CN114634855A - Microbial beneficial bacterium culture apparatus - Google Patents
Microbial beneficial bacterium culture apparatus Download PDFInfo
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- CN114634855A CN114634855A CN202210232426.4A CN202210232426A CN114634855A CN 114634855 A CN114634855 A CN 114634855A CN 202210232426 A CN202210232426 A CN 202210232426A CN 114634855 A CN114634855 A CN 114634855A
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- 230000009286 beneficial effect Effects 0.000 title claims abstract description 21
- 241000894006 Bacteria Species 0.000 title claims abstract description 17
- 230000000813 microbial effect Effects 0.000 title claims description 3
- 238000003756 stirring Methods 0.000 claims abstract description 60
- 244000005700 microbiome Species 0.000 claims abstract description 57
- 239000001963 growth medium Substances 0.000 claims abstract description 55
- 230000001681 protective effect Effects 0.000 claims description 26
- 238000003825 pressing Methods 0.000 claims description 13
- 238000005096 rolling process Methods 0.000 claims description 13
- 238000012258 culturing Methods 0.000 claims description 12
- 239000011521 glass Substances 0.000 claims description 10
- 235000015097 nutrients Nutrition 0.000 abstract description 22
- 239000002609 medium Substances 0.000 abstract description 18
- 230000000694 effects Effects 0.000 abstract description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000186361 Actinobacteria <class> Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/46—Means for fastening
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to the field of microorganism culture devices, in particular to a microorganism beneficial bacterium culture device which comprises a device body, a stirring structure, a culture medium, a supporting structure and an adjusting structure, wherein the stirring structure is arranged on the device body; the device body is internally provided with a stirring structure, a supporting structure and an adjusting structure; the stirring structure is fixedly connected with the inner wall of the upper part of the device body; the supporting structure is positioned in the middle of the device body; the puddler that sets up can mix microorganism and nutrient medium in the culture medium according to certain law under the drive of first motor, the puddler of vertical setting can not stir microorganism and nutrient medium to the side inner wall of culture medium when stirring microorganism and nutrient medium, the external gear and the interior gear of while through setting up mutually support, adjust the rotation axle center of puddler, the puddler can not rotate around only axle center, but improve the mixed effect of stirring structure to microorganism and nutrient medium.
Description
Technical Field
The invention relates to the field of microorganism culture devices, in particular to a microorganism beneficial bacterium culture device.
Background
The microbial culture is one of biological culture, the cultured microorganisms mainly comprise viruses, bacteria, actinomycetes, fungi and the like, a culture device is required to assist in the process of culturing the microorganisms so as to provide a proper environment for the survival and the propagation of the microorganisms, a culture medium is a mixture of nutrient substances which are manually prepared according to a certain proportion and are required for the growth and the propagation of the microorganisms and the synthesis of metabolites, the raw materials of the culture medium can be divided into a carbon source, a nitrogen source, inorganic salts, growth factors and water, and after the microorganisms to be inoculated are inoculated into the culture medium, certain shaking or stirring is required to be carried out, so that the microorganisms are uniformly distributed in the nutrient substances arranged in the culture medium.
However, the existing culture device is used, when the inoculated microorganisms are stirred, the microorganisms are easily adhered to the side wall of the culture medium and cannot be uniformly mixed with the nutrient medium; inconvenient whole the taking out of culture medium that will accomplish the cultivation when carrying out batch culture, need wait for the first batch accomplish observe and can cultivate new microorganism again after studying, influence researcher's work efficiency.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a device for culturing beneficial bacteria of microorganisms.
The technical scheme adopted by the invention for solving the technical problem is as follows: a microorganism beneficial bacterium culture device comprises a device body, a stirring structure, a culture medium, a supporting structure and an adjusting structure; the device body is internally provided with a stirring structure, a supporting structure and an adjusting structure; the stirring structure is fixedly connected with the inner wall of the upper part of the device body; the supporting structure is positioned in the middle of the device body, and a culture medium is placed on the supporting structure; the adjusting structure is positioned below the supporting structure; the device body comprises a device shell, and a supporting seat is fixedly connected to the inner wall of the device shell.
