CN114642762A

CN114642762A - Protein-removing and sterilizing device and method for hard corneal contact lens

Info

Publication number: CN114642762A
Application number: CN202011505217.XA
Authority: CN
Inventors: 孙碧霞
Original assignee: Suzhou 3n Biological Technology Co ltd
Current assignee: Suzhou 3n Biological Technology Co ltd
Priority date: 2020-12-18
Filing date: 2020-12-18
Publication date: 2022-06-21

Abstract

The invention discloses a protein removing and sterilizing device and a method thereof for a hard corneal contact lens, wherein the protein removing and sterilizing device comprises a body, an electricity storage device, a circuit board and a switch assembly are accommodated in the body, the electricity storage device and the switch assembly are respectively connected with the circuit board, and a cleaning water tank is arranged on one side of the body; the electrophoresis dissociation chamber is arranged at one side of the body provided with the cleaning water tank, at least one group of electrophoresis dissociation probes is arranged in the electrophoresis dissociation chamber, and the electrophoresis dissociation chamber is filled with Cl^‑The electrophoresis dissociation probe is contacted with the electrolyte solution and is electrically connected with the circuit board; and the liquid containing groove is arranged on the other side of the body, and the position of the liquid containing groove corresponds to the position of the cleaning water tank. The application can be used for tearing eggsDirty thing such as white separates from rigid corneal contact lens, can also decompose tear protein, and the disinfection of can also disinfecting simultaneously, and the deproteinization sterilization apparatus that removes of this application still is equipped with washing basin and flourishing cistern in addition, still washes rigid corneal contact lens with convenient after the washing in the electrophoresis dissociation storehouse.

Description

Protein-removing and sterilizing device and method for hard corneal contact lens

Technical Field

The invention relates to the technical field of corneal contact lens cleaning, in particular to a rigid corneal contact lens protein removal and sterilization device and a rigid corneal contact lens protein removal and sterilization method.

Background

The problem of how to remove protein from contact lenses troubles the industry for more than half a century, and causes high importance of contact lens wearing safety in the eye vision industry of various countries. Because the cases of corneal infection on corneal contact lenses are frequent in China, contact lenses are listed as a third class of medical instruments in 2012 and are used as high risk management, and the reasons are as follows: the contact lens material structure has a large number of fiber itching-penetrating holes invisible to naked eyes, a large number of tears are secreted by human eyes at any time, a large number of lacrimal proteins are contained in the tears, and the lacrimal proteins easily permeate into the fiber itching-penetrating holes to cause the DK value of the lens to be reduced (oxygen permeability), corneal anoxia, edema and other symptoms, and serious problems of corneal damage, bacterial infection, corneal inflammation, even vision damage and the like can be caused.

In order to remove bacteria on the surface of the contact lens, the lens is physically rubbed by matching with a care solution or soaked by using a chemical active agent to achieve the purpose of removing protein in the traditional cleaning method, but the protein removing effect of the means is very little no matter the experimental data at home and abroad or the feedback of a user, and the scratching and the damage of the lens are easily caused by rubbing with fingers, especially hard corneal contact lenses.

Electrophoresis (EP) is a short term for electrophoresis, and refers to a phenomenon in which charged particles move toward an electrode opposite in electrical property to the charged particles under the action of an electric field. A technique of achieving separation by utilizing a difference in moving speed of charged particles in an electric field is called an electrophoresis technique. Protein Electrophoresis (SPE) is an analytical technique for proteins, which are negatively or positively charged in a buffer solution and move to the anode or cathode in an electric field, and is called electrophoresis, and different protein molecules have different electrophoretic mobilities. Electrolysis (Electrolysis) is a process in which an electric current is passed through an electrolyte solution or a molten electrolyte (also called an electrolyte) to cause redox reactions at a cathode and an anode, and an electrochemical cell can perform an Electrolysis process when a direct current voltage is applied. Typically, the electrolyte solution is not suitable for direct contact with the eye.

Therefore, in combination with the above-mentioned technical problems, a new technical solution is needed.

Disclosure of Invention

The invention aims to solve the problems in the prior art and provides a deproteinizing and sterilizing device and a method thereof suitable for a hard corneal contact lens, which can decompose tear protein and sterilize and disinfect while separating dirty matters such as tear protein from the hard corneal contact lens by an electrophoretic dissociation technology, and simultaneously, a cleaning water tank and a liquid containing tank are arranged on the deproteinizing and sterilizing device, so that the hard corneal contact lens can be conveniently and quickly washed after being cleaned in an electrophoretic dissociation bin, and the specific scheme is as follows:

according to one aspect of the present invention, the present invention provides a rigid contact lens deproteinizing and sterilizing apparatus, comprising: the cleaning device comprises a body, a cleaning device and a cleaning device, wherein an accommodating cavity is formed in the body, an electricity storage device, a circuit board and a switch assembly are accommodated in the accommodating cavity, the electricity storage device and the switch assembly are respectively and electrically connected with the circuit board, and a cleaning water tank is arranged on one side of the body; the electrophoresis separates the storehouse, the electrophoresis separates the storehouse and locates the body is equipped with the one side of wasing the basin, at least, be provided with a set of electrophoresis in the electrophoresis separates the storehouse and separate the probe, the electrophoresis separates the storehouse intussuseption and is filled with and contains Cl^-The electrophoretic dissociation probe and the Cl-containing compound^-The electrophoretic dissociation probe is electrically connected with the circuit board; and the liquid containing groove is arranged on the other side of the body, and the position of the liquid containing groove corresponds to that of the washing water tank.

Furthermore, the washing basin is the fretwork design, the opening orientation in flourishing cistern the washing basin, the washing basin can be convenient for hard cornea contact lens is in remove the washing after the albumen sterilization in the electrophoresis dissociation storehouse, and the washing liquid after washing can pass through the hole inflow in the washing basin is to flourishing cistern.

Furthermore, the body includes body upper cover and body base, the flourishing cistern is located one side of body base, the flourishing cistern with body base is the components of a whole that can function independently structure, the body upper cover block is in on body base and the flourishing cistern, the washing basin is located one side that the body upper cover kept away from the flourishing cistern, the flourishing cistern can be followed the body is removed.

Furthermore, the liquid containing groove is connected with the body through a magnetic adsorption structure, or a movable clamping groove structure, or a buckle structure; one side of the upper cover of the body is provided with at least one notch which is convenient for removing the liquid containing groove.

Furthermore, the liquid containing groove extends to exceed the area of the cleaning water tank in the length direction and/or the width direction, and the capacity of the liquid containing groove is expanded.

Furthermore, an annular bulge is arranged at the opening position of the cleaning water tank on the upper cover of the body; the opening of the cleaning water tank is clamped with a water tank sealing cover.

Further, the body upper cover is kept away from one side of body base is equipped with the mounting groove, the electrophoresis is separated the storehouse and can be installed with magnetism adsorption mode or buckle mode in the mounting groove, in the mounting groove with the electrophoresis dissociates the probe and corresponds the position and is equipped with the switching spring needle, the switching spring needle with the circuit board electricity is connected the electrophoresis is separated the storehouse and is installed when in the mounting groove, the switching spring needle can rather than corresponding the electrophoresis dissociates the probe connection.

Furthermore, each group of electrophoresis dissociation probes in the electrophoresis dissociation bin at least comprises a first probe and a second probe, the first probe and the second probe are arranged at intervals, and the hard corneal contact lens can be placed between the first probe and the second probe.

Further, a hard corneal contact lens and an electrolyte solution are placed in the electrophoresis dissociation bin, the hard corneal contact lens protein removal sterilization device is started, a first probe and a second probe in the electrophoresis dissociation bin form a positive probe and a negative probe under a circuit loop, protein attached to the surface of the hard corneal contact lens to be cleaned and in an oxygen permeation hole is charged in the electrolyte solution, and charged tear protein moves towards the position of the electrophoresis dissociation probe opposite to the electrical property of the charged tear protein;

cl & lt- & gt in the electrolyte solution moves towards the positive probe, electrons lost are oxidized into chlorine, the chlorine is dissolved in the electrolyte solution to generate hypochlorous acid, the hypochlorous acid and protein are subjected to redox reaction in the electrophoresis dissociation bin, and the tear protein is degraded;

hypochlorous acid performs self-redox reaction in the electrolyte solution to decompose hydrogen ions, chloride ions and oxygen, and absorbs electrons of functional proteins of microorganisms in the electrophoretic dissociation bin during decomposition so as to inactivate the functional proteins of the microorganisms, so that the microorganisms are killed.

Further, the electrolyte solution is a solution containing NaCl,

the positively charged hydrogen ions in the NaCl-containing solution move to the negative probe and undergo a reduction reaction near the negative probe to generate hydrogen, sodium ions and hydroxyl ions in the electrolyte solution generate sodium hydroxide, the grease on the surface of the hard corneal contact lens undergoes a saponification reaction with the sodium hydroxide, and the grease on the surface of the hard corneal contact lens is removed;

the hypochlorous acid and the protein are subjected to redox reaction in the electrophoretic separation chamber, and the protein is degraded, and the method specifically comprises the following steps:

reacting hypochlorous acid with a peptide chain forming a protein skeleton to obtain chloral formamide, hydrolyzing the peptide chain formed by the chloral formamide in a solution, breaking peptide bonds in the peptide chain, decomposing the peptide chain into micromolecule protein and amino acid, degrading the protein, and/or,

the hypochlorous acid reacts with the side chains of the peptide chains forming the protein skeleton, the side chains comprise lysine side chains, the hypochlorous acid reacts with the lysine side chains to form chloramines on the lysine side chains of the protein and the amino groups of the protein, the chloramines are decomposed to form organic molecule fragments [ 1,2 ] in a carbonyl form, the peptide bonds on the lysine side chains are broken and decomposed into small-molecule proteins and amino acids, and the proteins are degraded,

the microorganisms comprise bacteria and viruses, the hypochlorous acid absorbs electrons of functional proteins on cell walls of the bacteria in a decomposition process, and the bacteria are inactivated;

the hypochlorous acid absorbs electrons of functional proteins on the outer shell of the virus in a decomposition process, and the virus is inactivated;

the hypochlorous acid has strong oxidizing property, permeates into the microbial cells, and is subjected to oxidation reaction with mycoprotein, nucleic acid and enzyme in the microbial cells so as to kill the microbes.

