CN114642258A - Beverage composition for relieving stress, relaxing mood and improving sleep of young people and preparation method thereof - Google Patents

Beverage composition for relieving stress, relaxing mood and improving sleep of young people and preparation method thereof Download PDF

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CN114642258A
CN114642258A CN202210262395.7A CN202210262395A CN114642258A CN 114642258 A CN114642258 A CN 114642258A CN 202210262395 A CN202210262395 A CN 202210262395A CN 114642258 A CN114642258 A CN 114642258A
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weight
parts
powder
beverage composition
sleep
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张连龙
王继武
毛兆祥
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Wuxi Jiante Pharmaceutical Co ltd
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
    • A23L19/01—Instant products; Powders; Flakes; Granules
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
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    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/175—Amino acids
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
    • A61K31/19—Carboxylic acids, e.g. valproic acid
    • A61K31/195—Carboxylic acids, e.g. valproic acid having an amino group
    • A61K31/197—Carboxylic acids, e.g. valproic acid having an amino group the amino and the carboxyl groups being attached to the same acyclic carbon chain, e.g. gamma-aminobutyric acid [GABA], beta-alanine, epsilon-aminocaproic acid, pantothenic acid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
    • A61K31/19—Carboxylic acids, e.g. valproic acid
    • A61K31/195—Carboxylic acids, e.g. valproic acid having an amino group
    • A61K31/197—Carboxylic acids, e.g. valproic acid having an amino group the amino and the carboxyl groups being attached to the same acyclic carbon chain, e.g. gamma-aminobutyric acid [GABA], beta-alanine, epsilon-aminocaproic acid, pantothenic acid
    • A61K31/198—Alpha-aminoacids, e.g. alanine, edetic acids [EDTA]
    • A—HUMAN NECESSITIES
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/702—Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/35—Caprifoliaceae (Honeysuckle family)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/72—Rhamnaceae (Buckthorn family), e.g. buckthorn, chewstick or umbrella-tree
    • A61K36/725—Ziziphus, e.g. jujube
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
    • A61K36/736—Prunus, e.g. plum, cherry, peach, apricot or almond
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/20—Hypnotics; Sedatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

Abstract

The invention relates to a beverage composition for alleviating pressure, relaxing mood and improving sleep of young people and a preparation method thereof. The beverage composition comprises gamma-aminobutyric acid, theanine, wild jujube kernel powder, xylooligosaccharide, sour cherry concentrated powder, balm powder, German chamomile extract, passion flower powder, elderberry juice powder and the like. According to the invention, through food with homology of medicine and food, the level of inhibitory neurotransmitter gamma-aminobutyric acid is up-regulated, and the level of excitatory neurotransmitter glutamic acid is down-regulated, so that the gamma-aminobutyric acid and the glutamic acid are balanced, the effects of relieving stress, anxiety and depression of young people, antagonizing excitation of caffeine and finally improving sleep are achieved. The zebra fish and human body tests prove that the medicine has remarkable effect and is worth popularizing in young insomnia people.

Description

Beverage composition for relieving stress, relaxing mood and improving sleep of young people and preparation method thereof
[ technical field ] A method for producing a semiconductor device
The invention belongs to the technical field of functional foods. More particularly, the invention relates to a drink composition for alleviating stress, relaxing mood and improving sleep of young people, and also relates to a preparation method of the drink composition.
[ background of the invention ]
According to the World Health Organization (WHO) statistics: about 27% of all people worldwide have sleep problems, i.e. 1 out of every 4 people sleep poorly.
According to the research data result of the Jingdong research platform, more than 7 users show that the users have sleep problems with different degrees and different expression forms. 66% of people have insufficient sleeping time, 61% of people have irregular work and rest time, 51% of people are often troubled by sleeping problems such as difficulty in falling asleep, getting up at night, snoring and the like, and 45% of people have serious influence on work and living conditions. The insomnia people tend to be younger, wherein the ages of insomnia people close to 8 are below 35, the ages of insomnia people 18-25 are 39.52%, the ages of insomnia people 26-30 are 23.14%, the ages of insomnia people 26-30 are 16.07%, the ages of insomnia people 35-40 are 8.57%, the ages of insomnia people 41-50 are 10.01%, and the ages of insomnia people above 51 are 2.69%, and most of insomnia reasons are caused by emotional disturbance and working pressure; the largest psychological factor affecting insomnia is stress anxiety dysphoria. The Jingdong '2019-2020 on-line sleep consumption report' shows that the sleep problems after 90 and 80 are more serious than those of the middle-aged and the elderly, and the sleep problems after 90 are serious disaster areas.
The insomnia of the old and the young have the following differences:
youth (18-44 years old) Middle aged and elderly people (over 45 years old)
Physiology of human body Equilibrium of synthesis and decomposition Decomposition over synthesis
Characteristics of Active insomnia Passive insomnia
Reason Stress, anxiety, depression, drinking coffee, playing mobile phone Biological clock disorders
At present, western medicines have certain side effects on insomnia treatment, patients can have drug dependence and drug addiction, the dosage of the patients is gradually increased along with the increase of time, and the insomnia continues after the medicine is stopped. The traditional Chinese medicine treatment makes a certain progress in the research of treating insomnia, and most of the existing traditional Chinese medicine compositions have numerous raw materials, poor taste, unclear mechanism and poor effect.
Therefore, the present inventors have completed the present invention by summarizing the prior art through a large number of experimental studies and analyses based on the technical defects of the prior art.
[ summary of the invention ]
[ problem to be solved ]
The invention aims to provide a beverage composition for alleviating stress, relaxing mood and improving sleep of young people.
Another object of the present invention is to provide a method for preparing said drink composition.
[ solution ]
The invention is realized by the following technical scheme.
The invention relates to a beverage composition for alleviating pressure, relaxing mood and improving sleep of young people.
The beverage composition comprises the following components: in parts by weight
Figure BDA0003551010140000021
The total amount of water is 100 parts by weight.
According to a preferred embodiment of the present invention, the composition of the drink composition is as follows: in parts by weight
Figure BDA0003551010140000022
Figure BDA0003551010140000031
The total amount of water is 100 parts by weight.
According to another preferred embodiment of the invention, the composition of the drink composition is as follows: in parts by weight
Figure BDA0003551010140000032
The total amount of water is 100 parts by weight.
According to another preferred embodiment of the invention, the drink composition contains 0.001 to 25.0 parts by weight of sweetener.
According to another preferred embodiment of the present invention, the sweetener is one or more selected from the group consisting of white granulated sugar, erythritol, xylitol, isomalt, isomaltooligosaccharide, sucralose, acesulfame potassium, stevia, and glycyrrhizin.
According to another preferred embodiment of the invention, the drink composition contains 0.001-1 part by weight of an acid-base regulator.
According to another preferred embodiment of the present invention, the pH regulator is one or more pH regulators selected from malic acid, citric acid, sodium citrate, lactic acid and fumaric acid.
The invention relates to a preparation method of a beverage composition for alleviating pressure, relaxing mood and improving sleep of young people.
The preparation method comprises the following steps:
firstly, 0.08-4 parts by weight of sour cherry concentrated powder, 0.02-2.0 parts by weight of gamma-aminobutyric acid, 0.016-1.6 parts by weight of tea theanine, 0.02-2.0 parts by weight of wild jujube kernel powder, 0.12-12.0 parts by weight of xylo-oligosaccharide, 0.01-1.0 part by weight of passion fruit powder and 0.05-5.0 parts by weight of elderberry juice powder are uniformly stirred, then 0.001-1.0 part by weight of acid-base regulator and 0.001-25.0 parts by weight of sweetening agent are added, then the raw materials are added into 50 parts by weight of water and heated and dissolved at the temperature of 80 ℃, the obtained solution is filtered by using a plate-frame filter, and filtrate is collected;
secondly, adding 0.01 to 1.6 weight parts of balm bee powder, 0.01 to 1.0 weight part of German chamomile extract and 0.001 to 1.0 weight part of edible essence into the filtrate, uniformly stirring, and adding water until the total weight is 100 weight parts; and then cooling the temperature of the beverage to 45 ℃, filtering the beverage by using a plate-and-frame filter, collecting filtrate, filling the filtrate into a clean bottle, sealing the bottle, placing the bottle in a water bath sterilization cabinet, and sterilizing the bottle at the temperature of 105-115 ℃ for 20 minutes to obtain the beverage composition.