Specifically, the stirring structure comprises an electric push rod, a first motor, a fixed frame, a stirring rod, an external gear, an internal gear, a mounting plate, a limiting spring, a connecting rod and a mounting frame; the upper end of the electric push rod is fixedly connected with the inner wall of the upper part of the device shell, the lower end of the electric push rod is fixedly connected with a fixed frame through a mounting frame, the upper surface of the fixed frame is fixedly connected with a first motor, an output shaft of the first motor penetrates through the fixed frame and is fixedly connected with a mounting plate, a connecting rod is connected onto the mounting plate in a sliding mode, one end, located outside the mounting plate, of the connecting rod is fixedly connected with an outer gear, and a limiting spring is arranged inside the mounting plate; the inner wall of mount is provided with the internal gear, internal gear and external gear intermeshing, the lower surface fixedly connected with puddler of external gear.
Specifically, the supporting structure comprises a supporting frame, a first connecting shaft, a fixed seat, a clamping arm, a first spring, a clamping block and a supporting plate; the supporting frame is placed on the supporting seat, the lower surface of the supporting frame is fixedly connected with a first connecting shaft, the upper part of the supporting frame is fixedly connected with a fixed seat, the fixed seats are distributed on the supporting frame in an annular array mode, clamping arms are rotatably connected to the fixed seat, the clamping arms are distributed on the fixed seat in an annular array mode, and the upper ends of the clamping arms are fixedly connected with clamping blocks; the inside fixedly connected with first spring of fixing base, the upper end fixedly connected with supporting disk of first spring, the lower surface and the centre gripping arm lower end upper surface contact of supporting disk.
Specifically, the adjusting structure comprises a pressure lever, a second motor, a second connecting shaft, a second spring, a fixed disc, a bearing, a first fixed rod, a large roller, a second fixed rod and a small roller; the second motor is fixedly connected with the inner wall of the lower part of the device shell, an output shaft of the second motor is connected with a second connecting shaft in a sliding manner, and the upper end of the second connecting shaft is matched with the lower end of the first connecting shaft; a second spring is sleeved on an output shaft of the second motor, and the inner diameter of the second spring is larger than the outer diameter of the second connecting shaft; the lower part of the second connecting shaft is rotatably connected with a fixed disc through a bearing, first fixed rods are symmetrically arranged on the fixed disc, the first fixed rods are fixedly connected with the fixed disc, and one ends of the first fixed rods, which are far away from the second connecting shaft, are rotatably connected with large idler wheels; the pressing rod is fixedly connected to the second connecting shaft, a second fixing rod is fixedly connected to the middle of the pressing rod, the second fixing rods are symmetrically arranged about the central axis of the pressing rod, and the end, far away from the pressing rod, of the second fixing rod is rotatably connected with a small roller.
Specifically, the device body comprises a device shell, a limiting groove, a protective door, glass, a handle, a supporting seat, a baffle, a rolling shaft and a ball; the supporting seat is positioned in the middle of the device shell, the supporting seat is rotatably connected with rolling shafts, the rolling shafts are arranged on the supporting seat at equal intervals and are in contact with the edge position of the supporting frame, the positions, below the supporting frame, of the supporting seat are provided with rolling balls, the rolling balls are distributed on the supporting seat at equal intervals in an annular array mode, and the rolling balls are in rolling connection with the supporting seat; the device comprises a device shell, a protective door, a glass handle and a handle, wherein the protective door is rotatably connected to the device shell, the protective door is symmetrically arranged on a vertical plane where a symmetry axis of the device shell is located, the protective door is provided with the glass and the handle, and the handle is fixedly connected with the corresponding protective door; the position department that the device shell inner wall is located big gyro wheel both sides all is provided with the baffle, just the position department that the inner wall department of device shell corresponds little gyro wheel all has seted up the spacing groove.
