CN114624355B

CN114624355B - Medical science detects sampling device

Info

Publication number: CN114624355B
Application number: CN202210217150.2A
Authority: CN
Inventors: 张梦云; 郝家明; 黄山; 陈兰; 杨璐; 杨红; 周杰; 张飚; 吴佳连
Original assignee: Guizhou Ankang Clinical Laboratories Inc
Current assignee: Guizhou Ankang Clinical Laboratories Inc
Priority date: 2022-03-07
Filing date: 2022-03-07
Publication date: 2023-10-13
Anticipated expiration: 2042-03-07
Also published as: CN114624355A

Abstract

The application relates to the field of dual substance sampling, and particularly discloses a medical detection sampling device which comprises a pretreatment column, an equivalent flow dividing device and a chromatographic column; the outer wall of the pretreatment column is provided with an integrally formed annular sleeve for pretreatment separation, and two chromatographic columns, namely an A chromatographic column and a B chromatographic column, are arranged; an equivalent flow dividing device is arranged at the bottom end of the pretreatment column, and the pretreated sample is divided into two parts uniformly; the two chromatographic columns are connected with an equivalent flow dividing device, and the pretreated sample flows into the two chromatographic columns in an equivalent way; the solution of the product to be detected flowing through the chromatographic columns is a mobile phase, and the stationary phases in the two chromatographic columns are different. The beneficial effects are that: is beneficial to not losing products and saving time.

Description

Medical science detects sampling device

Technical Field

The application relates to the field of dual substance sampling, in particular to a medical detection sampling device.

Background

In the process of sampling and analyzing a detection sample, especially trace analysis, various samples are usually analyzed, and High Performance Liquid Chromatography (HPLC) is usually directly adopted in the prior art, so that the speed is high, but the method can only perform data analysis, cannot collect trace substances and has high instrument cost. In the case of the culture, two or more products are contained, and some substances can be separated by conventional separation methods such as extraction, distillation, etc., it is difficult to separate two substances (A and B) having similar chemical and physical properties, the yield is extremely small, and strict control of the loss during the separation is required. In the prior art, in order to reduce loss, a chromatographic column is used for separation, if A is obtained through separation and enrichment, B can remain in a stationary phase of the chromatographic column, and if B is obtained, a large amount of time is needed for eluting for a plurality of times; there is a great inconvenience in the case of a need for immediate inspection or other cases where both A, B products are to be used simultaneously. The utility model provides a medical science detects sampling device, can obtain A, B two kinds of material half amount earlier, save time, ensured A, B two kinds of material all not lost simultaneously.

Disclosure of Invention

The application aims to provide a medical detection sampling device, which is beneficial to not wasting products and saving detection time.

The basic scheme is as follows: the medical detection sampling device comprises a pretreatment column, an equivalent flow dividing device and a chromatographic column; the outer wall of the pretreatment column is provided with an integrally formed annular sleeve for pretreatment separation, and two chromatographic columns, namely an A chromatographic column and a B chromatographic column, are arranged; an equivalent flow dividing device is arranged at the bottom end of the pretreatment column, and the pretreated sample is divided into two parts uniformly; the two chromatographic columns are connected with an equivalent flow dividing device, and the pretreated sample flows into the two chromatographic columns in an equivalent way; the solution of the product to be detected flowing through the chromatographic columns is a mobile phase, and the stationary phases in the two chromatographic columns are different.

The beneficial effects are that: the mixed solution containing A, B product is equally divided into two parts to be injected into two chromatographic columns, A, B two substances are obtained from the two chromatographic columns at the same time, so that the time is saved, and under the condition of sufficient time, the chromatographic columns are eluted to collect the residual products in the fixed phase, so that the two products are not lost, and the method is beneficial to not losing the products and saving the time.

The first preferred scheme is as follows: as a further optimization of the basic scheme, an A heat-insulating sleeve is sleeved on the A chromatographic column, a B heat-insulating sleeve is sleeved on the B chromatographic column, and the temperatures in the two heat-insulating sleeves are different. Taking the A chromatographic column as an example, the heat-insulating sleeve increases the solubility or polarity difference of the A, B products, increases the flow rate of the A product, and is beneficial to collection.

And a second preferred scheme is as follows: as a further optimization of the first preferred embodiment, the equal-amount flow dividing device is provided as an upper chamber and a lower chamber. The lower chamber is used for accommodating the double-sided tapered column, and the upper chamber is used for accommodating the collected liquid.