According to a preferred embodiment of the invention, the collected filtrate is placed in an ultrahigh-temperature instant sterilizer for sterilization, the sterilized filtrate is then put into a clean bottle and covered, and the beverage composition is obtained by passing through a conveyor belt, a spray cooler, hot water spraying at 80-90 ℃ and warm water cooling at 25-40 ℃.
According to another preferred embodiment of the present invention, the pH regulator is one or more pH regulators selected from malic acid, citric acid, sodium citrate, lactic acid, and fumaric acid; the sweetener is one or more selected from white sugar, erythritol, xylitol, isomalt, isomaltose hypgather, sucralose, acesulfame potassium, stevioside or glycyrrhizin.
The present invention will be described in more detail below.
The cause of insomnia in young people is the imbalance in the proportion of neurotransmitters (neurotranssmitter) caused by stress, anxiety, depression, coffee, cell phone play, etc. Neurotransmitters are chemical substances that transmit information between neurons or between neurons and effector cells such as muscle cells, glandular cells, etc. One neurotransmitter is an excitatory neurotransmitter and the other neurotransmitter is an inhibitory neurotransmitter. The quality of sleep is not in the number of neurotransmitters but in the ratio of the two neurotransmitters. Excitatory neurotransmitters are mainly glutamate, and inhibitory neurotransmitters are mainly gamma-aminobutyric acid. According to the invention, gamma-aminobutyric acid is up-regulated and glutamic acid is down-regulated through food with homology of medicine and food, so that the gamma-aminobutyric acid and the glutamic acid are balanced, the effects of relieving stress, anxiety and depression of young people and antagonizing caffeine excitation are achieved, and the effect of improving sleep is finally achieved.
The invention relates to a beverage composition for alleviating pressure, relaxing mood and improving sleep of young people.
The drink composition comprises the following components: in parts by weight
Figure BDA0003551010140000041
The total amount of water is 100 parts by weight.
Among them, gamma-aminobutyric acid (GABA), an inhibitory neurotransmitter widely present in the central nervous system, is produced from the excitatory neurotransmitter glutamate through decarboxylation under the catalysis of glutamate decarboxylase, and binds to GABA receptors of postsynaptic membranes, resulting in the opening of ion channels, the influx of negatively charged chloride ions into cells, and the efflux of positively charged potassium ions out of cells, and the inhibition of central neurons through the slowing of the rate of postsynaptic neuron discharge, thus achieving the effect of improving sleep. After exogenous GABA is absorbed through intestinal tracts, GABA can rapidly enter blood, is combined with GABA receptors on cells to play a role in inhibiting sympathetic nerves, can improve activity of parasympathetic nerves, and achieves the effects of improving alpha waves, inhibiting beta waves and relieving pressure, and the method is specifically disclosed in the following documents: congratulatory et al, entitled "neurotransmitter research for insomnia", journal of neurology and neurorehabilitation, 2011, 8(1), pp51-53 "; lei Ke et al, entitled "research progress of mechanism of action of gamma-aminobutyric acid to improve sleep", "food industry science and technology", 2019, 40(14), pp 353-358.
Gamma-aminobutyric acid was approved as a new resource food in 2009, and the specifications thereof are listed in table 1 below.
Table 1: gamma-aminobutyric acid as new resource food
Figure BDA0003551010140000051
According to the present invention, the main effects of gamma-aminobutyric acid in the beverage composition for stress relief, mood relaxation and sleep improvement of young people according to the present invention are to up-regulate gamma-aminobutyric acid level, relieve stress, relax mood and improve sleep.
The gamma-aminobutyric acid used in the present invention is a product currently marketed, for example, gamma-aminobutyric acid marketed by Nanjing Jenno Biotechnology, Inc.
Glutamic acid (Glu) is a main excitatory neurotransmitter in brain, has strong excitatory action on neurons such as cerebral cortex, hypothalamus, hippocampus and the like, and excessive Glu generates excitatory neurotoxicity on the neurons and plays an important role in insomnia. The tea component L-theanine has a chemical structure similar to that of glutamic acid, can be absorbed by small intestine after being taken orally, can directly pass through a blood brain barrier to play a role in the brain, and compete for a glutamic acid receptor, so that the glutamic acid level is reduced. Experimental studies prove that theanine has the effects of improving sleep, promoting the generation of alpha waves and antagonizing caffeine excitation, and specifically refer to the following documents: zhang Ying et al, entitled "research on sleep improving effect of L-theanine", science and technology in food industry, 2021, 42(16), pp 361-366.
Tea theanine was approved as a new source food in 2014 and its specifications are listed in table 2 below.
Table 2: new resource food theanine
Figure BDA0003551010140000061
Modern researches prove that gamma-aminobutyric acid and tea theanine have a synergistic effect, so that the sleep time is shortened and prolonged, and the specific references are as follows: suhyeon kim et al, "GABA and L-the amine mixed defects sleep latency and improves NREM sleep", PHARMACEUTICAL BIOLOGY, 2019, 57(1), pp64-72 ".
According to the invention, the main function of theanine in the beverage composition for alleviating stress, relaxing mood and improving sleep of young people is to compete for glutamate receptors and reduce glutamate level, thereby promoting the generation of alpha wave, antagonizing caffeine excitation and improving sleep.
Theanine used in the present invention is a product currently marketed, for example, theanine sold by shanghai nodd biologicals ltd, wuxi century biologicals ltd.
The xylo-oligosaccharide is obtained by enzymolysis of wheat straw serving as a raw material by xylanase by a steam explosion method. It consists of xylobiose-xyloheptaose. Exogenously supplying xylooligosaccharide, proliferating intestinal lactobacillus plantarum and lactobacillus brevis, stimulating intestinal epithelial cells through metabolism of the lactobacillus plantarum and the lactobacillus brevis, and secreting and inhibiting neurotransmitter gamma-aminobutyric acid. The functions of relieving stress anxiety and relaxing mood are achieved by the joint action of the intestinal and brain axes consisting of the endocrine nervous system, the immune nervous system and the vagus nervous system. See in particular the literature: zhang Qiuxiang et al, entitled "research on utilization of fructo-oligosaccharide and xylo-oligosaccharide by lactic acid bacteria", journal of food and biotechnology 2020, 39(4), pp18-23, Shishuyan et al, entitled "research on interaction of nutrients and genes mediated by intestinal flora in sleep regulation", journal of Chinese micro ecology, 2017, 29(11), pp 1333-1338.
Xylo-oligosaccharides were approved as a new source food in 2014, and the specifications thereof are listed in table 3 below.
Table 3: new resource food xylo-oligosaccharide
Figure BDA0003551010140000071
Figure BDA0003551010140000081
According to the invention, the main effects of xylo-oligosaccharide in the drink composition for alleviating stress, relaxing mood and improving sleep of young people are to proliferate lactobacillus plantarum and lactobacillus brevis, up-regulate gamma-aminobutyric acid level and play a role in improving sleep through intestinal and cerebral axes.
The xylo-oligosaccharide used in the present invention is a product currently on the market, for example, xylo-oligosaccharide sold by Shandong Longli Biotechnology Ltd.
The sour cherry concentrated powder is a special European sour cherry variety, contains rich plant active ingredients, namely anthocyanin, procyanidine, chlorogenic acid and melatonin, and natural plant prebiotics, can proliferate intestinal beneficial flora, optimize intestinal-cerebral axis, inhibit degradation of body tryptophan, and enhance synthesis of body endogenous sleep nutrient substances by taking the intestinal tract as a target spot.
According to the invention, the concentrated sour cherry powder has the main effects of containing natural melatonin and natural plant prebiotics, up-regulating gamma-aminobutyric acid receptors and playing a role in improving sleep in the drink composition for relieving pressure, relaxing mood and improving sleep of young people. See in particular the literature: the subjects of Si Rui, sour cherry juice is helpful to improve sleep, Chinese fruit industry information, 2014, 31(2), p66, Yijian and the like, and the subjects of Chinese research progress of some nutrients, food and medicinal and edible plants influencing sleep, food science 2015, 36(11) and pp 261-266.