The invention has the beneficial effects that:
(1) according to the beneficial microorganism culture device, the stirring structure is arranged, after a culture medium is placed on the supporting structure, the stirring rod arranged in the stirring structure can be driven by the first motor to mix the microorganisms and the nutrient medium in the culture medium according to a certain rule, the vertically arranged stirring rod cannot stir the microorganisms and the nutrient medium to the inner wall of the side face of the culture medium when the microorganisms and the nutrient medium are stirred, meanwhile, the rotating axis of the stirring rod is adjusted through the mutual matching of the arranged outer gear and the arranged inner gear, the stirring rod cannot rotate around the unique axis, and the mixing effect of the stirring structure on the microorganisms and the nutrient medium is improved.
(2) According to the beneficial microorganism culture device, the supporting structure and the adjusting structure are matched for use, after the culture of microorganisms is completed, the connection between the first connecting shaft and the second connecting shaft is cancelled through the pressure rod in the adjusting structure, so that the supporting structure and a culture medium placed on the supporting structure can be conveniently taken down from the device body, the supporting structure is replaced for culture of microorganisms of the next batch, the culture is performed without waiting for the completion of observation and research of the first batch, the efficiency of workers is improved, meanwhile, the adjusting structure can be used for driving the supporting structure to rotate, the positions of different angles are adjusted, and the stirring structure can be used for stirring different culture mediums on the supporting structure conveniently.
Drawings
The invention is further illustrated by the following examples in conjunction with the drawings.
FIG. 1 is a schematic structural view of a microorganism beneficial bacterium culture device provided by the invention;
FIG. 2 is a schematic view of the internal structure of the present invention;
FIG. 3 is a side cross-sectional view of the present invention;
FIG. 4 is a schematic view showing the internal structure of the medium of FIG. 1 according to the present invention;
FIG. 5 is a front cross-sectional view of the present invention;
FIG. 6 is an enlarged schematic view of the support structure of FIG. 1 according to the present invention;
FIG. 7 is an enlarged view of the adjustment structure of FIG. 1 according to the present invention;
FIG. 8 is an enlarged view of the support base shown in FIG. 1;
FIG. 9 is a cross-sectional view of the stirring structure of FIG. 1 in accordance with the present invention;
FIG. 10 is an enlarged view of point A of FIG. 5 according to the present invention.
In the figure: 1. a device body; 11. a device housing; 11a, a limiting groove; 12. a protective door; 12a, glass; 12b, a handle; 13. a supporting seat; 14. a baffle plate; 15. a roller; 16. a ball bearing; 2. a stirring structure; 21. an electric push rod; 22. a first motor; 23. a fixed mount; 24. a stirring rod; 25. an outer gear; 26. an internal gear; 27. mounting a plate; 27a, a limit spring; 28. a connecting rod; 29. a mounting frame; 3. a culture medium; 4. a support structure; 41. a support frame; 42. a first connecting shaft; 43. a fixed seat; 44. a clamp arm; 45. a first spring; 46. a clamping block; 47. a support disc; 5. adjusting the structure; 51. a pressure lever; 52. a second motor; 53. a second connecting shaft; 54. a second spring; 55. fixing the disc; 56. a bearing; 57. a first fixing lever; 58. a large roller; 59. a second fixing bar; 59a, small rollers.
Detailed Description
The present invention will be further described with reference to the following detailed description so that the technical means, the creation features, the achievement purposes and the effects of the present invention can be easily understood.
