CN114645034B - Enzyme for synthesizing high-purity diglyceride, and preparation method and application thereof - Google Patents

Enzyme for synthesizing high-purity diglyceride, and preparation method and application thereof Download PDF

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CN114645034B
CN114645034B CN202011504714.8A CN202011504714A CN114645034B CN 114645034 B CN114645034 B CN 114645034B CN 202011504714 A CN202011504714 A CN 202011504714A CN 114645034 B CN114645034 B CN 114645034B
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mrl
lipase
diglyceride
enzyme
gene
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CN114645034A (en
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昝新艺
陈志蔚
陈宇星
崔凤杰
叶萍萍
霍书豪
何文森
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Jiangsu Hefeng Grain And Oil Industry Co ltd
Jiangsu University
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Jiangsu University
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/70—Vectors or expression systems specially adapted for E. coli
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6454—Glycerides by esterification

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Abstract

The invention belongs to the fields of food biotechnology and oil processing and comprehensive utilization, and particularly relates to an enzyme for synthesizing diglyceride, a preparation method and application thereof; s2: construction of recombinant plasmids: the gene sequence of Malassezia restricta lipase MRL obtained in the step S1 is used as a template, the lipase MRL gene is amplified by using an upstream primer F and a downstream primer R, and is connected with a plasmid pET30a after double enzyme digestion, so that a recombinant plasmid pET30a-MRL is obtained. The marine bacterium Malassezia restricta lipase MRL prepared by the invention has good esterification activity, can efficiently catalyze glycerol and fatty acid to form monoglyceride and diglyceride, and the content of the diglyceride in the product reaches 30% -50%. The high-purity grease obtained by molecular distillation of the product contains 80-93% of diglyceride, and has obvious industrial production potential.

Description

Enzyme for synthesizing high-purity diglyceride, and preparation method and application thereof
Technical Field
The invention belongs to the fields of food biotechnology and oil processing and comprehensive utilization, and in particular relates to an enzyme for synthesizing diglyceride, a preparation method and application thereof.
Background
Diglyceride (DAG) is a structural lipid formed by esterifying two hydroxyl groups in glycerol with fatty acid, mainly comprising two isomers of 1,2-DAG and 1,3-DAG, and is a well-recognized safety (GRAS) food ingredient. The metabolic pathway of diglyceride is different from that of triglyceride, and after being taken by human body, it is decomposed by pancreatic lipase to produce glycerin and free fatty acid, and can be directly converted into energy, and does not participate in blood lipid synthesis, so that it can not raise blood lipid, and can not cause obesity. Therefore, the diglyceride can be used as a health care grease and a medical intermediate, and has very important application value in the industries of food, medicine and chemical industry.
Diglycerides are products with different DAG contents, which are prepared by taking animal and vegetable oil, glycerol and fatty acid as raw materials through hydrolysis, esterification, transesterification, acidolysis, alcoholysis and other reactions. In general, there are three methods for preparing diglycerides: hydrolysis, glycerolysis and esterification. The hydrolysis method is to moderately hydrolyze refined animal and vegetable oil with sn-1, 3-site specific lipase to obtain diglyceride, and the DAG content of the oil prepared by the method is usually not more than 60% after molecular distillation purification. The glycerolysis method adopts free enzyme or immobilized enzyme, uses refined animal and vegetable oil and glycerin as substrates, and carries out glycerolysis reaction under a solvent or solvent-free system, wherein the DAG content in the oil prepared by the method is generally 50-85%, but the reaction is greatly restricted by the type of enzyme preparation, the solvent, the lipase position selectivity and other conditions. The esterification method is to esterify glycerin and fatty acid by using lipase or partial glyceride lipase with sn-1, 3-position specificity to obtain the DAG-rich grease, the DAG content of the prepared DAG is up to more than 90%, especially the DAG content of the grease prepared by esterification of partial glyceride lipase can be up to more than 97%. Therefore, the esterification method for producing high-purity diglycerides has great advantages.