And a preferred scheme III: as a further optimization of the second preferred scheme, a double-sided tapered column is arranged in the lower cavity, and the double-sided tapered column can be lifted to the upper cavity. The double-sided conical column is lifted to the upper cavity to divide the liquid into two parts in equal quantity so as to enter the corresponding chromatographic column.

The preferable scheme is as follows: as a further optimization of the third preferred aspect, the double-sided tapered pillar is provided in a side wall streamline peak shape. The mountain shape facilitates equal amount of "cutting" of the liquid.

The preferable scheme is as follows: as a further optimization of the fourth preferred embodiment, the B product in the a chromatographic column is eluted with an eluent as mobile phase. And the eluent is used for eluting the B product remained in the stationary phase in the A chromatographic column, so that the waste of the B product is avoided, and the A chromatographic column is regenerated (impurities are removed).

Drawings

FIG. 1 is a schematic diagram of an embodiment of the present application;

FIG. 2 is an enlarged view of an equal split device of the present application;

FIG. 3 is an enlarged view of the pretreatment column.

Detailed Description

The following is a further detailed description of the embodiments:

reference numerals in the drawings of the specification include: the pretreatment column 10, the annular sleeve 101, the mixed liquor collecting cavity 1011, the annular piston 102, the elastic membrane 104, the sealing cavity 1041, the second one-way air inlet valve 1402, the pressure storage cavity 105, the first one-way air inlet valve 1051, the pressure guide pipe 106, the pressure guide valve 1061, the one-way liquid inlet valve 11, the mixed liquor collecting pipe 12, the A chromatographic column 21, the A thermal insulation sleeve 210, the B chromatographic column 22, the B thermal insulation sleeve 220, the piston 23, the connecting pipe 40, the equal-amount splitting device 50, the double-sided conical column 51, the partition plate 52, the lower cavity 520, the upper cavity 521, the air leakage hole 522 and the spray pipe 53.

The embodiment is basically as shown in fig. 1 and 3: comprises a pretreatment column 10, an equivalent split device 50 and a chromatographic column; the pretreatment column 10 is used for pretreatment, and two chromatographic columns are arranged, namely an A chromatographic column 21 and a B chromatographic column 22; an equivalent flow dividing device 50 is arranged at the bottom end of the pretreatment column 10, and the pretreated sample is divided into two parts; the two chromatographic columns are connected with an equivalent flow dividing device 50, and the pretreated sample flows into the two chromatographic columns in equivalent; the mixed solution containing the product A, B to be detected flowing through the chromatographic columns is a mobile phase, and the stationary phases in the two chromatographic columns are different, so that the product A or B can flow out rapidly.

As shown in fig. 2, the equal-amount flow dividing device 50 is provided with: the equal split device 50 includes an upper chamber 521, a lower chamber 520, and a double-sided tapered post 51, the upper chamber 521 and the lower chamber 520 being separated by a baffle 52. In the process of collecting the mixed liquid, the lower cavity 520 is closed by the partition plate 52, the double-sided tapered column 51 is accommodated in the lower cavity 520, and the double-sided tapered column 51 is in sliding connection with the partition plate and can only slide up and down; the double-sided tapered post 51 is arranged at 1/2 of the position in the device, is similar to the shape of a mountain peak, has a wider lower end, gradually gathers at an upper end to form a frontal surface, and has a streamline side wall; when the mixed liquid in the upper cavity 521 collects part, the double-sided tapered column 51 slowly lifts up from the lower end of the lower cavity 520 to open the partition plate 52, cuts and gradually separates the liquid, and finally equally divides the liquid into two parts; (the partition plates 52 on the two sides are magnetic partition plates, the two partition plates are attracted to be in close contact, and the partition plate side walls are closely attached to the double-sided tapered column side walls in the process of lifting the double-sided tapered column 51, so that the mixed liquid does not flow into the lower cavity 520, the front and rear side walls of the lower cavity 520 are provided with the air leakage holes 522, and the double-sided tapered column seals the air leakage holes 520 only when the double-sided tapered column 51 is positioned at a lower position). The double-sided cone column 51 is lifted to divide the upper chamber 521 into two parts, wherein the left end is a chamber A, the right end is a chamber B, the baffle plate 52 at the lower end of the chamber A is connected with the chromatography column A21 through the connecting pipe 40, and the baffle plate 52 at the lower end of the chamber B is connected with the chromatography column B22. The double-sided tapered pole 51 lifts and drops the stored liquid in the divided upper chamber 521 a plurality of times.