The sour cherry concentrated powder used in the present invention is a product currently sold on the market, for example, a sour cherry concentrated powder sold by Shanghai Chengnian health science and technology Limited.
Melissa officinalis is a perennial herbaceous plant native to europe, zhoyao and iran. In the middle ages, artificial planting began and widely used in europe, and is now distributed all over the world. The melissa officinalis essential oil is a liquid gold and has a very good nerve soothing effect. Melissa officinalis in pharmacopoeia and traditional medicine for the treatment of gastrointestinal disorders and for the treatment of sleep disorders; can be used as sedative, spasmolytic and antibacterial agent in folk medicine. Melissa powder is a potent inhibitor of GABA transaminase (GABA-T). Modern researches prove that the melissa powder can improve the sleep quality of patients with chronic heart failure and regulate the emotion and cognitive ability. See in particular the literature: sword et al, entitled "research progress of some nutrients, foods and plants for medicine and food affecting sleep in China", food science 2015, 36(11), pp 261-266.
According to the present invention, the main effect of the wasp powder in the beverage composition for young people to relieve stress, relax mood and improve sleep is that the wasp powder is an effective inhibitor of GABA transaminase (GABA-T) and reduces the metabolism of GABA.
The melissa powder used in the present invention is a product currently marketed, for example, melissa powder sold by tokyo jinoco biotechnology limited.
Passion flower is a kind of vine plant of Passion genus, native to North south America and West Indian Islands, and has white, blue or purple, pink flower and edible fruit. Passion flower contains flavonoid compounds, indole alkaloids and other active substances. Passiflora edulis powder enhances affinity to GABA receptors and inhibits reuptake of GABA by rat cortical synaptosomes. Modern researches prove that the passion flower powder can improve the sleep quality. See in particular the literature: houxin Juan et al, entitled "research on Pink Passiflora Caerulea literature and discussion on Chinese medicinal properties", journal of Chinese materia medica 2021, 46(8), pp 1943-1950.
According to the present invention, the main effects of passion flower powder in the beverage composition for alleviating stress, relaxing mood and improving sleep of young people are to enhance the affinity to GABA receptors and reduce the reuptake of GABA.
The passion flower powder used in the present invention is a product currently on the market, such as the passion flower powder sold by Nanjing Jenno Biotechnology, Inc.
Chamomile (german) extract (also called chamomile german) dates back to ancient egypt, and romans like to use it as a beverage and a spice, which has the function of relieving emotions. The German chamomile extract is mainly used as a sedative, an anxiolytic and a spasmolytic. A total of about 120 secondary metabolites were detected in dried German chamomile flowers, including 28 terpenoids and 36 flavonoids. Terpenoids include alpha-bisabolol and its oxides, azulenes, sesquiterpenes, coumarins and umbelliferones; the flavonoids are mainly apigenin, azulene, farnesene, marigold, quercetin, luteolin, etc., and other components including ethers, tannin, chamomilic acid, choline, polysaccharide, phytoestrogen, etc. Apigenin is present in chamomile in high amounts (840 mg/100 g), but very little is present in free form, mainly in the form of various glycosides. Apigenin has effect similar to benzodiazepine, and can bind with benzodiazepine receptor to enhance GABA receptor activity. Modern researches prove that the German chamomile extract can improve the sleep quality of old people, improve the sleep quality of postpartum sleep disorder women and relieve anxiety. See in particular the literature: wu Ling Feng et al, entitled "preliminary study on sleep-aiding effect of natural fragrance of four aromatic plants", Shanghai agricultural science and technology, 2011, 4, pp 22-24.
According to the present invention, the major effects of the german chamomile extract in the drink composition for relieving stress, relaxing mood and improving sleep of young people according to the present invention are to enhance the GABA receptor activity and to up-regulate GABA levels.
The extract of German yellow chrysanthemum used in the present invention is a product currently marketed, for example, the extract of German yellow chrysanthemum marketed by Jiang Biotech, Shaanxi.
Elderberry is the berry of elderberry of Caprifoliaceae, is red or purple, and has a long history of eating in Europe and America. The elderberry contains cyanidin-3-glucoside, cyanidin-3-mulberry glucoside-5-glucoside, pelargonium-3-glucoside, cyanidin-3-rutinoside and other anthocyanins, and has strong free radical scavenging capacity and antioxidation; it contains polyphenol compounds such as quercetin, and has sleep improving effect; contains multiple nutrients such as magnesium, vitamins A, B1, B2, B6, C and 16 amino acids, and has the function of enhancing immunity. See in particular the literature: red plum, entitled "research on chemical components, pharmacological activity and edible value of elderberry", ginseng research 2006, 4, pp 23-26.
According to the invention, the elderberry juice powder has the main effects of activating GABA receptors by quercetin in the beverage composition for alleviating pressure, relaxing mood and improving sleep of young people, improving sleep-wake cycle, containing rich vitamins and enhancing the immunity of insomnia people.
The elderberry juice powder used in the present invention is a product currently sold on the market, for example, the elderberry juice powder sold by yota international (incorporated by reference).
The spina date seed is a dry and mature seed of the wild jujube, has the effects of nourishing the heart and tonifying the liver, calming the heart and tranquilizing the mind, arresting sweating, promoting the production of body fluid and the like, and is commonly used for treating dysphoria, insomnia, palpitation due to fright, dreaminess, body deficiency and hyperhidrosis, body fluid deficiency and thirst and the like. The substance basis and the action mechanism of the spina date seed for treating the insomnia achieve the effect of treating the insomnia by acting components such as quercetin, ursolic acid, palmitic acid, linoleic acid, oleanolic acid and the like on signal paths such as TNF signal paths, dopaminergic synapses, serotonergic synapses, GABA synapses, NF-kappa B and the like. According to the topological analysis of the active component-target point-channel interaction network diagram of the spina date seed, the components with the highest two values and the highest intermediate center value are quercetin, Kambe et al researches the adjusting influence of quercetin on sleep-arousal, finds that the rapid eye movement sleep can be remarkably reduced by injecting quercetin into the abdominal cavity of a normal rat, and the rapid eye movement sleep reduction caused by the quercetin is blocked by injecting a GABA receptor antagonist into the brain, and shows that the quercetin changes the sleep-arousal by activating a GABA receptor. Singeun et al conducted sleep-promoting studies of quercetin-related glycosides using ICR and SD rats, and also found that quercetin has a potential effect on GABA receptors to further improve sleep. See in particular the literature: li jin Jing et al, entitled "comparative study of anti-insomnia action mechanism of semen Ziziphi Spinosae and fructus Ziziphi Spinosae pulp based on network pharmacology", modern Chinese medicine 2021.
According to the invention, the main effect of the spina date seed in the drink composition for relieving pressure, relaxing mood and improving sleep of young people is to achieve the effect of treating insomnia by regulating the GABA content of cerebral cortex of an insomnia patient and the expression of GABA receptor mRNA.
The spina date seed used in the invention is a product sold in the market at present, for example, the spina date seed sold by Jiang Ha Biotechnology GmbH in Shaanxi.
According to the invention, the main function of the edible essence in the beverage composition for alleviating stress, relaxing mood and improving sleep of young people is to regulate the odor of the beverage.
The edible essences used in the present invention are cranberry essence, rose essence, lemon essence, and elderberry essence, which are all products currently sold in the market, such as cranberry essence sold by Guangzhou Kai essence perfumery Co., Ltd, rose essence sold by Senxin essence pigment technology (China) Co., Ltd, lemon essence sold by Hongtai Biotechnology Co., Ltd, and elderberry essence sold by Youda International (Gmby).