At present, the partial glyceride lipase for producing high-purity diglyceride has few sources, the commercial partial glyceride lipase with sn-1, 3-position specificity is respectively from thermophilic fungi Thermomyces lanuginosus and penicillium Penicillium camemberti, and is a powdery free enzyme preparation obtained by fermentation production, purification and drying, and the cost is high. The only partial glyceride lipases reported in the literature include lipases SMG1, mgDL2 and PCL, but none of them is used for commercial production of diglycerides.
Therefore, the invention provides an enzyme for synthesizing high-purity diglyceride, and a preparation method and application thereof.
Disclosure of Invention
The invention aims at solving the defect of the prior art for preparing diglyceride lipase resources in China, providing an enzyme for synthesizing high-purity diglyceride, a preparation method and application thereof, determining the optimal condition for synthesizing diglyceride, and enriching the lipase resources for producing diglyceride by an esterification method in China.
In order to achieve the above purpose, the present invention adopts the following technical scheme.
A preparation method of an enzyme for synthesizing high-purity diglyceride comprises the following steps:
s1: by synthesizing the gene of marine bacterium Malassezia restricta lipase MRL;
s2: construction of recombinant plasmids: the gene sequence of Malassezia restricta lipase MRL obtained in the step S1 is used as a template, the lipase MRL gene is amplified by using an upstream primer F and a downstream primer R, and is connected with a plasmid pET30a after double enzyme digestion, so that a recombinant plasmid pET30a-MRL is obtained.
S3: construction of lipase MRL protein expression strains: the recombinant plasmid pET30a-MRL is introduced into escherichia coli BL21, and positive transformants are obtained through screening, namely the lipase MRL protein expression strain.
S4: lipase MRL protein expression and purification: culturing lipase MRL protein expression strain, inducing protein expression, purifying and drying to obtain lipase MRL dry powder.
Preferably, when the molar ratio of oleic acid to glycerol is 1:1-1:5, the enzyme addition amount is 2% -10% of the total mass of the substrate, the reaction temperature is 25 ℃ -50 ℃, the content of diglyceride in the obtained oil after 2-20 h reaction is 30% -50%, and the esterification rate can reach 60% -85%.
The invention has the following beneficial effects:
the partial glyceride lipase MRL from marine bacteria Malassezia restricta prepared by the invention has good esterification activity, can efficiently catalyze glycerin and fatty acid to form monoglyceride and diglyceride, the esterification rate reaches 60% -85%, the diglyceride content in the product reaches 30% -50%, and the high-purity grease obtained by molecular distillation of the product contains 80-93% of diglyceride, so that the partial glyceride lipase MRL has obvious industrial production potential.
Drawings
FIG. 1 is a schematic diagram of construction of lipase MRL gene expression plasmid.
FIG. 2 is an electrophoresis diagram of the purification of the MRL protein of the lipase.
FIG. 3 shows the effect of the addition of lipase MRL on the esterification rate and glyceride content.
In the figure: m is a protein Marker; 1. is whole cell lysate; 2. is supernatant protein; 3. is a cell debris; 4. the protein purified by the Ni column; 5. is desalted protein.
Detailed Description
The following description of the technical solutions in the embodiments of the present invention will be clear and complete, and it is obvious that the described embodiments are only some embodiments of the present invention, but not all embodiments.
A preparation method of an enzyme for synthesizing high-purity diglyceride comprises the following steps:
s1: by synthesizing the gene of marine bacterium Malassezia restricta lipase MRL;
s2: construction of recombinant plasmids: the gene sequence of Malassezia restricta lipase MRL obtained in the step S1 is used as a template, the lipase MRL gene is amplified by using an upstream primer F and a downstream primer R, and is connected with a plasmid pET30a after double enzyme digestion, so that a recombinant plasmid pET30a-MRL is obtained.