According to another aspect of the present invention, the present invention also provides a method for deproteinizing and sterilizing a hard contact lens, which comprises: adding Cl-containing solution into the electrophoresis separation chamber of the deproteinization and sterilization device of the hard corneal contact lens^-The electrolyte solution and the hard contact lens to be cleaned are mixed, and a switch of the protein removing and sterilizing device of the hard contact lens is opened to remove the protein and sterilize the hard contact lens; after the rigid corneal contact lens protein removal and sterilization device works for a set time, putting the cleaned rigid corneal contact lens into a cleaning water tank; flushing the hard corneal contact lens in the cleaning water tank by using a flushing tool until the electrolyte solution on the hard corneal contact lens is completely flushed; and the washing liquid after washing flows into the liquid containing groove through the hollow part of the washing water groove.

Furthermore, when a switch of the protein removing and sterilizing device of the hard corneal contact lens is turned on, a first probe and a second probe in the electrophoresis dissociation bin form a positive probe and a negative probe under a circuit loop, tear protein attached to the surface of the hard corneal contact lens to be cleaned and the oxygen permeation holes is charged in an electrolyte solution, and the charged tear protein moves towards the position of the electrophoresis dissociation probe opposite to the electrical property of the charged tear protein; cl in the electrolyte solution^-Moving towards the positive probe, oxidizing the lost electrons into chlorine, dissolving the chlorine in the electrolyte solution to generate hypochlorous acid, carrying out redox reaction on the hypochlorous acid and the lachrymarin in the electrophoretic separation bin, and degrading the lachrymarin; hypochlorous acid performs self-redox reaction in the electrolyte solution to decompose hydrogen ions, chloride ions and oxygen, and absorbs electrons of functional proteins of microorganisms in the electrophoretic dissociation bin during decomposition so as to inactivate the functional proteins of the microorganisms, so that the microorganisms are killed.

Furthermore, the electrolyte solution is a solution containing NaCl, hydrogen ions with positive charges in the electrolyte solution move to the negative probe, and a reduction reaction is performed near the negative probe to generate hydrogen gas, sodium ions and hydroxyl ions in the electrolyte solution generate sodium hydroxide, oil on the surface of the hard corneal contact lens and the sodium hydroxide perform a saponification reaction, and the oil on the surface of the hard corneal contact lens is removed.

Compared with the prior art, the method has one or more of the following advantages:

(1) the application discloses a protein removing and sterilizing device for hard corneal contact lenses, which adopts the technology of combining the protein electrophoresis principle and the electrolysis technology and is matched with Cl^-The electrolyte solution can effectively separate dirt such as tear protein on the hard corneal contact lens from the hard corneal contact lens, and can generate hypochlorous acid in an electrophoresis separation bin, so that the tough sedimentary protein on the hard corneal contact lens can be decomposed into micromolecular protein and amino acid to facilitate electrophoresis adsorption, and bacteria and pathogenic microorganisms can be killed to achieve double effects of protein removal and sterilization;

(2) the electrolyte solution used by the device for deproteinizing and sterilizing the hard corneal contact lens can be any Cl-containing solution without heavy metals^-The solution, such as physiological saline, common care solution and the like, has strong applicability;

(3) the protein removing and sterilizing device for the hard corneal contact lens is provided with the electricity storage device, is convenient to carry and can be normally used in environments without power supplies such as the road and the like;

(4) the protein removing and sterilizing device for the hard corneal contact lens is provided with the cleaning water tank and the liquid containing tank, and can clean the corneal contact lens cleaned from the electrophoresis dissociation bin at any time and any place by matching with a cleaning tool such as a spray can and the like, so that a user does not need to separately hold the whole device or the corneal contact lens to a place such as a water tank for cleaning again, the cleaning convenience is greatly improved, and the defect that the corneal contact lens cannot be used due to the fact that no cleaning condition exists at the side is overcome;

(5) according to the device for deproteinizing and sterilizing the hard corneal contact lens, the special cleaning solution is sprayed to the corneal contact lens at each angle through flushing tools such as a spray can, so that the lens can be prevented from being damaged or broken due to rubbing by hands;

(6) according to the protein removing and sterilizing device for the hard corneal contact lens, the liquid containing groove is detachably designed, and the notch which is convenient for removing the liquid containing groove is formed between the liquid containing groove and the upper cover of the body, so that the washed waste liquid can be conveniently and conveniently poured after the corneal contact lens is washed;

(7) the protein removing and sterilizing device for the hard corneal contact lens has the advantages that the protein removing effect can be nearly 100%, and the risk that human eyes are infected by bacteria can be effectively reduced.

Drawings

Fig. 1 is a schematic structural diagram of a rigid contact lens deproteinizing and sterilizing apparatus provided in the embodiment of the present application during separation in an electrophoresis dissociation chamber;

FIG. 2 is an exploded view of a rigid contact lens deproteinizing and sterilizing apparatus according to an embodiment of the present disclosure;

fig. 3 is a schematic structural diagram of a rigid corneal contact lens deproteinizing and sterilizing apparatus provided in the embodiment of the present application in a top view direction when the electrophoresis dissociation chamber is separated;

FIG. 4 is a schematic top view of a rigid contact lens deproteinizing and sterilizing apparatus according to an embodiment of the present disclosure;

FIG. 5 is a cross-sectional view of a rigid contact lens deproteinizing and sterilizing apparatus provided in the embodiments of the present application at FIG. 4A;

FIG. 6 is a cross-sectional view of a hard contact lens deproteinizing and sterilizing apparatus provided in the embodiments of the present application at FIG. 4B;

FIG. 7 is a schematic structural diagram of a rigid contact lens deproteinizing and sterilizing apparatus according to an embodiment of the present application, which is shown in a top view when the cover is removed;

FIG. 8 is a cross-sectional view of a rigid contact lens deproteinizing and sterilizing apparatus provided in accordance with an embodiment of the present application at FIG. 7D;

FIG. 9 is a cross-sectional view of a rigid contact lens deproteinizing and sterilizing apparatus provided in accordance with an embodiment of the present application at FIG. 7C;

FIG. 10 is a schematic structural diagram of a rigid contact lens deproteinizing apparatus provided in the embodiment of the present application when the liquid holding tank is separated;

FIG. 11 is a schematic view of the bottom view of the installation position of the rigid contact lens deproteinizing apparatus in the liquid containing tank when the liquid containing tank is removed;

FIG. 12 is a schematic structural diagram of a liquid containing tank according to an embodiment of the present disclosure;

fig. 13 is a schematic structural view of the liquid containing tank provided in the embodiment of the present application in a front view direction;

FIG. 14 is a schematic side view of the liquid containing tank according to the embodiment of the present disclosure;

FIG. 15 is a schematic structural view of the liquid containing tank in a bottom view according to the embodiment of the present disclosure;

FIG. 16 is an electron micrograph showing degradation of lysozyme by the 3N-based care solution in verification example 2 by 12% SDS-PAGE;

FIG. 17 is an electron micrograph showing degradation of lysozyme by a commercial hard contact lens deproteinizing solution of validation example 2 using 15% SDS-PAGE.

Wherein, 1-body, 11-body upper cover, 111-cleaning water tank, 1111-basket structure, 112-notch, 113-annular protrusion, 114-mounting groove, 115-switching spring needle, 116-first inner side wall, 117-second inner side wall, 118-strip clamping protrusion, 119-bayonet, 12-body base, 13-circuit PCBA mainboard, 14-circuit PCBA subplate, 2-electrophoresis dissociation chamber, 21-electrophoresis dissociation probe, 22-electrophoresis dissociation base, 23-electrophoresis dissociation upper cover, 231-left electrophoresis dissociation chamber, 232-right electrophoresis dissociation chamber, 3-liquid containing tank, 31-strip clamping groove, 32-clamping hook, 4-cover cap, 5-sealing cover cap, 6-anti-skid pad, 7-tool groove, 71-wearing tools, 8-shading foam.

Detailed Description

To further explain the technical means and effects of the present invention adopted to achieve the predetermined objects, the following detailed description of the embodiments, structures, features and effects according to the present invention will be given with reference to the accompanying drawings and preferred embodiments.

Examples

The present embodiment provides a rigid corneal contact lens deproteinizing and sterilizing device, as shown in fig. 1 to 10, comprising: a body 1, an electrophoresis separating bin 2 and a liquid containing tank 3. What is neededThe body 1 is internally provided with an accommodating cavity, an electricity storage device, a circuit board and a switch assembly are accommodated in the accommodating cavity, the electricity storage device and the switch assembly are respectively electrically connected with the circuit board, and one side of the body 1 is provided with a cleaning water tank 111. The arrangement of the electric storage device enables the protein removing and sterilizing device of the hard corneal contact lens to be more convenient to carry and can be normally used in environments lacking power supplies such as on the road. Electrophoresis dissociation storehouse 2 is located one side of body 1, at least, be provided with a set of electrophoresis dissociation probe 21 in the electrophoresis dissociation storehouse 2, electrophoresis dissociation storehouse 2 intussuseption is filled with and contains Cl^-The electrophoretic dissociation probe 21 and the Cl-containing compound^-The electrophoretic dissociation probe 21 is electrically connected to the circuit board. The liquid tank 3 is disposed at the other side of the body 1, and the position of the liquid tank 3 corresponds to the position of the cleaning water tank 111. In the present embodiment, two circuit boards, one of which is a circuit PCBA main board 13 and the other of which is a circuit PCBA sub-board 14, are preferably used, and the two circuit boards are electrically connected.

The cleaning water tank 111 is designed to be hollow, and an opening of the cleaning bin faces the cleaning water tank 111. As shown in fig. 1 to 3, 7, 10, or 11, the cleaning water tank 111 shown in the figures is in a racetrack shape, and two hollowed-out circular basket structures 1111 which are recessed downward are arranged in the cleaning water tank 111. One for placing a left eye contact lens and the other for placing a right eye contact lens for differentiation. Of course, in specific implementation, the cleaning water tank 111 may have other shapes, and the shape, size and number of the hollow parts may be designed as required, as long as the liquid in the cleaning water tank 111 can flow into the liquid containing tank 3 below the cleaning water tank 111. The cleaning water tank 111 can facilitate the washing of the hard corneal contact lens after the deproteinization and sterilization in the electrophoretic separation chamber 2, and the washed cleaning solution can flow into the liquid containing tank 3 through the hole in the cleaning water tank 111. This structural design, the cooperation flushing tool such as watering can alright wash the cornea contact lens that has washed in the follow electrophoresis dissociation storehouse 2 anytime and anywhere, need not the user to hold whole device or cornea contact lens again alone and wash again to places such as pond, greatly increased abluent convenience, also solved simultaneously the condition of not washing on one's side and lead to the drawback that can't use the cornea contact lens.