The pretreatment column 10 is used for removing flocculent impurities and other interfering substances, solid substances such as carbon powder are filled in the pretreatment column 10 to serve as a permeation layer, the permeation layer is arranged on the side wall of the pretreatment column 10, water or a proper buffer solution is adopted to moisten a carrier, and the sample solution is convenient to flow down. The periphery of the pretreatment column 10 is fixed with an annular sleeve 101, and an annular piston 102 is arranged in the annular sleeve 101.

The periphery of the pretreatment column 10 is provided with an annular sleeve 101, the pretreatment column 10 is rotationally connected with the annular sleeve 101, the annular sleeve 101 is fixed, the lower end of the annular sleeve 101 is provided with a mixed liquid collecting cavity 1011, the outer wall of the pretreatment column 10 is provided with a plurality of holes, the lower part of the inner wall is provided with a filter screen, liquid in the pretreatment column 10 is centrifuged, and mixed solution containing A, B products is thrown into the mixed liquid collecting cavity 1011 through the filter screen. The top of mixing liquid collection chamber 1011 sets up annular piston 102, and the outer wall of pretreatment post 10 sets up the screw structure of similar reciprocating screw, and annular piston 102 constitutes reciprocating screw nut structure with pretreatment post 10, and annular sleeve 101 inner wall is equipped with along the guide way of vertical direction, is equipped with the direction arris of embedding guide way on the outer peripheral face of annular piston for when the pretreatment post rotates, annular piston 102 reciprocates from top to bottom. The inner wall of the annular sleeve 101 is provided with a limiting rib (not shown in the figure) for limiting the upward movement of the piston 23, and when the piston 23 moves downwards, the liquid in the mixed liquid collection cavity 1011 is pressed into the equal-quantity flow dividing device 50.

A sealing cavity 1041 and a pressure storage cavity 105 are arranged above the piston 23 on the right part, a first one-way air inlet valve 1051 is arranged on a connecting plate of the sealing cavity 1041 and the pressure storage cavity 105, an elastic membrane 104 is arranged at the lower end of the connecting plate, and a second one-way air inlet valve 1402 is arranged at the inner side wall of the sealing cavity 1041 positioned in the elastic membrane to perform one-way air inlet to the elastic membrane 104; the annular piston 102 moves upwards to compress the air in the sealing cavity 1041, so that the elastic membrane 104 is subjected to unidirectional air inlet into the pressure storage cavity 105, and the pressure in the pressure storage cavity 105 is increased; the pressure storage cavity 105 is connected with the lower cavity 520 of the equal-quantity flow dividing device 50 through the pressure guide pipe 106, the pressure guide pipe 106 is provided with a pressure guide valve 1061, the pressure guide valve 1061 is electrically connected with the time relay, the pressure in the pressure guide pipe 106 is set to jack up the double-sided tapered column 51 to cut the liquid in the equal-quantity flow dividing device 50 once every 10s, the pressure guide valve 1061 is opened and closed each time to convey the pressure to the lower cavity 520 so that the double-sided tapered column 51 reciprocates to cut the mixed liquid in the upper cavity 521, and the liquid can flow into the two chromatographic columns A, B respectively after being divided into the left cavity and the right cavity, so that the upper cavity 521 is in a cavity state and the mixed liquid flowing out of the pretreatment column 10 can be collected again.

The lower end of the annular sleeve is provided with a mixed liquor collecting pipe 12, the mixed liquor collecting pipe 12 is connected with an equivalent flow dividing device 50, and the equivalent flow dividing device 50 collects mixed liquor (comprising an A product and a B product, and A, B, which have similar properties, such as a melting point and the like, and are not easy to separate) which is pretreated by the pretreatment column 10 and contains two products. The equal split device 50 equally splits the mixed liquor into a column 21 and a column 22. The packing in the A chromatographic column is set as a stationary phase which is convenient for the A to flow out, and the packing in the B chromatographic column is set as a stationary phase which is convenient for the B substance to flow out; a column 21 is used to collect the A product and B column 22 is used to collect the B product.

The lower end of the liquid mixing collecting pipe 12 is provided with a one-way liquid inlet valve 11, and the one-way liquid inlet valve 11 can only allow the liquid above to flow down. And spray pipes 53 are arranged above the cavity A and the cavity B and are used for spraying water or solvent to wash the residual sample in the cavity into the chromatographic column.