S3: construction of lipase MRL protein expression strains: the recombinant plasmid pET30a-MRL is introduced into escherichia coli BL21, and positive transformants are obtained through screening, namely the lipase MRL protein expression strain.
S4: lipase MRL protein expression and purification: culturing lipase MRL protein expression strain, inducing protein expression, purifying and drying to obtain lipase MRL dry powder.
(1) Strains, enzymes and culture media
Chemically competent cells of E.coli Escherichia coli Top and E.coli Escherichia coli BL; DNA polymerase (Ex Taq DNA polymerase) was purchased from Dalian Takara, DNA ligase (T4 DNA ligase) and restriction endonucleases NdeI and HindIII were purchased from Thermo Fisher Scientific; the LB medium comprises the following components of Tryptone 10 g/L, yeast extract 5 g/L and NaCl 10 g/L; kanamycin concentration was 50 ug/mL; expression vector pET30a was stored for this laboratory.
(2) Gene synthesis, PCR amplification and recombinant plasmid construction of lipase MRL
In this example, blast alignment analysis was performed in NCBI database based on the disclosed amino acid sequence of partial glyceride lipase SMG1 (Genbank ID: EDP 44990.1), and gene synthesis was performed to obtain the gene sequence (SEQ ID NO. 1) of marine bacterium Malassezia restricta lipase MRL having extremely high sequence homology with it.
The gene sequence of the synthesized lipase MRL is used as a template, and the gene of the lipase MRL is amplified by PCR by using an upstream primer F (the sequence is shown as SEQ ID NO. 3) and a downstream primer R (the sequence is shown as SEQ ID NO. 4). The system of PCR reaction (50) uL was as follows: template 1 uL (50 ng), dNTP mix 1 uL, primers (10 uM/L) each 1 uL,10 XPCR buffer 5 uL, DNA polymerase (5U/L) 0.2 uL, and sterile water to 50 uL. The PCR amplification conditions were as follows: 94. 3 min at 94 ℃, 30 s at 56 ℃, 30 s at 72 ℃ for 1 min,31 cycles. The PCR amplified product was recovered by agarose electrophoresis, digested with restriction enzymes NdeI and HindIII, ligated with plasmid pET30a digested with NdeI and HindIII, transformed into E.coli Top10, and positive transformants were selected, cultured overnight at 37℃and 200rpm, and plasmids were extracted to obtain recombinant plasmid pET30a-MRL (plasmid map see FIG. 1).
(3) Construction, induced expression and purification of lipase MRL gene expression strain
And (3) introducing the recombinant plasmid pET30a-MRL into the chemocompetence of the escherichia coli BL21 by adopting a heat shock method, and obtaining a positive transformant by colony PCR verification, namely the lipase MRL protein expression strain. E.coli BL21 introduced with recombinant plasmid pET30a-MRL is inoculated into LB culture medium containing 50 ug/mL kanamycin, cultured at 37 ℃ and 200rpm until the OD value reaches 0.6-0.8, added with IPTG with the final concentration of 0.5 mM to induce 16 h at 15 ℃, centrifugally collected thalli, added with lysate (20 mM Tris-HCl, 500 mM NaCl, 5% glycol and pH 8.0) to carry out ultrasonic disruption, centrifuged at 4 ℃ and 15000 rpm for 20 min, and the supernatant is taken out, and then separated and purified by a Ni-NTA purification resin pre-packed column of 5 mL.
The Binding buffer solution required by Ni-NTA purification resin pre-packed column separation and purification of lipase MRL comprises the following components: 20 mM Tris-HCl, 500 mM NaCl, 10 mM imidazole, pH 8.0; the composition of the solution buffer is as follows: 20 M Tris-HCl, 500 mM NaCl, 250 mM imidazole, pH 8.0; the elution rate was 1 mL/min. The protein samples obtained by column chromatography were subjected to SDS-PAGE gel electrophoresis to examine the purification effect (as shown in FIG. 2). And desalting and freeze-drying the purified sample to obtain lipase MRL dry powder.