In a further embodiment, the body 1 comprises a body upper cover 11 and a body base 12, and the liquid containing tank 3 is positioned on one side of the body base 12. As shown in fig. 2 or fig. 11, the body base 12 shown in the figures is a truncated rectangular parallelepiped shape, the shape of the truncated corner is the same as that of the liquid containing tank 3, and the liquid containing tank 3 is arranged at the position of the truncated corner of the body base 12, and just forms a complete rectangular parallelepiped structure with the body base 12. The shape of the upper cover 11 of the body is consistent with the shape of the combined body base 12 and the liquid containing tank 3, and the upper cover of the body 1 can be just completely clamped on the body base 12 and the liquid containing tank 3. As can be seen from the figure, the upper body cover 11 comprises two parts, wherein one part of the upper part is protruded upwards, a larger cavity is formed between the upper part and the base 12 of the body, and a plurality of circuit PCBA mainboards 13, power storage devices or other components used by the device, such as display lamps, switch components, light shielding foam 8 and the like, can be arranged in the upper body cover. The other part is a lower part, the electrophoresis separation chamber 2 and the cleaning water tank 111 are both arranged at the lower part of the upper cover 11 of the body, and the circuit PCBA auxiliary board 14 is arranged below the electrophoresis separation chamber 2. In order to make the whole device more beautiful, in practice, the electrophoretic separation chamber 2 may be symmetrically arranged with respect to the cleaning water tank 111.

In a further embodiment, the liquid container 3 and the body base 12 are of a separate structure, i.e. there is a separate structure between the liquid container 3 and the body base 12. The body upper cover 11 is clamped on the body base 12, a sealing ring can be arranged between the port of the body base 12 and the body upper cover 11 for water proofing. Of course, the sealing of the chamber between the body base 12 and the body cover 11 can also be achieved by other means, such as sealing glue between the port of the body base 12 and the body cover 11. Therefore, the liquid containing groove 3 is completely separated from the cavity formed between the body base 12 and the body upper cover 11, namely, the liquid containing groove 3 and the elements and circuits in the whole device are independently separated, and the waterproof effect can be effectively ensured.

In a further embodiment, the liquid containing groove 3 is connected with the body 1 by a magnetic adsorption structure, or a movable clamping groove structure, or a buckling structure. Preferably, the liquid containing tank 3 is installed below the body upper cover 11 in a manner of combining a movable clamping groove structure and a buckling structure. As shown in fig. 11, the position of the body top cover 11 corresponding to the sidewall of the unfilled corner of the body base 12 is provided with an inner sidewall structure corresponding to the sidewall, that is, a first inner sidewall 116 along the length direction of the device and a second inner sidewall 117 along the width direction of the device. When the body upper cover 11 is clamped on the body base 12, the first inner side wall 116 and the second inner side wall 117 of the body upper cover 11 can just fit with the side walls at the corners of the body base 12, so that a complete cavity is formed between the body upper cover 11 and the body base 12. The movable clamping groove structure adopts a structure that the strip-shaped clamping protrusion 118 is matched with the strip-shaped clamping groove 31, and the clamping structure adopts a connecting structure that the clamping hook 32 is matched with the clamping opening 119. The liquid containing tank 3 shown in the figure is drawn along the width direction of the device, so that two bayonets 119 are arranged on the first inner side wall 116, a strip-shaped protrusion is arranged on the second inner side wall 117 along the width direction of the device, and in order to ensure the installation stability of the liquid containing tank 3, another strip-shaped protrusion is arranged at the corresponding position of the end part of the upper cover 11 of the body. And strip-shaped grooves corresponding to the strip-shaped bulges are respectively arranged at the positions, close to the opening, of the two wide side surfaces of the liquid containing groove 3. One end of the strip-shaped groove penetrates through the mounting surface of the liquid containing groove 3, as shown in fig. 12 to 15. Through the cooperation between the strip-shaped protrusions and the strip-shaped grooves, the liquid containing groove 3 can be pulled and pulled along the width direction of the device below the body upper cover 11. Meanwhile, a hook 32 is arranged at a position close to the opening on the mounting surface of the liquid containing tank 3, one bayonet 119 corresponds to one hook 32, and when the liquid containing tank 3 is mounted below the body upper cover 11, the hook 32 on the liquid containing tank 3 is clamped with the corresponding bayonet 119, so that the liquid containing tank 3 can be firmly fixed below the body upper cover 11, namely below the cleaning water tank 111. It should be understood that the above are preferred embodiments, and in specific implementation, the drawing direction of the liquid containing tank 3 is not limited to the width direction of the device, and may also be the length direction of the device, and the same technical effect can also be achieved by synchronously making adaptive adjustment on the positions of the slot structure and the buckle structure.

The buckle structure in the above scheme can also be replaced by a magnetic adsorption structure, for example, a magnetic structure made of magnetically attractable materials such as magnets or iron blocks is injected into the mounting surface of the liquid containing tank 3, and simultaneously, the magnetic structure made of magnetically attractable materials such as magnets or iron blocks is injected into the inner side wall or is installed at a position of the body base 12 close to the inner side wall, and of course, the magnetic structure between the liquid containing tank 3 and the inner side wall is corresponding, i.e., the magnetic structure between the two is capable of being attracted. When the liquid containing tank 3 is close to the installation position to a certain degree, the installation surface of the liquid containing tank 3 is firmly attracted on the inner side wall under the action of magnetic force. It should be noted that the liquid containing tank 3 is mounted on the upper cover 11 of the body only as a preferred solution, and in specific implementation, the liquid containing tank 3 may also be mounted on the base 12 of the body, that is, the fastening structure, the movable slot structure or the magnetic adsorption structure is disposed on the sidewall of the unfilled corner of the base 12 of the body, and the technical principle is substantially the same as the above technical solution, and is not described herein again.

Above magnetic adsorption structure all realizes based on movable draw-in groove structure, when concrete implementation certainly, liquid containing groove 3 also can adopt the mode of complete magnetic adsorption to install the below of body upper cover 11. For example, a magnetic structure is also molded on the opening end face of the liquid container 3, and another magnetic attraction structure capable of attracting the magnetic structure at the opening end of the liquid container 3 is installed or molded on the corresponding position of the body upper cover 11. With the structure design, the drawing direction of the liquid containing groove 3 is not limited in the transverse direction, and can also be in the longitudinal direction, so that the taking and placing of the liquid containing groove 3 are more convenient. Certainly, the liquid containing tank 3 may also be mounted below the body upper cover 11 in a complete fastening manner, that is, a plurality of upward hooks 32 are arranged around the opening of the liquid containing tank 3, and a plurality of bayonets 119 corresponding to the hooks 32 are arranged at corresponding positions of the body upper cover 11, so that the liquid containing tank 3 can be mounted below the body upper cover 11 in a detachable manner.

In a further embodiment, the body cover 11 is provided with at least one notch 112 on one side for facilitating the removal of the liquid tank 3. The notch 112 is formed at the connection position of the upper cover 11 and the liquid containing tank 3, the notch 112 can accommodate the insertion of fingers, and a user can conveniently remove or take down the liquid containing tank 3 from the body 1 through the notch 112.

In a further embodiment, the liquid containing groove 3 extends beyond the area of the cleaning water tank 111 in the length direction and/or width direction thereof, expanding the capacity of the liquid containing groove 3. Through reasonable structure and size design, the volume of the liquid containing groove 3 is large enough to ensure that the liquid level of the cleaning liquid flowing into the liquid containing groove 3 can not reach the bottom end of the cleaning water groove 111 and can not cause secondary pollution to the hard corneal contact lens when the hard corneal contact lens in the cleaning water groove 111 is cleaned.

In a further embodiment, the upper body cover 11 is provided with an annular protrusion 113 at the opening of the cleaning water tank 111, so as to ensure that the cleaning liquid does not overflow from the cleaning water tank 111 during the cleaning process.

In a further embodiment, a water tank sealing cover can be fastened at the opening of the cleaning water tank 111, the cleaning water tank 111 can be covered normally, dust is prevented from falling into the cleaning water tank, and the water tank sealing cover can be removed or opened when a washing tool such as a watering can is used for washing. Preferably, the sink sealing cover is rotatably installed on the body upper cover 11, and a side surface of the sink sealing cover may also function as a liquid splash barrier when the sink sealing cover is opened during washing.

In a further embodiment, the body upper cover 11 is kept away from one side of the body base 12 is provided with a mounting groove 114, the electrophoresis dissociation chamber 2 can be installed in the mounting groove 114 in a magnetic adsorption mode or a buckling mode, the mounting groove 114 is internally provided with an adapter spring needle 115 corresponding to the electrophoresis dissociation probe 21, the adapter spring needle 115 is electrically connected with the circuit board, the electrophoresis dissociation chamber 2 is installed in the mounting groove 114, the adapter spring needle 115 can be connected with the electrophoresis dissociation probe 21 corresponding thereto. Electrophoresis is separated storehouse 2 and is included electrophoresis base 22 and the electrophoresis upper cover 23 that dissociates, electrophoresis dissociates upper cover 23 block and is in one side of electrophoresis base 22 that dissociates, electrophoresis dissociates upper cover 23 and keeps away from one side of electrophoresis base 22 that dissociates is equipped with left electrophoresis dissociation groove 231 and right electrophoresis dissociation groove 232, electrophoresis dissociation probe 21 locates in the electrophoresis base 22 that dissociates, just the one end of electrophoresis dissociation probe 21 is passed electrophoresis base 22 that dissociates keeps away from one side and the outside intercommunication of electrophoresis dissociation upper cover 23, electrophoresis dissociation probe 21's the other end extends to left electrophoresis dissociation groove 231 or right electrophoresis dissociation groove 232, and can with electrolyte solution contact in left electrophoresis dissociation groove 231 or the right electrophoresis dissociation groove 232. When the electrophoretic separation chamber 2 is installed in the installation groove 114 in a magnetic adsorption manner, magnetic attraction means may be respectively disposed in the electrophoretic separation base 22 and the body upper cover 11. Preferably, the magnetic attraction device is a magnet, and one or more magnetic blocks are arranged at the bottom end of the electrophoretic dissociation base 22 and below the mounting groove 114 of the upper body cover 11, or may be in the shape of a magnetic ring or the like. Of course, it is also possible to arrange one magnet and the other iron piece in the electrophoretic dissociation base 22 and the upper cover 11 of the body, or other magnetically attractable devices, etc., all of which are within the scope of the present application. When electrophoresis is separated storehouse 2 and is installed with the buckle mode in mounting groove 114, this buckle mode can be a point and the cooperation structure between the recess, for example set up a plurality of bump in electrophoresis is separated the side in storehouse 2, and set up the recess in mounting groove 114's inside wall corresponding position, when electrophoresis is separated storehouse 2 and is installed in mounting groove 114, the bump just can the block in corresponding the recess. Of course, a plurality of grooves may be formed on the side surface of the electrophoretic separation chamber 2, and bumps may be formed at corresponding positions on the inner side wall of the mounting groove 114, so that the same fastening effect can be achieved. This embodiment still provides another kind of buckle mode, for example electrophoresis is separated the both sides of storehouse 2 and is set up the pothook, set up on body upper cover 11 with the bayonet socket that the pothook corresponds when electrophoresis is separated storehouse 2 and is installed in mounting groove 114, the pothook is rather than the bayonet socket cooperation connection that corresponds.