As shown in fig. 1-10, the beneficial microbe culture device according to the present invention comprises a device body 1, a stirring structure 2, a culture medium 3, a support structure 4 and an adjusting structure 5; the stirring device comprises a device body 1, wherein a stirring structure 2, a supporting structure 4 and an adjusting structure 5 are arranged in the device body 1; the stirring structure 2 is fixedly connected with the inner wall of the upper part of the device body 1; the supporting structure 4 is positioned in the middle of the device body 1, and the culture medium 3 is placed on the supporting structure 4; the adjusting structure 5 is located below the supporting structure 4; the device body 1 comprises a device shell 11, and a supporting seat 13 is fixedly connected to the inner wall of the device shell 11; the device body 1 is used for providing a closed environment for culturing microorganisms, a temperature control structure and a humidity control structure are arranged inside the device body 1, a proper culture environment can be provided during microorganism culture, the stirring structure 2 is arranged, after inoculation is completed and the culture medium 3 is placed on the supporting structure 4, the microorganism inoculated in the culture medium 3 can be uniformly mixed with the nutrient medium by the stirring structure 2, the inner side wall of the culture medium 3 can be ensured not to be adhered to the microorganisms and the nutrient medium, and the culture medium 3 can be cleaned in the later period and the cultured microorganisms can be conveniently observed by workers; the supporting structure 4 can support the culture medium 3, and meanwhile, the position of the culture medium 3 can be conveniently adjusted, so that the stirring structure 2 can be used for stirring different culture media 3, the adjusting structure 5 can drive the supporting structure 4 to integrally rotate, the position of the culture medium 3 is adjusted, different culture media 3 are moved to the position under the stirring structure 2, the stirring structure 2 can conveniently stir microorganisms and nutrient media in the culture medium 3 on the supporting structure 4, and meanwhile, the adjusting structure 5 can cancel the limitation on the supporting structure 4, so that the supporting structure 4 can be conveniently taken out integrally; the device shell 11 is a main structure of the device body 1, so that the stirring structure 2, the supporting structure 4 and the adjusting structure 5 are convenient to fix, and the supporting seat 13 arranged in the device shell 11 can stably support the supporting structure 4.
Specifically, the stirring structure 2 comprises an electric push rod 21, a first motor 22, a fixed bracket 23, a stirring rod 24, an external gear 25, an internal gear 26, a mounting plate 27, a limiting spring 27a, a connecting rod 28 and a mounting bracket 29; the upper end of the electric push rod 21 is fixedly connected with the inner wall of the upper part of the device shell 11, the lower end of the electric push rod 21 is fixedly connected with a fixed frame 23 through a mounting frame 29, the upper surface of the fixed frame 23 is fixedly connected with a first motor 22, an output shaft of the first motor 22 penetrates through the fixed frame 23 and is fixedly connected with a mounting plate 27, the mounting plate 27 is connected with a connecting rod 28 in a sliding manner, one end, positioned outside the mounting plate 27, of the connecting rod 28 is fixedly connected with an outer gear 25, and a limiting spring 27a is arranged inside the mounting plate 27; the inner wall of the fixed frame 23 is provided with an inner gear 26, the inner gear 26 is meshed with an outer gear 25, and the lower surface of the outer gear 25 is fixedly connected with a stirring rod 24; the electric push rod 21 is used for adjusting the height of the stirring structure 2, the stirring rod 24 in the stirring structure 2 cannot influence the rotation of the supporting structure 4, and the mounting frame 29 is arranged to reserve a certain space for the arrangement of the first motor 22 while the mounting frame 23 is conveniently connected with the electric push rod 21; the fixed frame 23 can fix the inner gear 26, the outer gear 25 and the inner gear 26 are meshed, so that the outer gear 25 can revolve around the inner gear 26 while rotating under the driving of the first motor 22, the running track of the stirring rod 24 is adjusted, the stirring rod 24 can mix nutrient media and microorganisms in the culture medium 3 in a vertical state, the microorganisms or the nutrient media cannot stick to the inner wall of the culture medium 3, workers can conveniently observe the cultured microorganisms while cleaning the culture medium 3, the connecting rod 28 can be driven by the outer gear 25 to slide on the mounting plate 27 when rotating, and the outer gear 25 can be meshed with the inner gear 26 all the time to revolve; the stopper spring 27a provided inside the mounting plate 27 applies an urging force to the connecting rod 28 so as to ensure that the external gear 25 does not disengage from the internal gear 26 during rotation and revolution, and the stopper spring 27a is provided at one end of the mounting plate 27 close to the output shaft of the first motor 22.
