collecting pipe 11 is communicated with the lower side of the sleeve 1; the front side and the rear side of the sleeve 1 are respectively fixedly connected with a side shaft seat 2; the inner sides of the two side shaft seats 2 are respectively connected with a side rotating shaft 3 in a rotating way; two axial ends of the two side rotating shafts 3 are respectively fixedly connected with a clamping rod 4; a first torsion spring 5 is fixedly connected between the two side shaft seats 2 and the four clamping rods 4 respectively, and the first torsion spring 5 is sleeved on the outer surface of the side rotating shaft 3; the upper ends of the four clamping rods 4 are respectively fixedly connected with a clamping block 6; a pressure lever 7 is fixedly connected between the lower ends of the two groups of left and right clamping rods 4; the inner side of the sleeve 1 is connected with an inner spraying unit; a lifting unit is connected between the four clamping rods 4; the upper end of the lifting unit is connected with a cell ejecting unit; the downside of cell removal unit is connected with magnetism and inhales formula extrusion unit.
As shown in fig. 3-7, the inner spraying unit comprises a hollow slide bar 101, a main conduit 102, a liquid inlet pipe 1021, a double-head conduit 103 and a movable spraying assembly; the inner side of the sleeve 1 is connected with a hollow slide bar 101 in a sliding way; the inner lower side of the hollow slide bar 101 is fixedly connected with a main catheter 102; the lower end of the main conduit 102 is communicated with a liquid inlet pipe 1021; a double-head guide pipe 103 is fixedly connected to the upper side of the hollow slide rod 101; the upper end of the main conduit 102 is communicated with a double-head conduit 103; the upper end of the double-head conduit 103 is connected with a movable spraying assembly.
As shown in fig. 6 and 7, the movable spray assembly comprises a coil 104, a second torsion spring 105, a spray pipe 106 and a spray header 1061; the middle part of the upper end of the double-head conduit 103 is rotatably connected with a coil 104; a second torsion spring 105 is fixedly connected between the front side and the rear side of the coil pipe 104 and the double-head guide pipe 103 respectively, and the second torsion spring 105 is sleeved on the outer surface of the coil pipe 104; the left part and the right part of the coil 104 are respectively communicated with a spray pipe 106; the front side and the rear side of each shower 106 are provided with a plurality of shower heads 1061; a slotted structure for accommodating the spray pipe 106 is formed on the left side of the upper end of the double-head guide pipe 103; the plurality of shower heads 1061 are all arranged in an upward inclined structure.
As shown in fig. 8-10, the lifting unit includes a sliding rail 201, an electric slider 203, a mounting frame 204 and a power transmission assembly; a sliding rail 201 is connected between the two clamping rods 4 on the rear side through bolts; a sliding rail 201 is also connected between the two clamping rods 4 positioned at the front side by bolts; the two sliding rails 201 are respectively connected with an electric sliding block 203 in a sliding manner; the inner sides of the two electric sliding blocks 203 are respectively connected with an installation frame 204 through bolts; the two mounting frames 204 are connected with power transmission components; the two slide rails 201 are connected with a power transmission assembly; the power transmission assembly and the two mounting frames 204 are both connected with the cell ejecting unit; both mounting brackets 204 are connected to a magnetic suction type squeezing unit.
As shown in fig. 10, the power transmission assembly includes a toothed plate 202, an outer rotating shaft 205, a first spur gear 206, and a second spur gear 207; opposite sides of the two sliding rails 201 are fixedly connected with a toothed plate 202 respectively; the opposite sides of the two mounting frames 204 are respectively connected with an external rotating shaft 205 in a rotating way; a first straight gear 206 is fixedly connected to the middle of each of the two external rotating shafts 205; each of the two first straight gears 206 is engaged with one toothed plate 202; two axial ends of the two external rotating shafts 205 are respectively fixedly connected with a second straight gear 207; the four second spur gears 207 are all connected with the cell popping unit.
As shown in fig. 9 and 10, the cell ejecting unit includes an internal rotation shaft 301, a third spur gear 302, a pressing roller 303, and a shifting block 304; the opposite sides of the two mounting frames 204 are respectively connected with an internal rotating shaft 301 in a rotating way; two axial ends of the two internal rotating shafts 301 are respectively fixedly connected with a third straight gear 302; the four third spur gears 302 are respectively meshed with one second spur gear 207; two internal rotating shafts 301 are fixedly connected with a press roller 303 respectively; a plurality of blocks 304 are provided around the outer surface of each of the two rollers 303.