According to the invention, when the contents of theanine, xylo-oligosaccharide, sour cherry concentrated powder, wild jujube kernel powder, passion flower powder, elderberry juice powder, balm grass powder, German chamomile extract and edible essence are in the range, if the content of gamma-aminobutyric acid is lower than 0.02 part by weight, the effects of relieving pressure, relaxing mood and improving sleep are not obvious; if the content is more than 2.0 parts by weight, the new resource food regulation is not met; therefore, the content of γ -aminobutyric acid is suitably 0.02 to 2.0 parts by weight, preferably 0.2 to 2.0 parts by weight, more preferably 0.3 to 1.0 part by weight;
similarly, when the content of gamma-aminobutyric acid, xylo-oligosaccharide, sour cherry concentrated powder, wild jujube kernel powder, passion fruit powder, elderberry juice powder, balm meadow, German chamomile extract and edible essence is in the range, if the content of theanine in tea leaves is lower than 0.016 parts by weight, the effects of relieving pressure, relaxing mood and improving sleep are not obvious; if its content is more than 1.6 parts by weight, it does not meet the new resource food regulation; therefore, the theanine content in the tea leaves is reasonably 0.016 to 1.6 parts by weight, preferably 0.04 to 1.6 parts by weight, more preferably 0.06 to 0.8 parts by weight;
when the content of the gamma-aminobutyric acid, the theanine of tea leaves, the concentrated powder of sour cherry, the powder of wild jujube kernel, the passion flower powder, the powder of elderberry juice, the powder of balm beefwood, the extract of German chamomile and the edible essence is in the range, if the content of the xylo-oligosaccharide is lower than 0.12 part by weight, the effects of relieving pressure, relaxing mood and improving sleep are not obvious; if it is more than 12.0 parts by weight, it does not meet the new resource food regulations; therefore, the content of xylooligosaccharide is suitably 0.12 to 12.0 parts by weight, preferably 2.0 to 4.8 parts by weight, more preferably 3.0 to 4.4 parts by weight;
when the content of gamma-aminobutyric acid, theanine, xylo-oligosaccharide, spina date seed powder, passion flower powder, elderberry juice powder, bald bee powder, German chamomile extract and edible essence is in the range, if the content of the sour cherry concentrated powder is lower than 0.08 part by weight, the effects of relieving pressure, relaxing mood and improving sleep are not obvious; if it is more than 4 parts by weight, the beverage tastes bad; therefore, the content of the sour cherry concentrated powder is preferably 0.08 to 4.0 parts by weight, preferably 0.4 to 4.0 parts by weight, more preferably 0.6 to 2.0 parts by weight;
when the content of the gamma-aminobutyric acid, the theanine of tea leaves, the xylo-oligosaccharide, the concentrated powder of sour cherry, the powder of spine date seed, the powder of elderberry juice, the powder of balm grass, the extract of German chamomile and the edible essence is in the range, if the content of the passion flower powder is lower than 0.01 part by weight, the effects of relieving pressure, relaxing mood and improving sleep are not obvious; if it is more than 1.0 part by weight, the taste of the beverage is not good; therefore, it is feasible that the passion flower powder is contained in an amount of 0.01 to 1.0 part by weight, preferably 0.02 to 1.0 part by weight, more preferably 0.03 to 0.8 part by weight;
when the content of the gamma-aminobutyric acid, the theanine of tea leaves, the xylo-oligosaccharide, the concentrated powder of sour cherry, the passion flower powder, the elderberry juice powder, the balm powder, the German chamomile extract and the edible essence is within the range, if the content of the spine date seed powder is lower than 0.02 part by weight, the effects of relieving pressure, relaxing mood and improving sleep are not obvious; if the content is more than 2.0 parts by weight, the beverage is precipitated and has poor sense; therefore, the content of the wild jujube kernel powder is suitably 0.02 to 2.0 parts by weight, preferably 0.2 to 2.0 parts by weight, more preferably 0.2 to 1.5 parts by weight;
when the content of gamma-aminobutyric acid, theanine, xylo-oligosaccharide, concentrated sour cherry powder, passion flower powder, spina date seed powder, balm grass powder, German chamomile extract and edible essence is in the range, if the content of the elderberry juice powder is less than 0.05 part by weight, the effects of relieving pressure, relaxing mood and improving sleep are not obvious; if its content is more than 5.0 parts by weight, the cost of the beverage is too high; therefore, the content of the elderberry juice powder is suitably 0.05 to 5.0 parts by weight, preferably 0.5 to 5.0 parts by weight, more preferably 0.6 to 3.0 parts by weight;
when the content of gamma-aminobutyric acid, theanine, xylo-oligosaccharide, concentrated sour cherry powder, passion flower powder, spina date seed powder, elderberry juice powder, German chamomile extract and edible essence is in the range, if the content of the melissa powder is lower than 0.01 part by weight, the effects of relieving pressure, relaxing mood and improving sleep are not obvious; if it is more than 1.6 parts by weight, the taste of the beverage is not good; therefore, the content of the melissa powder is suitably 0.01 to 1.6 parts by weight, preferably 0.02 to 1.0 part by weight, more preferably 0.03 to 0.8 part by weight;
when the content of the gamma-aminobutyric acid, the theanine of tea leaves, the xylo-oligosaccharide, the concentrated sour cherry powder, the passion flower powder, the spina date seed powder, the elderberry juice powder, the melissa powder and the edible essence is in the range, if the content of the German spring yellow chrysanthemum extract is lower than 0.01 part by weight, the effects of relieving pressure, relaxing mood and improving sleep are not obvious; if it is more than 1.0 part by weight, the taste of the beverage is not good; therefore, the content of the extract of German chamomilla is suitably 0.01 to 1.0 part by weight, preferably 0.02 to 1.0 part by weight, more preferably 0.03 to 0.8 part by weight;
when the content of the gamma-aminobutyric acid, the theanine, the xylo-oligosaccharide, the sour cherry concentrated powder, the passion flower powder, the spina date seed powder, the elderberry juice powder, the vespipe powder and the German chamomile extract is within the range, the content of the edible essence is not preferable if the content of the edible essence exceeds the range, and a consumer considers that the product is blended by the essence because the edible essence is too much.
Preferably, the composition of the drink composition is as follows: in parts by weight
Figure BDA0003551010140000131
The total amount of water is 100 parts by weight.
More preferably, the composition of the drink composition is as follows: in parts by weight
Figure BDA0003551010140000132
The total amount of water is 100 parts by weight.
According to the invention, the beverage composition contains 0.001-25.0 parts by weight of sweetening agent.
The sweetener is mainly used in the beverage composition for relieving pressure, relaxing mood and improving sleep of young people, and has the main effects of regulating the sweetness of the beverage and being easy to take.
The sweetener used in the present invention is one or more selected from the group consisting of white granulated sugar, erythritol, xylitol, isomalt, isomaltooligosaccharide, sucralose, acesulfame potassium, stevioside, and glycyrrhizin. They are all products currently on the market, such as white granulated sugar sold by Nanning sugar industry, Inc., erythritol sold by bowling Bao Biotechnology, Inc., xylitol sold by Zhejiang Huakang pharmaceutical industry, Inc., isomalt sold by Shandong Green Living Biotechnology, Inc., isomalto-oligosaccharide sold by bowling Bao Biotechnology, Inc., sucralose, stevioside, mogroside sold by Qianji Biotechnology, Inc., acesulfame sold by Qianji Biotechnology, Inc., glycyrrhizin sold by Shandong Kangqin Biotechnology, Inc.
In the present invention, any other sweetener which has the above-mentioned effects and does not adversely affect the performance and use of the beverage composition for stress relief, mood relaxation, and sleep improvement of young people of the present invention can be used in the present invention, and they are also within the scope of the present invention.
According to the invention, the content of the sweetener is 0.001 to 25.0 parts by weight. If the content of the sweetener is less than 0.001 weight part, the beverage composition product has no sweet taste; if the content of the sweetener is higher than 25.0 parts, the drink composition product is excessively sweet; therefore, the content of the sweetener is suitably 0.001 to 25.0 parts by weight;
according to the invention, the beverage composition contains 0.001-1 part by weight of an acid-base regulator.
The main effect of the acid-base regulator in the beverage composition for alleviating pressure, relaxing mood and improving sleep of young people is to regulate the acidity of the beverage, and the beverage is easy to take.
The pH adjustor used in the present invention is one or more acid-base adjusting agents selected from malic acid, citric acid, sodium citrate, lactic acid or fumaric acid, which are currently commercially available products, such as malic acid sold by Suzhou Minnie accurate nutrition science Co., Ltd, citric acid sold by Weifang Yingxuan industry Co., Ltd, sodium citrate, lactic acid sold by Henan Jindan lactic acid science Co., Ltd, and fumaric acid sold by Anhui Xuelan Bioscience Co., Ltd.