Specifically, the supporting structure 4 includes a supporting frame 41, a first connecting shaft 42, a fixing seat 43, a clamping arm 44, a first spring 45, a clamping block 46 and a supporting plate 47; the supporting frame 41 is placed on the supporting base 13, the lower surface of the supporting frame 41 is fixedly connected with the first connecting shaft 42, the upper part of the supporting frame 41 is fixedly connected with a fixed base 43, the fixed bases 43 are distributed on the supporting frame 41 in an annular array, the fixed base 43 is rotatably connected with clamping arms 44, the clamping arms 44 are distributed on the fixed base 43 in an annular array, and the upper ends of the clamping arms 44 are fixedly connected with clamping blocks 46; a first spring 45 is fixedly connected inside the fixed seat 43, the upper end of the first spring 45 is fixedly connected with a supporting plate 47, and the lower surface of the supporting plate 47 is in contact with the upper surface of the lower end of the clamping arm 44; the whole structure of the supporting structure 4 is circular, the central axis of the first connecting shaft 42 coincides with the central axis of the supporting frame 41, the fixing seat 43 facilitates the arrangement of the first spring 45 and the rotating connection of the clamping arm 44, when the culture medium 3 is placed on the supporting disc 47, the acting force of the supporting disc 47 on the first spring 45 is increased, the first spring 45 is contracted, meanwhile, the support plate 47 moves downwards and pushes the clamping arm 44 to rotate, the upper end of the clamping arm 44 gathers towards the culture medium 3, the culture medium 3 is fixed, the culture medium 3 can be in a fixed state when rotating along with the support structure 4, when the stirring structure 2 mixes the microorganisms and the nutrient medium in the culture medium 3, the culture medium 3 cannot rotate or displace, and the clamping block 46 is made of rubber, so that the surface of the culture medium 3 cannot be damaged while the friction force between the clamping arm 44 and the culture medium 3 is increased.
Specifically, the adjusting structure 5 includes a pressing rod 51, a second motor 52, a second connecting shaft 53, a second spring 54, a fixed disk 55, a bearing 56, a first fixing rod 57, a large roller 58, a second fixing rod 59 and a small roller 59 a; the second motor 52 is fixedly connected with the inner wall of the lower part of the device shell 11, the output shaft of the second motor 52 is connected with a second connecting shaft 53 in a sliding way, and the upper end of the second connecting shaft 53 is matched with the lower end of the first connecting shaft 42; a second spring 54 is sleeved on an output shaft of the second motor 52, and the inner diameter of the second spring 54 is larger than the outer diameter of the second connecting shaft 53; the lower part of the second connecting shaft 53 is rotatably connected with a fixed disc 55 through a bearing 56, the fixed disc 55 is symmetrically provided with first fixed bars 57, the first fixed bars 57 are fixedly connected with the fixed disc 55, and one ends of the first fixed bars 57 far away from the second connecting shaft 53 are rotatably connected with large rollers 58; a pressing rod 51 is fixedly connected to the second connecting shaft 53, a second fixing rod 59 is fixedly connected to the middle of the pressing rod 51, the second fixing rods 59 are symmetrically arranged about the central axis of the pressing rod 51, and one end of each second fixing rod 59, which is far away from the pressing rod 51, is rotatably connected with a small roller 59 a; when the press rod 51 is pressed downwards, the press rod 51 can drive the fixed disc 55 to move downwards integrally, at the moment, the second fixed rod 59 arranged on the press rod 51 can slide in the limiting groove 11a formed in the device shell 11 through the small roller 59a, so that the press rod 51 can be ensured to vertically move downwards, the first fixed rod 57 arranged on the fixed disc 55 can slide on the inner wall of the device shell 11 through the large roller 58 to limit the fixed disc 55, the fixed disc 55 can only move in the vertical direction, the fixed disc 55 can limit the upper end of the second spring 54 and simultaneously drive the second connecting shaft 53 to move upwards and downwards, so that the second connecting shaft 53 is separated from the first connecting shaft 42, the limit of the second connecting shaft 53 on the first connecting shaft 42 is cancelled, the support structure 4 can be conveniently taken down integrally by a worker, and the working efficiency of the worker is improved; the arrangement of the bearing 56 is convenient for fixing the fixed disk 55, and does not influence the output shaft of the second motor 52 to drive the second connecting shaft 53 to rotate; a clamping block is arranged on the output shaft of the second motor 52, and a sliding groove is formed in a position of the clamping block corresponding to the second connecting shaft 53, and the sliding groove can limit the position of the clamping block, so that when the second motor 52 rotates, the second connecting shaft 53 can rotate together.