As shown in fig. 10 and 11, the magnetic attraction type pressing unit includes a fixed block 401, a rotating shaft 402, a micro motor 403, a limit slide bar 404, a mounting bar 405, an elastic member 406, a magnet frame 407, and a rubber bar 408; two fixing blocks 401 are fixedly connected to the lower sides of the two mounting frames 204 respectively; the lower parts of the four fixed blocks 401 are respectively connected with a rotating shaft 402 in a rotating way; the outer sides of the four fixed blocks 401 are fixedly connected with a micro motor 403 respectively; the output shafts of the four micro motors 403 are fixedly connected with a rotating shaft 402 respectively; one end of each of the four rotating shafts 402, which is far away from the micro motor 403, is fixedly connected with a limiting slide bar 404; one ends of the four limiting sliding rods 404, which are far away from the fixed block 401, are respectively connected with a mounting rod 405 in a sliding manner; an elastic element 406 is fixedly connected between each of the four fixed blocks 401 and one mounting rod 405, and the elastic element 406 is sleeved on the limiting sliding rod 404; one sides of the four mounting rods 405 far away from the fixed block 401 are fixedly connected with a magnet frame 407; one side of each of the four mounting rods 405 away from the fixed block 401 is fixedly connected with a rubber rod 408, and the rubber rods 408 are located in the middle of the magnet frame 407.
When an experimenter uses the intelligent cleaning equipment for the cell culture accessory, the intelligent cleaning equipment is called as the intelligent cleaning equipment for short, firstly, the sleeve 1 and the clamping rods 4 on two sides are held by hands, the spray pipe 106 is twisted to drive the coil pipe 104 and the second torsion spring 105 to twist ninety degrees, the spray pipe 106 and the spray head 1061 arranged on the spray pipe 106 are rotated to be in a vertical state, the hollow slide rod 101 is pulled downwards to drive the double-head guide pipe 103 and the spray pipe 106 connected with the hollow slide rod 101 to downwards enter the sleeve 1, the spray pipe 106 is clamped in the sleeve 1 and cannot rebound along with the twisted second torsion spring 105, then the intelligent cleaning equipment is reversed by one hundred eighty degrees, the two pressure rods 7 are pressed simultaneously to drive the clamping rods 4 and the first torsion spring 5 and the clamping block 6 connected with the clamping rods 4 to be twisted outwards to be opened, the two corners of the cell culture bag 8 far away from the outlet are aligned with the four clamping rods 4, and the two pressure rods 7 are loosened, and the twisted first torsion spring 5 drives the clamping rods 4 to clamp the two cell culture bags 8 through the clamping blocks 6 to clamp the two cell culture bags 8 Corner, when rubber pole 408 is moving to cell culture bag 8 direction along with clamping bar 4, rubber pole 408 receives the blocking of nutrient solution in cell culture bag 8, and drives installation pole 405 along spacing slide bar 404 reverse movement, and elastic component 406 is compressed simultaneously, realizes by two grip blocks 6 to two corner centre gripping of cell culture bag 8 fixed simultaneously, and four elastic components 406 that are compressed push four rubber poles 408 under reverse elastic force and hug closely at the surface of cell culture bag 8.
Then an experimenter connects an outlet of the cell culture bag 8 with the sleeve 1, the collecting pipe 11 is connected with a collecting container through a pipeline, the liquid inlet pipe 1021 is connected with a nutrient solution conveyor through a pipeline, the intelligent cleaning equipment is turned over one hundred eighty degrees and returns to the right, then the hollow slide rod 101 is pushed upwards, the double-head guide pipe 103 and the spray pipe 106 are enabled to enter the cell culture bag 8 upwards along the sleeve 1, after the spray pipe 106 leaves the sleeve 1, the spray pipe 106 is driven by the twisted second torsion spring 105 to rotate ninety degrees and return to the right horizontal state, as shown in fig. 2 and fig. 3, the clamping work of the cell culture bag 8 is completed, and the spray pipe 106 is inserted into the cell culture bag 8 in the horizontal state.