Electrophoresis is separated storehouse 2 with can also set up limit structure between the mounting groove 114, limit structure includes spacing arch and spacing recess, can be electrophoresis is separated the bottom surface of storehouse 2 and is set up one or more spacing archs the bottom of mounting groove 114 correspond the position set up with the spacing recess that spacing protruding shape corresponds works as electrophoresis is separated storehouse 2 and is installed when in the mounting groove 114, spacing protruding block is in the spacing recess, the effectual effect that plays spacing or direction electrophoresis and separate storehouse 2. Of course, one or more limiting grooves may be disposed on the bottom surface of the electrophoretic separation chamber 2, and a limiting protrusion having a shape corresponding to that of the limiting groove is disposed at a corresponding position on the bottom of the mounting groove 114, which may also have the same limiting and guiding functions.

In a further embodiment, each set of electrophoretic dissociation probes 21 in the electrophoretic dissociation chamber 2 comprises at least a first probe and a second probe, the first probe and the second probe are arranged at intervals, and a rigid corneal contact lens can be placed between the first probe and the second probe. Preferably, a set of electrophoretic dissociation probes 21 is disposed in each of the left electrophoretic dissociation tank 231 and the right electrophoretic dissociation tank 232, and each set includes a first probe and a second probe. The first probe and the second probe are respectively connected with positive and negative electrode access ports on the circuit PCBA sub-board 14, so that one of the first probe and the second probe is used as a positive electrode probe, the other probe is used as a negative electrode probe, and the positive and negative electrodes of the first probe and the second probe can be adjusted according to needs.

In a further embodiment, the sealing caps 5 are fastened to the left electrophoresis dissociation tank 231 and the right electrophoresis dissociation tank 232, and when the hard contact lens is cleaned, the sealing caps 5 can be used to seal the left electrophoresis dissociation tank 231 and the right electrophoresis dissociation tank 232, so as to prevent the electrolyte solution from leaking out of the electrophoresis dissociation tanks.

In a further embodiment, the rigid contact lens deproteinizing sterilization device further comprises an anti-slip pad 6, wherein the anti-slip pad 6 is arranged on one side of the body base 12 far away from the body upper cover 11.

In a further embodiment, the body cover 11 is further provided with a tool recess 7. For placing some wearing tools 71, such as tweezers, sucker rods, etc. As shown in fig. 1 to 3, the tool recess 7 is provided at a side of the lower portion of the body cover 11 away from the body base 12, and the tool recess 7 is located between the mounting groove 114 or the storage groove and the upper portion of the body cover 11. Through tools such as tweezers and sucker rods, the corneal contact lens in washing can be moved or turned over in an auxiliary manner when the corneal contact lens is washed in the washing water tank 111, so that the washing is convenient. Simultaneously, the hard contact lens except that albumen sterilizing equipment of this application is in the lower part of body upper cover 11 still blocks there is a shroud 4 behind the shroud 4 block, the top of shroud 4 with the upper end parallel and level of the higher part of body upper cover 11 can guarantee shroud 4 and protect its in-device each part or structure not to fall grey while, can also guarantee the holistic aesthetic property of device.

The embodiment also provides a method for removing protein and sterilizing the hard corneal contact lens, which specifically comprises the following steps:

adding an electrolyte solution containing Cl < - > and a hard contact lens to be cleaned into an electrophoresis dissociation chamber 2 of a hard contact lens deproteinization sterilization device, opening a switch of the hard contact lens deproteinization sterilization device, forming a positive probe and a negative probe by a first probe and a second probe in the electrophoresis dissociation chamber 2 under a circuit loop, charging tear protein attached to the surface of the hard contact lens to be cleaned and an oxygen permeable hole in the electrolyte solution, and moving the charged tear protein towards the position of an electrophoresis dissociation probe 21 with the electric property opposite to that of the charged tear protein; cl < - > in the electrolyte solution moves towards the positive probe, electrons lost are oxidized into chlorine gas, the chlorine gas is dissolved in the electrolyte solution to generate hypochlorous acid, the hypochlorous acid and the lachrymarin undergo redox reaction in the electrophoretic dissociation chamber 2, and the lachrymarin is degraded. After the rigid corneal contact lens protein-removing sterilization device works for a set time, putting the cleaned rigid corneal contact lens into a cleaning water tank 111; flushing the hard corneal contact lens in the cleaning water tank 111 by using a flushing tool until the electrolyte solution on the hard corneal contact lens is completely flushed; when in washing, special washing liquid can be sprayed to the corneal contact lens from various angles by using washing tools such as a watering can and the like, so that the lens damage caused by rubbing with hands can be avoided; the washing liquid after washing flows into the liquid tank 3 through the hollow of the washing water tank 111.

The working principle of the invention is based on protein electrophoresis technology, i.e. charged particles move towards the electrode with the electric opposite to the charged particles under the action of an electric field, which is called electrophoresis. The protein is a molecule with complete biological activity formed by one or more polypeptide chains according to a respective special combination mode, and the protein is an ampholyte, when the protein is at an isoelectric point, the electrostatic charge of the protein molecule is zero, and the protein molecule is generally positively charged in a relatively acidic solution and negatively charged in a relatively basic solution. When electrolyte solution is added into the electrophoresis separation chamber 2, when the first probe and the second probe are electrified by the circuit board, a passage is formed between the first probe and the second probe through the electrolyte solution, and a certain potential difference is formed between the first probe and the second probe, filth such as tear protein and the like adhered on the hard corneal contact lens has a certain charge in the electrolyte solution, is separated from the hard corneal contact lens under the action of the potential difference, moves to the first probe or the second probe in the electrolyte solution, and is finally adsorbed on the surface of the first probe or the second probe, so that the hard corneal contact lens is cleaned. And with a compound containing Cl^-When the device is powered on, Cl in the electrolyte solution^-Move towards the positive probe, lose electrons and are oxidized into chlorine, the chlorine is dissolved in the electrolyte solution to generate hypochlorous acid, the hypochlorous acid and the lachrymarin can generate oxidation-reduction reaction in the electrophoretic separation chamber 2, and the lachrymarin is degraded. Hypochlorous acid is a strong oxidizing agent that undergoes a self-redox reaction in the electrolyte solution, decomposing hydrogen ions, chloride ions and oxygen. Micro-meterOrganisms include bacteria and viruses, the hypochlorous acid absorbs electrons of functional proteins on cell walls of the bacteria during decomposition, and the bacteria are inactivated; the hypochlorous acid absorbs electrons of functional proteins on the outer shell of the virus during the decomposition process, and the virus is inactivated. The hypochlorous acid has strong oxidizing property, and can permeate into the microbial cells to perform oxidation reaction with mycoprotein, nucleic acid and enzyme in the microbial cells so as to kill the microbes.

The electrophoresis dissociation probe 21 used in this embodiment is a nano-scale plating material, the material of the electrophoresis dissociation probe 21 is corrosion resistant, therefore, the oxidation-reduction reaction generated by the electrolysis in the electrophoresis dissociation chamber 2 can not cause the corrosion of the electrophoresis dissociation probe 21, alternatively, the electrolyte solution does not react with the electrophoretic dissociation probe 21, and the electrolyte solution reacts in the vicinity of the two electrophoretic dissociation probes 21 having different electric properties (from the viewpoint of concentration, according to the theory of directional movement of ions, the concentration of ions near the electrophoretic dissociation probe 21 is higher than that at other positions in the electrophoretic dissociation chamber, but the space in the electrophoretic dissociation chamber is originally small, and the concentration of the electrolyte is constant, and the reaction can also be said to occur in the electrophoretic dissociation chamber), so that ions that break the molecular structure of the tear protein are formed, and the tear protein is electrolytically dissociated by the ions.

In one embodiment, a 0.9% NaCl solution is preferably used as the electrolyte solution for cleaning a hard contact lens. The NaCl-containing solution is electrolyzed under the action of current, sodium ions and hydrogen ions in the solution move to the negative probe, and chloride ions move to the positive probe. In the electrolysis process, hydrogen ions in the Nacl solution obtain electrons at the negative electrode probe to perform a reduction reaction to form hydrogen, chlorine ions lose electrons at the positive electrode probe to be reduced into chlorine, the Nacl solution with a certain concentration generates chlorine, a part of the generated chlorine is discharged by bubbles, and a part of the generated chlorine is dissolved in water and performs the following chemical reaction: cl₂+H₂O＝HCl+HClO

The hypochlorous acid HClO generated by the reaction has the function of degrading protein. Hypochlorous acid can decompose the sedimentary protein on the corneal contact lens into small molecular protein and amino acid, and the small molecular protein is easier to adsorb than the sedimentary protein, so that the removal of the tear protein on the corneal contact lens is facilitated. This degradation reaction is mainly of two forms: one is directly degrading a skeleton peptide chain of protein, reacting hypochlorous acid with the peptide chain forming a protein skeleton to obtain chloral formamide, hydrolyzing the peptide chain formed by the chloral formamide in a solution, breaking peptide bonds in the peptide chain, decomposing the peptide chain into micromolecular protein and amino acid, and degrading the protein; the other is that hypochlorous acid reacts with the side chain of the peptide chain forming the protein skeleton, wherein the side chain comprises a lysine side chain, and the hypochlorous acid reacts with the lysine side chain to form chloramine on the lysine side chain of the protein and the amino group of the protein, the chloramine is decomposed to form organic molecule fragments [ 1,2 ] in a carbonyl form, the peptide bond on the lysine side chain is broken, and the organic molecule fragments are decomposed into small-molecule proteins and amino acids, and the proteins are degraded.