In the present invention, any other acid-base modifier which has the above-mentioned effects and does not adversely affect the performance and application of the beverage composition for young people to relieve stress, relax mood and improve sleep of the present invention can be used in the present invention, and they are also within the scope of the present invention.
According to the present invention, if the content of the acid-base modifier is less than 0.001 parts by weight, the sweetness and sourness of the product is poor; if the content of the acid-base regulator is higher than 1.0 part by weight, the sour taste of the product is too heavy; therefore, the content of the pH adjustor is preferably 0.001 to 1.0 part by weight.
The invention relates to a preparation method of a drink composition for alleviating pressure, relaxing mood and improving sleep of young people.
The preparation method comprises the following steps:
firstly, 0.08-4 parts by weight of sour cherry concentrated powder, 0.02-2.0 parts by weight of gamma-aminobutyric acid, 0.016-1.6 parts by weight of tea theanine, 0.02-2.0 parts by weight of wild jujube kernel powder, 0.12-12.0 parts by weight of xylo-oligosaccharide, 0.01-1.0 part by weight of passion fruit powder and 0.05-5.0 parts by weight of elderberry juice powder are uniformly stirred, then 0.001-1.0 part by weight of acid-base regulator and 0.001-25.0 parts by weight of sweetening agent are added, then the raw materials are added into 50 parts by weight of water and heated and dissolved at the temperature of 80 ℃, the obtained solution is filtered by using a plate-frame filter, and filtrate is collected;
secondly, adding 0.01 to 1.6 weight parts of balm grass powder, 0.01 to 1.0 weight part of German chamomile extract and 0.001 to 1.0 weight part of edible essence into the filtrate, uniformly stirring, and supplementing water until the total amount is 100 weight parts; and then cooling the temperature of the beverage to 45 ℃, filtering the beverage by using a plate-and-frame filter, collecting filtrate, filling the filtrate into a clean bottle, sealing the bottle, placing the bottle in a water bath sterilization cabinet, and sterilizing the bottle at the temperature of 105-115 ℃ for 20 minutes to obtain the beverage composition.
Or placing the collected filtrate in an ultrahigh-temperature instant sterilizer for sterilization, then placing the sterilized filtrate in a cleaning bottle, sealing the bottle, spraying the sterilized filtrate by a conveyor belt through a spray cooler, spraying the sterilized filtrate by hot water at 80-90 ℃ and cooling the sterilized filtrate by warm water at 25-40 ℃ to obtain the beverage composition.
The acid-base modifier used in the preparation method of the beverage composition is one or more acid-base modifiers selected from malic acid, citric acid, sodium citrate, lactic acid or fumaric acid; the sweetener used is one or more selected from white sugar, erythritol, xylitol, isomalt, isomaltooligosaccharide, sucralose, acesulfame potassium, stevioside, and glycyrrhizin. The basic aspects of these pH modifying agents and sweeteners have been described above and will not be described further herein.
According to the detection of the GB/T31326 standard method, the beverage composition for relieving pressure, relaxing mood and improving sleep of young people, which is prepared by the preparation method disclosed by the invention, has the following characteristics:
brown, slightly sweet, liquid with characteristic taste and smell. The nutritional ingredients of the drink composition are listed in table 4 below.
Table 4: the beverage composition of the invention contains nutrient components
Item Per 100mL NRV％
(Energy) 26-1000kJ 0-12％
Protein 0-5.0g 0-8％
Fat 0 0
Carbohydrate compound 1.5-50g 1-17％
Sodium salt 0-200g 0-10％
The drinking method of the beverage composition comprises the following steps: it is administered half an hour before sleep, and 25ml each time.
[ advantageous effects ]
According to the invention, through food with homology of medicine and food, gamma-aminobutyric acid is up-regulated and glutamic acid is down-regulated, so that the gamma-aminobutyric acid and the glutamic acid are balanced, the effects of relieving stress, anxiety and depression of young people, antagonizing excitation of caffeine and finally achieving the effect of improving sleep are achieved. The invention combines nine foods with food homology, has synergistic effect, and has the function of obviously improving sleep as proved by functional experiments of human bodies and zebra fishes.
[ description of the drawings ]
FIG. 1-1 is a zebra fish behavior trace diagram after 9-treatment after sleep
In the figure:
the black line represents the slow movement track of the zebra fish;
the light gray line represents medium speed motion;
dark gray lines represent fast motion;
FIG. 1-2 shows the wakefulness and activity of zebra fish treated with 9 days after sleep
P <0.05, p <0.001, compared to model control group
FIGS. 1-3 show the wake activity time of zebrafish after sleep 9 treatment
P <0.001 in comparison to model control group
FIG. 2-1 shows the relative expression level of gabra1 gene
P <0.01, p <0.001, compared to model control group
[ detailed description ] embodiments
The invention will be better understood from the following examples.
Example 1: the beverage composition of the invention
The implementation of this example is as follows.
The beverage composition comprises the following components: 0.3 weight part of gamma-aminobutyric acid, 0.5 weight part of theanine, 2.0 weight part of xylo-oligosaccharide, 0.8 weight part of concentrated sour cherry powder, 0.05 weight part of passion fruit powder, 1.0 weight part of elderberry juice powder, 0.5 weight part of wild jujube kernel powder, 0.05 weight part of wasp powder, 0.05 weight part of extract of German chamomile, 0.002 weight part of cranberry essence sold by Kaihong essence and spice of Guangzhou, 0.95 weight part of white granulated sugar sweetener sold by Nanning sugar industry Limited, 0.008 weight part of L-malic acid alkali regulator sold by accurate nutrition science and technology of Suzhou Mini, and water which is supplemented to 100 weight parts of the total amount.
The beverage composition is prepared by the following preparation method:
firstly, adding 0.3 weight part of gamma-aminobutyric acid, 0.5 weight part of tea theanine, 2.0 weight part of xylooligosaccharide, 0.8 weight part of cherry concentrated powder, 0.05 weight part of passion flower powder, 1.0 weight part of elderberry juice powder, 0.5 weight part of jujube kernel powder, 0.008 weight part of L-malic acid and 0.95 weight part of white granulated sugar into 50 weight parts of water, heating and dissolving at the temperature of 80 ℃, filtering the obtained solution by using a plate and frame filter, and collecting filtrate;
secondly, adding 0.05 weight part of balm bee powder, 0.05 weight part of German chamomile extract and 0.002 weight part of cranberry essence into the filtrate, uniformly stirring, and supplementing water until the total amount is 100 weight parts; and then, reducing the temperature to 45 ℃, filtering by using a plate-and-frame filter, filling the obtained filtrate into a clean bottle, sealing the bottle, and sterilizing at the temperature of 105-115 ℃ for 20 minutes to obtain the beverage composition.
Example 2: the beverage composition of the invention
The implementation of this example is as follows.
The beverage composition comprises the following components: 1.0 part by weight of gamma-aminobutyric acid, 0.8 part by weight of theanine, 2.5 parts by weight of xylo-oligosaccharide, 1.2 parts by weight of concentrated sour cherry powder, 0.08 part by weight of passion fruit powder, 0.8 part by weight of elderberry juice powder, 1.0 part by weight of wild jujube kernel powder, 0.05 part by weight of wasp powder, 0.06 part by weight of extract of german chamomile flower, 0.005 part by weight of rose essence sold by senxinxiang essence science and technology (china) limited, 5.2 parts by weight of white granulated sugar sweetener sold by nanning sugar industry limited, 0.012 part by weight of DL-malic acid alkali regulator sold by Suzhou Mini precision nutrition science and technology limited, and water which is supplemented to 100 parts by weight of the total amount.