After overturning the intelligent cleaning equipment by one hundred eighty degrees to drive the cell culture bag 8 to overturn, the nutrient solution in the cell culture bag 8 flows into the sleeve 1 from the outlet below the cell culture bag 8 and flows into the external collection container through the pipeline along the collection pipe 11 on the sleeve 1, and meanwhile, most cells in the cell culture bag 8 flow into the external collection container along with the flowing nutrient solution, so that the cell collection work in the nutrient solution is completed.
During the period that most cells in the cell culture bag 8 flow into the external collection container along with nutrient solution, the external nutrient solution conveyer conveys the nutrient solution into the main conduit 102 and the double-end conduit 103 through the liquid inlet pipe 1021, so that the nutrient solution enters the spray pipe 106 along the coil 104 and is sprayed to the inner wall of the cell culture bag 8 from the spray heads 1061 at the front side and the rear side of the spray pipe 106, a nutrient solution flowing layer is sprayed on the inner wall of the cell culture bag 8 from top to bottom, and the nutrient solution flowing layer is merged into the original nutrient solution in the cell culture bag 8 along the inner wall of the cell culture bag 8.
Along with the flowing out of the original nutrient solution in the cell culture bag 8, the liquid level of the original nutrient solution in the cell culture bag 8 slowly drops, meanwhile, the laboratory technician slowly pulls the hollow slide bar 101 downwards to make the spray pipe 106 always keep above the liquid level of the original nutrient solution in the cell culture bag 8 in the process of moving downwards along the hollow slide bar 101, so that a layer of nutrient solution flowing layer is always kept in the area of the inner wall of the cell culture bag 8 above the liquid level of the original nutrient solution, meanwhile, the electric slide block 203 drives the mounting frame 204 and the connected cell ejecting unit and the magnetic absorption type extrusion unit to slowly move downwards along the slide rail 201, the four rubber rods 408 in the magnetic absorption type extrusion unit are respectively clung to the front side and the back side of the cell culture bag 8 respectively by the attraction of the four magnet frames 407 which are respectively positioned at the front side and the back side and move downwards along the outer surface of the cell culture bag 8, the four rubber rods 408 sequentially press and dent each area of the cell culture bag 8 inwards during passing through each area of the cell culture bag 8 from top to bottom, when the rubber rods 408 leave the area of the cell culture bag 8 pressed downwards, the area of the cell culture bag 8 pressed rebounds outwards and collides with the pressing roller 303 in the cell removal unit moving downwards, so that the cells adhered to the inner wall of the cell culture bag 8 are vibrated by the elastic force generated by the impacting pressing roller 303, the adhering area of the cells and the inner wall of the cell culture bag 8 is reduced, the cells fall off, and the fallen cells are converged into the original nutrient solution in the cell culture bag 8 downwards along with the nutrient solution flowing layer flowing through the inner wall of the cell culture bag 8 and flow into an external collection container.
When the rubber rod 408 clings to the outer surface of the cell culture bag 8 and moves downwards to the lower end of the cell culture bag 8 along with the electric slider 203, at this time, the liquid level of the nutrient solution of the crude oil in the cell culture bag 8 drops below the outlet of the cell culture bag 8, the output shaft of the micro motor 403 drives the rotating shaft 402 and the mounting rod 405 connected with the rotating shaft to rotate, the mounting rod 405 drives the rubber rod 408 to rotate from the horizontal state to the inclined state through the elastic member 406, so that the four rubber rods 408 positioned at the front side and the rear side of the cell culture bag 8 present two splayed structures, and then the rubber rod 408 continues to move downwards along with the electric slider 203, so that when the four rubber rods 408 in the two splayed structures move along the lower side of the outer surface of the cell culture bag 8, the nutrient solution of the crude oil at the lower side of the cell culture bag 8 is pushed by the rubber rod 408 to flow out towards the outlet of the cell culture bag 8, the phenomenon that the cells in the nutrient solution are gathered at the outlet of the cell culture bag 8 and can not effectively flow away along with the nutrient solution is avoided.