Meanwhile, hypochlorous acid also has the functions of sterilization and disinfection: hypochlorous acid slowly generates self redox reaction to be decomposed, each hypochlorous acid molecule can absorb electrons during decomposition (hypochlorous acid is used as a strong oxidizer and can absorb electrons of surface protein of microbial cell walls, so that the surface functional protein of the microbes is oxidized, the microbes are inactivated due to incapability of taking nutrition, incapability of normal metabolism and stopping division, and finally achieve the effects of sterilization and disinfection), the aim of sterilization is fulfilled, hydrogen ions are dissociated, and chloride ions and oxygen are obtained, and the chemical formula is as follows:

2HClO→2H⁺+2Cl^-+O₂↑。

in the process of sterilization and virus killing, hypochlorous acid can act on cell walls and virus shells, has small molecules and no electric charge, can permeate into the bodies of bacteria (viruses) and can generate oxidation reaction with organic macromolecules such as bacteria (viruses) body protein, nucleic acid, enzyme and the like, thereby killing pathogenic microorganisms.

Simultaneously, the hydrogen ion of the positive charge in the electrolyte solution moves to the negative probe, and the reduction reaction is generated near the negative probe to generate hydrogen, sodium ions and hydroxyl ions in the electrolyte solution generate sodium hydroxide, the grease on the surface of the hard corneal contact lens and the sodium hydroxide are subjected to saponification reaction, and the grease on the surface of the hard corneal contact lens is removed. This application rigid contact lens removes albumen sterilization apparatus has also promoted the change of solution pH value when removing albumen sterilization effect, and when rigid contact lens was last to be attached with lipid, the electrophoresis was dissociated and is promoted going on of saponification reaction, consequently, when rigid contact lens had lipid, the application rigid contact lens removes albumen sterilization apparatus and also can be in the same direction as taking and remove lipid, can be said one-tenth multiple-purpose, washs high-efficiently, but quick-acting reduction.

The above theory proves that the electrolyte solution used in the present application for implementing the sterilization of the protein removal by electrophoretic dissociation can be any solution containing chlorine ions and not containing heavy metals, and even normal saline and ordinary care solution (basically, the care solution circulating in the market all contain chlorine ions) can be used as the electrolyte solution described in the present application, such as NaCl solution, KCl solution, MgCl solution and the like which are more conventional₂Solution, and containing Cl^-The lens care solution of (1), or a combination of a plurality of these solutions (provided that no reaction occurs and electrolysis is disrupted so that hypochlorous acid cannot be generated in the lens cleaning bath), or in general, an ionic solution containing chlorate and useful as a lens care solution, can be used as the electrolyte solution described herein. Thus, any available chloride ion-containing solution used in the present application may serve the dual purpose of protein removal and sterilization.

Compared with the method of rubbing and washing and soaking the lens by the nursing liquid in the prior art, the electrophoresis dissociation protein removal sterilization method prolongs the service life of the hard corneal contact lens, can effectively remove protein and sterilize, and can reduce the risk of bacterial infection of human eyes by the cleaning method with double protection and high cleanliness.

The hard contact lens protein removing and sterilizing device has the advantages that the tear protein attached to the hard contact lens is less or almost free for the new or short-term hard contact lens, so that the hard contact lens protein removing and sterilizing device can be used for sterilizing and sterilizing the new contact lens; for the contact lens with long service time, more lacrimal proteins are inevitably deposited on the contact lens (because human eyes are a complicated environment, more lacrimal proteins are inevitably adsorbed on the contact lens after the contact lens is used for a long time), and the contact lens cleaning device has double effects of protein removal and sterilization when the contact lens is cleaned.

Verification example 1:

verify the clearance effect of hard contact lens deproteinization sterilization apparatus of this application to protein:

the lysozyme is cleaned by using the electrophoresis dissociation protein-removing function of the hard corneal contact lens protein-removing sterilization device (the following experimental contents and data are both tested and provided by a Suzhou biological nanometer garden hundred-Tuo biological medicine public service platform):

prepare the protein solution of certain concentration, add 0.9% NaCl solution and carry out the electrophoresis experiment in the electrophoresis dissociation storehouse 2 of this application rigid corneal contact lens deproteinization sterilization apparatus, the in-process can produce hypochlorous acid, and it can degrade the protein.

The main equipment is as follows:

1. a rigid corneal contact lens protein-removing and sterilizing device;

2. micro ultraviolet spectrophotometer: nanodrop One/Onec.

Main reagent solution:

1. protein: lysozyme, Solebao (cat # L8120), molecular weight 14 KDa;

2. preparing 0.9% NaCl, testing concentration, taking 2ml for electrophoresis for 30 minutes, and detecting the concentration after electrophoresis.

Hypochlorite generated in electrophoresis affects the ultraviolet absorption of A280, and the effect can be completely eliminated by adding 10% of sodium sulfite. In order to detect proteins in the same background, a 0.9% NaCl solution without protein was used as a control for electrophoresis of proteins, and the control was also electrophoresed for 30 minutes, and 10% sodium sulfite was added to the control to perform background subtraction.

The experimental results are as follows: the protein concentration was measured 3 times and averaged to give the final concentration.

Before electrophoresis, the following three times are respectively: 657.4 μ g/mL; 651.2 μ g/mL; 649.8 μ g/mL; the average concentration is 652.8 mug/mL; after 30 minutes of electrophoresis, 1mL of the solution was taken, and 1mL of 20% sodium sulfite was added to obtain an average concentration of 99.9. mu.g/mL; 96.1 ug/mL; 98.9 mug/mL, the average is 98.3 mug/mL; since this concentration is the concentration after the sodium sulfite double dilution, the final concentration is multiplied by 2 and is 196.6. mu.g/mL.

Protein degradation rate: original concentration-residual concentration)/original concentration x 100% ═ 652.8 μ g/mL-196.6 μ g/mL)/652.8 μ g/mL = 70%.

After 30 minutes of electrophoresis, 15 microliters of sample is taken from the electrophoresis separation chamber 2, the sample loading amount of the protein is 9 micrograms, the lysozyme is not detected by SDS-PAGE (biological electrophoresis gel running), the protein is completely decomposed, namely, the clearance rate of the protein can reach 100 percent after 30 minutes of electrophoresis.

Therefore, the experimental data show that the rigid corneal contact lens protein removal and sterilization device can completely remove proteins, and the removal rate can reach 100%.

Verification example 2:

verifying the degradation effect of different care solutions on protein:

1. degradation of protein by 3N care solution:

under the condition of normal saline and electrophoresis of 3N care solution, hypochlorous acid can be generated to degrade protein. In order to make the detection result visible, the theoretical concentration of the lysozyme in the artificial tears is increased from 1.9mg/mL to 3 mg/mL.

The main equipment is as follows: a rigid corneal contact lens protein-removing and sterilizing device;

electrophoresis apparatus: Bio-Rad Mini;

the main reagents are as follows:

the method comprises the following steps: lysozyme, Solibao (cat # L8120), molecular weight 14 KDa; 0.9% NaCl, 3 mg/mL; taking 3 microliters of sample, wherein the loading amount of protein is 9 micrograms;

the saline is prepared in a hundred-rubbing mode; preparing 0.6mg/mL of lysozyme, adding 2mL of lysozyme, performing electrophoresis for 30 minutes, and taking 15 microliters of lysozyme for loading; the loading amount of protein is 9 mug;

thirdly, preparing 0.6mg/mL lysozyme from the special cleaning solution (0.9% NaCl solution) for 3N, adding 2mL of lysozyme, performing electrophoresis for 30 minutes, and taking 15 microliters of sample. The loading amount of protein is 9 mug;

fourthly, protein molecular weight standard: thermo Fisher, cat #: 26619.

fifthly, carrying out SDS-PAGE electrophoresis buffer solution: shanghai worker, goods number: c520001-0500.

Sixthly, carrying out SDS-PAGE protein electrophoresis sample loading buffer: shanghai production: the goods number is: c508320-0001.

Liquid for staining for Bare and SDS-PAGE: shanghai worker, goods number: c900300-0100.

And, SDS-PAGE destaining: shanghai worker, goods number: c900299-0300.

Self-check, SDS-PAGE gels: shanghai worker, goods number: c661102-0001.

Lysozyme: 0.9% NaCl, 3 mg/mL; taking 3 microliters of sample, wherein the loading amount of protein is 9 micrograms;

② lysozyme: 0.9 percent NaCl with the concentration of 3mg/mL is soaked in normal saline for 30 minutes to run glue; the loading amount of protein is 9 mug;

③ preparing 0.6mg/mL lysozyme from physiological saline, adding 2mL of lysozyme for electrophoresis for 30 minutes, and taking 15 microliters of sample; the loading amount of protein is 9 mug;

fourthly, preparing 0.6mg/mL of lysozyme by 3N special cleaning solution (0.9 percent NaCl solution), adding 2 milliliters of lysozyme for electrophoresis for 30 minutes, and taking 15 microliters of lysozyme for sampling. The loading amount of protein is 9 mug;

protein molecular weight standard.

The experimental results are as follows: as shown in FIG. 16, an electron microscope image obtained by detecting the degradation of lysozyme by 3N nursing solution by 12% SDS-PAGE shows that both lysozyme prepared by 0.9% NaCl and 3N special cleaning solution (0.9% NaCl solution) degrade protein, and no protein band is seen.

2. Degradation of proteins by other commercially available care solutions:

the main reagents are as follows: a, care solution: 30 minutes of a certain brand protein removal combined solution;

b, care solution: removing protein and hydrogen peroxide within 2 hours.

The method comprises the following steps: lysozyme, Solibao (cat # L8120), molecular weight 14 KDa;

sample treatment of A care solution: preparing 0.6mg/mL of lysozyme, adding 2mL of lysozyme, soaking for 30 minutes, and taking 15 microliters of lysozyme for sampling; the loading amount of protein is 9 mug;

b, sample treatment of the care solution: preparing 0.6mg/mL of lysozyme, adding 2mL of lysozyme, soaking for 2 hours, and taking 15 microliters of lysozyme for sampling; the loading amount of protein is 9 mug;

and thirdly, protein molecular weight standard: thermo Fisher, cat #: 26619.

fourth, SDS-PAGE electrophoresis buffer: shanghai worker, goods number: c520001-0500.

Fifthly, carrying out SDS-PAGE protein electrophoresis loading buffer solution: and (3) Shanghai worker: the goods number is: c508320-0001.

Sixthly, dyeing liquid SDS-PAGE: shanghai worker, goods number: c900300-0100.

Liquid for destaining sirtuin and SDS-PAGE: shanghai worker, goods number: c900299-0300.