Example 2
A method for preparing an enzyme for synthesizing high-purity diglyceride, which is characterized by comprising the following steps:
s1: by synthesizing the gene of marine bacterium Malassezia restricta lipase MRL;
s2: construction of recombinant plasmids: the gene sequence of Malassezia restricta lipase MRL obtained in the step S1 is used as a template, the lipase MRL gene is amplified by using an upstream primer F and a downstream primer R, and is connected with a plasmid pET30a after double enzyme digestion, so that a recombinant plasmid pET30a-MRL is obtained.
S3: construction of lipase MRL protein expression strains: the recombinant plasmid pET30a-MRL is introduced into escherichia coli BL21, and positive transformants are obtained through screening, namely the lipase MRL protein expression strain.
S4: lipase MRL protein expression and purification: culturing lipase MRL protein expression strain, inducing protein expression, purifying and drying to obtain lipase MRL dry powder.
Analysis of Effect of Lipase MRL on hydrolysis of different glyceride substrates
10 g camellia oil (triglyceride content 98%), diglycerides (1, 2-and 1, 3-dioleate, available from Avanti) and monoglyceride were added to 3 50 mL triangular flasks, respectively, and 5 g water was added thereto, followed by preheating in a shaker at 30℃for 20 min, and then adding 100U/g (U/w, mass of oil) of lipase MRL, for reaction. Samples were taken at intervals of 150 uL, centrifuged at 12000 rpm for 2 min, the upper oil phase was taken in a 1.5 mLEP tube and the glyceride composition of the samples was checked by HPLC. The mobile phase of HPLC was: n-hexane/isopropanol/formic acid (15:1:0.003, v/v/v), filtration with a 0.45. 0.45 uM filter membrane, and degassing by sonication for 30 min; the column was a silica column (250 mm X4.6 mm, particle size 5 μm). 20 uL oil sample was taken and mixed with 1 mL mobile phase, dehydrated with anhydrous sodium sulfate and transferred to a sample bottle. The content of triglycerides, diglycerides, monoglycerides and free fatty acids in the samples was determined by HPLC. As a result, it was found that lipase MRL was not capable of hydrolyzing camellia oil (triglyceride content 98%), was capable of hydrolyzing diglycerides (1, 3-DAG,1, 2-DAG) and monoglycerides, and it was confirmed that lipase MRL was a monoester and diester lipase, belonging to the partial glyceride lipase.
Example 3
A method for preparing an enzyme for synthesizing high-purity diglyceride, which is characterized by comprising the following steps:
s1: genes for lipase MRL by marine bacteria Malassezia restricta;
s2: construction of recombinant plasmids: the gene sequence of Malassezia restricta lipase MRL obtained in the step S1 is used as a template, the lipase MRL gene is amplified by using an upstream primer F and a downstream primer R, and is connected with a plasmid pET30a after double enzyme digestion, so that a recombinant plasmid pET30a-MRL is obtained.
S3: construction of lipase MRL protein expression strains: the recombinant plasmid pET30a-MRL is introduced into escherichia coli BL21, and positive transformants are obtained through screening, namely the lipase MRL protein expression strain.
S4: lipase MRL protein expression and purification: culturing lipase MRL protein expression strain, inducing protein expression, purifying and drying to obtain lipase MRL dry powder.