In the process that the electric sliding block 203 drives the mounting frame 204 to move downwards, the mounting frame 204 drives the external rotating shaft 205 and the first spur gear 206 connected with the external rotating shaft to engage the toothed plate 202 to move and drives the second spur gear 207 to rotate, the second spur gear 207 engages the third spur gear 302 to drive the internal rotating shaft 301 and the compression roller 303 and the shifting block 304 connected with the internal rotating shaft to rotate, the downward moving compression roller 303 impacts the rebounding area of the cell culture bag 8 while the rotating shifting block 304 continuously and repeatedly impacts the rebounding area of the cell culture bag 8, the vibration amplitude of the rebounding area of the cell culture bag 8 is enhanced, the dropping effect of cells adhered to the inner wall of the cell culture bag 8 is improved, the cell recovery speed of the cells adhered to the inner wall of the cell culture bag 8 is accelerated, the cells adhered to the inner wall of the cell culture bag 8 are efficiently recovered by using a small amount of nutrient solution, and the inner wall of the cell culture bag 8 is rinsed by the nutrient solution flowing layer, when the next batch of cell culture work is carried out, no additional cleaning treatment work is needed.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by the present specification, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (6)
1. An intelligent cleaning device for cell culture accessories comprises a sleeve (1), a collecting pipe (11), a side shaft seat (2), a side rotating shaft (3), a clamping rod (4), a first torsion spring (5), a clamping block (6) and a pressing rod (7); a collecting pipe (11) is communicated with the lower side of the sleeve (1); the front side and the rear side of the sleeve (1) are respectively fixedly connected with a side shaft seat (2); the inner sides of the two side shaft seats (2) are respectively and rotatably connected with a side rotating shaft (3); two axial ends of the two side rotating shafts (3) are respectively fixedly connected with a clamping rod (4); a first torsion spring (5) is fixedly connected between the two side shaft seats (2) and the four clamping rods (4), and the first torsion spring (5) is sleeved on the outer surface of the side rotating shaft (3); the upper ends of the four clamping rods (4) are respectively fixedly connected with a clamping block (6); the lower ends of the two groups of left and right clamping rods (4) are fixedly connected with a pressure rod (7) respectively;
the device is characterized by also comprising an inner spraying unit, a lifting unit, a cell ejecting unit and a magnetic suction type extrusion unit; the inner side of the sleeve (1) is connected with an inner spraying unit for cleaning the inner wall of the cell culture bag (8); a lifting unit is connected between the four clamping rods (4); the upper end of the lifting unit is connected with a cell ejecting unit; the lower side of the cell popping unit is connected with a magnetic suction type extrusion unit; during the clamping of the upper end of the cell culture bag (8) by the clamping block (6), the magnetic suction type extrusion unit extrudes each area of the cell culture bag (8) in sequence, the area where the cell culture bag (8) is extruded and dented rebounds when leaving the magnetic suction type extrusion unit, and meanwhile, the lifting unit drives the cell removal unit to cling to the outer side of the rebounding area of the cell culture bag (8) and remove the cells adhered to the inner wall of the cell removal unit;
the inner spraying unit comprises a hollow slide bar (101), a main conduit (102), a liquid inlet conduit (1021), a double-end conduit (103) and a movable spraying assembly; the inner side of the sleeve (1) is connected with a hollow slide bar (101) in a sliding way; the inner lower side of the hollow slide bar (101) is fixedly connected with a main conduit (102); the lower end of the main conduit (102) is communicated with a liquid inlet pipe (1021); a double-head guide pipe (103) is fixedly connected to the upper side of the hollow slide rod (101); the upper end of the main conduit (102) is communicated with a double-head conduit (103); the upper end of the double-head guide pipe (103) is connected with a movable spraying assembly;
the movable spray assembly comprises a coil pipe (104), a second torsion spring (105), a spray pipe (106) and a spray head (1061); the middle part of the upper end of the double-head guide pipe (103) is rotatably connected with a coil pipe (104); a second torsion spring (105) is fixedly connected between the front side and the rear side of the coil pipe (104) and the double-head guide pipe (103), and the second torsion spring (105) is sleeved on the outer surface of the coil pipe (104); the left part and the right part of the coil (104) are respectively communicated with a spray pipe (106); the front side and the rear side of each spray pipe (106) are provided with a plurality of spray headers (1061);
the lifting unit comprises a slide rail (201), an electric slide block (203), a mounting rack (204) and a power transmission assembly; a sliding rail (201) is fixedly connected between the two clamping rods (4) positioned at the rear side; a sliding rail (201) is also fixedly connected between the two clamping rods (4) positioned at the front side; the two sliding rails (201) are respectively connected with an electric sliding block (203) in a sliding way; the inner sides of the two electric sliding blocks (203) are respectively fixedly connected with a mounting rack (204); the two mounting frames (204) are connected with power transmission components; the two sliding rails (201) are connected with a power transmission assembly; the power transmission assembly and the two mounting frames (204) are connected with the cell ejecting unit; the two mounting frames (204) are connected with the magnetic suction type extrusion unit;
the power transmission assembly comprises a toothed plate (202), an external rotating shaft (205), a first straight gear (206) and a second straight gear (207); opposite sides of the two sliding rails (201) are fixedly connected with a toothed plate (202) respectively; the opposite sides of the two mounting frames (204) are respectively and rotatably connected with an external rotating shaft (205); the middle parts of the two external rotating shafts (205) are respectively fixedly connected with a first straight gear (206); the two first straight gears (206) are respectively meshed with one toothed plate (202); two axial ends of the two external rotating shafts (205) are respectively fixedly connected with a second straight gear (207); and the four second straight gears (207) are connected with the cell ejecting unit.