And, SDS-PAGE gels: shanghai worker, goods number: c661103-0001.

Nursing liquid A: preparing 0.6mg/mL of lysozyme, adding 2mL of lysozyme, soaking for 30 minutes, and taking 15 microliters of lysozyme for sampling; the loading amount of protein is 9 mug;

b, care solution: preparing 0.6mg/mL of lysozyme, adding 2mL of lysozyme, soaking for 2 hours, and taking 15 microliters of lysozyme for sampling; the loading amount of protein is 9 mug;

③ preparing 0.6mg/mL lysozyme from the care solution B, and taking 15 microliters to immediately load the lysozyme; the loading amount of protein is 9 mug;

and fourthly, protein molecular weight standard.

The experimental results are as follows: as shown in fig. 17, which is an electron micrograph obtained by detecting the degradation of lysozyme by the deproteinizing solution of commercial hard corneal contact lenses in the market by 15% SDS-PAGE, it can be seen that lysozyme in the care solution a is prepared to be 0.6mg/mL, most of the protein is degraded after soaking for 30 minutes, and only a weak band remains; after the care solution B is soaked for 2 hours, protein bands are hardly degraded compared with a control.

In conclusion, comparing the degradation of lysozyme by the 3N care solution and other commercial hard corneal contact lens deproteinizing solutions, it can be found that the care solution or the electrolyte solution for deproteinizing is very important for the degradation of the final protein, and the effect of deproteinizing by using the 3N care solution is indeed better than that of other care solutions.

Verification example 3:

verify the situation that the hard corneal contact lens deproteinization sterilization device of the application clears the protein on the corneal contact lens: in the present validation example, a protein extract solution is used to extract proteins on a corneal contact lens, and the protein extract solution is formed by mixing 50 parts of acetonitrile, 50 parts of deionized water and 0.2 part of 100% trifluoroacetic acid.

First, it is noted that the substance known at the present stage to extract proteins is a pbs solution (phosphate buffer solution, a standard salt solution), but it also has a drawback that it cannot extract proteins sufficiently from a contact lens. Therefore, there is no known method for testing the content of the protein actually adsorbed on the contact lens worn by human eyes, but in order to prove the effectiveness of the protein-removing and sterilizing device for the hard contact lens, the experimental example performs an experiment on the contact lens cultured by artificial tears, and on the premise that there is no method for specifically measuring the content of the protein actually adsorbed on the contact lens, the experiment defaults that the actual content of the protein adsorbed on the contact lens cultured by artificial tears is the theoretical content of the protein adsorbed on the lens obtained through calculation.

The verification experiment procedure for the above discussion is as follows:

1. mixing 50 parts of acetonitrile, 50 parts of deionized water and 0.2 part of 100% trifluoroacetic acid to obtain a protein extracting solution;

2. taking an FDA classified class IV soft hydrophilic contact lens, culturing in a centrifuge tube containing 1ml of artificial tears (2.2mg/ml) at constant temperature of 37 ℃ for 1 day, taking out the lens, actually measuring the protein concentration in the centrifuge tube to be 1.328mg/ml, and the theoretical value of the protein content absorbed by the lens to be 2.2-1.328-0.872 mg,

3. the lens cultured with the artificial tear in the step 2 was sufficiently dissolved in 1ml or 4ml of the protein extract, and the lens was vibrated at room temperature for 24 hours, and the protein concentration was actually measured to be 0.805mg/ml after the vibration extraction, and therefore, the protein extraction rate of the protein extracted with the protein extract was calculated to be 0.805/0.872 ═ 100% ═ 92.3%.

The protein extraction ability of the protein extract solution used in this example was obtained by the above-described experimental verification, and therefore, an experiment for extracting and detecting the protein concentration using the protein extract solution was possible.

The main principle attached to the detection method in the verification example is that tear protein adsorbed on the surface and in the inner oxygen permeable holes of the corneal contact lens can be extracted by 100% according to a mixed solution of acetonitrile-trifluoroacetic acid-water, the protein extraction solution does not react with corneal contact lens materials and does not influence the measured value of protein, so that the corneal contact lens used as a detection sample can be placed in the protein extraction solution to extract and separate protein by vibration, the protein concentration in the protein extraction solution is detected by a micro ultraviolet spectrophotometer, and the content value of the lens adsorbed protein can be obtained by simply calculating the protein concentration.

Experiments are carried out based on the detection method, and the following tests prove that the protein removing and sterilizing device for the hard corneal contact lens respectively removes proteins on an OK lens and an RGP lens:

1. the situation that the hard corneal contact lens protein removal and sterilization device removes proteins on an OK lens is verified:

the OK lens selected in the verification example is Euclid Systems Corporation Euclid rigid cornea plastic lens, which is 20163220204, LOT No. 16309PTC004 and is made of fluorinated siloxane polymer; the electrolyte solution is selected as normal saline, namely 0.9% NaCl solution, and the specific method is as follows:

(1) after the human eyes wear the OK glasses for 8 hours, taking down the left and right glasses, respectively putting the left and right glasses into a 1.5ml centrifugal tube, adding 1ml acetonitrile-trifluoroacetic acid solution into the centrifugal tube, vibrating for 30 minutes, testing the lysozyme amount in the centrifugal tube, wherein the lysozyme amount is 0.120mg and 0.116mg respectively, and measuring that the average adsorbed protein amount of the glasses is 0.118mg after the glasses are worn for 1 day (8 hours), namely, the original protein amount of the glasses is considered to be 0.118 mg;

(2) the same person continuously wears the OK glasses with the same model to obtain two OK glasses with the surface provided with protein;

(3) soaking the OK lens in the step (2) in 1ml of 0.9% physiological saline for 3 minutes, then placing the OK lens into a 1.5ml centrifuge tube, adding 1ml of acetonitrile-trifluoroacetic acid solution into the centrifuge tube, vibrating for 30 minutes, and testing the lysozyme amount in the centrifuge tube to be 0.111 mg;

(4) placing the other OK lens in the step (2) in an electrophoresis dissociation bin of the hard corneal contact lens protein removal sterilization device, adding 1ml of 0.9% normal saline, starting the hard corneal contact lens protein removal sterilization device to clean for 3 minutes, wherein the voltage at two ends of an electrophoresis dissociation probe in cleaning is 4V, the current is 1mA (the voltage at two ends of the electrophoresis dissociation probe in cleaning can be 4V-5V, and the current is 1-4mA), placing the OK lens in a 1.5ml centrifuge tube after cleaning, adding 1ml of acetonitrile-trifluoroacetic acid solution into the centrifuge tube, and testing the lysozyme amount in the centrifuge tube to be 0.008mg after vibrating for 30 minutes;

(5) calculating the elution rate of the hard corneal contact lens protein removal and sterilization device matched with physiological saline on the surface protein of the OK lens:

similarly, the elution rate of proteins on the surface of the OK mirror soaked in the physiological saline is calculated to be equal to (1-0.111mg/0.118mg) × 100% >, 5.9%.

According to the experimental results, the protein removing and sterilizing device for the hard corneal contact lens can achieve 94.6% of surface protein elution rate of the Euclidean hard corneal plastic lens by being matched with physiological saline, and only 4.1% of protein elution rate can be achieved by soaking the Euclidean hard corneal plastic lens in the physiological saline, so that the protein removing and sterilizing device for the hard corneal contact lens can achieve the efficient cleaning and reducing effect of the Euclidean hard corneal plastic lens by being matched with the physiological saline, and the effect of the hard corneal contact lens removing and sterilizing device for the hard corneal contact lens, which is matched with the OK lens, can be verified.

2. Verify the situation that this application hard contact lens removes albumen sterilization apparatus and clears away the albumen on the RGP mirror:

the RGP mirror selected in the present verification example is an Abobodenamo pupil hard contact lens (day-wearing type RGP) (national mechanical Standard 20193160515, SN: DS200114121/DS200114122) material: hexafluoroisopropyl methacrylate, methacryloxypropyl tris (trimethylsiloxy) silane, methacrylic acid, methyl methacrylate, hydroxyethyl methacrylate, a crosslinking agent, an initiator, an ultraviolet absorber and a colorant; the electrolyte solution is selected as normal saline, namely 0.9% NaCl solution, and the specific method is as follows:

(1) after the human eyes wear the RGP lens for 8 hours, taking down the left and right lens, respectively putting the left and right lens into a 1.5ml centrifugal tube, adding 1ml acetonitrile-trifluoroacetic acid solution into the centrifugal tube, vibrating for 30 minutes, testing the lysozyme amount in the centrifugal tube, respectively being 0.215mg and 0.210mg, and measuring that the average adsorbed protein amount of the lens worn for 1 day (8 hours) is 0.212mg, namely considering that the original protein amount of the lens is 0.212 mg;

(2) the same person continuously wears the RGP mirror with the same model to obtain two RGP mirrors with protein on the surfaces;

(3) soaking the RGP lens in the step (2) in 1ml of 0.9% physiological saline for 3 minutes, then placing the RGP lens into a 1.5ml centrifuge tube, adding 1ml of acetonitrile-trifluoroacetic acid solution into the centrifuge tube, vibrating for 30 minutes, and testing the lysozyme amount in the centrifuge tube to be 0.201 mg;

(4) placing the other RGP lens in the step (2) in an electrophoresis dissociation bin of the rigid corneal contact lens deproteinization sterilization device, adding 1ml of 0.9% normal saline, starting the rigid corneal contact lens deproteinization sterilization device to clean for 3 minutes, wherein the voltage at two ends of an electrophoresis dissociation probe in cleaning is 4V, the current is 2mA (the voltage at two ends of the electrophoresis dissociation probe in cleaning can be 4V-5V, and the current is 1-4mA), placing the RGP lens in a 1.5ml centrifuge tube after cleaning is finished, adding 1ml of acetonitrile-trifluoroacetic acid solution into the centrifuge tube, and testing the quantity of lysozyme in the centrifuge tube to be 0.008mg after vibrating for 30 minutes;

(5) calculating the elution rate of the protein on the surface of the RGP lens by matching a rigid corneal contact lens protein removal and sterilization device with physiological saline:

similarly, the elution rate of proteins from RGP mirror surfaces soaked with saline was calculated to be equal to (1-0.201mg/0.212mg) × 100% >, 5.2%.