The beverage composition is prepared by the following preparation method:
firstly, adding 1.0 weight part of gamma-aminobutyric acid, 0.8 weight part of theanine, 2.5 weight parts of xylo-oligosaccharide, 1.2 weight parts of concentrated sour cherry powder, 0.08 weight part of passion flower powder, 0.8 weight part of elderberry juice powder, 1.0 weight part of wild jujube kernel powder, 0.012 weight part of DL-malic acid and 5.2 weight parts of white granulated sugar into 50 weight parts of water, heating and dissolving at the temperature of 80 ℃, filtering the obtained solution by using a plate-and-frame filter, and collecting filtrate;
secondly, adding 0.05 weight part of balm bee powder, 0.06 weight part of German chamomile extract and 0.005 weight part of rose essence into the filtrate, uniformly stirring, and adding water until the total amount is 100 weight parts; then the temperature is reduced to 45 ℃, a plate-frame filter is used for filtering, the obtained filtrate is filled into a clean bottle, the cover is sealed, and the beverage composition is obtained after the sterilization is carried out for 20 minutes at the temperature of 105-.
Example 3: the beverage composition of the invention
The implementation of this example is as follows.
The beverage composition comprises the following components: 0.9 part by weight of gamma-aminobutyric acid, 1.2 parts by weight of theanine, 2.8 parts by weight of xylo-oligosaccharide, 2.5 parts by weight of sour cherry concentrate powder, 1.0 part by weight of passion fruit powder, 3.0 parts by weight of elderberry juice powder, 1.0 part by weight of wild jujube kernel powder, 0.5 part by weight of wasp powder, 0.3 part by weight of German yellow chrysanthemum extract, 0.015 part by weight of lemon essence sold by Hongtai Biotech Co., Ltd, 6.8 parts by weight of xylitol sweetener sold by Zhejiang Huakang pharmaceutical Co., Ltd, 0.015 part by weight of sodium citrate acid-base regulator sold by Weifang Yinxuan Xuan industry Co., Ltd, 0.015 part by weight of citric acid base regulator sold by Weifang Xuan industry Co., Ltd, and water in a total amount of 100 parts by weight.
The beverage composition is prepared by the following preparation method:
firstly, adding 0.9 weight part of gamma-aminobutyric acid, 1.2 weight parts of tea theanine, 2.8 weight parts of xylo-oligosaccharide, 2.5 weight parts of sour cherry concentrated powder, 1.0 weight part of passion fruit powder, 3.0 weight parts of elderberry juice powder, 1.0 weight part of wild jujube kernel powder, 0.015 weight part of sodium citrate, 0.015 weight part of citric acid and 6.8 weight parts of xylitol into 50 weight parts of water, heating and dissolving at the temperature of 80 ℃, filtering the obtained solution by using a plate and frame filter, and collecting filtrate;
secondly, adding 0.5 weight part of vespid bee powder, 0.3 weight part of German chamomile extract and 0.015 weight part of lemon essence into the filtrate, uniformly stirring, and adding water until the total amount is 100 weight parts; then the temperature is reduced to 45 ℃, a plate-frame filter is used for filtering, the obtained filtrate is filled into a clean bottle, the cover is sealed, and the beverage composition is obtained after the sterilization is carried out for 20 minutes at the temperature of 105-.
Example 4: the beverage composition of the invention
The implementation of this example is as follows.
The beverage composition comprises the following components: 1.5 parts by weight of gamma-aminobutyric acid, 1 part by weight of theanine, 2.8 parts by weight of xylo-oligosaccharide, 2.0 parts by weight of concentrated sour cherry powder, 0.4 part by weight of passion fruit powder, 2.4 parts by weight of elderberry juice powder, 1.4 parts by weight of spina date seed powder, 1.0 part by weight of cedar wasp powder, 1.0 part by weight of German chamomile extract, 0.025 part by weight of cranberry essence sold by Senxiang essence technology (China) Limited, 3.0 parts by weight of xylitol sweetener sold by Zhejiang Huakang pharmaceutical industry Limited, 0.024 part by weight of citrate acid alkali regulator sold by Weifang Yinxuan industry Limited, and water with the total amount being 100 parts by weight.
The beverage composition is prepared by the following preparation method:
firstly, 1.5 parts by weight of gamma-aminobutyric acid, 1.0 part by weight of tea theanine, 2.8 parts by weight of xylo-oligosaccharide, 2.0 parts by weight of sour cherry concentrated powder, 0.4 part by weight of passion fruit powder, 2.4 parts by weight of elderberry juice powder, 1.4 parts by weight of wild jujube kernel powder, 0.024 part by weight of citric acid and 3.0 parts by weight of xylitol are added into 50 parts by weight of water, the mixture is heated and dissolved at the temperature of 80 ℃, the obtained solution is filtered by a plate-and-frame filter, and the filtrate is collected;
secondly, adding 1.0 weight part of balm powder, 1.0 weight part of German chamomile extract and 0.025 weight part of cranberry essence into the filtrate, uniformly stirring, and adding water until the total weight is 100 weight parts; and then cooling the temperature to 45 ℃, filtering the filtrate by using a plate-and-frame filter, sterilizing the obtained filtrate by using an ultrahigh-temperature instantaneous sterilizer, filling the sterilized filtrate into a clean bottle, sealing the bottle, spraying the sterilized filtrate by a conveyor belt through a spray cooler, spraying the sterilized filtrate by hot water at the temperature of 80-90 ℃ and cooling the sterilized filtrate by warm water at the temperature of 25-40 ℃ to obtain the beverage composition.
Example 5: the beverage composition of the invention
The implementation of this example is as follows.
The beverage composition comprises the following components: 0.4 part by weight of gamma-aminobutyric acid, 0.08 part by weight of theanine, 4 parts by weight of xylo-oligosaccharide, 0.8 part by weight of sour cherry concentrated powder, 0.04 part by weight of passion fruit powder, 1.0 part by weight of elderberry juice powder, 0.4 part by weight of wild jujube kernel powder, 0.04 part by weight of wasp powder, 0.04 part by weight of German chamomile extract, 0.06 part by weight of elderberry essence sold by International (GmbH) Inc., 7.0 parts by weight of erythritol sweetener sold by bowling biont GmbH, 0.012 part by weight of sucralose sweetener sold by Mediterranean GmbH, 0.52 part by weight of DL-malic acid sold by Suzhou Miny Nutrition technology Inc., and water in a total amount of 100 parts by weight.
The beverage composition is prepared by the following preparation method:
firstly, 0.4 weight part of gamma-aminobutyric acid, 0.08 weight part of theanine, 4 weight parts of xylo-oligosaccharide, 0.8 weight part of cherry concentrate powder, 0.04 weight part of passion fruit powder, 1 weight part of elderberry juice powder, 0.4 weight part of jujube kernel powder, 0.52 weight part of DL-malic acid, 7.0 weight part of erythritol and 0.012 weight part of sucralose are added into 50 weight parts of water and heated and dissolved at the temperature of 80 ℃, the obtained solution is filtered by a plate-and-frame filter, and the filtrate is collected;
secondly, adding 0.04 weight part of balm powder, 0.04 weight part of German chamomile extract and 0.06 weight part of elderberry essence into the filtrate, stirring uniformly, and adding water until the total amount is 100 weight parts; and then cooling the temperature to 45 ℃, filtering the filtrate by using a plate-and-frame filter, sterilizing the obtained filtrate by using an ultrahigh-temperature instantaneous sterilizer, filling the sterilized filtrate into a clean bottle, sealing the bottle, spraying the sterilized filtrate by a conveyor belt through a spray cooler, spraying the sterilized filtrate by hot water at the temperature of 80-90 ℃ and cooling the sterilized filtrate by warm water at the temperature of 25-40 ℃ to obtain the beverage composition.
Test examples: clinical observation test of the drink composition of the invention
The procedure of this test example was as follows:
improving sleep animal observations
And (5) detecting an item I: improving sleep
1. Detection material
1.1. Test sample
The beverage composition stock solution of the invention prepared in example 5 was used directly, stored in the shade, sample No.: sleep well 9.
Positive control: diazepam, white tablets, lot No. 201001, shandongyi pharmaceutical limited, stored in the shade and in the dark. Stock solutions were prepared using DMSO (dimethyl sulfoxide) at 10.0mg/mL and stored at-20 ℃.
1.2. Laboratory animal
Reverse osmosis water is used, and 200mg of instant sea salt is added into every 1L of reverse osmosis water to prepare CaCO with the conductivity of 450-550 mu S/cm, the pH value of 6.5-8.5 and the hardness of 50-100 mg/L3The water for fish farming of (1).