Specifically, the device body 1 comprises a device shell 11, a limiting groove 11a, a protective door 12, glass 12a, a handle 12b, a supporting seat 13, a baffle plate 14, a rolling shaft 15 and a rolling ball 16; the supporting seat 13 is positioned in the middle of the device shell 11, the supporting seat 13 is rotatably connected with rollers 15, the rollers 15 are arranged on the supporting seat 13 at equal intervals, the rollers 15 are all contacted with the edge position of the supporting frame 41, balls 16 are arranged on the supporting seat 13 at the position below the supporting frame 41, the balls 16 are distributed on the supporting seat 13 at equal intervals in an annular array, and the balls 16 are in rolling connection with the supporting seat 13; the device comprises a device shell 11, a protective door 12, a glass 12a and a handle 12b, wherein the protective door 12 is connected to the device shell 11 in a rotating mode, the protective door 12 is symmetrically arranged on a vertical plane where a symmetry axis of the device shell 11 is located, the protective door 12 is provided with the glass 12a and the handle 12b, and the handle 12b is fixedly connected with the corresponding protective door 12; baffles 14 are arranged at the positions, located on the two sides of the large idler wheel 58, of the inner wall of the device shell 11, and limiting grooves 11a are formed in the positions, corresponding to the small idler wheels 59a, of the inner wall of the device shell 11; the limiting groove 11a is arranged to enable the small roller 59a to roll up and down only in the vertical direction, the angle of the pressure lever 51 on the horizontal plane is limited through the small roller 59a and the second fixing rod 59, when the pressure lever 51 moves downwards, horizontal acting force cannot be applied to the fixed disc 55, the baffle plate 14 is arranged to limit the rolling track of the large roller 58, the angle of the fixed disc 55 in the horizontal direction is limited through the matching between the large roller 58 and the first fixing rod 57, the fixed disc 55 is used in cooperation with the second fixing rod 59 and the small roller 59a, the fixed disc 55 can drive the second connecting shaft 53 to move up and down more stably, meanwhile, the fixed disc 55 is limited not to rotate, the using effect of the second spring 54 can be improved, the second spring 54 is prevented from being acted by the horizontal acting force, and the second spring 54 is protected; the protective door 12 is convenient for workers to place the culture medium 3 on the supporting structure 4 or take the supporting structure 4 out of the device shell 11 integrally, the handle 12b arranged on the protective door 12 is convenient for the workers to open or close the protective door 12, and the glass 12a arranged on the protective door 12 can observe the growth condition of microorganisms in the culture medium 3 without opening the protective door 12 when the workers culture; roller bearing 15 and the ball 16 that set up on supporting seat 13 can reduce the frictional force between supporting seat 13 and the bearing structure 4, make things convenient for bearing structure 4 to rotate, and the setting of ball 16 makes things convenient for the whole outside pulling of bearing structure 4 after the staff cancels the restriction to bearing structure 4 through adjustment structure 5 simultaneously, and up to bearing structure 4 can follow supporting seat 13 and take off.