Hydrolysis Activity determination of Lipase MRL
The activity of lipase MRL was determined by diglyceride emulsification: first, 4% of polyvinyl alcohol AH-26 was mixed with diglyceride (1, 3-dioleate, available from Avanti) at a mass ratio of 3:1, and homogeneously emulsified with a homogenizer at 10 000 rpm for 10 min until the emulsion became milky white and free of oil droplets. Then, adding diglyceride emulsion 4 mL and 5 mL of KH2PO4-Na2HPO4 buffer of 0.05 mol/L with pH 5.6 into a triangular flask, preheating for 5 min in a constant temperature magnetic stirrer at 30 ℃, accurately adding enzyme solution 100 uL and starting timing, and immediately adding 95% ethanol 15 mL after 30 min of reaction to terminate the reaction. Finally, the reaction flask is taken out, 1 drop of phenolphthalein indicator is added, the calibrated KOH solution with the concentration of about 0.05 mol/L is used for titrating free fatty acid released by the hydrolysis of the lipase MRL, and the volume consumed by KOH is accurately recorded. The blank test is to add 95% ethanol into the preheated reaction system, then add the enzyme solution 100 uL (the enzyme solution is heated for 15 min at 100 ℃ and used after being cooled to room temperature) after heat inactivation treatment, and then react for 30 min, and the other operations are the same as before. 1 enzyme activity unit (U): under the above assay conditions (i.e., specific temperature and reaction conditions), the amount of enzyme required to hydrolyze the diglyceride per minute to produce 1 umol of titratable fatty acid. The lipase MRL has a hydrolase activity of 4800U/g as measured by the above method using diglyceride as a substrate.
Example 4
A method for preparing an enzyme for synthesizing high-purity diglyceride, which is characterized by comprising the following steps:
s1: genes for lipase MRL by marine bacteria Malassezia restricta;
s2: construction of recombinant plasmids: the gene sequence of Malassezia restricta lipase MRL obtained in the step S1 is used as a template, the lipase MRL gene is amplified by using an upstream primer F and a downstream primer R, and is connected with a plasmid pET30a after double enzyme digestion, so that a recombinant plasmid pET30a-MRL is obtained.
S3: construction of lipase MRL protein expression strains: the recombinant plasmid pET30a-MRL is introduced into escherichia coli BL21, and positive transformants are obtained through screening, namely the lipase MRL protein expression strain.
S4: lipase MRL protein expression and purification: culturing lipase MRL protein expression strain, inducing protein expression, purifying and drying to obtain lipase MRL dry powder.
The oleic acid and the glycerol are used as substrates, the influence of enzyme addition amount, reaction substrate molar ratio and reaction temperature on the synthesis of diglyceride by the esterification of the glycerol and the fatty acid by the lipase MRL is researched through a single factor experiment, and the optimal reaction condition for synthesizing the oleic acid diglyceride is determined.
(1) Influence of the enzyme addition amount on the esterification reaction
Mixing oleic acid and glycerin according to a molar ratio of 1:4, adding into a triangular flask, setting the addition amount of lipase MRL to be 2, 4, 6, 8 and 10% of the total mass of a substrate, and placing the triangular flask at 30 ℃ for stirring reaction. The upper layer oil was sampled at timing (2, 4, 6, 8, 10, 20, h), the glyceride composition of the mixture was analyzed by the HPLC method in example 2, and the esterification rate was calculated. The effect of the amount of lipase MRL addition on the esterification rate and glyceride content is shown in FIG. 3.
(2) Effect of reaction substrate molar ratio on esterification reaction
Mixing oleic acid and glycerol according to a certain molar ratio (1:1, 1:2, 1:3, 1:4 and 1:5), adding into a triangular flask, wherein the addition amount of lipase MRL is 6% of the total mass of a substrate, and placing the triangular flask at 30 ℃ for stirring reaction. The upper layer oil was sampled at timing (2, 4, 6, 8, 10, 20, h), the glyceride composition of the mixture was analyzed by the HPLC method in example 2, and the esterification rate was calculated.
(3) Influence of the reaction temperature on the esterification reaction
Mixing oleic acid and glycerol according to a molar ratio of 1:4, adding into a triangular flask, wherein the addition amount of lipase MRL is 6% of the total mass of the substrate, and respectively placing the triangular flask at 25 ℃, 30 ℃, 35 ℃, 40 ℃ and 50 ℃ for stirring reaction. The upper layer oil was sampled at timing (2, 4, 6, 8, 10, 20, h), the glyceride composition of the mixture was analyzed by the HPLC method in example 2, and the esterification rate was calculated.