2. The intelligent cleaning equipment for the cell culture accessories is characterized in that a slotted structure for accommodating the spray pipe (106) is formed on the left side of the upper end of the double-head guide pipe (103).
3. The intelligent cleaning device for the cell culture accessories, according to claim 1, wherein the plurality of spray heads (1061) are arranged in an inclined upward structure.
4. The intelligent cleaning device for the cell culture accessories is characterized in that the cell ejecting unit comprises an internal rotating shaft (301), a third spur gear (302), a pressing roller (303) and a shifting block (304); the opposite sides of the two mounting frames (204) are respectively and rotatably connected with an internal rotating shaft (301); two axial ends of the two internal rotating shafts (301) are fixedly connected with a third straight gear (302) respectively; the four third straight gears (302) are respectively meshed with one second straight gear (207); two compression rollers (303) are fixedly connected to the two internal rotating shafts (301) respectively; a plurality of shifting blocks (304) are respectively arranged around the outer surfaces of the two press rollers (303).
5. The intelligent cleaning device for the cell culture accessories is characterized in that the magnetic suction type extrusion unit comprises a fixed block (401), a rotating shaft (402), a micro motor (403), a limiting sliding rod (404), a mounting rod (405), an elastic piece (406), a magnet frame (407) and a rubber rod (408); two fixing blocks (401) are fixedly connected to the lower sides of the two mounting frames (204) respectively; the lower parts of the four fixed blocks (401) are respectively connected with a rotating shaft (402) in a rotating way; the outer sides of the four fixing blocks (401) are fixedly connected with a micro motor (403) respectively; the output shafts of the four micro motors (403) are fixedly connected with a rotating shaft (402) respectively; one ends of the four rotating shafts (402) far away from the micro motor (403) are respectively fixedly connected with a limiting sliding rod (404); one ends of the four limiting sliding rods (404) far away from the fixed block (401) are respectively connected with a mounting rod (405) in a sliding manner; an elastic element (406) is fixedly connected between each of the four fixed blocks (401) and one mounting rod (405), and the elastic element (406) is sleeved on the limiting sliding rod (404); one sides of the four mounting rods (405) far away from the fixed block (401) are fixedly connected with a square magnet frame (407) respectively; one side of each of the four mounting rods (405) far away from the fixed block (401) is fixedly connected with a rubber rod (408), and the rubber rods (408) are positioned in the middle of the magnet frame (407).
6. The intelligent cleaning device for the cell culture accessories as claimed in claim 3, wherein the four rubber rods (408) are insulators with semi-cylindrical structures.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210538927.5A CN114653702B (en) | 2022-05-18 | 2022-05-18 | Cell culture is intelligent cleaning device for accessory |
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| CN202210538927.5A CN114653702B (en) | 2022-05-18 | 2022-05-18 | Cell culture is intelligent cleaning device for accessory |
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| CN114653702B true CN114653702B (en) | 2022-08-19 |
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| US11898127B1 (en) | 2022-09-26 | 2024-02-13 | Upside Foods, Inc. | Harvesting cell-based meat |
| WO2024072444A1 (en) * | 2022-09-26 | 2024-04-04 | Upside Foods, Inc. | Harvesting cell-based meat |
| CN115382257B (en) * | 2022-10-26 | 2022-12-30 | 中基万季建设投资集团有限公司 | Sedimentation tank disinfecting equipment for sewage treatment |
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