Firstly, the protective door 12 is opened through the handle 12b, then nutrient medium is placed in the culture medium 3, then microorganisms are inoculated into the nutrient medium arranged in the culture medium 3, the culture medium 3 is placed downwards from the upper part of the supporting plate 47 in the supporting structure 4, after the lower surface of the culture medium 3 is contacted with the supporting plate 47, the supporting plate 47 pushes the first spring 45 downwards to deform, meanwhile, the supporting plate 47 pushes the clamping arm 44 to rotate, the upper end of the clamping arm 44 is close to the culture medium 3, when the deformation of the first spring 45 is maximum, the upper end of the clamping arm 44 drives the clamping block 46 to fix the culture medium 3 on the supporting structure 4, after the placement of the culture medium 3 is completed, the electric push rod 21 is started, the electric push rod 21 pushes the stirring structure 2 downwards, when the electric push rod 21 is extended to the maximum length, the lower end of the stirring rod 24 enters the culture medium 3 and is close to the inner wall of the bottom of the culture medium 3, starting the first motor 22, the first motor 22 drives the mounting plate 27 to rotate, the mounting plate 27 drives the external gear 25 to rotate through the connecting rod 28 while rotating, the external gear 25 is attached to the internal gear 26 to revolve, along with the revolution of the external gear 25, the upper end of the connecting rod 28 slides in the mounting plate 27 and extrudes the limiting spring 27a to deform, so as to ensure that the external gear 25 is always meshed with the internal gear 26 during revolution, the stirring rod 24 is driven during the rotation of the external gear 25 to stir the microorganisms and the nutrient medium in the culture medium 3, so that the microorganisms and the nutrient medium are fully mixed, the second motor 52 is started, the first connecting shaft 42 is driven to rotate by the second motor 52 through the second connecting shaft 53, the first connecting shaft 42 drives the supporting structure 4 to integrally rotate, the positions of different fixing seats 43 are adjusted, after the microorganisms and the nutrient medium in the culture medium 3 on different fixing seats 43 are mixed by the stirring structure 2, the electric push rod 21 contracts to drive the stirring structure 2 to recover to the original height, then the protective door 12 is closed, and the temperature and the humidity in the device body 1 are set, so that a proper environment is provided for the culture of microorganisms; after the microorganisms are cultured, the protective door 12 is opened through the handle 12b, then the pressure rod 51 is pressed downwards, the pressure rod 51 drives the fixed disk 55 to move downwards together, the fixed disk 55 drives the second connecting shaft 53 to move downwards through the bearing 56, when the second connecting shaft 53 is completely separated from the first connecting shaft 42, the supporting structure 4 is pulled outwards, the supporting structure 4 slides outwards on the supporting seat 13, the ball 16 rolls under the friction of the supporting frame 41, the friction resistance on the outward sliding of the supporting structure 4 is reduced, after the supporting structure 4 is completely taken down, the microorganisms in the culture medium 3 can be observed and researched, after the temperature and the humidity in the device body 1 are adjusted, the inoculation of the microorganisms of the next batch can be started, meanwhile, different supporting structures 4 can be replaced to stir and culture the configured microorganisms again, and the efficiency of the microorganism culture is accelerated, the convenience of workers is studied.
The foregoing shows and describes the general principles, principal features and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and the embodiments and descriptions given above are only illustrative of the principles of the present invention, and various changes and modifications may be made without departing from the spirit and scope of the invention, which fall within the scope of the claims. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (10)
1. A microbial beneficial bacteria culture device is characterized in that: comprises a device body (1), a stirring structure (2), a culture medium (3), a supporting structure (4) and an adjusting structure (5); the device comprises a device body (1), wherein a stirring structure (2), a supporting structure (4) and an adjusting structure (5) are arranged in the device body; the stirring structure (2) is fixedly connected with the inner wall of the upper part of the device body (1); the supporting structure (4) is positioned in the middle of the device body (1), and a culture medium (3) is placed on the supporting structure (4); the adjusting structure (5) is positioned below the supporting structure (4); the device body (1) comprises a device shell (11), and a supporting seat (13) is fixedly connected to the inner wall of the device shell (11).
2. The apparatus for culturing beneficial bacteria of microorganisms according to claim 1, wherein: the stirring structure (2) comprises an electric push rod (21), a first motor (22), a fixed frame (23), a stirring rod (24), an external gear (25), an internal gear (26), a mounting plate (27), a limiting spring (27a), a connecting rod (28) and a mounting frame (29); the upper end of electric putter (21) and the upper portion inner wall fixed connection of device shell (11), just the lower extreme of electric putter (21) passes through mounting bracket (29) and mount (23) fixed connection, the last fixed surface of mount (23) is connected with first motor (22).
3. The apparatus for culturing beneficial bacteria of microorganisms according to claim 2, wherein: an output shaft of the first motor (22) penetrates through a fixed frame (23) and is fixedly connected with a mounting plate (27), the mounting plate (27) is connected with a connecting rod (28) in a sliding mode, one end, located outside the mounting plate (27), of the connecting rod (28) is fixedly connected with an outer gear (25), and a limiting spring (27a) is arranged inside the mounting plate (27); the inner wall of the fixed frame (23) is provided with an internal gear (26), the internal gear (26) is meshed with an external gear (25), and the lower surface of the external gear (25) is fixedly connected with a stirring rod (24).