Zebra fish, which was bred and supplied from a fish farming center of this company, was bred in this fish farming water at a temperature of 28 ℃. The license number for experimental animals is as follows: SYXK (Zhe) 2022-.
Wild type AB strain zebrafish, in a natural mated mating breeding mode. Zebrafish aged 5 days after fertilization (5dpf) were used for sample bedtime 9 Maximum Test Concentration (MTC) determination and sleep improvement efficacy evaluation.
1.3. Instruments, consumables and reagents
Dissecting microscope is SZX7, OLYMPUS, Japan, CCD camera is VertA1, shanghai tusson visual science and technology ltd, China; precision electronic balance CP214, OHAUS, USA; the 6-well plate was NestBiotech, China; the 96-well plate was NestBiotech, China; the behavioral analyzer was V3.11, ViewPointLife Sciences, France.
Pentylenetetrazole is PTZ, YH0171126, Shanghai Eichh Biotech, Inc., China; dimethyl sulfoxide was DMSO, lot No. BCCD8942, Sigma, Switzerland.
2. Detection method
MTC assay
And randomly selecting 5dpf wild type AB strain zebra fish in a 6-well plate, and treating 30 zebra fish in each well (experimental group). The sleep beverage composition aqueous solution of the invention is respectively administered to sleep 9 (the concentration is shown in table 1-1), and a normal control group and a model control group are simultaneously arranged, and the volume of each hole is 3 mL. After the treatment at the temperature of 28 ℃ for 24h, the other experimental groups except the normal control group are dissolved in water and are given PTZ to establish a zebra fish insomnia model, and after the treatment at the temperature of 28 ℃ for 1h, the MTC of the sleep-improving 9 zebra fish is determined.
2.2. Evaluation of efficacy in improving sleep
And randomly selecting 5dpf wild type AB strain zebra fish in a 6-well plate, and treating 30 zebra fish in each well (experimental group). The sleeping pills are dissolved in water respectively to give a sleeping pill of 9 (the concentration is shown in tables 1-2), the positive control diazepam is 10.0 mu g/mL, a normal control group and a model control group are simultaneously arranged, and the volume of each hole is 3 mL. After incubation in an incubator at 28 ℃ for 24h, the zebra fish is transferred to a 96-well plate, 200 mu L/tail and 1 tail/well, and except a normal control group, the rest experimental groups are all dissolved in water and are given to PTZ to establish a zebra fish insomnia model. And (3) detecting the awakening activity amount and the awakening activity time of the zebra fish by using a behavior analyzer, and evaluating the sleep improvement effect of 9 times of good sleep by using the statistical analysis result of the indexes. Statistical treatment results are expressed as mean ± SE. Statistical analysis was performed using SPSS26.0 software and p <0.05 indicated that the differences were statistically significant.
3. The result of the detection
3.1.MTC
Under the experimental condition, the maximum detection concentration (MTC) of 9 bedridden zebra fish with the sleep improving effect is 31.2 mu L/mL. See table 5 for details.
Table 5: good sleep 9 concentration groping test results for improving sleep (n ═ 30)
Figure BDA0003551010140000221
The results shown in Table 5 indicate that 9 sleeps well and the maximum detection concentration (MTC) of zebra fish with the sleep improving effect is 31.2 muL/mL.
3.2. Evaluation of efficacy in improving sleep
Under the experimental condition, the good sleep 9 has the effect of improving the sleep; in particular a reduction in the amount of and duration of wakefulness activity. See table 6, fig. 1-1, fig. 1-2, and fig. 1-3 for details.
Table 6: good sleep 9 evaluation of sleep efficacy (n ═ 10)
Figure BDA0003551010140000222
P <0.05, p <0.001, compared to model control group
The results of table 6, fig. 1-1, fig. 1-2, and fig. 1-3 clearly show that good sleep 9 has the effect of improving sleep; in particular a reduction in the amount of and duration of wakefulness activity.
And (5) detection item II: research on sleep improvement mechanism (Gene)
1. Detection material
1.1. Sample preparation
The detection item I is used for sleeping well 9, is directly used and is stored in the shade.
Positive control: diazepam, white tablets, lot No. 201001, shandongyi pharmaceutical limited, stored in the shade and in the dark. Diazepam was formulated as a stock solution at a concentration of 10.0mg/mL using DMSO and stored at-20 ℃.
1.2. Laboratory animal
Reverse osmosis water is used, and 200mg of instant sea salt is added into every 1L of reverse osmosis water to prepare CaCO with the conductivity of 450-550 mu S/cm, the pH value of 6.5-8.5 and the hardness of 50-100 mg/L3The water for fish farming of (1).
Zebra fish, which was bred and supplied from a fish farming center of this company, was bred in this fish farming water at a temperature of 28 ℃. The license number for experimental animals is as follows: SYXK (Zhe) 2022-.
Wild type AB strain zebrafish, in a natural mated mating breeding mode. Zebrafish of age 5dpf were used for evaluation of the effect of good sleep 9 on sleep-associated genes.
1.3. Instruments, consumables and reagents
Dissecting microscope is SZX7, OLYMPUS, Japan; the CCD camera is VertA1, Shanghai Tusen Vision science and technology Limited, China; precision electronic balance CP214, OHAUS, USA; the 6-well plate was Nest Biotech, China; common PCR amplificators are T100, BIO-RAD, Singapore; the fluorescent quantitative PCR instrument is CFX Connect, BIO-RAD, Singapore; the high speed refrigerated centrifuge is Heraeus Fresco17, ThermoFisher, Germany; the ultraviolet-visible spectrophotometer is Nanodrop2000, Thermo, USA; the microplate mini-centrifuge is BE-6100, China, Linbel instruments manufacture Inc. of Haimen; the lower skirt 96 well plate (clear) was D9161009B, Bio-rad, USA); the optically adhesive seal B was MSB1001, Bio-rad, USA.
PowerUp SYBR GreenMasterMix is Cat No. A25742, Sammer Feishell science and technology (China), Lithuania; the FastQuant RTkit (With gDNase) kit is TIANGEN, China; RNA-QuickPurificationkit (RNA rapid extraction kit is YIShanBiotech, China; pentylenetetrazol is PTZ, YH0171126, Eihh Biotech, Inc. of Shanghai, China; dimethylsulfoxide DMSO is Sigma, Switzerland).
2. Detection method
5dpf wild type AB strain zebra fish was randomly selected in a 6-well plate, and 30 zebra fish were treated per well (experimental group). 9 times of sleep (concentration shown in table 7 and tables 2-4) and 10.0 mug/mL of positive control diazepam are respectively dissolved in water, a normal control group and a model control group are simultaneously arranged, the volume of each hole is 3mL, and 3 biological repeated tests are parallelly arranged. After the treatment at the temperature of 28 ℃ for 24h, the other experimental groups except the normal control group are dissolved in water and are given PTZ to establish a zebra fish insomnia model. After treating for 1h at the temperature of 28 ℃, collecting samples of each group of zebra fish, extracting total RNA of each group of zebra fish by using an RNA rapid extraction kit, and determining the concentration and purity of the total RNA by using an ultraviolet-visible spectrophotometer. Mu.g of zebrafish sample total RNA (2.00 mu.g) is taken, 20.0 mu.L of cDNA is synthesized according to the operation of a cDNA first strand synthesis kit, and the expression of beta-actin, gabra1 and mtnr1aa (melatonin) genes is detected by q-PCR. The relative expression amounts of RNA of the genes gabra1 and mtnr1aa were calculated using β -actin as an internal reference for gene expression. Statistical treatment results are expressed as mean ± SE. Statistical analysis was performed using SPSS26.0 software and p <0.05 indicated that the differences were statistically significant.
3. The result of the detection
RNA extraction results and primer sequence information
After 9 times of sleep, extracting total RNA of the zebra fish, and determining the concentration of the RNA and the A260/A280 ratio (shown in table 7) by using an ultraviolet-visible spectrophotometer, wherein the A260/A280 ratio is 1.8-2.2, which shows that the extracted total RNA of the zebra fish has good quality and can be used for a subsequent q-PCR experiment. Primer sequences are shown in Table 8.