The result shows that when the molar ratio of oleic acid to glycerin is 1:4, the enzyme addition amount is 6% of the total mass of the substrate, the reaction temperature is 30 ℃, the reaction is 20. 20h, the content of diglyceride is 49.61%, and the esterification rate can reach 84.31%.
SEQ ID NO. 1: gene sequence of marine bacterium Malassezia restricta lipase MRL
ATGCTCTTCAATCGTATTGCTCTGTTTGCTGCAAGCTGCGCGGCTCTTGTATCTGCCGGTCCATTGAACTTTCATGCCGGTAGTTCGAAGGATCAGCCAGTCTCCAACAACTGGAACACTAAGGAAATCTCGCAGGCCGCAGGTCTTGTTCAACAGACCTACTGTGACCGTTCCACTACTAAGCCTGGTCTGAAGATCGGAGATTCTACTCTTCTTTTCACTGCTGGTAATGGTGATCATCGCCAGCGCTTCAATCTGTACCACTCGGAGAGCCTTGGAATTGCCGTGGCCATTGAAGGTACAAACCTGACCTCGATTACGTCTGATCTCCATGATGTCAAGGGTCTACCTGTCTTGCCTCATCCGCGCTACCGCTCGCATTATCCTGAGGGCACAAGGGTGATGCATGGCTTCCAGGAGGCCTACGTCAGTATCATGGACGATATGGTACGCGCTATCGAAAAGTACAAGAAGGAAAAGAATGAGAAACGAGTCACCATAATTGGTCACTCGCTGGGTGCTGCTATGGGTTTGCTTGCTGCTATGGATGTTGAACTTCGCCTGGATGACGGTATTTGCAAGACTTATCTATTTGGTCTGCCCCGCGTCGGTAACCCAACCTTTGCTGGATTCGTTGACGAAAAGATCGGTCATAAGTTTTACTCAATCATCAATGGTCAAGACTGGGTCCCCGCCGTCCCTCCTCGTGCCCTTGGTTATCAGCACCCATCGAACTACTTGTGGATTTACCCAGGTAACTCGACTAATGCTAAGATCTATCCCGGCCAGGAAAACGTCCACGGTATTCTTACTGTGCCCCGCGCCCCTAACTTCTACGACCACCAGGGCGTGTACTTCCACACTCAAATCGGTGCTTCCTTCGGTCAGTGCCCTGCCAAGGTTGGCAACATGTAA
SEQ ID NO. 2: amino acid sequence of marine bacterium Malassezia restricta lipase MRL
MLFNRIALFAASCAALVSAGPLNFHAGSSKDQPVSNNWNTKEISQAAGLVQQTYCDRSTTKPGLKIGDSTLLFTAGNGDHRQRFNLYHSESLGIAVAIEGTNLTSITSDLHDVKGLPVLPHPRYRSHYPEGTRVMHGFQEAYVSIMDDMVRAIEKYKKEKNEKRVTIIGHSLGAAMGLLAAMDVELRLDDGICKTYLFGLPRVGNPTFAGFVDEKIGHKFYSIINGQDWVPAVPPRALGYQHPSNYLWIYPGNSTNAKIYPGQENVHGILTVPRAPNFYDHQGVYFHTQIGASFGQCPAKVGNM
SEQ ID NO. 3: upstream primer F sequence
CCATATGATGCTCTTCAATCGTATTGCTC
SEQ ID NO. 4: downstream primer F sequence
CAAGCTTCATGTTGCCAACCTTGGC
The foregoing is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art, who is within the scope of the present invention, should make equivalent substitutions or modifications according to the technical scheme of the present invention and the inventive concept thereof, and should be covered by the scope of the present invention.