According to the experimental results, the surface protein elution rate of the rigid contact lens with the ebonodeprunox pupil can reach 96.2% by matching with physiological saline, and the protein elution rate of the rigid contact lens with the ebonodeprunox pupil soaked by the physiological saline is only 5.2%, so that the rigid contact lens protein removal and sterilization device can achieve the efficient cleaning and reduction effect by matching with the physiological saline on the rigid contact lens with the ebonodeprunox pupil, and the effect of the rigid contact lens protein removal and sterilization device on the RGP lens can be verified.

It should be noted that, in the experimental process of this verification example, the acetonitrile-trifluoroacetic acid solution is used to perform vibration extraction of protein, so that the protein attached to the lens can be separated from the lens, that is, the protein is dissolved in the protein extract, the light absorption value of the protein extract solution is detected by the micro ultraviolet spectrophotometer, and then the protein content is calculated according to the protein concentration detection standard curve.

The protein concentration detection standard curve needs to be artificially established, and the establishment of the protein concentration detection standard curve specifically comprises the following steps:

preparing a 4mg/ml protein solution, carrying out multiple dilution on the protein solution, testing the light absorption value and the protein concentration of the protein solution for multiple times, recording the average value of the light absorption value obtained by the test, recording the average value of the protein concentration obtained by the test, see the following table 3-1, making a standard curve according to the average value of the light absorption value and the average value of the protein concentration, and if the linear deviation degree exceeds the range of +/-10%, considering that the standard data are invalid and needing to be redone;

the function expression of the protein concentration detection standard curve is as follows:

y＝2.64033436x-0.001002579，

wherein y represents the measured protein concentration value, and x represents the light absorption value of the protein at 280 nm; performing a curve according to actual test dataFitting, degree of agreement between test data and fitting function, using a quantity R related to the correlation coefficient²To evaluate, R²The closer the value is to 1, the higher the degree of coincidence, the closer to 0, the lower the degree of coincidence, R being calculated in one case²＝0.999999835。

TABLE 3-1 dilution table for protein solutions by multiple ratio

Dilution factor	Measured concentration (mg/ml)	RSD	A280
					1	3.485	1％	9.20
2	1.702	0％	4.49
				4	0.857	0％	2.26
8	0.435	0％	1.15
				16	0.220	1％	0.58
32	0.118	3％	0.31
				64	0.056	2％	0.15

Verification example 4:

verify the effect of killing of this application rigid contact lens deproteinization sterilization apparatus to the bacterium:

the electrophoresis dissociation protein-removing function of the hard corneal contact lens protein-removing sterilization device is matched with 0.9% NaCl solution, and the liquid after electrophoresis dissociation for 3 minutes is subjected to sterilization detection, wherein the sterilization rate of escherichia coli, staphylococcus aureus and pseudomonas aeruginosa reaches 99.999%, and the sterilization rate of candida albicans reaches 99.99%.

The electrophoresis dissociation technology of the hard corneal contact lens protein removal and sterilization device can play a role in sterilization and disinfection by matching with an electrolyte solution, and further avoids the risk of bacterial infection of human eyes.

Verification example 5:

treating cysts containing Acanthamoeba (ATCC 30868Acanthamoeba castellani) and trophozoite PBS with a hard corneal contact lens protein removal and sterilization device, a standard working concentration disinfectant and normal physiological saline respectively, collecting a mixed solution, judging the survival rate of the polypide by morphological observation of the collected mixed solution through an iodine solution staining method, a hematoxylin staining method and the like, culturing an intraperitoneal inoculated mouse by applying an ATCC medium 712 culture medium, calculating the survival rates of the Acanthamoeba trophozoite and the cysts after the treatment of the hard corneal contact lens protein removal and sterilization device, the standard working concentration disinfectant and the normal physiological saline respectively, and comparing and verifying the survival rates with morphological observation results; finally, the test is repeated for 3 times, and the killing effect of the protein-removing and sterilizing device of the hard corneal contact lens, a standard disinfectant (corrosive and incapable of being sterilized by a conventional corneal contact tool) and normal saline (used as negative control) on the acanthamoeba is evaluated.

Preferably, molecular biological identification is carried out on the ATCC standard strain acanthamoeba, and the strain of the strain amebiasis is verified.

The experimental steps are as follows:

preparing an article: 5ml disposable empty needle, sterile bacteria culture flask, 15ml centrifuge tube, ATCC culture medium 712, hematoxylin-related staining solution, slide.

And (3) resuscitation:

(1) sterilizing the sterile operating table with ultraviolet rays for 20min, and adjusting the temperature of the water bath tank to 28 ℃;

(2) taking out the acanthamoeba freezing tube, and quickly melting in a water bath box;

(3) sucking the melted amoeba suspension by using an empty needle, adding the suspension into a centrifuge tube, sucking 2ml of ATCC culture medium 712 by using another empty needle, adding the ATCC culture medium into a freezing tube, blowing and cleaning the residual amoeba, and transferring the residual amoeba into the centrifuge tube;

(4) centrifuging at 500rpm for 7 minutes;

(5) adding 4ml of culture medium into a centrifuge tube, blowing and beating cells into suspension, dividing the suspension into two culture bottles, adding 5ml of culture medium into each bottle, and screwing a bottle cap;

(6) gently shaking the two culture bottles at 28 deg.C for 5min to disperse the aggregated cysts uniformly;

(7) performing horizontal incubation culture at 28 deg.C (sterile glove can be used for wrapping culture flask, sealing with adhesive tape, and preventing pollution);

(8) changing the liquid for 3-5 days, and carrying out passage after the nourishment body grows full.

Liquid changing or passage:

(1) lightly blowing and beating with a suction tube or an empty needle to suspend a small amount of trophozoite forced by iron (if only changing liquid, the step can be omitted);

(2) transferring the suspension into a centrifuge tube, and centrifuging for 7 minutes at 500 rpm;

(3) add fresh medium, blow to a suspension, and transfer to a culture flask.

In vitro observation of the effect of different treatments on acanthamoebae:

(1) acanthamoeba was subjected to three different treatments and to hematoxylin staining:

the acanthamoeba in the culture bottle is respectively treated by a rigid corneal contact lens protein removal and sterilization device (experimental group), a standard working concentration disinfectant (control group) and common normal saline (negative control), and the survival rate of the acanthamoeba is observed. The treated acanthamoeba is stained by hematoxylin staining by a physiological saline smear method, and the activity change of acanthamoeba trophozoite in a visual field is observed.

(2) Hematoxylin staining method

0.5 percent of sulindac solution, 4 percent of ferric alum solution and 2 percent of ferric alum solution and Shore fixing solution are prepared according to the conventional method.

Fixing, namely adding the cysts into Shore fixing liquid, fixing for 5 minutes at 40 ℃ or 20-30 minutes at room temperature, centrifuging (1500rpm) for 5 minutes, and removing supernatant.

③ 2 percent iodine tincture is added for 30 minutes to remove mercury, and the supernatant is removed by centrifugation.

Adding 70% alcohol, standing for 1 hour or overnight, removing mercury iodide, centrifuging to remove supernatant, adding water, standing for 10 minutes, and centrifuging to remove supernatant.

And fifthly, mordanting, adding 4 percent of iron alum solution, carrying out mordanting for 5 minutes at 40 ℃ in water bath or 30 minutes at room temperature, centrifuging to remove supernatant, and washing for 3 times by adding water.

Sixthly, after dyeing and centrifuging to remove the supernatant, adding 0.5 percent hematoxylin dye solution to dye in water bath at 40 ℃ for 5-15 minutes, centrifuging to remove the supernatant, and washing with water for 3 times.

Seventhly, after color separation and centrifugation are carried out to remove supernatant, 2% of iron alum solution is added to carry out color separation, the color fading condition of the capsule is observed under a microscope while color separation is carried out until the internal structure of the capsule is clear, and the capsule is generally washed for 3 times by adding water after 3-5 minutes.

After centrifuging to remove the supernatant, adding water to soak for 1 hour (preferably overnight) to remove the color separating agent, and centrifuging the supernatant.

Ninthly, adding 50-95% of alcohol and pure alcohol gradually for dehydration, dehydrating for 10 minutes at each stage, and centrifuging to remove supernatant.

Adding 10 parts of mixed solution of pure alcohol and wintergreen oil (or dimethylbenzene) to the transparent protein at the red spot, centrifuging to remove supernatant, adding 5 parts of pure wintergreen oil or dimethylbenzene solution, and centrifuging to remove supernatant.

Sealing, adding several drops of wintergreen oil or xylene solution (depending on the amount of precipitate), stirring, sucking 1/2 drops of encapsulated suspension with a small pipette, mixing with 1 drop of neutral gum, stirring, adding a cover slip, and air drying.

In vivo observations of the effect of different treatments on acanthamoebae:

the acanthamoeba cultures under different treatments were washed 3 times with PBS and the suspension was adjusted to 5ul PBS containing 1X 105 acanthamoeba trophozoites. Selecting female BALB/c mice with SPF grade of 6 weeks old, and respectively calculating the survival rates of acanthamoeba trophozoites and cysts under different treatment groups after the mice are subjected to intraperitoneal injection. If the experimental result proves that the protein removing and sterilizing device for the hard corneal contact lens has the effects of killing and inhibiting the acanthamoeba, the experiment is expected to be popularized to an animal model rabbit, and relevant experiments are further carried out on the rabbit cornea.

Verification example 6:

verifying the degreasing effect of the deproteinizing and sterilizing device for the hard corneal contact lens, the RGP lens is taken as an example in the verification example, the RGP lens is selected from an Epodermonian pupil hard contact lens (daily wearing type RGP), and the national mechanical standard 20193160515, SN: DS200355733/DS200355734, material quality: hexafluoroisopropyl methacrylate, methacryloxypropyl tris (trimethylsiloxy) silane, methacrylic acid, methyl methacrylate, hydroxyethyl methacrylate, a crosslinking agent, an initiator, an ultraviolet absorber and a coloring agent are polymerized to obtain the compound;

(1) preparing an artificial tear: phosphatidyl choline-0.0005 mg/ml, cholesterol-0.0018 mg/ml, lysozyme-1.9 mg/ml, BSA-0.2mg/ml, g-globulin-0.1 mg/ml, Nacl-9mg/ml, GaCL 2.2H2O-0.25 mg/ml, Na2HPO 4.7H2O-0.28 mg/ml, the pH is adjusted to 7.8 using NaOH or Hcl;

(2) manual adsorption of the pellet outside the RGP mirror: and (2) taking 2 pieces of the ebonodeprunox hard contact lens in a sterile environment, placing the ebonodeprunox hard contact lens in the artificial tear prepared in the step (1), immersing the contact lens in the artificial tear, and then placing the artificial tear in an incubator at 37 ℃ to replace the artificial tear every 24 hours. After 3 days of precipitate adsorption, the lenses were removed from the solution and tested after rinsing with saline.