Table 7: total RNA concentration and a260/a280 ratio (n ═ 30)
Figure BDA0003551010140000241
Table 8: primer sequence information
Figure BDA0003551010140000251
3.2. Effect on sleep-related genes
Under the conditions of this experiment, sleep-good 9 was able to up-regulate the expression of gabra 1. See table 9, fig. 2-1 for details.
Table 9: effect of good sleep 9 on the expression of gabra1 Gene (n ═ 3)
Figure BDA0003551010140000252
P <0.05, p <0.01, p <0.001, compared to model controls
The results in Table 9, FIG. 2-1, clearly show that sleep 9 up-regulates the expression of gabra 1.
III, the beverage composition improves the sleep clinical observation
1.1 basic data of the experimenter
And selecting 90 company employees and family members meeting the inclusion standard, wherein the selected experimental objects are healthy and have no other diseases influencing experimental research.
1.1.1 inclusion criteria are as follows:
(i) age 18-44 years;
(ii) for the crowd with unsatisfactory sleep quality, screening the crowd by using a Pittsburgh sleep quality index questionnaire, and bringing qualified volunteers into a test;
(iii) the test is carried out voluntarily, and the questionnaire, the record and the biochemical detection can be completed;
(iv) the onset time is more than 1 month.
1.1.2 exclusion criteria
(i) Patients with serious diseases of cardiovascular and cerebrovascular diseases, liver, kidney and hemopoietic system, endocrine diseases, and psychosis;
(ii) the sleep improving medicine is not taken in a short time.
90 subjects who met the requirements were recruited in this trial.
1.2 drinking method:
the experimental group was drinking 25ml of the sample prepared in example 5 one hour before sleeping daily, sample number: 9, the sleep is good, the subjects take the medicine continuously for 4 weeks, and the curative effect evaluation is carried out after the taking of the medicine is stopped for 1 week, and the original dietary habits and normal diet are not changed during the drinking period.
1.3 observation indexes and judgment standards:
(i) comparing the daily sleep conditions before and after treatment, and adopting a Pittsburgh sleep quality index table (PSQI) to evaluate the daily sleep conditions, wherein the daily sleep conditions have 7 dimensions, each dimension is respectively counted into 0, 1, 2 and 3 according to four degrees of 'none-light-middle-heavy', the total score is 21, and the higher the score is, the worse the sleep quality is;
(ii) comparing the levels of amino acid neurotransmitters glutamic acid and gamma-aminobutyric acid before and after treatment, extracting 4ml of fasting venous blood from morning before and after treatment, and determining by adopting an enzyme-linked immunosorbent assay, wherein the higher the level of glutamic acid is, the more difficult the sleep is, and the higher the level of GABA is, the more easy the sleep is;
(iii) referring to the clinical research guiding principle of new Chinese medicine published by the Ministry of public health of the people's republic of China in 2002:
and (3) curing: the sleep time is more than 6h or the normal state is recovered, and the patient is full of consciousness and deep sleep after waking up.
The effect is shown: the sleep time is less than 6h, but can be increased to more than 3h, the sleep quality is obviously optimized, and the deep sleep time is obviously increased.
The method has the following advantages: the increase in sleep time was less than 3h, but the patient experienced improvement.
And (4) invalidation: the sleep time and quality are not obviously improved or even aggravated.
1.4 statistical methods
Statistical analysis is carried out on the obtained data by using SPSS 23.0 software, and the data is measured
Figure BDA0003551010140000261
The comparison between groups was performed by independent sample t test, and the comparison within groups was performed by paired t test. With P<0.05 difference was statistically significant.
1.5 therapeutic results
1.5.1 comparison of Pittsburgh scores before and after treatment.
The pittsburgh scores after treatment were all lower than before treatment, with statistical significance for the differences (P <0.05), see table 10.
Table 10: the PSQI scores before and after treatment were compared [ score,
Figure BDA0003551010140000271
]
group of Before treatment After treatment P value
Test set (n ═ 90) 10.45±1.05 3.15±1.04 <0.05
1.5.2 comparison of serum Glu and GABA levels before and after treatment.
Glu after treatment was lower than before treatment, GABA was higher than before treatment, and the differences were statistically significant (P <0.05), as shown in Table 11.
Table 11: comparing the levels of Glu and GABA in serum before and after treatment [ mg/L,
Figure BDA0003551010140000272
]
Figure BDA0003551010140000273
1.5.3 according to the guidance principle of clinical research on new Chinese medicines, which is formulated and released in 2002 by the people's republic of China, the effective rate results are shown in Table 12.
Table 12: effective rate table
Group of Cure of disease Cure rate Show effect Is effective Invalidation Total effective rate
Test set (n ═ 90) 60 66.7％ 20 5 5 94.4％
1.6 adverse reactions
The drink composition for relieving pressure, relaxing mood and improving sleep of young people does not have any adverse reaction in the clinical treatment and observation process.
1.7 conclusion
The clinical statistical results show that the beverage composition has the advantages of obvious clinical curative effect, eating safety and good taste in the aspect of treating the insomnia of young people, and is worthy of popularization and application in clinical practice of young insomnia people.

Claims (10)

1. A beverage composition for alleviating stress, relaxing mood and improving sleep in young people, which is characterized by comprising the following components: in parts by weight
Figure FDA0003551010130000011
2. The beverage composition according to claim 1, wherein the composition of the beverage composition is as follows: in parts by weight
Figure FDA0003551010130000012
3. The beverage composition according to claim 1, wherein the composition of the beverage composition is as follows: in parts by weight
Figure FDA0003551010130000013
4. The beverage composition according to any one of claims 1 to 3, wherein the composition comprises 0.001 to 25.0 parts by weight of a sweetener.
5. A composition according to claim 4, characterised in that the sweetener is one or more sweeteners selected from the group consisting of white granulated sugar, erythritol, xylitol, isomalt, isomaltooligosaccharides, sucralose, acesulfame potassium, stevia or glycyrrhizin.
6. The beverage composition according to any one of claims 1 to 3, wherein the beverage composition comprises 0.001 to 1 part by weight of the pH modifier.
7. The beverage composition according to claim 6, wherein the pH modifier is one or more pH modifiers selected from malic acid, citric acid, sodium citrate, lactic acid and fumaric acid.
8. A preparation method of a beverage composition for alleviating stress, relaxing mood and improving sleep of young people is characterized by comprising the following steps:
firstly, 0.08-4 parts by weight of concentrated sour cherry powder, 0.02-2.0 parts by weight of gamma-aminobutyric acid, 0.016-1.6 parts by weight of tea theanine, 0.02-2.0 parts by weight of spine date seed powder, 0.12-12.0 parts by weight of xylo-oligosaccharide, 0.01-1.0 part by weight of passion flower powder and 0.05-5.0 parts by weight of elderberry juice powder are uniformly stirred, then 0.001-1.0 part by weight of acid-base regulator and 0.001-25.0 parts by weight of sweetening agent are added into 50 parts by weight of water and are heated and dissolved at the temperature of 80 ℃, the obtained solution is filtered by a plate-and-frame filter, and filtrate is collected;
secondly, adding 0.01 to 1.6 weight parts of balm grass powder, 0.01 to 1.0 weight part of German chamomile extract and 0.001 to 1.0 weight part of edible essence into the filtrate, uniformly stirring, and supplementing water until the total amount is 100 weight parts; and then, reducing the temperature of the drink to 45 ℃, filtering the drink by using a plate-and-frame filter, collecting filtrate, filling the filtrate into a clean bottle, sealing a cover, placing the bottle in a water bath sterilization cabinet, and sterilizing the bottle for 20 minutes at the temperature of 105-115 ℃ to obtain the drink composition.
9. The preparation method according to claim 8, wherein the collected filtrate is sterilized in an ultra-high temperature instant sterilizer, the sterilized filtrate is filled into a clean bottle, the bottle is sealed, and the beverage composition is obtained by passing through a conveyor belt, a spray cooler, hot water spraying at 80-90 ℃ and warm water cooling at 25-40 ℃.
10. The method according to claim 8, wherein the pH regulator is one or more pH regulators selected from malic acid, citric acid, sodium citrate, lactic acid and fumaric acid; the sweetener is one or more selected from white sugar, erythritol, xylitol, isomalt, isomaltose hypgather, sucralose, acesulfame potassium, stevioside, and glycyrrhizin.
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