<110> Jiangsu Hefeng grain and oil industry Co., ltd., jiangsu university
<120> an enzyme for synthesizing high purity diglyceride, its preparation method and application
<160> 2
<170> PatentIn version 3.5
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atgctcttca atcgtattgc tctgtttgct gcaagctgcg cggctcttgt atctgccggt 60
ccattgaact ttcatgccgg tagttcgaag gatcagccag tctccaacaa ctggaacact 120
aaggaaatct cgcaggccgc aggtcttgtt caacagacct actgtgaccg ttccactact 180
aagcctggtc tgaagatcgg agattctact cttcttttca ctgctggtaa tggtgatcat 240
cgccagcgct tcaatctgta ccactcggag agccttggaa ttgccgtggc cattgaaggt 300
acaaacctga cctcgattac gtctgatctc catgatgtca agggtctacc tgtcttgcct 360
catccgcgct accgctcgca ttatcctgag ggcacaaggg tgatgcatgg cttccaggag 420
gcctacgtca gtatcatgga cgatatggta cgcgctatcg aaaagtacaa gaaggaaaag 480
aatgagaaac gagtcaccat aattggtcac tcgctgggtg ctgctatggg tttgcttgct 540
gctatggatg ttgaacttcg cctggatgac ggtatttgca agacttatct atttggtctg 600
ccccgcgtcg gtaacccaac ctttgctgga ttcgttgacg aaaagatcgg tcataagttt 660
tactcaatca tcaatggtca agactgggtc cccgccgtcc ctcctcgtgc ccttggttat 720
cagcacccat cgaactactt gtggatttac ccaggtaact cgactaatgc taagatctat 780
cccggccagg aaaacgtcca cggtattctt actgtgcccc gcgcccctaa cttctacgac 840
caccagggcg tgtacttcca cactcaaatc ggtgcttcct tcggtcagtg ccctgccaag 900
gttggcaaca tgtaa 915
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Met Leu Phe Asn Arg Ile Ala Leu Phe Ala Ala Ser Cys Ala Ala Leu
1 5 10 15
Val Ser Ala Gly Pro Leu Asn Phe His Ala Gly Ser Ser Lys Asp Gln
20 25 30
Pro Val Ser Asn Asn Trp Asn Thr Lys Glu Ile Ser Gln Ala Ala Gly
35 40 45
Leu Val Gln Gln Thr Tyr Cys Asp Arg Ser Thr Thr Lys Pro Gly Leu
50 55 60
Lys Ile Gly Asp Ser Thr Leu Leu Phe Thr Ala Gly Asn Gly Asp His
65 70 75 80
Arg Gln Arg Phe Asn Leu Tyr His Ser Glu Ser Leu Gly Ile Ala Val
85 90 95
Ala Ile Glu Gly Thr Asn Leu Thr Ser Ile Thr Ser Asp Leu His Asp
100 105 110
Val Lys Gly Leu Pro Val Leu Pro His Pro Arg Tyr Arg Ser His Tyr
115 120 125
Pro Glu Gly Thr Arg Val Met His Gly Phe Gln Glu Ala Tyr Val Ser
130 135 140
Ile Met Asp Asp Met Val Arg Ala Ile Glu Lys Tyr Lys Lys Glu Lys
145 150 155 160
Asn Glu Lys Arg Val Thr Ile Ile Gly His Ser Leu Gly Ala Ala Met
165 170 175
Gly Leu Leu Ala Ala Met Asp Val Glu Leu Arg Leu Asp Asp Gly Ile
180 185 190
Cys Lys Thr Tyr Leu Phe Gly Leu Pro Arg Val Gly Asn Pro Thr Phe
195 200 205
Ala Gly Phe Val Asp Glu Lys Ile Gly His Lys Phe Tyr Ser Ile Ile
210 215 220
Asn Gly Gln Asp Trp Val Pro Ala Val Pro Pro Arg Ala Leu Gly Tyr
225 230 235 240
Gln His Pro Ser Asn Tyr Leu Trp Ile Tyr Pro Gly Asn Ser Thr Asn
245 250 255
Ala Lys Ile Tyr Pro Gly Gln Glu Asn Val His Gly Ile Leu Thr Val
260 265 270
Pro Arg Ala Pro Asn Phe Tyr Asp His Gln Gly Val Tyr Phe His Thr
275 280 285
Gln Ile Gly Ala Ser Phe Gly Gln Cys Pro Ala Lys Val Gly Asn Met