A heat-insulating sleeve 210 is sleeved on the A chromatographic column 21, B heat-insulating sleeve 220 is sleeved on the B chromatographic column 22, the temperatures in the two heat-insulating sleeves are different, and the heat-insulating sleeves are similar to heat insulation on the outer wall of the chromatographic column by using a water bath device. A column 21 is used to separate the A product and B column 22 is used to separate the B product, taking A column 21 as an example: A. the two mixed products simultaneously enter the A chromatographic column 21, the solubility of the A product is larger, the diffusion is faster, the separation and enrichment can be rapidly carried out, the diffusion of the B product is very slow, and the B product still remains in the stationary phase in the A chromatographic column 21. The heat preservation sleeve has the function of heating the chromatographic column, so that the solubility (including polarity) difference of the A, B products is increased when the A, B products flow through, the diffusion speed difference is increased, and the A products are collected faster. The same applies to column B22.

The piston 23 below the a chromatographic column 21 is opened to collect the a product, the a product at this time is half of the added amount, the other half of the a product remains in the stationary phase of the B chromatographic column 22, under the condition that the time is sufficient, the B chromatographic column 22 needs to be eluted to obtain the other half of the a product, the eluent is added from the spray pipe 53, the a product remains in the stationary phase and is taken as the stationary phase together with the stationary phase of the B chromatographic column 22, the eluent is taken as the mobile phase, the eluent is injected into the eluent (injected into the B chromatographic column 22) for many times, the a product is finally enriched, and the a product can be obtained through reduced pressure evaporation of the eluent (the eluent is used for eluting the a product by utilizing a similar compatibility principle, and the process of eluting the a product by the eluent simultaneously partially plays a role of regenerating the chromatographic column). The same method is adopted for eluting the residual B product, and different types of eluents are adopted.

The specific implementation process is as follows:

1. complex samples were injected from the pretreatment column 10 to remove interfering substances, and a mixed solution of A, B products was collected.

2. In the pretreatment process, the double-sided tapered column 51 is lifted for multiple times to cut the mixed liquid in the upper cavity 521, the space of the upper cavity is extruded by the double-sided tapered column 51, so that the pressure is increased, the mixed liquid in the cavity A and the mixed liquid in the cavity B are pressed into the chromatographic column, and the injection quantity is the same.

3. The A product is collected below A column 21 and the B product is collected below B column 22.

4. After sufficient time, the two columns were eluted, the B product was collected below column a 21, the a product was collected below column B22, and the eluent was removed by evaporation under reduced pressure.

In this way, under the condition of limited time, A, B products can be obtained at the same time, and under the condition of sufficient time, the residual products in the chromatographic column can be collected.

The foregoing is merely exemplary embodiments of the present application, and specific structures and features that are well known in the art are not described in detail herein. It should be noted that modifications and improvements can be made by those skilled in the art without departing from the structure of the present application, and these should also be considered as the scope of the present application, which does not affect the effect of the implementation of the present application and the utility of the patent. The protection scope of the present application is subject to the content of the claims, and the description of the specific embodiments and the like in the specification can be used for explaining the content of the claims.

Claims

1. Medical science detects sampling device, its characterized in that: comprises a pretreatment column, an equivalent flow dividing device and a chromatographic column; the outer wall of the pretreatment column is provided with an integrally formed annular sleeve for pretreatment separation, and two chromatographic columns, namely an A chromatographic column and a B chromatographic column, are arranged; the bottom end of the pretreatment column is provided with an equivalent flow dividing device, the equivalent flow dividing device comprises an upper cavity, a lower cavity and a double-sided tapered column, the upper cavity and the lower cavity are separated by a baffle, the lower cavity is closed by the baffle in the process of collecting mixed liquid, the double-sided tapered column is accommodated in the lower cavity, when part of the mixed liquid in the upper cavity is collected, the double-sided tapered column slowly lifts up from the lower end in the lower cavity to jack up the baffle, the liquid is cut and separated gradually, and finally the liquid is divided into two parts in equivalent; the two chromatographic columns are connected with an equivalent flow dividing device, and the pretreated sample flows into the two chromatographic columns in an equivalent way; the solution of the product to be detected flowing through the chromatographic columns is a mobile phase, and the stationary phases in the two chromatographic columns are different.

2. The medical testing sampling device of claim 1, wherein: a heat-insulating sleeve is sleeved on the A chromatographic column, B heat-insulating sleeve is sleeved on the B chromatographic column, and the temperatures in the two heat-insulating sleeves are different.

3. The medical testing sampling device of claim 2, wherein: the double-sided tapered posts are arranged in a side wall streamline peak shape.

4. A medical testing sampling device according to claim 3, wherein: the product B in the A chromatographic column is eluted by using eluent as a mobile phase.