4. The apparatus for culturing beneficial bacteria of microorganisms according to claim 3, wherein: the supporting structure (4) comprises a supporting frame (41), a first connecting shaft (42), a fixed seat (43), a clamping arm (44), a first spring (45), a clamping block (46) and a supporting plate (47); the supporting frame (41) is placed on the supporting seat (13), the lower surface of the supporting frame (41) is fixedly connected with a first connecting shaft (42), the upper fixedly connected with fixing seat (43) of the supporting frame (41), the fixing seat (43) is distributed on the supporting frame (41) in an annular array mode, and the fixing seat (43) is connected with a clamping arm (44) in a rotating mode.
5. The apparatus for culturing the beneficial bacteria of the microorganisms according to claim 4, wherein: the clamping arms (44) are distributed on the fixed seat (43) in an annular array, and the upper ends of the clamping arms (44) are fixedly connected with clamping blocks (46); the inside fixedly connected with first spring (45) of fixing base (43), the upper end fixedly connected with supporting disk (47) of first spring (45), the lower surface and the centre gripping arm (44) lower extreme upper surface contact of supporting disk (47).
6. The apparatus for culturing beneficial bacteria of microorganisms according to claim 5, wherein: the adjusting structure (5) comprises a pressure lever (51), a second motor (52), a second connecting shaft (53), a second spring (54), a fixed disc (55), a bearing (56), a first fixed rod (57), a large roller (58), a second fixed rod (59) and a small roller (59 a); the second motor (52) is fixedly connected with the inner wall of the lower portion of the device shell (11), a second connecting shaft (53) is connected to an output shaft of the second motor (52) in a sliding mode, and the upper end of the second connecting shaft (53) is matched with the lower end of the first connecting shaft (42).
7. The apparatus for culturing beneficial bacteria of microorganisms according to claim 6, wherein: a second spring (54) is sleeved on an output shaft of the second motor (52), and the inner diameter of the second spring (54) is larger than the outer diameter of the second connecting shaft (53); the lower part of second connecting axle (53) is connected with fixed disk (55) through bearing (56) rotation, the symmetry is provided with first dead lever (57) on fixed disk (55), first dead lever (57) and fixed disk (55) fixed connection, and first dead lever (57) keep away from the one end of second connecting axle (53) and all rotate and be connected with big gyro wheel (58).
8. The apparatus for culturing beneficial bacteria of microorganisms according to claim 7, wherein: the pressing rod (51) is fixedly connected to the second connecting shaft (53), a second fixing rod (59) is fixedly connected to the middle of the pressing rod (51), the second fixing rod (59) is symmetrically arranged relative to the central axis of the pressing rod (51), and one end, far away from the pressing rod (51), of the second fixing rod (59) is rotatably connected with a small roller (59 a).
9. The apparatus for culturing beneficial microbes according to claim 8, wherein: the device body (1) comprises a device shell (11), a limiting groove (11a), a protective door (12), glass (12a), a handle (12b), a supporting seat (13), a baffle (14), a rolling shaft (15) and a rolling ball (16); supporting seat (13) are located the middle part of device shell (11), and rotate on supporting seat (13) and be connected with roller bearing (15), roller bearing (15) equidistant setting is on supporting seat (13), and roller bearing (15) all with the marginal position contact of support frame (41), the position department that lies in support frame (41) below on supporting seat (13) is provided with ball (16), ball (16) equidistant distribution is being annular array distribution on supporting seat (13), just ball (16) and supporting seat (13) roll connection.
10. The apparatus for culturing beneficial bacteria of microorganisms according to claim 9, wherein: the device is characterized in that a protective door (12) is rotatably connected to the device shell (11), the protective door (12) is symmetrically arranged on a vertical plane where a symmetry axis of the device shell (11) is located, glass (12a) and handles (12b) are arranged on the protective door (12), and the handles (12b) are fixedly connected with the corresponding protective door (12); the inner wall of the device shell (11) is provided with baffles (14) at positions on two sides of the large idler wheel (58), and the inner wall of the device shell (11) is provided with limit grooves (11a) at positions corresponding to the small idler wheels (59 a).
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