290 295 300
<210> 3
<211> 29
<212> DNA
<213> unknown
<400> 3
ccatatgatg ctcttcaatc gtattgctc 29
<210> 4
<211> 25
<212> DNA
<213> unknown
<400> 4
caagcttcat gttgccaacc ttggc 25

Claims (1)

1. The application of the enzyme for synthesizing the high-purity diglyceride is characterized in that when the molar ratio of oleic acid to glycerol is 1:4, marine bacterium Malassezia restricta lipase MRL with a gene sequence shown as SEQ ID NO.1 is added as 6% of the total mass of the substrate, the reaction temperature is 30 ℃, the content of the diglyceride in the oil obtained after 20h of reaction is 49.61%, and the esterification rate can reach 84.31%.
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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102676592A (en) * 2012-02-29 2012-09-19 华南理工大学 Application of lipase SMG1 in preparation of fatty acid monoglyceride
CN103525784A (en) * 2013-09-30 2014-01-22 华南理工大学 Partial glyceride lipase mutant, plasmids, recombination strains, preparation methods and applications
CN103966187A (en) * 2014-04-30 2014-08-06 华南理工大学 Low-temperature partial glyceride lipase of marine microorganism source and application thereof
CN110777170A (en) * 2019-11-06 2020-02-11 华南理工大学 Method for synthesizing diglyceride
CN110951796A (en) * 2019-12-31 2020-04-03 华南理工大学 Method for converting fatty acid ethyl ester into diglyceride

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Publication number Priority date Publication date Assignee Title
US20120276247A1 (en) * 2011-04-29 2012-11-01 Uwe Bornscheuer Lipase Variants

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102676592A (en) * 2012-02-29 2012-09-19 华南理工大学 Application of lipase SMG1 in preparation of fatty acid monoglyceride
CN103525784A (en) * 2013-09-30 2014-01-22 华南理工大学 Partial glyceride lipase mutant, plasmids, recombination strains, preparation methods and applications
CN103966187A (en) * 2014-04-30 2014-08-06 华南理工大学 Low-temperature partial glyceride lipase of marine microorganism source and application thereof
CN110777170A (en) * 2019-11-06 2020-02-11 华南理工大学 Method for synthesizing diglyceride
CN110951796A (en) * 2019-12-31 2020-04-03 华南理工大学 Method for converting fatty acid ethyl ester into diglyceride

Non-Patent Citations (5)

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Title
Isolation and Expression of a Malassezia globosa Lipase Gene, LIP1;Yvonne M.DeAngelis;Journal of Investigative Dermatology;2138-2146 *
lipase [Malassezia restricta] Accession.No.XP_027482916.1;PARK.M;Genbank Database;第1-2页 *
卷枝毛霉脂肪酶及其调控脂质代谢的机制;昝新艺;中国博士学位论文全文数据库（电子期刊）工程科技Ⅰ辑;B018-15 *
基于序列结构分析的新型甘油二酯脂肪酶挖掘策略研究;SHAHID ALI;中国博士学位论文全文数据库（电子期刊）工程科技Ⅰ辑;B016-33 *
马拉色菌脂肪酶SMG1酶学性质表征及晶体学研究;刘璐;中国博士学位论文全文数据库（电子期刊）工程科技Ⅰ辑;B014-67 *

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