(3) Fluorescence spectrophotometer test: exciting wavelength is 360nm, emission wavelength is 360nm, the RGP mirror adsorbed by the precipitate is placed in a fluorescence spectrophotometer, the center of the lens faces to the light beam, and the fluorescence intensity value of the emission spectrum is recorded.

(4) Washing the two lenses cultured in the step (2) by using physiological saline, placing the lenses in a fluorescence spectrophotometer for testing, and recording the fluorescence intensity value of an emission spectrum to obtain the fluorescence intensities of 29.6 and 28.9 before cleaning;

(5) placing the lens with the fluorescence intensity of 29.6 measured in the step (4) into an electrophoresis dissociation bin of the rigid contact lens deproteinization sterilization device, wherein the voltage at two ends of a probe in the electrophoresis dissociation bin is 4-5V, adding 1ml of 0.9% physiological saline, starting the device to clean for 30 minutes, taking out the lens, placing the lens into a fluorescence spectrophotometer to test, and recording the fluorescence intensity value of an emission spectrum to obtain the cleaned fluorescence intensity of 3.7;

(6) placing the lens with the fluorescence intensity of 28.9 measured in the step (4) into an electrophoresis dissociation bin of the rigid corneal contact lens deproteinization sterilization device, adding 1ml of 0.9% physiological saline, only standing and soaking for 30 minutes, taking out and placing in a fluorescence spectrophotometer for testing, and recording the fluorescence intensity value of an emission spectrum to obtain the cleaned fluorescence intensity of 27.3;

(7) calculating the fat removal rate of the rigid corneal contact lens protein removal sterilization device to the RGP lens in the step (5) as follows: (29.6-3.7)/29.6 ═ 87.5%;

and (3) calculating the degreasing rate of the lens soaked in the reduction instrument matched with the care solution in the step (6) as follows: (28.9-27.3)/28.9-5.5%.

According to the experimental results, the protein removing and sterilizing device for the hard contact lens has the advantages that the removal rate of grease on the surface of the ebonorops hard contact lens can reach 87.5%, and the removal rate of grease on the surface of the same lens, which is removed by soaking the same lens in the ebonorops hard contact lens in cooperation with physiological saline, is only 18.8%, so that the protein removing and sterilizing device for the hard contact lens has the effect of removing lipid under the condition of starting cleaning in cooperation with electrolyte liquid.

In summary, the rigid contact lens protein removing and sterilizing device and the method thereof can remove surface proteins, bacteria and lipid of the rigid contact lens by using electrolyte solution containing chloride ions, such as NaCl, so that the rigid contact lens is restored, and the safety of the lens entering the eye is guaranteed. Meanwhile, the protein removing and sterilizing device for the hard corneal contact lens is provided with the electricity storage device, is convenient to carry and can be normally used in environments without power supplies, such as the road and the like; the washing device is provided with a washing water tank and a liquid containing tank, and the cornea contact lens washed from the electrophoresis dissociation bin can be washed at any time and any place by matching with washing tools such as a watering can and the like, so that a user does not need to separately hold the whole device or the cornea contact lens to a water tank and other places for washing again, the washing convenience is greatly increased, and the defect that the cornea contact lens cannot be used due to the fact that no washing condition exists at the side is overcome; the special cleaning solution is sprayed to the corneal contact lens at each angle through flushing tools such as a watering can and the like, so that the lens can be prevented from being damaged or broken due to rubbing by hands; the liquid containing groove is detachably designed, and a notch which is convenient to remove the liquid containing groove is arranged between the liquid containing groove and the upper cover of the body, so that the washed waste liquid can be conveniently and conveniently poured out after the washing of the corneal contact lens is finished.

As used herein, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, including not only those elements listed, but also other elements not expressly listed.

In this document, the terms front, back, upper and lower are used to define the components in the drawings and the positions of the components relative to each other, and are used for clarity and convenience of the technical solution. It is to be understood that the use of the directional terms should not be taken to limit the scope of the claims.

The features of the embodiments and embodiments described herein above may be combined with each other without conflict.

The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.

Claims

1. A protein removing and sterilizing device for a hard corneal contact lens is characterized by comprising:

the cleaning device comprises a body, a cleaning device and a cleaning device, wherein an accommodating cavity is formed in the body, an electricity storage device, a circuit board and a switch assembly are accommodated in the accommodating cavity, the electricity storage device and the switch assembly are respectively and electrically connected with the circuit board, and a cleaning water tank is arranged on one side of the body;

the electrophoresis separates the storehouse, the electrophoresis separates the storehouse and locates the body is equipped with the one side of wasing the basin, at least, be provided with a set of electrophoresis in the electrophoresis separates the storehouse and separate the probe, the electrophoresis separates the storehouse intussuseption and is filled with and contains Cl^-The electrophoretic dissociation probe and the Cl-containing compound^-The electrophoretic dissociation probe is electrically connected with the circuit board; and

the liquid containing groove is arranged on the other side of the body, and the position of the liquid containing groove corresponds to the position of the washing water tank.

2. The apparatus for deproteinizing and sterilizing a hard corneal contact lens as defined in claim 1, wherein the cleaning water tank is a hollow design, the opening of the liquid tank faces the cleaning water tank, the cleaning water tank is capable of facilitating the washing of the hard corneal contact lens after the deproteinizing and sterilizing in the electrophoresis dissociation chamber, and the washed washing liquid can flow into the liquid tank through the hole in the cleaning water tank.

3. The rigid contact lens deproteinizing sterilization apparatus as claimed in claim 2, wherein the body comprises a body top cover and a body base, the liquid container is located at one side of the body base, the liquid container and the body base are separated, the body top cover is clamped on the body base and the liquid container, the cleaning water container is located at one side of the body top cover away from the liquid container, and the liquid container can be removed from the body.

4. The rigid corneal contact lens deproteinizing sterilization device as recited in claim 3, wherein the connection between the liquid tank and the body is a magnetic adsorption structure, a movable slot structure, or a buckle structure;

one side of the upper cover of the body is provided with at least one notch which is convenient for removing the liquid containing groove.

5. The protein removing and sterilizing device for the hard corneal contact lens as claimed in claim 1, wherein the liquid holding tank extends beyond the area of the cleaning water tank in the length direction and/or the width direction to expand the capacity of the liquid holding tank.

6. The apparatus for deproteinizing and sterilizing a hard corneal contact lens as claimed in claim 3, wherein an annular protrusion is provided at an opening of the cleaning water tank on the upper cover of the body;

the opening of the cleaning water tank is clamped with a water tank sealing cover.

7. The rigid contact lens deproteinizing and sterilizing device according to claim 3, wherein a mounting groove is formed in one side of the upper cover of the body, which is away from the base of the body, the electrophoresis dissociation chamber is mounted in the mounting groove in a magnetic adsorption manner or a buckling manner, an adapting pogo pin is disposed in the mounting groove at a position corresponding to the electrophoresis dissociation probe, the adapting pogo pin is electrically connected to the circuit board, and when the electrophoresis dissociation chamber is mounted in the mounting groove, the adapting pogo pin is connected to the corresponding electrophoresis dissociation probe.

8. The apparatus for deproteinizing and sterilizing a hard contact lens according to claim 1, wherein each set of electrophoresis dissociation probes in the electrophoresis dissociation chamber comprises at least a first probe and a second probe, the first probe and the second probe are arranged at an interval, and the hard contact lens can be placed between the first probe and the second probe.

9. The rigid contact lens deproteinizing and sterilizing device according to claim 8, wherein the rigid contact lens and the electrolyte solution are placed in the electrophoresis dissociation chamber, the rigid contact lens deproteinizing and sterilizing device is started, the first probe and the second probe in the electrophoresis dissociation chamber form a positive probe and a negative probe under a circuit loop, the protein attached to the surface of the rigid contact lens to be cleaned and the oxygen permeation hole is charged in the electrolyte solution, and the charged tear protein moves towards the position of the electrophoresis dissociation probe opposite to the electric property of the charged tear protein;

10. The rigid corneal contact lens deproteinizing and sterilizing apparatus as claimed in claim 9, wherein the electrolyte solution is a NaCl-containing solution,

the hypochlorous acid and the protein are subjected to redox reaction in the electrophoretic separation bin, and the protein is degraded, and the method specifically comprises the following steps:

11. A method for deproteinizing and sterilizing a hard corneal contact lens is characterized by comprising the following steps:

adding Cl-containing solution into the electrophoresis dissociation chamber of the rigid cornea contact lens deproteinization sterilization device^-The electrolyte solution and the hard contact lens to be cleaned are mixed, and a switch of the protein removing and sterilizing device of the hard contact lens is opened to remove the protein and sterilize the hard contact lens;

after the rigid corneal contact lens protein removal and sterilization device works for a set time, putting the cleaned rigid corneal contact lens into a cleaning water tank;

flushing the hard corneal contact lens in the cleaning water tank by using a flushing tool until the electrolyte solution on the hard corneal contact lens is completely flushed;

and the washing liquid flows into the liquid containing groove through the hollow part of the washing water groove.

12. The method for deproteinizing and sterilizing a hard corneal contact lens as recited in claim 11, wherein when a switch of the device for deproteinizing and sterilizing a hard corneal contact lens is turned on, the first probe and the second probe in the electrophoresis dissociation chamber form a positive probe and a negative probe under a circuit loop, the lacrimal proteins attached to the surface of the hard corneal contact lens to be cleaned and the oxygen permeation pores are charged in an electrolyte solution, and the charged lacrimal proteins move towards the position of the probe for electrophoresis dissociation which is opposite to the charged lacrimal proteins;

cl in the electrolyte solution^-Moving towards the positive probe, oxidizing the lost electrons into chlorine, dissolving the chlorine in the electrolyte solution to generate hypochlorous acid, carrying out redox reaction on the hypochlorous acid and the lachrymarin in the electrophoretic separation bin, and degrading the lachrymarin;

13. The method for deproteinizing and sterilizing a hard corneal contact lens as claimed in claim 12, wherein the electrolyte solution is a solution containing NaCl, the hydrogen ions with positive charges in the electrolyte solution move to the negative probe and undergo a reduction reaction near the negative probe to generate hydrogen gas, the sodium ions in the electrolyte solution and the hydroxide ions generate sodium hydroxide, the grease on the surface of the hard corneal contact lens undergoes a saponification reaction with the sodium hydroxide, and the grease on the surface of the hard corneal contact lens is removed.